LOCUS       gnlProkkaLHDDDFDO_469  12632 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LHDDDFDO_469.
ACCESSION   gnlProkkaLHDDDFDO_469
VERSION     gnlProkkaLHDDDFDO_469
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 16:38:15
            Original ID  :: gnl|Prokka|LHDDDFDO_469
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12632
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..12632
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[3318:7632]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3319..7632
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..12632
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..12632
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             3..2096
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Poly_export"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg469_1"
                     /sec_met_domain="Poly_export (E-value: 3e-18, bitscore:
                     55.9, seeds: 183, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQDLARGDQVFGRNIFNTRNLTFEPSVNLATPANYRLGPGDEVI
                     IDIWGASQNTIRQQISPEGTINIQKIGPVNLSGMTVSAANDYLKGALNKIYNGLNNTTD
                     PTSDIRLTLGNIRTIQINVMGEVVQPGTYALSSFSTVFHALYRAGGVSDIGSLRNVQLV
                     RNGKNIATIDVYEFIMKGNTQDDIRLQEGDVVIVPAYDVLVKISGKVKRPMRFEMKKEE
                     NLATLIKYAGGFEADAYTRSLRVVRQNGEEYEVNTVKDIDYSIYKMRNGDVVTAEAILN
                     RFTNKLEIRGAVYRPGIYQLSGKLNTIRELVHEAQGLTGDAFLNRAVLYRQREDLTSEV
                     VQIDIKSIMDGTSPNLALMKNDILYIPSIHDLEDRGNVTVHGEVAHPDSYPYADNMTLE
                     DLIIQAGGLKEAASTVRIDVSRRIKNPRSIADNDTIGQMYTFSLKDGFVIDGQPGFILQ
                     PYDEVYVRRSPGYQAQQNVAIEGEILFGGNYAMTSREERLSDLVNKAGGPTNYAYLRGA
                     KLTRVANASEKKRMGDVIRLMSRQLGEAMIDSLGIRVEDTFTVGIDLEKALSNPKSNAD
                     LVLREGDVISIPKNTNTVTINGAVMVPNTVSYMKGKDVDYYLNQAGGCSDNARKSKKFI
                     VYMNGQVTKVKGSGKKQIEPGCEIIVPGKAKKKGNIANILGYATSFSSLGMMIASIANL
                     IKK"
     CDS             2107..3255
                     /locus_tag="ctg469_2"
                     /transl_table=11
                     /translation="MIEKQSYQEPFSEVDKRKDDEMEIDLMAIFYKIITVRGVLCKAAG
                     VGVIIAIVVALSIPKQYTVKVTLSPEMGNAKGSNGLAGLAASFLGDGALIGESTDALNA
                     SLSADIVSSTPFLLELLEMEVSVSKKDDKMTLGSYLDEESSPWWDYLIGFPGIVIGGVK
                     SLFGEDTVPASGGRQGTIELTKEVNEKINFLKKKISASIDKKTAITSITVTLQNPQITA
                     VIADSVVHKLQEYIIGYRTSKVKEDCAYLERLFKERQQEYYAAQRKYADYVDAHDNVVL
                     QSVRAEQERLQNDMSLAYQIYSQVANQLQVSRAKVQEEKPVFAVVEPAVVPLKPSGMGL
                     KIYILLFVFLSVSGTLVWVFFGKSLLASLRKELKNKEIEQDK"
     CDS             3319..4038
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 54.5; E-value:
                     1.6e-16)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg469_3"
                     /sec_met_domain="NTP_transf_3 (E-value: 2.3e-16, bitscore:
                     50.8, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRTAMIMAAGMGTRFGQYTELIPKGFVKVGGIPMIVRSIDTLLS
                     CDIERIVIGTGYKQEVYEELKTDYPMLETCFSPRYAETNSMYTLYNTREILGNDDFLLL
                     ESDLIFEKQAIMSLLECPAADAMLITPVTKFQDQYYVEHDDNFRLSSCSVNKNKLNAKG
                     ELVGIHKLSGSFYKIMCADYASIVDEQPNLGYEYELLRISCSQSPVYVHKVEGLKWYEI
                     DDISDLEYAEKYIVPYC"
     CDS             4059..5360
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 208.9;
                     E-value: 1.5e-63)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg469_4"
                     /sec_met_domain="CTP_transf_like (E-value: 2.7e-13,
                     bitscore: 40.6, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 2.8e-121, bitscore:
                     394.2, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 3.2e-63, bitscore:
                     203.6, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKTVYIGMTADIMHPGLIRIINEATKYGDVIIGLLTDKAIAEHK
                     RLPYLTYEQRKEVVQNIKGVCKVVPQEEWSYVENLKRIKPDYIIHGDDWKTGPLREERV
                     RVFEVMNEQGGKVIEIPYTLGINSSSLDKDIKAIGTTPDVRLKSLRRLINAKPVVRILE
                     AHDGLCGLIIENQEILKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAIIIEDKVGLKKNSLFGTDVIQTQDT
                     IEGFCNKIKAGKASQITDDFMIIARIESLIAGKPVSDALERAFAYVQAGADGIMIHSKN
                     KSGEDIKEFCLAFRKQYAHVPIVVVPTTYDHIYESELCDWGVNIIIYANHMLRAAYPAM
                     MNVAKIILENERALEVRELCMPIKEILELIPGTK"
     CDS             5377..6501
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 58.1;
                     E-value: 1.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg469_5"
                     /sec_met_domain="TPP_enzyme_N (E-value: 1.5e-20, bitscore:
                     63.7, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.3e-20, bitscore:
                     64.0, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSVEKVYETFLSHDVDFFTGVPDSLLKNICAYITDHTSREKHII
                     AANEGAAVGIASGYYMASGKVPVVYMQNSGLGNTVNPLLSLADEQVYSFPMLLMIGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMRIPYIILDSNENDAFIQLHDIIQSAKTKNIPHAII
                     IRKDVFGKYKLQKEFGCNYPLSREDALQIVVDYLPENSVVVSTTGKLSRELFEYREMKE
                     QGHEHDFLTVGSMGHSSSISLGIAIAKQDRPVYCLDGDGAFIMHLGAISNIGDLSPKNY
                     YHILFNNGAHESVGGQPTLGFSLDIPAIVRGSGYKHTYTVCTKVEIEEAMKQLPKLCGP
                     VLLEIKVKIDSRENLGRPTTTPIENKEHFMDFLK"
     CDS             6511..7632
                     /NRPS_PKS="Domain: Aminotran_5 (30-366). E-value: 2.9e-34.
                     Score: 110.4. Matches aSDomain:
                     nrpspksdomains_ctg469_6_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 76.3; E-value:
                     3.6e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg469_6"
                     /sec_met_domain="Aminotran_5 (E-value: 2.1e-31, bitscore:
                     99.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIERKILLNPGPATTTDSVKMAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGNLEKHTAVLFCGSGTINIDICLNSLLPEDKRVLIVNNGAYSTRAVEVCQYY
                     GLPHINLEFSVYERPDLSVVENVLQENPDIAMVYTTHHETGTGILNPIREMGAIAHKYQ
                     AKFVVDTTSSLGMIPFDIEKDNVDFCMASAQKGLQAMTGLSFIIGNEEQIKFSKKYPKR
                     SYYCNLYLQYENFERTGEMHFTPPVQTIYATRQALNEYFEVGEEAKFARHKRVFEAIHS
                     GINEIGLKSVIKREWQSGLVVSVQYPEDPNWDFEKIHDYCYERGFTIYPGKISTEDTFR
                     ICALGEIEVDDIENFFKVLKAALNHYNVTLPMS"
     aSDomain        6601..7608
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg469_6_Aminotran_5.1"
                     /evalue="2.90E-34"
                     /label="ctg469_6_Aminotran_5.1"
                     /locus_tag="ctg469_6"
                     /protein_end="366"
                     /protein_start="30"
                     /score="110.4"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGNLEKHTAVLFCGSGTINIDICL
                     NSLLPEDKRVLIVNNGAYSTRAVEVCQYYGLPHINLEFSVYERPDLSVVENVLQENPDI
                     AMVYTTHHETGTGILNPIREMGAIAHKYQAKFVVDTTSSLGMIPFDIEKDNVDFCMASA
                     QKGLQAMTGLSFIIGNEEQIKFSKKYPKRSYYCNLYLQYENFERTGEMHFTPPVQTIYA
                     TRQALNEYFEVGEEAKFARHKRVFEAIHSGINEIGLKSVIKREWQSGLVVSVQYPEDPN
                     WDFEKIHDYCYERGFTIYPGKISTEDTFRICALGEIEVDDIENFFKVLKAALNHY"
     CDS             7629..7772
                     /locus_tag="ctg469_7"
                     /transl_table=11
                     /translation="MIAEILLKIRHFYFSLRGIHFHPESVISGVPIRSFRLVGKYGNIN
                     LR"
     CDS             7975..8196
                     /locus_tag="ctg469_8"
                     /transl_table=11
                     /translation="MENLKQKTVSGIMWSAIERFSLQGVQFIIQIILARLLLPSDYGMI
                     GMLAIFLQVAQVFIDSXXXXKCFNPEKR"
     CDS             8303..9463
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg469_9"
                     /sec_met_domain="Polysacc_synt (E-value: 7.8e-15, bitscore:
                     45.2, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTLVLVIRFIALSLIINALSAIHRTKLIISVNFKTQSKISLGAA
                     FISGVIGIWMAYVGCGVWALVWQTLLNSIILTILFYCLVHWKPLHTFSKSSFERLFNFG
                     SKLLISSLINTVYRNLYTIVIGKKFSVVELGYYTRAEQFAIFPSNNLNALVSRVAYPIL
                     SSIQDDDKCLTNAYRNYIRLSSYIIFPLMVGLLVLANPIISLLLTDKWSGVVILLQILC
                     LDWMFDHLSQINLNLLWVKGRSDLSLRLEIIKKTIAIFILFVSIPFGLEVMCWGRVIYS
                     LIATYLNTYYTNSLIDLTLKLQVKDVFPSLILSFIMGGIVFICISFFETDIVKIVIGCI
                     TGMSFYILLSFLFKLDSFFCILTLIIKTKNIDENIKKDFSSNSKDN"
     CDS             9417..10676
                     /locus_tag="ctg469_10"
                     /transl_table=11
                     /translation="MKILKKIFQVIVKIINHCVPVDSRSLYFIPHPNCRTDKYDIINYS
                     SDNVLVLLNYLLKKDSAEYYKIYVEIYDSSRISEYQEYIYGINPNIRCTFLCACVGRFF
                     FNKVSFKDMLYAFFCFCRASKCFTATFYYDFSFKKRSQQIICLGYYTPFKDDYHMGDHS
                     YDKFRNTTCNSFDYSIATSCLSARIISIDCGISYDKFKVLGFPRNDLLISKNNCNVIKR
                     EISKFAGYDVTKYIVYTPTFRDYETVTEGNLRSILGYVDCDLLKLSKILLKFNAALILK
                     LHPLQNKTVLKKDLPKGILVFEQTYKYGLYDLLSFSDGMITDYTSTYFDFLLVNKPVIF
                     NFYDIEEYRRVRGFSFEPIEFFCAGDIVYNYNELIDAVMGLLAGKDIHAEHRRHISLLM
                     NQFQDDNSTKRICDIFLNEI"
     CDS             10685..11434
                     /locus_tag="ctg469_11"
                     /transl_table=11
                     /translation="MNYAFVVQARLGSTRLPGKILKPFYGNQSILDLMVHKLSAISNIP
                     VIIATTNSVINEPIEKKALALGVKCFRGEENDVLKRFIDVAEYFDIQGIFRICSDNPFL
                     DVHAARQLVEIAMKSCNDYISFDIDGTPSIKTHFGFWGEFVTLDALKRVIGFTDDLLYR
                     EHVTNYIYSHPELFNIQWISGSPVVSKHHNIRLTIDTLEDFSVAQRIYRDLQEKRVEIS
                     IEVIVDYVRRHAEYIQLMKQQIKKNSK"
     CDS             11434..12474
                     /locus_tag="ctg469_12"
                     /transl_table=11
                     /translation="MKNTYIIGEIGQNHNGSVDLAKLIIELISRPVHEDVFGMDFMPMN
                     AVKMTKRDLSEELAVSQMNQIYDNPNSFGRTYGEHRAFLELDDQAHFEIYKYAKSLGLD
                     FIETLCAKGCLSLLKLFTPDRLKVASRDLTNLPLLEALAETHIPIILSTGMAGQRELDN
                     ALDVITRYHSNIAILHCVSQYPTHPNNLNLRTITYLKKHYNKFEIGFSDHTIGISAATA
                     AVAMGAEIIEKHVTIDRHMKGTDQLGSVGPDGVNRMIRDIRIAECWLGTEDLYIEKGVE
                     KSKIKLERSIATRKYIPAGSIIQESDIHLLSPGDGYKWIDKKNVIGHFAKQDISANEII
                     YPDFIE"
ORIGIN
        1 cgactactca ggaccttgcc cgtggtgatc aggtcttcgg acgtaacatc ttcaataccc
       61 gcaacctgac gttcgagcct agtgtcaacc ttgccactcc tgccaattac cgtctgggtc
      121 ccggtgatga agtcatcatc gatatctggg gtgctagcca gaacacgatc cgccagcaga
      181 tctctcctga aggcaccatc aatattcaga agatcggtcc tgtcaacctt agcggtatga
      241 cagtctccgc cgccaacgac tatctgaagg gcgccctgaa taagatctat aacggtctga
      301 acaatactac cgaccctact tccgacattc gtctgacgtt aggtaatatc cgtactattc
      361 agatcaatgt gatgggagag gtcgtgcagc cgggtaccta cgccctttct tccttctcta
      421 ccgtcttcca cgcgctttac cgtgcaggcg gcgtgagtga tatcggcagc ctccgaaacg
      481 ttcagttggt acgcaacgga aagaatatcg ccaccatcga cgtctacgaa tttatcatga
      541 aaggcaacac acaggatgac atccgccttc aggaaggtga cgtcgtgatt gttcccgctt
      601 atgatgtatt ggtaaagatc agcggcaagg taaaacgtcc tatgcgtttt gaaatgaaga
      661 aagaagagaa ccttgccact ttaatcaaat atgccggcgg ctttgaagcc gatgcctaca
      721 cccgttcatt gcgtgtcgtc cgtcagaatg gtgaggaata cgaagtcaac acagtcaaag
      781 atatagatta cagcatttat aaaatgcgta acggcgatgt tgtaacagcc gaagccatcc
      841 ttaaccgctt cacaaacaaa ctggaaatcc gtggcgctgt ctatcgtccc ggtatctacc
      901 agctcagcgg taaactgaac acgatccgcg aacttgtcca tgaagctcag ggtctgaccg
      961 gtgacgcttt cctgaatcgt gccgtccttt atcgccagcg tgaggatttg accagtgaag
     1021 ttgttcagat agacatcaag tctatcatgg acggtacttc cccgaacctt gccctgatga
     1081 agaatgacat cctttacatt ccgagcattc acgatctgga agatcgtggc aatgtcacag
     1141 ttcacggtga agtcgcgcac cctgattcct atccctatgc tgataacatg acacttgagg
     1201 acctgatcat ccaggccggt ggcttgaaag aagctgcttc tactgtccgc attgatgtgt
     1261 cgagacgaat caagaatccc cgtagtatcg cggataatga tactatcggt cagatgtata
     1321 ccttctcttt aaaggatggt tttgtcatcg acggtcagcc cggttttatc cttcagcctt
     1381 atgacgaagt ttatgtccgt cgcagtccgg gctatcaggc acaacagaat gtagccattg
     1441 aaggtgaaat ccttttcgga ggaaattacg ccatgaccag ccgtgaggaa cgtctttccg
     1501 atttggtgaa taaagccggt ggtccgacca attatgccta tctgcgtggt gcaaaactga
     1561 cccgtgtggc taacgctagt gagaagaaac gtatgggtga tgtaattcgc cttatgagca
     1621 ggcaattggg cgaggccatg attgactctt tgggtattcg tgtcgaagat acctttacgg
     1681 ttggaattga tttggaaaaa gccttgagca atcctaaaag taatgccgat cttgtcttac
     1741 gtgaaggaga tgtgatctcc atacctaaga atactaatac tgtaactatc aatggtgctg
     1801 tcatggttcc gaatacggtt tcttatatga agggaaagga tgtggattat tacctgaatc
     1861 aggccggtgg ctgttctgat aatgcaagga agagtaagaa gttcattgtt tacatgaatg
     1921 gtcaggtgac gaaggttaag ggtagtggta agaaacagat tgagccgggg tgtgagatta
     1981 ttgtgcctgg taaggctaag aagaaaggga acatagctaa tattttaggt tatgctactt
     2041 ccttcagttc tttgggaatg atgattgctt ctattgctaa tttgataaag aaataaatga
     2101 cctattatga ttgaaaagca atcttatcaa gaaccgtttt ctgaagtgga taaaaggaaa
     2161 gatgatgaaa tggaaataga cttgatggct attttttata agattattac tgttcgtgga
     2221 gtactatgca aagctgctgg agtgggagta ataattgcta tagtggtggc attgagcatt
     2281 cccaagcaat atacagtaaa ggttaccctt tctcctgaaa tgggtaatgc gaagggaagt
     2341 aatggtttag ccggacttgc tgcctcgttt ttgggagatg gggctctaat aggggaaagc
     2401 acagatgcct tgaacgcttc tctttcagca gatattgttt cttcaactcc ttttttattg
     2461 gaacttttag aaatggaagt ctccgtttct aaaaaggatg ataaaatgac tttggggtct
     2521 tatctcgatg aagagtcatc tccttggtgg gattatctta taggttttcc gggcatagta
     2581 ataggaggag ttaaatcatt attcggagaa gatacggttc ctgccagtgg agggcggcaa
     2641 ggaactatcg aattgacgaa agaagtaaat gagaagatta attttcttaa aaagaagatt
     2701 agtgcatcca ttgataagaa aaccgccata acgagcatta cggtcacctt acaaaatccg
     2761 cagatcactg cggttatagc agattctgtc gttcataaat tacaggaata tattattggg
     2821 tatcgaactt caaaagtaaa agaagactgt gcgtatttgg aacggttgtt taaagaacgt
     2881 cagcaggaat attatgctgc tcaaagaaaa tatgcggatt atgtagacgc ccatgataat
     2941 gtggttcttc aaagtgtacg tgctgagcag gagcgtttac agaacgacat gagtttggct
     3001 tatcagatat atagtcaggt tgctaatcag ttgcaggtat ccagagccaa agtacaggag
     3061 gaaaagccag tctttgcagt agttgagcct gccgttgtac ctttgaaacc ttcaggtatg
     3121 ggactcaaaa tttatatttt attatttgtg ttcctgtccg tatctggtac tctcgtgtgg
     3181 gtgttttttg gcaaaagtct gctggctagc ttgagaaaag aactgaaaaa taaggagata
     3241 gaacaggata aatgatgcta aaacgtcgtt tacaacattc ttacatcaat taaattcact
     3301 tactatatta ttaaacgaat gatacgtaca gctatgataa tggctgcggg catgggaacc
     3361 cgttttggcc aatatacaga attgatacct aaagggttcg tcaaagtggg aggtattcca
     3421 atgatagttc gttctattga tactttgtta tcatgtgaca ttgagcgaat tgtaatcggt
     3481 acaggatata aacaagaagt ttatgaagaa ttgaagacag actatcctat gcttgaaacc
     3541 tgttttagtc ctcgttatgc tgaaacaaat agtatgtata ctttatataa tacgcgggag
     3601 attctgggca atgatgattt tttattgtta gagtcagatt tgatttttga gaaacaagca
     3661 ataatgagct tattggagtg tcctgcggca gatgcaatgc taataactcc agtgacaaag
     3721 tttcaagatc agtattatgt ggaacatgat gataattttc gcctttcttc atgttctgtc
     3781 aataagaata aactaaacgc taaaggcgaa ttggtcggca tccataaact atccggttcc
     3841 ttttacaaaa taatgtgtgc tgattatgcg agtattgttg atgaacaacc caatttggga
     3901 tatgagtatg aattactacg aatatcttgt tctcaatctc ctgtttatgt gcataaagta
     3961 gaagggctga aatggtatga aatcgatgac atatcagatt tggaatatgc agagaaatat
     4021 attgttccgt attgttaatt aaaataatat atcaaattat gagtaagaca gtttatattg
     4081 ggatgactgc ggatattatg catccaggac ttatccgcat tattaatgag gctactaaat
     4141 acggtgatgt tataattggt cttcttacag ataaagctat tgcggagcat aaacgccttc
     4201 cttatctgac ctatgaacaa cgaaaagagg tagttcagaa tattaaaggc gtatgtaaag
     4261 ttgttcctca agaagaatgg agctatgttg agaatctcaa acgcattaaa cctgactata
     4321 taattcatgg tgacgactgg aaaaccgggc ctttacgtga agaacgtgtg cgcgtatttg
     4381 aagtgatgaa tgaacaggga ggaaaggtta tcgaaattcc atatactttg ggaattaatt
     4441 catcttcttt ggataaagat attaaggcta taggtacaac ccctgatgta cgtttaaaat
     4501 cacttcgtcg tttgataaat gccaaacccg tagttcgtat tttggaagct catgatggac
     4561 tatgtggctt gataattgaa aatcaggaaa tattaaaagg ggataagagg gaagtatttg
     4621 acggaatgtg gtctagtagc cttacggatt caaccagtaa aggtaaacct gatattgagg
     4681 cggttgattt aactacccga ttgcaagatt tgaataatat tctggagtgt actactaaac
     4741 ctattatttt tgatggagat acaggtggta aaattgaaca tttcgtattt acagtacgca
     4801 ctttagaacg tcatggaatt tcagctataa taattgaaga taaagttgga ttaaaaaaga
     4861 attcattgtt tggtacagat gttattcaaa ctcaagatac aatcgaaggt ttctgtaata
     4921 agatcaaagc aggaaaggca tctcagatta ctgatgattt tatgattatc gctcgtattg
     4981 agagccttat agcgggaaag ccggtaagtg atgctttaga gagagctttt gcatatgtcc
     5041 aagcgggagc ggacggtatt atgatacata gcaaaaataa gtcgggtgaa gatataaaag
     5101 aattttgttt ggcgtttcga aaacagtatg ctcatgtacc tatcgtggtt gtgcctacta
     5161 catatgatca tatttatgag tctgaattgt gtgattgggg tgtgaatata attatttatg
     5221 ctaatcatat gcttcgtgcc gcttatcctg ctatgatgaa tgtcgcaaag ataatattag
     5281 agaatgaacg tgctttagag gtgcgcgaac tgtgtatgcc cataaaagaa atattggaac
     5341 tgattccagg tactaaataa aaagaggaat atcattatgt taagcgtaga aaaagtttat
     5401 gaaacgtttc tgagtcatga cgttgatttt tttacaggag ttccagactc attactaaaa
     5461 aatatttgtg cctatatcac agaccatact tcacgtgaga aacatatcat agctgctaat
     5521 gaaggtgcgg ctgtaggaat tgcttcaggt tattatatgg catcaggtaa ggttcctgtg
     5581 gtttatatgc aaaattccgg cttgggtaat actgttaatc ctctgctgtc tcttgcagat
     5641 gaacaagtat acagttttcc aatgcttttg atgattggct ggcgtggtga accgggaaca
     5701 aaagatgaac ctcagcataa aaaacagggt gaggtaactt tagatttgtt gaaagcaatg
     5761 cggattcctt atataattct tgattcaaat gagaatgatg catttattca gctccatgat
     5821 atcatacaat cagcaaaaac taagaatatt cctcatgcaa taattattcg gaaagatgtt
     5881 tttggcaaat ataaacttca aaaagagttc ggatgtaatt atcctttatc tcgtgaggat
     5941 gctttgcaaa tagttgtgga ttatctgcct gaaaattcag tggtcgtttc tactactggc
     6001 aagctttctc gtgagttatt tgaatatcga gaaatgaaag agcaaggtca tgaacatgat
     6061 tttctcacgg taggctctat gggacatagt tcatcaatct ctttgggtat tgctatagca
     6121 aagcaagata gacctgttta ttgcttggat ggtgatggag catttattat gcatttaggg
     6181 gcaattagta acattggcga tttatctcct aaaaactatt atcatatttt gtttaacaat
     6241 ggtgctcacg agtctgtagg aggacagcct actttaggat ttagtttaga tattccggct
     6301 attgtccggg ggagcggata taagcatact tatactgttt gtacaaaagt tgaaattgaa
     6361 gaagcaatga agcagcttcc aaagttatgt ggtcctgttt tgttggaaat aaaggttaaa
     6421 atagattctc gggagaattt gggacgtccc acgactactc ctatagagaa taaggagcat
     6481 tttatggatt ttttaaaata aatatattcg atgaaaatag aaagaaaaat attattaaat
     6541 cctgggcctg ccactactac tgacagtgta aaaatggcac aagttgttcc ggatatttgt
     6601 cctcgcgaga aagaattcgc aggtatgatg aaacaacttc gggacgattt ggtcagagtt
     6661 gctcatggga atttggaaaa acataccgct gtattatttt gtggctcagg aaccatcaat
     6721 attgatatat gtctaaattc tcttcttcct gaagacaaaa gggtgcttat agtgaataat
     6781 ggggcatata gtacccgtgc tgtagaagtg tgtcaatatt atggtttgcc tcatattaat
     6841 ttggaatttt ccgtttacga acgtcctgat ttatcggtgg tggaaaatgt gttgcaagaa
     6901 aatcctgata tagctatggt ttatactact catcacgaga cgggtacagg aattctgaac
     6961 cctattcgcg agatgggcgc tatagctcat aaatatcagg ctaaatttgt cgtcgatact
     7021 acttcttctt tgggcatgat tccttttgat atagagaaag ataatgtgga tttttgtatg
     7081 gcatctgctc aaaaaggact tcaagcgatg acaggtcttt cttttattat aggcaatgag
     7141 gaacagatta agttttcaaa aaaatatccc aagcgttctt actattgtaa tctttatttg
     7201 cagtatgaga attttgagag aacaggtgaa atgcatttta ctccaccggt acaaactatt
     7261 tacgccactc gtcaggcatt aaatgaatat tttgaagtgg gtgaagaagc taagtttgcc
     7321 cgtcacaaaa gagtttttga ggctattcat tcaggtataa atgaaattgg tcttaaaagt
     7381 gtaataaagc gtgaatggca gtcaggactg gttgtctcag ttcaataccc agaagatccc
     7441 aactgggatt ttgagaaaat acatgactat tgctatgagc gtggatttac gatttatcca
     7501 ggtaagatat caactgagga tacttttaga atctgtgctt taggagagat agaagtagat
     7561 gatattgaga acttctttaa agtactaaaa gctgcattaa atcattataa cgtcacatta
     7621 ccgatgtcat gattgcagaa atattattga agattcgtca tttttatttt tcccttcgtg
     7681 gtatacactt ccatccggaa agtgttattt ctggagtgcc tattcgtagc tttcgcttag
     7741 taggcaaata tggcaatatt aatttgaggt gatgcattag gagttcttta ttcttataaa
     7801 aaggcatcgg tagttatagg agataatgtt tgtgtagggt aatttgcttt tagtgggaga
     7861 gaccatatta gaattgcggt aaggggcgaa gaagataata accaattagt gagaatattg
     7921 gaaaaatata attgatatcg gtaaatgtag ttgtttttat actgtattta agtaatggaa
     7981 aatctaaagc agaaaactgt atcaggaata atgtggagcg ctatagaacg tttctcttta
     8041 caaggagtac agtttataat tcaaataata ttagctcgtt tgttactacc atcggattat
     8101 ggtatgatcg ggatgttagc tatttttctt caagtcgctc aagtatttat agatagcnnn
     8161 nnnnnnnaca aatgctttaa tccagaaaaa agatagaact gaagaggatt ttgccactgt
     8221 cttttatttt aatattcttg tcgcagtatt attctatggt atattgtttt tttcggcttc
     8281 tttgattgca gacttttata atatgccgac tttagttctt gtaattcgtt ttatagcatt
     8341 gtcgttgata ataaatgctt tatcggctat tcacagaacg aaattaatta tttctgtaaa
     8401 ctttaaaact caatctaaaa tttcattagg tgcagctttt atatcaggcg tgattggaat
     8461 atggatggct tatgtcggtt gcggggtttg ggctttagtt tggcaaactc tcttaaatag
     8521 tataattttg acaattttgt tttactgttt agttcattgg aaacctttac atactttctc
     8581 caaaagttct tttgaaagat tattcaattt cggatctaaa ttattgatct caagtcttat
     8641 caatacagta tatcgtaatc tgtataccat tgtaatagga aaaaagtttt ctgttgtaga
     8701 gcttggttac tatacaagag cagaacaatt tgctattttt ccttccaata atttaaatgc
     8761 tttagtttct agagtagcgt atcctatttt aagttccatt caagatgatg ataaatgttt
     8821 aacaaacgca tatcgaaatt atatcagatt atcctcatat ataatttttc ctttaatggt
     8881 aggtttactt gttttggcta atcctattat tagtctattg ttaacagata aatggtctgg
     8941 ggttgttatt cttttacaga tattatgttt ggattggatg tttgatcatt tgagccaaat
     9001 taatttgaat ttattatggg ttaaaggaag atctgatctg tcgttacgtc ttgaaataat
     9061 aaaaaagacg attgctatat tcattctttt tgtgtcaatt ccatttgggc ttgaggtgat
     9121 gtgttggggt agagttattt actctctgat agcgacatat ttgaatacat attatactaa
     9181 ttctcttatt gatttgacct taaagttgca agttaaagat gtatttccaa gtttaatact
     9241 ctctttcata atggggggga tcgtttttat atgtatttct ttctttgaaa ctgatatagt
     9301 aaaaattgtg attggatgta ttactgggat gtccttctat attttacttt cttttctttt
     9361 taagctagat tctttttttt gtattttaac attgatcatt aaaactaaaa acatagatga
     9421 aaatattaaa aaagattttt caagtaatag taaagataat taaccattgt gtgccagttg
     9481 atagtagatc attgtatttt attcctcatc ctaattgtag aacagataaa tatgatatta
     9541 taaattattc gtcagataat gttctcgttc tgttaaatta tttgctaaaa aaagatagtg
     9601 ctgaatatta taaaatatat gttgaaattt atgattcttc tcgtatttca gaatatcaag
     9661 aatatattta tggcataaat cctaatatta ggtgtacttt tttatgtgca tgtgttggac
     9721 gttttttctt taataaagta tcttttaagg atatgttgta tgcctttttt tgtttttgta
     9781 gagcaagtaa atgttttaca gctacttttt attatgattt ttcatttaaa aaacgctctc
     9841 aacagattat ttgtttgggg tattatactc cattcaaaga tgattatcat atgggagatc
     9901 attcttatga taaatttcgt aatacaacat gtaactcatt tgattactct attgctactt
     9961 cttgtttaag tgctagaata atttcaatag attgtggaat ctcatatgat aaattcaaag
    10021 tcttaggatt tcccagaaat gatttgttga tatcgaaaaa taattgcaat gttattaaaa
    10081 gagaaatttc gaaatttgcg ggatatgatg taactaaata tatagtttat actccaactt
    10141 ttcgtgatta tgagactgtt actgaaggta atttgcgttc aatactcgga tatgttgatt
    10201 gtgatttgtt gaagttatcg aaaatactat tgaagtttaa tgcggcttta attcttaaat
    10261 tacatccttt gcagaataaa acagtcttaa aaaaagattt acccaaaggt atacttgttt
    10321 ttgaacaaac atataaatat ggattgtatg atcttttgtc attttcagat ggaatgatta
    10381 ctgattatac ttctacctat tttgattttt tattggtaaa taagccagtc atatttaatt
    10441 tctacgatat tgaagaatat cgtagagtaa gaggtttctc atttgagcca atagaatttt
    10501 tttgtgcagg tgatattgtt tacaattata atgaattgat agatgctgtg atgggacttc
    10561 tggctgggaa agatattcat gctgaacatc gacgtcatat ttctttattg atgaatcaat
    10621 ttcaagatga taattcaaca aagagaatat gtgacatatt tttaaatgaa atataataaa
    10681 taaaatgaat tatgcatttg tggtgcaggc acgtttaggt tctacacgtt tgcctggaaa
    10741 aatattgaaa cctttctatg gtaatcaatc gattcttgac ttaatggttc ataagctttc
    10801 tgctatatcg aatattccag taattattgc tacaacaaat tctgttatca atgaacctat
    10861 tgaaaagaag gcacttgcat taggggtaaa gtgcttcaga ggtgaggaga atgatgtttt
    10921 aaaacgattc attgatgttg ctgaatattt tgatatacag ggtatattcc gaatatgttc
    10981 tgataatcca tttttagatg tacatgcggc aaggcaattg gtcgaaatag caatgaaatc
    11041 ttgtaacgat tatataagtt ttgatattga tggtactcca tcaattaaaa ctcatttcgg
    11101 tttttgggga gaatttgtaa cgcttgatgc gctgaagcga gttataggat ttactgatga
    11161 tttattgtat cgtgaacatg ttactaatta tatatatagt catcctgaac tttttaatat
    11221 tcaatggatt tcaggatctc cagtcgtgtc aaaacatcat aatattcgac tcacgattga
    11281 tacgttagaa gatttttctg tagcacagag aatatatagg gatttacaag aaaaaagagt
    11341 ggaaatatcg atagaagtga ttgtagacta tgttcgaaga catgcagaat acattcaact
    11401 gatgaaacaa caaataaaaa aaaattctaa ataatgaaga atacttatat tattggtgaa
    11461 atagggcaga atcataatgg ttctgttgac ttggctaagt tgattattga attaatctca
    11521 cgacctgtac atgaggatgt ttttggtatg gatttcatgc caatgaatgc tgttaaaatg
    11581 actaaaagag atttatcaga agaattggct gtttctcaaa tgaatcaaat atatgacaat
    11641 cctaattctt ttgggcgtac ttatggtgaa catcgtgctt ttcttgagct tgatgatcaa
    11701 gcgcattttg agatttataa atatgctaag tcattaggat tagattttat cgagacgtta
    11761 tgtgccaaag gatgtctgtc tttgcttaaa ctgttcacac cggataggct aaaagttgct
    11821 agtcgtgatt tgactaactt accattgctt gaagcattag cagaaactca tattcctatt
    11881 atattatcga caggaatggc tggtcaaaga gaacttgaca atgcattaga tgtgataacg
    11941 agatatcata gtaatatagc tattctacat tgtgtatctc agtatccaac tcatccaaat
    12001 aatttgaatc tacgtacaat aacatatttg aagaagcatt acaataagtt tgagataggt
    12061 ttttctgatc atacaatagg tatttctgct gctacagctg ctgtagcgat gggagcggaa
    12121 attatagaaa agcatgtaac aattgatcgg catatgaaag gaacagacca gcttggctct
    12181 gtaggtccag atggggttaa tcgtatgatt cgagatattc gcatagcaga gtgttggctt
    12241 ggtactgaag atctttatat agaaaaaggg gtggagaagt ctaagataaa acttgaacgt
    12301 tctattgcca ctcgaaaata tattccagca ggtagtatta ttcaagagtc ggacattcat
    12361 ttactgagtc ctggtgatgg ttataaatgg atagataaaa aaaatgtaat tggtcatttt
    12421 gctaaacaag atatatcagc gaatgaaata atatatcctg attttattga atagatatat
    12481 tcctactgca acttgtatta cggtatagag tactgcctgc tataagctaa gtagtgatta
    12541 ataaataagg gtaaaatact aaaaaaatga aagagataaa gcttatactt acagatattg
    12601 atggcgtttg gactgatggc ggaatgtttt at
//
