LOCUS       c00493_gnlPro..         2922 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AFKDHDOF_493.
ACCESSION   c00493_gnlPro..
VERSION     c00493_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 23:54:30
            Original ID  :: gnl|Prokka|AFKDHDOF_493
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2922
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..138
                     /locus_tag="ctg493_1"
                     /transl_table=11
                     /translation="VIMYLIPFFGKRKRDRVVREVRYHTTCKEVCKVKTTEREKEHEKD
                     "
     protocluster    1..2922
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[566:2922]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      567..2922
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2922
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2922
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             125..574
                     /locus_tag="ctg493_2"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVVGEFSIN"
     CDS             complement(567..1295)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 71.7;
                     E-value: 2.5e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg493_3"
                     /sec_met_domain="Radical_SAM (E-value: 8.9e-30, bitscore:
                     92.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKTLVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(1309..2922)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg493_4"
                     /sec_met_domain="Gly_radical (E-value: 1.5e-47, bitscore:
                     148.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 2.1e-247, bitscore:
                     810.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="RLREEVAEQIKALKDMKVMGEYYGLDLSRPAYTAQEAVQWVYMAY
                     LAAVKEQDGAAMSLGNVSSFLDIYLEYELSKGTITESFAQELIDQFVIKLRMVRHLRMQ
                     SYNDIFAGDPTWVTESLGGRLNDGRTKVTKTSFRFLQTLYNLGPSPEPNLTVLWSPELP
                     EGFKEFCAKVSIDTSSIQYENDDLMREVRQSDDYGIACCVSYQEIGKQIQFFGARCNLA
                     KALLLAINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNYKKVLTEIARVYNEAMNIIH
                     YMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTARRNDIGLTEGF
                     DIEGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVMYGKKT
                     GATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISNTFSIVPKSLG
                     ATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPENYPQLTIRVSGYAVNF
                     VKLSREHQLEVISRSFHERM"
ORIGIN
        1 gtgatcatgt atctgattcc tttcttcgga aagagaaagc gggacagggt ggtgagagaa
       61 gtgcgctatc atactacttg taaggaggtg tgcaaagtga aaacgacgga aagggaaaag
      121 gaacatgaga aagattagtc tgattgtgat ccattgttcc gccacccgtg tcgattgcga
      181 ctttacggca aaggatgtgg atacggctca ccgttatcgt ggcttttcgt gctgggggta
      241 tcattattat atacgcaagt cgggcgagat tgagccgatg cgggatgaag atacggtcgg
      301 agctcatgca cgtggctata atgcgatcag cttaggtgta tgttatgagg gcggtctgga
      361 tgagaatgga aaagctgcgg atacgcgtac accccgtcag aaggaggcgc tacatcgctt
      421 ggtacacgaa ttgttgcagc gttatccgga agctaaagta gtagggcatc gggatttaag
      481 tcccgacacc aactataatg ggatcgtaga tccttgggaa cggattaaag aatgcccttg
      541 ctttgaggtc gttggagaat tctccattaa ttaactacca ccgtagtgaa atactctttg
      601 aatacctccg cagctttttc aagctgttcg ggggtatttt cttttacccc tttcatcttg
      661 tattcctgtc ccatcgcttc gtacttatgt acgcccagtg tatggtaagg cagtatttcc
      721 acccgttgga tcatcttgta ttttccgagt gcttctccca gtgcgcgtat gtcttcttca
      781 aaatcgctgt atccgggcac tagcacgtag cgtagccaga aaggtttctc ctgttcttcc
      841 agccaggcgg ccgtgcggat ggtttgctcg ttgcttcttc cggtgagcgt ctgatggcgg
      901 ttggggttga attcctttat atccaataac acaagatcgg tcagtctgaa taattcttct
      961 acgtcttcgt tccaaagtcc tccgttgctg tccagacaga catggatgcc tttttctttc
     1021 agttcgcgga ccagcgggac gagtgttttc gcctgaaacg tcggttcgcc tccggagaaa
     1081 gtgattcctc ctcgtttccc gaagaagggg cgctggctca ttgccatgcg gacgatttct
     1141 tccggtgggg taggcgtgcc tccctttcct gctatcgtat ccggattggc gcaatagagg
     1201 cagcggaagt tgcatccttg aagaaaaacg acgagccgta agcccggccc gtcgaatgtt
     1261 cccatacttt cgtatgaatg tacgtttatc atcatataga gtaatagatt acatacgttc
     1321 gtggaagcta cggctgataa cttccagttg gtgttcgcgg ctcagcttca cgaagtttac
     1381 ggcatagccg gaaacgcgga tagtcagctg cggatagttt tccggatgct ccatggcatc
     1441 gtacagcata tcacggttca gtacgtttac gttcaggtgg tgagcacctt tggtgaagta
     1501 accgtccatc atcgttacga ggttctcgat acggtcttcg tccgtagcac ccagtgattt
     1561 cggaacgata gagaaggtgt tgctgatacc gtcctgtgag tcacggtaac gaagtttcgc
     1621 tacagagctc agagaagcga tcgcaccatt cttgtcgcgt ccgtgcatcg ggttggcacc
     1681 cggagcgaag gcaacacctt tggcacgtcc gtcgggagta gcaccggtct ttttgccgta
     1741 catcacattg gaagtgatag tcagcaagga gagggtagga cgggcattct tgtaaactgg
     1801 caatttcttc aattcttcgc tgaagaagta taccaggtct acgccaaggt ggtctacctt
     1861 gtcgttgtcg ttaccgaagc aagggaattc tccttcgatg tcgaaacctt ctgtcaggcc
     1921 gatatcgttg cggcgggcag ttactttcgc atatttgatg gcagacagtg aatcgagggc
     1981 gatggaaagt ccggctacac cgtaagccag gttgatgcgc gggttcgtat ctacaagagc
     2041 catctgggcc ttctcgtagt aatacttgtc gtgcatatag tggatgatgt tcatcgcttc
     2101 gttatatacg cgggcgattt cggtcagcac ttttttgtag ttgtccatta cttcttcgaa
     2161 cttcagcgtg tcactggtca gtacggggat gttcttcacc atgactgtac cagtattctc
     2221 gcaacgtccg ccgttgatgg caagcaacag ggctttggcc aggttgcaac gggcaccgaa
     2281 gaactgaatc tgcttgccta tttcctggta tgatacgcag caggcgattc cgtagtcgtc
     2341 ggactgacgt acttcacgca tcaggtcgtc gttttcgtac tggatagaag aagtgtcgat
     2401 agaaaccttt gcacagaatt ctttgaatcc ttccggaagt tccgggctcc agagtacggt
     2461 caggttcggt tccggtgaag ggccgaggtt gtacaatgtc tgcagaaaac ggaaggaggt
     2521 ctttgtcact ttggtacgtc cgtcgttgag acgtccgccc agagattcgg ttacccaggt
     2581 cgggtcgcct gcaaagatgt cgttgtatga ttgcatacgc aggtggcgga ccatacgcag
     2641 tttgatgaca aactggtcga tcagctcttg cgcgaacgat tcggtgatgg ttcctttgct
     2701 cagttcatat tccagataga tatcgaggaa agaagaaacg ttacccagtg acatagcggc
     2761 accgtcttgt tctttgacgg cggcaaggta agccatatat acccactgta cggcttcttg
     2821 tgccgtgtaa gcgggacggc tcaggtcgag accgtagtat tcgcccatca ctttcatgtc
     2881 tttcagtgcc ttgatctgtt ctgccacttc ttcgcgcagg cg
//
