LOCUS       c00149_gnlPro..         7240 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BJCOKJNE_149.
ACCESSION   c00149_gnlPro..
VERSION     c00149_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 21:58:46
            Original ID  :: gnl|Prokka|BJCOKJNE_149
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7240
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7240
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[4182:7153]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4183..7153
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7240
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7240
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             24..329
                     /locus_tag="ctg149_1"
                     /transl_table=11
                     /translation="MEAKTNEEFFNVSALIDERFGKEGTASRAEAEEKAYAFYTGQIIE
                     DARKKAKITQAELARRIGSDRSYISRVESGQTEPKVSTFYRIMNALGCKIEFSMIL"
     CDS             complement(399..2513)
                     /locus_tag="ctg149_2"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GEKAANEKKKELPAIAFSASYRGRRTKVNLVKYLGHIVIDIDHLSKEELARILPIIKRC
                     DYTRIAFISPKGMGVKIIVRACHPDETLPETLQEIEDFHHAAYTRLVSFYTELCQIEID
                     TSGQDVARTCLFSYDPEIYFNPNADAFLVDQPQASYKVSNRKNLSGSKQQTPPDGPPTN
                     EDTALNAHSANASLVLTLTYYHNKSEKYIVGNRNNYLHHLSCTFNRYGIPQEEASAFIK
                     SQFTDFPADETASLINSAYAHTDEFNTCKLNGTQKRILRIEQYISEHYETRYNEVLHIM
                     EYRRRHPDTEKPEPFRILDEMMENSIWIEINELGYACTVKTIENLIYSDFSQSYHPIRE
                     YFELLPEWDGTDYIRILADSVQTSHQEFWAECLERYLVAMCAAATQENIVNHTVLLLCS
                     EIQNIGKTTFINNLLPPELRAYLSTGLINSNNKDDLAKITQAMLINLDEFEGMSGRELN
                     AFKDLVTRKVISFRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHPYSIQF
                     ITINYAQLYAQIKYLLNKPGYQYWFTQADNERLEENNEAFIFHSPEEEMVLTHIRKPER
                     FEKVYYLTVSEIAELIRERTGYQYSIGSKIQLGKVMTKHHFESKKGNNGRRYAVFIIDI
                     EQVKSNRLYE"
     CDS             complement(2600..2830)
                     /locus_tag="ctg149_3"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             3024..3527
                     /locus_tag="ctg149_4"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLIFRPGKTLAELMKILSYQQVQTRAVAQAGAGDGGGEDPDKNPDSGGDGSGDEEAP
                     DPTV"
     CDS             3587..3754
                     /locus_tag="ctg149_5"
                     /transl_table=11
                     /translation="MKEIVSRILDVIMYLIPFFGKRKRDRVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             3741..4190
                     /locus_tag="ctg149_6"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(4183..4911)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 71.7;
                     E-value: 2.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg149_7"
                     /sec_met_domain="Radical_SAM (E-value: 1.8e-29, bitscore:
                     92.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKTLVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(4925..7153)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg149_8"
                     /sec_met_domain="Gly_radical (E-value: 5e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     KFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 aaagatatta agaaaggagt attatggaag caaaaacaaa tgaggaattc tttaatgtca
       61 gtgcattaat agatgagcgt tttggtaagg aaggaacagc ttctcgtgca gaggcagaag
      121 aaaaggccta cgccttttat acaggacaga ttatcgagga cgcaagaaaa aaggctaaaa
      181 tcactcaggc tgaattagcc cggcgtatcg gctctgaccg ttcttatatt tcaagagtag
      241 aaagtggtca gacagagcct aaagtatcta ctttctatcg tataatgaat gcgttaggct
      301 gtaaaatcga attttcaatg attttgtaaa aacaataaat gcagagtatc tgtcaagatg
      361 actctgcatt ttgctatatt cacaaggctt tcccgcttct attcatacag cctgtttgat
      421 tttacctgct cgatatctat aataaatacg gcatatcggc gcccgttatt tcctttttta
      481 gattcaaaat ggtgcttagt catcaccttc cccaactgta tttttgatcc gatggaatac
      541 tgataaccgg tacgctcacg tattagttcc gcaatctcac tgacagtcag ataataaacc
      601 ttctcgaaac gttccggctt gcggatatgc gtaagaacca tttcttcttc cggtgaatga
      661 aagatgaaag cctcgttatt ctcttcaagc cgttcattgt ccgcttgcgt gaaccagtat
      721 tgatatcccg gcttattcag cagatatttg atctgagcat agagctgcgc ataattgatg
      781 gtaataaatt ggatggaata aggatgaaaa caaaggaaac gccggttgcc tgtcgtgtcg
      841 tgaagcactt cctgataatt gcaggttcct gcaaaagagg ccgtatgagg gaagttttgc
      901 gaacggcgtg cataaggcag acggaaactg atcactttgc gcgttaccaa atctttgaat
      961 gcattcagtt cccgaccgct catcccttca aactcgtcga gattgatcag catggcctgc
     1021 gttattttgg ctaaatcatc tttattgttc gaattgatta gtccggtaga gagataagca
     1081 cgcagttcgg gaggcaggag attattgata aaggtagtct ttcctatgtt ctgtatctcg
     1141 ctgcatagca gaagtaccgt gtgattgaca atattctctt gagtagccgc tgcgcacatc
     1201 gccaccagat aacgttccag gcattccgcc caaaactcct gatgactcgt ctggacacta
     1261 tccgccagga tacggatata gtcggttccg tcccattcgg gcagtaattc aaaatactcg
     1321 cgaatgggat gatacgactg gctgaaatcg gagtaaatca gattttcgat tgttttgaca
     1381 gtgcaggcat atcccagttc gttgatttct atccagatac tgttttccat catctcatcc
     1441 aagatgcgga aaggctccgg tttctcggtg tcgggatgcc ttctgcgata ttccatgata
     1501 tgcagcactt cgttgtagcg tgtttcgtag tgctcgctga tatattgctc tatccgtaat
     1561 attcgtttct gcgtgccatt tagtttacag gtattaaatt cgtcggtatg agcgtaggcg
     1621 ctattgatca aagaggctgt ctcgtccgcc ggaaagtctg taaactggct tttgataaag
     1681 gcggatgctt cctcctgcgg aatgccgtag cgattgaagg tacatgagag atggtgcaga
     1741 taattgttcc ggttgcccac tatgtatttt tcggacttgt tgtgatagta ggttaaagtc
     1801 agcaccagag aagcgttggc ggaatgggcg ttgagcgctg tgtcttcatt cgtcgggggg
     1861 ccgtccggag gagtctgttg tttactgccg gagagatttt tacgatttga aaccttgtag
     1921 gatgcctgag gttgatctac aagaaaagcg tcagcgttcg gattgaaata aatctccgga
     1981 tcgtaagaga acagacaagt gcgtgccaca tcctgaccgg aggtgtcgat ttctatctga
     2041 cagagttcgg tgtaaaaaga aacgagtctg gtgtaagctg catgatggaa gtcttctatt
     2101 tcctgaaggg tttcgggcaa tgtctcatcc ggatggcatg cgcgtactat gattttcacg
     2161 cccatccctt tggggctgat aaaggcgatg cgggtgtaat cgcatctctt gatgataggg
     2221 aggatgcgtg ccaattcttc tttgctgagg tggtcgatgt ctatcacgat gtgtcccaga
     2281 tatttgacga ggttcacttt ggtacgcctt cctcggtagc tggcggagaa ggcgattgcc
     2341 ggcaattctt ttttcttctc attggcagcc ttttctcctt gctctgcaaa gatgcggcgt
     2401 atttcttcga ctttttcccg gtaggtaaaa ctgccgatag tggacactat ttcttcttcg
     2461 gtcatctctg ctattgcgta gtagaaacct tgaaatatgg tgaaatgttt catttttatt
     2521 ttgattattg tttgtcgatg caaaggaact tctttgcgga aatgtgtaaa ggaactttgt
     2581 ataaaatggc aataagttac tacggttcgc ccaaaaagtg tatgatagtt gccacttgtt
     2641 tcggcgaaag gagacggttg cgtgcgcggt agccttcctg ttcgagttcg tgcaggagag
     2701 ggaggttgta aataatccaa cgacgaagta tttgtaaagc aactttgagg cagacatggg
     2761 gattgtaaag gtgagccagt tccgctttgg tataggtgcg gataaggaaa gaatcttctt
     2821 tgtaattcat gatgttttct tcttgtttta tgctataaag ttacataaaa ttccttgtta
     2881 ttcaaaggtt tgacttcgtt tttattcgtt gggaatcaag aaacttcgtt ggggaatatg
     2941 gcttgtccgg ctatccttat ctttgtgctg ttaacaagaa ggggatgcat cctatgtatt
     3001 ataaacttta aaattatttt attatggcaa tgacagtatc ttattcagtg gtgccgcgca
     3061 agaatcctgc aaaaaagagc gaaccggcaa agtattatgc acaggcgcag gcgtcgggag
     3121 agttggactt tgaagagttg tgcgaagcga tcaccagtcg ttctacttgt acggaaacgg
     3181 atgtacgtgc ggctatttcg ggtattcttt acgaagtgaa gcgtgcgttg aaggcaggaa
     3241 gaattgcgag actgggcgat ttgggcagtt tgcagatagg actcaacagt gaaggtgcgg
     3301 catcggtcaa ggaattttcc ggttcaatga ttaagggggc gcatcttatt ttccgccccg
     3361 gtaagacgct ggcggagttg atgaagattc tgagttatca gcaagtgcag actcgtgcgg
     3421 tggcgcaggc gggtgcaggc gatggcgggg gagaagaccc tgacaagaat ccggattccg
     3481 gtggtgatgg ctccggtgat gaagaggctc cggaccctac ggtataattg gcagacgggt
     3541 caaggtaata acgaattaga ataacttcca ttaatatttt gataagatga aagaaatagt
     3601 aagtcggatt ttagatgtga tcatgtatct gattcctttc ttcggaaaga gaaagcggga
     3661 cagggtggtg agagaagtgc gctatcatac tacttgtaag gaggtgtgca aagtgaaaac
     3721 gacggaaagg gaaaaggaac atgagaaaga ttagtctgat tgtgatccat tgttccgcca
     3781 cccgtgtcga ttgcgacttt acggcaaagg atgtggatac ggctcaccgt tatcgtggct
     3841 tttcgtgctg ggggtatcat tattatatac gcaagtcggg cgagattgag ccgatgcggg
     3901 atgaagatac ggtcggagct catgcacgtg gctataatgc gatcagctta ggtgtatgtt
     3961 atgagggcgg tctggatgag aatggaaaag ctgcggatac gcgtacaccc cgtcagaagg
     4021 aggcgctaca tcgcttggta cacgaattgt tgcagcgtta tccggaagct aaagtagtag
     4081 ggcatcggga tttaagtccc gacaccaact ataatgggat cgtagatcct tgggaacgga
     4141 ttaaagaatg cccttgcttt gaggtcattg gagaattctc cattaattaa ctaccaccgt
     4201 agtgaaatac tctttgaata cctccgcagc tttttcaagc tgttcggggg tattttcttt
     4261 tacccctttc atcttgtatt cctgtcccat cgcttcgtac ttatgtacgc ccagtgtatg
     4321 gtaaggcagt atttccaccc gttggatcat cttgtatttt ccgagtgctt ctcccagtgc
     4381 gcgtatgtct tcttcaaaat cgctgtatcc gggcactagc acgtagcgta gccagaaagg
     4441 tttctcctgt tcttccagcc aggcggccgt gcggatggtt tgctcgttgc ttcttccggt
     4501 gagcgtctga tggcggttgg ggttgaattc ctttatatcc aataacacaa gatcggtcag
     4561 tctgaataat tcttctacgt cttcgttcca aagtcctccg ttgctgtcca gacagacatg
     4621 gatgcctttt tctttcagtt cgcggaccag cgggacgagt gttttcgcct gaaacgtcgg
     4681 ttcgcctccg gagaaagtga ttcctcctcg tttcccgaag aaggggcgct ggctcattgc
     4741 catgcggacg atttcttccg gtggggtagg cgtgcctccc tttcctgcta tcgtatccgg
     4801 attggcgcaa tagaggcagc ggaagttgca tccttgaaga aaaacgacga gccgtaagcc
     4861 cggcccgtcg aatgttccca tactttcgta tgaatgtacg tttatcatca tatagagtaa
     4921 tagattacat acgttcgtgg aagctacggc tgataacttc cagttggtgt tcgcggctca
     4981 gcttcacgaa gtttacggca tagccggaaa cgcggatagt cagctgcgga tagttttccg
     5041 gatgctccat ggcatcgtac agcatatcac ggttcagtac gtttacgttc aggtggtgag
     5101 cacctttggt gaagtaaccg tccatcatcg ttacgaggtt ctcgatacgg tcttcgtccg
     5161 tagcacccag tgatttcgga acgatagaga aggtgttgct gataccgtcc tgtgagtcac
     5221 ggtaacgaag tttcgctaca gagctcagag aagcgatcgc accattcttg tcgcgtccgt
     5281 gcatcgggtt ggcacccgga gcgaaggcaa cacctttggc acgtccgtcg ggagtagcac
     5341 cggtcttttt gccgtacatc acattggaag tgatagtcag caaggagagg gtaggacggg
     5401 cattcttgta aactggcaat ttcttcaatt cttcgctgaa gaagtatacc aggtctacgc
     5461 caaggtggtc taccttgtcg ttgtcattac cgaagcaagg gaattctcct tcgatgtcga
     5521 aaccttctgt caggccgata tcgttgcggc gggcagttac tttcgcatat ttgatggccg
     5581 acagtgaatc gagggcgata gaaagtccgg ctacaccgta agccaggttg atgcgcgggt
     5641 tcgtatctac aagagccatc tgggccttct cgtagtaata cttgtcgtgc atatagtgga
     5701 tgatgttcat cgcttcgtta tatacgcggg cgatttcggt cagcactttt ttgtagttgt
     5761 ccattacttc ttcgaacttc agcgtgtcac tggtcagtac ggggatgttc ttcaccatga
     5821 ctgtaccggt attctcgcaa cgtccgccgt tgatggcaag caacagggct ttggccaggt
     5881 tgcaacgggc accgaagaac tgaatctgct tgcctatttc ctggtatgat acgcagcagg
     5941 cgattccgta gtcgtcggac tgacgtactt cacgcatcag gtcgtcgttt tcgtactgga
     6001 tagaagaagt gtcgatagaa acctttgcac agaattcttt gaatccttcc ggaagttccg
     6061 ggctccagag tacggtcagg ttcggttccg gtgaagggcc gaggttgtac aatgtctgca
     6121 gaaaacggaa ggaggtcttt gtcactttgg tacgtccgtc gttgagacgt ccgcccagag
     6181 attcggttac ccaggtcggg tcgcctgcaa agatgtcgtt gtaagattgc atacgcaggt
     6241 ggcgcaccat acgcagtttg atgacaaact ggtcgatcag ctcttgcgcg aacgattcgg
     6301 tgatggttcc tttgctcagt tcatattcca gatagatatc gaggaaagaa gaaacgttac
     6361 ccagtgacat agcggcaccg tcttgttctt tgacggcggc aaggtaagcc atatataccc
     6421 actgtacggc ttcttgtgcc gtgtaagcgg gacggctcag gtcgagaccg tagtattcgc
     6481 ccatcacttt catgtctttc agtgccttga tctgttctgc cacttcttcg cgcaggcgga
     6541 tgcgggcctc tgtcatcgga ccggtgaggt tgtgcaaatc ttccttcttt gcttcgatca
     6601 gacggtcgat gccgtaaaga gccatacggc ggtagtcacc gatgatgcgt ccgcgtgcat
     6661 aattgtcggg aagtccggta aggaatccca gcgaacggaa ggaacggatt tcttccgtat
     6721 atacgtcgaa tactccgtcg ttgtgcgtct tgcggtagtg agtgaaaata tcttttacgc
     6781 ggtcgtccac ttccacgccg ttttcgtgac aggctttgct gactacgttg atacctccga
     6841 aaggcttgat ggcacgtttc agaagttcgt ctgtctgcag gccgacgatc agttcgtttt
     6901 ccttgtcgat atatccggcc ttgtgggaag tgatggttga tacggtaaca ttatctaatg
     6961 agcggacacc gttgttttct ctttcttctg ccagtgcttc gaggcagcgg ttccatacgg
     7021 ctttggtacg ttctgtaggt ccttcgagga atgaagcatc tccgtaatac ggagtgatgt
     7081 tatgacttac gaaatctctg acgttgattt cgctgctcca aagaccgtct ttaaagatct
     7141 tgtttaattc cataaaatga taaaattaga ggttgtagtt atcctattct ttagagcgca
     7201 caaagttaca acgttttttc atataatcag cataaatatg
//
