LOCUS       c00101_gnlPro..         4875 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HGLMPHCG_101.
ACCESSION   c00101_gnlPro..
VERSION     c00101_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 16:50:06
            Original ID  :: gnl|Prokka|HGLMPHCG_101
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4875
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4875
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1014:4874]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1015..4874
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..4875
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4875
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             2..841
                     /locus_tag="ctg101_1"
                     /transl_table=11
                     /translation="RLLAAIRKTKKWLPSSLVKQCVVYGAGEFLDTSLPASKEKEEEIF
                     GEMLEHLTQEASRTCILIEFRNLDNSMFGYRFFRKNDFFPVNWLRVRNSLHSTQKAEDR
                     FSPSRIRQIRKGLKNGAKVVEAHTVEEIKEFSRMLHKVYSSRIRRYFPANDFFRHMNSM
                     LIRGKQAKIFIVKYKEKIIGGSVCIYSGENAFIWFSGGMRKTYALQSPGILAVWKALED
                     AHERGFRHMEFMDVGLPFRKHGYRDFVLRFGGKQSSTRRWFRISWPWLNNLLVKFYV"
     CDS             1015..1419
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg101_2"
                     /sec_met_domain="Glyoxalase_4 (E-value: 6.4e-25, bitscore:
                     76.4, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKISHIEHLGIAVKSIEEALPYYENVLGLKCYNIETVEDQKVRTA
                     FLKVGETKIELLEPTCPESTIAKFIENKGAGVHHVAFAVEDGVANALAEAESKEIRLID
                     KAPRKGAEGLNIAFLHPKSTLGVLTELCEH"
     CDS             1450..3003
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg101_3"
                     /sec_met_domain="CT_Btheta (E-value: 2e-291, bitscore:
                     955.2, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKIKELIERRAVARIGGGEKAIAKQHEKGKYTARERIAML
                     LDEGSFEEMDMFVEHRCTNFGMEKKHYPGDGVVTGCGTIEGRLVYLFAQDFTVTAGSLS
                     ETMSLKICKIMDQAMKMGAPCIGINDSGGARIQEGINALAGYAEIFQRNILASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMMEGTSYMFLTGPKVVKTVTGEDVSQENLGGASVHST
                     KSGVTHFTAKTEEEGLALIRTLLSYIPQNNLEEAPYVDCTDPIDRLEDSLNDIIPDSPN
                     KPYDMYEVISAIVDNGEFLEIQKDYAKNIIIGFARFNGQSVGIVANQPKFLAGVLDSNA
                     SRKGARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTI
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVEVLYAREAKDQENPAQFLA
                     EKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRVIRALQQLQTKKLSNPAKKHGNIPL
                     "
     CDS             3035..3955
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg101_4"
                     /sec_met_domain="OAD_gamma (E-value: 1.7e-15, bitscore:
                     46.2, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTKIGIFLSLLLLIGLTSCGEQKSNNKLLLNEVLIDNQNNFQD
                     DYGLHSAWIEIFNKSYGSADLAACLLKVSSQPGDTVTYFIPKGDILTLVKPRQHALFWA
                     DGEPNRGTFHTSFKLNPETANWVGLFDSGRKLLDQVVIPAGTLGPNQSYARISDGAADW
                     EVKGGSKDKYVTPSTNNKTLDSNAKMEKFEEHDSVGIGMSISAMSVVFCGLILLFIAFK
                     VVGKVAVNLSKRNAMKAKGIDKVEAKELSQAPGEVYAAISMALHEMQDEVHDVEETVLT
                     ITRVKRSYSPWSSKIYTLRENPNRK"
     CDS             3980..4414
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 66.7; E-value:
                     4e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg101_5"
                     /sec_met_domain="Biotin_lipoyl (E-value: 3.9e-22, bitscore:
                     66.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEYKYKINGNSYKVTIGDIEDNIAHVEVNGTHYKVEMEKQPKAT
                     PKPAVVRPMPNSPAAPTTPVVKPAAPSTGKSGVKSPLPGVILDIKVNVGDTVKRGQTII
                     ILEAMKMENNINADKDGKVTAINVNKGDSVLEGNDLVIIE"
     CDS             4416..4874
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg101_6"
                     /sec_met_domain="OAD_beta (E-value: 1.5e-44, bitscore:
                     141.0, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDFINFLGNNLADFWTYTGFANATGGHIAMIIIGLGFIYLAVAK
                     EFEPMLLIPIGFGILIGNIPFNMDAGLKVGIYEEGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYMIALEMGFDPMQAGAIGIIG"
ORIGIN
        1 acgcctgctt gccgccatac gcaagaccaa aaagtggctt ccctcctctc ttgtcaaaca
       61 atgtgtggta tatggtgcag gtgaatttct ggacacttct ctaccggcaa gtaaagagaa
      121 ggaggaagaa attttcggtg aaatgctgga gcatctcaca caagaagcat cacgcacctg
      181 cattcttatt gaattccgca atctggataa ttccatgttc ggttaccgtt tcttccgaaa
      241 gaacgacttt tttccagtca actggctgcg tgtccgtaac tctctgcata gtacgcagaa
      301 agcggaagac cgtttcagcc cgtcacgtat ccgtcagatc cggaaaggac tcaaaaacgg
      361 agccaaagta gtagaagccc atacggtgga agagattaag gaattctcgc ggatgctgca
      421 caaagtttat tcttcacgaa tacggagata tttccctgcc aatgactttt tccgccacat
      481 gaacagcatg cttatcagag gaaaacaggc aaaaatattc atcgtaaaat acaaggaaaa
      541 gattatcggc ggctccgtct gcatctattc cggcgaaaat gcttttatct ggttttcagg
      601 aggaatgcga aagacctatg cactgcagtc acccggcata ctggcagtat ggaaagcact
      661 tgaagatgcc catgaacggg gattccgtca catggagttc atggatgtag gacttccctt
      721 ccgcaagcat ggttatcgtg atttcgtact ccgtttcgga ggcaaacaaa gcagtacccg
      781 ccgctggttc cgtatcagct ggccatggct gaataatctt ctggtcaagt tttatgtcta
      841 aatgtaatct caagattaca aaacccgtct ttgtcaccca tccggagcat tttttatgct
      901 ttattcccca ataattaaat gcagaaatat ttctttatta gacaagaaat gtctttcttt
      961 gtgctgtttt atgccatata agcacataat taaaccatat aaaaaaatat agttatgaag
     1021 atttcacaca ttgagcatct gggcattgct gtaaaaagca tcgaagaagc ccttccttac
     1081 tacgaaaacg tattgggtct gaaatgttac aacatcgaaa cagtagaaga tcaaaaagta
     1141 agaaccgctt ttttaaaggt aggtgaaaca aaaatcgaat tgttggagcc gacttgcccc
     1201 gaaagcacca ttgctaaatt tattgaaaac aaaggtgcag gtgttcatca cgtagcattt
     1261 gccgtagaag atggcgtagc taatgctttg gcagaagcag aaagcaaaga aatccgtctg
     1321 attgacaagg ctccccgcaa aggtgccgaa ggtctgaaca tcgctttcct tcacccgaaa
     1381 tcaacactgg gtgtgctgac agaactctgc gaacactaat cataaactct aaaaaaacaa
     1441 caagaactca tgagtaatca acttgaaaaa attaaagagc ttattgaacg ccgtgccgtt
     1501 gcacgcatcg gaggcggcga aaaagcaatt gcgaagcaac atgaaaaagg gaaatacaca
     1561 gcgcgtgagc gtatagccat gctgttggat gaaggtagct tcgaagaaat ggacatgttc
     1621 gttgagcaca gatgcacgaa cttcggcatg gagaaaaagc actatccggg tgatggtgta
     1681 gtaaccggtt gcggtactat cgaaggtcgt ctcgtatatc tttttgcaca ggactttacc
     1741 gttactgcag gttcactgtc ggaaacaatg tcactgaaga tctgtaagat tatggatcag
     1801 gcaatgaaga tgggtgctcc ttgtatcggt atcaatgact cgggcggtgc acgtatccag
     1861 gaaggtatta acgctttggc aggttacgca gaaatcttcc agcgcaatat cctcgcttcg
     1921 ggtgtcattc cgcagatttc cggtatcttc ggtccttgtg ccggcggtgc tgtttattct
     1981 ccggcactga cagacttcac actgatgatg gaaggtactt cttatatgtt ccttacaggt
     2041 ccgaaagttg tgaaaactgt aaccggtgaa gacgtaagcc aggaaaacct cggcggagca
     2101 agcgttcact ctaccaaatc gggtgttact catttcactg ccaaaacaga agaagaaggt
     2161 ctggcattga tccgcacact tctgagctat attcctcaaa acaacctcga agaagctcca
     2221 tacgtggatt gcacagatcc gatcgaccgt ctggaagatt cactgaacga tattatcccc
     2281 gacagcccca acaaaccgta tgatatgtac gaggttatca gcgctatcgt agacaacggc
     2341 gaattcctgg aaatccagaa agactatgcc aagaatatca ttatcggttt tgcccgtttc
     2401 aacggtcagt ctgtaggtat tgtagccaat cagcctaaat tccttgccgg tgtactcgac
     2461 agcaacgcat ctcgcaaagg cgcacgtttc gttcgtttct gcgatgcttt caatatccct
     2521 atcgtgtcgt tggtagacgt accgggattc cttccgggaa caggccagga atacaatggc
     2581 gttatccttc atggtgccaa actgctgtat gcttacggtg aagctactgt acctaaagta
     2641 acaatcacat tgcgtaaatc ttacggtggt tctcacatcg tgatgagttg taaacaactt
     2701 cgtggtgaca tgaactatgc ttggccaaca gctgaaatcg ctgtaatggg tggtgcaggt
     2761 gcagtagaag tattgtacgc gcgtgaagcc aaagaccagg aaaatccggc tcagttcctt
     2821 gccgaaaaag aagcagaata tacaaaactg tttgccaacc cttataatgc agctaagtat
     2881 ggttacatcg atgatgtcat cgaaccccgt aacacacgtt tccgcgtgat ccgtgctttg
     2941 cagcagttgc agactaagaa actgtctaat ccggcgaaga aacatggtaa tattccgttg
     3001 taatccaact tttcacatta aaacaagaac gattatgaac aaaaccaaaa tcggaatatt
     3061 cctttctctg ctgttgctga ttggattgac ttcctgcgga gagcaaaagt cgaacaataa
     3121 gctattgctg aatgaagtcc tgatagacaa tcaaaacaac tttcaggatg actacggctt
     3181 gcacagtgca tggattgaaa tattcaacaa atcatacggt agcgcagact tagcagcttg
     3241 tttattgaaa gtaagcagcc agccgggcga tacagtaacc tattttatcc caaaaggtga
     3301 catacttaca ttagtaaagc cacgccaaca tgcgttattt tgggcagatg gcgaacctaa
     3361 tcgtggaacc ttccatacca gcttcaaact gaatcccgaa acagccaatt gggtcggtct
     3421 ttttgattca ggcagaaagc tacttgatca agttgtcata ccagcaggaa ctttaggtcc
     3481 caaccagtct tatgcacgca taagcgacgg agcagcagac tgggaagtaa aaggcggaag
     3541 caaagacaaa tacgtaaccc cgagcacgaa caacaaaacc cttgacagta atgccaagat
     3601 ggaaaagttc gaagaacatg actcagtcgg tatcggtatg tctatttcag ccatgagcgt
     3661 agtattctgc ggattgattc ttctcttcat tgccttcaag gtggtaggta aagttgctgt
     3721 caacctgagc aaacgcaatg cgatgaaggc taaaggcatc gataaagtcg aagcgaaaga
     3781 gctttcacag gctccgggtg aagtttatgc tgctatttca atggcattac acgaaatgca
     3841 ggatgaggta cacgacgtag aagaaacggt gctgaccatc actcgtgtga agcgcagtta
     3901 ttcaccgtgg agttcgaaga tttacacctt gcgtgaaaat cctaacagaa agtaaatcat
     3961 tccctaaaaa gataaaacga tgaaagaata taaatataaa atcaacggta actcatacaa
     4021 agtaaccatc ggagatattg aagataatat cgctcatgta gaagtgaacg gtacgcatta
     4081 caaagtagaa atggagaaac aaccgaaagc tactcccaaa ccggcagtag tacgccctat
     4141 gcctaactca ccggctgctc ctaccacacc ggtagtaaaa ccggctgctc cttctaccgg
     4201 caaatcaggc gttaagtccc cactgccagg cgttatcctt gacatcaaag taaatgtagg
     4261 tgatacagtg aaaagaggac agactatcat tatattggaa gccatgaaga tggaaaacaa
     4321 tatcaatgca gacaaagacg gtaaagtgac tgccatcaat gtcaacaagg gagattctgt
     4381 tcttgaaggt aatgacctcg taattattga ataatatggg agattttatt aactttttag
     4441 gaaataacct tgccgatttc tggacctaca cgggatttgc caacgcgaca ggtggacata
     4501 ttgctatgat cattatcggt ttaggcttta tttacttagc tgtagccaaa gagttcgagc
     4561 cgatgttgtt aattcctatc ggattcggta ttctgatcgg taatatacct ttcaatatgg
     4621 acgccggact aaaagtcggt atatatgaag aaggttctgt attgaacata ttgtatcagg
     4681 gagtaacgtc cggctggtat ccaccgctca tcttcttagg tatcggtgcc atgacagact
     4741 tctcggcgct aatctccaat ccgaagctga tgctgatcgg tgcggctgcc cagtttggta
     4801 tcttcggtgc atacatgatc gcattggaaa tgggatttga cccgatgcag gccggtgcta
     4861 tcggtatcat cggtg
//
