LOCUS       gnlProkkaLDEMOHEN_95   14314 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LDEMOHEN_95.
ACCESSION   gnlProkkaLDEMOHEN_95
VERSION     gnlProkkaLDEMOHEN_95
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 18:36:24
            Original ID  :: gnl|Prokka|LDEMOHEN_95
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 4328
            Orig. end    :: 18642
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14314
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:9314]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..9314
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..14314
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..14314
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             complement(426..632)
                     /locus_tag="ctg95_11"
                     /transl_table=11
                     /translation="MYEDHHRKQDGGDQLATVAPLVEFLLNGNEILDTHLVQMLVNPEK
                     PDVPLNKNDAFQKVQKRRVQEKR"
     CDS             651..968
                     /locus_tag="ctg95_12"
                     /transl_table=11
                     /translation="MEVLSFSTVSRYMVMRGESSPAVIPDEQMARFRFMLDYSEESISM
                     NSAPLARGEKVRVIKGPLTGLVGELVNVDGKSKIAVRLNMLGCACVDMPVGYVEPIMAA
                     V"
     CDS             979..1347
                     /locus_tag="ctg95_13"
                     /transl_table=11
                     /translation="MDAVILQENIEGLLNLVRMLLPGGGSAGCVYLDDLSVLQRSIHKQ
                     INDLYSQRGKTPEQDATLCLAILQGYNVSMYANPEDEDRKRSVLQRSLTLLDVLPPSLL
                     KQQLSAVCHGMQELCETN"
     CDS             complement(1447..2109)
                     /locus_tag="ctg95_14"
                     /transl_table=11
                     /translation="MEDGREGRKGVGTRLHDLSHHVDLDSTDITQSQTDVGSRVGSIVQ
                     TVINLIQGALQIVVGGGDCHTAKVDRPDLLNIDGAFRRDLLADRVLASTPDIDDDFITG
                     TQTVIGRSGKVDTRLERQVAGIEDVAPENLILTGQIFRSRMILQHVGSILLSLFTKAGL
                     VDIRTRGLGGVYIVLLLIELLHTHSLFLGHTFTRKFGSKLFLFLALPDAVLYIFQYL"
     CDS             2131..3774
                     /gene_functions="transport (smcogs)
                     SMCOG1186:polysaccharide export protein (Score: 70.9;
                     E-value: 1.3e-21)"
                     /gene_kind="transport"
                     /locus_tag="ctg95_15"
                     /transl_table=11
                     /translation="MSDIGSLRNVQLVRNGKNIATIDVYEFIMKGNTQDDIRLQEGDVV
                     IVPAYDVLVKISGKVKRPMRFEMKKDENLATLIKYAGGFEADAYTRSLRVVRQNGEEYE
                     VNTVKDMDYSIYTMRNGDVVTAEAILNRFTNKLEIRGAVYRPGIYQLSGKLNTIRELVH
                     EAQGLTGDAFLNRAVLYRQREDLTSEVVQIDIKSIMNGTSPNLALMKNDILYIPSIHDL
                     EDRGNVTVHGEVAHPDSYPYADNMTLEDLIIQAGGLKEAASTVRIDVSRRIKNPRSTAD
                     NDTIGQMYTFSLKDGFVIDGQPGFILQPYDEVYVRRSPGYQAQQNVVIDGEILFGGNYA
                     MTNREERLSDLVNKAGGPTSLAYLRGAKLTRVASAGEKKRMGDVIRLMSRQLGEAMIDS
                     LGIGVEDTFTVGIDLEKALTNPKGSADLVLREGDVVFIPKNTNTVTINGAVMVPNTVSY
                     MKGKDVDYYLNQAGGYSDNARKSKKFIVYMNGQVTKVKGSGKKQIEPGCEIIVPSKTKK
                     KGNIANILGYATSFSSLGMMIASIANLIKK"
     CDS             3789..4937
                     /locus_tag="ctg95_16"
                     /transl_table=11
                     /translation="MSEQRQDKNLLSEPHKTESDEIEIDLVDIFRKIIAIRKKLYKAVG
                     IGFIIGIIVAVSIPKQYTVKVTLSPEMGNAKGSNGLAGLAASFLGDGALIGESTDALNA
                     SLSADIVSSTPFLLELLEMEVSVSKKDDKMTLGSYLDEESSPWWDYLIGFPGIVIGGVK
                     SLFGEDTVPASGGRQGTIELTKEVNEKINFLKKKISASIDKKTAITSITVTLQNPQITA
                     VIADSVVHKLQEYIIGYRTSKVKEDCAYLERLFKERQQEYYAAQRKYADYVDAHDNVVL
                     QSVRAEQERLQNDMSLAYQIYSQVANQLQVSRAKVQEEKPVFAVVEPAVVPLKPSGMGL
                     KIYILLFVFLSVSGTLVWVLFGKSLLASLRKELKNKEIEQDK"
     CDS             5001..5720
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 56.8; E-value:
                     3.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg95_17"
                     /sec_met_domain="NTP_transf_3 (E-value: 9.1e-17, bitscore:
                     52.9, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRTAMIMAAGMGTRFGQYTELIPKGFVKVGGIPMIVRSIDTLLS
                     CGIERIVIGTGYKQEVYEELKTDYPMLETCFSPRYAETNSMYTLYNTREILGNDDFLLL
                     ESDLIFEKQAIMSLLECPAADAMLIAPVTKFQDQYYVEHDDNFRLSSCSVNKNKLNAKG
                     ELVGIHKLSGSFYKIMCADYASIVDEQPNLGYEYELLRISCSQSPVYVHKVEGLKWYEI
                     DDISDLEYAEKYIVPYC"
     CDS             5741..7042
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 209;
                     E-value: 1.4e-63)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg95_18"
                     /sec_met_domain="CTP_transf_like (E-value: 4.9e-13,
                     bitscore: 40.6, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 2.1e-121, bitscore:
                     395.5, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 2.6e-63, bitscore:
                     204.8, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKTVYIGMTADIMHPGLIRIINEATKYGDVIIGLLTDKAIAEHK
                     RLPYLTYEQRKEVVQNIKGVCKVVPQEEWSYVENLKRIKPDYIIHGDDWKTGPLREERV
                     RVFEVMNEQGGKVIEIPYTLGINSSSLDKDIKAIGTTPDVRLKSLRRLINAKPVVRILE
                     AHDGLCGLIIENQEILKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAIIIEDKVGLKKNSLFGTDVIQTQDT
                     IEGFCNKIKAGKASQITDDFMIIARIESLIAGKPVSDALERAFAYVQAGADGIMIHSKN
                     KSGEDIKEFCLAFRKQYAHVPIVVVPTTYDHIYESELCDWGVNIIIYANHMLRAAYPAM
                     MNVAKTILENERALEVRELCMPIKEILELIPGTK"
     CDS             7059..8183
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 58.1;
                     E-value: 1.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg95_19"
                     /sec_met_domain="TPP_enzyme_N (E-value: 2.7e-20, bitscore:
                     63.7, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.3e-20, bitscore:
                     64.0, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSVEKVYETFLSHDVDFFTGVPDSLLKNICAYITDHTSREKHII
                     AANEGAAVGIASGYYMASGKVPVVYMQNSGLGNTVNPLLSLADEQVYSFPMLLMIGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMRIPYIILDSNENDAFIQLHDIIQSAKTKNIPHAII
                     IRKDVFGKYKLQKEFGCNYPLSREDALQIVVDYLPENSVVVSTTGKLSRELFEYREMKE
                     QGHEHDFLTVGSMGHSSSISLGIAIAKQDRPVYCLDGDGAFIMHLGAISNIGDLSPKNY
                     YHILFNNGAHESVGGQPTLGFSLDIPAIVRGSGYKHTYTVCTKVEIEEAMKQLPKLCGP
                     VLLEIKVKIDSRENLGRPTTTPIENKEHFMDFLK"
     CDS             8193..9314
                     /NRPS_PKS="Domain: Aminotran_5 (30-366). E-value: 2.9e-34.
                     Score: 110.4. Matches aSDomain:
                     nrpspksdomains_ctg95_20_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 76.3; E-value:
                     3.6e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg95_20"
                     /sec_met_domain="Aminotran_5 (E-value: 3.8e-31, bitscore:
                     99.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIERKILLNPGPATTTDSVKMAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGNLEKHTAVLFCGSGTINIDICLNSLLPEDKRVLIVNNGAYSTRAVEVCQYY
                     GLPHINLEFSVYERPDLSVVENVLQENPDIAMVYTTHHETGTGILNPIREMGAIAHKYQ
                     AKFVVDTTSSLGMIPFDIEKDNVDFCMASAQKGLQAMTGLSFIIGNEEQIKFSKKYPKR
                     SYYCNLYLQYENFERTGEMHFTPPVQTIYATRQALNEYFEVGEEAKFARHKRVFEAIHS
                     GINEIGLKSVIKREWQSGLVVSVQYPEDPNWDFEKIHDYCYERGFTIYPGKISTEDTFR
                     ICALGEIEVDDIENFFKVLKAALNHYNVTLPMS"
     aSDomain        8283..9290
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg95_20_Aminotran_5.1"
                     /evalue="2.90E-34"
                     /label="ctg95_20_Aminotran_5.1"
                     /locus_tag="ctg95_20"
                     /protein_end="366"
                     /protein_start="30"
                     /score="110.4"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGNLEKHTAVLFCGSGTINIDICL
                     NSLLPEDKRVLIVNNGAYSTRAVEVCQYYGLPHINLEFSVYERPDLSVVENVLQENPDI
                     AMVYTTHHETGTGILNPIREMGAIAHKYQAKFVVDTTSSLGMIPFDIEKDNVDFCMASA
                     QKGLQAMTGLSFIIGNEEQIKFSKKYPKRSYYCNLYLQYENFERTGEMHFTPPVQTIYA
                     TRQALNEYFEVGEEAKFARHKRVFEAIHSGINEIGLKSVIKREWQSGLVVSVQYPEDPN
                     WDFEKIHDYCYERGFTIYPGKISTEDTFRICALGEIEVDDIENFFKVLKAALNHY"
     CDS             9311..9454
                     /locus_tag="ctg95_21"
                     /transl_table=11
                     /translation="MIAEILLKIRHFYFSLRGIHFHPESVISGVPIRSFRLVGKYGNIN
                     LR"
     CDS             9657..11141
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg95_22"
                     /sec_met_domain="Polysacc_synt (E-value: 3.4e-31, bitscore:
                     99.7, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENLKQKTVSGIMWSAIERFSLQGVQFIIQIILARLLLPSDYGMI
                     GMLAIFLQVAQVFIDSGFTNALIQKKDRTEEDFATVFYFNILVAVLFYGILFFSASLIA
                     DFYNMPTLVLVIRFIALSLIINALSAIHRTKLIISVNFKTQSKISLGAAFISGVIGIWM
                     AYVGCGVWALVWQTLLNSIILTILFYCLVHWKPLHTFSKSSFERLFNFGSKLLISSLIN
                     TVYRNLYTIVIGKKFSVVELGYYTRAEQFAIFPSNNLNALVSRVAYPILSSIQDDDKCL
                     TNAYRNYIRLSSYIIFPLMVGLLVLANPIISLLLTDKWSGVVILLQILCLDWMFDHLSQ
                     INLNLLWVKGRSDLSLRLEIIKKTIAIFILFVSIPFGLEVMCWGRVIYSLIATYLNTYY
                     TNSLIDLTLKLQVKDVFPSLILSFIMGGIVFICISFFETDIVKIVIGCITGMSFYILLS
                     FLFKLDSFFCILTLIIKTKNIDENIKKDFSSNSKDN"
     CDS             11095..12354
                     /locus_tag="ctg95_23"
                     /transl_table=11
                     /translation="MKILKKIFQVIVKIINHCVPVDSRSLYFIPHPNCRTDKYDIINYS
                     SDNVLVLLNYLLKKDSAEYYKIYVEIYDSSRISEYQEYIYGINPNIRCTFLCACVGRFF
                     FNKVSFKDMLYAFFCFCRASKCFTATFYYDFSFKKRSQQIICLGYYTPFKDDYHMGDHS
                     YDKFRNTTCNSFDYSIATSCLSARIISIDCGISYDKFKVLGFPRNDLLISKNNCNVIKR
                     EISKFAGYDVTKYIVYTPTFRDYETVTEGNLRSILGYVDCDLLKLSKILLKFNAALILK
                     LHPLQNKTVLKKDLPKGILVFEQTYKYGLYDLLSFSDGMITDYTSTYFDFLLVNKPVIF
                     NFYDIEEYRRVRGFSFEPIEFFCAGDIVYNYNELIDAVMGLLAGKDIHAEHRRHISLLM
                     NQFQDDNSTKRICDIFLNEI"
     CDS             12363..13112
                     /locus_tag="ctg95_24"
                     /transl_table=11
                     /translation="MNYAFVVQARLGSTRLPGKILKPFYGNQSILDLMVHKLSAISNIP
                     VIIATTNSVINEPIEKKALALGVKCFRGEENDVLKRFIDVAEYFDIQGIFRICSDNPFL
                     DVHAARQLVEIAMKSCNDYISFDIDGTPSIKTHFGFWGEFVTLDALKRVIGFTDDLLYR
                     EHVTNYIYSHPELFNIQWISGSPVVSKHHNIRLTIDTLEDFSVAQRIYRDLQEKRVEIS
                     IEVIVDYVRRHAEYIQLMKQQIKKNSK"
     CDS             13112..14152
                     /locus_tag="ctg95_25"
                     /transl_table=11
                     /translation="MKNTYIIGEIGQNHNGSVDLAKLIIELISRPVHEDVFGMDFMPMN
                     AVKMTKRDLSEELAVSQMNQIYDNPNSFGRTYGEHRAFLELDDQAHFEIYKYAKSLGLD
                     FIETLCAKGCLSLLKLFTPDRLKVASRDLTNLPLLEALAETHIPIILSTGMAGQRELDN
                     ALDVITRYHSNIAILHCVSQYPTHPNNLNLRTITYLKKHYNKFEIGFSDHTIGISAATA
                     AVAMGAEIIEKHVTIDRHMKGTDQLGSLGPDGVNRMIRDIRIAECWLGTEDLYIEKGVE
                     KSKIKLERSIATRKYIPAGSIIQESDIHLLSPGDGYKWIDKKNVIGHFAKQDISANEII
                     YPDFIE"
ORIGIN
        1 tgttcctggt tgatccgccg tacctttcca ccgaggtggg tacttacgag atgtcctggc
       61 gtctggctga ctacctggac gtgctgaccg ttctgaaagg gcattcgttt gtgtacttca
      121 cttcgaacaa gtcctccatt ttagaactgt gcgactggat ggaccgaaac ccatttgtcg
      181 gcagcccatt caaggaatgc aggaaagcgg agtttagtgc aagcgtaaac tatcaagcta
      241 aatatacaga catgatgctg tacacgaagc cggatgaagt gtcaggtata gcagcctaac
      301 attgcataaa gatagggaat tattttgaat ctgcaatggc ttttaaatga tattttaaag
      361 ccatttaaag agggttcaag tgaaagaaaa acggtgggct ttgatcatgc tgaataggac
      421 cgcgctcacc ttttttcttg tacgcgtcgt ttttgtactt tttgaaacgc atcgtttttg
      481 ttaagcggca cgtctggttt ttccggattt acgagcatct ggacaagatg ggtatcgaga
      541 atttcgttcc cgttcaacag gaattccacc aatggagcga ccgtcgcaag ctggtcacct
      601 ccgtcttgct tcctatgatg gtcttcgtac acgttgaccc tagggaacgc atggaagtct
      661 tgagtttttc tactgtgagc cgttacatgg tgatgcgcgg cgagagtagt cctgccgtaa
      721 tccctgacga gcagatggcc cgtttccgtt tcatgcttga ttattcagaa gaatcgataa
      781 gcatgaacag tgcccctttg gcccgtggtg aaaaggtccg cgtgattaaa ggccccctga
      841 cggggcttgt aggtgaactg gttaatgtgg atggcaagag taagatcgcc gttcgcctga
      901 atatgttggg atgtgcctgt gtggatatgc ctgtcggtta tgtagagcct attatggcgg
      961 ctgtttaagg cgagggatat ggatgctgtt attttacagg aaaacataga gggtttatta
     1021 aatttggtac gcatgctgct tcctggtggg gggagtgcag gctgcgtgta tctggatgac
     1081 ctttccgtct tacaacgatc catccacaaa cagataaacg atttatattc acaacggggg
     1141 aagacaccgg agcaggatgc taccctgtgt ttggccatcc ttcagggata taatgtgtcg
     1201 atgtacgcca atccggaaga cgaggataga aaacggtctg tcttgcagcg ttctctgacc
     1261 ctccttgatg ttcttccccc ttctcttctt aaacaacagt tgtctgcggt ctgtcacggt
     1321 atgcaggaac tgtgtgaaac caactaactt ttttatttgt caatgaaaat acaacgactt
     1381 tttagcattc tacttttttg ttctatgctg ggaagtggct cgcttatggc ccagtccatg
     1441 agtgactcac aggtactgga atatgtaaag gacggcatcc ggcaaggcaa ggaacagaaa
     1501 cagcttgctt ccgaacttgc gcgtaaaggt gtgaccaagg aacaggctat gcgtgtgaag
     1561 cagctctatg agcagcagaa caatgtaaac acctcccaat ccacgggtac ggatatcaac
     1621 gagtcccgcc ttcgtgaaga gactaaggag aatacttccg acatgctgga ggatcatccg
     1681 actacggaag atttggcccg tgaggatcag gttttcgggc gcaacatctt caatacccgc
     1741 aacctgacgt tcgagcctag tgtcaacctt gccactcctg ccaattaccg tctgggtccc
     1801 ggtgatgaag tcatcatcga tatctggggt gctagccaga acacgatccg ccagcagatc
     1861 tctcctgaag gcaccatcaa tattcagaag atcgggcctg tcaaccttag cggtatgaca
     1921 gtctccgccg ccaacgacta tctgaagggc gccctgaata agatttataa cggtctgaac
     1981 aatactaccg accctacttc cgacatccgt ctgactttgg gtaatatccg tactatccag
     2041 atcaacgtga tgggagaggt cgtgcagccg ggtacctacg ccctttcttc cttctctacc
     2101 gtcttccacg cgctttaccg tgcaggcggc gtgagtgata tcggcagcct ccgaaacgtt
     2161 cagttggtac gcaacggaaa gaatatcgcc accatcgatg tttacgaatt tatcatgaaa
     2221 ggcaacacac aggatgacat ccgccttcag gaaggtgacg tggttatcgt ccccgcctac
     2281 gatgtcttgg taaagatcag cggcaaggta aaacgtccta tgcgtttcga aatgaagaaa
     2341 gacgagaacc ttgccacttt aatcaaatat gccggcggct ttgaagccga tgcctacacc
     2401 cgttcattgc gtgttgtccg tcagaatggt gaggaatatg aggtcaacac agtcaaggat
     2461 atggactaca gcatctacac aatgcgtaac ggtgacgttg taacagccga agccatcctt
     2521 aaccgcttca caaacaaact ggaaatccgt ggcgctgtct atcgtcccgg catctaccag
     2581 ctcagcggta aactgaatac gatccgcgaa cttgtccatg aagcccaggg cctgaccggt
     2641 gacgctttcc tgaatcgtgc cgtcctttat cgccagcgtg aggatttgac cagtgaagtt
     2701 gttcagatag acatcaagtc tatcatgaac ggtacttccc cgaaccttgc cctgatgaag
     2761 aatgacatcc tttacattcc gagcattcac gatctggaag atcgtggcaa tgtcacagtt
     2821 cacggtgaag tcgcgcaccc cgattcctat ccctatgctg ataacatgac acttgaggat
     2881 ctgatcatcc aggccggtgg cttgaaagaa gccgcctcta ctgtccgcat tgatgtgtcg
     2941 agacgaatca agaatccccg tagtaccgcg gataatgata ctatcggtca gatgtatacc
     3001 ttctctttaa aggatggttt tgtcatcgac ggtcagcccg gttttatcct tcagccttat
     3061 gacgaagttt atgtccgtcg cagtccgggt tatcaggcgc aacagaatgt agtcattgat
     3121 ggtgaaattc tttttggagg aaattatgcc atgaccaacc gtgaggaacg tctttccgat
     3181 ctggtgaata aagcgggtgg tcctaccagt cttgcctatc tccgtggtgc gaaattgacc
     3241 cgtgtggcaa gtgccggtga gaagaaacgt atgggtgatg tgattcgcct gatgagcagg
     3301 caattgggtg aagccatgat tgactctttg ggtattggtg ttgaggatac ctttacggta
     3361 ggcattgatc tggagaaggc tttgactaat cctaagggta gtgccgacct tgtcctacgt
     3421 gaaggtgatg tggtctttat acctaagaat actaatactg taactatcaa tggtgctgtc
     3481 atggtcccca atacggtttc ttatatgaag ggaaaggatg tggattatta cctgaatcag
     3541 gccggtggct attctgataa tgcaaggaag agtaagaagt tcattgttta catgaatggt
     3601 caggtgacta aggttaaggg tagtggtaag aaacagattg agccggggtg tgagattatt
     3661 gtccctagta agactaagaa gaaagggaac atagctaata ttttaggtta tgctacttcc
     3721 ttcagttctt tgggtatgat gattgcttct attgctaatc ttataaagaa ataatagaat
     3781 ttacttttat gtcagaacaa cgacaggata aaaacttgct ctctgaacca cacaagacag
     3841 aaagtgatga gatagaaatt gatttggtcg atatctttcg caagattatt gctattcgta
     3901 aaaaattata caaagccgtt ggaattggtt ttattattgg aataattgta gcggtaagta
     3961 tccctaaaca gtatacagta aaggttaccc tttctcctga aatgggtaat gcgaagggga
     4021 gtaatggttt agccggactt gctgcctcgt ttttaggaga tggggctcta ataggggaaa
     4081 gcacagatgc cttgaacgct tctctttcag cagatattgt ttcttcaact ccttttttat
     4141 tggaactttt agaaatggaa gtctccgttt ctaaaaagga tgataaaatg actttggggt
     4201 cttatctcga tgaagagtca tctccttggt gggattatct tataggtttt ccgggcatag
     4261 taataggagg agttaaatca ttattcggag aagatacggt tcctgccagt ggagggcggc
     4321 aaggaactat cgaattgacg aaagaagtaa atgagaagat taattttctt aaaaagaaga
     4381 ttagtgcatc cattgataag aaaaccgcca taacgagcat tacggtcacc ttacaaaatc
     4441 cgcagatcac tgcggttata gcagattctg tcgttcataa attacaggaa tatattattg
     4501 ggtatcgaac ttcaaaagta aaagaagact gtgcgtattt ggaacggttg tttaaagaac
     4561 gtcagcagga atattatgct gctcaaagaa aatatgcgga ttatgtagac gcccatgata
     4621 atgtggttct tcaaagtgta cgtgctgagc aggagcgttt acagaacgac atgagtttgg
     4681 cttatcagat atatagtcag gttgctaatc agttgcaggt atccagagcc aaagtacagg
     4741 aggaaaagcc agtctttgca gtagttgagc ctgccgttgt acctttgaaa ccttcaggta
     4801 tgggactcaa aatttatatt ttattatttg tgttcctgtc cgtatctggt actctcgtgt
     4861 gggtgctttt tggcaaaagt ctgctggcta gcttgagaaa agaactgaaa aataaggaga
     4921 tagaacagga taaatgatgc taaaacgtcg tttacaacat tcttatatca attaaattca
     4981 cttactatat tattaaacga atgatacgta cagctatgat aatggctgcg ggcatgggaa
     5041 cccgttttgg ccaatataca gaattgatac ctaaagggtt cgtcaaagtg ggaggtattc
     5101 caatgatagt tcgttctatt gatactttgt tatcatgtgg cattgagcga attgtaatcg
     5161 gtacaggata taaacaagaa gtgtatgaag aattgaagac agactatcct atgcttgaaa
     5221 cctgttttag tcctcgttat gctgaaacaa atagtatgta tactttatat aatacgcggg
     5281 agattctggg caatgatgat tttttattgt tagagtcaga tttgattttt gagaaacaag
     5341 caataatgag cttattggag tgtcctgcgg cagatgcaat gctaatagct ccagtgacaa
     5401 agtttcaaga tcagtattat gtggaacatg atgataattt tcgcctttct tcatgttctg
     5461 tcaataagaa taaactaaac gctaaaggcg aattggtcgg catccataaa ctatccggtt
     5521 ccttttacaa aataatgtgt gctgattatg cgagtattgt tgatgaacaa cccaatttgg
     5581 gatatgagta tgaattacta cgaatatctt gttctcaatc tcctgtttat gtgcataaag
     5641 tagaagggct gaaatggtat gaaatcgatg acatatcaga tttggaatat gcagagaaat
     5701 atattgttcc gtattgttaa ttaaaataat atatcaaatt atgagtaaga cagtttatat
     5761 tgggatgact gcggatatta tgcatccagg acttatccgc attattaatg aggctactaa
     5821 atacggtgat gttataattg gtcttcttac agataaagct attgcggagc ataaacgcct
     5881 tccttatctg acctatgaac aacgaaaaga ggtagttcag aatattaaag gcgtatgtaa
     5941 agttgttcct caagaagaat ggagctatgt tgagaatctc aaacgcatta aacctgacta
     6001 tataattcat ggtgacgact ggaaaaccgg gcctttacgt gaagaacgtg tgcgcgtatt
     6061 tgaagtgatg aatgaacagg gaggaaaggt tatcgaaatt ccatatactt tgggaattaa
     6121 ttcatcttct ttggataaag atattaaggc tataggtaca acccccgatg tacgtttaaa
     6181 atcacttcgt cgtttgataa atgccaaacc cgtagttcgt attttggaag ctcatgatgg
     6241 actatgtggc ttgataattg aaaatcagga aatattaaaa ggggataaga gggaagtatt
     6301 tgacggaatg tggtctagta gccttacgga ttcaaccagt aaaggtaaac ctgatattga
     6361 ggcggttgat ttaactaccc gattgcaaga tttgaataat attctggagt gtactactaa
     6421 acctattatt tttgatggag atacaggtgg taaaattgaa catttcgtat ttacagtacg
     6481 cactttagaa cgtcatggaa tctcagctat aataattgaa gataaagttg gattaaaaaa
     6541 gaattcattg tttggtacgg atgttattca aactcaagat acaatcgaag gtttctgtaa
     6601 taagatcaaa gcaggaaagg catctcagat tactgatgat tttatgatta tcgctcgtat
     6661 tgagagcctt atagcgggaa agccggtaag tgatgcttta gagagagctt ttgcatatgt
     6721 ccaagcggga gcggacggta ttatgataca tagcaaaaat aagtcgggtg aagatataaa
     6781 agaattttgt ttggcgtttc gaaaacagta tgctcatgta cctatcgtgg ttgtgcctac
     6841 tacatatgat catatttatg agtctgaatt gtgtgattgg ggtgtgaata taattattta
     6901 tgctaatcat atgcttcgtg ccgcttatcc tgctatgatg aatgtcgcaa agacaatatt
     6961 agagaatgaa cgtgctttag aggtgcgcga actgtgtatg cccataaagg aaatattgga
     7021 actgattccg ggtactaaat aaaaagagga atatcattat gttaagcgta gaaaaagttt
     7081 atgaaacgtt tctgagtcat gacgttgatt tttttacagg agttccagac tcattactaa
     7141 aaaatatttg tgcctatatc acagaccata cttcacgtga gaaacatatc atagctgcta
     7201 atgaaggtgc ggctgtagga attgcttcag gttattatat ggcatcaggt aaggttcctg
     7261 tggtttatat gcaaaattcc ggcttgggta atactgttaa tcctctgctg tctcttgcag
     7321 atgaacaagt atacagtttt ccaatgcttt tgatgattgg ctggcgtggt gaaccgggaa
     7381 caaaagatga acctcagcat aaaaaacagg gtgaggtaac tttagatttg ttgaaagcaa
     7441 tgcggattcc ttatataatt cttgattcaa atgagaatga tgcatttatt cagctccatg
     7501 atatcataca atcagcaaaa actaagaata ttcctcatgc aataattatt cggaaagatg
     7561 tttttggcaa atataaactt caaaaagaat tcggatgtaa ttatccttta tctcgtgagg
     7621 atgctttgca aatagttgtg gattatctgc ctgaaaattc agtggtcgtt tctactactg
     7681 gcaagctttc tcgtgagtta tttgaatatc gagaaatgaa agagcaaggt catgaacatg
     7741 attttctcac ggtaggctct atgggacata gttcatcaat ctctttgggt attgctatag
     7801 caaagcaaga tagacctgtt tattgcttgg atggtgatgg agcatttatt atgcatttag
     7861 gggcaattag taacattggc gatttatctc ctaaaaacta ttatcatatt ttgtttaaca
     7921 atggtgctca cgagtctgta ggaggacagc ctactttagg atttagttta gatattccgg
     7981 ctattgtccg ggggagcgga tataagcata cttatactgt ttgtacaaaa gttgaaattg
     8041 aagaagcaat gaagcagctt ccaaagttat gtggtcctgt tttgttggaa ataaaggtta
     8101 aaatagattc tcgggagaat ttgggacgtc ccacgactac tcctatagag aataaggagc
     8161 attttatgga ttttttaaaa taaatatatt cgatgaaaat agaaagaaaa atattattaa
     8221 atcctgggcc tgccactact actgacagtg taaaaatggc acaagttgtt ccggatattt
     8281 gtcctcgcga gaaagaattc gcaggtatga tgaaacaact tcgggacgat ttggtcagag
     8341 ttgctcatgg gaatttggaa aaacataccg ctgtattatt ttgtggctca ggaaccatca
     8401 atattgatat atgtctaaat tctcttcttc ctgaagacaa aagggtgctt atagtgaata
     8461 atggggcata tagtacccgt gctgtagaag tgtgtcaata ttatggtttg cctcatatta
     8521 atttggaatt ttccgtttac gaacgtcctg atttatcggt ggtggaaaat gtgttgcaag
     8581 aaaaccctga tatagctatg gtttatacta ctcatcacga gacgggtaca ggaattctga
     8641 accctattcg cgagatgggc gctatagctc ataaatatca ggctaaattt gtcgtcgata
     8701 ctacttcttc tttgggcatg attccttttg atatagagaa agataatgtg gatttttgta
     8761 tggcatctgc tcaaaaagga cttcaagcga tgacaggtct ttcttttatt ataggcaatg
     8821 aggaacagat taagttttca aaaaaatatc ccaagcgttc ttactattgt aatctttatt
     8881 tgcagtatga gaattttgag agaacaggtg aaatgcattt tactccaccg gtacaaacta
     8941 tttacgccac tcgtcaggca ttaaatgaat attttgaagt gggcgaagaa gctaagtttg
     9001 cccgtcacaa aagagttttt gaggctattc attcaggtat aaatgaaatt ggtcttaaaa
     9061 gtgtaataaa gcgtgaatgg cagtcaggac tggttgtctc agttcaatac ccagaagatc
     9121 ccaactggga ttttgagaaa atacatgatt attgttatga gcgtggattt acgatttatc
     9181 caggtaagat atcaactgag gatactttta gaatctgtgc tttaggagag atagaagtag
     9241 atgatattga gaacttcttt aaagtactaa aagctgcatt aaatcattat aacgtcacat
     9301 taccgatgtc atgattgcag aaatattatt gaagattcgt catttttatt tttcccttcg
     9361 tggtatacac ttccatccgg aaagtgttat ttctggagtg cctattcgta gctttcgctt
     9421 agtaggcaaa tatggcaata ttaatttgag gtgatgcatt aggagttctt tattcttata
     9481 aaaaggcatc ggtagttata ggagataatg tttgtgtagg gtaatttgct tttagtggga
     9541 gagaccatat tagaattgcg gtaaggggcg aagaagataa taaccaatta gtgagaatat
     9601 tggaaaaata taattgatat cggtaaatgt agttgttttt atactgtatt taagtaatgg
     9661 aaaatctaaa gcagaaaact gtatcaggaa taatgtggag cgctatagaa cgtttctctt
     9721 tacaaggagt acagtttata attcaaataa tattagctcg tttgttacta ccatcggatt
     9781 atggtatgat cgggatgtta gctatttttc ttcaagtcgc tcaagtattt atagatagcg
     9841 gttttacaaa tgctttaatc cagaaaaaag atagaactga agaggatttt gccactgtct
     9901 tttattttaa tattcttgtc gcagtattat tctatggtat attgtttttt tcggcttctt
     9961 tgattgcaga cttttataat atgccgactt tagttcttgt aattcgtttt atagcattgt
    10021 cgttgataat aaatgcttta tcggctattc acagaacgaa attaattatt tctgtaaact
    10081 ttaaaactca atctaaaatt tcattaggtg cagcttttat atcaggcgtg attggaatat
    10141 ggatggctta tgtcggttgc ggggtttggg ctttagtttg gcaaactctc ttaaatagta
    10201 taattttgac aattttgttt tactgtttag ttcattggaa acctttacat actttctcca
    10261 aaagttcttt tgaaagatta ttcaatttcg gatctaaatt attgatctca agtcttatca
    10321 atacagtata tcgtaatctg tataccattg taataggaaa aaagttttct gttgtagagc
    10381 ttggttacta tacaagagca gaacaatttg ctatttttcc ttccaataat ttaaatgctt
    10441 tagtttctag agtagcgtat cctattttaa gttccattca agatgatgat aaatgtttaa
    10501 caaacgcata tcgaaattat atcagattat cctcatatat aatttttcct ttaatggtag
    10561 gtttacttgt tttggctaat cctattatta gtctattgtt aacagataaa tggtctgggg
    10621 ttgttattct tttacagata ttatgtttgg attggatgtt tgatcatttg agccaaatta
    10681 atttgaattt attatgggtt aaaggaagat ctgatctgtc gttacgtctt gaaataataa
    10741 aaaagacgat tgctatattc attctttttg tgtcaattcc atttgggctt gaggtgatgt
    10801 gttggggtag agttatttac tctctgatag cgacatattt gaatacatat tatactaatt
    10861 ctcttattga tttgacctta aagttgcaag ttaaagatgt atttccaagt ttaatactct
    10921 ctttcataat gggggggatc gtttttatat gtatttcttt ctttgaaact gatatagtaa
    10981 aaattgtgat tggatgtatt actgggatgt ccttctatat tttactttct tttcttttta
    11041 agctagattc ttttttttgt attttaacat tgatcattaa aactaaaaac atagatgaaa
    11101 atattaaaaa agatttttca agtaatagta aagataatta accattgtgt gccagttgat
    11161 agtagatcat tgtattttat tcctcatcct aattgtagaa cagataaata tgatattata
    11221 aattattcgt cagataatgt tctcgttctg ttaaattatt tgctaaaaaa agatagtgct
    11281 gaatattata aaatatatgt tgaaatttat gattcttctc gtatttcaga atatcaagaa
    11341 tatatttatg gcataaatcc taatattagg tgtacttttt tatgtgcatg tgttggacgt
    11401 tttttcttta ataaagtatc ttttaaggat atgttgtatg cctttttttg tttttgtaga
    11461 gcaagtaaat gttttacagc tactttttat tatgattttt catttaaaaa acgctctcaa
    11521 cagattattt gtttggggta ttatactcca ttcaaagatg attatcatat gggagatcat
    11581 tcttatgata aatttcgtaa tacaacatgt aactcatttg attactctat tgctacttct
    11641 tgtttaagtg ctagaataat ttcaatagat tgtggaatct catatgataa attcaaagtc
    11701 ttaggatttc ccagaaatga tttgttgata tcgaaaaata attgcaatgt tattaaaaga
    11761 gaaatttcga aatttgcggg atatgatgta actaaatata tagtttatac tccaactttt
    11821 cgtgattatg agactgttac tgaaggtaat ttgcgttcaa tactcggata tgttgattgt
    11881 gatttgttga agttatcgaa aatactattg aagtttaatg cggctttaat tcttaaatta
    11941 catcctttgc agaataaaac agtcttaaaa aaagatttac ccaaaggtat acttgttttt
    12001 gaacaaacat ataaatatgg attgtatgat cttttgtcat tttcagatgg aatgattact
    12061 gattatactt ctacctattt tgatttttta ttggtaaata agccagtcat atttaatttc
    12121 tacgatattg aagaatatcg tagagtaaga ggtttctcat ttgagccaat agaatttttt
    12181 tgtgcaggtg atattgttta caattataat gaattgatag atgctgtgat gggacttctg
    12241 gctgggaaag atattcatgc tgaacatcga cgtcatattt ctttattgat gaatcaattt
    12301 caagatgata attcaacaaa gagaatatgt gacatatttt taaatgaaat ataataaata
    12361 aaatgaatta tgcatttgtg gtgcaggcac gtttaggttc tacacgtttg cctggaaaaa
    12421 tattgaaacc tttctatggt aatcaatcga ttcttgactt aatggttcat aagctttctg
    12481 ctatatcgaa tattccagta attattgcta caacaaattc tgttatcaat gaacctattg
    12541 aaaagaaggc acttgcatta ggggtaaagt gcttcagagg tgaggagaat gatgttttaa
    12601 aacgattcat tgatgttgct gaatattttg atatacaggg tatattccga atatgttctg
    12661 ataatccatt tttagatgta catgcggcaa ggcaattggt cgaaatagca atgaaatctt
    12721 gtaacgatta tataagtttt gatattgatg gtactccatc aattaaaact catttcggtt
    12781 tttggggaga atttgtaacg cttgatgcgc tgaagcgagt tataggattt actgatgatt
    12841 tattgtatcg tgaacatgtt actaattata tatatagtca tcctgaactt tttaatattc
    12901 aatggatttc aggatctcca gtcgtgtcaa aacatcataa tattcgactc acgattgata
    12961 cgttagaaga tttttctgta gcacagagaa tatataggga tttacaagaa aaaagagtgg
    13021 aaatatcgat agaagtgatt gtagactatg ttcgaagaca tgcagaatac attcaactga
    13081 tgaaacaaca aataaaaaaa aattctaaat aatgaagaat acttatatta ttggtgaaat
    13141 agggcagaat cataatggtt ctgttgactt ggctaagttg attattgaat taatctcacg
    13201 acctgtacat gaggatgttt ttggtatgga tttcatgcca atgaatgctg ttaaaatgac
    13261 taaaagagat ttatcagaag aattggctgt ttctcaaatg aatcaaatat atgacaatcc
    13321 taattctttt gggcgtactt atggtgaaca tcgtgctttt cttgagcttg atgatcaagc
    13381 gcattttgag atttataaat atgctaagtc attaggatta gattttatcg agacgttatg
    13441 tgccaaagga tgtctgtctt tgcttaaact gttcacaccg gataggctaa aagttgctag
    13501 tcgtgatttg actaacttac cattgcttga agcattagca gaaactcata ttcctattat
    13561 attatcgaca ggaatggctg gtcaaagaga acttgacaat gcattagatg tgataacgag
    13621 atatcatagt aatatagcta ttctacattg tgtatctcag tatccaactc atccaaataa
    13681 tttgaatcta cgtacaataa catatttgaa gaagcattac aataagtttg agataggttt
    13741 ttctgatcat acaataggta tttctgctgc tacagctgct gtagcgatgg gagcggaaat
    13801 tatagaaaag catgtaacaa ttgatcggca tatgaaagga acagaccagc ttggctcttt
    13861 aggtccagat ggggttaatc gtatgattcg agatattcgc atagcagagt gttggcttgg
    13921 tactgaagat ctttatatag aaaaaggggt ggagaagtct aagataaaac ttgaacgttc
    13981 tattgccact cgaaaatata ttccagcagg tagtattatt caagagtcgg acattcattt
    14041 actgagtcct ggtgatggtt ataaatggat agataaaaaa aatgtaattg gtcattttgc
    14101 taaacaagat atatcagcga atgaaataat atatcctgat tttattgaat agatatattc
    14161 ctactgcaac ttgtattacg gtatagagta ctgcctgcta taagctaagt agtgattaat
    14221 aaataagggt aaaatactaa aaaaatgaaa gagataaagc ttatacttac agatattgat
    14281 ggcgtttgga ctgatggcgg aatgttttat gatc
//
