LOCUS       gnlProkkaJOKNFAME_170  12818 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JOKNFAME_170.
ACCESSION   gnlProkkaJOKNFAME_170
VERSION     gnlProkkaJOKNFAME_170
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-01 14:23:09
            Original ID  :: gnl|Prokka|JOKNFAME_170
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12818
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..12818
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[3504:7818]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3505..7818
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..12818
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..12818
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             complement(3..185)
                     /locus_tag="ctg170_1"
                     /transl_table=11
                     /translation="MIFQHVGSILLSLFTKAGLVDIRTRGLGGVYIVLLLIELLHTHSL
                     FLGHTFTRKFGSKLFL"
     CDS             639..2282
                     /gene_functions="transport (smcogs)
                     SMCOG1186:polysaccharide export protein (Score: 71.1;
                     E-value: 1.1e-21)"
                     /gene_kind="transport"
                     /locus_tag="ctg170_2"
                     /transl_table=11
                     /translation="MSDIGSLRNVQLVRNGKNIATIDVYEFIMKGNTQDDIRLQEGDVV
                     IVPAYDVLVKISGKVKRPMRFEMKKDENLATLIKYAGGFEADAYTRSLRVVRQNGEEYE
                     VNTVKDIDYNIYKMRNGDVVTAEAILNRFTNKLEIRGAVYRPGIYQLSGKLNTIRELVH
                     EAQGLTGDAFLNRAVLYRQREDLTSEVVQIDIKSIMDGTSPNLALMKNDILYIPSIHDL
                     EDRGNVTVHGEVAHPDSYPYADNMTLEDLIIQAGGLKEAASTVRIDISRRIKNPRSTAD
                     NDTIGQMYTFSLKDGFVIDGQPGFILEPYDQVYVRRSPGYQAQQNVAIEGEILFGGNYA
                     MTSREERLSDLVNKAGGPTNYAYLRGAKLTRVANASEKKRMGDVIRLMSRQLGEAMIDS
                     LGIRVEDTFTVGIDLEKALSNPKSNADLVLREGDVISIPKNTNTVTINGAVMVPNTVSY
                     MKGKNVDYYLNQAGGCSDNARKSKKFIVYMNGQVTKVKGSGKKQIEPGCEIIVPSKAKK
                     KGNIANILGYATSFSSLGMMIASIANLIKK"
     CDS             2293..3441
                     /locus_tag="ctg170_3"
                     /transl_table=11
                     /translation="MIEKQSYQEPFSEVNKRKDDEMEIDLMAIFYKIITVRGVLCKAAG
                     VGVIIAIVVALSIPKQYTVKVTLSPEMGNAKGSNGLAGLAASFLGDGALIGESTDALNA
                     SLSADIVSSTPFLLELLEMEVSVSKKDDKMTLGSYLDEESSPWWDYLIGFPGIVIGGVK
                     SLFGEDTVPASGGRQGTIELTKEVNEKINFLKKKISASIDKKTAITSITVTLQNPQITA
                     VIADSVVHKLQEYIIGYRTSKVKEDCAYLERLFKERQQEYYAAQRKYADYVDAHDNVVL
                     QSVRAEQERLQNDMSLAYQIYSQVANQLQVSRAKVQEEKPVFAVVEPAVVPLKPSGMGL
                     KIYILLFVFLSVSGTLVWVFFGKSLLASLRKELKNKEIEQDK"
     CDS             3505..4224
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 54.5; E-value:
                     1.6e-16)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg170_4"
                     /sec_met_domain="NTP_transf_3 (E-value: 3.3e-16, bitscore:
                     50.8, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRTAMIMAAGMGTRFGQYTELIPKGFVKVGGIPMIVRSIDTLLS
                     CDIERIVIGTGYKQEVYEELKTDYPMLETCFSPRYAETNSMYTLYNTREILGNDDFLLL
                     ESDLIFEKQAIMSLLECPAADAMLITPVTKFQDQYYVEHDDNFRLSSCSVNKNKLNAKG
                     ELVGIHKLSGSFYKIMCADYASIVDEQPNLGYEYELLRISCSQSPVYVHKVEGLKWYEI
                     DDISDLEYAEKYIVPYC"
     CDS             4245..5546
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 208.9;
                     E-value: 1.5e-63)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg170_5"
                     /sec_met_domain="CTP_transf_like (E-value: 4e-13, bitscore:
                     40.6, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 4.1e-121, bitscore:
                     394.2, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 4.6e-63, bitscore:
                     203.6, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKTVYIGMTADIMHPGLIRIINEATKYGDVIIGLLTDKAIAEHK
                     RLPYLTYEQRKEVVQNIKGVCKVVPQEEWSYVENLKRIKPDYIIHGDDWKTGPLREERV
                     RVFEVMNEQGGKVIEIPYTLGINSSSLDKDIKAIGTTPDVRLKSLRRLINAKPVVRILE
                     AHDGLCGLIIENQEILKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAIIIEDKVGLKKNSLFGTDVIQTQDT
                     IEGFCNKIKAGKASQITDDFMIIARIESLIAGKPVSDALERAFAYVQAGADGIMIHSKN
                     KSGEDIKEFCLAFRKQYAHVPIVVVPTTYDHIYESELCDWGVNIIIYANHMLRAAYPAM
                     MNVAKIILENERALEVRELCMPIKEILELIPGTK"
     CDS             5563..6687
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 58.1;
                     E-value: 1.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg170_6"
                     /sec_met_domain="TPP_enzyme_N (E-value: 2.2e-20, bitscore:
                     63.7, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.9e-20, bitscore:
                     64.0, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSVEKVYETFLSHDVDFFTGVPDSLLKNICAYITDHTSREKHII
                     AANEGAAVGIASGYYMASGKVPVVYMQNSGLGNTVNPLLSLADEQVYSFPMLLMIGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMRIPYIILDSNENDAFIQLHDIIQSAKTKNIPHAII
                     IRKDVFGKYKLQKEFGCNYPLSREDALQIVVDYLPENSVVVSTTGKLSRELFEYREMKE
                     QGHEHDFLTVGSMGHSSSISLGIAIAKQDRPVYCLDGDGAFIMHLGAISNIGDLSPKNY
                     YHILFNNGAHESVGGQPTLGFSLDIPAIVRGSGYKHTYTVCTKVEIEEAMKQLPKLCGP
                     VLLEIKVKIDSRENLGRPTTTPIENKEHFMDFLK"
     CDS             6697..7818
                     /NRPS_PKS="Domain: Aminotran_5 (30-366). E-value: 2.9e-34.
                     Score: 110.4. Matches aSDomain:
                     nrpspksdomains_ctg170_7_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 76.3; E-value:
                     3.6e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg170_7"
                     /sec_met_domain="Aminotran_5 (E-value: 3.1e-31, bitscore:
                     99.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIERKILLNPGPATTTDSVKMAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGNLEKHTAVLFCGSGTINIDICLNSLLPEDKRVLIVNNGAYSTRAVEVCQYY
                     GLPHINLEFSVYERPDLSVVENVLQENPDIAMVYTTHHETGTGILNPIREMGAIAHKYQ
                     AKFVVDTTSSLGMIPFDIEKDNVDFCMASAQKGLQAMTGLSFIIGNEEQIKFSKKYPKR
                     SYYCNLYLQYENFERTGEMHFTPPVQTIYATRQALNEYFEVGEEAKFARHKRVFEAIHS
                     GINEIGLKSVIKREWQSGLVVSVQYPEDPNWDFEKIHDYCYERGFTIYPGKISTEDTFR
                     ICALGEIEVDDIENFFKVLKAALNHYNVTLPMS"
     aSDomain        6787..7794
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg170_7_Aminotran_5.1"
                     /evalue="2.90E-34"
                     /label="ctg170_7_Aminotran_5.1"
                     /locus_tag="ctg170_7"
                     /protein_end="366"
                     /protein_start="30"
                     /score="110.4"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGNLEKHTAVLFCGSGTINIDICL
                     NSLLPEDKRVLIVNNGAYSTRAVEVCQYYGLPHINLEFSVYERPDLSVVENVLQENPDI
                     AMVYTTHHETGTGILNPIREMGAIAHKYQAKFVVDTTSSLGMIPFDIEKDNVDFCMASA
                     QKGLQAMTGLSFIIGNEEQIKFSKKYPKRSYYCNLYLQYENFERTGEMHFTPPVQTIYA
                     TRQALNEYFEVGEEAKFARHKRVFEAIHSGINEIGLKSVIKREWQSGLVVSVQYPEDPN
                     WDFEKIHDYCYERGFTIYPGKISTEDTFRICALGEIEVDDIENFFKVLKAALNHY"
     CDS             7815..7958
                     /locus_tag="ctg170_8"
                     /transl_table=11
                     /translation="MIAEILLKIRHFYFSLRGIHFHPESVISGVPIRSFRLVGKYGNIN
                     LR"
     CDS             8197..9645
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg170_9"
                     /sec_met_domain="Polysacc_synt (E-value: 3.1e-31, bitscore:
                     99.6, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWSAIERFSLQGVQFIIQIILARLLLPSDYGMIGMLAIFLQVAQV
                     FIDSGFTNALIQKKDRTEEDFATVFYFNILVAVLFYGILFFSASLIADFYNMPTLVLVI
                     RFIALSLIINALSAIHRTKLIISVNFKTQSKISLGAAFISGVIGIWMAYVGCGVWALVW
                     QTLLNSIILTILFYCLVHWKPLHTFSKSSFERLFNFGSKLLISSLINTVYRNLYTIVIG
                     KKFSVVELGYYTRAEQFAIFPSNNLNALVSRVAYPILSSIQDDDKCLTNAYRNYIRLSS
                     YIIFPLMVGLLVLANPIISLLLTDKWSGVVILLQILCLDWMFDHLSQINLNLLWVKGRS
                     DLSLRLEIIKKTIAIFILFVSIPFGLEVMCWGRVIYSLIATYLNTYYTNSLIDLTLKLQ
                     VKDVFPSLILSFIMGGIVFICISFFETDIVKIVIGCITGMSFYILLSFLFKLDSFFCIL
                     TLIIKTKNIDENIKKDFSSNSKDN"
     CDS             9599..10858
                     /locus_tag="ctg170_10"
                     /transl_table=11
                     /translation="MKILKKIFQVIVKIINHCVPVDSRSLYFIPHPNCRTDKYDIINYS
                     SDNVLVLLNYLLKKDSAEYYKIYVEIYDSSRISEYQEYIYGINPNIRCTFLCACVGRFF
                     FNKVSFKDMLYAFFCFCRASKCFTATFYYDFSFKKRSQQIICLGYYTPFKDDYHMGDHS
                     YDKFRNTTCNSFDYSIATSCLSARIISIDCGISYDKFKVLGFPRNDLLISKNNCNVIKR
                     EISKFAGYDVTKYIVYTPTFRDYETVTEGNLRSILGYVDCDLLKLSKILLKFNAALILK
                     LHPLQNKTVLKKDLPKGILVFEQTYKYGLYDLLSFSDGMITDYTSTYFDFLLVNKPVIF
                     NFYDIEEYRRVRGFSFEPIEFFCAGDIVYNYNELIDAVMGLLAGKDIHAEHRRHISLLM
                     NQFQDDNSTKRICDIFLNEI"
     CDS             10867..11616
                     /locus_tag="ctg170_11"
                     /transl_table=11
                     /translation="MNYAFVVQARLGSTRLPGKILKPFYGNQSILDLMVHKLSAISNIP
                     VIIATTNSVINEPIEKKALALGVKCFRGEENDVLKRFIDVAEYFDIQGIFRICSDNPFL
                     DVHAARQLVEIAMKSCNDYISFDIDGTPSIKTHFGFWGEFVTLDALKRVIGFTDDLLYR
                     EHVTNYIYSHPELFNIQWISGSPVVSKHHNIRLTIDTLEDFSVAQRIYRDLQEKRVEIS
                     IEVIVDYVRRHAEYIQLMKQQIKKNSK"
     CDS             11616..12656
                     /locus_tag="ctg170_12"
                     /transl_table=11
                     /translation="MKNTYIIGEIGQNHNGSVDLAKLIIELISRPVHEDVFGMDFMPMN
                     AVKMTKRDLSEELAVSQMNQIYDNPNSFGRTYGEHRAFLELDDQAHFEIYKYAKSLGLD
                     FIETLCAKGCLSLLKLFTPDRLKVASRDLTNLPLLEALAETHIPIILSTGMAGQRELDN
                     ALDVITRYHSNIAILHCVSQYPTHPNNLNLRTITYLKKHYNKFEIGFSDHTIGISAATA
                     AVAMGAEIIEKHVTIDRHMKGTDQLGSLGPDGVNRMIRDIRIAECWLGTEDLYIEKGVE
                     KSKIKLERSIATRKYIPAGSIIQESDIHLLSPGDGYKWIDKKNVIGHFAKQDISANEII
                     YPDFIE"
ORIGIN
        1 aacagaaaca gcttgcttcc gaacttgcgc gtaaaggtgt gaccaaggaa caggctatgc
       61 gtgtgaagca gctctatgag cagcagaaca atgtaaacac ctcccaatcc acgggtacgg
      121 atatcaacga gtcccgcctt cgtgaagaga ctaaggagaa tacttccgac atgctggaag
      181 atcatccgac tacggaagat ttggtccgtg aggaccaggt tttcgggcgc aacatcttca
      241 atacccgcaa cctgacgttc gagcctagtg tcaaccttgc cactcctgcc aattaccgtc
      301 tgggtcccgg tgatgaagtc atcatcgata tctggggtgc tagccagaat acgatccgcc
      361 agcagatctc tcctgaaggc accatcaata ttcagaagat cgggcctgtc aaccttagcg
      421 gtatgacagt ctccgccgcc aacgactatc tgaagaatgc cctgaataag atctataacg
      481 gcctgaacaa tactaccgac cctacttccg acatccgtct gacgttaggt aatatccgta
      541 ctatccagat caatgtgatg ggagaggtcg tgcagccggg tacctacgcc ctttcttcct
      601 tctctaccgt cttccacgcg ctttaccgtg caggcggtgt gagtgatatc ggcagcctcc
      661 gaaacgttca gttggtacgc aacggaaaga atatcgccac catcgatgtt tacgaattta
      721 tcatgaaagg caacacgcag gatgacatcc gccttcagga aggtgacgtg gttatcgtcc
      781 ccgcctacga tgtcttggta aagatcagcg gcaaggtaaa acgccctatg cgtttcgaaa
      841 tgaagaaaga cgagaacctt gccactttaa tcaaatatgc cggcggcttt gaagctgatg
      901 cctacacccg ttcattacgt gttgtccgtc agaatggtga ggaatacgaa gtcaacacag
      961 tcaaagatat agattacaac atttataaaa tgcgtaacgg tgacgtagta accgccgaag
     1021 ccatccttaa ccgcttcaca aacaaactgg aaatccgtgg cgctgtctat cgtcccggta
     1081 tctaccagct cagcggtaaa ctgaacacga tccgcgaact tgtccatgaa gctcagggtc
     1141 tgaccggtga cgctttcctg aatcgtgccg tcctctatcg ccagcgtgaa gacctgacca
     1201 gtgaagttgt tcagatagac attaagtcta tcatggacgg cacttcccca aaccttgccc
     1261 taatgaagaa tgacatcctc tacattccaa gtatccatga cctggaagac cggggtaatg
     1321 tcacagttca cggtgaggtt gcccaccctg attcctatcc ttatgcggat aacatgacac
     1381 ttgaggatct gatcattcag gccggtggct tgaaagaagc cgcctctact gtccgtattg
     1441 atatatcgag acgaatcaag aatcctcgta gtactgcgga taatgatacg attggtcaga
     1501 tgtatacctt ctctttaaag gatggttttg taatagatgg tcagcccggt tttatccttg
     1561 agccttatga tcaggtttat gtccgtcgca gtccgggcta tcaggcacaa cagaatgtag
     1621 ccattgaagg tgaaatcctt ttcggaggaa attacgccat gaccagccgt gaggaacgtc
     1681 tttccgattt agtgaataaa gccggcggtc cgaccaatta tgcctatctg cgtggtgcga
     1741 aactgacccg tgtggctaac gctagtgaaa agaaacgtat gggtgatgta attcgcctta
     1801 tgagcaggca attgggcgaa gccatgattg actctttggg tattcgtgtt gaagatactt
     1861 ttacggttgg aatagatttg gaaaaagcct tgagcaatcc taaaagtaat gccgatcttg
     1921 tcttacgtga aggtgatgtg atctccatac ctaagaatac taatactgta actatcaatg
     1981 gtgctgtcat ggttccgaat acggtttctt atatgaaggg aaagaatgtg gattattacc
     2041 tgaatcaggc cggtggctgt tctgataatg caaggaagag taagaagttc attgtttaca
     2101 tgaatggtca ggtgacgaag gttaagggta gtggtaagaa acagattgag ccgggatgtg
     2161 agattattgt gcctagtaag gctaagaaga aagggaacat agctaatatt ttaggttatg
     2221 ctacttcctt cagttctttg ggaatgatga ttgcttctat tgctaatttg ataaagaaat
     2281 aaatgaccta ttatgattga aaagcaatct tatcaagaac cgttttctga agtgaataaa
     2341 aggaaagatg atgaaatgga aatagacttg atggctattt tttataagat tattactgtt
     2401 cgtggagtac tatgcaaagc tgctggagtg ggagtaataa ttgctatagt ggtggcattg
     2461 agcattccca agcaatatac agtaaaggtt accctttctc ctgaaatggg taatgcgaag
     2521 ggaagtaatg gtttagccgg acttgctgcc tcgtttttgg gagatggggc tctaataggg
     2581 gaaagcacag atgccttgaa cgcttctctt tcagcagata ttgtttcttc aactcctttt
     2641 ttattggaac ttttagaaat ggaagtctcc gtttctaaaa aggatgataa aatgactttg
     2701 gggtcttatc tcgatgaaga gtcatctcct tggtgggatt atcttatagg ttttccgggc
     2761 atagtaatag gaggagttaa atcattattc ggagaagata cggttcctgc cagtggaggg
     2821 cggcaaggaa ctatcgaatt gacgaaagaa gtaaatgaga agattaattt tcttaaaaag
     2881 aagattagtg catccattga taagaaaacc gccataacga gcattacggt caccttacaa
     2941 aatccgcaga tcactgcggt tatagcagat tctgtcgttc ataaattaca ggaatatatt
     3001 attgggtatc gaacttcaaa agtaaaagaa gactgtgcgt atttggaacg gttgtttaaa
     3061 gaacgtcagc aggaatatta tgctgctcaa agaaaatatg cggattatgt agacgcccat
     3121 gataatgtgg ttcttcaaag tgtacgtgct gagcaggagc gtttacagaa cgacatgagt
     3181 ttggcttatc agatatatag tcaggttgct aatcagttgc aggtatccag agccaaagta
     3241 caggaggaaa agccagtctt tgcagtagtt gagcctgccg ttgtaccttt gaaaccttca
     3301 ggtatgggac tcaaaattta tattttatta tttgtgttcc tgtccgtatc tggtactctc
     3361 gtgtgggtgt tttttggcaa aagtctgctg gctagcttga gaaaagaact gaaaaataag
     3421 gagatagaac aggataaatg atgctaaaac gtcgtttaca acattcttac atcaattaaa
     3481 ttcacttact atattattaa acgaatgata cgtacagcta tgataatggc tgcgggcatg
     3541 ggaacccgtt ttggccaata tacagaattg atacctaaag ggttcgtcaa agtgggaggt
     3601 attccaatga tagttcgttc tattgatact ttgttatcat gtgacattga gcgaattgta
     3661 atcggtacag gatataaaca agaagtttat gaagaattga agacagacta tcctatgctt
     3721 gaaacctgtt ttagtcctcg ttatgctgaa acaaatagta tgtatacttt atataatacg
     3781 cgggagattc tgggcaatga tgatttttta ttgttagagt cagatttgat ttttgagaaa
     3841 caagcaataa tgagcttatt ggagtgtcct gcggcagatg caatgctaat aactccagtg
     3901 acaaagtttc aagatcagta ttatgtggaa catgatgata attttcgcct ttcttcatgt
     3961 tctgtcaata agaataaact aaacgctaaa ggcgaattgg tcggcatcca taaactatcc
     4021 ggttcctttt acaaaataat gtgtgctgat tatgcgagta ttgttgatga acaacccaat
     4081 ttgggatatg agtatgaatt actacgaata tcttgttctc aatctcctgt ttatgtgcat
     4141 aaagtagaag ggctgaaatg gtatgaaatc gatgacatat cagatttgga atatgcagag
     4201 aaatatattg ttccgtattg ttaattaaaa taatatatca aattatgagt aagacagttt
     4261 atattgggat gactgcggat attatgcatc caggacttat ccgcattatt aatgaggcta
     4321 ctaaatacgg tgatgttata attggtcttc ttacagataa agctattgcg gagcataaac
     4381 gccttcctta tctgacctat gaacaacgaa aagaggtagt tcagaatatt aaaggcgtat
     4441 gtaaagttgt tcctcaagaa gaatggagct atgttgagaa tctcaaacgc attaaacctg
     4501 actatataat tcatggtgac gactggaaaa ccgggccttt acgtgaagaa cgtgtgcgcg
     4561 tatttgaagt gatgaatgaa cagggaggaa aggttatcga aattccatat actttgggaa
     4621 ttaattcatc ttctttggat aaagatatta aggctatagg tacaacccct gatgtacgtt
     4681 taaaatcact tcgtcgtttg ataaatgcca aacccgtagt tcgtattttg gaagctcatg
     4741 atggactatg tggcttgata attgaaaatc aggaaatatt aaaaggggat aagagggaag
     4801 tatttgacgg aatgtggtct agtagcctta cggattcaac cagtaaaggt aaacctgata
     4861 ttgaggcggt tgatttaact acccgattgc aagatttgaa taatattctg gagtgtacta
     4921 ctaaacctat tatttttgat ggagatacag gtggtaaaat tgaacatttc gtatttacag
     4981 tacgcacttt agaacgtcat ggaatttcag ctataataat tgaagataaa gttggattaa
     5041 aaaagaattc attgtttggt acagatgtta ttcaaactca agatacaatc gaaggtttct
     5101 gtaataagat caaagcagga aaggcatctc agattactga tgattttatg attatcgctc
     5161 gtattgagag ccttatagcg ggaaagccgg taagtgatgc tttagagaga gcttttgcat
     5221 atgtccaagc gggagcggac ggtattatga tacatagcaa aaataagtcg ggtgaagata
     5281 taaaagaatt ttgtttggcg tttcgaaaac agtatgctca tgtacctatc gtggttgtgc
     5341 ctactacata tgatcatatt tatgagtctg aattgtgtga ttggggtgtg aatataatta
     5401 tttatgctaa tcatatgctt cgtgccgctt atcctgctat gatgaatgtc gcaaagataa
     5461 tattagagaa tgaacgtgct ttagaggtgc gcgaactgtg tatgcccata aaagaaatat
     5521 tggaactgat tccaggtact aaataaaaag aggaatatca ttatgttaag cgtagaaaaa
     5581 gtttatgaaa cgtttctgag tcatgacgtt gattttttta caggagttcc agactcatta
     5641 ctaaaaaata tttgtgccta tatcacagac catacttcac gtgagaaaca tatcatagct
     5701 gctaatgaag gtgcggctgt aggaattgct tcaggttatt atatggcatc aggtaaggtt
     5761 cctgtggttt atatgcaaaa ttccggcttg ggtaatactg ttaatcctct gctgtctctt
     5821 gcagatgaac aagtatacag ttttccaatg cttttgatga ttggctggcg tggtgaaccg
     5881 ggaacaaaag atgaacctca gcataaaaaa cagggtgagg taactttaga tttgttgaaa
     5941 gcaatgcgga ttccttatat aattcttgat tcaaatgaga atgatgcatt tattcagctc
     6001 catgatatca tacaatcagc aaaaactaag aatattcctc atgcaataat tattcggaaa
     6061 gatgtttttg gcaaatataa acttcaaaaa gagttcggat gtaattatcc tttatctcgt
     6121 gaggatgctt tgcaaatagt tgtggattat ctgcctgaaa attcagtggt cgtttctact
     6181 actggcaagc tttctcgtga gttatttgaa tatcgagaaa tgaaagagca aggtcatgaa
     6241 catgattttc tcacggtagg ctctatggga catagttcat caatctcttt gggtattgct
     6301 atagcaaagc aagatagacc tgtttattgc ttggatggtg atggagcatt tattatgcat
     6361 ttaggggcaa ttagtaacat tggcgattta tctcctaaaa actattatca tattttgttt
     6421 aacaatggtg ctcacgagtc tgtaggagga cagcctactt taggatttag tttagatatt
     6481 ccggctattg tccgggggag cggatataag catacttata ctgtttgtac aaaagttgaa
     6541 attgaagaag caatgaagca gcttccaaag ttatgtggtc ctgttttgtt ggaaataaag
     6601 gttaaaatag attctcggga gaatttggga cgtcccacga ctactcctat agagaataag
     6661 gagcatttta tggatttttt aaaataaata tattcgatga aaatagaaag aaaaatatta
     6721 ttaaatcctg ggcctgccac tactactgac agtgtaaaaa tggcacaagt tgttccggat
     6781 atttgtcctc gcgagaaaga attcgcaggt atgatgaaac aacttcggga cgatttggtc
     6841 agagttgctc atgggaattt ggaaaaacat accgctgtat tattttgtgg ctcaggaacc
     6901 atcaatattg atatatgtct aaattctctt cttcctgaag acaaaagggt gcttatagtg
     6961 aataatgggg catatagtac ccgtgctgta gaagtgtgtc aatattatgg tttgcctcat
     7021 attaatttgg aattttccgt ttacgaacgt cctgatttat cggtggtgga aaatgtgttg
     7081 caagaaaatc ctgatatagc tatggtttat actactcatc acgagacggg tacaggaatt
     7141 ctgaacccta ttcgcgagat gggcgctata gctcataaat atcaggctaa atttgtcgtc
     7201 gatactactt cttctttggg catgattcct tttgatatag agaaagataa tgtggatttt
     7261 tgtatggcat ctgctcaaaa aggacttcaa gcgatgacag gtctttcttt tattataggc
     7321 aatgaggaac agattaagtt ttcaaaaaaa tatcccaagc gttcttacta ttgtaatctt
     7381 tatttgcagt atgagaattt tgagagaaca ggtgaaatgc attttactcc accggtacaa
     7441 actatttacg ccactcgtca ggcattaaat gaatattttg aagtgggtga agaagctaag
     7501 tttgcccgtc acaaaagagt ttttgaggct attcattcag gtataaatga aattggtctt
     7561 aaaagtgtaa taaagcgtga atggcagtca ggactggttg tctcagttca atacccagaa
     7621 gatcccaact gggattttga gaaaatacat gattattgtt atgagcgtgg atttacgatt
     7681 tatccaggta agatatcaac tgaggatact tttagaatct gtgctttagg agagatagaa
     7741 gtagatgata ttgagaactt ctttaaagta ctaaaagctg cattaaatca ttataacgtc
     7801 acattaccga tgtcatgatt gcagaaatat tattgaagat tcgtcatttt tatttttccc
     7861 ttcgtggtat acacttccat ccggaaagtg ttatttctgg agtgcctatt cgtagctttc
     7921 gcttagtagg caaatatggc aatattaatt tgaggtgatg cattaggagt tctttattct
     7981 tataaaaagg catcggtagt tataggagat aatgtttgtg tagggtaatt tgcttttagt
     8041 gggagagacc atattagaat tgcggtaagg ggcgaagaag ataataacca attagtgaga
     8101 atattggaaa aatataattg atatcggtaa atgtagttgt ttttatactg tatttaagta
     8161 atggaaaatc taaagcagaa aactgtatca ggaataatgt ggagcgctat agaacgtttc
     8221 tctttacaag gagtacagtt tataattcaa ataatattag ctcgtttgtt actaccatcg
     8281 gattatggta tgatcgggat gttagctatt tttcttcaag tcgctcaagt atttatagat
     8341 agcggtttta caaatgcttt aatccagaaa aaagatagaa ctgaagagga ttttgccact
     8401 gtcttttatt ttaatattct tgtcgcagta ttattctatg gtatattgtt tttttcggct
     8461 tctttgattg cagactttta taatatgccg actttagttc ttgtaattcg ttttatagca
     8521 ttgtcgttga taataaatgc tttatcggct attcacagaa cgaaattaat tatttctgta
     8581 aactttaaaa ctcaatctaa aatttcatta ggtgcagctt ttatatcagg cgtgattgga
     8641 atatggatgg cttatgtcgg ttgcggggtt tgggctttag tttggcaaac tctcttaaat
     8701 agtataattt tgacaatttt gttttactgt ttagttcatt ggaaaccttt acatactttc
     8761 tccaaaagtt cttttgaaag attattcaat ttcggatcta aattattgat ctcaagtctt
     8821 atcaatacag tatatcgtaa tctgtatacc attgtaatag gaaaaaagtt ttctgttgta
     8881 gagcttggtt actatacaag agcagaacaa tttgctattt ttccttccaa taatttaaat
     8941 gctttagttt ctagagtagc gtatcctatt ttaagttcca ttcaagatga tgataaatgt
     9001 ttaacaaacg catatcgaaa ttatatcaga ttatcctcat atataatttt tcctttaatg
     9061 gtaggtttac ttgttttggc taatcctatt attagtctat tgttaacaga taaatggtct
     9121 ggggttgtta ttcttttaca gatattatgt ttggattgga tgtttgatca tttgagccaa
     9181 attaatttga atttattatg ggttaaagga agatctgatc tgtcgttacg tcttgaaata
     9241 ataaaaaaga cgattgctat attcattctt tttgtgtcaa ttccatttgg gcttgaggtg
     9301 atgtgttggg gtagagttat ttactctctg atagcgacat atttgaatac atattatact
     9361 aattctctta ttgatttgac cttaaagttg caagttaaag atgtatttcc aagtttaata
     9421 ctctctttca taatgggggg gatcgttttt atatgtattt ctttctttga aactgatata
     9481 gtaaaaattg tgattggatg tattactggg atgtccttct atattttact ttcttttctt
     9541 tttaagctag attctttttt ttgtatttta acattgatca ttaaaactaa aaacatagat
     9601 gaaaatatta aaaaagattt ttcaagtaat agtaaagata attaaccatt gtgtgccagt
     9661 tgatagtaga tcattgtatt ttattcctca tcctaattgt agaacagata aatatgatat
     9721 tataaattat tcgtcagata atgttctcgt tctgttaaat tatttgctaa aaaaagatag
     9781 tgctgaatat tataaaatat atgttgaaat ttatgattct tctcgtattt cagaatatca
     9841 agaatatatt tatggcataa atcctaatat taggtgtact tttttatgtg catgtgttgg
     9901 acgttttttc tttaataaag tatcttttaa ggatatgttg tatgcctttt tttgtttttg
     9961 tagagcaagt aaatgtttta cagctacttt ttattatgat ttttcattta aaaaacgctc
    10021 tcaacagatt atttgtttgg ggtattatac tccattcaaa gatgattatc atatgggaga
    10081 tcattcttat gataaatttc gtaatacaac atgtaactca tttgattact ctattgctac
    10141 ttcttgttta agtgctagaa taatttcaat agattgtgga atctcatatg ataaattcaa
    10201 agtcttagga tttcccagaa atgatttgtt gatatcgaaa aataattgca atgttattaa
    10261 aagagaaatt tcgaaatttg cgggatatga tgtaactaaa tatatagttt atactccaac
    10321 ttttcgtgat tatgagactg ttactgaagg taatttgcgt tcaatactcg gatatgttga
    10381 ttgtgatttg ttgaagttat cgaaaatact attgaagttt aatgcggctt taattcttaa
    10441 attacatcct ttgcagaata aaacagtctt aaaaaaagat ttacccaaag gtatacttgt
    10501 ttttgaacaa acatataaat atggattgta tgatcttttg tcattttcag atggaatgat
    10561 tactgattat acttctacct attttgattt tttattggta aataagccag tcatatttaa
    10621 tttctacgat attgaagaat atcgtagagt aagaggtttc tcatttgagc caatagaatt
    10681 tttttgtgca ggtgatattg tttacaatta taatgaattg atagatgctg tgatgggact
    10741 tctggctggg aaagatattc atgctgaaca tcgacgtcat atttctttat tgatgaatca
    10801 atttcaagat gataattcaa caaagagaat atgtgacata tttttaaatg aaatataata
    10861 aataaaatga attatgcatt tgtggtgcag gcacgtttag gttctacacg tttgcctgga
    10921 aaaatattga aacctttcta tggtaatcaa tcgattcttg acttaatggt tcataagctt
    10981 tctgctatat cgaatattcc agtaattatt gctacaacaa attctgttat caatgaacct
    11041 attgaaaaga aggcacttgc attaggggta aagtgcttca gaggtgagga gaatgatgtt
    11101 ttaaaacgat tcattgatgt tgctgaatat tttgatatac agggtatatt ccgaatatgt
    11161 tctgataatc catttttaga tgtacatgcg gcaaggcaat tggtcgaaat agcaatgaaa
    11221 tcttgtaacg attatataag ttttgatatt gatggtactc catcaattaa aactcatttc
    11281 ggtttttggg gagaatttgt aacgcttgat gcgctgaagc gagttatagg atttactgat
    11341 gatttattgt atcgtgaaca tgttactaat tatatatata gtcatcctga actttttaat
    11401 attcaatgga tttcaggatc tccagtcgtg tcaaaacatc ataatattcg actcacgatt
    11461 gatacgttag aagatttttc tgtagcacag agaatatata gggatttaca agaaaaaaga
    11521 gtggaaatat cgatagaagt gattgtagac tatgttcgaa gacatgcaga atacattcaa
    11581 ctgatgaaac aacaaataaa aaaaaattct aaataatgaa gaatacttat attattggtg
    11641 aaatagggca gaatcataat ggttctgttg acttggctaa gttgattatt gaattaatct
    11701 cacgacctgt acatgaggat gtttttggta tggatttcat gccaatgaat gctgttaaaa
    11761 tgactaaaag agatttatca gaagaattgg ctgtttctca aatgaatcaa atatatgaca
    11821 atcctaattc ttttgggcgt acttatggtg aacatcgtgc ttttcttgag cttgatgatc
    11881 aagcgcattt tgagatttat aaatatgcta agtcattagg attagatttt atcgagacgt
    11941 tatgtgccaa aggatgtctg tctttgctta aactgttcac accggatagg ctaaaagttg
    12001 ctagtcgtga tttgactaac ttaccattgc ttgaagcatt agcagaaact catattccta
    12061 ttatattatc gacaggaatg gctggtcaaa gagaacttga caatgcatta gatgtgataa
    12121 cgagatatca tagtaatata gctattctac attgtgtatc tcagtatcca actcatccaa
    12181 ataatttgaa tctacgtaca ataacatatt tgaagaagca ttacaataag tttgagatag
    12241 gtttttctga tcatacaata ggtatttctg ctgctacagc tgctgtagcg atgggagcgg
    12301 aaattataga aaagcatgta acaattgatc ggcatatgaa aggaacagac cagcttggct
    12361 ctttaggtcc agatggggtt aatcgtatga ttcgagatat tcgcatagca gagtgttggc
    12421 ttggtactga agatctttat atagaaaaag gggtggagaa gtctaagata aaacttgaac
    12481 gttctattgc cactcgaaaa tatattccag caggtagtat tattcaagag tcggacattc
    12541 atttactgag tcctggtgat ggttataaat ggatagataa aaaaaatgta attggtcatt
    12601 ttgctaaaca agatatatca gcgaatgaaa taatatatcc tgattttatt gaatagatat
    12661 attcctactg caacttgtat tacggtatag agtactgcct gctataagct aagtagtgat
    12721 taataaataa gggtaaaata ctaaaaaaat gaaagagata aagcttatac ttacagatat
    12781 tgatggcgtt tggactgatg gcggaatgtt ttatgatc
//
