LOCUS       c00043_gnlPro..         6956 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LMFPGLLE_43.
ACCESSION   c00043_gnlPro..
VERSION     c00043_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 16:57:12
            Original ID  :: gnl|Prokka|LMFPGLLE_43
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6956
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6956
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2413:6954]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2414..6954
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..6956
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6956
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             3..785
                     /locus_tag="ctg43_1"
                     /transl_table=11
                     /translation="AVEYAKELGCKNPEIVLHLDHGDTFETCKSCIDSGFSSVMIDGSH
                     LPYDENVALTKKVVEYAHQFDVTVEGELGVLAGVEDEVSSDHHTYTEPDEVVDFVTKTG
                     CDSLAISIGTSHGAYKFTPEQCHIDPKTGRMVPPPLAFDVLDGVMKELPGFPIVLHGSS
                     SVPEEEVATINQFGGALKAAIGIPEEELRKAAKSAVCKINIDSDSRLAMTAAIRKTFAE
                     KPAEFDPRKYLGPARDNMEKLYKHKILNVLGSDNKLAE"
     CDS             complement(865..2199)
                     /locus_tag="ctg43_2"
                     /transl_table=11
                     /translation="MKRIILLAFLCTTTLVMAQRINHVQEAIANYDYEAALTLIAKEKP
                     TIPLLLQKGKAQRGLGMNTEALSTYQEIITNDTTNTRAYIEAAECCRSLAKYQQALKYY
                     EQALDLNPENKYVRIQYIGLLLSLQKFQDALGESSLMTEKDSSAIALHLQAQSFEGMGE
                     LLPATGCYYNIQEKYPDDYLAAAKLAALNIAGSYFNEAIEATEKYRQIDTTNIAVNRQN
                     ALAYCLNKDYPTAIQRYEYLVSQGDSSFHTCYYLGISYYAEEKYYEAHDFLEAARKHDP
                     ENVNLLYYLGRACAKTSWKKLGVEYLEKALDLTIPKDSNMVRLYIGMTDCYKMAQMPKE
                     QIESIRERYRKYDKQNHKLLYDMAFIYFYSLKDKKNTERCLEAFLKTRPKEDKEEEAKL
                     NERGELVLGTKNYYNAAANWLKDIQSKQKIEDFFLGGNVPAQKPE"
     CDS             complement(2414..3574)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg43_3"
                     /sec_met_domain="OAD_beta (E-value: 1.8e-154, bitscore:
                     502.7, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDFINFLGNNLADFWTYTGFANATVGHVVMLLVGLFFIYLAVAK
                     EFEPMLLIPIGFGILIGNIPFNMDAGLKVGIYEEGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYMIALEMGFDPMQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPVIQPPIMRLLTTKHERVIRMKPPRAVSHTEKVIFP
                     IIGLLLTCFLVPSGLPLLGMLFFGNLLKESGVTRRLANTASGPLIDTITILLGLTVGAS
                     TQASEFLTLDSIKIFALGALSFIIATASGVIFVKIFNLFLKKGNKINPLIGNAGVSAVP
                     DSARISQVVGLEYDPTNYLLMHAMGPNVAGVIGSAVAAGILLGFLM"
     CDS             complement(3576..4010)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 66.7; E-value:
                     4e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg43_4"
                     /sec_met_domain="Biotin_lipoyl (E-value: 4.6e-22, bitscore:
                     66.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEYKYKINGNSYKVTIGDIEDNIAHVEVNGTHYKVEMEKQPKTA
                     PKPVVVRPMPNSPAAPTTPVVKPAAPSTGKSGVKSPLPGVILDIKVNVGDTVKRGQTII
                     ILEAMKMENNINADKDGKVTAINVNKGDSVLEGNDLVIIE"
     CDS             complement(4035..4955)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg43_5"
                     /sec_met_domain="OAD_gamma (E-value: 1.9e-15, bitscore:
                     46.2, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTKIGIFLSLLLLIGLTSCGEQKSNNKLLLNEVLIDNQNNFQD
                     DYGLHSAWIEIFNKSYGSADLAACLLKVSSQPGDTVTYFIPKGDILTLVKPRQHALFWA
                     DGEPNRGTFHTSFKLNPETANWVGLFDSGRKLLDQVVIPAGTLGPNQSYARISDGAADW
                     EVKGGSKDKYVTPSTNNKTLDSNAKMEKFEEHDSVGIGMSISAMSVVFCGLILLFIAFK
                     VVGKVAVNLSKRNAMKAKGIDKVEAKELSQAPGEVYAAISMALHEMQDEVHDVEETVLT
                     ITRVKRSYSPWSSKIYTLRENPNRK"
     CDS             complement(4987..6540)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg43_6"
                     /sec_met_domain="CT_Btheta (E-value: 2.3e-291, bitscore:
                     955.2, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKIKELIERRAVARIGGGEKAIAKQHEKGKYTARERIAML
                     LDEGSFEEMDMFVEHRCTNFGMEKKHYPGDGVVTGCGTIEGRLVYLFAQDFTVTAGSLS
                     ETMSLKICKIMDQAMKMGAPCIGINDSGGARIQEGINALAGYAEIFQRNILASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMMEGTSYMFLTGPKVVKTVTGEDVSQENLGGASVHST
                     KSGVTHFTAKTEEEGLALIRTLLSYIPQNNLEEAPYVDCTDPIDRLEDSLNDIIPDSPN
                     KPYDMYEVISAIVDNGEFLEIQKDYAKNIIIGFARFNGQSVGIVANQPKFLAGVLDSNA
                     SRKGARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTI
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVEVLYAREAKDQENPAQFLA
                     EKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRVIRALQQLQTKKLSNPAKKHGNIPL
                     "
     CDS             complement(6571..6954)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg43_7"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.2e-24, bitscore:
                     75.8, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="HLGIAVKSIEEALPYYENVLGLKCYNIETVEDQKVRTAFLKVGET
                     KIELLEPTCPESTIAKFIENKGAGVHHVAFAVEDGVANALAEAESKEIRLIDKAPRKGA
                     EGLNIAFLHPKSTLGVLTELCEH"
ORIGIN
        1 gtgctgtaga atatgcaaaa gaactcggct gcaaaaatcc tgaaatcgtt ttgcacctcg
       61 accacggaga tactttcgaa acttgcaaaa gctgtatcga ctcaggcttc tcttctgtaa
      121 tgatcgacgg ttctcacctt ccttacgacg aaaacgttgc tctgactaag aaagttgttg
      181 aatacgctca ccagttcgat gtaactgtag aaggtgaact tggcgtattg gcaggtgtag
      241 aagacgaagt ttcttctgac caccacacat atactgaacc ggacgaagta gttgatttcg
      301 ttaccaagac aggttgcgat tcattggcta tctctatcgg tacttctcac ggtgcttaca
      361 aatttactcc ggaacagtgc cacatcgatc cgaagacagg ccgtatggtt cctcctccgt
      421 tggcattcga cgtattggac ggtgtaatga aagaacttcc gggattccct atcgttcttc
      481 acggatcatc ttcagttcct gaagaagagg ttgcaactat caaccagttt ggtggtgcac
      541 tgaaagctgc tatcggtatt cctgaagagg aactgcgtaa agccgctaag tctgctgttt
      601 gcaaaatcaa catcgactca gactctcgtc tggctatgac tgctgctatc cgtaagactt
      661 tcgctgaaaa accagctgaa ttcgacccac gtaagtatct tggtccggct cgtgacaaca
      721 tggaaaaact gtacaagcac aaaatcctta atgtgctggg ttcagacaac aaattggctg
      781 aataatcaca gattatccta ttatatagaa aggctgcaat cgtttgacgg ttgcagcctt
      841 ttttacgtaa tatcctttat ctgcttattc cggtttctga gcgggcacat ttcctcccaa
      901 gaagaaatct tctatctttt gcttactttg aatgtctttc agccagttag ctgcagcatt
      961 ataataattc ttagtgccta aaacgagttc gcctcgttcg tttagtttcg cttcttcttc
     1021 cttatcttct ttcgggcgtg tttttaagaa tgcttccaga cagcgttcgg tatttttctt
     1081 gtctttcagc gaatagaaat aaatgaaagc catatcatag agcagcttat gattttgttt
     1141 atcgtatttc cggtagcgtt ctctgatcga ttcaatctgc tctttaggca tctgtgccat
     1201 tttgtagcag tcggtcatgc caatatagag ccgtaccata ttactgtctt tgggaatagt
     1261 cagatcaagc gctttttcga gatactctac gcctaacttc ttccatgaag tcttggcgca
     1321 ggcacggccg agataataaa gcaggtttac gttttcagga tcatgttttc gggcagcttc
     1381 cagaaagtca tgtgcttcgt aatatttctc ttccgcataa tagctgatac ccagataata
     1441 acaggtatga aaagaactgt cgccctggct caccaggtat tcataacgct ggatagcggt
     1501 aggataatcc ttattcagac aatatgccag tgcattctga cggttgacag cgatattggt
     1561 ggtatcaatc tgccgatatt tctccgtggc ttcaattgct tcgttgaaat aagaaccggc
     1621 tatgttcagt gcggcgagct tggcggcggc aagatagtcg tcggggtatt tctcctgtat
     1681 attatagtag caccccgtgg cggggagaag ctctcccata ccttcgaaac tttgggcctg
     1741 cagatgcaag gcgatggcgg aactgtcttt ctctgtcatc agactacttt ctcctaaagc
     1801 atcctgaaac ttctgtaaag agagcagtag tccgatgtat tgaatacgca catatttgtt
     1861 ttccggattc agatcaagtg cctgttcata atatttcagt gcctgttggt atttggccaa
     1921 tgaacggcag cattcggctg cttcgatata tgcacgggta ttggtagtgt cattggttat
     1981 gatttcctga taagtggaaa gtgcttctgt attcatccca agtccccgtt gtgctttacc
     2041 tttttgcaag agtaggggaa ttgtcggctt ttctttggca atgagagtga gagctgcctc
     2101 gtaatcgtag tttgctattg cttcctgaac atgattgatg cgctgcgcca tcaccagggt
     2161 ggtggtacaa aggaaggcga ggagaataat ccgtttcata aatatttatt ttagtgtgtc
     2221 gtaacaaaga cgcaagatgc taaaaaaata tcgtatttac gaattattcg tagccttttt
     2281 ggaatagatt aacggaatga taagagaaga taacataatg ataagtgcgg atattgcatc
     2341 taaaacagca atatccgcac ctaatatttt gtcccggcat tccgaatgga aacctgacag
     2401 gggcaccttg tccctacatt aagaatccta acaggatacc ggcagcaaca gccgaaccaa
     2461 ttacacctgc tacgttcgga cccatagcgt gcatcagcaa gtagttggta ggatcatatt
     2521 ccagacctac aacttgtgag atacgtgcag agtcgggaac ggcagataca cctgcattac
     2581 cgatcaacgg attgatctta ttgcccttct tgaggaagag gttgaagatc ttaacgaaga
     2641 tcacacctga tgcggtagca atgataaatg acagagcacc gagggcgaag atcttgatgg
     2701 agtcgagtgt caggaactca gaagcctgag tagaagcacc tacggtcaaa cctaacagga
     2761 tagtgattgt gtcaatcagc ggaccgctgg ctgtgttagc cagacgacgg gttacaccac
     2821 tttctttcaa caggttaccg aagaacagca tacccaacag aggcagaccg gaaggaacca
     2881 ggaagcaggt cagcaacaga ccgataatcg ggaaaatcac tttctctgta tgagaaacgg
     2941 cacgtggcgg tttcatacgg ataacgcgtt cgtgtttggt ggtgagcaga cgcatgatag
     3001 gcggctgtat caccggaact aacgccatat aggagtaggc ggataccgca atggctccca
     3061 ttaagttagg tgccagcttg gacgaaagga agatagccgt cggaccatct gctccaccga
     3121 tgataccgat agcaccggcc tgcatcgggt caaatcccat ttccaatgcg atcatgtatg
     3181 caccgaagat accaaactgg gcagctgcac caatcagcat cagcttcgga ttggagatca
     3241 gcgccgagaa gtctgtcatg gcaccgatac ctaagaagat gagcggtgga taccagccgg
     3301 acgttactcc ctgatacaat atgttcaata cagaaccttc ttcatatata ccgactttta
     3361 gtccggcgtc catattgaaa ggtatattac cgatcagaat accgaatccg ataggaatta
     3421 acaacatcgg ctcgaactct ttggctacag ccaaatagat gaagaacaag ccaacgagga
     3481 gcatcacgac atgaccaact gtcgcgttgg caaatcccgt gtaggtccag aagtcggcaa
     3541 ggttatttcc taaaaagtta ataaaatctc ccatattatt caataattac gaggtcatta
     3601 ccttcaagaa cagaatctcc cttgttgaca ttgatagcag tcactttacc gtctttgtct
     3661 gcattgatat tgttttccat cttcatggct tccaatataa tgatagtctg tcctcttttc
     3721 actgtatcac ctacatttac tttgatgtca aggataacgc ctggtagcgg ggacttaacg
     3781 cctgatttgc ctgtagaagg agcagccggt tttactaccg gtgtggtagg agcagccggt
     3841 gagttaggca taggacgtac tactaccggt ttgggagcag ttttcggttg tttctccatt
     3901 tctactttgt agtgcgtacc gttcacttct acatgagcga tattatcttc aatatctccg
     3961 atggttactt tgtatgagtt accgttgatt ttatatttat attctttcat cgttttatct
     4021 ttttagggaa tgatttactt tctgttagga ttttcacgca aggtgtaaat cttcgaactc
     4081 cacggtgaat aactgcgttt cacacgagtg atggtcagca ccgtttcttc tacgtcatgt
     4141 acctcatcct gcatttcgtg taatgccatt gaaatagcag cataaacttc acccggagcc
     4201 tgtgaaagct ctttcgcttc gactttatcg atgcctttag ccttcatcgc attgcgtttg
     4261 ctcaggttga cagcaacttt acctaccacc ttgaaggcaa tgaagagaag aatcaatccg
     4321 cagaatacta cgctcatggc tgaaatagac ataccgatac cgactgagtc atgttcttcg
     4381 aacttttcca tcttggcatt actgtcaagg gttttgttgt tcgtgctcgg ggttacgtat
     4441 ttgtctttgc ttccgccttt tacttcccag tctgctgctc cgtcgcttat gcgtgcataa
     4501 gactggttgg gacctaaagt tcctgctggt atgacaactt gatcaagtag ctttctgcct
     4561 gaatcaaaaa gaccgaccca attggctgtt tcgggattca gtttgaagct ggtatggaag
     4621 gttccacgat taggttcgcc atctgcccaa aataacgcat gttggcgtgg ctttactaat
     4681 gtaagtatgt caccttttgg gataaaatag gttactgtat cgcccggctg gctgcttact
     4741 ttcaataaac aagctgctaa gtctgcgcta ccgtatgatt tgttgaatat ttcaatccat
     4801 gcactgtgca agccgtagtc atcctgaaag ttgttttgat tgtctatcag gacttcattc
     4861 agcaatagct tattgttcga cttttgctct ccgcaggaag tcaatccaat cagcaacagc
     4921 agagaaagga atattccgat tttggttttg ttcataatcg ttcttgtttt aatgtgaaaa
     4981 gttggattac aacggaatat taccatgttt cttcgccgga ttagacagtt tcttagtctg
     5041 caactgctgc aaagcacgga tcacgcggaa acgtgtgtta cggggttcga tgacatcatc
     5101 gatgtaacca tacttagctg cattataagg gttggcaaac agttttgtat attctgcttc
     5161 tttttcggca aggaactgag ccggattttc ctggtctttg gcttcacgcg cgtacaatac
     5221 ttctactgca cctgcaccac ccattacagc gatttcagct gttggccaag catagttcat
     5281 gtcaccacga agttgtttac aactcatcac gatgtgagaa ccaccgtaag atttacgcaa
     5341 tgtgattgtt actttaggta cagtagcttc accgtaagca tacagcagtt tggcaccatg
     5401 aaggataacg ccattgtatt cctggcctgt tcccggaagg aatcccggta cgtctaccaa
     5461 cgacacgata gggatattga aagcatcgca gaaacgaacg aaacgtgcgc ctttgcgaga
     5521 tgcgttgctg tcgagtacac cggcaaggaa tttaggctga ttggctacaa tacctacaga
     5581 ctgaccgttg aaacgggcaa aaccgataat gatattcttg gcatagtctt tctggatttc
     5641 caggaattcg ccgttgtcta cgatagcgct gataacctcg tacatatcat acggtttgtt
     5701 ggggctgtcg gggataatat cgttcagtga atcttccaga cggtcgatcg gatctgtgca
     5761 atccacgtat ggagcttctt cgaggttgtt ttgaggaata tagctcagaa gtgtgcggat
     5821 caatgccaga ccttcttctt ctgttttggc agtgaaatga gtaacacccg atttggtaga
     5881 gtgaacgctt gctccgccga ggttttcctg gcttacgtct tcaccggtta cagttttcac
     5941 aactttcgga cctgtaagga acatataaga agtaccttcc atcatcagtg tgaagtctgt
     6001 cagtgccgga gaataaacag caccgccggc acaaggaccg aagataccgg aaatctgcgg
     6061 aatgacaccc gaagcgagga tattgcgctg gaagatttct gcgtaacctg ccaaagcgtt
     6121 aataccttcc tggatacgtg caccgcccga gtcattgata ccgatacaag gagcacccat
     6181 cttcattgcc tgatccataa tcttacagat cttcagtgac attgtttccg acagtgaacc
     6241 tgcagtaacg gtaaagtcct gtgcaaaaag atatacgaga cgaccttcga tagtaccgca
     6301 accggttact acaccatcac ccggatagtg ctttttctcc atgccgaagt tcgtgcatct
     6361 gtgctcaacg aacatgtcca tttcttcgaa gctaccttca tccaacagca tggctatacg
     6421 ctcacgcgct gtgtatttcc ctttttcatg ttgcttcgca attgcttttt cgccgcctcc
     6481 gatgcgtgca acggcacggc gttcaataag ctctttaatt ttttcaagtt gattactcat
     6541 gagttcttgt tgttttttta gagtttatga ttagtgttcg cagagttctg tcagcacacc
     6601 cagtgttgat ttcgggtgaa ggaaagcgat gttcagacct tcggcacctt tgcggggagc
     6661 cttgtcaatc agacggattt ctttgctttc tgcttctgcc aaagcattag ctacgccatc
     6721 ttctacggca aatgctacgt gatgaacacc tgcacctttg ttttcaataa atttagcaat
     6781 ggtgctttcg gggcaagtcg gctccaacaa ttcgattttt gtttcaccta cctttaaaaa
     6841 agcggttctt actttttgat cttctactgt ttcgatgttg taacatttca gacccaatac
     6901 gttttcgtag taaggaaggg cttcttcgat gctttttaca gcaatgccca gatgct
//
