LOCUS       c00074_gnlPro..         7437 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PJKALHPE_74.
ACCESSION   c00074_gnlPro..
VERSION     c00074_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 17:20:18
            Original ID  :: gnl|Prokka|PJKALHPE_74
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7437
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7437
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[4182:7153]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4183..7153
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7437
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7437
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             24..329
                     /locus_tag="ctg74_1"
                     /transl_table=11
                     /translation="MEAKTNEEFFNVSALIDERFGKEGTASRAEAEEKAYAFYTGQIIE
                     DARKKAKITQAELARRIGSDRSYISRVESGQTEPKVSTFYRIMNALGCKIEFSMIL"
     CDS             complement(399..2513)
                     /locus_tag="ctg74_2"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYVIAEMTEEEIVSTISSFTYREKVEEIRRIFAEQ
                     GEKAANEKKKELPAIAFSASYRGRRTKVNLVKYMGHIVIDIDHLSKEELARILPVIKRC
                     NYTRIAFISPKGMGVKIIVRACHPDETLPETLQEIEDFHHAAYTRLVSFYTELCQIEID
                     TSGQDVARTCLFSYDPEIYFNPNADAFLVDQPQASYKVSNRKNLFGSKQQTPPDGTPTN
                     EDTALNAHSANASLVLTLTYYHNKSEKYIAGNRNNYLHHLSCTFNRYGIPQEEASAFIK
                     SQFTDFPADETVSLINSAYAHTDEFNTCKLNGTQKRILRIEQYISEHYETRYNEVLHIM
                     EYRRRRPDTEKPEPFRILDEMMENSIWIEINELGYPCTVKTIENLIYSDFSQSYHPIRE
                     YFELLPEWDGTDYIRILADSVQTNHQEFWAECLERYLVAMCAAATQENIVNHTVLLLCS
                     EIQNIGKTTFINNLLPPELRAYLSTGLINSNNKDDLAKITQAMLINLDEFEGMSGRELN
                     AFKDLVTRKVISFRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHPYSIQF
                     ITINYAQLYAQIKYLLNKPGYQYWFTQADNERLEENNEAFIFHSPEEEMVLTHIRKPER
                     FEKVYYLTVSEIAELIRERTGYQYSIGSKIQLGKVMTKHHFESKKGNNGRRYAVFIIDI
                     EQVKSNRLYE"
     CDS             complement(2600..2830)
                     /locus_tag="ctg74_3"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             3024..3527
                     /locus_tag="ctg74_4"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLIFRPGKTLAELMKILSYQQVLTRAVAQAGAGDDGGEDPDKNPGSGGDGSGDEEAP
                     DPTV"
     CDS             3587..3754
                     /locus_tag="ctg74_5"
                     /transl_table=11
                     /translation="MKEIVNRILDVIMYLIPFFGKRKRDKVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             3741..4190
                     /locus_tag="ctg74_6"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(4183..4911)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 71.7;
                     E-value: 2.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg74_7"
                     /sec_met_domain="Radical_SAM (E-value: 1.8e-29, bitscore:
                     92.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKTLVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(4925..7153)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg74_8"
                     /sec_met_domain="Gly_radical (E-value: 5e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1174.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLRRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     KFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 aaagatatta agaaaggagt attatggaag caaaaacaaa tgaggaattc tttaatgtca
       61 gtgcattaat agatgagcgt tttggtaagg aaggaacagc ttctcgtgca gaggcagaag
      121 aaaaggccta cgccttttat acagggcaga ttatcgagga cgcaagaaaa aaggctaaaa
      181 tcactcaggc tgaattagcc cggcgtatcg gttctgaccg ttcttatatt tcaagagtag
      241 aaagtggtca gacagagcct aaagtatcta ctttctatcg tataatgaat gcgttaggct
      301 gtaaaatcga attttcaatg attttgtaaa aacaataaat gcagagtatc tgtcaagatg
      361 actctgcatt ttgctatatt cacaaggctt tcccgcttct attcatacag cctgtttgat
      421 tttacctgct cgatatctat aataaatacg gcatatcggc gcccgttatt tcctttttta
      481 gattcaaaat ggtgcttagt catcaccttc cccaactgta tttttgatcc gatggaatac
      541 tgataaccgg tacgctcacg tattagttcc gcaatctcac tgacagtcag ataataaacc
      601 ttctcgaaac gttccggctt gcggatatgc gtaagaacca tttcctcttc cggtgaatga
      661 aagatgaaag cctcgttatt ctcttcaagc cgttcattgt ccgcttgcgt gaaccagtat
      721 tgatatcccg gcttattcag cagatatttg atctgagcat agagctgcgc ataattgatg
      781 gtaataaatt ggatggaata aggatgaaaa caaaggaaac gccggttgcc tgtcgtgtcg
      841 tgaagcactt cctgatagtt gcaggttcct gcaaaagagg ccgtatgagg gaagttttgc
      901 gaacggcgtg cataaggcag acggaaactg atcactttgc gcgttaccaa atctttgaat
      961 gcattcagtt cccgaccgct catcccttca aactcgtcga gattgatcag catggcctgc
     1021 gttattttgg ctaaatcatc tttattgttc gaattgatta gtccggtaga aagataagca
     1081 cgcagttcgg gaggcaggag attattgata aaggtagtct ttcctatgtt ctgtatctcg
     1141 ctgcatagca gaagtaccgt gtgattgaca atattctcct gagtagccgc tgcacacatc
     1201 gccaccagat aacgttccag gcattccgcc cagaactcct gatgattcgt ctggacacta
     1261 tccgccagga tacggatata gtcggttccg tcccattcgg gcagtaattc gaaatactcg
     1321 cgaatgggat gatacgactg gctgaaatcg gagtaaatca gattttcgat tgttttgaca
     1381 gtgcagggat atcccagctc gttgatttct atccagatac tgttttccat catctcatcc
     1441 aagatgcgga aaggctccgg tttctcggtg tcgggacgcc ttctgcgata ttccatgata
     1501 tgcagcactt cgttgtagcg tgtttcgtag tgctcgctga tatattgctc tatccgtaat
     1561 attcgtttct gcgtgccatt cagcttacag gtattaaatt cgtcggtatg agcgtaggcg
     1621 ctattgatca aagagactgt ctcgtctgcc ggaaagtctg taaactggct tttgataaag
     1681 gcggatgctt cctcctgcgg aatgccgtag cgattgaagg tacatgagag atggtgcaga
     1741 taattgttcc ggttgcctgc tatgtatttt tcggacttgt tgtgatagta ggttaaagtc
     1801 agcaccagag aagcgttggc ggaatgggcg ttgagcgctg tgtcttcatt cgtcggggtg
     1861 ccgtccggag gagtctgttg tttactgccg aagagatttt tacgatttga aaccttgtag
     1921 gatgcctgag gttgatctac aagaaaagcg tcagcgttcg gattgaaata aatctccgga
     1981 tcgtaagaga acagacaagt gcgtgccaca tcctggccgg aggtgtcgat ttctatctga
     2041 cagagttcgg tgtaaaaaga aacgagtctg gtgtaagctg catgatggaa gtcttctatt
     2101 tcctgaaggg tttcgggcaa tgtctcatcc ggatggcatg cacgcactat gattttcacg
     2161 cccatccctt tgggactgat aaaagcgata cgggtgtaat tgcatctctt gatgacaggg
     2221 aggatgcgcg ccaattcttc tttgctgaga tggtcgatgt ctatcacgat gtgtcccata
     2281 tatttgacga gattcacttt ggtacgcctc cctcggtagc tggcggagaa ggcgattgcc
     2341 ggcaattctt ttttcttctc attggcagcc ttttctcctt gctctgcaaa gatgcggcgt
     2401 atttcttcga ctttttcccg gtaggtaaaa ctgctgatag tggacactat ttcttcttcg
     2461 gtcatctctg ctattacgta gtagaaacct tgaaatatgg tgaaatgttt cattttgatt
     2521 ttaattgttt tttgtcagtg caaaagaact tctttgcgga aatgtgtaaa ggaactttgt
     2581 atagaatggc aataagttac tacggttcgc ccaaaaagtg tatgatagtt gccacttgtt
     2641 tcggcgaaag gaggcggttg cgtgcgcggt aaccttcctg ttcgagttcg tgcaggagag
     2701 ggaggttgta aataatccaa cgacgaagta tttgtaaagc aactttgagg cagacatggg
     2761 gattgtaaag gtgagccagt tccgctttgg tataggtgcg gataaggaaa gaatcttctt
     2821 tgtaattcat gatgttttct tcttgtttta tgctataaag ttacataaaa tttattgttt
     2881 ttcaaaggtt tgacttcgtt tttattcgtt ggaaatcaag aaacttcgtt ggggaatatg
     2941 gcttgtccgg atacccttat ctttgtgctg ttaacaagaa agggatgcat cctacgtatt
     3001 ataaacttta aaattatttt attatggcaa tgacagtatc ttattcagtg gtgccgcgca
     3061 agaatcctgc aaaaaagagc gaaccggcaa agtattatgc acaggcgcag gcgtcggggg
     3121 agttggactt tgaagagttg tgcgaagcga tcaccagtcg ttctacttgt acggaaacgg
     3181 atgtacgtgc ggctatttcg ggtattcttt acgaagtgaa gcgtgcgttg aaggcaggaa
     3241 gaattgcgag actgggcgat ttgggcagtt tgcagatagg gctgaacagt gaaggtgcgg
     3301 catcggtcaa ggagttttcc ggttcgatga ttaagggggc gcatcttatt ttccgccccg
     3361 gtaagacgct ggcggagttg atgaagattc tgagttatca gcaagtgctg actcgtgcgg
     3421 tggcgcaggc gggtgcgggc gatgacgggg gagaagaccc tgacaagaac cccggttccg
     3481 gcggtgatgg ctccggcgat gaagaggctc cggaccctac ggtataattg gcagacgggt
     3541 caaggtaata acgaattaga ataacttcca ttaatatttt gataaaatga aagaaatagt
     3601 aaaccggatt ttagatgtga tcatgtatct gattcctttc ttcggaaaga gaaagcggga
     3661 caaggtggtg agagaagtgc gctatcatac tacttgtaag gaggtgtgca aagtgaaaac
     3721 gacggaaagg gaaaaggaac atgagaaaga ttagtctgat cgtgatccat tgttccgcca
     3781 cccgtgtcga ttgcgacttt acggcaaagg atgtggatac ggctcaccgt tatcgtggct
     3841 tttcgtgctg gggctatcat tattatatac gcaagtcggg cgagattgag ccgatgcggg
     3901 atgaagatac ggtcggggct catgcacgtg gctacaatgc gatcagttta ggtgtatgct
     3961 atgagggcgg tttggatgag aatggaaaag cggcggatac gcgtacaccc cgtcagaagg
     4021 aggcgttaca tcgtttggta cacgaattgt tgcagcgtta tccggaagct aaggtagtag
     4081 gtcatcggga tttaagtccc gacaccaact ataatgggat cgtagatcct tgggaacgga
     4141 ttaaagaatg cccttgcttt gaggtcattg gagaattctc cattaattaa ctaccaccgt
     4201 agtgaaatac tctttgaata cctccgcagc tttttcaagc tgttcggggg tattttcttt
     4261 tacccctttc atcttgtatt cctgtcccat cgcttcgtac ttatgtacgc ccagtgtatg
     4321 gtaaggcagt atttccaccc gttggatcat cttgtatttt ccgagtgctt ctcccagtgc
     4381 gcgtatgtct tcttcaaaat cgctgtatcc gggcactagc acgtagcgta gccagaaagg
     4441 tttctcctgt tcttccagcc aggcggccgt gcggatggtt tgctcgttgc ttcttccggt
     4501 gagcgtctga tggcggttgg ggttgaattc ctttatatcc aataacacaa gatcggtcag
     4561 tctgaataat tcttctacgt cttcgttcca aagtcctccg ttgctgtcca gacagacatg
     4621 gatgcctttt tctttcagtt cgcggaccag cgggacgagt gttttcgcct gaaacgtcgg
     4681 ttcgcctccg gagaaagtga ttcctcctcg tttcccgaag aaggggcgct ggctcattgc
     4741 catgcggacg atttcttccg gtggggtagg cgtgcctccc tttcctgcta tcgtatccgg
     4801 attggcgcaa tagaggcagc ggaagttgca tccttgaaga aaaacgacga gccgtaagcc
     4861 cggcccgtcg aatgttccca tactttcgta tgaatgtacg tttatcatca tatagagtaa
     4921 tagattacat acgttcgtgg aagctacggc tgataacttc cagttggtgt tcgcggctca
     4981 gcttcacgaa gtttacggca tagccggaaa cgcggatagt cagctgcgga tagttttccg
     5041 gatgctccat ggcatcgtac agcatatcac ggttcagtac gtttacgttc aggtggtgag
     5101 cacctttggt gaagtaaccg tccatcatcg ttacgaggtt ctcgatacgg tcttcgtccg
     5161 tagcacccag tgatttcgga acgatagaga aggtgttgct gataccgtcc tgtgagtcac
     5221 ggtaacgaag tttcgctaca gagctcagag aagcgatcgc accattcttg tcgcgtccgt
     5281 gcatcgggtt ggcacccgga gcgaaggcaa cacctttggc acgtccgtcg ggagtagcac
     5341 cggtcttttt gccgtacatc acattggaag tgatagtcag caaggagagg gtaggacggg
     5401 cattcttgta aactggcaat ttcttcaatt cttcgctgaa gaagtatacc aggtctacgc
     5461 caaggtggtc taccttgtcg ttgtcgttac cgaagcaagg gaattctcct tcgatgtcga
     5521 aaccttctgt caggccgata tcgttgcggc gggcagttac tttcgcatat ttgatggcag
     5581 acagtgagtc gagtgcgatg gaaagtccgg caacaccgta agcaaggttt atgcgcgggt
     5641 tcgtatctac aagagccatc tgggctttct cgtagtaata cttgtcgtgc atatagtgga
     5701 tgatgttcat cgcttcgttg tatacgcggg cgatttcggt caacactttc ttgtagttgt
     5761 ccattacttc ttcgaacttc agcgtgtcac tggtcagtac ggggatgttc ttcaccatga
     5821 ctgtaccggt attctcgcaa cgtccgccgt tgatggcaag caacagggct ttggccaggt
     5881 tgcaacgggc accgaagaac tgaatctgct tgcctatttc ctggtatgat acgcagcagg
     5941 cgattccgta gtcgtcggac tgacgtactt cacgcatcag gtcgtcgttt tcgtactgga
     6001 tagaagaagt gtcgatagaa acctttgcac agaattcttt gaatccttcg ggaagttccg
     6061 ggctccagag tacggtcagg ttcggttccg gtgaagggcc gaggttgtac aatgtctgca
     6121 ggaaacggaa ggaggtcttt gtcactttgg tacgtccgtc gttgagacgt ccgcccagag
     6181 attcggttac ccaggtcggg tcgcctgcaa agatgtcgtt gtatgattgc atacgcaggt
     6241 ggcggaccat acgcagtttg atgacaaact ggtcgatcag ctcttgcgcg aacgattcgg
     6301 tgatggttcc tttgctcagt tcatattcca gatagatatc gaggaaagaa gaaacgttac
     6361 ccagtgacat ggcggcaccg tcttgttctt tgacggcggc aaggtaagcc atatataccc
     6421 actgtacggc ttcttgtgcc gtgtaagcgg gacggctcag gtcgagaccg tagtattcgc
     6481 ccatcacttt catgtctttc agtgccttga tctgttctgc cacttcttcg cgcagacgga
     6541 tacgggcctc tgtcatcgga ccggtgaggt tatgcaaatc ttccttcttt gcttcgatca
     6601 gacggtcgat gccgtaaaga gccatacggc ggtagtcacc gatgatacgt ccgcgtgcat
     6661 aattgtcggg aagtccggtg aggaatccca gcgaacggaa ggaacggatt tcttccgtat
     6721 acacgtcgaa tactccgtcg ttgtgcgtct tgcggtagtg agtgaaaata tcttttacgc
     6781 ggtcgtccac ttccacaccg ttttcgtgac aggctttgct gactacgttg atacctccga
     6841 aaggcttgat ggcacgtctc agaagttcgt ctgtctgcag gccgacgatc agttcgtttt
     6901 ccttgtcgat atatccggcc ttgtgggaag tgatggttga tacggtaaca ttatctaatg
     6961 agcggacacc gttgttttct ctttcttctg ccaacgcttc gaggcagcgg ttccatacgg
     7021 ctttggtacg ttctgtaggt ccttcgagga atgaagcatc tccgtaatac ggagtgatgt
     7081 tatgacttac gaaatctctg acgttgattt cgctgctcca aagaccgtct ttaaagatct
     7141 tgtttaattc cataaaatga taaaattaga ggttgtagtt atcctattct ttagagcgca
     7201 caaagttaca atcatttttc atataatcgg cataaatatg ctctatttta tgcttttcaa
     7261 cataaaatac ctattaattg ctatccttag gaaaagatta gattaaattt ttagtgaaaa
     7321 ctcttgcggg tttaaaatat ctttgtacct ttgcaccgct tttaacgaaa agcacttctg
     7381 ataaggaagt tttggagagg tggcagagtg gtcgattgcg gcggtcttga aaaccgt
//
