LOCUS       c00016_gnlPro..         3139 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MLFNLBCE_16.
ACCESSION   c00016_gnlPro..
VERSION     c00016_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 18:02:29
            Original ID  :: gnl|Prokka|MLFNLBCE_16
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3139
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3139
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:3025]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..3025
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3139
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3139
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..728)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 77.8;
                     E-value: 3.2e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg16_1"
                     /sec_met_domain="Radical_SAM (E-value: 3.7e-29, bitscore:
                     89.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIDTKGE
                     STETTIDEIVRMAVSQKAFFGKKGGVTFSGGEPTLQAKALIPLFQRLKEQSIHICIDTN
                     GSIWNEEVKELLQWTDLVLLDIKEFNNVRHRQLTERSNEQTIRTAGWLEKNGKPFWLRY
                     VLVPGYSSFEEDIRALGEHFKNYQMIQRVEILPYHTLGVHKYEAMGKEYKLNGIKENTL
                     KQLETAKTLFGEYFNTVYLN"
     CDS             complement(797..3025)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg16_2"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-48, bitscore:
                     151.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEMNKVFKDGLWSKEINVSDFVHTNITPYEGDASFLAGPTERTKK
                     VWNECLKALEEERANNGVRSLDNVTVSTITSHKAGYIDRENELIVGLQTDELLRRAIKP
                     YGGIKVVEKACHENGVEVDEKVKDIFTHYRKTHNDGVFDAYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLHNITSPMTDARIRLREEVAEQIKALKEIK
                     ILGEYYGLDLGRPATNAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYIQYDLDH
                     GKIDESFAQELVDQFIIKLRMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKEFCAQVSIDTSSIQYENDTLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKDIPVLTGDLL
                     NFEEVWSNYKKVLMQIARVYNDAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYGKVRAKRNDIGLTEGFDIENAYPCFGNDDDRVDHLGVDLVYFFSEEL
                     KKHPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDAQDGISNTFSIVPKSLGVDQETRIENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFIKLSREHQLEVISRSFHERM"
ORIGIN
        1 taatttaaat atacggtatt gaaatattca ccaaataacg ttttggctgt ttccaattgt
       61 tttagagtat tttcttttat tccgtttagt ttgtattctt ttcccatggc ttcatatttg
      121 tgtacgccta atgtatgata aggtagtatt tctactcttt gaatcatttg ataattcttg
      181 aagtgctctc ctaatgcccg tatatcttcc tcaaaggaac tatagcctgg aactaataca
      241 tagcgcaacc aaaaaggctt tccgtttttt tcaagccatc cggcggtacg tatggtttgt
      301 tcgttgctgc gttctgtcag ttgccgatgg cgcacattat taaattcctt gatgtctaat
      361 aatacgaggt ctgtccattg tagcagttcc tttacttctt cattccatat actgccgttg
      421 gtatctatac agatgtggat gctttgttct tttaatcgct ggaataacgg aatcagtgct
      481 tttgcttgca atgtaggctc gccgccgctg aaagtcacac ctcctttttt accgaaaaaa
      541 gctttttggc tgacggccat acgcacaatt tcgtctattg ttgtttccgt actttcacct
      601 ttggtgtcaa ttgtgtcggg gttggcacaa tagaggcatc ggaaattgca tccttgtaaa
      661 aatacgacta agcgtaaacc gggaccgtca aacgtcccca ttgactcgta agaatggact
      721 ctgatcataa tggtttgtct ttaaactggt aagggcaggt ttaaaacccg cccctacgat
      781 gaatgtatat gtgatgttac attctttcgt ggaaagaacg gctgattact tccagctgat
      841 gttcacggct caatttgatg aagtttacgg cataacctga tacgcggatg gttagttgcg
      901 ggtatttttc aggatgttcc attgcatctt ccaacatttc acggttcagc acatttacat
      961 tcagatgatg tgctcctttg gtgaaataac cgtccatcat ggttaccagg ttctctatac
     1021 gggtttcctg gtctacccct agtgattttg gtacgataga gaacgtgtta ctgatgccgt
     1081 cctgggcatc acgatagcgt agtttggcta cagaactcaa tgaggcgatg gcaccgttct
     1141 tgtcacgtcc gtgcatcggg ttggcacccg gagcgaaagc cactcctttg gcacgaccat
     1201 caggagtggc acctgttttc ttgccgtaca ttacattgga ggtgatagtc agcagtgaca
     1261 gggtagggcg ggcatttttg tataccggat gtttctttaa ttcttcactg aagaaataaa
     1321 ccaaatctac ccctaagtgg tctacgcggt catcatcatt accgaagcaa ggatatgcat
     1381 tttcaatatc aaagccttcg gtcaaaccta tatcattacg tttggcacgt actttgccgt
     1441 atttgatggc agacaatgag tcaatggcaa tggacagacc ggctacgcca tatgccagat
     1501 tgatacgtgg atcggtatca atgaatgcca tttgagcctt ttcataataa tatttatcgt
     1561 gcatatagtg aatgatattc atcgcatcat tatacacacg tgcaatttgc attaatactt
     1621 tcttataatt agaccatact tcttcaaagt tcagcaagtc ccctgtcaat acaggaatat
     1681 ctttcaccat tacagttcct gtattctcac aacgtccgcc attgatagcc agcaataggg
     1741 ctttagccag attgcaacgt gctccgaaga actggatttg tttgccgata gcctgataag
     1801 aaacgcagca ggcgatacca taatcatcac agttacgcac ttcgcgcatc agcgtgtcgt
     1861 tttcgtattg tatagaagaa gtgtcgatag aaacttgtgc acagaattct ttgaaacctt
     1921 ccggcaattc cggactccac agtacagtca tattaggttc gggtgacgga cccagattgt
     1981 atagtgtctg caagaaacgg aaggaagttt ttgtaacctt ggtacgtccg tcgttgaagc
     2041 gtccgccgat agactcggtt acccaggtgg gatcacctgc aaagatatcg ttgtatgatt
     2101 gcatacgcag atgacggacc atacgcaact tgataatgaa ctggtctacc agttcctgcg
     2161 caaatgactc gtcaatttta ccatgatcca aatcatattg aatatagata tccaggaatg
     2221 aagaaacgtt gcccaatgac atagctgctc catcttgttc tttcacggca gcaagatatg
     2281 ccatataaac ccactgaaca gcttcttgtg cattagtagc gggacgaccc aagtcgagtc
     2341 cgtaatattc acccaatatc tttatttctt tcagtgcttt gatttgttcg gctacttctt
     2401 cgcgcaggcg gatacgggca tcggtcatcg gactggtgat attgtgcaaa tcttcttgtt
     2461 ttgcttcaat cagtcggtcg ataccataca gtgccaaacg gcggtagtca ccgatgatac
     2521 gcccgcgtgc ataattatcg ggcagaccgg tcaggaaacc gagtgaacgg aaagagcgga
     2581 tttcttcggt gtaagcatca aatacgccat cattatgggt tttgcgataa tgagtaaaga
     2641 tatcctttac tttttcatct acttccacac cgttttcatg gcaggctttt tctacgactt
     2701 tgataccacc ataaggtttg atggcacggc gcaaaagttc atccgtttga agacctacaa
     2761 tcagttcgtt ttcacggtca atgtatcctg ctttatgtga ggtgatggta gatactgtta
     2821 cattatcaag ggaacgaact ccgttattgg cacgttcttc ttctagtgcc ttgaggcatt
     2881 cgttccacac ttttttagtt cgctctgtgg ggcctgccag aaatgaggca tcgccctcat
     2941 aaggggtaat gttggtatgt acgaagtctg aaacattaat ttccttactc cacaatccat
     3001 ccttaaaaac tttgttcatt tccatactgt tattccatta ttaagagatt tagggctgca
     3061 aaggtactcc ttttttgtga gctatgcatc actattagta ggtataatgc gaatacttgt
     3121 gcatttttaa gtataaagt
//
