LOCUS       gnlProkkaAPPAEHGL_29   14209 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|APPAEHGL_29.
ACCESSION   gnlProkkaAPPAEHGL_29
VERSION     gnlProkkaAPPAEHGL_29
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 15:58:05
            Original ID  :: gnl|Prokka|APPAEHGL_29
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14209
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14209
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[1424:9209]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1425..9209
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..14209
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..14209
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             383..907
                     /locus_tag="ctg29_1"
                     /transl_table=11
                     /translation="MSEQQKYWFAARTRDKQEFAIRDSLEKLKTELDLNYYLPTQFVIR
                     QLKYRRKRVEVPVIKNLIFIQATKQDACDISNKYNIQLFYMKDLLTRAMLIVPDKQMQD
                     FIFVMDLDPNGVSFDNDHLSVGSRVQVVKGDFCGVEGELASEANKTYVVIRIAGVLSAS
                     VKVPKSYLRVI"
     CDS             911..1393
                     /locus_tag="ctg29_2"
                     /transl_table=11
                     /translation="MNTLTSQIEQLQSLAHELLYLGVDGAPIYTDHFRQLNKEVLEQSD
                     ALYPQRGATPEEEANICLALLMGYNATIYNQGDKEEKKQVVLNRCWDVLDQLPATLLKC
                     QLLTYCYGEVFEEELAKEAHTIIESWKDKELSEDKKEAINNLRNLEENPYPYSEME"
     CDS             1425..2318
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 268.8; E-value:
                     9.4e-82)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg29_3"
                     /sec_met_domain="NTP_transf_3 (E-value: 1.8e-12, bitscore:
                     41.5, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGIVLAGGSGTRLYPITKGVSKQLLPIFDKPMIYYPISVLMLAG
                     IREILIISTPYDLPGFQRLLGDGSDFGVHFEYAEQPSPDGLAQAFIIGEKFIGSDSVCL
                     VLGDNIFYGQGFTRMLREAVHSAESENKATVFGYWVSDPERYGVAEFDQEGNVLSIEEK
                     PQIPKSHYAVVGLYFYPNKVVEIAQNIKPSPRGELEITTVNQQFLLDQELKVKLLGRGF
                     AWLDTGTHDSLSEASTFIEVIEKRQGLKVACLEGIAFRQGWISSEKMKVLAQPMLKSQY
                     GQYMLKMIDITTSNEE"
     CDS             2491..3795
                     /locus_tag="ctg29_4"
                     /transl_table=11
                     /translation="MIYMFTRMIRSLLSRSPLYVLALYASVLIFLVYTCAYAYRKPFTA
                     AIYEGEILWGFDVKILYVLSEIIGYALSKFIGVRILPSMKAGHRIYYIIGLLTFSEVAL
                     LGFALLPVPLKVCSIFLSGLPLGMIWGVIFSYIEGRRISEILNVGLSVALIVSSGLVKT
                     LGQFVMDNLHVSEYWMPFTTGLLVYPVMLLCSWLLNQIPAPNEQDIIQRTKRAPMDGKD
                     RRKFLRQFFWGICMLVVFYGALTVFRELRDSFAADIWKELHIEGAMIFTQTEVPIAVFV
                     LVLMFLIVFVRNNRLALNIIYCIAVTGGSLMVFSTLLYVYGFLSPIWWMILSGLGLYMG
                     YIPFTYLIERLIASLRVVSTAVFIIYLADSFGYLGTTGVFMVKNFVSADISWTVMLMRT
                     ALFSGFVSVLSIFAIYCYFKQQLNSLILIPSEIHD"
     CDS             3809..4507
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg29_5"
                     /sec_met_domain="NTP_transf_3 (E-value: 2.2e-13, bitscore:
                     44.5, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAGLGTRFGHYTELVPKGFVEVGGKPMIIRSIETLLSCGIERII
                     LGTGYKKEAYEALQADFPQIETCFSPRYADTNSMYTLYNTRDVIGDDSFLLLESDLVFE
                     RKALLSLLDDEFPDVMLVSSLTKFQDQYYVEYDRNHILTSCSVDKDALEAKGELVGIHK
                     LSNTFYRRMCADYATILESQPKLGYEYELLRMSRSVSPVRVLRVEGLKWYEIDDEADLS
                     YAEEHIIRYC"
     CDS             4527..5828
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 214.1;
                     E-value: 3.9e-65)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg29_6"
                     /sec_met_domain="CTP_transf_like (E-value: 5.1e-12,
                     bitscore: 39.9, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 1.9e-121, bitscore:
                     398.2, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 9.4e-64, bitscore:
                     208.8, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKKIYVGMTADIMHPGLIHIIHEATKYGDVIVGLLTDKAIAEHK
                     RLPYLTYDQRKEVVENIKGVCEVVPQEDWSYVPNLEKLKPDYIIHGDDWKTGPLREVRE
                     QVFEVMNAQGGKVIEIPYTRGINSSSLDKDIKAIGTTPDVRLKSLRRLIQAKPVVRILE
                     AHDGLCGLIIENLEVQKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAVIIEDKVGLKKNSLFGTDAIQTQDS
                     IEGFCDKIRAGKNAQVTGDFMIIARIESLIAGKPMSDALERAYAYVQAGADGIMIHSKD
                     KSGEDIREFCRTFRKEYAHVPIVVVPTTYDHVHESELHEWGANVVIYANHLLRAAYPAM
                     MNVARTILENERAEEVRPLCMPIKEILELIPGTK"
     CDS             5840..6976
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 71.8;
                     E-value: 7.9e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg29_7"
                     /sec_met_domain="TPP_enzyme_N (E-value: 3.1e-21, bitscore:
                     69.4, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 8.1e-20, bitscore:
                     64.8, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVSVEKIYDTFLHHGVDFFTGVPDSLLKNICAYITDHAPRGKHII
                     AANEGSAVGIACGYYMASGNVPLVYMQNSGLGNTVNPLLSLADEKVYSLPLLLLVGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMKIPFIILDTDEDEALAQIHDAVVSAKSRSIPHAIV
                     IRKDTFGKYKLRQEALNDYPLSREEALMQVVDHLGDNDIVVSTTGKLSRELFEYRESKG
                     QGHARDFLTVGSMGHSSSIALGVALEKPDRRVYCLDGDGAFIMHMGAVSNIGDLSPKNY
                     RHVLFNNGAHESVGGQPTLGFRLDIASIAKGCGYAHVLTASDKEGLSCALEKLSGLSGP
                     VLLEIKVRIDSRDDLGRPTTTPVENKEHFMAFLKDTHS"
     CDS             6973..8097
                     /NRPS_PKS="Domain: Aminotran_5 (30-301). E-value: 6.1e-34.
                     Score: 109.3. Matches aSDomain:
                     nrpspksdomains_ctg29_8_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 80.4; E-value:
                     2e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg29_8"
                     /sec_met_domain="Aminotran_5 (E-value: 1.5e-30, bitscore:
                     100.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIERKILLNPGPATTTDTVKLAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGDLSKHTAVLFCGSGTINIDICLNSLLPADKKVLVVNNGAYSTRAVEVCEYY
                     GLPHIDLKFSVYELPDLSVIEKTLSDNPDIALVYTTHHETGTGILNPIREIGKIAHSHG
                     AVFVVDTTSSLGMIPFDIGKDNVDFCMASAQKGLQAMTGLSFIIGDESLIRASRDYPKR
                     SYYCNLYLQYDYFEKTGEMHFTPPVQTVYAARQALDEYFAVGEEAKFARHKRVFEAIHK
                     GLQELGLQYAIKREWQSGLVVSVLYPDDPLWDFDKVHDYCYERGFTIYPGKISTTNTFR
                     LCALGEIDVEDIERFFEVMREAISFLKISFKNIV"
     aSDomain        7063..7875
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg29_8_Aminotran_5.1"
                     /evalue="6.10E-34"
                     /label="ctg29_8_Aminotran_5.1"
                     /locus_tag="ctg29_8"
                     /protein_end="301"
                     /protein_start="30"
                     /score="109.3"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGDLSKHTAVLFCGSGTINIDICL
                     NSLLPADKKVLVVNNGAYSTRAVEVCEYYGLPHIDLKFSVYELPDLSVIEKTLSDNPDI
                     ALVYTTHHETGTGILNPIREIGKIAHSHGAVFVVDTTSSLGMIPFDIGKDNVDFCMASA
                     QKGLQAMTGLSFIIGDESLIRASRDYPKRSYYCNLYLQYDYFEKTGEMHFTPPVQTVYA
                     ARQALDEYFAVGEEAKFARHKRVFEAIHKGLQELGLQYAIKREWQSGLV"
     CDS             8100..9209
                     /NRPS_PKS="Domain: Aminotran_1_2 (37-163). E-value:
                     1.2e-06. Score: 19.8. Matches aSDomain:
                     nrpspksdomains_ctg29_9_Aminotran_1_2.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056:DegT/DnrJ/EryC1/StrS aminotransferase (Score:
                     280.3; E-value: 4.2e-85)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg29_9"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 2.5e-94,
                     bitscore: 310.1, seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 4.2e-06, bitscore:
                     19.8, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEENQITVTSPLLPSLDDFLPYLEDIWKRKWITNNGYYHQELEKA
                     LCQYLKVPYISLFTNGTLPLICSLQALRITGEVITTPYSFVATTHALWWNGIKPVFVDI
                     DPKTCNIDPNKIEAAITPKTTAIMPVHVYGNPCDFIAIQDIADKYGLKVIYDAAHAFGV
                     EVDGKSLLEFGDMSTLSFHATKVYNTIEGGALVCHDRQIKERVDFLKNFGFAGETQIVA
                     PGINGKIDEIRSAYGLLNLKAVDSAIEYRHQISKRYREGLGGIDGISYMPEMHNIRHNY
                     SYFPIFIDSVKYGITRDELYEKMKRNGVCGRRYFYPLITNFSTYCGLDSAQISNLPVAN
                     RIANSVICLPLHTGLTSIDVERIIELLKK"
     aSDomain        8211..8588
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg29_9_Aminotran_1_2.1"
                     /evalue="1.20E-06"
                     /label="ctg29_9_Aminotran_1_2.1"
                     /locus_tag="ctg29_9"
                     /protein_end="163"
                     /protein_start="37"
                     /score="19.8"
                     /tool="antismash"
                     /translation="YHQELEKALCQYLKVPYISLFTNGTLPLICSLQALRITGEVITTP
                     YSFVATTHALWWNGIKPVFVDIDPKTCNIDPNKIEAAITPKTTAIMPVHVYGNPCDFIA
                     IQDIADKYGLKVIYDAAHAFGV"
     CDS             9206..9754
                     /locus_tag="ctg29_10"
                     /transl_table=11
                     /translation="MTSFYSEKELANIGLAVYGTNVLISRKVSFYSPKNIYIGNNVRID
                     DFCILSGKILIGSNIHISAYVAIYGGYGVVLEDYVGISPRTTIYSAMDDFSGDYLVGPI
                     HDKRLTHVIGGEVRICKFAHIGANTIVYPGCSIGEGTVVGAMSMVRKSLDAWGVYIGIP
                     AKFLKNRNKNMQSLIYGKE"
     CDS             9741..10685
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg29_11"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.1e-20,
                     bitscore: 67.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGKNNRIPLVSVCCMTYNHDKFISQCLDGIIQQKGNFSIEILIHD
                     DASSDHTRQIIEFYRDKYPDIIKPLYQEINQYSRGVNIWNTYMFPLVKGEYIAICDGDD
                     YWIDQYKLEKQITFLQKYADYNLVYTNKIVINELGTRVIRNHPKYLSGNLFVNLLSGGN
                     PITTSTVCIRTHDAFEIMDIVNSIPFRTVMGDLQLWLALAIKGKFKYLAFKSTVYRLSS
                     ISLSHSLSVNREIRFSKHEYFIKKYFADIFQVNIDKKILERTYYKNTIRRLFSFRKEIF
                     LSYVFKSVKKDPFLIFDIKIITLVGVKLLKSIL"
     CDS             10682..11848
                     /locus_tag="ctg29_12"
                     /transl_table=11
                     /translation="MKVLLVFIDMMRTDLLSVYNQNVKDKTSFDLLLEKIGGCIYTNCY
                     TPAPDTPRSLACLQTGLHPYFNGCNTRIKWPKYYVKEEIDTIFDILSNNKILINYYAPK
                     VDLDTGALSIKEGSVVNLFTNIEKFTSNILQSTCDEMDFLTLDDYHWAIDDYGATHKAV
                     KIGHNIISNVLNTFFRTHSYDEFDHIFFFSDHGHKLENEIALMESALELLRDDRAKILM
                     VHRSKRNSGLSIDNRLCSIQDLYATILSIFHLNYDHRDSLSLLSTSKSDVHDHLIIEDH
                     ADFTVSLNQVISVWRYISDAGSFYTNVCKYSKEGYCELSNSTIISLIQQKSPTYLDYER
                     QMLILKEYSKMSEKYCTYSNGGKRLGRKKQVLLKVKNKCYRFLIKCFR"
     CDS             11845..13101
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg29_13"
                     /sec_met_domain="Polysacc_synt (E-value: 4.5e-12, bitscore:
                     39.6, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRSRFIELITVTASSIIELSTTLLITKIVAVQLSKDGYGFYSLIL
                     SVVALFSLLPFSSLHVGIERYIIEYKNRNKFARFYASIYLIHIVFFVLYLLLLLPLSPF
                     LTGEWNEIWEIVYLFFITKVYKQFVFCILNINRKRISILYARIIDLSIQLILLYFFLIK
                     GLSIQEILYASIVSSLLTIGYLVYIDSHNIYLKKVRVSDFLLVLKDVFSYSYPLIIWGI
                     FAWIQSMIGRWFVDFYLTKSDVANFSMIFSLSLLPSTAISAIIGSYFVPIAYSNEAKST
                     GFIGRYINKILFAVSLVWLVIILTFLFLGEYIIVLFLDVKYLNIYWYLFWGLLGTGVYT
                     LGQLSVYELYYYKKVKYLLLPNLLPGLLTIVGCMIIVPFWGFDGAVYVNVFTYVISGIL
                     TLYCTLYYGRKIKIIYGNC"
     CDS             13088..13960
                     /locus_tag="ctg29_14"
                     /transl_table=11
                     /translation="MVIAKLFGGLGNQMFIYAAAKGIAQISNQKLTFDIYTGFEDDSRF
                     RRVYELKQFNLSVQESRRWMSFRYPLGRILRKISRKIGFCIPLVNFKFIVEKKPYHFQN
                     EIMRIASFSSIYLEGYFQSYKYFSKIEAQIREDFKFTKEVIGSVEKEASFITNSRYTPV
                     AIGVRRYSEMKGEFGELAVVEHDYYDAAIKYIANKVPNLIFIVFSEDIDWVKKNLKLDY
                     PVYFVTSKKGELAAIQDMYLMSLCNHHIISNSSFYWWGAYLASTNNHIVIAPSVFLNKD
                     CTPIDWVII"
ORIGIN
        1 tcttgttatt agttatttat cttaagatat aggtgttgtt tcaaaaaacg ttcgtttgat
       61 tgaacaaagg taaggagtct gttttaatgt acaaaatatt tagtggataa aattttatca
      121 aagattttat attgttgatt ttcaaacaca tatagagcaa aaaaagttct ctgctcagca
      181 actttacaat attcattttt tcatcgaaaa aaactcaaaa aatgctgttt caaaagacga
      241 acgttttttg aaacacattc gttcgttctc aacttctaaa aatgtttcgt aaaatttgac
      301 ggtttgaaag agaaaagatg tctcgttcaa accgcgaagc gcacccgaaa caaaggaaaa
      361 tttccccaag tataaatcgt aaatgtctga acaacagaag tattggtttg ccgcccgtac
      421 ccgagataaa caagagtttg ctattcgtga ctctcttgaa aaattgaaga ctgaacttga
      481 tctcaattac tatcttccca ctcagtttgt catccggcag ttgaaatatc gtcgaaaacg
      541 ggtggaagtc cctgttatta agaatcttat cttcatccaa gctaccaagc aagatgcttg
      601 tgatatttct aataaataca atatccagct tttttatatg aaagacctgc ttaccagggc
      661 catgctgata gttcctgata aacagatgca ggacttcata tttgtcatgg atttagatcc
      721 gaatggcgtc agtttcgata atgaccattt atctgttggt agcagggttc aggtagttaa
      781 aggtgatttc tgtggtgtcg agggcgaact tgccagcgag gccaacaaaa cttatgttgt
      841 tattcgtatt gccggtgtat tgagcgccag cgtcaaagtt cctaaaagtt atttacgtgt
      901 catttaagtt atgaatacac tcacttctca aattgaacaa ttacagagtc tcgcgcacga
      961 gttactctat ttaggtgtgg atggtgctcc tatttatacc gatcatttcc gtcagttgaa
     1021 caaagaagtt ttagaacaat ccgatgcgtt gtatcctcag cgtggtgcta ctcccgaaga
     1081 ggaagcaaac atctgtctgg cattattgat gggttacaat gcaaccatct ataatcaggg
     1141 tgacaaggag gagaaaaagc aagttgttct caatcgttgt tgggacgtgc ttgaccaact
     1201 tcctgctact ttattgaaat gccagttgtt gacttattgt tatggtgagg tgtttgaaga
     1261 ggagttggcg aaagaggcgc atactatcat agaaagttgg aaagataaag aactttctga
     1321 agataaaaaa gaggcaatca ataatttgag aaatttggaa gagaatccat atccttatag
     1381 tgaaatggaa tagaataatt ctattttttt tatttatttg acttatgaaa ggcattgttt
     1441 tagctggggg atctggtaca cgtttatatc ctattactaa aggagtaagc aaacaacttc
     1501 ttccaatttt cgataaaccc atgatttatt accccatctc tgtacttatg ttagctggga
     1561 tacgagaaat actaattatc tctactcctt atgacttacc gggttttcaa cgtttactgg
     1621 gcgatggttc tgattttgga gtacactttg agtatgcaga gcagccttct cctgatggtt
     1681 tagcacaagc atttattatt ggagagaagt ttattggcag tgattctgtt tgtttagttt
     1741 taggagataa tattttttat gggcaaggtt ttacgcgcat gttgcgtgaa gcggttcatt
     1801 ctgctgaatc agaaaacaaa gcaaccgttt ttggttattg ggtaagtgat cctgaacgtt
     1861 atggcgttgc agagtttgat caggagggaa atgtactcag tattgaagaa aagcctcaaa
     1921 tacctaaatc acattatgct gttgtaggac tttatttcta tcctaataaa gtggtggaga
     1981 tagcccaaaa tatcaaacct tctcctcgtg gagaattaga aataactact gttaatcaac
     2041 agtttttgtt agaccaggaa ctgaaggtca aattattagg gcgtggcttt gcttggttgg
     2101 atacaggtac gcatgactcg ctgtccgaag cgagcacttt tatcgaagtg atagaaaaac
     2161 gtcaagggct gaaagttgct tgtttagagg gaattgcttt taggcaaggt tggatttctt
     2221 ccgagaagat gaaagtattg gcacaaccga tgttaaagag ccaatatggg caatatatgt
     2281 tgaagatgat tgatataacg acctctaatg aggaatagat gcctgaatta gatagtaaaa
     2341 aaattaataa tactgacttc ttcaaatata gctaatcctt ataattcttt atttatggat
     2401 aaatagattt ctgagtgttt tttatttagc gaatagaata tttttacatt cagccctgtt
     2461 aaatcgtaat ttgaattgtc gttacattgg atgatttata tgtttacacg aatgattcgt
     2521 tcccttcttt cccgttctcc cctttacgtt ttggctctct atgccagcgt tttgattttt
     2581 ctggtttata cctgtgccta tgcctaccgt aagccattta ccgcagccat ttatgaaggt
     2641 gagattcttt ggggattcga tgtcaaaatc ctgtatgtcc tttctgagat tatcggctac
     2701 gccctttcca agttcatcgg tgttcgtatt ctgccttcga tgaaagcggg tcaccgcatc
     2761 tattatataa tagggctgct tactttttcc gaagttgctt tgttgggttt tgcacttctt
     2821 ccggttcctt tgaaggtgtg ttctattttt ctgagcggtc ttcccctggg tatgatctgg
     2881 ggtgtcattt ttagctatat cgaagggcgt cgtatcagtg aaatattgaa tgtcgggctg
     2941 agcgttgctt tgatagtctc ttccggtttg gtgaaaacct tgggacaatt cgtgatggat
     3001 aatttgcatg tgtcggaata ttggatgcct tttaccaccg gtttgttggt ataccctgtt
     3061 atgttgcttt gcagctggct tctcaatcag attccggctc ccaatgagca agatatcata
     3121 cagcgtacca aacgtgctcc gatggatggt aaagacaggc gtaagttttt gaggcaattc
     3181 ttttggggaa tctgcatgct ggttgttttt tacggtgccc tgaccgtgtt ccgtgagctt
     3241 cgtgactctt tcgctgccga tatttggaag gagctccata ttgagggtgc aatgatcttt
     3301 actcagacag aggttcccat tgccgttttt gtccttgtac tgatgttcct gattgtgttt
     3361 gtccgtaata accgtttggc tcttaatatc atttattgca tcgccgtcac aggcggttcc
     3421 ttaatggttt tttccacatt gctttatgtg tacggttttc tttctcctat ttggtggatg
     3481 attctttccg gtcttggtct gtatatgggg tatattcctt tcacttacct gatagaacgt
     3541 ctgattgctt cccttagggt ggttagtacg gctgttttta ttatttattt ggccgattct
     3601 ttcggctatc tgggcactac gggtgtgttt atggtcaaga acttcgtttc tgctgacatt
     3661 tcctggactg tcatgctgat gcgtactgcc cttttttcag gatttgtttc tgtcctgtcg
     3721 atttttgcga tttactgtta tttcaagcaa caattgaatt cacttatatt aattccctct
     3781 gaaattcatg attaaaacag caatgatcat ggctgccggt cttggtaccc gcttcgggca
     3841 ttataccgag cttgtgccca aaggttttgt ggaggttggt ggaaaaccta tgattatccg
     3901 ctctatcgaa acccttcttt cctgcggtat cgaacgtatc atcctcggta cgggttataa
     3961 aaaagaggct tatgaagccc ttcaggcgga tttcccccag atagagacct gtttcagtcc
     4021 ccgttatgcc gatactaaca gtatgtacac tctgtacaat acgcgtgacg tgataggcga
     4081 tgacagtttc ctgctgcttg aatcggacct tgtttttgaa cgcaaggccc tcctctcgct
     4141 gctggatgac gaattcccgg atgtgatgct tgtctcttct ctgaccaagt tccaggatca
     4201 gtattatgtg gaatatgacc gcaatcacat ccttacttcc tgttcggtag acaaggacgc
     4261 ccttgaggca aaaggcgagc tggtcggtat ccataaactt tccaatactt tctaccgccg
     4321 gatgtgcgcc gattatgcga ccatcctgga gtcccagcct aaactgggtt acgagtacga
     4381 actgctccgc atgtcccgtt ccgtttcccc tgtccgtgtg ctcagggtgg agggtcttaa
     4441 atggtatgaa atcgatgatg aagccgacct ttcgtatgct gaggagcaca tcatccgcta
     4501 ttgttaaaaa caaatctttt ccatatatga acaagaaaat ctatgtcggt atgaccgcgg
     4561 atatcatgca tccggggttg atccatatta ttcatgaagc tactaaatac ggtgatgtca
     4621 tcgtcggtct gctgacggac aaggccatcg ccgaacacaa acgcctccct taccttacct
     4681 atgatcagcg taaggaggtt gttgagaata tcaaaggggt ttgcgaagtt gttccccagg
     4741 aggattggag ctatgttccg aatctggaaa aactcaagcc tgactatatc atccacggcg
     4801 acgactggaa aacgggcccg ttgcgcgagg tgcgcgaaca ggtttttgag gtgatgaacg
     4861 cccagggggg caaggtaatc gagatccctt atacccgggg catcaactcc tcctcgctcg
     4921 acaaggatat caaggcgatc gggactaccc ctgacgtacg tcttaagtcc ctccgccgcc
     4981 tgatccaggc caagcctgtg gtccgtatcc tggaggctca cgacggtctt tgcggtctaa
     5041 ttattgagaa tcttgaagtc cagaagggtg ataagcgcga ggtctttgac ggcatgtggt
     5101 ccagcagcct gaccgactcc acgagtaagg gtaaaccgga tattgaagcg gtcgacctta
     5161 ccacccgttt acaggacctg aacaatatcc ttgaatgtac caccaagcct attatttttg
     5221 atggagacac gggtggcaag atcgaacact ttgtctttac tgtgcgcact ctggaacgtc
     5281 acggcatttc tgcggtaatc attgaagata aagtcggtct gaagaagaat tctctttttg
     5341 gtactgacgc tatccagact caggatagta tcgagggttt ctgcgataag atcagagcag
     5401 ggaagaatgc ccaggtcacc ggtgacttta tgatcattgc gcgtatcgaa agccttattg
     5461 ccggcaaacc catgagtgac gccctcgaac gtgcttatgc ctatgtacag gcgggtgcgg
     5521 acggcatcat gatccacagc aaggataagt cgggtgagga tatccgtgag ttctgccgga
     5581 ctttccgcaa agaatacgcc catgttccca ttgtcgttgt tccgacgact tatgaccatg
     5641 tgcacgaatc cgaacttcac gaatggggtg ccaacgtagt catttacgcc aaccaccttc
     5701 tccgtgccgc ttatccggcc atgatgaacg tggccaggac catccttgaa aacgagcgcg
     5761 ccgaggaggt ccgtccgctt tgtatgccta tcaaggaaat tctggaattg attccgggca
     5821 ctaaataaat cagacggtta tggtaagtgt agaaaagata tacgatacgt tccttcatca
     5881 cggtgttgac ttctttacgg gtgttcccga ttcccttttg aagaatattt gcgcctatat
     5941 cacggatcat gctccgcgtg gtaagcatat catcgccgcc aatgaaggtt ccgcggtggg
     6001 cattgcctgc ggttattata tggcttctgg caacgttcct ttggtttata tgcagaactc
     6061 cggtctggga aatacggtga atcccctctt gtcccttgcg gacgagaaag tgtacagcct
     6121 tcctcttctt ttgctggtcg gttggcgcgg tgagcccggc acgaaagacg aaccccagca
     6181 taagaagcag ggcgaggtta cccttgatct tctgaaagcc atgaaaattc cttttataat
     6241 tctggatacg gatgaagacg aggccctggc gcagatccat gacgcggttg tctcggcgaa
     6301 gagccggtcg attccccatg cgattgtcat ccgtaaagat acgtttggaa aatataaatt
     6361 gcgtcaggaa gccttgaatg actatcctct gtcccgcgag gaggcgctaa tgcaggttgt
     6421 cgaccacctc ggggacaatg acattgtggt ttccaccacg ggcaagctct cccgtgagct
     6481 tttcgagtac cgtgaatcga aagggcaggg gcacgcaagg gatttcctaa ctgtgggttc
     6541 catgggtcac agttcttcca ttgccctggg ggttgccctt gagaagcctg accgccgtgt
     6601 ctactgtctt gacggtgacg gtgcctttat catgcacatg ggtgctgtca gcaatatcgg
     6661 cgatctttct cccaagaatt acaggcacgt ccttttcaat aacggtgccc atgaatctgt
     6721 cggcggtcaa cccacgctcg gtttccgtct ggatatcgcc tcgatagcga aaggttgcgg
     6781 ttatgcgcat gtactgactg cctccgacaa ggaagggctt tcgtgtgcgc ttgagaagct
     6841 gtccggcctg tccggcccgg ttcttcttga aattaaggtc cggatcgatt cacgtgatga
     6901 tctgggtcgc cctacgacta ctccggtaga gaataaagag cattttatgg catttttaaa
     6961 agatacgcat tcatgaatat cgaaagaaag atccttttga atccgggtcc cgccaccacg
     7021 acggatacgg tgaagctggc gcaggttgtt cccgatatct gtccgcgtga gaaagagttt
     7081 gcgggtatga tgaaacagtt acgtgacgac ctggtccgcg ttgcgcatgg agacctctcc
     7141 aaacatacgg ccgtcctgtt ttgcggctcg ggtacgatca atatcgatat ctgcctgaac
     7201 tctcttcttc ctgcggataa gaaagttctg gttgtcaata acggtgccta cagcacccgt
     7261 gcggtggaag tctgcgaata ttacggtctt ccccatatcg atttgaagtt ttccgtttac
     7321 gaacttcccg acctgtcggt aatcgaaaag acgctttccg acaatccgga catcgctttg
     7381 gtctatacca cgcaccatga aacgggtacg ggtatcttga accccatccg tgagatcgga
     7441 aaaatagccc attcgcacgg tgccgttttt gttgtcgaca ccacttcctc cctgggtatg
     7501 attccttttg atatcgggaa ggataatgtt gacttttgta tggcttcggc tcagaaaggc
     7561 cttcaggcca tgacggggct ttcctttatc ataggtgatg aatccctgat acgtgcttca
     7621 agggattatc cgaaacgttc ctactattgt aacctctatt tacaatacga ttactttgag
     7681 aagacgggcg agatgcattt tactcctcct gttcaaaccg tttacgctgc ccgtcaggct
     7741 cttgatgaat attttgcagt aggtgaagag gctaaattcg cccgtcacaa gagggtcttt
     7801 gaagccatcc ataaaggttt gcaagagctt ggtcttcagt atgcgatcaa acgtgaatgg
     7861 cagtcgggtc ttgttgtttc tgtattgtat cctgatgatc ccctctggga ttttgataag
     7921 gttcatgatt actgctatga acgtggcttt acgatctacc cgggtaagat ttccacaacg
     7981 aatacattcc ggctttgtgc gttgggagag attgatgtgg aggatattga gaggttcttt
     8041 gaggtgatga gggaagcaat ttcatttctc aaaatcagtt ttaaaaatat agtttaatta
     8101 tggaagagaa tcaaataact gtaacttcgc ctttattgcc ttctttagac gattttttac
     8161 catatttaga ggatatatgg aagcgtaaat ggattaccaa taatgggtat tatcatcaag
     8221 aattggagaa agctttgtgc caatacttga aagtaccata catctctttg tttacaaatg
     8281 gtactttgcc tttaatttgc tctttacaag ctttgagaat tactggtgaa gttataacaa
     8341 caccatacag ctttgtagca acaacgcatg ctttatggtg gaatggcatt aagcctgtgt
     8401 ttgtggatat tgatcctaaa acttgcaata ttgatccgaa taagattgaa gcagctatta
     8461 ctcctaagac gacagctatt atgccagttc atgtctatgg aaatccttgc gattttattg
     8521 ctatccagga tattgcagat aaatatggac taaaagttat ttatgatgcg gctcatgctt
     8581 ttggagtaga ggtagatgga aaatctcttc tagagtttgg agatatgtcc acactaagtt
     8641 ttcatgcaac taaggtttat aatacaattg aggggggagc attagtttgt catgaccggc
     8701 agattaaaga gagagttgat tttttgaaga attttggttt tgctggtgaa actcaaattg
     8761 tggctcctgg aatcaatggg aaaatagacg agattcgttc tgcatacgga ctacttaatt
     8821 taaaagctgt tgattctgct attgaatacc gacatcaaat atctaaaaga tatcgtgagg
     8881 gattaggagg tattgatggt attagttata tgcctgaaat gcataatata cgtcataatt
     8941 attcttattt tcctatcttt atagattctg taaagtatgg tattactcgt gatgaacttt
     9001 atgaaaaaat gaaacgaaat ggtgtgtgtg gtcggagata tttttatcca ttaataacaa
     9061 acttttcaac gtactgtggg cttgattccg ctcaaataag taatcttcct gttgcaaata
     9121 gaatagctaa ctctgtcatt tgtctgccat tacatactgg cttaacttct attgatgttg
     9181 aacgtataat tgaactttta aaaaaatgac atctttttat tcagaaaaag aattggcaaa
     9241 tataggttta gcagtatacg gcacaaatgt tctgatttct aggaaagtct ctttttattc
     9301 tcccaagaat atatatatag gaaataatgt acggatagac gatttttgta tattgtctgg
     9361 caaaattctc atcggttcca atatacatat atctgcttat gtagctatat atggaggata
     9421 tggagtggtt ttagaggatt atgtgggtat atctcctaga acaacgattt atagtgcaat
     9481 ggatgatttt agtggtgatt atttagtagg tcctattcat gataaaagat taactcatgt
     9541 tattggtgga gaagttcgaa tttgtaagtt tgctcatatt ggcgcaaata ctattgtata
     9601 tcccggatgt tctattggtg aaggaactgt agttggagca atgtctatgg ttcggaaatc
     9661 attggatgct tggggagttt atattggtat acctgctaaa tttctaaaaa atagaaataa
     9721 aaatatgcaa agtttgattt atgggaaaga ataatagaat tccattagtc tctgtttgtt
     9781 gtatgaccta taatcatgat aaatttattt ctcaatgctt agatggtatt attcaacaaa
     9841 aaggtaactt ctctattgaa atattgattc atgatgatgc ttcttctgat catacacgac
     9901 aaataataga attctataga gataagtatc cagatattat aaagcctcta tatcaagaaa
     9961 ttaatcaata ttctcggggg gttaatatat ggaacactta tatgtttcca ttggttaaag
    10021 gagaatatat tgctatatgt gatggggatg actattggat agatcaatat aaattggaga
    10081 aacaaataac tttcttgcaa aaatatgctg actataattt ggtttataca aataaaattg
    10141 ttataaacga attaggaacc agagtaatta ggaatcatcc taaatattta tcgggaaatt
    10201 tgtttgtgaa tttgttaagt ggaggtaatc ctattacaac atctactgta tgtattcgaa
    10261 ctcatgatgc atttgaaata atggatattg taaattctat tccatttaga actgtaatgg
    10321 gagatttaca attatggtta gctttagcca taaaaggaaa gtttaaatat ttggctttta
    10381 aatctactgt ctatagatta tcctctattt ctcttagcca ttctttgagt gtaaatcggg
    10441 aaataagatt ttcaaaacat gaatatttta ttaaaaaata ttttgcagat atatttcagg
    10501 taaatattga taaaaagata ttggaacgaa catattataa gaatacaatt cgtcgattat
    10561 tttcatttag aaaagaaatt tttttgagtt acgtttttaa gtctgtgaaa aaagatccat
    10621 tcttgatttt tgatataaag ataataacat tagtgggggt gaaattatta aaatccattt
    10681 tatgaaagtt ttattggtat ttatagatat gatgagaaca gatttactat ctgtatataa
    10741 tcaaaatgta aaagataaaa caagttttga tttattgtta gaaaaaattg gtggatgtat
    10801 atatactaat tgctatacac cagctcctga tactccacgt agtttagctt gtcttcaaac
    10861 tggattgcat ccatatttca atggatgtaa tacaagaatt aaatggccta aatattatgt
    10921 caaagaggaa atagatacta tttttgatat tctttctaat aataaaattc ttattaatta
    10981 ttatgctcct aaagttgatc ttgatacagg agcgttatct ataaaagaag gaagtgtagt
    11041 gaatctattt actaatattg aaaagtttac ttcaaatatt ttgcagagta cttgcgatga
    11101 aatggacttt ttaacattag atgattatca ttgggccata gatgattatg gcgctaccca
    11161 taaagctgtg aaaataggcc ataatattat ttcaaatgta ttgaatacct tctttagaac
    11221 gcattcttat gatgagttcg atcacatttt tttcttttca gatcatggac ataaattgga
    11281 aaatgagata gctcttatgg aaagtgcttt agaacttttg agagacgata gagctaaaat
    11341 cttaatggta caccgttcga aaagaaatag tggtttgtct attgacaata gattatgttc
    11401 aatacaagat ttatatgcta ctattctatc catttttcat ttgaattatg atcatagaga
    11461 ttcgttatct ttattgtcta cttcaaaatc agatgtacat gatcatctaa taatagagga
    11521 tcatgcagat tttactgttt cgttgaatca ggttataagt gtttggagat atatttcgga
    11581 tgcaggttca ttttatacca atgtttgtaa atatagtaaa gagggatact gtgaactttc
    11641 caattctaca attatatcat taattcaaca aaaatcacct acttatttag attatgaaag
    11701 gcagatgttg atattgaaag aatattctaa aatgtcagag aaatattgta cctattcaaa
    11761 tgggggtaaa cgtttaggaa gaaaaaaaca agttctacta aaggttaaaa ataaatgtta
    11821 tagattcttg ataaaatgtt ttagatgaga agtcgattta tagaattaat aactgtaaca
    11881 gcaagtagca ttatagaatt atctacgact cttttaataa ctaaaattgt tgcagttcaa
    11941 ttatctaagg acggatatgg attttactct ctcatattat ctgtagtggc tcttttctct
    12001 ttattaccat tttcgtcatt gcatgttggc attgaacgtt atattataga atataaaaat
    12061 aggaacaagt ttgctagatt ttatgcttca atatatttaa ttcatattgt cttttttgtc
    12121 ttatatttgt tgcttttgtt gcctctaagt ccctttttaa caggggaatg gaatgagatt
    12181 tgggagattg tatatttatt ctttattaca aaagtttata aacagtttgt tttctgcatt
    12241 ttgaatatta atcgtaaaag aatttctata ttatatgcga gaataattga tttaagtatt
    12301 caacttatat tattgtattt ttttcttata aaagggttgt ctattcaaga aatattatat
    12361 gcttcaatag tgagtagttt actaactatt ggatatctag tttatattga ttcgcataat
    12421 atctatttaa aaaaggttag agttagtgat tttttattgg tattgaaaga tgtgtttagc
    12481 tattcatatc ctttaattat atggggaatt tttgcttgga ttcaatcaat gattggacgg
    12541 tggtttgttg atttttattt aacaaaatct gatgttgcta acttcagtat gatattttca
    12601 ttatctctgt tgccatctac tgcaataagt gctattattg gtagttattt tgttccaata
    12661 gcatattcaa atgaagcgaa atctactggg tttattggta ggtatatcaa taaaattcta
    12721 tttgctgttt cacttgtgtg gcttgtgatt atattgactt tcttattttt gggtgaatat
    12781 attattgtac tttttttaga cgtaaaatat ttaaatatat attggtactt gttttggggg
    12841 ttattaggga ctggcgtcta tacattagga cagttgtctg tgtatgaatt atactattat
    12901 aaaaaagtga aatacttgtt gctacctaat ctactaccgg gccttttgac aattgttgga
    12961 tgtatgatta ttgttccttt ttggggattt gatggagctg tatatgtgaa tgtatttact
    13021 tatgtaatat cagggatttt aactttatat tgtactttat attatggtcg taaaattaaa
    13081 ataatttatg gtaattgcta aattatttgg aggattagga aatcaaatgt ttatttatgc
    13141 agctgcaaaa ggtattgcac aaatttcaaa ccaaaaatta acatttgata tttataccgg
    13201 ctttgaagat gattctaggt ttcggagagt ttatgaatta aaacaattta atttgtctgt
    13261 acaggaatct aggaggtgga tgtcttttcg atatccgcta gggagaatac tgagaaaaat
    13321 tagccgaaaa atcggatttt gtattccttt ggttaatttt aaatttatag ttgaaaagaa
    13381 gccatatcat tttcaaaatg aaataatgag gatagcttcc ttttcttcaa tttacttaga
    13441 agggtatttt caatcatata aatacttctc aaaaatagaa gcacaaataa gagaagattt
    13501 taaatttact aaagaagtta ttggttcagt tgagaaagag gcttctttta taaccaactc
    13561 acgatataca ccggtggcta taggcgtacg ccgttattcc gagatgaaag gggaatttgg
    13621 tgaattagct gtggtggaac atgattatta tgatgcggcc ataaaatata tagcaaataa
    13681 agttcctaat ttaattttta ttgttttttc tgaagatatt gattgggtaa aaaaaaattt
    13741 gaagttagat tatccagtgt attttgttac ttctaaaaag ggagagttag cagctattca
    13801 agatatgtat ttaatgtctt tatgtaatca tcatataatt tcgaatagta gtttttattg
    13861 gtggggtgca tatttggctt ctacaaacaa tcacattgtt atagctccat ctgttttctt
    13921 aaataaagac tgcactccta tagattgggt gattatttaa ttgtaagcta tttacttgtc
    13981 ttttattaat agaaccactg cacttttaat ttttttgatt gattttgttg tttgttctat
    14041 ttgacaagaa ttaattttta tgttttttat ttatcttaaa tttcacatta tccattcttt
    14101 cttttgtcac ctcaatttaa gataaaatgt aattacgatt tgtaactgtg agttctattt
    14161 atgattagtt tgtgtttttt gggaaagagt taatagagag ctacatctt
//
