LOCUS       c00146_gnlPro..        16282 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OAFNIPAF_146.
ACCESSION   c00146_gnlPro..
VERSION     c00146_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 17:26:16
            Original ID  :: gnl|Prokka|OAFNIPAF_146
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 4930
            Orig. end    :: 21212
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..16282
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[14930:19492]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      10001..14562
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..16282
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..16282
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(113..634)
                     /locus_tag="ctg146_6"
                     /transl_table=11
                     /translation="MSTINYDLSRIKALAFDVDGVLSSTTVPLHPSGEPMRTVNIKDGY
                     AIQLAVKKGLHIAIITGGRTEAVRIRFAALGVKDLYMGSAVKIHDYRDFRDKYGLSDDE
                     ILYMGDDVPDIEVMRECGLPCCPKDAVPEVKSVAKYISYADGGRGCGRDVVEQVLKAHG
                     KWMAEDAFGW"
     CDS             complement(615..1409)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Shikimate_DH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) F420_oxidored"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg146_7"
                     /sec_met_domain="Shikimate_DH (E-value: 8.9e-11, bitscore:
                     32.5, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="F420_oxidored (E-value: 4.9e-13, bitscore:
                     40.1, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRSIEDTPVVFLGAGNLATNLAKALYRKGFRIMQVYSRTEESAR
                     TLANEVEAEYITDLKGVSNEARLYIISLKDAAFVELLPQITDGKQHALLVHTAGSIPMN
                     IWEGHAERYGVFYPMQTFSKQREVDFREVPFFIEAKRPEDAELLKAVAGTLSDKVYEAD
                     SEQRRSLHLAAVFTCNFTNHMYALAAELLEKYHLPFDVMLPLIDETARKVHELAPRDAQ
                     TGPAVRYDENVMNKHLSMLADSQALQEIYKLMSKSIHEHHQL"
     CDS             complement(1417..1752)
                     /locus_tag="ctg146_8"
                     /transl_table=11
                     /translation="MGAVKANNNKDTYKATAVAFIVIGTLFLINKLLPFASIGLPWVMN
                     KDNLLLYASISFLIFKRDKSVGFVLLGLWLVMNIGLVMSLLGSLSGYLLPLALLIIGII
                     LFWVSKR"
     CDS             complement(1755..2468)
                     /locus_tag="ctg146_9"
                     /transl_table=11
                     /translation="VLFDFCLTAGFTIRFDVIVLCNSFFHNEYISQCKDTISFLITIGL
                     RYLCIHKSNENGNNMENFSELIKNRRSMRKFTDEELTQDEVVALMKAALMSPSSKRSNS
                     WQFVVVDDKEKLKELSHCKEQASSFIADAALAIVVMADPLASDVWIEDASIASIMIQLQ
                     AEDLGLGSCWVQVRERFTATGMPSDEFVHGILDIPLQLQILSVIAIGHKGMERKPFNEE
                     HLQWEKIHINKFGGK"
     CDS             complement(2690..3232)
                     /locus_tag="ctg146_10"
                     /transl_table=11
                     /translation="MIEEMLAYNREFVKNEGYKEYITNKYPDKKIAILSCMDTRLTALL
                     PAALGIKNGDVKMIKNAGGVISHPFGSVIRSLLVAIFELGVEEIMVIAHSDCGACHMHS
                     EEMLEKMKARGINADYIHMMSFCGVDFHSWLDGFEDTEKSVRGTVDFIVHHPLIPSDVK
                     VHGFIIDSTTGELTRIV"
     CDS             complement(3419..5026)
                     /locus_tag="ctg146_11"
                     /transl_table=11
                     /translation="VIDGAENVTGDENEINECVAQARCLRAYYYMNLVEQFGNVDLQLK
                     AADSENISFDAHRSTVPEIYAAIIEDLKFAVENLPVSFSDYYSRVTKKSAMGLLARAYI
                     NGAGYDLKDIDGVSFLEKAYDTATTMINNKAIYEWYMHPAFADVFNENNNRNNEEALFI
                     AAGAERNSDAYTNGNYSQSEMFRHFLPSLGTYTDLGLVDKTSNFVYGRPNSNIFLPSKY
                     LMDCFAADMNDSRFRYSFISAYSSYSIPAWGATYEYGGSACAKEITSTLATKFGIPASN
                     IGKKVYPHFNLESNSTADANYCQLAIWNADGTAKTTQDKTDGNILHPAMPLDPAEAHQY
                     AVYCSLKTLTEEEKAQYPGLVLNVFDLYDENGTARATYDKPSAASALWLSIYPALSKFN
                     MPGNKFVGRDVQRKTVDVMIMRMAEVYLIAAEASVRLNKGDAAKYINVLRTRAGAGTVS
                     ESQVDLNYIFDEYARELCGECGRWYLLKRNAAFETRLAAYNKRAAEHFKKEFYLRPIPT
                     KYLDAINNPAEFGQNAGY"
     CDS             5111..5350
                     /locus_tag="ctg146_12"
                     /transl_table=11
                     /translation="MITLIAGARFRFFPNISTRFRTNQIVTTLIDVVDQGCIARVGECL
                     ISFQCIRTIGELTRVVGVHFIKTARAPRKNTGNK"
     CDS             complement(5386..8526)
                     /locus_tag="ctg146_13"
                     /transl_table=11
                     /translation="MILGLTLLCAPSAISVYAENGVNDITVQAVMQTKTVKGTVLDENG
                     EPLIGVSIVVKGTSTGTITDFDGKFSINLPGGSKELVVSYIGYKEQAIIVSGNAPLNIK
                     MVPDTQALDEVVVIGYGTVKKRDLTGAVASVKSEDITMNPGVNPMDALQGKIAGLDITN
                     SSGQAGSSPTVQLRGTRSLQLDEDGNISSDAFKPTYIIDGMPGDIATLNPNDIESIEVL
                     KDASSTAIYGSSGANGVIIVTTKGSKSGKPVINFNAYAGTTMGARVPKMRTGDSYINYI
                     KDSYKPVGTTNEEEIFGERYDAIKNNQWVNWGDEVLHSGLKQNYSISVAGGTEKTKAYF
                     SLNYTDEKSMYENDDYKVYSTRVRIDQEINKWMNAGINVQASFSNKNSRNAVLERALFA
                     TPLGVPYNEDGSITEFPIPGSSSDPNPLADEQDGVYKNNTKAGRAYVDAYLEWKPVKGL
                     SVKSQLGGSYSQSRAGKFMGEGSYNVLKGSTVAYGEATNKTGYNYKWENIVTYHNTFNK
                     DHDLTVTGVTSWNYNQSEEYYVYGENPATNDMLWYALQNADNKKLNSKYLMSKGMGFIG
                     RVNYSYKGKYLFSASSRYEADSRLSKDNRWNLFPAVSVGWRISDEAFMAGTKSWLDNLK
                     LRVGYGVAGTTAGIAEYSSMASLENVTTSLGGMTVPSAKFTEYITNRNLTWEKTHDLNI
                     GIDASFLNNRIDLTVDLYRTKTTDVILSQALPTSIMGNYTGSTTYKMNKNAAETENRGI
                     ELALNTRNIVKKDFTWSSTVTFTANKEKIKSLIEGQDMMFNAKKGDMVFKVGEGVGSFY
                     RYKVLGIWQYSEKETAALFGCEPGDIKLDLPSVKKDGNGYYYMNPEGERVDITAENPYG
                     VRADYDRQVIGRNTPDWTLGFKNNFRYKDFDLSVFLYARWGQMMNYGSVIGKYSPNPDY
                     NIPTYFTYYDKTIEADQNVLFYAIDKSKDRSAYEGYDSMYYVDGSFFKLKNVTLGYTLP
                     GKLTKRFGISNLRVYATMTNLFTYSPNKYVKNYDPEMNGSINFPLSRDCIFGLNLTF"
     CDS             9051..9827
                     /locus_tag="ctg146_14"
                     /transl_table=11
                     /translation="VVYGAGEFLDTSLPASKEKEEEIFGEMLEHLTQEASRTCILIEFR
                     NLDNSMFGYRFFRKNDFFPVNWLRVRNSLHSTQKAEDRFSPSRIRQIRKGLKNGAKVVE
                     AHTVEEIKEFSRMLHKVYSSRIRRYFPANDFFRHMNSMLIRGKQAKIFIVKYKEKIIGG
                     SVCIYSGENAFIWFSGGMRKTYALQSPGILAVWKALEDAHERGFRHMEFMDVGLPFRKH
                     GYRDFVLRFGGKQSSTRRWFRISWPWLNNLLVKFYV"
     CDS             10001..10405
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg146_15"
                     /sec_met_domain="Glyoxalase_4 (E-value: 2.2e-24, bitscore:
                     76.4, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKISHIEHLGIAVKSIEEALPYYENVLGLKCYNIETVEDQKVRTA
                     FLKVGETKIELLEPTCPESTIAKFIENKGAGVHHVAFAVEDGVANALAEAESKEIRLID
                     KAPRKGAEGLNIAFLHPKSTLGVLTELCEH"
     CDS             10436..11989
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg146_16"
                     /sec_met_domain="CT_Btheta (E-value: 1.7e-290, bitscore:
                     953.9, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKIKELIERRAVARIGGGEKAIAKQHEKGKYTARERLAML
                     LDEGSFEEMDMFVEHRCTNFGMEKKHYPGDGVVTGCGTIEGRLVYLFAQDFTVTAGSLS
                     ETMSLKICKIMDQAMKMGAPCIGINDSGGARIQEGINALAGYAEIFQRNILASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMMEGTSYMFLTGPKVVKTVTGEDVSQENLGGASVHST
                     KSGVTHFTAKTEEEGLALIRTLLSYIPQNNLEEAPYVDCTDPIDRLEDSLNDIIPDSPN
                     KPYDMYEVISAIVDNGEFLEIQKDYAKNIIIGFARFNGQSVGIVANQPKFLAGVLDSNA
                     SRKGARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTI
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVEVLYAREAKDQENPAQFLA
                     EKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRVIRALQQLQTKKLSNPAKKHGNIPL
                     "
     CDS             12021..12941
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg146_17"
                     /sec_met_domain="OAD_gamma (E-value: 5.8e-15, bitscore:
                     46.2, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTKIGIFLSLLLLIGLTSCGEQKSNNKLLLNEVLIDNQNNFQD
                     DYGLHSAWIEIFNKSYGSADLAACLLKVSSQPGDTVTYFIPKGDILTLVKPRQHALFWA
                     DGEPNRGTFHTSFKLNPETANWVGLFDSGRKLLDQVVIPAGTLGPNQSYARISDGAADW
                     EVKGGSKDKYVTPSTNNKTLDSNAKMEKFEEHDSVGIGMSISAMSVVFCGLILLFIAFK
                     VVGKVAVNLSKRNAMKAKGIDKVEAKELSQAPGEVYAAISMALHEMQDEVHDVEETVLT
                     ITRVKRSYSPWSSKIYTLRENPNRK"
     CDS             12966..13400
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 66.7; E-value:
                     4e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg146_18"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1.4e-21, bitscore:
                     66.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEYKYKINGNSYKVTIGDIEDNIAHVEVNGTHYKVEMEKQPKAT
                     PKPAVVRPMPNSPAAPTTPVVKPAAPSTGKSGVKSPLPGVILDIKVNVGDTVKRGQTII
                     ILEAMKMENNINADKDGKVTAINVNKGDSVLEGNDLVIIE"
     CDS             13402..14562
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg146_19"
                     /sec_met_domain="OAD_beta (E-value: 3.1e-154, bitscore:
                     503.5, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDFINFLGNNLADFWTYTGFANATVGHIVMILVGLFFIYLAVAK
                     EFEPMLLIPIGFGILIGNIPFNMDAGLKVGIYEEGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYMIALEMGFDPMQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPVIQPPIMRLLTTKHERVIRMKPPRAVSHTEKVIFP
                     IIGLLLTCFLVPSGLPLLGMLFFGNLLKESGVTRRLANTASGPLIDVITILLGLTVGAS
                     TQASEFLTLDSIKIFALGALSFIIATASGVIFVKIFNLFLKKGNKINPLIGNAGVSAVP
                     DSARISQVVGLEYDPTNYLLMHAMGPNVAGVIGSAVAAGILLGFLM"
     CDS             14776..16110
                     /locus_tag="ctg146_20"
                     /transl_table=11
                     /translation="MKRIILLAFLCTTTLVMAQRINHVQEAIANYDYEAALTLIAKEKP
                     TIPLLLQKGKAQRGLGMNTEALSTYQEIITNDTANTRAYIEAAECCRSLAKYQQALKYY
                     EQALDLNPENKYVRIQYIGLLLSLQKFQDALGESSLMTEKDSSAIALHLQAQSFEGMGE
                     LLPATGCYYNIQEKYPDDYLAAAKLAALNIAGSYFNEAIEATEKYRQIDTTNIAVNRQN
                     ALAYCLNKDYPTAIQRYEYLVSQGDSSFHTCYYLGISYYAEEKYYEAHDFLEAARKHDP
                     ENVNLLYYLGRACAKTSWKKLGVEYLEKALDLTIPKDSNMVRLYIGMTDCYKMAQMPKE
                     QIESIRERYRKYDKQNHKLLYDMAFIYFYSLKDKKNTERCLEAFLKTRPKEDKEEEAKL
                     NERGELVLGTKNYYNAAANWLKDIQSKQKIEDFFLGGNVPAQKPE"
     CDS             complement(16189..16281)
                     /locus_tag="ctg146_21"
                     /transl_table=11
                     /translation="KYLGPARDNMEKLYKHKILNVLGSDNKLAE"
ORIGIN
        1 ctcccaagcc tgacatcagt tctttccggc ggggagaatt ggaggctaat ataatctgat
       61 atttatctaa atttccaagc atagttttat ttatcattta gtgatttaca actcaccagc
      121 cgaaagcatc ttccgccatc cacttgccat gagctttcaa tacttgttcc accacatcac
      181 gtccacagcc acgaccgcca tcggcataag atatatactt cgcaaccgac ttaacttccg
      241 gaactgcatc tttgggacaa caaggcaacc cgcactcacg catcacttct atatcaggca
      301 catcatcgcc catatacaga atttcgtcat cagacagacc gtatttatcg cggaaatcac
      361 ggtaatcatg aattttcacc gcagacccca tatacagatc cttcacaccc aatgctgcaa
      421 accggatacg taccgcttct gtccgtccac cggtaatgat agcaatatga agtcctttct
      481 ttaccgccag ctgaatggca taaccgtctt tgatattcac tgtacgcatc ggttctccgg
      541 aaggatgcaa cgggactgtt gtcgaactca atactccgtc aacatcaaaa gccagtgctt
      601 tgatgcggga tagatcataa ttgatggtgc tcatggatac ttttactcat taatttataa
      661 atttcctgca atgcctgact gtctgccagc atagaaagat gcttattcat cacattctcg
      721 tcatagcgta cagccggtcc ggtctgcgca tcacgaggag cgagttcatg cactttgcgt
      781 gccgtttcgt caatcagggg aagcatgaca tcaaatggca aatgatattt ttccagcaat
      841 tcggcggcaa gagcatacat atggttggta aagttacagg taaagacagc tgccagatga
      901 agacttctgc gttgctccga atcggcttcg tacaccttat cagagagcgt accggcaacc
      961 gctttcagca gctcggcatc ttccggtctt tttgcctcaa taaagaaagg tacttcccga
     1021 aaatcaactt cacgttgttt gctgaatgtc tgcatcggat aaaatactcc gtagcgttcc
     1081 gcatgacctt cccaaatatt catcgggatg ctacctgccg tatgtaccag cagcgcatgc
     1141 tgcttcccat cagtgatctg aggcaacagt tcgacgaaag cggcatcttt caatgaaata
     1201 atatataatc tcgcttcatt cgatacgcct tttaaatcag tgatatattc cgcttccact
     1261 tcattggcta acgtacgagc cgattcttcc gttcggctgt acacctgcat gatacggaat
     1321 cctttccggt aaagcgcttt cgccagatta gttgccagat ttccggcacc gagaaacaca
     1381 acgggcgtat cttcaatact tcttttcatt gtttctttaa cgttttgaaa cccaaaataa
     1441 aataattccg atgatcaaca gtgccaatgg caacagataa ccggacaagg aaccgagcaa
     1501 tgacattacc agaccgatat tcattaccag ccacagtccc aacagcacaa agccgaccga
     1561 cttgtcacgc ttgaaaataa gaaaactgat agaggcatac aacaacaaat tgtccttatt
     1621 catcacccaa ggcaaaccta tagaagcaaa ggggagcagt ttatttatca ggaaaagagt
     1681 tccgatgaca ataaaagcga cggcagtagc cttataagta tctttattat tatttgcttt
     1741 gacagctccc atcgttattt tcctccgaac ttattaatat gaattttctc ccactggagg
     1801 tgttcttcgt tgaaaggctt acgttccatt cccttatgtc cgatggcaat cacggaaaga
     1861 atctgcagtt gcaaaggaat atccaaaatt ccatgaacaa actcgtcaga cggcattccg
     1921 gttgcagtga aacgttcgcg aacctgtacc caacagcttc ccagccccaa atcttccgcc
     1981 tgcaactgta tcatgatgga agcaatagaa gcatcttcaa tccagacatc gctcgccaat
     2041 ggatctgcca tcacgacaat cgccaaagca gcgtctgcaa tgaaagaaga agcctgttct
     2101 ttgcagtgag acagttcttt caacttttct ttatcatcga ctaccacaaa ctgccagcta
     2161 ttactacgtt tggaactggg agacattaag gcagctttca tcagtgccac aacctcatcc
     2221 tgtgttaact cctcatccgt aaatttgcgc atactgcggc gattctttat cagttcactg
     2281 aaattttcca tattatttcc gttttcattt gatttatgta tgcaaagata gcgcaaaccg
     2341 atcgtaataa gaaatgaaat tgtatctttg cactgagaaa tatattcatt atgaaaaaac
     2401 gaattacaca agacgattac atcaaagcga atcgtaaagc cagccgtgag gcagaaatca
     2461 aagagcacgg acaccccgtt tgttacaaac gagtgcatga gtcaaagaaa gtatataacc
     2521 gtagaaagat aaaggcagcc gataaaaagc tgccttattt ttttcatttg ggatagtgtc
     2581 ggtatcccaa tgacatagtg acaccatgag acagtgatag tgacactatc ctgttgcgat
     2641 agtgacacca tgttttatgc agttcctgat aggcagaaag ggcgatttcc tatacaattc
     2701 gcgtcagttc tccagtagtg gagtctatga taaacccgtg cactttcacg tccgacggaa
     2761 tcaaaggatg atgaactata aaatcgaccg ttccccttac cgacttctcc gtatcttcaa
     2821 agccatctag ccaagaatga aaatcgactc cacaaaaact catcatatga atataatcgg
     2881 cattaatacc ccgtgccttc atcttctcaa gcatttcttc actgtgcata tggcaggctc
     2941 cacaatcaga atgggcaatc accattattt cctccacacc taactcgaag atagcgacaa
     3001 gcagactgcg aatcacacta ccgaaaggat gggaaatgac gcctcctgca ttcttgatca
     3061 ttttaacgtc tccgttctta atgcctagtg cggccgggag taaagctgtc aggcgtgtat
     3121 ccatacagga taagatagca atctttttat cgggatactt attcgttata tattctttat
     3181 atccctcgtt cttaacgaac tctctattat aagcaagcat ttcttctatc atagcgctat
     3241 tttgtttgga cacacaaaaa taaagaaaat aataaaaggc tgtatcagga aataatgaaa
     3301 atataccgga aatccttttt aaacttttac gcaggataca ggtatggaaa caaagagccg
     3361 gacaagagaa atctcctgcc cggctcctat taattatgct tgattattaa ttaactgatt
     3421 aataaccggc gttttgtccg aattctgccg gattattgat cgcatccaga tatttcgtag
     3481 gaataggacg caaatagaat tctttcttaa agtgctcggc agcacgctta ttataagcag
     3541 ccaaacgagt ctcaaaagca gcattacgtt tcagcaaata ccagcggcca cattcgccac
     3601 aaagttcacg ggcatattca tcgaaaatat aattcagatc cacttgcgac tcactgaccg
     3661 taccggcacc ggcacgcgta cggagtacat taatatattt ggcagcatct cccttgttca
     3721 agcgtaccga tgcttcagca gcaatcaggt aaacttccgc catacgcatg atcatcacat
     3781 ccacagtttt tctctgaacg tctctgccga caaacttatt acccggcata ttgaatttag
     3841 aaagagccgg ataaatagat aaccacaaag cactggcagc actaggcttg tcataggtag
     3901 ccctggctgt tccattctca tcgtaaagat cgaaaacatt aagaaccaac cccggatatt
     3961 gagctttttc ctcttctgtc agcgtcttca aagaacaata aacggcatat tgatgagctt
     4021 ctgccggatc taaaggcata gcaggatgaa gaatattacc atctgtttta tcctgagtag
     4081 tcttcgctgt tccatctgca ttccagatag ccagctggca gtaattagca tcggctgtac
     4141 tattggattc caagttaaaa tgaggataaa ctttcttacc tatattagat gcgggaatac
     4201 caaatttagt agcaagtgtg cttgtgattt ctttggcaca cgctgaacct ccatattcat
     4261 aagtcgcccc ccatgccggt attgaataag acgagtaagc tgagataaat gaataacgaa
     4321 aacgagagtc gttcatatca gcagcaaaac agtccatcag atatttggat ggcaggaaga
     4381 tattactgtt cggacgaccg taaacgaagt ttgaagtctt gtctaccaaa cccaggtccg
     4441 tatatgttcc caacgaaggt aagaagtgac ggaacatttc actttgtgag tagttaccat
     4501 tggtgtaagc gtccgaattt ctttcggcac ccgcagcaat gaacagagct tcttcattgt
     4561 tacgattatt attttcgttg aatacatcgg caaaagcagg atgcatatac cactcataaa
     4621 tcgctttatt attaatcatg gttgtcgccg tatcataggc tttttccaga aagctgacac
     4681 catcgatatc tttcagatca taaccggcac cattaatata tgcacgtgcc aataatccca
     4741 tcgccgattt ctttgtaacg cggctatagt aatcactgaa acttacgggc agattctcta
     4801 cggcaaactt caggtcttcg ataatggcag cataaatttc gggaacggta gaacggtgag
     4861 catcaaaaga aatattctcg ctatcggcag ccttcaactg caaatcgaca tttccaaact
     4921 gctccaccag attcatataa tagtaagcac gcagacatct ggcctgtgcc acacattcat
     4981 tgatttcatt ctcatcccct gtcacattct cagctccgtc aatcacaata ttacagctgt
     5041 tgatacacag ataagaatat tgccacactt tataataata gctatcatcg gttttcatac
     5101 cgttataact atgataacgc tgatagctgg agcccgattt aggttcttcc caaatatcag
     5161 tacccgcttc cgtacaaacc agatagtcac tactctgata gatgttgttg atcaaggctg
     5221 tatagcacga gttggtgaat gccttataag tttccaatgt ataagaacca tcggagaact
     5281 gacccgagtt gttggggttc acttcatcaa gactgcaaga gccccaagaa aaaacaccgg
     5341 caacaagtag aactaaacta tatatctttt tcatattgct attttttaga aagttaaatt
     5401 aagaccaaat atgcaatcac gagacaatgg gaagttgatg cttccgttca tttccggatc
     5461 gtaattcttc acatatttgt ttggcgaata agtaaacagg ttggtcattg tcgcataaac
     5521 tctcagatta ctgataccaa aacgttttgt aagtttaccg ggtaacgtat aacccaatgt
     5581 tacgttttta agtttaaaga atgatccatc cacataatac atcgaatcat atccttcata
     5641 agcacttcta tccttggatt tatcgatagc atagaacaag acattctgat ctgcttcgat
     5701 cgtcttgtca tagtaagtaa aataagtagg aatattgtaa tccgggttag gcgaatattt
     5761 acctatcaca ctaccatagt tcatcatttg cccccaacgt gcataaagga agacagacaa
     5821 atcaaaatct ttgtatctga aattattctt aaagcccaat gtccagtccg gagtattacg
     5881 acctattacc tgacgatcat aatccgcacg tacaccataa ggattttctg ccgtaatatc
     5941 aacgcgctct ccttcaggat tcatataata gtaaccattt ccgtccttct tcacactagg
     6001 taaatccagc ttaatatcgc ccggttcgca tccgaacaaa gcagcagttt ccttttcgct
     6061 atattgccag atacccaaca ctttatatct atagaaagaa ccgactcctt cgccgacttt
     6121 gaacaccata tcaccctttt ttgcattgaa catcatatcc tgcccttcga tcaaagattt
     6181 aattttttct ttgttggcgg taaatgtcac tgttgagctc caagtaaaat ctttcttcac
     6241 aatattacgg gtattaagag caagttcaat accgcgattt tctgtttcag cagcattctt
     6301 gttcatttta taagtagtgc tacctgtgta attacccata atagatgtcg gcaatgcctg
     6361 cgaaagaatt acatccgttg tttttgtccg atacaaatcc accgtcagat caatacgatt
     6421 attgaggaag ctggcgtcaa taccaatatt caagtcatga gtcttttccc atgtcagatt
     6481 acggttagtg atatactccg taaacttggc tgacggaaca gtcattcctc ccaaagaagt
     6541 tgtcacattt tccaaagatg ccatagaact gtattcagca ataccggcag tagttccggc
     6601 aacaccatag cctacacgca gtttcaagtt atccaaccag ctttttgtac cagccataaa
     6661 tgcttcatcc gagattctcc agcctacaga aactgccggg aataaattcc atctattgtc
     6721 tttcgacaaa cgggaatccg cttcataacg actggaagca gagaacaggt atttaccttt
     6781 gtatgagtag ttgacacgac cgataaagcc catccctttc gacatcagat atttgctgtt
     6841 cagtttctta ttatcagcat tctgcaatgc ataccacaac atatcattgg tcgccgggtt
     6901 ttcaccatag acataatatt cttcactctg attgtaattc caggaagtaa cccctgtaac
     6961 cgtcaggtca tgatccttat taaatgtatt gtgataagtt actatgtttt cccatttgta
     7021 attatatccc gtcttattgg tagcttcgcc atatgccacc gtactgcctt tcaacacatt
     7081 ataagagcct tctcccataa atttaccggc acgagattga gaataactgc ctccaagctg
     7141 tgacttgaca gacaatcctt ttaccggttt ccattcaaga taagcgtcaa cataagccct
     7201 tcctgcttta gtattattct tatatactcc atcttgttcg tctgctaacg gatttggatc
     7261 tgatgatgaa ccgggaatcg gaaattccgt aatagagcca tcttcattat aaggaacacc
     7321 caatggggta gcaaacaatg ctctttctaa aacggcatta cgtgaattct tgttggagaa
     7381 agatgcctga acattaatac cggcattcat ccatttattg atttcctgat ctatacggac
     7441 acgggtagag tacactttat agtcatcatt ctcatacata ctcttctcat cagtatagtt
     7501 caacgagaaa taagctttcg tcttctcagt acctccggca acggaaatac tatagttctg
     7561 cttcaagcct gaatgtaata cttcatctcc ccaatttacc cattggttgt ttttgatggc
     7621 gtcgtatctt tcaccgaaaa tttcctcctc attagtagta ccgacaggct tataagaatc
     7681 ttttatataa ttaatgtatg agtcacccgt acgcatctta ggaactcggg cacccatagt
     7741 cgttccggca taagcattaa aattgatgac cggttttcca ctcttggatc ctttcgtagt
     7801 cacgataata acaccattcg caccacttga accgtagata gccgtagaag atgcatcttt
     7861 cagtacttcg atagattcaa tatcattggg attcaaggta gcaatatcac ccggcatacc
     7921 atcgataata taagtcggct taaatgcatc tgaagagata ttaccatcct catccaactg
     7981 taaagagcgg gtaccacgca actgaactgt cggtgacgaa cctgcctgac ctgaagaatt
     8041 agtgatatcc agacctgcaa ttttaccttg cagcgcatcc atcggattca cgcccggatt
     8101 catggtgata tcctctgatt tcacagaagc aaccgcacct gtcaaatcac gtttctttac
     8161 cgtaccgtaa ccgatgacca ctacctcatc caaagcctgt gtatccggaa ccatctttat
     8221 attcagcggg gcatttccgc tcacaatgat agcctgttct ttatatccga tataagaaac
     8281 aaccagttct ttacttccgc ccggaagatt aatggagaac ttaccgtcaa aatcagttat
     8341 cgtacctgta cttgtacctt tcaccacaat agaaacaccg atcaacggtt ctccattttc
     8401 atctaaaaca gttcctttca ctgtctttgt ctgcatgacg gcttgcacag taatatcatt
     8461 cacaccattt tctgcataaa cagatatggc agaaggggca cacaataaag tcagccctaa
     8521 aatcatggaa acgcgtctat gctgtgtctt ccacgcttca acaaatgcat tctctctcat
     8581 tcgttttaat ttaaagttaa tattaatttt cctttgttta gtaaaataca attagcataa
     8641 tcttttacat agacgtttcc attttacttc tgtactcaca aaggacgaca ataatacaaa
     8701 aattcgcttt tccaagcctt tttttcctca aatatatgca aaggataaaa agacaaattt
     8761 taggctaagc caacaaattc tcttttttat tgttgaatga agaataaaaa gtgtaaattt
     8821 gtatttggat gccacttaaa ctaacaacat attatcgcgg aaaagacatt cctgatttac
     8881 cgggaaccaa tacttttcat tccaaggagt tgtttcagat ctatgaatcg actccgggat
     8941 ataccccgct actgatagtg gcaacagaag aaggcagacc tgtagcacgc cttcttgccg
     9001 ccatacgcaa gaccaaaaag tggcttccct cctctcttgt caaacaatgt gtggtatatg
     9061 gtgcaggtga atttctggac acttctctac cggcaagtaa agagaaggag gaagaaattt
     9121 tcggtgaaat gctggagcat ctcacacaag aagcatcacg cacctgcatt cttattgaat
     9181 tccgcaatct ggataattcc atgttcggtt accgtttctt ccgaaagaac gacttttttc
     9241 cggtcaactg gctgcgtgtc cgtaactctc tgcatagtac gcagaaagcg gaagaccgtt
     9301 tcagcccgtc acgtatccgt cagatccgga aaggactcaa aaacggagcc aaagtagtag
     9361 aagcccatac ggtggaagag attaaggaat tctcgcggat gctgcacaaa gtttattctt
     9421 cacgaatacg gagatatttc cctgccaatg actttttccg ccacatgaac agcatgctta
     9481 tcagaggaaa acaggcaaaa atattcatcg taaaatacaa ggaaaagatt atcggcggct
     9541 ccgtctgcat ctattccggc gaaaatgctt ttatctggtt ttcaggagga atgcgaaaga
     9601 cgtatgcact gcagtcaccc ggcatactgg cagtatggaa agcacttgaa gatgcccatg
     9661 aacggggatt ccgtcacatg gagttcatgg atgtaggact tcccttccgc aaacatggtt
     9721 atcgtgattt cgtactccgt ttcggaggca aacaaagcag tacccgccgt tggttccgta
     9781 tcagctggcc atggctgaat aatcttctgg tcaagtttta tgtctaaatg taatctcaag
     9841 attacaaaac ccgtctttgt cacccatccg gagcattttt tatgctttat tccccaataa
     9901 ttaaatgcag aaatatttct ttattagaca agaaatgtct ttctttgtgc tgttttatgc
     9961 catataagca cataattaaa ccatataaaa aaatatagtt atgaagattt cacacattga
    10021 gcatctgggc attgctgtaa aaagcatcga agaagccctt ccttactacg aaaacgtatt
    10081 gggtctgaaa tgttacaaca tcgaaacagt agaagatcaa aaagtaagaa ccgctttttt
    10141 aaaggtaggt gaaacaaaaa tcgaattgtt ggagccgact tgccccgaaa gcaccattgc
    10201 taaatttatt gaaaacaaag gtgcaggtgt tcatcacgta gcatttgccg tagaagatgg
    10261 cgtagctaat gctttggcag aagcagaaag caaagaaatc cgtctgattg acaaggctcc
    10321 ccgcaaaggt gccgaaggtc tgaacatcgc tttccttcac ccgaaatcaa cactgggtgt
    10381 gctgacagaa ctctgcgaac actaatcata aactctaaaa aaacaacaag aactcatgag
    10441 taatcaactt gaaaaaatta aagagcttat tgaacgccgt gccgtagcac gtatcggagg
    10501 cggtgaaaaa gcaattgcga agcaacacga aaaagggaaa tacacagcac gcgagcgtct
    10561 ggctatgctg cttgacgagg gtagcttcga agaaatggac atgttcgttg agcacagatg
    10621 cacgaacttc ggcatggaga aaaagcacta tccgggtgat ggtgtagtaa ccggttgcgg
    10681 tactatcgaa ggtcgtctcg tatatctttt tgcacaggac tttaccgtta ctgcaggttc
    10741 actgtcggaa acaatgtcac tgaagatctg taagattatg gatcaggcaa tgaagatggg
    10801 tgctccttgt atcggtatca atgactcggg cggtgcacgt atccaggaag gtattaacgc
    10861 tttggcaggt tacgcagaaa tcttccagcg caatatcctc gcttcgggtg tcattccgca
    10921 gatttccggt atcttcggtc cttgtgccgg cggtgctgtt tattctccgg cactgacaga
    10981 cttcacactg atgatggaag gtacttctta tatgttcctt acaggtccga aagtagtgaa
    11041 aactgtaacc ggtgaagacg taagccagga aaacctcggc ggagcaagcg ttcactctac
    11101 caaatcgggt gttactcatt tcactgccaa aacagaagaa gaaggtctgg cattgatccg
    11161 cacacttctg agctatattc ctcaaaacaa cctcgaagaa gctccatacg tggattgcac
    11221 agatccgatc gaccgtctgg aagattcttt gaacgatatt atccccgaca gccccaacaa
    11281 accgtatgat atgtacgagg ttatcagcgc tatcgtggac aacggtgaat tcctggaaat
    11341 ccagaaagac tatgccaaga atatcattat cggttttgcc cgtttcaacg gtcagtctgt
    11401 aggtattgta gccaatcagc ctaaattcct tgccggtgta ctcgacagca acgcatctcg
    11461 caaaggcgca cgtttcgttc gtttctgcga tgctttcaat atccctatcg tgtcgttggt
    11521 agacgtaccg ggattccttc cgggaacagg ccaggaatac aatggcgtta tccttcatgg
    11581 tgccaaactg ctgtatgctt acggtgaagc tactgtacct aaagtaacaa tcacattgcg
    11641 taaatcttac ggtggttctc acatcgtgat gagttgtaaa caacttcgtg gtgacatgaa
    11701 ctatgcttgg ccaacagctg aaatcgctgt aatgggtggt gcaggtgcag tagaagtatt
    11761 gtacgcgcgt gaagccaaag accaggaaaa tccggctcag ttccttgccg aaaaagaagc
    11821 agaatataca aaactgtttg ccaatcctta taatgcagct aagtatggtt acatcgatga
    11881 tgtcatcgaa ccccgtaaca cacgtttccg cgtgatccgt gctttgcagc agttgcagac
    11941 taagaaactg tctaacccgg cgaagaaaca tggtaatatt ccgttgtaat ccaacttttc
    12001 acattaaaac aagaacgatt atgaacaaaa ccaaaatcgg aatattcctt tctctgctgt
    12061 tgctgattgg attgacttcc tgcggagagc aaaagtcgaa caataagcta ttgctgaatg
    12121 aagtcctgat agacaatcaa aacaactttc aggatgacta cggcttgcac agtgcatgga
    12181 ttgaaatatt caacaaatca tacggtagcg cagacttagc agcttgttta ttgaaagtaa
    12241 gcagccagcc gggcgataca gtaacttatt ttatcccgaa aggtgacata cttacattag
    12301 taaagccacg ccaacatgcg ttattttggg cagacggcga acctaatcgt ggtaccttcc
    12361 ataccagctt caaactgaat cccgaaacag ccaattgggt cggtcttttt gattcaggca
    12421 gaaagctact tgatcaagtt gtcataccag caggaacttt aggtcccaac cagtcttatg
    12481 cacgcataag cgacggagca gcagactggg aagtaaaagg cggaagcaaa gacaaatacg
    12541 taaccccgag cacgaacaac aaaacccttg acagtaatgc caagatggaa aagttcgaag
    12601 aacatgactc agtcggtatc ggtatgtcta tttcagccat gagcgtagta ttctgcggat
    12661 tgattcttct cttcattgcc ttcaaggtgg taggtaaagt tgctgtcaac ctaagcaaac
    12721 gcaatgcgat gaaggctaaa ggcatcgata aagtcgaagc gaaagagctt tcacaggctc
    12781 cgggtgaagt ttatgctgct atttcaatgg cattacacga aatgcaggat gaggtacacg
    12841 acgtagaaga aacggtgctg accatcactc gtgtgaagcg cagttattca ccgtggagtt
    12901 cgaagattta caccttgcgt gaaaatccta atagaaagta aatcattccc taaaaagata
    12961 aaacgatgaa agaatataaa tataaaatca acggtaactc atacaaagta accatcggag
    13021 atattgaaga taatatcgct catgtagaag tgaacggtac gcattacaaa gtagaaatgg
    13081 agaaacaacc gaaagctact cccaaaccgg cagtagtacg ccctatgcct aactcaccgg
    13141 ctgctcctac cacaccggta gtaaaaccgg ctgctccttc taccggcaaa tcaggcgtta
    13201 agtccccact gccaggcgtt atccttgaca tcaaagtaaa tgtaggtgat acagtgaaaa
    13261 gaggacagac tatcattata ttggaagcca tgaagatgga aaacaatatc aatgcagaca
    13321 aagacggtaa agtgactgcc atcaatgtca acaagggaga ttctgttctt gaaggtaatg
    13381 acctcgtaat tattgaataa tatgggagat tttattaact ttttaggaaa taaccttgcc
    13441 gatttctgga cctacacggg atttgccaac gcgacagttg gtcatatcgt gatgatcctc
    13501 gttggcttgt tcttcatcta tttagctgta gccaaagagt tcgagccgat gttgttaatt
    13561 cctatcggat tcggtattct gatcggtaat atacctttca atatggacgc cggactaaaa
    13621 gtcggtatat atgaagaagg ttctgtattg aacatattgt atcagggagt aacgtccggc
    13681 tggtatccac cgctcatctt cttaggtatc ggtgccatga cagacttctc ggcgctaatc
    13741 tccaatccga agctgatgct gatcggtgcg gctgcccagt ttggtatctt cggtgcatat
    13801 atgatcgcat tggaaatggg atttgacccg atgcaggccg gtgctatcgg tatcatcggt
    13861 ggagcagatg gtccgacggc tatcttcctt tcgtccaagc tggcacctaa cttaatggga
    13921 gccattgcgg tatccgccta ctcctatatg gcgttagttc cggtgataca gccgcctatc
    13981 atgcgtctgc tcaccaccaa acacgaacgc gttatccgta tgaaaccgcc acgtgccgtt
    14041 tctcatacag agaaagtgat tttcccgatt atcggtctgt tgctgacctg cttcctggtt
    14101 ccttccggtc tgcctctgct gggtatgctg ttcttcggta acctgttgaa agaaagtggt
    14161 gtaacccgtc gtctggctaa cacagccagc ggtccgctga ttgacgttat cactatcctg
    14221 ttaggtttga cagtaggtgc ctctactcag gcttccgagt tcctgacact cgactccatc
    14281 aagatctttg ccctcggtgc actgtcattt atcattgcta ccgcatcagg tgtgatcttc
    14341 gttaagatct tcaacctctt cctcaagaag ggcaataaga tcaatccgtt gatcggtaat
    14401 gcaggtgtat ctgccgttcc cgactctgca cgtatctcac aagttgtcgg tctggaatat
    14461 gatccaacca actacttgct gatgcacgct atgggtccga acgtagcagg tgtaatcggt
    14521 tcagctgttg ctgccggtat cctgttagga ttcttaatgt agggacaagg tgcccctgtc
    14581 aggcttccat tcggaatgcc gggacaaaat attaggtgcg gatattgctg ttttagatgc
    14641 aatatccgca cttatcatta tgttatcttc tcttatcatt ccgttaatct attccaaaaa
    14701 ggctacgaat aattcgtaaa tacgatattt tttagcatct tgcgtctttg ttacgacaca
    14761 ctaaaataaa tatttatgaa acggattatt ctcctcgcct tcctttgtac caccaccctg
    14821 gtgatggcgc agcgcatcaa tcatgttcag gaagcaatag caaactacga ttacgaggca
    14881 gctctcactc tcattgccaa agaaaagccg acaattcccc tactcttgca aaaaggtaaa
    14941 gcacaacggg gacttgggat gaatacagaa gcactttcca cttatcagga aatcataacc
    15001 aatgacactg ccaatacccg tgcatatatc gaagcagccg aatgctgccg ttcattggcc
    15061 aaataccaac aggcactgaa atattatgaa caggcactcg atctgaatcc ggaaaacaaa
    15121 tatgtgcgta ttcaatacat cggactactg ctctctttac agaagtttca ggatgcttta
    15181 ggagaaagta gtctgatgac agagaaagac agttccgcca tcgccttgca tctgcaggcc
    15241 caaagtttcg aaggtatggg agagcttctc cccgccacgg ggtgctacta taatatacag
    15301 gagaaatacc ccgacgacta tcttgccgcc gccaagctcg ccgcactgaa catagccggt
    15361 tcttatttca acgaagcaat tgaagccacg gagaaatatc ggcagattga caccaccaac
    15421 atcgctgtca accgccagaa tgcactggca tattgtctga ataaggatta tcctaccgct
    15481 atccagcgtt atgaatacct ggtgagccag ggcgacagtt cttttcatac ctgttattat
    15541 ctgggtatca gctattatgc ggaagagaaa tattacgaag cacatgactt tctggaagcc
    15601 gcccgaaaac atgatcctga aaacgtaaac ctgctttatt atctcggccg tgcctgcgcc
    15661 aagacttcat ggaagaagtt aggcgtagag tatctcgaaa aagcgcttga tctgactatt
    15721 cccaaagaca gtaatatggt acggctctat attggcatga ccgactgcta caaaatggca
    15781 cagatgccta aagagcagat tgaatcgatc agagaacgct accggaaata cgataaacaa
    15841 aatcataagc tgctctatga tatggctttc atttatttct attcgctgaa agacaagaaa
    15901 aacaccgaac gctgtctgga agcattctta aaaacacgcc cgaaagaaga taaggaagaa
    15961 gaagcgaaac taaacgaacg aggcgaactc gttttaggca ctaagaatta ttataatgct
    16021 gcagctaact ggctgaaaga cattcaaagt aagcaaaaga tagaagattt cttcttggga
    16081 ggaaatgtgc ccgctcagaa accggaataa gcagatagag aatattacgt aaaaaggctg
    16141 caaccgtcaa acgattgcag cctttctata taataggata atctgtgatt attcagccaa
    16201 tttgttgtct gaacccagca cattaaggat tttgtgcttg tacagttttt ccatgttgtc
    16261 acgagccggg ccaagatact ta
//
