LOCUS       c00101_gnlPro..         6563 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DMKNCOBH_101.
ACCESSION   c00101_gnlPro..
VERSION     c00101_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 22:02:03
            Original ID  :: gnl|Prokka|DMKNCOBH_101
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6563
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1374
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg101_1"
                     /sec_met_domain="Gly_radical (E-value: 2.6e-47, bitscore:
                     149.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 9.2e-199, bitscore:
                     650.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRL
                     NDGRTKVTKTSFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYEN
                     DDLMREVRQSDDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVK
                     NIPVLTSDTLKFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPR
                     INLAYGVAGLSIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGV
                     DLVYFFSEELKKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMH
                     GRDKNGAIASLSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKG
                     AHHLNVNVLNRDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     protocluster    1..6563
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2116]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2116
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6563
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6563
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             1388..2116
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.5;
                     E-value: 1.4e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg101_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.7e-29, bitscore:
                     92.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(2109..2558)
                     /locus_tag="ctg101_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(2545..2712)
                     /locus_tag="ctg101_4"
                     /transl_table=11
                     /translation="MKEIVSRILDVIMYLIPFFGKRKRDRVVREVRYYTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             complement(2772..3275)
                     /locus_tag="ctg101_5"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLIFRPGKTLAELMKILSYQQVQTRAVAQAGAGDGGGEDPDKNPDSGGDGSGDEEAP
                     DPTV"
     CDS             3469..3699
                     /locus_tag="ctg101_6"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             3786..5900
                     /locus_tag="ctg101_7"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GEKAANEKKKELPAIAFSASYRGRRTKVNLVKYLGHIVIDIDHLSKEELARILPIIKRC
                     DYTRIAFISPKGMGVKIIVRACHPDETLPETLQEIEDFHHAAYTRLVSFYTELCRIEID
                     TSGQDVARTCLFSYDPEIYFNPNADAFLVDQPQASYKVSNRKNLSGSKQQTPPDGPPTN
                     EDTALNAHSANASLVLTLTYYHNKSEKYIAGNRNNYLHHLSCTFNRYGIPQEEASAFIK
                     SQFTDFPADETVSLINSAYAHTDEFNTCKLNGTQKRILRIEQYISEHYETRYNEVLHIM
                     EYRRRHPDTEKPEPFRILDEMMENSIWIEINELGYPCTVKTIENLIYSDFSQSYHPIRE
                     YFELLPEWDGTDYIRILADSVQTSHQEFWAECLERYLVAMCAAATQENIVNHTVLLLCS
                     EIQNIGKTTFINNLLPPELRAYLSTGLINSNNKDDLAKITQAMLINLDEFEGMSGRELN
                     AFKDLVTRKVISFRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHPYSIQF
                     ITINYAQLYAQIKYLLNKPGYQYWFTQADNERLEENNEAFIFHSPEEEMVLTHIRKPER
                     FEKVYYLTVSEIAELIRERTGYQYSIGSKIQLGKVMTKHHFESKKGNNGRRYAVFIIDI
                     EQVKSNRLYE"
     CDS             complement(5970..6272)
                     /locus_tag="ctg101_8"
                     /transl_table=11
                     /translation="MQINNHQIVDYDAVLDAKFGAEGTPERAEAEEKAYAFYTGQIIED
                     ARKKARITQAELARRIGSDRSYISRVESGQTEPKVSTFYRIMNALGCKIEFSMIL"
     CDS             complement(6483..6563)
                     /locus_tag="ctg101_9"
                     /transl_table=11
                     /translation="YGGYFEAFMETLTEKEQDRFNTDYSC"
ORIGIN
        1 gaatcattcg cgcaagagct gatcgaccag tttgtcatca aactgcgtat ggtccgccac
       61 ctgcgtatgc aatcatacaa cgacatcttt gcaggcgacc cgacctgggt aaccgaatct
      121 ctgggcggac gtctcaacga cggacgtacc aaagtgacaa agacctcctt ccgtttcctg
      181 cagacattgt acaacctcgg cccttcaccg gaaccgaacc tgaccgtact ctggagcccg
      241 gaacttccgg aaggattcaa agaattctgt gcaaaggttt ctatcgacac ttcttctatc
      301 cagtacgaaa atgacgacct gatgcgcgaa gtacgtcagt ccgacgacta cggaatcgcc
      361 tgctgcgtat cataccagga aataggtaag cagattcagt tcttcggtgc ccgttgcaac
      421 ctggccaaag ccctgttgct tgccatcaac ggcggacgtt gcgagaatac cggtacagtc
      481 atggtgaaga acatccccgt actgaccagt gacacgctga agttcgaaga agtaatggac
      541 aactacaaaa aagtgctgac cgaaatcgcc cgcgtatata acgaagcgat gaacatcatc
      601 cactatatgc acgacaagta ttactacgag aaggcccaaa tggctcttgt agatacgaac
      661 ccgcgcatca acctggctta cggtgtagcc ggactttcca tcgccctcga ttcactgtct
      721 gccatcaaat atgcgaaagt aactgcccgc cgcaatgata tcggtctgac agaaggcttc
      781 gacatcgaag gagaattccc ttgcttcggt aatgacaacg acaaggtaga ccaccttggc
      841 gtagacctgg tatacttctt cagcgaagaa ttgaagaaat tgcctgttta caagaatgcc
      901 cgtcctaccc tctccttgct gactatcact tccaatgtga tgtacggcaa aaagaccggt
      961 gctactcccg acggacgtgc caaaggtgtt gccttcgctc cgggtgccaa cccgatgcac
     1021 ggacgcgaca agaatggtgc gatcgcttct ctgagttctg tagcgaaact tcgttaccgt
     1081 gactcacagg acggtatcag caacaccttc tctatcgttc cgaaatcact gggtgctacg
     1141 gacgaagacc gtatcgagaa cctcgtaacg atgatggacg gttacttcac caaaggtgcc
     1201 caccacctga acgtaaacgt actgaaccgt gatatgctgt acgatgccat ggagcatccg
     1261 gaaaactatc cgcagctgac tatccgcgtt tccggctatg ccgtaaactt cgtgaagctg
     1321 agccgcgaac accaactgga agttatcagc cgtagcttcc acgaacgtat gtaatctatt
     1381 actctatatg atgataaacg tacattcata cgaaagtatg ggaacattcg acgggccggg
     1441 cttacggctc gtcgtttttc ttcaaggatg caacttccgc tgcctctatt gcgccaatcc
     1501 ggatacgata gcaggaaagg gaggcacgcc taccccaccg gaagaaatcg tccgcatggc
     1561 aatgagccag cgccccttct tcgggaaacg aggaggaatc actttctccg gaggcgaacc
     1621 gacgtttcag gcgaaagcac tcgtcccgct ggtccgcgaa ctgaaagaaa aaggcatcca
     1681 tgtctgtctg gacagcaacg gaggactttg gaacgaagac gtagaagaat tattcagact
     1741 gaccgatctt gtgttattgg atataaagga attcaacccc aaccgccatc agacgctcac
     1801 cggaagaagc aacgagcaaa ccatccgtac ggccgcctgg ctggaagaac aggagaaacc
     1861 tttctggcta cgctacgtgc tagtgcccgg atacagcgat tttgaagaag acatacgcgc
     1921 actgggagaa gcactcggaa aatacaagat gatccaacgg gtggaaatac tgccttacca
     1981 tacactgggc gtacataagt acgaagcgat gggacaggaa tacaagatga aaggggtaaa
     2041 agaaaatacc cccgaacagc ttgaaaaagc tgcggaggta ttcaaagagt atttcactac
     2101 ggtggtagtt aattaatgga gaattctcca atgacctcaa agcaagggca ttctttaatc
     2161 cgttcccaag gatctacgat cccattatag ttggtgtcgg gacttaaatc ccgatgccct
     2221 actactttag cttccggata acgctgcaac aattcgtgta ccaagcgatg tagcgcctcc
     2281 ttctgacggg gtgtacgcgt atccgccgct tttccattct catccagacc gccctcatag
     2341 catacaccta agctgatcgc attatagcca cgtgcatgag ctccgaccgt atcttcatcc
     2401 cgcatcggct caatctcgcc cgacttgcgt atataataat gataccccca gcacgaaaag
     2461 ccacgataac ggtgagccgt atccacatcc tttgccgtaa agtcgcaatc gacacgggtg
     2521 gcggaacaat ggatcacaat cagactaatc tttctcatgt tccttttccc tttccgtcgt
     2581 tttcactttg cacacctcct tacaagtagt ataatagcgc acttctctca ccaccctgtc
     2641 ccgctttctc tttccgaaga aaggaatcag atacatgatc acatctaaaa tccgacttac
     2701 tatttctttc atcttatcaa aatattaatg gaagttattc taattcgtta ttaccttgac
     2761 ccgtctgcca attataccgt agggtccgga gcctcttcat caccggagcc atcaccaccg
     2821 gaatccggat tcttgtcagg gtcttctccc ccgccatcgc ctgcacccgc ctgcgccacc
     2881 gcacgagtct gcacttgctg ataactcaga atcttcatca actccgccag cgtcttaccg
     2941 gggcggaaaa taagatgcgc ccccttaatc attgaaccgg aaaattcctt gaccgatgcc
     3001 gcaccttcac tgttgagtcc tatctgcaaa ctgcccaaat cgcccagtct cgcaattctt
     3061 cctgccttca acgcacgctt cacttcgtaa agaatacccg aaatagccgc acgtacatcc
     3121 gtttccgtac aagtagaacg actggtgatc gcttcgcaca actcttcaaa gtccaactct
     3181 cccgacgcct gcgcctgtgc ataatacttt gccggttcgc tcttttttgc aggattcttg
     3241 cgcggcacca ctgaataaga tactgtcatt gccataataa aataatttta aagtttataa
     3301 tacataggat gcatcccctt cttgttaaca gcacaaagat aaggatagcc ggacaagcca
     3361 tattccccaa cgaagtttct tgattcccaa cgaataaaaa cgaagtcaaa cctttgaata
     3421 acaaggaatt ttatgtaact ttatagcata aaacaagaag aaaacatcat gaattacaaa
     3481 gaagattctt tccttatccg cacctatacc aaagcggaac tggctcacct ttacaatccc
     3541 catgtctgcc tcaaagttgc tttacaaata cttcgtcgtt ggattattta caacctccct
     3601 ctcctgcacg aactcgaaca ggaaggctac cgcgcacgca accgtctcct ttcgccgaaa
     3661 caagtggcaa ctatcataca ctttttgggc gaaccgtagt aacttattgc cattttatac
     3721 aaagttcctt tacacatttc cgcaaagaag ttcctttgca tcgacaaaca ataatcaaaa
     3781 taaaaatgaa acatttcacc atatttcaag gtttctacta cgcaatagca gagatgaccg
     3841 aagaagaaat agtgtccact atcggcagtt ttacctaccg ggaaaaagtc gaagaaatac
     3901 gccgcatctt tgcagagcaa ggagaaaagg ctgccaatga gaagaaaaaa gaattgccgg
     3961 caatcgcctt ctccgccagc taccgaggaa ggcgtaccaa agtgaacctc gtcaaatatc
     4021 tgggacacat cgtgatagac atcgaccacc tcagcaaaga agaattggca cgcatcctcc
     4081 ctatcatcaa gagatgcgat tacacccgca tcgcctttat cagtcccaaa gggatgggcg
     4141 tgaaaatcat agtacgcgca tgccatccgg atgagacatt gcccgaaacc cttcaggaaa
     4201 tagaagactt ccatcatgca gcttacacca gactcgtttc tttttacacc gaactctgtc
     4261 ggatagaaat cgacacctcc ggtcaggatg tggcacgcac ttgtctgttc tcttacgatc
     4321 cggagattta tttcaatccg aacgctgacg cttttcttgt agatcaacct caggcatcct
     4381 acaaggtttc aaatcgtaaa aatctctccg gcagtaaaca acagactcct ccggacggcc
     4441 ccccgacgaa tgaagacaca gcgctcaacg cccattccgc caacgcttct ctggtgctga
     4501 ctttaaccta ctatcacaac aagtccgaaa aatacatagc gggcaaccgg aacaattatc
     4561 tgcaccatct ctcatgtacc ttcaatcgct acggcattcc gcaggaggaa gcatccgctt
     4621 ttatcaaaag ccagtttaca gactttccgg cagacgagac agtctctttg atcaatagcg
     4681 cctacgctca taccgacgaa tttaatacct gtaagctgaa tggcacgcag aaacgaatat
     4741 tacggataga gcaatatatc agcgagcact acgaaacacg ctacaacgaa gtgctgcata
     4801 tcatggaata tcgcagaagg catcccgaca ccgagaaacc ggagcctttc cgcatcttgg
     4861 atgagatgat ggaaaacagt atctggatag aaatcaacga actgggatat ccctgcactg
     4921 tcaaaacaat cgaaaatctg atttactccg atttcagcca gtcgtatcat cccattcgcg
     4981 agtattttga attactgccc gaatgggacg gaaccgacta tatccgtatc ctggcggata
     5041 gtgtccagac gagtcatcag gagttttggg cggaatgcct ggaacgttat ctggtggcga
     5101 tgtgcgcagc ggctactcag gagaatattg tcaatcacac ggtgcttctg ctatgcagcg
     5161 agatacagaa cataggaaag actaccttta tcaataatct cctgcctccc gaactgcgtg
     5221 cttatctctc taccggacta atcaattcga acaataaaga tgatttagcc aaaataacgc
     5281 aggctatgct gatcaatctc gacgagtttg aagggatgag cggccgggaa ctgaatgcat
     5341 tcaaagattt ggtaacgcgc aaagtgatca gtttccgtct gccttatgca cgccgttcgc
     5401 aaaacttccc tcataccgcc tcctttgcag gaacctgcaa ttatcaggaa gtgcttcacg
     5461 acacgacagg caaccggcgt ttcctttgtt ttcatcctta ttccatccaa tttattacca
     5521 tcaattatgc gcagctctat gctcagatca aatatctgct gaataagccg gggtaccaat
     5581 actggttcac gcaagcggac aatgaacggc ttgaagagaa caacgaggct ttcatctttc
     5641 attcaccgga agaggaaatg gttcttacgc atatccgcaa gccggaacgt ttcgagaagg
     5701 tttattatct gactgtcagt gagattgcgg aactaatacg tgagcgtacc ggttatcagt
     5761 attccatcgg atcaaaaata cagttgggga aggtgatgac taagcaccat tttgaatcca
     5821 aaaaaggaaa taacgggcgc cgatatgccg tatttattat agatatcgag caggtaaaat
     5881 caaacaggct gtatgaatag aagcaggaaa gccttgtgaa tatagcaaaa tgcagagtca
     5941 tcttgacaga tactctgcat ttattgtttt tacaaaatca ttgaaaattc gattttacag
     6001 cctaacgcat tcatgatacg atagaaagta gatactttag gctctgtctg accactttct
     6061 actcttgaaa tataagaacg gtcagaaccg atacgccggg ctaattcagc ctgagtgatt
     6121 ctagcctttt ttcttgcgtc ctcgataatc tgtcctgtat aaaaggcgta ggccttttct
     6181 tctgcctctg cacgctcggg agtaccttca gcaccaaatt tggcatcaag gacagcatca
     6241 taatctacta tttgatgatt gtttatctgc ataatattcc tcctttattt taactgcttt
     6301 atcaatttca tgaccaggtg ttttttgtga cttcttttga aagccattga accacacaac
     6361 aatattacca tcatcaaata tgaaaaatac ccggtatata ttgttattat attctgtgcg
     6421 aagttcaaaa actccatccc gaataaactt cacaaattta gcggagagtc tttcttgtgt
     6481 tttcaacaag agtaatccgt attgaatcta tcttgttcct tttcggttaa agtctccatg
     6541 aaagcctcaa agtaacctcc gta
//
