GCA_030464565.1 - 6 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaCHDDACFP_2 (original name was: gnl|Prokka|CHDDACFP_2)

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	terpene	1	15,660

gnlProkkaCHDDACFP_8 (original name was: gnl|Prokka|CHDDACFP_8)

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	cyclic-lactone-autoinducer	1	18,150

gnlProkkaCHDDACFP_12 (original name was: gnl|Prokka|CHDDACFP_12)

Region	Type	From	To	Most similar known cluster		Similarity
Region 12.1	T3PKS	166,426	207,595

gnlProkkaCHDDACFP_31 (original name was: gnl|Prokka|CHDDACFP_31)

Region	Type	From	To	Most similar known cluster		Similarity
Region 31.1	NRPS-like,NRPS	176,932	236,615

gnlProkkaCHDDACFP_32 (original name was: gnl|Prokka|CHDDACFP_32)

Region	Type	From	To	Most similar known cluster		Similarity
Region 32.1	RiPP-like	83,161	95,311	salivaricin CRL1328 α peptide/salivaricin CRL1328 β peptide	RiPP	16%

gnlProkkaCHDDACFP_34 (original name was: gnl|Prokka|CHDDACFP_34)

Region	Type	From	To	Most similar known cluster		Similarity
Region 34.1	cyclic-lactone-autoinducer	9,782	30,487

Region	Type	From	To	Most similar known cluster	Similarity
Region 2.1	terpene	1	15,660
Region 8.1	cyclic-lactone-autoinducer	1	18,150
Region 12.1	T3PKS	166,426	207,595
Region 31.1	NRPS-like,NRPS	176,932	236,615
Region 32.1	RiPP-like	83,161	95,311	salivaricin CRL1328 α peptide/salivaricin CRL1328 β peptide	RiPP	16%
Region 34.1	cyclic-lactone-autoinducer	9,782	30,487

Compact view

gnlProkkaCHDDACFP_2 - Region 1 - terpene

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Location: 1 - 15,660 nt. (total: 15,660 nt) Show pHMM detection rules used

Region on contig edge.

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaCHDDACFP_8 - Region 1 - cyclic-lactone-autoinducer

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Location: 1 - 18,150 nt. (total: 18,150 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

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gnlProkkaCHDDACFP_12 - Region 1 - T3PKS

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Location: 166,426 - 207,595 nt. (total: 41,170 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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gnlProkkaCHDDACFP_31 - Region 1 - NRPS,NRPS-like

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Location: 176,932 - 236,615 nt. (total: 59,684 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

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Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 176931 - 236615:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - Ala - D-Ser - Gly) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 176931 - 219349:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - Ala - D-Ser - Gly) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 180745 - 236615:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - Ala - D-Ser - Gly) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg31_195

AMP-binding (11..374): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VVYNTAVCAK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

ctg31_198

Search NORINE for peptide: strict or relaxed

AMP-binding (8..381): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SVTQAAVAGK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (1000..1372): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSHGYGCVLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (1967..2333): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLIVAAVIK
Nearest Stachelhaus code(s):: Ala DVWVVAAVIK (68% 8Å match); S-bohPhe or Phe DALIVAAVCK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (2921..3307): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4327..4705): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DITQMGIVWK
Nearest Stachelhaus code(s):: Gly DILQMGMVWK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

gnlProkkaCHDDACFP_32 - Region 1 - RiPP-like

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Location: 83,161 - 95,311 nt. (total: 12,151 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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Similar known gene clusters from MIBiG 3.1

gnlProkkaCHDDACFP_34 - Region 1 - cyclic-lactone-autoinducer

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Location: 9,782 - 30,487 nt. (total: 20,706 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.