LOCUS       gnlProkkaBIONAIJF_200  13574 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BIONAIJF_200.
ACCESSION   gnlProkkaBIONAIJF_200
VERSION     gnlProkkaBIONAIJF_200
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 16:39:48
            Original ID  :: gnl|Prokka|BIONAIJF_200
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 304
            Orig. end    :: 13878
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..13574
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:8574]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..8574
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..13574
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..13574
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             243..611
                     /locus_tag="ctg200_2"
                     /transl_table=11
                     /translation="MDAVILQENIEGLLNLVRMLLPGGGSAGCVYLDDLSVLQRSIHKQ
                     INDLYSQRGKTPEQDATLCLAILQGYNVSMYANPEDEDRKRSVLQRSLTLLDVLPPSLL
                     KQQLSAVCHGMQELCETN"
     CDS             complement(711..1373)
                     /locus_tag="ctg200_3"
                     /transl_table=11
                     /translation="MEDGREGRKGVGTRLHDLSHHVDLDSTDITQSQTDVGSRVGSIVQ
                     TVIDLIQGALQIVVGGGDCHTAKVDRPDLLNIDGAFRRDLLADRVLASTPDIDDDFITG
                     TQTVIGRSGKVDTRLERQVAGIEDVAPENLVLTGQIFRSRMILQHVGSILLSLFTKAGL
                     VDIRTRGLGGVYIVLLLIELLHTHSLFLGHTFTRKLGSKLLLFLALPDAVLYIFQYL"
     CDS             1395..3038
                     /gene_functions="transport (smcogs)
                     SMCOG1186:polysaccharide export protein (Score: 70.6;
                     E-value: 1.6e-21)"
                     /gene_kind="transport"
                     /locus_tag="ctg200_4"
                     /transl_table=11
                     /translation="MSDIGSLRNVQLVRNGKNIATIDVYEFIMKGNTQDDIRLQEGDVV
                     IVPAYDVLVKISGKVKRPMRFEMKKDENLATLIKYAGGFEADAYTRSLRVVRQNGEEYE
                     VNTVKDMDYSIYTMRNGDVVTAEAILNRFTNKLEIRGAVYRPGIYQLSGKLNTIRELVN
                     EAQGLTGDAFLNRAVLYRQREDLTSEVVQIDIKSIMDGTSPNLALMKNDILYIPSIHDL
                     EDRGNVTVHGEVAQPDSYPYADNMTLEDLIIQAGGLKEAASTVRIDVSRRIKNPRSIAD
                     NDTIGQMYTFSLKDGFVIDGQPGFILQPYDEVYVRRSPGYQAQQNVVIDGEILFGGNYA
                     MTNREERLSDLVNKAGGPTSLAYLRGAKLTRVASAGEKKRMGDVIRLMSRQLGEAMIDS
                     LGIRVEDTFTVGIDLEKALSNPKSNADLVLREGDVISIPKNTNTVTINGAVMVPNTVSY
                     MKGKNVDYYLNQAGGCSDNARKSKKFIVYMNGQVTKVKGSGKKQIEPGCEIIVPSKAKK
                     KGNIANILGYATSFSSLGMMIASIANLIKK"
     CDS             3049..4197
                     /locus_tag="ctg200_5"
                     /transl_table=11
                     /translation="MIEKQSYQEPFSEVDKRKDDEMEIDLMAIFYKIITVRGVLCKAAG
                     VGVIIAIVVALSIPKQYTVKVTLSPEMGNAKGSNGLAGLAASFLGDGALIGESTDALNA
                     SLSADIVSSTPFLLELLEMEVSVSKKDDKMTLGSYLDEESSPWWDYLIGFPGIVIGGVK
                     SLFGEDTVPASGGRQGTIELTKEVNEKINFLKKKISASIDKKTAITSITVTLQNPQITA
                     VIADSVVHKLQEYIIGYRTSKVKEDCAYLERLFKERQQEYYAAQRKYADYVDAHDNVVL
                     QSVRAEQERLQNDMSLAYQIYSQVANQLQVSRAKVQEEKPVFAVVEPAVVPLKPSGMGL
                     KIYILLFVFLSVSGTLVWVLFGKSLLASLRKELKNKEIEQDK"
     CDS             4363..4980
                     /locus_tag="ctg200_6"
                     /transl_table=11
                     /translation="MIVRSIDTLLSCGIERIVIGTGYKQEVYEELKTDYPMLETCFSPR
                     YAETNSMYTLYNTREILGNDDFLLLESDLIFEKQAIMSLLECPAADAMLITPVTKFQDQ
                     YYVEHDDNFRLSSCSVNKNKLNAKGELVGIHKLSGSFYKIMCADYASIVDEQPNLGYEY
                     ELLRISCSQSPVYVHKVEGLKWYEIDDISDLEYAEKYIVPYC"
     CDS             5001..6302
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 209;
                     E-value: 1.4e-63)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg200_7"
                     /sec_met_domain="CTP_transf_like (E-value: 4e-13, bitscore:
                     40.6, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 1.7e-121, bitscore:
                     395.5, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 2.1e-63, bitscore:
                     204.8, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKTVYIGMTADIMHPGLIRIINEATKYGDVIIGLLTDKAIAEHK
                     RLPYLTYEQRKEVVQNIKGVCKVVPQEEWSYVENLKRIKPDYIIHGDDWKTGPLREERV
                     RVFEVMNEQGGKVIEIPYTLGINSSSLDKDIKAIGTTPDVRLKSLRRLINAKPVVRILE
                     AHDGLCGLIIENQEILKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAIIIEDKVGLKKNSLFGTDVIQTQDT
                     IEGFCNKIKAGKASQITDDFMIIARIESLIAGKPVSDALERAFAYVQAGADGIMIHSKN
                     KSGEDIKEFCLAFRKQYAHVPIVVVPTTYDHIYESELCDWGVNIIIYANHMLRAAYPAM
                     MNVAKTILENERALEVRELCMPIKEILELIPGTK"
     CDS             6319..7443
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 58.1;
                     E-value: 1.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg200_8"
                     /sec_met_domain="TPP_enzyme_N (E-value: 2.2e-20, bitscore:
                     63.7, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.9e-20, bitscore:
                     64.0, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSVEKVYETFLSHDVDFFTGVPDSLLKNICAYITDHTSREKHII
                     AANEGAAVGIASGYYMASGKVPVVYMQNSGLGNTVNPLLSLADEQVYSFPMLLMIGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMRIPYIILDSNENDAFIQLHDIIQSAKTKNIPHAII
                     IRKDVFGKYKLQKEFGCNYPLSREDALQIVVDYLPENSVVVSTTGKLSRELFEYREMKE
                     QGHEHDFLTVGSMGHSSSISLGIAIAKQDRPVYCLDGDGAFIMHLGAISNIGDLSPKNY
                     YHILFNNGAHESVGGQPTLGFSLDIPAIVRGSGYKHTYTVCTKVEIEEAMKQLPKLCGP
                     VLLEIKVKIDSRENLGRPTTTPIENKEHFMDFLK"
     CDS             7453..8574
                     /NRPS_PKS="Domain: Aminotran_5 (30-366). E-value: 2.9e-34.
                     Score: 110.4. Matches aSDomain:
                     nrpspksdomains_ctg200_9_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 76.3; E-value:
                     3.6e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg200_9"
                     /sec_met_domain="Aminotran_5 (E-value: 3.1e-31, bitscore:
                     99.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIERKILLNPGPATTTDSVKMAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGNLEKHTAVLFCGSGTINIDICLNSLLPEDKRVLIVNNGAYSTRAVEVCQYY
                     GLPHINLEFSVYERPDLSVVENVLQENPDIAMVYTTHHETGTGILNPIREMGAIAHKYQ
                     AKFVVDTTSSLGMIPFDIEKDNVDFCMASAQKGLQAMTGLSFIIGNEEQIKFSKKYPKR
                     SYYCNLYLQYENFERTGEMHFTPPVQTIYATRQALNEYFEVGEEAKFARHKRVFEAIHS
                     GINEIGLKSVIKREWQSGLVVSVQYPEDPNWDFEKIHDYCYERGFTIYPGKISTEDTFR
                     ICALGEIEVDDIENFFKVLKAALNHYNVTLPMS"
     aSDomain        7543..8550
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg200_9_Aminotran_5.1"
                     /evalue="2.90E-34"
                     /label="ctg200_9_Aminotran_5.1"
                     /locus_tag="ctg200_9"
                     /protein_end="366"
                     /protein_start="30"
                     /score="110.4"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGNLEKHTAVLFCGSGTINIDICL
                     NSLLPEDKRVLIVNNGAYSTRAVEVCQYYGLPHINLEFSVYERPDLSVVENVLQENPDI
                     AMVYTTHHETGTGILNPIREMGAIAHKYQAKFVVDTTSSLGMIPFDIEKDNVDFCMASA
                     QKGLQAMTGLSFIIGNEEQIKFSKKYPKRSYYCNLYLQYENFERTGEMHFTPPVQTIYA
                     TRQALNEYFEVGEEAKFARHKRVFEAIHSGINEIGLKSVIKREWQSGLVVSVQYPEDPN
                     WDFEKIHDYCYERGFTIYPGKISTEDTFRICALGEIEVDDIENFFKVLKAALNHY"
     CDS             8571..9044
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1156:Chloramphenicol acetyltransferase (Score: 73.7;
                     E-value: 2.7e-22)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg200_10"
                     /transl_table=11
                     /translation="MIAEILLKIRHFYFSLRGIHFHPESVISGVPIRSFRLVGKYSNIY
                     LGKNAEIRSGCYLIARDKIIIGENSTLAYGTTILTTADPNGPYNALAVLYPYKKAPVVI
                     GDNVWIGANVTILPGVTIGDYVVVAAGAVVTKDVPSGVLVAGVPAIVKKRFNK"
     CDS             9041..10486
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg200_11"
                     /sec_met_domain="Polysacc_synt (E-value: 1.7e-32, bitscore:
                     103.7, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEKTSLKNKAICGIFWSAVERFLSQGIQFVVTVIMARFLLPSDY
                     GMVGMLAIVLQLSQTLMDSGFTNALIRYKERTEIDYSTVFFFNILASLVIYFLIFFLAP
                     YISWFYKIGQLVEITRILSIVIVLNSFAIIPKTKLIIAVDFKTQSKVSFIAALVSGIIG
                     IWAAYLDFGVWALVGQTLVNGLLIVVLYAYFCRWKPLWIFSLKSFRLLFAFGSKLVLAN
                     ILNTLYKNLYAIVIGKKFSSVDLGFYTRAEQFAQFPSYNLSAIISRVTYPILSLVQNDN
                     DRLSALYRKYVRLSSAFIFPLMILLIVIAQPLVLLLLTEKWIGVVVLLQILCLDWMFDH
                     LSSINLNLLYVKGRSDLALRLEVIKKVLATGILLLSIPWGIVGMCWGRVMYSLIAVYLN
                     TYYTESLIKLSLIRQMRDILPSFFLALIMGGGTWYMILLLEGEIVQIVVGSVIALLLYG
                     TLLYCFSRSLFDEIWAVILKIKR"
     CDS             10486..11208
                     /locus_tag="ctg200_12"
                     /transl_table=11
                     /translation="MDKLSLIISKIRRNHWYRTYMRPIYLFVYGNRKLKNRNRIFLQYS
                     QELLTNFHQAMNEVGIPYWLEFGTLLGAVREHSFISHDFDLDVGLFKEDYSDIIREVLK
                     KYGFKRIRCITALDFIDAYEETYLYKGVTIDLFLFSREKNCDGVYCYDFVPVYSDIKRT
                     KKGDNAIRRIHFPRIDLIEYSFIGINTFVPENYEEHLCMHYGKDYMIPNPHWKDSDAQH
                     TDIFYGREIRIELFDKSK"
     CDS             11222..12235
                     /locus_tag="ctg200_13"
                     /transl_table=11
                     /translation="MFYLFFLLIVLFLLYIQSRKRQYSTVAGICVPIIIIFVVICRYRV
                     GYDYLTYYTLIENEEEAQILYLFSPLSIVWAEIALYFESPQMLFVLFGLPTFALLIFTL
                     KKYSKDYALSVTILICFFFFTSLSIIRQALALAICFYGYRFIVKRSLIKYILCILIAAL
                     FHPSAGVAIIIYWLPFLKFRLVVLLSIISIVAKPLFFYMLNILGVYTSYLDGKMDIEGG
                     KYTQLIYFLLFIICFVLGSKNIDKNKCNLFNVLLLSFVFVSFLGAHLGGRIAFYFNIYY
                     CLLIPKVVKGLDGQLREQVKLLSYFLCISYFLLYLFVPFIKGVPSYYIPYKFIFFK"
     CDS             12189..13199
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1123:polyprenol-monophosphomannose synthase ppm1
                     (Score: 57.1; E-value: 3.5e-17)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg200_14"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.3e-42,
                     bitscore: 135.5, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYPHIISHISLYFSNSKMLISIIVPCYNSERYLSDCLLSILNQTY
                     VNWEVICIDDGSQDNTLSILNHYATLDERFKIISQSNAGVSVARNKALSQCQGEYICFV
                     DSDDLIEPNFLITLLQLISHQADLAICGFTRSMVFCMDDAQGYIEMSGKRCIERIILDK
                     SFNPQICCMLFRHNIIKEKQLEFVIGCTRGEDREFILKYLVYSNKVCYTNNLLYHYRVN
                     DQSAMAIFSIKSLTSIEASQRTVNYYKKVNYSVRGLEFEFSRTLWKFMILAILSGQNDL
                     YQIIREKYDLKRQMEHLYSHPGLSEKLTARLFVWNEALFKWVFSIIRYLYKKRWR"
ORIGIN
        1 ggcccgtttc cgtttcatgc ttgattattc tgaagaatcg atcagcatga acagtgcccc
       61 tttggcccgt ggcgaaaagg tccgcgtgat taaaggcccc ctgacgggtc ttgttggcga
      121 actggttcat gtggatggca agagtaagat cgccgttcgc ctgaatatgc tgggatgtgc
      181 ctgtgtggat atgcctgtcg gttatgtaga gcctattatg gcggctgttt aaggtgaggg
      241 atatggatgc tgttatttta caggaaaaca tagagggttt attaaatttg gtacgcatgc
      301 tgcttcctgg tggggggagt gcaggctgcg tgtatctgga tgacctttcc gtcttacaac
      361 gatccatcca caaacagata aacgatttat attcacaacg ggggaagaca ccggagcagg
      421 atgctaccct gtgtttggcc atccttcagg gatataatgt gtcgatgtac gccaatccgg
      481 aagacgagga tagaaaacgg tctgtcttgc agcgttctct gaccctcctt gatgttcttc
      541 ccccttctct tcttaaacaa cagttgtctg cggtctgtca cggtatgcag gaactgtgtg
      601 aaaccaacta acttttttat ttgtcaatga aaatacaacg actttttagc attctacttt
      661 tttgttctat gctgggaagt ggctcgctta tggcccagtc catgagtgac tcacaggtac
      721 tggaatatgt aaaggacggc atccggcaag gcaaggaaca gaagcagctt gcttccgagc
      781 ttgcgcgtaa aggtgtgacc aaggaacagg ctatgcgtgt gaagcagctc tatgagcagc
      841 agaacaatgt aaacacctcc caatccacgg gtacggatat caacgagtcc cgccttcgtg
      901 aagagactaa ggagaatact tccgacatgc tggaggatca tccgactacg gaagatttgg
      961 cccgtgagga ccaggttttc gggcgcaaca tcttcaatac ccgcaacctg acgttcgagc
     1021 ctagtgtcaa ccttgccact cctgccaatt accgtctggg tcccggtgat gaagtcatca
     1081 tcgatatctg gggtgctagc cagaacacga tccgccagca gatctctcct gaaggcacca
     1141 tcaatattca gaagatcggg cctgtcaacc ttagcggtat gacagtctcc gccgccaacg
     1201 actatctgaa gggcgccctg aataagatct ataacggtct gaacaatact accgacccta
     1261 cttccgacat ccgtctgact ttgggtaata tccgtactat ccagatcaac gtgatgggag
     1321 aggtcgtgca gccgggtacc tacgcccttt cttccttctc taccgtcttc cacgcgcttt
     1381 accgtgcagg cggcgtgagt gatatcggca gcctccgaaa cgttcagttg gtacgcaacg
     1441 gaaagaatat cgccaccatc gatgtttacg aatttatcat gaaaggcaac acgcaggatg
     1501 acatccgcct tcaggaaggt gacgtggtta tcgtccccgc ctacgatgtc ttggtaaaga
     1561 tcagcggcaa ggtaaaacgt cctatgcgtt tcgaaatgaa gaaagacgag aaccttgcca
     1621 ctttaatcaa atatgccggc ggctttgaag ccgatgccta cacccgttca ttgcgtgttg
     1681 tccgtcagaa tggtgaggaa tatgaggtca acacggtcaa ggatatggac tacagcatct
     1741 acacaatgcg taacggcgac gttgtaacag ccgaagccat ccttaaccgc ttcacaaaca
     1801 aactggaaat ccgtggcgct gtctatcgtc ccggcatcta ccagctcagc ggtaaactga
     1861 atacgatccg cgaacttgtc aatgaagccc agggtctgac cggtgacgct ttcctgaatc
     1921 gtgccgtcct ttatcgccag cgtgaggatt tgaccagtga agttgttcag atagacatca
     1981 agtctatcat ggacggtact tccccgaacc tcgccctgat gaagaatgac atcctttaca
     2041 ttccgagcat tcacgatctg gaagatcgtg gcaatgtcac agttcacggt gaagtcgcgc
     2101 aaccggattc ctatccctat gctgataaca tgacacttga ggatctgatc atccaggccg
     2161 gtggcttgaa agaagccgcc tctactgtcc gcattgatgt gtcgagacga atcaagaatc
     2221 cccgtagtat cgcggataat gatactatcg gtcagatgta taccttctct ttaaaggatg
     2281 gttttgtcat cgacggtcag cccggtttta tccttcagcc ttatgacgaa gtttatgtcc
     2341 gtcgcagtcc gggttatcag gcgcaacaga atgtagtcat tgatggtgaa attctgtttg
     2401 gaggaaatta tgccatgacc aaccgtgagg aacgtctttc cgatctggtg aataaagcgg
     2461 gtggtcctac gagtcttgcc tatctccgtg gtgcaaagct gacccgcgtg gcaagtgccg
     2521 gtgagaagaa acgtatgggt gatgtgattc gccttatgag caggcaattg ggcgaagcca
     2581 tgattgactc tttgggtatt cgtgttgaag atacttttac ggttggaata gatttggaaa
     2641 aagccttgag caatcctaaa agtaatgccg atcttgtctt acgtgaaggt gatgtgatct
     2701 ccatacctaa gaatactaat actgtaacta tcaatggtgc tgtcatggtt ccgaatacgg
     2761 tttcttatat gaagggaaag aatgtggatt attacctgaa tcaggccggt ggctgttctg
     2821 ataatgcaag gaagagtaag aagttcattg tttacatgaa tggtcaggtg acgaaggtta
     2881 agggtagtgg taagaaacag attgagccgg ggtgtgagat tattgtgcct agtaaggcta
     2941 agaagaaagg gaacatagct aatattttag gttatgctac ttccttcagt tctttgggaa
     3001 tgatgattgc ttctattgct aatttgataa agaaataaat gacctattat gattgaaaag
     3061 caatcttatc aagaaccgtt ttctgaagtg gataaaagga aagatgatga aatggaaata
     3121 gacttgatgg ctatttttta taagattatt actgttcgtg gagtactatg caaagctgct
     3181 ggagtgggag taataattgc tatagtggtg gcattgagca ttcccaagca atatacagta
     3241 aaggttaccc tttctcctga aatgggtaat gcgaagggaa gtaatggttt agccggactt
     3301 gctgcctcgt ttttgggaga tggggctcta ataggggaaa gcacagatgc cttgaacgct
     3361 tctctttcag cagatattgt ttcttcaact ccttttttat tggaactttt agaaatggaa
     3421 gtctccgttt ctaaaaagga tgataaaatg actttggggt cttatctcga tgaagagtca
     3481 tctccttggt gggattatct tataggtttt ccgggcatag taataggagg agttaaatca
     3541 ttattcggag aagatacggt tcctgccagt ggagggcggc aaggaactat cgaattgacg
     3601 aaagaagtaa atgagaagat taattttctt aaaaagaaga ttagtgcatc cattgataag
     3661 aaaaccgcca taacgagcat tacggtcacc ttacaaaatc cgcagatcac tgcggttata
     3721 gcagattctg tcgttcataa attacaggaa tatattattg ggtatcgaac ttcaaaagta
     3781 aaagaagact gtgcgtattt ggaacggttg tttaaagaac gtcagcagga atattatgct
     3841 gctcaaagaa aatatgcgga ttatgtagac gcccatgata atgtggttct tcaaagtgta
     3901 cgtgctgagc aggagcgttt acagaacgac atgagtttgg cttatcagat atatagtcag
     3961 gttgctaatc agttgcaggt atccagagcc aaagtacagg aggaaaagcc agtctttgca
     4021 gtagttgagc ctgccgttgt acctttgaaa ccttcaggta tgggactcaa aatttatatt
     4081 ttattatttg tgttcctgtc cgtatctggt actctcgtgt gggtgctttt tggcaaaagt
     4141 ctgctggcta gcttgagaaa agaactgaaa aataaggaga tagaacagga taaatgatgc
     4201 taaaacgtcg tttacaacat tcttacatca attaaattca cttactatat tattaaacga
     4261 atgatacgta cagctatgat aatggctgcg ggcatgggaa cccgttttgg ccaatataca
     4321 gaattgatac ctaaagggtt cgtcaaagtg ggaggtattc caatgatagt tcgttctatt
     4381 gatactttgt tatcatgtgg cattgagcga attgtaatcg gtacaggata taaacaagaa
     4441 gtttatgaag aattgaagac agactatcct atgcttgaaa cctgttttag tcctcgttat
     4501 gctgaaacaa atagtatgta tactttatat aatacgcggg agattctggg caatgatgat
     4561 tttttattgt tagagtcaga tttgattttt gagaaacaag caataatgag cttattggag
     4621 tgtcctgcgg cagatgcaat gctaataact ccagtgacaa agtttcaaga tcagtattat
     4681 gtggaacatg atgataattt tcgcctttct tcatgttctg tcaataagaa taaactaaac
     4741 gctaaaggcg aattggtcgg catccataaa ctatccggtt ccttttacaa aataatgtgt
     4801 gctgattatg cgagtattgt tgatgaacaa cccaatttgg gatatgagta tgaattacta
     4861 cgaatatctt gttctcaatc tcctgtttat gtgcataaag tagaagggct gaaatggtat
     4921 gaaatcgatg acatatcaga tttggaatat gcagagaaat atattgttcc gtattgttaa
     4981 ttaaaataat atatcaaatt atgagtaaga cagtttatat tgggatgact gcggatatta
     5041 tgcatccagg acttatccgc attattaatg aggctactaa atacggtgat gttataattg
     5101 gtcttcttac agataaagct attgcggagc ataaacgcct tccttatctg acctatgaac
     5161 aacgaaaaga ggtagttcag aatattaaag gcgtatgtaa agttgttcct caagaagaat
     5221 ggagctatgt tgagaatctc aaacgcatta aacctgacta tataattcat ggtgacgact
     5281 ggaaaaccgg gcctttacgt gaagaacgtg tgcgcgtatt tgaagtgatg aatgaacagg
     5341 gaggaaaggt tatcgaaatt ccatatactt tgggaattaa ttcatcttct ttggataaag
     5401 atattaaggc tataggtaca acccctgatg tacgtttaaa atcacttcgt cgtttgataa
     5461 atgccaaacc cgtagttcgt attttggaag ctcatgatgg actatgtggc ttgataattg
     5521 aaaatcagga aatattaaaa ggggataaga gggaagtatt tgacggaatg tggtctagta
     5581 gccttacgga ttcaaccagt aaaggtaaac ctgatattga ggcggttgat ttaactaccc
     5641 gattgcaaga tttgaataat attctggagt gtactactaa acctattatt tttgatggag
     5701 atacaggtgg taaaattgaa catttcgtat ttacagtacg cactttagaa cgtcatggaa
     5761 tttcagctat aataattgaa gataaagttg gattaaaaaa gaattcattg tttggtacag
     5821 atgttattca aactcaagat acaatcgaag gtttctgtaa taagatcaaa gcaggaaagg
     5881 catctcagat tactgatgat tttatgatta tcgctcgtat tgagagcctt atagcgggaa
     5941 agccggtaag tgatgcttta gagagagctt ttgcatatgt ccaagcggga gcggacggta
     6001 ttatgataca tagcaaaaat aagtcgggtg aagatataaa agaattttgt ttggcgtttc
     6061 gaaaacagta tgctcatgta cctatcgtgg ttgtgcctac tacatatgat catatttatg
     6121 agtctgaatt gtgtgattgg ggtgtgaata taattattta tgctaatcat atgcttcgtg
     6181 ccgcttatcc tgctatgatg aatgtcgcaa agacaatatt agagaatgaa cgtgctttag
     6241 aggtgcgcga actgtgtatg cccataaaag aaatattgga actgattcca ggtactaaat
     6301 aaaaagagga atatcattat gttaagcgta gaaaaagttt atgaaacgtt tctgagtcat
     6361 gacgttgatt tttttacagg agttccagac tcattactaa aaaatatttg tgcctatatc
     6421 acagaccata cttcacgtga gaaacatatc atagctgcta atgaaggtgc ggctgtagga
     6481 attgcttcag gttattatat ggcatcaggt aaggttcctg tggtttatat gcaaaattcc
     6541 ggcttgggta atactgttaa tcctctgctg tctcttgcag atgaacaagt atacagtttt
     6601 ccaatgcttt tgatgattgg ctggcgtggt gaaccgggaa caaaagatga acctcagcat
     6661 aaaaaacagg gtgaggtaac tttagatttg ttgaaagcaa tgcggattcc ttatataatt
     6721 cttgattcaa atgagaatga tgcatttatt cagctccatg atatcataca atcagcaaaa
     6781 actaagaata ttcctcatgc aataattatt cggaaagatg tttttggcaa atataaactt
     6841 caaaaagagt ttggatgtaa ttatccttta tctcgtgagg atgctttgca aatagttgtg
     6901 gattatctgc ctgaaaattc agtggtcgtt tctactactg gcaagctttc tcgtgagtta
     6961 tttgaatatc gagaaatgaa agagcaaggt catgaacatg attttctcac ggtaggctct
     7021 atgggacata gttcatcaat ctctttgggt attgctatag caaagcaaga tagacctgtt
     7081 tattgcttgg atggtgatgg agcatttatt atgcatttag gggcaattag taacattggc
     7141 gatttatctc ctaaaaacta ttatcatatt ttgtttaaca atggtgctca cgagtctgta
     7201 ggaggacagc ctactttagg atttagttta gatattccgg ctattgtccg ggggagcgga
     7261 tataagcata cttatactgt ttgtacaaaa gttgaaattg aagaagcaat gaagcagctt
     7321 ccaaagttat gtggtcctgt tttgttggaa ataaaggtta aaatagattc tcgggagaat
     7381 ttgggacgtc ccacgactac tcctatagag aataaggagc attttatgga ttttttaaaa
     7441 taaatatatt cgatgaaaat agaaagaaaa atattattaa atcctgggcc tgccactact
     7501 actgacagtg taaaaatggc acaagttgtt ccggatattt gtcctcgcga gaaagaattc
     7561 gcaggtatga tgaaacaact tcgggacgat ttggtcagag ttgctcatgg gaatttggaa
     7621 aaacataccg ctgtattatt ttgtggctca ggaaccatca atattgatat atgtctaaat
     7681 tctcttcttc ctgaagacaa aagggtgctt atagtgaata atggggcata tagtacccgt
     7741 gctgtagaag tgtgtcaata ttatggtttg cctcatatta atttggaatt ttccgtttat
     7801 gaacgtcctg atttatcggt ggtggaaaat gtgttgcaag aaaatcctga tatagctatg
     7861 gtttatacta ctcatcacga gacgggtaca ggaattctga accctattcg cgagatgggc
     7921 gctatagctc ataaatatca ggctaaattt gtcgtcgata ctacttcttc tttgggcatg
     7981 attccttttg atatagagaa agataatgtg gatttttgta tggcatctgc tcaaaaagga
     8041 cttcaagcga tgacaggtct ttcttttatt ataggcaatg aggaacagat taagttttca
     8101 aaaaaatatc ccaagcgttc ttactattgt aatctttatt tgcagtatga gaattttgag
     8161 agaacaggtg aaatgcattt tactccaccg gtacaaacta tttacgccac tcgtcaggca
     8221 ttaaatgaat attttgaagt gggcgaagaa gctaagtttg cccgtcacaa aagagttttt
     8281 gaggctattc attcaggtat aaatgaaatt ggtcttaaaa gtgtaataaa gcgtgaatgg
     8341 cagtcaggac tggttgtctc agttcaatac ccagaagatc ccaactggga ttttgagaaa
     8401 atacatgatt attgttatga gcgtggattt acgatttatc caggtaagat atcaactgag
     8461 gatactttta gaatctgtgc tttaggagag atagaagtag atgatattga gaacttcttt
     8521 aaagtactaa aagctgcatt aaatcattat aacgtcacat taccgatgtc atgattgcag
     8581 aaatattatt gaagattcgt catttttatt tttcccttcg tggtatacac ttccatccgg
     8641 aaagtgttat ttctggagtg cctattcgta gctttcgctt agtaggcaaa tatagcaata
     8701 tctacttagg taaaaatgct gaaatacgtt caggctgtta tctgattgct cgtgataaaa
     8761 tcattattgg agaaaactca acactcgcct atggaacgac tatattgaca actgctgatc
     8821 ctaatggacc gtataatgca ttagcagttc tttatcctta taaaaaggca ccggtagtta
     8881 taggagataa tgtttggatt ggggctaatg ttaccatcct tcctggagta actattggtg
     8941 attacgttgt agtagcagct ggagctgttg taacaaaaga tgtaccttca ggagtattgg
     9001 tagctggtgt accagctatt gtgaaaaaac gttttaataa atgaaagaaa aaacttcttt
     9061 aaagaacaaa gccatatgtg gaatcttttg gagtgcagtt gaacgatttt tatcacaggg
     9121 gatacagttt gtagttacag tcattatggc gcgttttcta ttgccatctg attatggaat
     9181 ggtaggaatg ttagcaatag ttcttcaatt atctcaaacg ttaatggata gtggttttac
     9241 taatgccctt attcgttata aggaacgtac tgaaattgat tattcaacag ttttcttttt
     9301 taatatatta gcatcattag ttatttattt tctaattttt tttctagctc cttatatatc
     9361 gtggttttat aaaataggtc aattagttga gattactaga attttatcta ttgttattgt
     9421 tttaaattcc tttgcgatta ttcctaaaac taaattaata atagctgtag attttaaaac
     9481 tcaatcaaaa gtctcattta ttgcagcttt ggtttcaggt attattggca tttgggctgc
     9541 atatttagat tttggagttt gggctttagt tggacaaact ttagtaaacg gattgttgat
     9601 tgtagttttg tatgcatatt tttgtagatg gaaaccattg tggatatttt cattgaaatc
     9661 gtttcgttta ttatttgctt ttgggtcaaa attagtatta gctaatattc ttaatacttt
     9721 atataaaaat ctgtatgcta tagttatagg aaagaagttt tcgtcggttg atttaggatt
     9781 ttatactcgt gcggaacaat ttgctcagtt tccttcatat aatttgagtg caataatttc
     9841 tagagtgaca taccccatat tgagccttgt gcaaaatgat aatgatagat tatctgcttt
     9901 atataggaaa tatgtccgtt tatcttctgc ttttatcttt ccattgatga ttttgttaat
     9961 tgtcatagct cagccactgg tattattgct tttgacagaa aaatggatag gagtagttgt
    10021 attgcttcaa atattgtgtt tagattggat gtttgatcat ttgagtagta taaatttgaa
    10081 tttactatat gtaaagggac gttcagactt ggctttacgt ttagaagtta taaaaaaagt
    10141 gttagcgacg gggatccttc ttctatctat tccttggggg attgtaggta tgtgttgggg
    10201 gcgtgttatg tattcattga ttgctgtgta tttgaatact tattatacag aatcattgat
    10261 taaattaagt cttattagac agatgagaga tatactacca agcttttttt tagcattaat
    10321 aatgggagga ggtacttggt atatgatttt attgttagag ggtgaaattg ttcaaattgt
    10381 agtaggaagt gttattgctt tattgctata tggaacgttg ttgtattgct tttctcgatc
    10441 cttgtttgat gaaatatggg cggttatatt aaaaataaaa aggtaatgga taaactgagt
    10501 ttgattatta gtaaaatacg acggaatcat tggtatagaa cctatatgcg tcctatatat
    10561 ttatttgttt atggcaatag aaaattgaag aatagaaata gaatattttt gcagtattca
    10621 caagagctgc taactaactt tcatcaagca atgaatgaag tgggaattcc ttattggtta
    10681 gaatttggaa ctcttttagg tgctgttaga gaacacagtt ttataagtca tgattttgat
    10741 cttgatgtgg gcctttttaa ggaagattat tcagatataa taagagaggt cttaaaaaaa
    10801 tatggtttca aaagaataag gtgtataacc gcccttgatt ttattgatgc ttatgaggaa
    10861 acttatcttt ataaaggtgt gacaattgat cttttccttt tttcaagaga gaaaaattgt
    10921 gatggggtct attgttatga ttttgttcca gtatattcag atataaagag aactaaaaaa
    10981 ggtgataatg caatccggag aattcatttt cctagaattg atttgattga atattcattt
    11041 attggaatta atacttttgt tcctgaaaat tatgaggaac atctttgtat gcattatggt
    11101 aaagactata tgattccaaa tcctcattgg aaagattcag atgcgcaaca tactgatatc
    11161 ttttatggta gagagattag aattgaattg tttgataaaa gtaaataata gtagataata
    11221 catgttctat ctattttttt tactaattgt gctatttttg ttatatatac aaagtaggaa
    11281 aagacaatat tctactgtag cgggaatttg tgttcctatt attataattt ttgttgtaat
    11341 ttgtcgttat agagtggggt atgactattt aacgtattat acattaattg aaaatgaaga
    11401 agaggcacag attttatact tattttctcc tttaagcatt gtttgggcag agattgcttt
    11461 atattttgaa tctccacaga tgttatttgt tctttttggg cttccaacat ttgctcttct
    11521 aatatttact ttaaaaaaat attcaaaaga ttatgcgtta tcagtaacta ttttaatttg
    11581 tttcttcttt tttacatcac tcagtattat tcgacaagct ttggctttgg ctatttgttt
    11641 ttatggttat cgttttatag taaaacgttc actaattaaa tatatattat gtatattgat
    11701 tgcagcttta ttccatcctt ctgcaggagt agcgattatt atatattggt tgccatttct
    11761 aaagtttaga ttagttgtat tattatctat aatatcaata gtagccaaac ctttattttt
    11821 ttatatgcta aatatattag gggtgtatac ctcttatctt gatggcaaga tggatattga
    11881 aggtgggaaa tatactcaat taatatactt tttgttgttt attatttgtt ttgttttggg
    11941 aagtaaaaac atagataaaa acaagtgtaa tttatttaat gttttattgt taagttttgt
    12001 tttcgtatct tttttaggag ctcatttagg cgggagaata gcgttttatt ttaatatata
    12061 ttattgtttg ttgattccta aagtggttaa aggattggat ggacaactaa gagagcaagt
    12121 gaagcttctt tcttattttc tatgtatttc ttatttctta ttgtacctat tcgttccatt
    12181 cataaaaggt gtaccctcat attatatccc atataagttt atatttttca aatagtaaaa
    12241 tgttgatatc tataattgtc ccttgttata attctgaaag atatttgtct gattgtttat
    12301 tgtctatatt gaatcaaacc tatgttaatt gggaggtgat ttgtatagat gatggaagtc
    12361 aagataatac attatctatt ttaaatcatt acgctacatt ggatgagcgt tttaaaatta
    12421 tttctcagtc taatgcagga gtgtctgtcg caagaaataa ggctctatcc cagtgtcaag
    12481 gagagtatat ttgctttgtt gatagtgatg acctgataga acctaatttt ttgataacac
    12541 ttttacagct tataagtcat caggctgatt tggcaatctg tggttttact cgttctatgg
    12601 ttttctgtat ggatgatgct caaggatata tagaaatgag tggtaaacgt tgtatagaaa
    12661 gaattattct agataaaagt ttcaatcctc agatttgttg tatgttattt cgacataata
    12721 ttataaaaga gaaacaatta gaatttgtga tagggtgtac tcgtggtgaa gatcgtgaat
    12781 ttatattgaa atatttagtt tattctaata aggtttgtta tacaaacaat cttctttatc
    12841 actatcgtgt aaatgatcag tcagccatgg caatcttttc tattaaaagt ttgacaagta
    12901 tagaagcttc acaacgcacg gtcaactact ataaaaaagt gaattattct gttagagggc
    12961 ttgaatttga attttcacgt acattgtgga aattcatgat tttagctatt ttgtctgggc
    13021 aaaatgattt atatcaaatc attcgagaaa agtatgatct aaaaagacaa atggaacatc
    13081 tttattctca tccaggacta tccgaaaaac ttacagcgag attgtttgtt tggaatgagg
    13141 cactttttaa atgggtattt tctattatta gatatctata taaaaaaaga tggagatgaa
    13201 taaacgaaat agattatctg aacaagaaat aaaaaaagta gaacttgacg ctttattgta
    13261 tattcggaaa atttgtcagc aatatcatct tcgctattat ttagcttggg gtactttgtt
    13321 gggagccgtt aggcataaag gatttattcc ttgggatgat gatattgata taatgatgcc
    13381 acgttcagat tatgagcgat tattggagtt agttccttcc ttgagacata gttattataa
    13441 aatcttaagt aatccaaaga atgaggatta ttattatact catgcaaaaa tgatagatac
    13501 tcgtactttg tgtgtggaaa atgatttgca aactattcaa aatatgggga tttgggttga
    13561 tattttcccc ttag
//
