LOCUS       c00231_gnlPro..         3447 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HGLMPHCG_231.
ACCESSION   c00231_gnlPro..
VERSION     c00231_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 16:50:06
            Original ID  :: gnl|Prokka|HGLMPHCG_231
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3447
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..396
                     /locus_tag="ctg231_1"
                     /transl_table=11
                     /translation="IQFRIIDGYILAFPGFSCWGYHYYIRKSGEIEPMRDEDTVGAHAR
                     GYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELLQRYPEAKVVGHRDLSPDT
                     NYNGIVDPWERIKECPCFEVIGEFSIN"
     protocluster    1..3447
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[388:3359]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      389..3359
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3447
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3447
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(389..1117)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 71.7;
                     E-value: 2.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg231_2"
                     /sec_met_domain="Radical_SAM (E-value: 6.7e-30, bitscore:
                     92.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKTLVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(1131..3359)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg231_3"
                     /sec_met_domain="Gly_radical (E-value: 1.9e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     KFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 attcagttcc gaatcataga cggttacatt ctcgcattcc ctggcttttc gtgctggggg
       61 tatcattatt atatacgcaa gtcgggcgag attgagccga tgcgggatga agatacggtc
      121 ggagctcatg cacgtggcta taatgcgatc agcttaggtg tatgttatga gggcggtctg
      181 gatgagaatg gaaaagctgc ggatacgcgt acaccccgtc agaaggaggc gctacatcgc
      241 ttggtacacg aattgttgca gcgttatccg gaagctaaag tagtagggca tcgggattta
      301 agtcccgaca ccaactataa tgggatcgta gatccttggg aacggattaa agaatgccct
      361 tgctttgagg tcattggaga attctccatt aattaactac caccgtagtg aaatactctt
      421 tgaatacctc cgcagctttt tcaagctgtt cgggggtatt ttcttttacc cctttcatct
      481 tgtattcctg tcccatcgct tcgtacttat gtacgcccag tgtatggtaa ggcagtattt
      541 ccacccgttg gatcatcttg tattttccga gtgcttctcc cagtgcgcgt atgtcttctt
      601 caaaatcgct gtatccgggc actagcacgt agcgtagcca gaaaggtttc tcctgttctt
      661 ccagccaggc ggccgtgcgg atggtttgct cgttgcttct tccggtgagc gtctgatggc
      721 ggttggggtt gaattccttt atatccaata acacaagatc ggtcagtctg aataattctt
      781 ctacgtcttc gttccaaagt cctccgttgc tgtccagaca gacatggatg cctttttctt
      841 tcagttcgcg gaccagcggg acgagtgttt tcgcctgaaa cgtcggttcg cctccggaga
      901 aagtgattcc tcctcgtttc ccgaagaagg ggcgctggct cattgccatg cggacgattt
      961 cttccggtgg ggtaggcgtg cctccctttc ctgctatcgt atccggattg gcgcaataga
     1021 ggcagcggaa gttgcatcct tgaagaaaaa cgacgagccg taagcccggc ccgtcgaatg
     1081 ttcccatact ttcgtatgaa tgtacgttta tcatcatata gagtaataga ttacatacgt
     1141 tcgtggaagc tacggctgat aacttccagt tggtgttcgc ggctcagctt cacgaagttt
     1201 acggcatagc cggaaacgcg gatagtcagc tgcggatagt tttccggatg ctccatggca
     1261 tcgtacagca tatcacggtt cagtacgttt acgttcaggt ggtgagcacc tttggtgaag
     1321 taaccgtcca tcatcgttac gaggttctcg atacggtctt cgtccgtagc acccagtgat
     1381 ttcggaacga tagagaaggt gttgctgata ccgtcctgtg agtcacggta acgaagtttc
     1441 gctacagagc tcagagaagc gatcgcacca ttcttgtcgc gtccgtgcat cgggttggca
     1501 cccggagcga aggcaacacc tttggcacgt ccgtcgggag tagcaccggt ctttttgccg
     1561 tacatcacat tggaagtgat agtcagcaag gagagggtag gacgggcatt cttgtaaact
     1621 ggcaatttct tcaattcttc gctgaagaag tataccaggt ctacgccaag gtggtctacc
     1681 ttgtcgttgt cgttaccgaa gcaagggaat tctccttcga tgtcgaaacc ttctgtcagg
     1741 ccgatatcgt tgcggcgggc agttactttc gcatatttga tggcagacag tgaatcgagg
     1801 gcgatggaaa gtccggctac accgtaagcc aggttgatgc gcgggttcgt atctacaaga
     1861 gccatctggg ccttctcgta gtaatacttg tcgtgcatat agtggatgat gttcatcgct
     1921 tcgttatata cgcgggcgat ttcggtcagc acttttttgt agttgtccat tacttcttcg
     1981 aacttcagcg tgtcactggt cagtacgggg atgttcttca ccatgactgt accggtattc
     2041 tcgcaacgtc cgccgttgat ggcaagcaac agggctttgg ccaggttgca acgggcaccg
     2101 aagaactgaa tctgcttgcc tatttcctgg tatgatacgc agcaggcgat tccgtagtcg
     2161 tcggactgac gtacttcacg catcaggtcg tcgttttcgt actggataga agaagtgtcg
     2221 atagaaacct ttgcacagaa ttctttgaat ccttccggaa gttccgggct ccagagtacg
     2281 gtcaggttcg gttccggtga agggccgagg ttgtacaatg tctgcagaaa acggaaggag
     2341 gtctttgtca ctttggtacg tccgtcgttg agacgtccgc ccagagattc ggttacccag
     2401 gtcgggtcgc ctgcaaagat gtcgttgtat gattgcatac gcaggtggcg gaccatacgc
     2461 agtttgatga caaactggtc gatcagctct tgcgcgaacg attcggtgat ggttcctttg
     2521 ctcagttcat attccagata gatatcgagg aaagaagaaa cgttacccag tgacatagcg
     2581 gcaccgtctt gttctttgac ggcggcaagg taagccatat atacccactg tacggcttct
     2641 tgtgccgtgt aagcgggacg gctcaggtcg agaccgtagt attcgcccat cactttcatg
     2701 tctttcagtg ccttgatctg ttctgccact tcttcgcgca ggcggatgcg ggcctctgtc
     2761 atcggaccgg tgaggttgtg caaatcttcc ttctttgctt cgatcagacg gtcgatgccg
     2821 taaagagcca tacggcggta gtcaccgatg atgcgtccgc gtgcataatt gtcgggaagt
     2881 ccggtaagga atcccagcga acggaaggaa cggatttctt ccgtatatac gtcgaatact
     2941 ccgtcgttgt gcgtcttgcg gtagtgagtg aaaatatctt ttacgcggtc gtccacttcc
     3001 acgccgtttt cgtgacaggc tttgctgact acgttgatac ctccgaaagg cttgatggca
     3061 cgtttcagaa gttcgtctgt ctgcaggccg acgatcagtt cgttttcctt gtcgatatat
     3121 ccggccttgt gggaagtgat ggttgatacg gtaacattat ctaatgagcg gacaccgttg
     3181 ttttctcttt cttctgccag tgcttcgagg cagcggttcc atacggcttt ggtacgttct
     3241 gtaggtcctt cgaggaatga agcatctccg taatacggag tgatgttatg acttacgaaa
     3301 tctctgacgt tgatttcgct gctccaaaga ccgtctttaa agatcttgtt taattccata
     3361 aaatgataaa attagaggtt gtagttatcc tattctttag agcgcacaaa gttacaatca
     3421 tttttcatat aatcagcata aatatgc
//
