LOCUS       c00257_gnlPro..         5741 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AIGJNPKI_257.
ACCESSION   c00257_gnlPro..
VERSION     c00257_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 17:08:53
            Original ID  :: gnl|Prokka|AIGJNPKI_257
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5741
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5741
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:5730]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..5730
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..5741
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5741
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             complement(3..794)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg257_1"
                     /sec_met_domain="IlvN (E-value: 7.8e-48, bitscore: 150.6,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 5.5e-22, bitscore: 67.1,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQLNFGGTVENVVIRDEFPLEKAREVLKNETIAVIGYGVQGPGQ
                     ALNLRDNGFNVIVGQRQGKTYDKAVADGWVPGETLFGIEEACEKGTIIMCLLSDAAVMS
                     VWPTIKPYLTAGKALYFSHGFAITWSDRTGVVPPADIDVIMVAPKGSGTSLRTMFLEGR
                     GLNSSYAIYQDATGNAMDRTIALGIGIGSGYLFETTFIREATSDLTGERGSLMGAIQGL
                     LLAQYEVLRENGHTPSEAFNETVEELTQSLMPLFAKNGMDWM"
     CDS             complement(858..1601)
                     /locus_tag="ctg257_2"
                     /transl_table=11
                     /translation="MSEENKIGTYQFVAEPFHVDFNGRLTMGVLGNHLLNCAGFHASDR
                     GFGIATLNEDNYTWVLSRLAIELDEMPYQYEKFSVQTWVENVYRLFTDRNFAVIDKDGK
                     KIGYARSVWAMINLNTRKPADLLALHGGSIVDYICDEPCPIEKPSRIKVTSNQPVATLT
                     AKYSDIDINGHVNSIRYIEHILDLFPIELYQTKRIRRFEMAYVAESYFGDELSFFCDEV
                     SENEFHVEVKKNGSEVVCRSKVIFE"
     CDS             complement(1601..2164)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg257_3"
                     /sec_met_domain="ALS_ss_C (E-value: 2.7e-20, bitscore:
                     60.9, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDKTLYTIIVHSENIAGLLNQVTAVFTRRQINIESLNVSASSIK
                     GVHKYTITAWTDKDTIEKVVKQIEKKIDVIQAHYFTEDEIYFHEIALYKVSTPAFQETP
                     EASKVIRRYNARIVEVNPVFSIVEKNGMSEEITSLYEELRALKCVLQFVRSGRVAITTS
                     CFERVNEFLDGQEAKYKQSKKEQE"
     CDS             complement(2183..3880)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg257_4"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.5e-48, bitscore:
                     152.2, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKDLITGAEAMMRSLEHQGVTTIFGYPGGSIMPTFDALYDHQNT
                     LNHILVRHEQGAAHAAQGYARVSGKVGVCLVTSGPGATNTITGIADAMIDSTPIVVIAG
                     QVGTGFLGTDAFQEVDLVGITQPIAKWSYQIRRAEDVAWAIARAFYIASSGRPGPVVLD
                     FAKNAQVEKTKYEPTQQEFIRSYVPVPDTDEESVKAAAELINNAERPLVLVGQGVELGS
                     AQEELRTFIEKADMPAGCTLLGLSALPTDHPLNKGMLGMHGNLGPNINTNKCDVLIAVG
                     MRFDDRVTGNLATYAKQAKVIHFDIDPAEVNKNVKVDIAVLGDCKKTLAAVTGLLKKNR
                     HTEWVDSFKEYEAVEEEKVIRPELHPATDSLSMGEVVRAVSEATRHEAILVTDVGQNQM
                     ISARYFKYTKERSIVTSGGLGTMGFGLPAAIGATFGRPDRTVCVFMGDGGLQMNIQELG
                     TIMEQKAPVKIICLNNNYLGNVRQWQAMFFNRRYSFTPMLNPDYMKIASAYDIPSKRVF
                     SREELKAAIDEMLSTDGPFLLEACVVEEGNVLPMTPPGGSVNQMLLEC"
     CDS             complement(3928..5730)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg257_5"
                     /sec_met_domain="ILVD_EDD (E-value: 1.9e-218, bitscore:
                     714.6, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKQLRSSFSTQGRRMAGARALWAANGMKKNQMGKPIIAIVNSFT
                     QFVPGHVHLHEIGQLVKAEIEKLGCFAAEFNTIAIDDGIAMGHDGMLYSLPSRDIIADS
                     VEYMVNAHKADAMVCISNCDKITPGMLMAAMRLNIPTVFVSGGPMEAGEWNGQHLDLID
                     AMIKSADDSVSDQEVANIEQNACPTCGCCSGMFTANSMNCLNEAIGLALPGNGTIVATH
                     ENRTKLFEDAAKLIVENAMKYYEEGDESVLPRSIATRQAFLNAMTLDIAMGGSTNTVLH
                     LLAVAHEAGVDFKMDDIDMLSRKTPCLCKVAPNTQKYHIQDVNRAGGIIAILAELAKGG
                     LIDTSVLRVDGMSLAEAIDQYSITSPNVTEKAMSKYSSAAGNRFNLVLGSQGAYYQELD
                     KDRANGCIRDLEHAYSKDGGLAVLKGNIAQDGCVVKTAGVDESIWKFTGPAKVFDSQEA
                     ACEGILGGRVVSGDVVVITHEGPKGGPGMQEMLYPTSYIKSRHLGKECALITDGRFSGG
                     TSGLSIGHVSPEAAAGGNIGKIVDGDIIEIDIPARTINVRLTDEELAARPMTPVTRDRY
                     VPKSLKAYASMVSSADKGAVRLID"
ORIGIN
        1 tacatccagt ccataccgtt ctttgcaaac aacggcatca atgactgagt cagctcttct
       61 acagtttcgt tgaatgcttc ggaaggagtg tgaccgtttt cacgtaacac ttcgtattgt
      121 gccagcaaca gaccctggat agctcccatc aatgaaccac gttcgcctgt caggtcggaa
      181 gtagcttcgc ggatgaaagt tgtttcgaac aaataacctg aaccgatacc gatacccaat
      241 gcgattgttc tgtccatagc gttgcctgtt gcatcctgat agatagcgta agaagagttc
      301 aagccgcgac cttcaaggaa catagtacgc aaggatgtac ccgaaccttt aggagcaacc
      361 atgattacgt cgatatctgc aggaggaact acacctgtgc ggtcactcca tgtaatagca
      421 aaaccatgag agaaataaag agcttttcct gcagtcaggt aaggcttgat agtaggccat
      481 acagacatta ctgctgcatc agacaacagg cacataatga tcgtaccttt ttcgcaagct
      541 tcttcaatac cgaacaaagt ttcacccgga acccatccgt cagcaaccgc tttgtcatat
      601 gtctttccct ggcgttgacc aacgattaca ttgaaaccgt tatcacgaag gttcagagcc
      661 tgtccgggac cttgtacgcc ataaccgatt acagcgattg tttcattttt caatacttca
      721 cgagcttttt ccaatggaaa ttcatcacgg ataactacat tttctacagt tccgccaaaa
      781 ttcaactgtg ccattttttt tgtttttttg ttttgatgac ttgcgccatc tgttattaat
      841 tgattttata ctctatatta ttcaaatatc actttggaac ggcatactac ctcgctgccg
      901 tttttcttca cctctacatg aaactcgttt tcactcacct catcacagaa gaaagagagt
      961 tcatctccga aataactttc agccacatac gccatttcaa accggcggat acgtttcgtc
     1021 tgatacagtt ctatcggaaa caagtcaagt atgtgctcga tataacgaat actgtttacg
     1081 tgtccgttaa tatcgatatc actgtatttt gccgtcagcg tcgcaacagg ctgattgctt
     1141 gtcaccttga tacgcgaagg cttttctatc gggcagggtt catcgcatat ataatcgacg
     1201 atgcttcctc catgcaatgc caacagatcg gcaggcttac gggtattcag attgatcatc
     1261 gcccatacgg aacgggcata gccgatcttc tttccatcct tgtcaatgac ggcaaagtta
     1321 cggtcggtaa agagacgata gacattctcc acccatgtct gaaccgagaa cttctcgtat
     1381 tggtaaggca tctcgtccag ctcgatcgcc agacgggaaa gcacccatgt gtaattgtcc
     1441 tcgttcagcg tagcaatacc aaatccacgg tcgctggcat gaaaacctgc gcaattcagc
     1501 agatgattcc ccagcacgcc catggtcagg cggccgttaa aatctacgtg aaacggctct
     1561 gccacaaact gataagtccc tattttattt tcttcactca ttactcctgt tcctttttac
     1621 tttgcttata cttcgcttcc tgtccgtcca ggaactcatt cacacgttcg aaacagctgg
     1681 tagtaatagc cacacggccc gaacggacaa attgcagcac acatttcaaa gcccgaagtt
     1741 cttcgtaaag ggaagtaatc tcttcactca tgccgttctt ctcgacgata gagaataccg
     1801 gattcacctc tacaatgcgg gcattatatc tgcggatcac tttagatgct tccggagtct
     1861 cctgaaaagc aggagtagat accttatata aggctatctc atggaaataa atctcgtctt
     1921 ccgtaaaata gtgcgcctga atcacatcaa tcttcttttc gatctgcttc accacctttt
     1981 caatagtgtc cttatcagtc cacgctgtga tcgtatattt atgtactcct ttaatggaag
     2041 aagcagaaac gttcaaactc tcgatgttta tttgccgacg ggtaaataca gcggtcacct
     2101 gattcagcaa tccggctata ttttcagaat gaactataat cgtatataat gtcttatcac
     2161 tcataacttt ctaaattaag gattaacatt ccagcaacat ctgattgact gaaccgcccg
     2221 gaggggtcat cggcaggaca ttgccttctt ctaccacaca ggcttcgagc aggaacggcc
     2281 cgtctgtcga gagcatttcg tcgatggccg ctttcagttc ttcccgtgaa aagacacgtt
     2341 tggaaggaat atcgtaggcg gaagcgattt tcatgtaatc cggattcagc atcggagtaa
     2401 atgaataacg gcgattaaag aacatggcct gccattggcg cacgttgccg agataattgt
     2461 tgttcaggca gataatcttc accggagctt tctgttccat gatcgtaccc agctcctgaa
     2521 tgttcatttg cagtccgccg tcacccataa atacgcatac cgtgcggtcg ggacgtccga
     2581 aagttgctcc gatagctgcc ggaaggccaa aacccatcgt gccgagtccg cccgaagtta
     2641 cgatactacg ttccttggta tatttaaagt agcgggcaga gatcatctga ttctgaccta
     2701 catctgtcac caatatggct tcgtgacggg tcgcttcgct taccgcacgc accacttcgc
     2761 ccatactcag tgaatccgtt gcaggatgaa gttcgggacg aatgactttt tcttcctcta
     2821 ccgcttcata ttccttgaaa ctatcgaccc actcggtgtg ccggttcttt ttcagcagtc
     2881 cggtcacagc agccagcgtc tttttgcagt cgcccaaaac ggcaatgtct acctttacat
     2941 tcttatttac ttccgccgga tcaatgtcaa aatggatcac tttcgcttgt ttggcgtaag
     3001 tagccagatt accggtcaca cggtcgtcga aacgcatacc gacagcaatc agtacatcac
     3061 atttgttagt attgatattc ggtcccagat ttccgtgcat acccagcatt cctttattca
     3121 acggatggtc ggtaggcaat gcggaaagtc cgagcaaggt acaacctgcg ggcatatccg
     3181 ccttttcgat aaaggtacgc agttcttcct gagcgcttcc cagttccact ccctgtccta
     3241 ccaagacaag cgggcgttcg gcgttattga tcagttcggc ggcagccttt accgattctt
     3301 cgtctgtgtc gggaaccgga acatagctgc ggataaactc ctgctgtgtc ggctcgtatt
     3361 ttgttttttc aacctgcgca ttcttggcaa agtcgagtac caccggaccg ggacggccac
     3421 tactggcaat gtagaaagca cgtgcgatcg cccaggctac gtcttcggca cggcgaatct
     3481 gatagctcca tttagcgatt ggctgagtga tacctacgag atcgacttcc tgaaaagcgt
     3541 ctgttccaag aaagcctgtt cccacctgtc ctgcaatgac aacgataggc gtactgtcta
     3601 tcattgcatc tgcaatacct gtgatcgtat tggttgctcc agggccgctt gtcaccaagc
     3661 aaacaccgac tttacccgat acacgggcgt agccttgtgc cgcatgggca gctccctgtt
     3721 catggcgaac caagatatgg ttcaatgtat tttgatggtc gtacagggca tcgaacgtag
     3781 gcatgatgga accgccggga taaccgaaaa tggtagtcac tccctgatgt tccagagagc
     3841 gcatcattgc ctctgcgcct gttattaagt ccttactcat agttctttaa tggataatgg
     3901 atgattcata atggataatg aagtggatca gtctattaat ctcactgctc ccttatcggc
     3961 agagcttacc atgctggcgt atgctttcag gctcttcggc acgtaacggt cacgggtgac
     4021 gggagtcatc gggcgggcag ccagttcttc atctgttagt cgcacgttaa tcgttcgggc
     4081 gggaatgtcg atttcgatga tatctccatc tactattttt cctatattac ctccggcagc
     4141 ggcttcggga gaaacgtgtc cgatactcaa tccggaagtt ccaccactga aacgtccgtc
     4201 ggtaatcaag gcacattctt tgccgaggtg acgggatttg atataagaag tcgggtaaag
     4261 catttcctgc ataccgggac cgcccttcgg accttcgtgc gtgatgacga caacgtcgcc
     4321 gctgacgact cggcctccga ggatgccttc acaggctgct tcttgtgagt cgaatacttt
     4381 ggcaggtccg gtaaacttcc agatgctttc gtctacaccc gccgttttta ctacgcaacc
     4441 gtcctgagcg atatttcctt tcagtaccgc caggcctccg tccttgctat aggcgtgctc
     4501 cagatcacgg atacatccgt tcgcgcgatc tttatctaat tcctgataat aagctccctg
     4561 agaaccaagt acgagattga atctgtttcc tgccgcactt gaatatttgc tcattgcttt
     4621 ttccgtaacg ttcgggctgg taattgaata ttgatcgatc gcttccgcca gcgacatgcc
     4681 atccacacgg agaacggaag tatcaatcag accgcctttt gccaactctg ccaggatagc
     4741 aatgatgcca cccgcacggt taacgtcctg aatatgatat ttttgagtat tcggagcgac
     4801 tttacacagg cagggtgtct tgcgtgacag catatcgatg tcgtccatct tgaaatcgac
     4861 tcctgcctca tgtgccacgg ccaacaggtg aagcaccgta ttggtagaac cgcccatcgc
     4921 aatatccagc gtcatggcat tgaggaatgc ctgacgggta gctatgctgc ggggaagtac
     4981 actttcgtca ccttcttcat aatatttcat tgcattttcc acaatcagtt tggcagcatc
     5041 ttcaaacagt ttcgtacgat tttcgtgcgt agccacgatt gtgccgtttc ccggaagagc
     5101 caagccgata gcttcattca gacagttcat cgagtttgcc gtaaacattc cggaacaaca
     5161 tccgcaggta ggacaggcat tctgttcgat attcgctact tcctgatcgc ttacactgtc
     5221 gtccgccgat ttaatcatgg catcgatcaa gtccagatgc tgaccgttcc actctccggc
     5281 ttccatcggt ccgccggata caaatacggt agggatattc aggcgcatgg cagccatcag
     5341 catccctggg gtaatcttat cgcagttgct gatacatacc attgcatccg ccttgtgcgc
     5401 attgaccata tattctacgc tgtccgctat gatatcacgg gaaggaagcg agtaaagcat
     5461 tccgtcatgt cccatagcga taccgtcatc aatagcaatc gtattaaatt ccgcagcgaa
     5521 gcatcccagc ttctcaattt ccgccttcac caactgcccg atttcatgca ggtgcacgtg
     5581 tcccggaaca aattgtgtaa acgaattgac gatagcgata atgggttttc ccatctgatt
     5641 tttcttcatg ccgttggctg cccataatgc gcgggctccc gccatgcgac ggccttgtgt
     5701 actaaacgaa ctgcgtaatt gctttttcat ttcaatctat c
//
