LOCUS       c00227_gnlPro..         3976 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EGIBHFOI_227.
ACCESSION   c00227_gnlPro..
VERSION     c00227_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 18:21:23
            Original ID  :: gnl|Prokka|EGIBHFOI_227
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3976
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3976
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[284:3255]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      285..3255
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3976
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3976
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             285..2513
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg227_1"
                     /sec_met_domain="Gly_radical (E-value: 3.1e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1174.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLRRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     KFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2527..3255
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 71.7;
                     E-value: 2.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg227_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.1e-29, bitscore:
                     92.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKTLVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(3248..3697)
                     /locus_tag="ctg227_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(3684..3851)
                     /locus_tag="ctg227_4"
                     /transl_table=11
                     /translation="MKEIVNRILDVIMYLIPFFGKRKRDKVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             complement(3911..3976)
                     /locus_tag="ctg227_5"
                     /transl_table=11
                     /translation="DKNPGSGGDGSGDEEAPDPTV"
ORIGIN
        1 acggttttca agaccgccgc aatcgaccac tctgccacct ctccaaaact tccttatcag
       61 aagtgctttt cgttaaaagc ggtgcaaagg tacaaagata ttttaaaccc gcaagagttt
      121 tcactaaaaa tttaatctaa tcttttccta aggatagcaa ttaataggta ttttatgttg
      181 aaaagcataa aatagagcat atttatgccg attatatgaa aaatgattgt aactttgtgc
      241 gctctaaaga ataggataac tacaacctct aattttatca ttttatggaa ttaaacaaga
      301 tctttaaaga cggtctttgg agcagcgaaa tcaacgtcag agatttcgta agtcataaca
      361 tcactccgta ttacggagat gcttcattcc tcgaaggacc tacagaacgt accaaagccg
      421 tatggaaccg ctgcctcgaa gcgttggcag aagaaagaga aaacaacggt gtccgctcat
      481 tagataatgt taccgtatca accatcactt cccacaaggc cggatatatc gacaaggaaa
      541 acgaactgat cgtcggcctg cagacagacg aacttctgag acgtgccatc aagcctttcg
      601 gaggtatcaa cgtagtcagc aaagcctgtc acgaaaacgg tgtggaagtg gacgaccgcg
      661 taaaagatat tttcactcac taccgcaaga cgcacaacga cggagtattc gacgtgtata
      721 cggaagaaat ccgttccttc cgttcgctgg gattcctcac cggacttccc gacaattatg
      781 cacgcggacg tatcatcggt gactaccgcc gtatggctct ttacggcatc gaccgtctga
      841 tcgaagcaaa gaaggaagat ttgcataacc tcaccggtcc gatgacagag gcccgtatcc
      901 gtctgcgcga agaagtggca gaacagatca aggcactgaa agacatgaaa gtgatgggcg
      961 aatactacgg tctcgacctg agccgtcccg cttacacggc acaagaagcc gtacagtggg
     1021 tatatatggc ttaccttgcc gccgtcaaag aacaagacgg tgccgccatg tcactgggta
     1081 acgtttcttc tttcctcgat atctatctgg aatatgaact gagcaaagga accatcaccg
     1141 aatcgttcgc gcaagagctg atcgaccagt ttgtcatcaa actgcgtatg gtccgccacc
     1201 tgcgtatgca atcatacaac gacatctttg caggcgaccc gacctgggta accgaatctc
     1261 tgggcggacg tctcaacgac ggacgtacca aagtgacaaa gacctccttc cgtttcctgc
     1321 agacattgta caacctcggc ccttcaccgg aaccgaacct gaccgtactc tggagcccgg
     1381 aacttcccga aggattcaaa gaattctgtg caaaggtttc tatcgacact tcttctatcc
     1441 agtacgaaaa cgacgacctg atgcgtgaag tacgtcagtc cgacgactac ggaatcgcct
     1501 gctgcgtatc ataccaggaa ataggcaagc agattcagtt cttcggtgcc cgttgcaacc
     1561 tggccaaagc cctgttgctt gccatcaacg gcggacgttg cgagaatacc ggtacagtca
     1621 tggtgaagaa catccccgta ctgaccagtg acacgctgaa gttcgaagaa gtaatggaca
     1681 actacaagaa agtgttgacc gaaatcgccc gcgtatacaa cgaagcgatg aacatcatcc
     1741 actatatgca cgacaagtat tactacgaga aagcccagat ggctcttgta gatacgaacc
     1801 cgcgcataaa ccttgcttac ggtgttgccg gactttccat cgcactcgac tcactgtctg
     1861 ccatcaaata tgcgaaagta actgcccgcc gcaacgatat cggcctgaca gaaggtttcg
     1921 acatcgaagg agaattccct tgcttcggta acgacaacga caaggtagac caccttggcg
     1981 tagacctggt atacttcttc agcgaagaat tgaagaaatt gccagtttac aagaatgccc
     2041 gtcctaccct ctccttgctg actatcactt ccaatgtgat gtacggcaaa aagaccggtg
     2101 ctactcccga cggacgtgcc aaaggtgttg ccttcgctcc gggtgccaac ccgatgcacg
     2161 gacgcgacaa gaatggtgcg atcgcttctc tgagctctgt agcgaaactt cgttaccgtg
     2221 actcacagga cggtatcagc aacaccttct ctatcgttcc gaaatcactg ggtgctacgg
     2281 acgaagaccg tatcgagaac ctcgtaacga tgatggacgg ttacttcacc aaaggtgctc
     2341 accacctgaa cgtaaacgta ctgaaccgtg atatgctgta cgatgccatg gagcatccgg
     2401 aaaactatcc gcagctgact atccgcgttt ccggctatgc cgtaaacttc gtgaagctga
     2461 gccgcgaaca ccaactggaa gttatcagcc gtagcttcca cgaacgtatg taatctatta
     2521 ctctatatga tgataaacgt acattcatac gaaagtatgg gaacattcga cgggccgggc
     2581 ttacggctcg tcgtttttct tcaaggatgc aacttccgct gcctctattg cgccaatccg
     2641 gatacgatag caggaaaggg aggcacgcct accccaccgg aagaaatcgt ccgcatggca
     2701 atgagccagc gccccttctt cgggaaacga ggaggaatca ctttctccgg aggcgaaccg
     2761 acgtttcagg cgaaaacact cgtcccgctg gtccgcgaac tgaaagaaaa aggcatccat
     2821 gtctgtctgg acagcaacgg aggactttgg aacgaagacg tagaagaatt attcagactg
     2881 accgatcttg tgttattgga tataaaggaa ttcaacccca accgccatca gacgctcacc
     2941 ggaagaagca acgagcaaac catccgcacg gccgcctggc tggaagaaca ggagaaacct
     3001 ttctggctac gctacgtgct agtgcccgga tacagcgatt ttgaagaaga catacgcgca
     3061 ctgggagaag cactcggaaa atacaagatg atccaacggg tggaaatact gccttaccat
     3121 acactgggcg tacataagta cgaagcgatg ggacaggaat acaagatgaa aggggtaaaa
     3181 gaaaataccc ccgaacagct tgaaaaagct gcggaggtat tcaaagagta tttcactacg
     3241 gtggtagtta attaatggag aattctccaa tgacctcaaa gcaagggcat tctttaatcc
     3301 gttcccaagg atctacgatc ccattatagt tggtgtcggg acttaaatcc cgatgaccta
     3361 ctaccttagc ttccggataa cgctgcaaca attcgtgtac caaacgatgt aacgcctcct
     3421 tctgacgggg tgtacgcgta tccgccgctt ttccattctc atccaaaccg ccctcatagc
     3481 atacacctaa actgatcgca ttgtagccac gtgcatgagc cccgaccgta tcttcatccc
     3541 gcatcggctc aatctcgccc gacttgcgta tataataatg atagccccag cacgaaaagc
     3601 cacgataacg gtgagccgta tccacatcct ttgccgtaaa gtcgcaatcg acacgggtgg
     3661 cggaacaatg gatcacgatc agactaatct ttctcatgtt ccttttccct ttccgtcgtt
     3721 ttcactttgc acacctcctt acaagtagta tgatagcgca cttctctcac caccttgtcc
     3781 cgctttctct ttccgaagaa aggaatcaga tacatgatca catctaaaat ccggtttact
     3841 atttctttca ttttatcaaa atattaatgg aagttattct aattcgttat taccttgacc
     3901 cgtctgccaa ttataccgta gggtccggag cctcttcatc gccggagcca tcaccgccgg
     3961 aaccggggtt cttgtc
//
