LOCUS       c00329_gnlPro..         6177 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KKHFPLKO_329.
ACCESSION   c00329_gnlPro..
VERSION     c00329_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 16:59:49
            Original ID  :: gnl|Prokka|KKHFPLKO_329
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6177
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6177
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2469:5425]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2470..5425
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6177
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6177
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..166
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg329_1"
                     /sec_met_domain="Fn3-like (E-value: 3.2e-15, bitscore:
                     44.3, seeds: 729, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LKKGETKRVRILLDEEAFAYYDVESHRFVVEKGTFEILAGPSSAD
                     LPLKATVVL"
     CDS             197..2401
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 84.6; E-value: 1.3e-26)"
                     /gene_kind="other"
                     /locus_tag="ctg329_2"
                     /sec_met_domain="Fn3-like (E-value: 8.2e-28, bitscore:
                     84.6, seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3 (E-value: 4.3e-74, bitscore:
                     237.7, seeds: 11, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 3e-53, bitscore:
                     168.9, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKIIILMVCLWGLGNTFTSAQTTEKEKFISSLMKQMTLDEKIGQ
                     LAQCTYRGNFTGPDGGNIPKEEYVRQGRIGSMLNVIGVKDIRRYQELALQSRLRIPLIF
                     GLDVIHGYRTGFPLPLAEAASFDLAMIEEAARYNALESAADGLNWVFAPMVDISWDARW
                     GRVMEGAGEDPYYGSRVAEARVRGLQGDDLADTTTVMACMKHFAGYGAPIAGKEYNSVD
                     MSMGHFANFYMPPYKAAIKVGAATVMSAFNDFNNIPCTANDFLLNRLLREQWGFKGFVV
                     SDYNSVEELVNHRYAVDKKDAAAKALNAGLDMEMVSTCYLTYLKELVQEGKVKESVLDD
                     AVRCILEKKYELGLFEDPFRYCNPERAARILNAPEVRNASLRMAERSVVLLENRESVLP
                     LTAQTRKIALIGGLSKSQYDMAGAWAATTDRNKVVTLYDALVRKGLEVSYAEGYDMKSN
                     QLTGLQEALQAAEASDVIVVAVGERFGQSGEKASKVNIAIPEGQQKLVSELAKTGKPVI
                     ALVMCGRPVIFNEIREHADAVLCTWWLGSEAGNAICNVLWGEYNPSAKLPMTFPAHVGQ
                     IPIYYQNKSTGRPTALKQTYKASYIDASTEPAYPFGYGLSYTDFGYSDLKLLPGKAAGV
                     HTEVSVKVTNTGKCAGEEVVQLYVQDKVASVTRPIKELKGFRKIHLNVGESKTVAFQIT
                     DDALGFYDNEMNYMVEPGDFVFMVGGSSDDVQQITYNLKE"
     CDS             complement(2470..3195)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg329_3"
                     /sec_met_domain="Radical_SAM (E-value: 6e-32, bitscore:
                     99.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDTRLLGESLGKYQQIQRVEILPYHRLGVHKYEAMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(3197..5425)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg329_4"
                     /sec_met_domain="Gly_radical (E-value: 2.2e-47, bitscore:
                     148.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1170.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKIDETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 cttgaagaag ggtgaaacca agcgcgttcg gatattgctg gatgaggaag cctttgccta
       61 ctatgatgtg gaatcacatc ggtttgtagt ggaaaagggg acttttgaga ttctggcagg
      121 tccttcttca gctgatttac cattgaaggc aactgttgtt ttatgataaa ataaatatca
      181 aaacaagaat ggcagtatga aaaagataat aatactgatg gtttgtctgt ggggacttgg
      241 taacaccttt acctcggcac aaactacgga gaaggagaaa ttcatctcaa gcttgatgaa
      301 gcagatgacg ctggatgaaa agataggcca gcttgcacaa tgcacctacc gagggaattt
      361 taccggaccc gacggtggaa atattcctaa agaagagtat gtccggcaag gcagaatagg
      421 ctctatgctg aatgtgatag gagtaaagga tatacgccgt tatcaagagc ttgctttgca
      481 atcgcgtttg cgcattcctt taattttcgg actggatgtt atccacggct atcgtaccgg
      541 atttccgctt ccgcttgccg aggctgcaag tttcgatttg gccatgattg aggaagctgc
      601 acgctacaat gctttggaaa gtgccgccga tggtttgaac tgggtttttg ccccgatggt
      661 ggatatttcg tgggacgcac gttggggacg cgtcatggaa ggggccggtg aggaccctta
      721 ttatggttct cgggtagcgg aggcgcgtgt cagaggcttg caaggagacg atttggcaga
      781 cacgacgact gtcatggcat gtatgaaaca ttttgccgga tatggtgcac cgatagcagg
      841 aaaggagtat aatagtgtgg atatgtcaat gggccacttt gccaattttt atatgcctcc
      901 ctataaggcg gccataaagg taggagcagc taccgtgatg agtgctttca atgattttaa
      961 taatattcct tgtactgcca atgatttctt gctcaaccgg ctattgagag agcagtgggg
     1021 atttaaggga tttgttgtgt ccgattataa ttcggtagag gaattggtga accaccgtta
     1081 tgctgttgac aagaaagatg ccgcagccaa agcgctgaat gctggtctgg acatggagat
     1141 ggtaagtacc tgctacttga cttatctgaa ggagcttgtt caagaaggga aagtaaagga
     1201 gagtgtgctt gatgatgcag tacggtgtat attggagaag aaatatgagt tgggattgtt
     1261 tgaggaccct tttcgctact gtaatccgga aagagcggcc agaatactca atgcgcccga
     1321 agtccgtaat gcttcgctcc gtatggccga gcgttccgtt gtattgctgg agaataggga
     1381 atctgtgctt ccgttgacgg cacagacgag gaagatagcc ttgataggag gactctcaaa
     1441 atcccagtac gatatggcag gtgcttgggc tgccactacg gaccggaaca aagtggtgac
     1501 cttatatgat gcattggtcc ggaaaggact ggaggtgagc tatgctgaag gctatgatat
     1561 gaagagcaat cagttgaccg gattgcagga agcattacag gctgctgagg cttcggatgt
     1621 cattgttgtg gcggtaggcg agagattcgg tcagagtggc gagaaggctt ccaaagtgaa
     1681 tatagccata cccgaggggc agcagaagct ggtgtcggaa ctggcaaaga ctggaaaacc
     1741 tgtcattgct cttgtcatgt gtgggcgtcc ggtaatcttc aatgagataa gggaacatgc
     1801 tgatgcggta ctgtgtactt ggtggctggg aagtgaggcc ggcaatgcca tatgcaatgt
     1861 gttgtgggga gagtataatc cttcggctaa attgcccatg acatttcctg cacacgtagg
     1921 acagattccc atttattatc agaataagag caccggccgc cctaccgcat tgaaacagac
     1981 ctataaggca tcttatatcg atgcttccac tgagcctgcc tatcctttcg gatatgggct
     2041 ttcgtacacg gattttggat attctgattt gaaactgctg ccgggcaagg ctgcaggcgt
     2101 ccatacggag gtgtcggtaa aagtgactaa taccggtaag tgtgcaggag aggaagtagt
     2161 tcaattgtat gtgcaggata aggtagcctc tgttacgcgt ccgataaagg aattgaaggg
     2221 cttcaggaaa attcatctga atgtgggtga aagcaaaact gtagcttttc agataactga
     2281 cgatgcactg ggattctatg acaatgagat gaattatatg gtagaacctg gtgatttcgt
     2341 ctttatggtg ggtggcagta gtgatgatgt acagcagatt acctataact tgaaagaata
     2401 ggattcttac cctataaatg aaaagcaagg ctgtctggaa tcatatccgg acagccttgc
     2461 ttttctgttt tagttggtta tcacattcgt gaaatactct ttgaacagtt tttctgcccg
     2521 ttccagttgt tccggagtat tttccttcac ttctttcaat ttgtaatccc atcccatggc
     2581 ttcgtatttg tgaacaccca gacggtgata aggaagtatt tccacccgtt gtatctgctg
     2641 gtatttgcca agactttcac ccagcaggcg ggtgtcttct tccgaatcgc tgtaaccggg
     2701 gaccagtaca tagcgcagcc agaaggggtg ttcatgttct tcgagccagg cagcggtgcg
     2761 gagtgtctgt tcgttgcttc gtcccgtcag tgtacggtgg cgttcgggat tgaattgctt
     2821 gatgtccagc agcacgaggt cagtcagact aaagagctct tccacctttt cattccagat
     2881 gcctccgtta ctgtccagac agatatggat gccgttcgcc ttcagctcct tgaaaagagg
     2941 aatcagcgct tctgcttgca gggtaggctc accgccggag aaagtaatac ctcctttctt
     3001 cccgaagaat gccttttggc tgatagccat ttgcagaatc tcttccggag tggtgggagt
     3061 gcctcctttt acatcaatcg tatccggatt ggcacaatac agacaacgga acggacatcc
     3121 ttgcaggaaa acgacgagcc ggagacccgg cccgtcgaat gttcccatac tttcgtagga
     3181 gtgtacattt atcatattac attctttcgt ggaatgaacg gctgatgact tccaactgat
     3241 gctcgcggct caacttcacg aagttcacgg catagccgga aacgcggatg gtgagttgcg
     3301 gatacttttc aggatgttcc atggcatctt ccagcatttc gcggttcagt acgtttacgt
     3361 tcaaatggtg ggcacctttg gtgaagtagc cgtccatcat ggttaccagg ttctctacac
     3421 gttcttccgg tgtcgggccc agtgatttgg gaacaatgga gaacgtattg ctgatgccgt
     3481 cctgcgagtc gcggtagcgc aatttggcta cggagctcaa ggaagcgatg gcaccgttct
     3541 tgtcacgtcc gtgcattggg ttggcacccg gagcaaaggc aacacctttg gcgcgtccgt
     3601 ccggagtggc accggtcttc ttgccgtaca tcacgttgga ggtgatagtc agcaaggaaa
     3661 gggtgggacg ggcattcttg tagacaggca gctttttcag ttcttcgctg aaatagtaaa
     3721 cgaggtctac tcccaggtgg tccacacggt cgtcattatt gccgaagcag gggaatgagc
     3781 cttcggtgtc gaaaccttcg gtcaggccga tttcgttgcg gcgtgcagtg actttggcat
     3841 atttgatggc ggacagtgag tctatggcga tggaaaggcc ggcaacaccg tatgccagat
     3901 tgatgcgtgg gtcggtatcg atgaatgcca tctgggcttt ctcataataa tacttatcat
     3961 gcatgtagtg gatgatattc atggcctcgt tgtagacacg ggctatttca gtcagtacct
     4021 tcttgtagtt ggccataact tcctcaaact tcagtgtctc gccggtcaat acgggaatac
     4081 ctttcaccat gacagttccg gtgttttcgc aacggccacc gttgatggca agcagcaggg
     4141 ccttggcaag gttggcacgt gcgccgaaga actggatttg cttgccgatg gcttggtagg
     4201 atacacagca ggcaatgccg taatcgtcgc agttgcgcac ttcgcgcatc aggttgtcgt
     4261 tttcgtactg gatggagctg gtatctacag aaactttggc gcagaaatcc ttgaagcctt
     4321 ccggcagttc gggactccac aatacggtca agttcggttc cggagaagga ccgaggttgt
     4381 atagcgtctg caaaaagcgg aaagaagtct tggtgacctt cacgcgtccg tcgttgaagc
     4441 gtccgccgat ggcttcggtc acccaagtcg ggtcgccggc aaagatatca ttgtacgact
     4501 gcatgcgcag atggcgtacc atacgtagtt tgatgacgaa ctggtctatc agttcctgtg
     4561 caaacgtctc gtcaatcttg ccgtgagcca aatcgtattc gatgaaaatg tcgaggaacg
     4621 aagatacgtt acccagtgac atggctgctc cgtcctgctc tttgatggca gcaagatatg
     4681 ccatgtatac ccattgcacg gcctcttgag ctgatgtagc cggatggctg aggtcgagtc
     4741 cgtaatattc gcccataacc ttgatgtcct tcaaagcttt gatttgttct gccacttctt
     4801 cgcgtagacg gatgcgtgct tcggtcatcg ggccggtgag gttgcgcaag tcctcctgct
     4861 tggcttcaat cagacggtcg atgccgtaga gagccaatcg gcggtagtcg ccgatgatac
     4921 gtccgcgggc atagttgtcg ggcaatccgg taaggaatcc cagtgagcgg aaggaacgga
     4981 tttcttcggt atatacatcg aatacgccgt cgttatgtgt tttgcggtaa tgggtgaaga
     5041 tgtcttttac tttatcgtcc acttccactc cgttttcacg gcatgctttt gctaccacgt
     5101 tgatgcctcc gaaaggtttg atggatcgtt tcaacagttc atcagtctgc aaaccgacaa
     5161 taagttcatt gtccttatca atatacccgg ctttatgaga agtgatggta gatacggtgg
     5221 tattgtccag tgaacggacg ccattgttgt tcctttcttc ttcgagagcc ttcagacaga
     5281 tgtcccagat acgtttggta cgttcggtag gtccctgcaa gaaagatgca tccccggtat
     5341 agggtgcgat gtttttactg acgaaatcac tgacattgat ttctttattc caaagcccat
     5401 tggtaaaggt tttgttcaat tccataaaat ttgtttttgt tagtaggtta gataatgtta
     5461 tcctttagag cgcacaaaaa tactgaataa attccgtaat tttataagag atacttctct
     5521 ttttatgttg aacagcataa aataactatt attaggtagt gaatgtccgg gggagtagag
     5581 atgttaatac tttctttata tgtggtttat gaattgaaac aagaaccgaa taataatctg
     5641 cattatttac aaatgatgta ggtttaagga acagtcgtat tctgaaccta attcagtcgt
     5701 atagtgaatg ccattcagtc gtatactgaa tagggttcaa tcgtatactg aatgtggctc
     5761 tttgtaaata tactgatagc cagtaagtta taaaactctg tcttgccctt gataaatttc
     5821 aacaaatagc ctaaatgccg ttatgttcgt tccgtagtgg gtataaacaa gaattttggt
     5881 ataattgctc tgtttttgtg cagaaaagtt tgtagaataa aaaatgattt gtatctttgc
     5941 accgctttaa aagaaaagta cttctgaaaa agaagttttg gagaggtggc agagtggtcg
     6001 attgcggcgg tcttgaaaac cgttgtgctg cgaggcaccc ggggttcgaa tccctgtctc
     6061 tccgcaacaa acgctgaaaa tcagcagatt gcaaaacaaa cacccagttt tacacccaag
     6121 aatgtaaagt cgggtgtttt tgttttattt aagataatac aaccactaca taataaa
//
