LOCUS       c00229_gnlPro..         8159 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ONFMHJLC_229.
ACCESSION   c00229_gnlPro..
VERSION     c00229_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 22:31:27
            Original ID  :: gnl|Prokka|ONFMHJLC_229
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8159
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..8159
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3200:6226]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3201..6226
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..8159
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..8159
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(234..914)
                     /locus_tag="ctg229_1"
                     /transl_table=11
                     /translation="MKEYRLRVKEKIMKADLATIRREYTKGGLKRSDLPDNPLVLFTKW
                     LQEAIDAEVNEPTAMLVGTVSPEGKPAIRTVLLKDLHDGKFIFYTNYGSRKGKHLAHNP
                     SISISFVWHELERQIHIEGIAAKVSPQESDEYFRKRPYKSRIGARISPQSEPIESRIQL
                     IRDFVKETTRWIGKEIDRPEYWGGFAVMPVRVEFWQGRVNRLHDRFLYMLQSDGVWKIE
                     RLAP"
     CDS             1340..2434
                     /locus_tag="ctg229_2"
                     /transl_table=11
                     /translation="MKILLIGEYSNIHWTLAKALRNMGHRVDVLSHSNLWLNNSDYLYP
                     NRSSIRWGVLKNTFNLLKTLPQLRGYDVVQLVNPYFLELKPELLQYVFQYLKKNNKKIF
                     LGGFEYDFYWIYMCLNKNALRYNDFYANGTFRDTIDNKMAIINWMHGFRGKLNRIIANN
                     CNGIITSLYESYLAYQPYFSGKTKYIPFPIESLPHPQIPFKKIEKVKFYISLKHHREEW
                     KGKDELYSALYKLYYRHPSACEIKQTVFTPYDECEHFMDGCDVMLDQLYSYSPGLNALY
                     AMSKGIVVVGGAEEEHYNLLGEDRLRPIINVRPEGNDIYNKLESLLANTNKISQLSADS
                     IEYIKKHHCPIKVAKECLDFWEKN"
     CDS             2407..2562
                     /locus_tag="ctg229_3"
                     /transl_table=11
                     /translation="MFGFLGEKLIPPINTSLSAPYYFHQKIYLKKKSKRFAYFNLSPYL
                     CTAFER"
     CDS             3201..5429
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg229_4"
                     /sec_met_domain="Gly_radical (E-value: 5.3e-48, bitscore:
                     151.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1169.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEMNKVFKDGLWSKEINVSDFVHTNITPYEGDASFLAGPTERTKK
                     VWNECLKALEEERANNGVRSLDNVTVSTITSHKAGYIDRENELIVGLQTDELLRRAIKP
                     YGGIKVVEKACHENGAEVDEKVKDIFTHYRKTHNDGVFDAYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLHNITSPMTDARIRLREEVAEQIKALKEIK
                     ILGEYYGLDLGRPATNAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYIQYDLDH
                     GKIDESFAQELVDQFIIKLRMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKEFCAQVSIDTSSIQYENDTLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKDIPVLTGDLL
                     NFEEVWSNYKKVLMQIARVYNDAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYGKVRAKRNDIGLTEGFDIENAYPCFGNDDDRVDHLGVDLVYFFSEEL
                     KKHPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDAQDGISNTFSIVPKSLGVDQETRIENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFIKLSREHQLEVISRSFHERM"
     CDS             5498..6226
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 77.8;
                     E-value: 3.2e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg229_5"
                     /sec_met_domain="Radical_SAM (E-value: 1.5e-28, bitscore:
                     89.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIDTKGE
                     STETTIDEIVRMAVSQKAFFGKKGGVTFSGGEPTLQAKALIPLFQRLKEQSIHICIDTN
                     GSIWNEEVKELLQWTDLVLLDIKEFNNVRHRQLTERSNEQTIRTAGWLEKNGKPFWLRY
                     VLVPGYSSFEEDIRALGEHFKNYQMIQRVEILPYHTLGVHKYEAMGKEYKLNGIKENTL
                     KQLETAKTLFGEYFNTVYLN"
     CDS             6328..7077
                     /locus_tag="ctg229_6"
                     /transl_table=11
                     /translation="MKRLLLLCLTYIMCVGNHQMAAQDSSESPGIFNSLAVGVSASTTG
                     IGIDVASPIGSYLAVRAGVSFMPNFTYSTDVDVDIEGSYEGNIPTSIDVEGGMGRTAGE
                     ILLNVYPFKSSSFFVCGGAYFGGGKLIKIKGHSDELAELIKEGQEVGIEIGNYRIPVDQ
                     NGNISGGLKVSSFRPYLGLGFGRAIPKKRIGFMFEMGVQFHNTPKVYTNNGDLSDLTAE
                     ADNDFTDIIDKLTVYPVLKFRLCGKIF"
     CDS             complement(7156..7743)
                     /locus_tag="ctg229_7"
                     /transl_table=11
                     /translation="MSSREDILQSIRRATHVKYEKPDLKPLEDMALTYPSKVEQFYLIM
                     KQVGGEAILLEKEEDVNDFIKKAYPGAKRIASNLKTITCATFNPDDVEDPAELNGTDLA
                     VIDGKIGVAENGAVWIEQDVKQRAIYFIAEKLVILLNKNKIVNNMHEAYKLIDTGEYGF
                     GTFISGPSKTADIEQALVMGAHGARDVMVVLI"
     CDS             complement(7747..8157)
                     /locus_tag="ctg229_8"
                     /transl_table=11
                     /translation="GGYSYTYFIPGPIGINLGMLKAPLHYYDNVSACSLCYSCSFVCPA
                     KVDLAEQIYLWRQDLDKLGKADRMKRVMSGGMEFMMNRPLIFNMALKWAPLVNGMPRFL
                     IYNGLNDWGKGREMPKFAKESFNEMWKKGKVK"
ORIGIN
        1 tagtcagcgg tctgcaaaac cgtgtacggc ggttcgaatc cgccaggaac ctcctcatca
       61 cttcaaaaat aaatatgcaa aagggaataa tccatagatt attccctttt gcatataata
      121 tataaaaagc aacgccttca attactcaaa agtattccta tttacaccta cattcaaaag
      181 gtttctcggc tcagtttgaa aaagaacctg gaattatttc tctattccga agattaagga
      241 gcgagtcttt ctattttcca aacaccatca gattgaagca tatagagaaa acgatcatgc
      301 aaacgattta ccctaccctg ccagaattca acacgaacag gcataacagc aaagcctccc
      361 cagtattcag ggcgatctat ctctttccct atccagcgcg tggtttcttt cacaaaatca
      421 cgaataagtt gtatcctgct ttctatcggt tcgctttgag gagaaatacg ggcgccaata
      481 cgactcttgt aaggcctttt tctaaaatat tcatctgatt cctgtggaga tactttagcc
      541 gcaatacctt ctatatgaat ttgcctttcc agttcatgcc atacaaacga tatagagata
      601 gatgggttat gagccagatg tttacccttg cgacttccat agttagtata aaaaataaat
      661 ttgccatcat gcaaatcttt caacaacaca gtccgtattg ccggtttccc ttccggagaa
      721 acagtaccta ccaacatagc cgtaggctca tttacctccg catctatcgc ttcttgcagc
      781 cactttgtaa aaaggacaag tggattatca ggaagatcac tccttttgag accacccttt
      841 gtatattccc tcctaattgt cgccaaatca gccttcatta ttttttcttt aactcttaac
      901 ctatattctt tcattaacac ttaaatattc tttactcaat aaattcccca aaagaccata
      961 tcaaaatgga caccatacct cacataagta tgaaaacaat cttctaaaag aaaaggttta
     1021 gcaatttctc accgctatgc ataattgaag aaggacttat tttcattttt ttgaaataca
     1081 aaatcttttt gcaaaccata cattttcaat cattccacta cacacatttt tctgtaatac
     1141 aagaagatat atttttgata gatttaattt tcatagacaa tactagctac aacagaagac
     1201 aaattatcct tttttattgc aaaatcaccc tacaataaga actaaagcct acacttgttt
     1261 gttaaataga aaaataagaa tagcaaaacg acttgctacc aatgcacaaa ctatttaatt
     1321 aaataatgat tgactgttta tgaaaatctt attaatagga gagtatagca atatacattg
     1381 gactttagca aaagccttaa gaaatatggg acatagagtc gatgtattat cccacagcaa
     1441 tttatggcta aacaactctg actatctcta tcccaaccgg tcatcaatcc gttggggagt
     1501 ccttaaaaac acgttcaatt tactgaaaac ccttccccaa ctcagaggat atgatgtggt
     1561 acaacttgtc aacccttact ttttagaatt aaaacccgaa ctgcttcagt acgtattcca
     1621 atatcttaaa aagaacaaca agaagatatt tttaggaggg tttgaatatg atttttactg
     1681 gatttacatg tgcttaaata aaaacgctct ccgatataat gatttttatg ccaatggcac
     1741 ttttcgtgac accattgaca acaaaatggc aattataaat tggatgcatg gattcagagg
     1801 aaaattaaac cgtatcatcg cgaacaattg taatggaatc atcaccagtc tttacgaatc
     1861 ctatttagcc taccaaccct atttctctgg aaaaaccaaa tacattcctt tcccgataga
     1921 atcattgcct cacccacaaa tcccttttaa aaaaatagaa aaagtaaaat tctatatcag
     1981 tctaaaacat catcgtgaag aatggaaggg aaaagacgaa ctttatagcg ccctctataa
     2041 attatattac agacatccat ctgcatgcga aatcaaacaa accgttttca ctccgtatga
     2101 tgaatgtgaa cattttatgg acgggtgcga tgtaatgtta gatcagcttt attcttattc
     2161 tccgggatta aatgcattat acgccatgag caaaggaatt gtagtggtgg gaggtgcaga
     2221 agaggaacat tacaacttat taggcgaaga cagacttcgc cctatcatca acgtacgccc
     2281 cgaaggaaat gacatttata acaaactaga gagtttatta gccaatacaa ataaaatttc
     2341 tcaattatct gccgacagca ttgaatatat taaaaaacac cattgtccaa taaaggtggc
     2401 taaagaatgt ttggattttt gggagaaaaa ttaattccac ctattaacac ttcactatca
     2461 gcaccttatt atttccatca aaaaatatat ctaaaaaaaa agagcaaaag gtttgcatat
     2521 ttcaatttat ccccttatct ttgcaccgcg tttgagagat aacgcttctg ataagaagga
     2581 agtttgggtg agtggctgaa accaccagtt tgctaaactg acgtacgggt aaccgtaccg
     2641 ggggttcgaa tcccccagct tccgcgaata agtcgtaaat taattttacg acttattttt
     2701 tatattcatt tttcaacagt ttgctgtcaa ggctttcggg aggaagagta aaggtgcttt
     2761 ttagactttc aattttacta cataaaacat atgatacttc ttcatagaaa ttaaattccg
     2821 atttataata gtaatagcat aaaaaaaatc attttttatt ttgcagatta aaaacaaact
     2881 tctaactttg caccgtctta gaaaaagaat taagactaac tgatggcggt atcgtctagt
     2941 ggcctaggac gcagccctct cacggctgaa actcgggttc gattcccggt tccgctactt
     3001 ttagaagcta attggtttat tgtcaccaat tagctttatt ttttatggaa ttgttataaa
     3061 gagtccttat tgttttttca tttattgacc actttatact taaaaatgca caagtattcg
     3121 cattatacct actaatagtg atgcatagct cacaaaaaag gagtaccttt gcagccctaa
     3181 atcatcttaa taatgacagt atggaaatga acaaagtttt taaggatgga ttgtggagta
     3241 aggaaattaa tgtttcagac ttcgtacata ccaacattac cccttatgag ggcgatgcct
     3301 catttctggc aggccccaca gagcgaacta aaaaagtgtg gaacgaatgc ctcaaggcac
     3361 tagaagaaga acgtgccaat aacggagttc gttcccttga taatgtaaca gtatctacca
     3421 tcacctcaca taaagcagga tacattgacc gtgaaaacga actgattgta ggtcttcaaa
     3481 cggatgaact tttgcgccgt gccatcaaac cttatggtgg tatcaaagtc gtagaaaaag
     3541 cctgccatga aaacggtgcg gaagtagatg aaaaagtaaa ggatatcttt actcattatc
     3601 gcaaaaccca taatgatggc gtatttgatg cttacaccga agaaatccgc tctttccgtt
     3661 cactcggttt cctgaccggt ctgcccgata attatgcacg cgggcgtatc atcggtgact
     3721 accgccgttt ggcactgtat ggtatcgacc gactgattga agcaaaacaa gaagatttgc
     3781 acaatatcac cagtccgatg accgatgccc gtatccgcct gcgcgaagaa gtagccgaac
     3841 aaatcaaagc actgaaagaa ataaagatat tgggtgaata ttacggactc gacttgggtc
     3901 gtcccgctac taatgcacaa gaagctgttc agtgggttta tatggcatat cttgctgccg
     3961 tgaaagaaca agatggagca gctatgtcat tgggcaacgt ttcttcattc ctggatatct
     4021 atattcaata tgatttggat catggtaaaa ttgacgagtc atttgcgcag gaactggtag
     4081 accagttcat tatcaagttg cgtatggtcc gtcatctgcg tatgcaatca tacaacgata
     4141 tctttgcagg tgatcccacc tgggtaaccg agtctatcgg cggacgcttc aacgacggac
     4201 gtaccaaggt tacaaaaact tccttccgtt tcttgcagac actatacaat ctgggtccgt
     4261 cacccgaacc taatatgact gtactgtgga gtccggaatt gccggaaggt ttcaaagaat
     4321 tctgtgcaca agtttctatc gacacttctt ctatacaata cgaaaacgac acgctgatgc
     4381 gcgaagtgcg taactgtgat gattatggta tcgcctgctg cgtttcttat caggctatcg
     4441 gcaaacaaat ccagttcttc ggagcacgtt gcaatctggc taaagcccta ttgctggcta
     4501 tcaatggcgg acgttgtgag aatacaggaa ctgtaatggt gaaagatatt cctgtattga
     4561 caggggactt gctgaacttt gaagaagtat ggtctaatta taagaaagta ttaatgcaaa
     4621 ttgcacgtgt gtataatgat gcgatgaata tcattcacta tatgcacgat aaatattatt
     4681 atgaaaaggc tcaaatggca ttcattgata ccgatccacg tatcaatctg gcatatggcg
     4741 tagccggtct gtccattgcc attgactcat tgtctgccat caaatacggc aaagtacgtg
     4801 ccaaacgtaa tgatataggt ttgaccgaag gctttgatat tgaaaatgca tatccttgct
     4861 tcggtaatga tgatgaccgc gtagaccact taggggtaga tttggtttat ttcttcagtg
     4921 aagaattaaa gaaacatccg gtatacaaaa atgcccgccc taccctgtca ctgctgacta
     4981 tcacctccaa tgtaatgtac ggcaagaaaa caggtgccac tcctgatggt cgtgccaaag
     5041 gagtggcttt cgctccgggt gccaacccga tgcacggacg tgacaagaac ggtgccatcg
     5101 cctcattgag ttctgtagcc aaactacgct atcgtgatgc ccaggacggc atcagtaaca
     5161 cgttctctat cgtaccaaaa tcactagggg tagaccagga aacccgtata gagaacctgg
     5221 taaccatgat ggacggttat ttcaccaaag gagcacatca tctgaatgta aatgtgctga
     5281 accgtgaaat gttggaagat gcaatggaac atcctgaaaa atacccgcaa ctaaccatcc
     5341 gcgtatcagg ttatgccgta aacttcatca aattgagccg tgaacatcag ctggaagtaa
     5401 tcagccgttc tttccacgaa agaatgtaac atcacatata cattcatcgt aggggcgggt
     5461 tttaaacctg cccttaccag tttaaagaca aaccattatg atcagagtcc attcttacga
     5521 gtcaatgggg acgtttgacg gtcccggttt acgcttagtc gtatttttac aaggatgcaa
     5581 tttccgatgc ctctattgtg ccaaccccga cacaattgac accaaaggtg aaagtacgga
     5641 aacaacaata gacgaaattg tgcgtatggc cgtcagccaa aaagcttttt tcggtaaaaa
     5701 aggaggtgtg actttcagcg gcggcgagcc tacattgcaa gcaaaagcac tgattccgtt
     5761 attccagcga ttaaaagaac aaagcatcca catctgtata gataccaacg gcagtatatg
     5821 gaatgaagaa gtaaaggaac tgctacaatg gacagacctc gtattattag acatcaagga
     5881 atttaataat gtgcgccatc ggcaactgac agaacgcagc aacgaacaaa ccatacgtac
     5941 cgccggatgg cttgaaaaaa acggaaagcc tttttggttg cgctatgtat tagttccagg
     6001 ctatagttcc tttgaggaag atatacgggc attaggagag cacttcaaga attatcaaat
     6061 gattcaaaga gtagaaatac taccttatca tacattaggc gtacacaaat atgaagccat
     6121 gggaaaagaa tacaaactaa acggaataaa agaaaatact ctaaaacaat tggaaacagc
     6181 caaaacgtta tttggtgaat atttcaatac cgtatattta aattaaaaat atactgaaga
     6241 caagatgttg ctataaatca atggaaatat ttcatcattt caataaaatt atctaatttc
     6301 gcatctaaaa cataaattat tataaatatg aaaagactac tattactctg cctaacttac
     6361 attatgtgtg taggcaacca tcagatggct gctcaagact cttctgaaag tcctggtatt
     6421 tttaattcat tagctgtagg cgtcagtgca agtaccacag gtataggtat tgatgtagct
     6481 agtcctatag gaagttactt ggctgtacgt gcaggagttt cttttatgcc gaactttact
     6541 tattctacag atgtggatgt agatattgaa ggttcttatg aaggaaatat acctaccagc
     6601 atagatgtag aaggaggaat gggacgtact gccggtgaaa tattgctgaa tgtatatcct
     6661 ttcaaatcat ctagtttttt tgtatgcggt ggtgcttatt tcggaggagg aaaactcatc
     6721 aagattaaag ggcacagcga tgagttggca gaactgatta aagaaggaca agaagtaggt
     6781 attgaaatcg gaaactatcg tatacctgta gatcagaacg gaaatatatc tggtggtttg
     6841 aaggtttctt cattccgtcc ttatcttgga ctaggtttcg gaagagcgat tcctaagaaa
     6901 cgaattggct tcatgttcga aatgggtgtg caattccata acactcccaa agtatatacc
     6961 aataacggag atttgtctga tttaacagca gaagctgata atgatttcac tgatatcata
     7021 gacaaactta ctgtctatcc ggtacttaag ttccgtttgt gtggcaaaat attctaataa
     7081 tattctataa aataaaagga gatgagccct ataattttcg cctcatctcc tttttatata
     7141 taagaaacaa tacacttata tcagcaccac catcacatca cgtgccccat gagcacccat
     7201 taccaaagcc tgctcaatat cagccgtctt tgacggaccg gaaataaagg ttccgaagcc
     7261 atattcgcct gtatcaatca gtttatatgc ttcatgcata ttgttcacaa tcttattctt
     7321 gtttagaaga ataaccaatt tttcggcaat aaaataaatg gcacgctgct ttacatcctg
     7381 ttctatccat accgcaccat tctccgcaac cccaatcttt ccatctataa cggccaaatc
     7441 cgtcccattc agttctgcag ggtcttccac atcatcggga ttaaatgtcg cacatgtaat
     7501 agtcttcaga ttagaagcga tgcgtttggc tccaggataa gcctttttaa tgaaatcatt
     7561 cacgtcctcc tctttctcca aaagaatggc ttcccctcct acctgcttca tgatgagata
     7621 aaactgttcc accttactgg gataagttaa tgccatatct tccaatggct ttaaatccgg
     7681 tttctcatat tttacatgtg tagcacgccg gatgctctgc aatatatctt ctctgctgct
     7741 cataatttac ttcactttac ctttcttcca catttcattg aaactctcct tagcaaattt
     7801 cggcatctca cgcccttttc cccagtcatt caatccgtta tagataagaa aacgtggcat
     7861 tccgtttacc aatggcgccc attttaatgc catattgaat atcaaagggc gattcatcat
     7921 gaattccatg ccaccggaca ttaccctttt catccggtct gccttaccca atttatccaa
     7981 atcctgacgc cacagataga tctgctccgc caaatccacc ttagccggac acacaaacga
     8041 acaggaataa cacaaagaac aagccgacac attgtcataa taatgcaacg gcgccttcaa
     8101 cattcccagg ttaatcccga tagggccggg aataaaataa gtataagagt accccccac
//
