LOCUS       c00330_gnlPro..         2494 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GJDAMLMO_330.
ACCESSION   c00330_gnlPro..
VERSION     c00330_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 18:14:38
            Original ID  :: gnl|Prokka|GJDAMLMO_330
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2494
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..702)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.5;
                     E-value: 1.4e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg330_1"
                     /sec_met_domain="Radical_SAM (E-value: 2.5e-30, bitscore:
                     93.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNHHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKE"
     protocluster    1..2494
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2493]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2493
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2494
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2494
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(716..2023)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg330_2"
                     /sec_met_domain="Gly_radical (E-value: 7.9e-48, bitscore:
                     149.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 7.3e-184, bitscore:
                     600.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKTSFRFLQTLYNLG
                     PSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQSDDYGIACCVSYQ
                     EIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNYKKV
                     LIEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKY
                     AKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNARPT
                     LSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDS
                     QDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPE
                     NYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             complement(1978..2493)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg330_3"
                     /sec_met_domain="PFL_acetate1 (E-value: 3.2e-93, bitscore:
                     300.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GFLTGLPDNYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPM
                     TEARIRLREEVAEQIKALKDMKVMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDG
                     AAMSLGNVSSFLDIYLEYELSKGTITESFAQELIDQFVIKLRMVRHLRMXXXXCNLTTT
                     SLPATRHG"
ORIGIN
        1 ctctttaaat acctccgcag ctttttcaag ttgttcgggg gtattttctt ttaccccttt
       61 catcttgtat tcctgtccca tcgcttcgta cttatgtacg cccagtgtat ggtaaggcag
      121 tatttccacc cgttggatca tcttgtattt tccgagtgct tctcccagtg ctcgtatgtc
      181 ttcttcaaaa tcgctgtatc cgggcactag cacataacgc agccagaaag gtttctcctg
      241 ttcttccagc caggcggccg tgcggatggt ttgctcgttg cttcttccgg tcagcgtctg
      301 atggtggttg gggttgaatt cctttatatc caataacaca agatcggtca gtctgaataa
      361 ttcttctacg tcttcgttcc aaagtcctcc gttgctgtcc agacagacat ggatgccttt
      421 ttctttcagt tcgcggacca gcgggacgag tgctttcgcc tgaaacgtcg gttcgcctcc
      481 ggagaaagtg attcctcctc gtttcccgaa gaaggggcgc tggctcattg ccatgcggac
      541 gatttcttcc ggtggggtag gcgtgcctcc ctttcctgct atcgtatccg gattggcgca
      601 atagaggcag cggaagttgc atccttgaag aaaaacgacg agccgtaagc ccggcccgtc
      661 gaatgttccc atactttcgt atgaatgtac gtttatcatc atatagagta atagattaca
      721 tacgttcgtg aaagctacgg ctgataactt ccagttggtg ttcgcggctc aacttcacga
      781 agtttacggc atagccggaa acgcggatgg tcagctgcgg atagttctcc ggatgctcca
      841 tggcatcgta tagcatatca cggttcagta cgtttacgtt caggtggtgg gcacctttgg
      901 tgaagtaacc gtccatcatc gttacgaggt tctcgatacg gtcttcgtcc gtagcaccca
      961 gtgatttcgg aacgatagag aaggtgttgc tgataccgtc ctgtgagtca cggtaacgaa
     1021 gtttcgctac agagctcaga gaagcgatcg caccattctt gtcgcgtccg tgcatcgggt
     1081 tggcacccgg agcgaaggca acacctttgg cacgtccgtc gggagtagca ccggtctttt
     1141 tgccgtacat cacattggaa gtgatagtca gcaaggagag ggtaggacgg gcattcttgt
     1201 aaacaggcaa tttcttcaat tcttcgctga agaagtatac caggtctaca ccgaggtggt
     1261 ctaccttgtc gttgtcatta ccgaagcaag ggaattctcc ttcgatgtcg aaaccttctg
     1321 tcaggccgat atcgttgcga cgggcagtta ctttcgcata tttgatggca gacagtgagt
     1381 cgagtgcgat ggaaagtccg gctacaccgt aagcaaggtt tatgcgcggg ttggtatcta
     1441 caagagccat ctgggctttc tcgtaataat acttgtcgtg catatagtgg atgatgttca
     1501 tcgcttcgtt gtatacgcgg gcgatttcga tcagtacttt cttgtagttg tccattactt
     1561 cttcgaactt cagcgtgtca ctggtcagta cggggatgtt cttcaccatg actgtaccgg
     1621 tattctcgca acgtccgccg ttgatggcaa gcaacagggc tttggccagg ttgcaacggg
     1681 caccgaagaa ctgaatctgc ttgcctattt cctggtatga tacgcagcag gcgattccgt
     1741 agtcgtcgga ctgacgtact tcacgcatca ggtcgtcgtt ttcgtactgg atagaagaag
     1801 tgtcgataga aacctttgca cagaattctt tgaatccttc gggaagttcc gggctccaga
     1861 gtacggttag gttcggttcc ggtgaagggc cgaggttgta caatgtctgc aggaaacgga
     1921 aggaggtctt tgtcactttg gtacgtccgt cgttgaggcg tccgcccaga gattcggtta
     1981 cccatgtcgg gtcgccggca aagatgtcgt tgtaagattg catannnnnn nnnncatacg
     2041 caggtggcgc accatacgca gtttgatgac aaactggtcg atcagctctt gcgcgaacga
     2101 ttcggtgatg gttcctttgc tcagttcata ttccagatag atatcgagga aagaagaaac
     2161 gttacccagt gacatggcgg caccgtcttg ttctttgacg gcggcaaggt aagccatata
     2221 tacccactgt acggcttctt gtgccgtgta agcgggacgg ctcaagtcga gaccgtagta
     2281 ttcgcccatc actttcatgt ctttcagtgc cttgatctgt tctgccactt cttcgcgcag
     2341 acggatgcgg gcctctgtca tcggaccggt gaggttgtgc aaatcttcct tctttgcttc
     2401 gatcagacgg tcgatgccgt aaagagccat acggcggtag tcaccgatga tacgtccgcg
     2461 tgcatagttg tcgggaagtc cggtaaggaa tccc
//
