LOCUS       gnlProkkaLDJOJKIP_409   5750 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LDJOJKIP_409.
ACCESSION   gnlProkkaLDJOJKIP_409
VERSION     gnlProkkaLDJOJKIP_409
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-01 13:39:40
            Original ID  :: gnl|Prokka|LDJOJKIP_409
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5750
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5750
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[1317:5631]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1318..5631
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..5750
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..5750
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             106..1254
                     /locus_tag="ctg409_1"
                     /transl_table=11
                     /translation="MIEKQSYQESFSEVDKRKDDEMEIDLMAIFYKIITVRGVLCKAAG
                     VGVIIAIVVALSIPKQYTVKVTLSPEMGNAKGSNGLAGLAASFLGDGALIGESTDALNA
                     SLSADIVSSTPFLLELLEMEVSASKKDDKMTLESYLDEESSPWWDYLIGFPGIVIGGVK
                     SLFGEDTISADGGRRGTIELTKEVNEKINFLKKKISASIDKKTAITSITVTLQNPQITA
                     VIADSVVHKLQEYIIGYRTSKVKEDCAYLERLFKERQQEYYAAQRKYADYVDAHDNVVL
                     QSVRAEQERLQNDMSLAYQIYSQVANQLQVSRAKVQEEKPVFAVVEPAVVPLKPSGMGL
                     KIYILLFVFLSVSGTLVWVFFGKSLLASLRKELKNKEIEQDK"
     CDS             1318..2037
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 54.5; E-value:
                     1.6e-16)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg409_2"
                     /sec_met_domain="NTP_transf_3 (E-value: 7.5e-17, bitscore:
                     50.8, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRTAMIMAAGMGTRFGQYTELIPKGFVKVGGIPMIVRSIDTLLS
                     CDIERIVIGTGYKQEVYEELKTDYPMLETCFSPRYAETNSMYTLYNTREILGNDDFLLL
                     ESDLIFEKQAIMSLLECPAADAMLITPVTKFQDQYYVEHDDNFRLSSCSVNKNKLNAKG
                     ELVGIHKLSGSFYKIMCADYASIVDEQPNLGYEYELLRISCSQSPVYVHKVEGLKWYEI
                     DDISDLEYAEKYIVPYC"
     CDS             2058..3359
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 208;
                     E-value: 2.8e-63)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg409_3"
                     /sec_met_domain="CTP_transf_like (E-value: 9.2e-14,
                     bitscore: 40.6, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 1.8e-121, bitscore:
                     393.3, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 1.7e-63, bitscore:
                     203.0, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKTVYIGMTADIMHPGLIRIINEATKYGDVIIGLLTDKAIAEHK
                     RLPYLTYEQRKEVVQNIKGVCKVVPQEEWSYVENLKRIKPDYIIHGDDWKTGPLREERV
                     RVFEVMNEQGGKVIEIPYTLGINSSSLDKDIKAIGTTPDVRLKSLRRLINAKPVVRILE
                     AHDGLCGLIIENQEILKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAIIIEDKVGLKKNSLFGTDVIQTQDT
                     IEGFCNKIKAGKASQITDDFMIIARIESLIAGKPVSDALERAFAYVRAGADGIMIHSKN
                     KSGEDIKEFCLAFRKQYAHVPIVVVPTTYDHIYESELCDWGVNIIIYANHMLRAAYPAM
                     MNVAKIILENERALEVRELCMPIKEILELIPGTK"
     CDS             3376..4500
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 61.4;
                     E-value: 1.2e-18)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg409_4"
                     /sec_met_domain="TPP_enzyme_N (E-value: 5e-21, bitscore:
                     63.7, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.2e-21, bitscore:
                     65.8, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSVEKVYETFLSHDVDFFTGVPDSLLKNICAYITDHTSREKHII
                     AANEGAAVGIASGYYMASGKVPVVYMQNSGLGNTVNPLLSLADEQVYSFPMLLMIGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMRIPYIILDSNENDAFIQLHDIIQSAKTKNIPHAII
                     IRKDVFGKYKLQKEFGCNYPLSREDALQIVVDYLPENSVVVSTTGKLSRELFEYREMKE
                     QGHEHDFLTVGSMGHSSSISLGIAIAKQDRPVYCLDGDGAFIMHLGAISNIGDLSPKNY
                     YHILFNNGAHESVGGQPTLGFSLDIPAIARGSGYKHTYTVCTKVEIEEAMKQLPKLCGP
                     VLLEIKVKIDSRENLGRPTTTPIENKEHFMDFLK"
     CDS             4510..5631
                     /NRPS_PKS="Domain: Aminotran_5 (30-344). E-value: 5.1e-34.
                     Score: 109.6. Matches aSDomain:
                     nrpspksdomains_ctg409_5_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 77.2; E-value:
                     2e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg409_5"
                     /sec_met_domain="Aminotran_5 (E-value: 1.5e-31, bitscore:
                     98.2, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIERKILLNPGPATTTDSVKMAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGNLEKHTAVLFCGSGTINIDICLNSLLPEDKRVLIVNNGAYSTRAVEVCQYY
                     GLPHINLEFSVYERPDLSVVENVLQENPDIAMVYTTHHETGTGILNPIREMGDIAHKYQ
                     AKFVVDTTSSLGMIPFDIEKDNVDFCMASAQKGLQAMTGLSFIIGNEEQIKVSKKYPKR
                     SYYCNLYLQYENFERTGEMHFTPPVQTIYATRQALNEYFEVGEEAKFARHKRVFEAIHS
                     GINEIGLKSVIKREWQSGLVVSVQYPEDPNWDFEKIHDYCYERGFTIYPGKISTANTFR
                     ICALGEIEVDDIENFFKVLKAALNHYNVTLPMS"
     aSDomain        4600..5541
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg409_5_Aminotran_5.1"
                     /evalue="5.10E-34"
                     /label="ctg409_5_Aminotran_5.1"
                     /locus_tag="ctg409_5"
                     /protein_end="344"
                     /protein_start="30"
                     /score="109.6"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGNLEKHTAVLFCGSGTINIDICL
                     NSLLPEDKRVLIVNNGAYSTRAVEVCQYYGLPHINLEFSVYERPDLSVVENVLQENPDI
                     AMVYTTHHETGTGILNPIREMGDIAHKYQAKFVVDTTSSLGMIPFDIEKDNVDFCMASA
                     QKGLQAMTGLSFIIGNEEQIKVSKKYPKRSYYCNLYLQYENFERTGEMHFTPPVQTIYA
                     TRQALNEYFEVGEEAKFARHKRVFEAIHSGINEIGLKSVIKREWQSGLVVSVQYPEDPN
                     WDFEKIHDYCYERGFTIYPGKISTANTFRICAL"
     CDS             5628..5750
                     /locus_tag="ctg409_6"
                     /transl_table=11
                     /translation="MIAEILLKIRHFYFSLRGIHFHPESVISGVPIRSFRLVGKY"
ORIGIN
        1 agaaagggaa catagctaat attttaggtt atgctacttc cttcagttct ttgggaatga
       61 tgattgcttc cattgctaat ttgataaaga aataaatgac ctgttatgat tgaaaagcaa
      121 tcttatcaag aatcgttttc tgaagtggat aaaaggaaag atgatgaaat ggaaatagac
      181 ttgatggcta ttttttataa gattattact gttcgtggag tactatgcaa agctgctgga
      241 gtgggagtaa taattgctat agtggtggca ttgagcattc ccaagcaata tacagtaaag
      301 gttacccttt ctcctgaaat gggtaatgcg aagggaagta atggtttagc cggacttgct
      361 gcctcgtttt taggagatgg ggctctaata ggggaaagca cagatgcctt gaacgcttct
      421 ctttcagcag atattgtttc ttcaactcct tttttattgg aacttttaga aatggaagtc
      481 tccgcttcta aaaaggatga taaaatgact ttggagtctt atctcgatga agagtcatct
      541 ccttggtggg attatcttat aggttttccg ggcatagtaa tagggggagt taaatcatta
      601 ttcggagaag atacgatttc tgccgatgga gggcggcgag gaactatcga attgacgaaa
      661 gaagtaaatg agaagattaa ttttcttaaa aagaagatta gtgcatccat tgataagaaa
      721 accgccataa cgagcattac tgtcacctta caaaatccgc aaatcactgc ggttatagca
      781 gattctgtcg ttcataaatt acaggaatat attattgggt atcgaacttc aaaagtgaaa
      841 gaagactgtg cgtatttgga acggttgttt aaagaacgtc agcaggaata ttatgctgct
      901 caaagaaaat atgcggatta tgtagacgcc catgataatg tggttcttca aagtgtacgt
      961 gctgagcagg agcgtttaca gaacgacatg agtttggctt atcagatata tagtcaggtt
     1021 gctaatcagt tgcaggtatc cagagccaaa gtacaggagg aaaagccagt ctttgcagta
     1081 gttgagcctg ccgttgtacc tttgaaacct tcaggtatgg gactcaaaat ttatatttta
     1141 ttatttgtgt tcctgtccgt atctggtact ctcgtgtggg tgttttttgg caaaagtctg
     1201 ctggctagct tgagaaaaga actgaaaaat aaggagatag aacaggataa atgatgctaa
     1261 aacgtcgttt acaacattct tacatcaatt aaattcactt actatattat taaacgaatg
     1321 atacgtacag ctatgataat ggctgcgggc atgggaaccc gttttggcca atatacagaa
     1381 ttgataccta aagggttcgt caaagtggga ggtattccaa tgatagttcg ttctattgat
     1441 actttgttat catgtgacat tgagcgaatt gtaatcggta caggatataa acaagaagtt
     1501 tatgaagaat tgaagacaga ctatcctatg cttgaaacct gttttagtcc tcgttatgct
     1561 gaaacaaata gtatgtatac tttatataat acgcgggaga ttctgggcaa tgatgatttt
     1621 ttattgttag agtcagattt gatttttgag aaacaagcaa taatgagctt attggagtgt
     1681 cctgcggcag atgcaatgct aataactcca gtgacaaagt ttcaagatca gtattatgtg
     1741 gaacatgatg ataattttcg cctttcttca tgttctgtca ataagaataa actaaacgct
     1801 aaaggcgaat tggtcggcat ccataaacta tccggttcct tttacaaaat aatgtgtgct
     1861 gattatgcga gtattgttga tgaacaaccc aatttgggat atgagtatga attactacga
     1921 atatcttgtt ctcaatctcc tgtttatgtg cataaagtag aagggctgaa atggtatgaa
     1981 atcgatgaca tatcagattt ggaatatgca gagaaatata ttgttccgta ttgttaatta
     2041 aaataatata tcaaattatg agtaagacag tttatattgg gatgactgcg gatattatgc
     2101 atccaggact tatccgcatt attaatgagg ctactaaata cggtgatgtt ataattggtc
     2161 ttcttacaga taaagctatt gcggagcata aacgccttcc ttatctgacc tatgaacaac
     2221 gaaaagaggt agttcagaat attaaaggcg tatgtaaagt tgttcctcaa gaagaatgga
     2281 gctatgttga gaatctcaaa cgcattaaac ctgactatat aattcatggt gacgactgga
     2341 aaaccgggcc tttacgtgaa gaacgtgtgc gcgtatttga agtgatgaat gaacagggag
     2401 gaaaggttat cgaaattcca tatactttgg gaattaattc atcttctttg gataaagata
     2461 ttaaggctat aggtacaacc cctgatgtac gtttaaaatc acttcgtcgt ttgataaatg
     2521 ccaaacccgt agttcgtatt ttggaagctc atgatggact atgtggcttg ataattgaaa
     2581 atcaggaaat attaaaaggg gataagaggg aagtatttga cggaatgtgg tctagtagcc
     2641 ttacggattc aaccagtaaa ggtaaacctg atattgaggc ggttgattta actacccgat
     2701 tgcaagattt gaataatatt ctggagtgta ctactaaacc tattattttt gatggagata
     2761 caggtggtaa aattgaacat ttcgtattta cagtacgcac tttagaacgt catggaattt
     2821 cagctataat aattgaagat aaagttggat taaaaaagaa ttcattgttt ggtacagatg
     2881 ttattcaaac tcaagataca atcgaaggtt tctgtaataa gatcaaagca ggaaaggcat
     2941 ctcagattac tgatgatttt atgattatcg ctcgtattga gagccttata gcgggaaagc
     3001 cggtaagtga tgctttagag agagcttttg catatgtccg agcgggagcg gacggtatta
     3061 tgatacatag caaaaataag tcgggtgaag atataaaaga attttgtttg gcgtttcgaa
     3121 aacagtatgc tcatgtacct atcgtggttg tgcctactac atatgatcat atttatgagt
     3181 ctgaattgtg tgattggggt gtgaatataa ttatttatgc taatcatatg cttcgtgccg
     3241 cttatcctgc tatgatgaat gtcgcaaaga taatattaga gaatgaacgt gctttagagg
     3301 tgcgcgaact gtgtatgccc ataaaagaaa tattggaact gattccaggt actaaataaa
     3361 aagaggaata tcattatgtt aagcgtagaa aaagtttatg aaacgtttct gagtcatgac
     3421 gttgattttt ttacaggagt tccagactca ttactaaaaa atatttgtgc ctatatcaca
     3481 gaccatactt cacgtgagaa acatatcata gctgctaatg aaggtgcggc tgtaggaatt
     3541 gcttcaggtt attatatggc atcaggtaag gttcctgtgg tttatatgca aaattccggc
     3601 ttgggtaata ctgttaatcc tctgctgtct cttgcagatg aacaagtata cagttttcca
     3661 atgcttttga tgattggctg gcgtggtgaa ccgggaacaa aagatgaacc tcagcataaa
     3721 aaacagggtg aggtaacttt agatttgttg aaagcaatgc ggattcctta tataattctt
     3781 gattcaaatg agaatgatgc atttattcag ctccatgata tcatacaatc agcaaaaact
     3841 aagaatattc ctcatgcaat aattattcgg aaagatgttt ttggcaaata taaacttcaa
     3901 aaagagttcg gatgtaatta tcctttatct cgtgaggatg ctttgcaaat agttgtggat
     3961 tatctgcctg aaaattcagt ggtcgtttct actactggca agctttctcg tgagttattt
     4021 gaatatcgag aaatgaaaga gcaaggtcat gaacatgatt ttctcacggt aggctctatg
     4081 ggacatagtt catcaatctc tttgggtatt gctatagcaa agcaagatag acctgtttat
     4141 tgcttggatg gtgatggagc atttattatg catttagggg caattagtaa cattggcgat
     4201 ttatctccta aaaactatta tcatattttg tttaacaatg gtgctcacga gtctgtagga
     4261 ggacagccta ctttaggatt tagtttagat attccggcta ttgcccgggg gagcggatat
     4321 aagcatactt atactgtttg tacaaaagtt gaaattgaag aagcaatgaa gcagcttcca
     4381 aagttatgtg gtcctgtttt gttggaaata aaggttaaga tagattctcg ggagaatttg
     4441 ggacgtccca cgactactcc tatagagaat aaggagcatt ttatggattt tttaaaataa
     4501 atatattcga tgaaaataga aagaaaaata ttattaaatc ctggccctgc cactactact
     4561 gacagtgtaa aaatggcaca agttgttccg gatatttgtc ctcgcgagaa agaattcgca
     4621 ggtatgatga aacaacttcg ggacgatttg gtcagagttg ctcatgggaa tttggaaaaa
     4681 catacagctg tattattttg tggctcagga accatcaata ttgatatatg tctaaattct
     4741 cttcttcctg aagacaaaag ggtgcttata gtgaataatg gggcatatag tacccgtgct
     4801 gtagaagtgt gtcaatatta tggtttgcct catattaatt tggaattttc tgtttatgaa
     4861 cgtcctgatt tatcggtagt ggaaaatgtg ttacaagaaa atcctgatat agctatggtt
     4921 tatactactc atcacgagac gggtacagga attctgaacc ctattcgcga gatgggcgat
     4981 atagctcata aatatcaggc taaatttgtc gtcgatacta cttcttcttt gggcatgatt
     5041 ccttttgata tagagaaaga taatgtagat ttttgtatgg catctgctca aaaaggactt
     5101 caagcgatga caggtctttc ttttattata ggcaatgagg aacagattaa ggtttcaaaa
     5161 aaatatccca agcgttctta ctattgtaat ctttatttgc agtatgagaa ttttgagaga
     5221 acaggtgaaa tgcattttac tccaccggta caaactattt acgccactcg tcaggcatta
     5281 aatgaatatt ttgaagtggg cgaagaagct aagtttgccc gtcacaaaag agtttttgag
     5341 gctattcatt caggtataaa tgaaattggt cttaaaagtg taataaagcg tgaatggcag
     5401 tcaggactgg ttgtctcagt tcaataccca gaagatccca actgggattt tgagaaaata
     5461 catgattatt gttatgagcg tggatttacg atttatccag gtaagatatc gactgcgaat
     5521 acttttagaa tctgtgcttt aggagaaata gaagtagatg atattgagaa cttctttaaa
     5581 gtactaaaag ctgcattaaa tcattataac gtcacattac cgatgtcatg attgcagaaa
     5641 tattattgaa gattcgtcat ttttattttt cccttcgtgg tatacacttc catccggaaa
     5701 gtgttatttc tggagtgcct attcgtagct ttcgcttagt aggcaaatat
//
