LOCUS       c00037_gnlPro..         4175 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ALEFOAFG_37.
ACCESSION   c00037_gnlPro..
VERSION     c00037_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 19:36:31
            Original ID  :: gnl|Prokka|ALEFOAFG_37
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4175
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4175
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[322:3298]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      323..3298
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4175
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4175
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(2..124)
                     /locus_tag="ctg37_1"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPNADLDSLASIIESSKAAKGELL"
     CDS             complement(323..1057)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg37_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.1e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKS"
     CDS             complement(1058..3298)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg37_3"
                     /sec_met_domain="Gly_radical (E-value: 1.6e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.2e-282, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENEYWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             3516..4175
                     /locus_tag="ctg37_4"
                     /transl_table=11
                     /translation="MRNILFTVLFIGILSLIASPISLASKSLHKANMINLSNNAIICMH
                     QDPDGYLWIGTYDGLNLYNGKDTYVYRFELNNKNSLCSNIIHKISDAEPGFLWISTSLG
                     INKFSLKKRKVTESYPGYMESDLVATDSSGITLAICKENRISCYTPFSDGFRDLPREGI
                     GPQTVRTLFAGTDGYFYLLLTSGILKKLTPDFSNTTFNLLSEERQFHNKPILSAFYE"
ORIGIN
        1 acaataattc tcctttcgca gccttacttg attctatgat cgaggccaga gaatccaaat
       61 ccgcattggg taatggataa aaacgtttgg atatcatttc ggctatttct tgtggtgtct
      121 tcatactatt catcattttc tgatctatat cattttttat ctccttacaa aaatagatat
      181 ttgttgcata taacaaaaca aacacatatt aaatagtcaa ggttagtcta ggcaaaaaat
      241 tattactatc gtttaaaagc taatcggatt agattgtttt ccctgattag ctgttgcata
      301 atcgcaaggc ttgatcgaga gatcaggact tgttattttc tggtttgtag cgagagaata
      361 tcgctttcgc attatctaaa cgttcttttg ataagggttc cacccccttt aacggataat
      421 cgagacctaa ttgctcatat ttataggttc ccatcgtatg ataaggcaat aattccactt
      481 tgcgaaccac tttatatgaa ctgacatacc gagctaacgc ctccagccac tcgtcattat
      541 ccgtatatcc ggggacaata acatggcgaa tccaagtatc tatgccacgt ctctccaatt
      601 catccaagaa cagaagcgta ttatcctgct tgacacccgc cagtaacgga tgtaaatccg
      661 ggtttaaagt tttgatgtcc aataacacta agtccgcata atccaagact tcccaagctt
      721 ttgaggtaca tacgaaacca gatgtgtcca aagctgtgtg aagaccttgc tcttggcaca
      781 aacggaaaaa ctctttcaga aactcaggtt gcaataaagg ttccccaccg gtaacagtca
      841 cccccccacg ggcgataaaa ctcttatacc gtaagacttc cgtcaatagc tcgccgggag
      901 tcatctgata tttcaccttc cctttcgggt tccaagtatc cgggttatgg caatacaggc
      961 aacgcaacgg gcagccctgc ataaaaacga caaagcgaat tccgggacca tctacggtcc
     1021 cgaaactctc taatgaatgt atcttacctt ttatcactta cagtgaatgg ttaatggttc
     1081 ttgaaataac atccaactgt tgttcacgag tcagtttcac gaagtttacc gcatatccgg
     1141 atacacggat cgtaagttgc ggatatttct cgggatgttc catagcgtcg atcaacaaat
     1201 ccttatcaaa tacgttaaca ttcagatgct gacctccatc cggagtaaaa tagccatcca
     1261 ataagctaac cagattattg atctgcactt cacgctcctt tcccaacgta gccggagaaa
     1321 cagcgaaagt ataagagata ccgtcgtgcg catgctggaa tggaagcttc gctaccgaag
     1381 ctagcgcagc gatagcacct ttcgtatcac gaccgttcat agggttcgcg cccggagcga
     1441 aaggagttcc accgatacgg ccatccggtg tcgtaccagt cttcttacca tataccacat
     1501 tagaggtgat cgtaaggatg gactgcgtag gtttcgcatg gcgataggtc tcgtgctgac
     1561 gcaaatattc catgaacttc tcggtgatct ctacagcgat actatccgta cgatcatcgt
     1621 tattaccgaa aggaacatat tcgccctcac gttcgaaatc aaccgccaag ccacgctcgt
     1681 cacggatcac acgtactttc gtatcacgga tagcagccaa cgaatcggca acgatagaaa
     1741 gaccggcgat acctgtcgca cggatacgct ctacatcccc atcgtgaagc gacatctcga
     1801 atgcctcata cgcgtattta tcatgcatat aatggattat cttcaaggcg ttcacataca
     1861 ctttcgccaa ccagcgcatc atttgctcat acttagccat cacctcatcg tattccagat
     1921 actcgccgcg aaccggctcg aacatcgggg ctacctgtac gccgctgcgc tcatcccgtc
     1981 ctccgttgat cgcatataat aaacatttcg ccaaattcgc acgggctccg aagaactgca
     2041 tctgcttacc gatcttcata ggagaaacgc aacaagcgat accataatca tctccataat
     2101 ccggacgcat caaatcgtcg ttctcatatt ggatagccga cgtatcgata gagaccttgg
     2161 cacagaaacg tttccagttc tcgggagcgt tcttaaacca cagaaccgtc aaattgggtt
     2221 ctggtgccgg acctagatta tataatgtat ggagataacg ataggaagtt cttgttacca
     2281 aagaacggcc atccactcct tggccgccca aggactctgt tacccaaacc ggatcaccgg
     2341 agaacaaatc attgtattcc ggcgtacgta agaaacgaac gatacgcaac ttaatgatca
     2401 gctggtctac caattcttgc gcctcctctt cggtcaactt accctcacgg atgtctttct
     2461 cgatataaat atccaaaaac gtagaagtac gtccgataga catcgccgcg ccatcttggt
     2521 cttttaccgc cgccagataa gctaagtaca caaactgaac tgcctcacga gcgttttgtg
     2581 ccggacgagt cacatcaaag ccatagctag cgcacatttt ctcgaacgct ttcaaagcct
     2641 tgatctgttc agaaatctcc tcacggctct ggatgatctc ttccgtgatc tcttggatat
     2701 ccaaacgatt caagaaacgc tttttctcct cgatcaagat tgccgtacca tacagagcga
     2761 cacgacgata gtcaccgata atacgtcctc tgccatacgc atcgggcaga ccggtgataa
     2821 tagccgaacg acgtgcggca atcatctctt ccgtatatgc ggagaaaacg ccctcgttat
     2881 gagtcttacg atacttcgta aagatctctt tcgtcatcgg gtccaaatga taaccataag
     2941 cttccaagct attctcgacc atacggatac cgcctttcgg gaagatacca cgtttcagcg
     3001 gagcatcggt ttgtaaccca acgatcacct cattctcctt atcaatatag cccgggccat
     3061 atacatcgat cccttgaggt aattttgtct cggcgtcata aactcccttt tccctctcga
     3121 ccttaaacat ctcggtcagc ttgttccaga ccttacgggt cttctcggaa gaagcgacta
     3181 aaaaggaatc atccccttca taaggcgtat agttactctg gataaaatct cttacattga
     3241 tctctctctt ccaggtctca cccttaaagt gatcccagta ctcattctca aatttcataa
     3301 acaatcttat ctaataccac atttttaccg gcacaaaggt aaaactttta tcctcatgtt
     3361 cctgtaacat ttgcgacacc cctctaaaat ccctataaat aggtattaaa aatatacaag
     3421 aagaaacggg atagtaatgc gatgctttgc acgaagtctc cattttattc actatctttg
     3481 cctacagaac gtaaaagtta atctggatca aacacatgag aaatatcctg tttaccgttt
     3541 tatttatagg aatactatct ttaatcgcat ctcctatctc tctcgcgtct aaatctttgc
     3601 ataaggcaaa tatgattaat ttgtcgaaca atgccattat ttgcatgcat caagatccgg
     3661 atggctattt gtggatcggg acttacgacg gacttaactt atacaacgga aaagatacgt
     3721 atgtatatcg tttcgaatta aataacaaaa actctctttg tagtaatatc attcacaaaa
     3781 tatccgacgc agaacccggc tttttatgga tatcaacctc tttaggtatc aataaattct
     3841 cgctaaagaa acggaaagtc accgagtctt acccgggata catggagagt gatctggtag
     3901 cgaccgattc atccggtatc acattagcca tttgtaaaga aaaccggata tcttgttaca
     3961 ccccatttag cgatggattc cgggatctgc cgagagaagg gataggccct caaaccgtac
     4021 ggactttatt cgccggaaca gacggatatt tttatttact attgacctcg ggaatactga
     4081 aaaagttaac accggatttc tccaatacaa cttttaattt actatcggaa gaacgccaat
     4141 tccataataa accgatctta tccgcatttt acgag
//
