LOCUS       c00078_gnlPro..         5420 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GLHNCKEN_78.
ACCESSION   c00078_gnlPro..
VERSION     c00078_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 17:50:36
            Original ID  :: gnl|Prokka|GLHNCKEN_78
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5420
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5420
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2158:5117]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2159..5117
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5420
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5420
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             64..612
                     /locus_tag="ctg78_1"
                     /transl_table=11
                     /translation="MNELELSERCRQGDNRARKELYEQYAGRMLGVCLRYAGDRDMAQD
                     LVHDGFLKIFDSFDKFTWRGEGSLRAWMERVMVNTALQFLRKNDVMNQTTALDEVPETY
                     EEPDASAVEAIPQKVLMQFINELPAGYRTVFNLYTFEDKSHKEIAQMLGINEKSSASQL
                     FRAKSVLAKKVKEWLVTNG"
     CDS             667..1926
                     /locus_tag="ctg78_2"
                     /transl_table=11
                     /translation="MKEDEKWIKAFKDKLEDYSEPMPASGWERLERELMPVTEKRIYPY
                     RRWAVAAAAVVLVLTTAVSLYFLNSPVADEIRYATAPSLAVNPDVLPEPALPDVQVAVS
                     EPVKPVGTTSINPVSGYLAKNTDPVIVPEVSSLVEKRPEAVTEEKRSEPQQEAIAAIEK
                     KESATAQPPKRKEARRPSGKDKYQLPIGDSSAKRGGKWSMGVGIGNGGGLPTNGSENFA
                     PRPMTNRVDLMTIMNGAVSIPADQEVIFEEGVPYLKSNTTAVVDYEHHQPVSFGLSVRK
                     SLPKGFSVETGLTYTLLSSDIKRQGDTKMQSQKLHYIGIPVRGNWNFLEKKYFTLYVSA
                     GGMVEKCVYGKLADDKVNVKPLQFSVAGAVGAQFNATDHVGLYVEPGVSYFFDDGSKVQ
                     TIRKERPCNFNLQAGLRFTY"
     CDS             complement(2159..2884)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.3;
                     E-value: 1.6e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg78_3"
                     /sec_met_domain="Radical_SAM (E-value: 2.4e-31, bitscore:
                     97.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIEAKGG
                     TATDPEEIVRMAVSQKAFFGKKGGITFSGGEPTFQAKSLIPLFKRLKEAGIHICLDTNG
                     GLWNNDVEELLELTDLVLLDIKEFNPEHHQSLTGRSNEQTLKTAAWLETNHKPFWLRYV
                     LVPGYSDFEDDIRQLGEHLGTYQMIQRVEILPYHTLGVHKYEAMNKEYMLKGVKENTPE
                     QIEKAEKLFRQYFRTVQVN"
     CDS             complement(2889..5117)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg78_4"
                     /sec_met_domain="Gly_radical (E-value: 1.8e-47, bitscore:
                     148.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1167.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFKDGLWSKEINVRDFVSNNITPYEGDASFLQGPTERTKA
                     VWNHCLKALEEERNNNGIRALDYTTVSTITSHPAGYIDKENELIVGLQTDQVLKRAIKP
                     FGGINVVMKACRENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGLDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKEIK
                     VLGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSQ
                     GTITETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKAFCAQVSIDTSSVQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKDIPVLTGETL
                     KFEEVMANYKKVLIQIARVYNEAMNIIHYMHDKYYYEKAQMAFVDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAHVKARRNDIGLTEGFDIEGAFPCFGNDDDRVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGIAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATQEDRIDNLVTMMDGYFTKGAHHLNVNVLN
                     REMLQDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             5207..5320
                     /locus_tag="ctg78_5"
                     /transl_table=11
                     /translation="MLNSIKIPIYGEKPEGGYYFQIYFSNIFLRNNLQVKK"
ORIGIN
        1 tgttctctct gtgcagtatt tctatctttg ctgcatttat aaggtaaaca cgtgtttagg
       61 agaatgaatg aattagagct gtcggaacgt tgcaggcagg gagataatcg tgcccgcaaa
      121 gaactttatg agcagtacgc agggcgcatg cttggtgtct gtcttcgtta tgccggagac
      181 agggatatgg cacaagactt ggtacacgac ggtttcctga agattttcga ctccttcgac
      241 aagtttacct ggcggggtga aggctctctg agagcatgga tggagcgtgt aatggtgaat
      301 acggctttac agttcttaag aaagaacgac gtgatgaacc agaccacggc actggatgaa
      361 gttcccgaaa cgtatgaaga accggatgct tcggctgttg aagcaatacc acagaaagtg
      421 ctgatgcaat ttattaatga actccccgcc ggatatcgta ccgtattcaa tttatacacc
      481 tttgaagata agtcgcacaa ggaaatcgca cagatgttgg ggattaatga aaaatcttct
      541 gcctcacagc tttttcgcgc aaaaagtgta ttggcaaaga aagtgaaaga atggttggtg
      601 accaatggtt gacatgtatt tacagatact gaggtgaacc ttggatttca aaaatgaaat
      661 agaatgatga aagaagacga aaaatggatt aaagcattca aggataagct cgaagattat
      721 tcggagccga tgcctgcttc cggttgggaa cgactggaaa gggaactgat gcctgtaacc
      781 gaaaaacgaa tatatccgta tcgccggtgg gcggttgctg cggcagctgt ggtattggtg
      841 ctaacgactg ctgtcagttt gtattttctg aatagtccgg tagctgatga aatccgttat
      901 gcaacggctc cttcactggc tgtcaacccg gatgtattgc cggaacctgc cttgccggat
      961 gtacaggtag ctgtatccga accagttaag ccggtaggaa cgacgagtat taaccctgtt
     1021 tcgggctatt tggcaaagaa tactgatccg gtgattgttc cggaggtatc ctccctggta
     1081 gagaagcgac cggaggcagt gacggaggaa aagagatctg agccgcaaca ggaagcaatt
     1141 gctgcaatcg agaaaaaaga aagtgcgacg gcgcagcctc caaagaggaa agaagcgcgg
     1201 cgaccgtccg gtaaagacaa gtatcagttg cctatcggag actcttctgc taaacgtggt
     1261 ggtaaatggt cgatgggagt gggtatcggt aatggaggtg gacttccgac aaatggttcc
     1321 gaaaattttg cgccccgtcc gatgactaac agggtcgact tgatgacgat catgaatgga
     1381 gctgtgagta ttcctgccga tcaggaagtt atatttgagg aaggggttcc ttacctgaag
     1441 tccaatacaa ctgccgtggt ggattatgaa catcatcagc cggttagctt cggactgtct
     1501 gtacgtaagt ctcttcccaa aggtttctct gtagagacgg gattgactta tacgttgttg
     1561 tcttctgata tcaaacggca gggggatacc aagatgcaga gtcagaaatt gcattatata
     1621 ggtattcctg tacgtggaaa ctggaatttt ctggaaaaga aatattttac actctatgta
     1681 tctgccggag gaatggttga aaagtgtgtt tatggaaagt tggcggatga taaggtgaat
     1741 gtgaagcctt tgcaattttc tgtagccggt gccgtgggag cgcagttcaa tgcgacagat
     1801 catgtcggcc tatatgtgga accgggagtt tcgtatttct ttgatgatgg ctccaaagta
     1861 caaaccatcc gaaaagaaag gccttgtaac tttaacttgc aggcaggatt gcgattcact
     1921 tattaaagta gggtggcaaa aaatatgccg gttacattat ctccctgttc ttgccttgta
     1981 ttgtaatgaa ttaaggcaag aacagggaga cttttatatc gtgacttaca aaattataaa
     2041 taagtgcagt cgtcataatt caccgcagag tgaacttaat ctattgatgg ccaatgaatc
     2101 tttatctgtg taaattcgtg taatctgtgg cgaatacgaa tttcgacact tatataagtc
     2161 aattcacttg tacggtccgg aaatattgcc tgaatagttt ttcagccttt tctatctgct
     2221 ccggagtatt ctctttcact cctttcagca tatattcttt gttcattgct tcatatttgt
     2281 gaacacccaa ggtgtggtaa ggcaatattt ctacacgctg aatcatctgg tacgttccaa
     2341 gatgctctcc cagctgccgg atatcgtctt cgaagtcact gtagccgggc actaatacgt
     2401 atcgcaacca gaatggttta tgattggttt caagccaggc tgcggttttc agggtctgct
     2461 cgttgcttct tccggttaaa gactgatgat gttccggatt aaattctttg atatctaata
     2521 aaaccagatc agtcaattcc aatagttcct ctacatcatt gttccatagc ccgccattgg
     2581 tatcaaggca aatatggatt cctgcttcct ttagtctttt aaaaagtggg ataagcgatt
     2641 ttgcttgaaa agtaggctct ccaccggaaa aggtgatacc tcccttttta ccgaagaaag
     2701 ctttctgact gacggccatg cgaactattt cctcagggtc ggttgctgtc cctcctttgg
     2761 cttcaattgt gtccggattg gcacaataga ggcaacggaa gttacaccct tgaaggaaaa
     2821 cgacgagccg gagacccggc ccgtcgaatg ttcccattga ctcgtatgag tgtacgttta
     2881 tcattcaatt acattctttc gtggaaactg cggctgatta cttccaactg atgttcgcgg
     2941 ctcaatttca cgaaattaac ggcatatcct gagacacgga tagtcagttg cggatacttt
     3001 tcgggatgtt ccatcgcatc ctgcaacatt tcccggttca gcacgtttac gttcaggtgg
     3061 tgtgcgcctt ttgtaaaata tccgtccatc atggtcacca gattgtcgat acggtcttct
     3121 tgtgttgctc ccaatgattt gggaacgatt gagaatgtat tgctgatacc atcctgtgag
     3181 tcgcgataac gcaacttggc tacagaactc agtgaggcga ttgcaccatt tttatcacgt
     3241 ccgtgcatgg ggttggcacc gggagcaaag gcaatgcctt tggcgcgtcc gtcaggagtg
     3301 gcaccggtct tcttgccata cattacgttc gaagtgatag tcagcagcga aagggtagga
     3361 cgggcattct tatatacggg gagtttcttt aattcttcgc taaagaaata taccaggtcc
     3421 acacccaggt ggtctacacg gtcatcgtca ttaccgaaac aagggaaagc accttcgata
     3481 tcgaatccct ccgtcagtcc gatgtcgttg cgacgggctt tcacatgtgc atatttgatg
     3541 gcggacagtg aatcgatggc gattgaaagt ccggctacac cataagcgag gttaatgcgg
     3601 ggatcagtat ccacgaaagc catctgcgcc ttttcgtagt aatacttatc gtgcatgtaa
     3661 tgtatgatat tcattgcttc gttgtataca cgtgcaattt gtatcaatac tttcttatag
     3721 ttggccatta cttcttcgaa cttcagtgtt tcaccggtca atacagggat gtcttttacc
     3781 ataactgtac ctgtattttc gcaacgtcct ccgttgatag ccagtagcag ggctttggca
     3841 aggttggcac gtgcgccgaa gaattgaatc tgctttccga tttcctgata tgaaacacaa
     3901 caagcgattc cgtagtcatc cgattgacgg acttcacgca ttaggtcgtc gttttcgtat
     3961 tgtacggaag aagtgtcgat agatacttgt gcacagaatg ctttgaaacc ttcgggtaac
     4021 tcagggctcc atagtacagt catgttaggt tccggtgacg ggcctaagtt ataaagtgtc
     4081 tgcaagaagc ggaaagaagt tttagtaact ttggtacgtc catcattgaa acgtccaccg
     4141 atagattcgg tcacccatgt gggatcaccc gcaaaaatat cgttgtacga ctgcatacgc
     4201 aagtggcgta ccatacggag tttgataacg aattggtcga tcagttcctg agcgaatgtt
     4261 tcggttatcg taccctggct taattcatat tcaagataga tgtcgaggaa tgaagataca
     4321 ttacccagtg acatggcagc accatcctgc tcttttacgg cagccagata agccatatac
     4381 acccattgta cagcttcctg tgcggtatat gccggtcggc tcagatccaa accataatat
     4441 tcgcccaata ctttaatctc tttcagggcc ttgatttgct cggctacttc ttcgcgaaga
     4501 cgaatgcggg cttcagtcat aggacctgtc agattatgca gatcttcttt tttagcttct
     4561 atcaggcggt caagaccata gagagccagg cggcgataat caccgatgat acgtccgcgt
     4621 gcatagttgt cgggaagtcc ggtaaggaat cccaatgaac ggaacgaacg aatttcttcg
     4681 gtatacacat cgaatacccc atcattatga gtcttgcgat agtgagtgaa gatatcctta
     4741 actctgtcat ctacttccac tccgttttca cggcaagctt tcataaccac attgattccg
     4801 ccaaacggtt tgatggcacg ttttaacacc tgatcggtct gtagtccgac gatcagttcg
     4861 ttttctttat cgatgtagcc ggcagggtga gaggttatag tagatacagt tgtgtagtcg
     4921 agtgcacgaa taccattatt gtttctctct tcttcgagag ctttgagaca gtgattccag
     4981 acagctttgg tgcgttcggt aggtccttgc aggaaagaag catccccttc ataaggagtg
     5041 atgttgttac taacgaaatc ccttacattg atttctttgc tccaaagtcc atctttaaag
     5101 gtcttattca gttccataaa attaatcagt tagtggttag ttatccttta gagcgcgcaa
     5161 atttacaacc ttttttcggt ttaggtatat aaaagggtcc tattttatgc tgaatagcat
     5221 aaaaatacct atttatgggg agaagccaga gggcggttat tacttccaaa tatacttttc
     5281 taatattttt ttgcggaata atttgcaagt taaaaaatga ttcgtacctt tgcagcgctt
     5341 taagagaaaa gcactactta accgtagttc tggagaggtg gcagagtggt cgattgcggc
     5401 ggtcttgaaa accgttgtac
//
