LOCUS       gnlProkkaBGAKDIMG_2     4733 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BGAKDIMG_2.
ACCESSION   gnlProkkaBGAKDIMG_2
VERSION     gnlProkkaBGAKDIMG_2
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-01 12:13:47
            Original ID  :: gnl|Prokka|BGAKDIMG_2
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4733
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4733
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[441:4715]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      442..4715
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..4733
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..4733
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             complement(3..425)
                     /NRPS_PKS="Domain: NAD_binding_4 (8-127). E-value: 7.6e-09.
                     Score: 26.9. Matches aSDomain:
                     nrpspksdomains_ctg2_1_NAD_binding_4.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score: 72.7;
                     E-value: 5.6e-22)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg2_1"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 3.2e-53,
                     bitscore: 169.1, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.3e-11, bitscore:
                     32.5, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDKVLMITGGTGSFGNAVLKHFINSDLKEIRIFSRDEKKQEDM
                     RIEYKNDKLNFIIGNVRDFDSINNAMAGVDYVFHAAALKQVPSCEFYPMQAIRTNLYGA
                     ENVLEAAARNNVKKVVVLSTDKAAYPINAMGMTKALM"
     aSDomain        complement(45..401)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg2_1_NAD_binding_4.1"
                     /evalue="7.60E-09"
                     /label="ctg2_1_NAD_binding_4.1"
                     /locus_tag="ctg2_1"
                     /protein_end="127"
                     /protein_start="8"
                     /score="26.9"
                     /tool="antismash"
                     /translation="ITGGTGSFGNAVLKHFINSDLKEIRIFSRDEKKQEDMRIEYKNDK
                     LNFIIGNVRDFDSINNAMAGVDYVFHAAALKQVPSCEFYPMQAIRTNLYGAENVLEAAA
                     RNNVKKVVVLSTDKA"
     CDS_motif       complement(315..401)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg2_1_0001"
                     /evalue="6.40E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="ctg2_1"
                     /protein_end="37"
                     /protein_start="8"
                     /score="12.4"
                     /tool="antismash"
                     /translation="ITGGTGSFGNAVLKHFINSDLKEIRIFSR"
     CDS             complement(442..1551)
                     /NRPS_PKS="Domain: Aminotran_5 (30-298). E-value: 6.3e-34.
                     Score: 109.3. Matches aSDomain:
                     nrpspksdomains_ctg2_2_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 80.5; E-value:
                     1.9e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg2_2"
                     /sec_met_domain="Aminotran_5 (E-value: 3.9e-32, bitscore:
                     99.9, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIERKILLNPGPATTTDTVKLAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGDLSKHTAVLFCGSGTINIDICLNSLLPADKKVLVVNNGAYSTRAVEVCEYY
                     GLPHIDLKFSVYELPDLSVIEKTLSDNPDIALVYTTHHETGTGILNPIREIGKIAHSHG
                     AVFVVDTTSSLGMIPFDIGKDNVDFCMASAQKGLQAMTGLSFIIGDESLIRASRDYPKR
                     SYYCNLYLQYDYFEKTGEMHFTPPVQTVYAARQALDEYFAVGEEAKFARHKRVFEAIHK
                     GLQELGLQYAIKREWQSGLVVSVLYPDDPLWDFDKVHDYCYERGFTIYPGKISTTNTFR
                     LCALGEIDVEDIERFFEVMREAKMSLNNK"
     aSDomain        complement(658..1461)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg2_2_Aminotran_5.1"
                     /evalue="6.30E-34"
                     /label="ctg2_2_Aminotran_5.1"
                     /locus_tag="ctg2_2"
                     /protein_end="298"
                     /protein_start="30"
                     /score="109.3"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGDLSKHTAVLFCGSGTINIDICL
                     NSLLPADKKVLVVNNGAYSTRAVEVCEYYGLPHIDLKFSVYELPDLSVIEKTLSDNPDI
                     ALVYTTHHETGTGILNPIREIGKIAHSHGAVFVVDTTSSLGMIPFDIGKDNVDFCMASA
                     QKGLQAMTGLSFIIGDESLIRASRDYPKRSYYCNLYLQYDYFEKTGEMHFTPPVQTVYA
                     ARQALDEYFAVGEEAKFARHKRVFEAIHKGLQELGLQYAIKREWQS"
     CDS             complement(1548..2684)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 70.6;
                     E-value: 1.9e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg2_3"
                     /sec_met_domain="TPP_enzyme_N (E-value: 7.5e-23, bitscore:
                     69.4, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.5e-21, bitscore:
                     64.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVSVEKIYDTFLHHGVDFFTGVPDSLLKNICAYITDHAPRGKHII
                     AANEGSAVGIACGYYMASGNVPLVYMQNSGLGNTVNPLLSLADEKVYSLPLLLLVGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMKIPFIILDTDEDEALAQIHDAVVSAKSRSIPHAIV
                     IRKDTFGKYKLRQEALNDYPLSREEALMQVVDHLGDNDIVVSTTGKLSRELFEYRESKG
                     QGHARDFLTVGSMGHSSSIALGIALEKPDRRVYCLDGDGAFIMHMGAVSNIGDLSPKNY
                     RHVLFNNGAHESVGGQPTLGFRLDIASIAKGCGYAHVLTASDKEGLSCALEKLSGLSGP
                     VLLEIKVRIDSRDDLGRPTTTPVENKEHFMAFLKDTHS"
     CDS             complement(2696..3997)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 214.1;
                     E-value: 3.9e-65)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg2_4"
                     /sec_met_domain="CTP_transf_like (E-value: 1.3e-13,
                     bitscore: 39.9, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 4.6e-123, bitscore:
                     398.2, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 2.3e-65, bitscore:
                     208.8, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKKIYVGMTADIMHPGLIHIIHEATKYGDVIVGLLTDKAIAEHK
                     RLPYLTYDQRKEVVENIKGVCEVVPQEDWSYVPNLEKLKPDYIIHGDDWKTGPLREVRE
                     QVFEVMNAQGGKVIEIPYTRGINSSSLDKDIKAIGTTPDVRLKSLRRLIQAKPVVRILE
                     AHDGLCGLIIENLEVQKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAVIIEDKVGLKKNSLFGTDAIQTQDS
                     IEGFCDKIRAGKNAQVTGDFMIIARIESLIAGKPMSDALERAYAYVQAGADGIMIHSKD
                     KSGEDIREFCRTFRKEYAHVPIVVVPTTYDHVHESELHEWGANVVIYANHLLRAAYPAM
                     MNVARTILENERAEEVRPLCMPIKEILELIPGTK"
     CDS             complement(4017..4715)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg2_5"
                     /sec_met_domain="NTP_transf_3 (E-value: 3.9e-15, bitscore:
                     45.0, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAGLGTRFGHYTELVPKGFVEVGGKPMIIRSIETLLSCGIERII
                     LGTGYKKEAYEALQADFPQIETCFSPRYADTNSMYTLYNTRDVIGDDNFLLLESDLVFE
                     RKAILSLLDDEFPDVMLVSSLTKFQDQYYVEYDCNHILTSCSVDKDALEAKGELVGIHK
                     LSNTFYRRMCADYATILESQPKLGYEYELLRMSRSVSPVRVLRVEGLKWYEIDDEADLS
                     YAEEHIIRYC"
ORIGIN
        1 tccattaagg cttttgtcat tcccatagca tttataggat aagcagcctt atcggtactc
       61 aaaacaacaa cttttttaac attattacga gcagcagcct ccaatacatt ctcagcgcca
      121 tacaagttag tacgaatagc ctgcattgga taaaattcac aagaaggtac ttgttttaat
      181 gcagcagcat ggaaaacata atcaacgcct gccattgcat tgttaatact atcaaaatca
      241 cgcacattac cgataatgaa atttaattta tcgtttttat attcaatgcg catgtcttcc
      301 tgcttctttt catcacgact gaatattctg atttctttta aatcagaatt aataaaatgt
      361 tttaaaacag cattaccaaa agagcctgtt cccccagtaa tcattagaac tttatcttta
      421 aacatatatc atactattat cctatttatt attcaaactc attttcgcct ccctcatcac
      481 ctcaaagaac ctctcaatat cctccacatc aatctctccc aacgcacaaa gccggaatgt
      541 attcgttgtg gaaatcttac ccgggtagat cgtaaagcca cgttcatagc agtaatcatg
      601 aaccttatca aaatcccaga ggggatcatc aggatacaat acagaaacaa caagacccga
      661 ctgccattca cgtttgatcg catactgaag accaagctct tgcaaacctt tatggatggc
      721 ttcaaagacc ctcttgtgac gggcgaattt agcctcttca cctactgcaa aatattcatc
      781 aagagcctga cgggcagcgt aaacggtttg aacaggagga gtaaaatgca tctcacccgt
      841 cttctcaaag taatcgtatt gtaaatagag gttacaatag taggaacgtt tcggataatc
      901 ccttgaagca cgtatcaggg attcatcacc tatgataaag gaaagacccg tcatggcctg
      961 aagacctttt tgagccgaag ccatacaaaa gtcaacatta tccttcccga tatcaaaagg
     1021 aatcataccc agggaggaag tggtgtcgac aacaaaaacg gcaccgtgcg aatgggctat
     1081 ttttccgatc tcacggatgg ggttcaagat acccgtaccc gtttcatggt gcgtggtata
     1141 gaccaaagcg atgtccggat tgtcggaaag cgtcttttcg attaccgaca ggtcgggaag
     1201 ttcgtaaacg gaaaacttca aatcgatatg gggaagaccg taatattcgc agacttccac
     1261 cgcacgggtg ctgtaggcac cgttattgac aaccagaact ttcttatccg caggaagaag
     1321 agagttcagg cagatatcga tattgatcgt acccgagccg caaaacagga cggccgtatg
     1381 tttggagagg tctccatgcg caacgcggac caggtcgtca cgtaactgtt tcatcatacc
     1441 cgcaaactct ttctcacgcg gacagatatc gggaacaacc tgcgccagct ttaccgtatc
     1501 cgtcgtggtg gcgggacccg gattcaaaag gatctttctt tcgatattca tgaatgcgta
     1561 tcttttaaaa atgccataaa atgctcttta ttctctaccg gagtagtcgt agggcgaccc
     1621 agatcatcac gtgaatcgat ccggacctta atttcaagaa gaaccgggcc ggacaggccg
     1681 gacagcttct caagcgcaca cgaaagccct tccttgtcgg aggcagtcag tacatgcgca
     1741 taaccgcaac ctttcgctat cgaggcgata tccagacgga aaccgagcgt gggttgaccg
     1801 ccgacagatt catgggcacc attgttgaaa aggacgtgcc tgtaattctt gggagaaaga
     1861 tcgccgatat tgctgacagc acccatgtgc atgataaagg caccgtcacc gtcaagacag
     1921 tagacacggc ggtcaggctt ctcaagggca atccccaggg caatggaaga actgtgaccc
     1981 atggaaccca cagttaggaa atcccttgcg tgcccctgcc ctttcgattc acggtactcg
     2041 aaaagctcac gggagagctt gcccgtggtg gaaaccacaa tgtcattgtc cccgaggtgg
     2101 tcgacaacct gcattagcgc ctcctcgcgg gacagaggat agtcattcaa ggcttcctga
     2161 cgcaatttat attttccaaa cgtatcttta cggatgacaa tcgcatgggg aatcgaccgg
     2221 ctcttcgccg agacaaccgc gtcatggatc tgcgccaggg cctcgtcttc atccgtatcc
     2281 agaattataa aaggaatttt catggctttc agaagatcaa gggtaacctc gccctgcttc
     2341 ttatgctggg gttcgtcttt cgtgccgggc tcaccgcgcc agccgaccag caaaagaaga
     2401 ggaaggctgt acactttctc gtccgcaagg gacaagaggg gattcaccgt atttcccaga
     2461 ccggagttct gcatataaac caaaggaacg ttgccagaag ccatataata accgcaggca
     2521 atgcccaccg cggaaccttc attggcggcg atgatatgct taccacgcgg agcatgatcc
     2581 gtgatatagg cgcaaatatt cttcaaaagg gaatcgggaa cacccgtaaa gaagtcaaca
     2641 ccgtgatgaa ggaacgtatc gtatatcttt tctacactta ccataaccgt ctgatttatt
     2701 tagtgcccgg aatcaattcc agaatttcct tgataggcat acaaagcgga cggacctcct
     2761 cggcgcgctc gttttcaagg atggtcctgg ccacgttcat catggccggg taagcggcac
     2821 ggagaaggtg gttggcgtaa atgactacgt tggcacccca ttcgtgaagt tcggattcgt
     2881 gcacatggtc ataagtcgtc ggaacaacga caatgggaac atgggcgtat tctttgcgga
     2941 aagtccggca gaactcacgg atatcctcac ccgacttatc cttgctgtgg atcatgatgc
     3001 cgtccgcacc cgcctgtaca taggcataag cacgttcgag ggcgtcactc atgggtttgc
     3061 cggcaataag gctttcgata cgcgcaatga tcataaagtc accggtgacc tgggcattct
     3121 tccctgctct gatcttatcg cagaaaccct cgatactatc ctgagtctgg atagcgtcag
     3181 taccaaaaag agaattcttc ttcagaccga ctttatcttc aatgattacc gcagaaatgc
     3241 cgtgacgttc cagagtgcgc acagtaaaga caaagtgttc gatcttgcca cccgtgtctc
     3301 catcaaaaat aataggcttg gtggtacatt caaggatatt gttcaggtcc tgtaaacggg
     3361 tggtaaggtc gaccgcttca atatccggtt tacccttact cgtagagtcg gtcaggctgc
     3421 tggaccacat gccgtcaaag acctcgcgct tatcaccctt ctggacttca agattctcaa
     3481 taatcagacc gcaaagaccg tcgtgagcct ccaggatacg gaccacaggc ttggcctgga
     3541 tcaggcggcg gagggactta agacgtacgt caggggtagt cccgatcgcc ttgatatcct
     3601 tgtcgagcga ggaggagttg atgccccggg tataagggat ctcgattacc ttgcccccct
     3661 gggcgttcat cacctcaaaa acctgttcgc gcacctcgcg caacgggccc gttttccagt
     3721 cgtcaccgtg gatgatatag tcaggcttga gtttttccag attcggaaca tagctccaat
     3781 cctcctgggg aacaacttcg caaacccctt tgatattctc aacaacctcc ttacgctgat
     3841 cataggtaag gtaagggagg cgtttgtgtt cggcgatggc cttgtccgtc agcagaccga
     3901 cgatgacatc accgtattta gtagcttcat gaataatatg gatcagcccc ggatgcatga
     3961 tatccgcggt cataccgaca tagattttct tgttcatata tggaaaagat ttgtttttaa
     4021 caatagcgga tgatgtgctc ctcagcatac gaaaggtcgg cttcatcatc gatttcatac
     4081 catttaagcc cctccaccct gagcacacgg acaggggaaa cggaacggga catgcggagc
     4141 agttcgtatt cgtaacccag tttaggctgg gactccagga tggtcgcata atcggcgcac
     4201 atccggcggt agaaagtatt ggaaagtttg tggatgccga ccagctcgcc ttttgcctca
     4261 agggcgtcct tgtctaccga acaggaagta aggatgtgat tgcagtcata ttccacataa
     4321 tactgatcct ggaacttggt cagagaagag acaagcatca catccgggaa ttcgtcatcc
     4381 agtagcgaga ggatggcctt gcgttcaaaa acaaggtccg attcaagcag caggaaattg
     4441 tcatcgccta tcacgtcacg cgtattgtac agagtgtaca tactgttagt atcggcataa
     4501 cggggactga aacaggtctc tatctggggg aaatccgcct gaagggcttc ataagcctct
     4561 tttttataac ccgtaccgag gatgatacgt tcgataccgc aggaaagaag ggtttcgata
     4621 gagcggataa tcataggttt tccaccaacc tccacaaaac ctttgggcac aagctcggta
     4681 taatgcccga agcgggtacc aagaccggca gccatgatca ttgctgtttt aat
//
