LOCUS       c00173_gnlPro..         4373 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FNMJEHCP_173.
ACCESSION   c00173_gnlPro..
VERSION     c00173_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 20:27:42
            Original ID  :: gnl|Prokka|FNMJEHCP_173
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4373
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4373
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1827:4372]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1828..4372
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4373
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4373
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..158)
                     /locus_tag="ctg173_1"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GEKAANE"
     CDS             complement(245..475)
                     /locus_tag="ctg173_2"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             669..1172
                     /locus_tag="ctg173_3"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLIFRPGKTLAELMKILSYQQVQTRAVAQAGAGDGGGEDPDKNPDSGGDGSGDEEAP
                     DPTV"
     CDS             1232..1399
                     /locus_tag="ctg173_4"
                     /transl_table=11
                     /translation="MKEIVSRILDVIMYLIPFFGKRKRDRVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             1386..1835
                     /locus_tag="ctg173_5"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(1828..2556)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.7;
                     E-value: 3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg173_6"
                     /sec_met_domain="Radical_SAM (E-value: 5e-30, bitscore:
                     94.0, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCPYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(2570..4372)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg173_7"
                     /sec_met_domain="Gly_radical (E-value: 3.2e-47, bitscore:
                     148.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 3.7e-288, bitscore:
                     945.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DVYTEEIRSFRSLGFLTGLPDNYARGRIIGDYRRMALYGIDRLIE
                     AKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMKVMGEYYGLDLSRPAYTAQEAVQWV
                     YMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSKGTITESFAQELIDQFVIKLRMVRH
                     LRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKTSFRFLQTLYNLGPSPEPNLTVLWS
                     PELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQSDDYGIACCVSYQEIGKQIQFFGAR
                     CNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNYKKVLTEIARVYNEAM
                     NIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTARRNDIGL
                     TEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVMY
                     GKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISNTFSIVP
                     KSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPENYPQLTIRVSGY
                     AVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 ttctcattgg cagccttttc tccttgctct gcaaagatgc ggcgtatttc ttcgactttt
       61 tcccggtagg taaaactgcc gatagtggac actatttctt cttcggtcat ctctgctatt
      121 gcgtagtaga aaccttgaaa tatggtgaaa tgtttcattt ttattttgat tattgtttgt
      181 cgatgcaaag gaacttcttt gcggaaatgt gtaaaggaac tttgtataaa atggcaataa
      241 gttactacgg ttcgcccaaa aagtgtatga tagttgccac ttgtttcggc gaaaggagac
      301 ggttgcgtgc gcggtagcct tcctgttcga gttcgtgcag gagagggagg ttgtaaataa
      361 tccaacgacg aagtatttgt aaagcaactt taaggcatac atggggattg taaaggtgag
      421 ccagttctgc tttggtatag gtgcggataa ggaaagaatc ttctttgtaa ttcatgatgt
      481 tttcttcttg ttttatgcta taaagttaca taaaattcct tgttattcaa aggtttgact
      541 tcgtttttat tcgttgggaa tcaagaaact tcgttgggga atatggcttg tccggatacc
      601 cttatctttg tgctgttaac aagaagggga tgcatcctat gtattataaa ctttaaaatt
      661 attttattat ggcaatgaca gtatcttatt cagtggtgcc gcgcaagaat cctgcaaaaa
      721 agagcgaacc ggcaaagtat tatgcacagg cgcaggcgtc gggtgagctg gactttgaag
      781 agttgtgcga agcgatcacc agtcgttcta cttgtacgga aacggatgta cgtgcggcta
      841 tttcgggtat tctttacgaa gtgaaacgtg cgttgaaggc aggaagaatt gcgagactgg
      901 gcgatttggg cagtttgcag ataggactga acagtgaagg tgcggcatcg gtcaaggaat
      961 tttccggttc aatgattaag ggggcgcatc ttattttccg ccccggtaag acgctggcgg
     1021 agttgatgaa gattctgagt tatcagcaag tgcagactcg tgcggtggcg caggcgggtg
     1081 caggcgatgg cgggggagaa gaccctgaca agaatccgga ttccggtggt gatggctccg
     1141 gtgatgaaga ggctccggac cctacggtat aattggcaga cgggtcaagg taataacgaa
     1201 ttagaataac ttccattaat attttgataa gatgaaagaa atagtaagtc ggattttaga
     1261 tgtgatcatg tatctgattc ctttcttcgg aaagagaaag cgggacaggg tggtgagaga
     1321 agtgcgctat catactactt gtaaggaggt gtgcaaagtg aaaacgacgg aaagggaaaa
     1381 ggaacatgag aaagattagt ctgattgtga tccattgttc cgccacccgt gtcgattgcg
     1441 actttacggc aaaggatgtg gatacggctc accgttatcg tggcttttcg tgctgggggt
     1501 atcattatta tatacgcaag tcgggcgaga ttgagccgat gcgggatgaa gatacggtcg
     1561 gagctcatgc acgtggctat aatgcgatca gcttaggtgt atgttatgag ggcggtctgg
     1621 atgagaatgg aaaagctgcg gatacgcgta caccccgtca gaaggaggcg ctacatcgct
     1681 tggtacacga attgttgcag cgttatccgg aagctaaagt agtagggcat cgggatttaa
     1741 gtcccgacac caactataat gggatcgtag atccttggga acggattaaa gaatgccctt
     1801 gctttgaggt cattggagaa ttctccatta attaactacc accgtagtga aatactcttt
     1861 gaatacctcc gcagcttttt caagctgttc gggggtattt tcttttaccc ctttcatctt
     1921 gtattcctgt cccatcgctt cgtatttatg tacgcccagt gtatggtaag gcagtatttc
     1981 cacccgttgg atcatcttgt attttccgag tgcttctccc agtgcgcgta tgtcttcttc
     2041 aaaatcgctg tatccgggca ctagcacgta gcgtagccag aaaggtttct cctgttcttc
     2101 cagccaggcg gccgtgcgga tggtttgctc gttgcttctt ccggtgagcg tctgatggcg
     2161 gttgggattg aattccttta tatccaataa cacaagatcg gtcagtctga ataattcttc
     2221 tacgtcttcg ttccaaagtc ctccgttgct gtccagacag acatggatgc ctttttcttt
     2281 cagttcgcgg accagcggga cgagtgcttt cgcctgaaac gtcggttcgc ctccggagaa
     2341 agtgattcct cctcgtttcc cgaagaaggg gcgctggctc attgccatgc ggacgatttc
     2401 ttccggtggg gtaggcgtgc ctccctttcc tgctatcgta tccggattgg cgcaataggg
     2461 gcagcggaag ttgcatcctt gaagaaaaac gacgagccgt aagcccggcc cgtcgaatgt
     2521 tcccatactt tcgtatgaat gtacgtttat catcatatag agtaatagat tacatacgtt
     2581 cgtggaagct acggctgata acttccagtt ggtgttcgcg gctcagcttc acgaagttta
     2641 cggcatagcc ggaaacgcgg atagtcagct gcggatagtt ttccggatgc tccatggcat
     2701 cgtacagcat atcacggttc agtacgttta cgttcaggtg gtgggcacct ttggtgaagt
     2761 aaccgtccat catcgttacg aggttctcga tacggtcttc gtccgtagca cccagtgatt
     2821 tcggaacgat agagaaggtg ttgctgatac cgtcctgtga gtcacggtaa cgaagtttcg
     2881 ctacagaact cagagaagcg atcgcaccat tcttgtcgcg tccgtgcatc ggattggcac
     2941 ccggagcgaa ggcaacacct ttggcacgtc cgtcgggagt agcaccggtc tttttgccgt
     3001 acatcacatt ggaagtgata gtcagcaagg agagggtagg acgggcattc ttgtaaactg
     3061 gcaatttctt caattcttcg ctgaagaagt ataccaggtc tacgccaagg tggtctacct
     3121 tgtcgttgtc gttaccgaag caagggaatt ctccttcgat gtcgaagcct tctgtcagac
     3181 cgatatcatt gcggcgggca gttactttcg catatttgat ggcagacagt gaatcgaggg
     3241 cgatggaaag tccggctaca ccgtaagcca ggttgatgcg cgggttcgta tctacaagag
     3301 ccatctgggc cttctcgtag taatacttgt cgtgcatata gtggatgatg ttcatcgctt
     3361 cgttatatac gcgggcgatt tcggtcagca cttttttgta gttgtccatt acttcttcga
     3421 acttcagcgt gtcgctggtc agcacgggga tgttcttcac catgactgta ccggtattct
     3481 cgcaacgtcc gccgttgatg gcaagcaaca gggctttggc caggttgcaa cgggcgccga
     3541 agaactgaat ctgcttgcct atttcctggt atgatacgca gcaggcgatt ccgtagtcgt
     3601 cggactgacg tacttcgcgc atcaggtcgt cattttcgta ctggatagaa gaagtgtcga
     3661 tagaaacctt tgcacagaat tctttgaatc cttccggaag ttccgggctc cagagtacgg
     3721 tcaggttcgg ttccggtgaa gggccgaggt tgtacaatgt ctgcaggaaa cggaaggagg
     3781 tctttgtcac tttggtacgt ccgtcgttga gacgtccgcc cagagattcg gttacccagg
     3841 tcgggtcgcc tgcaaagatg tcgttgtatg attgcatacg caggtggcgg accatacgca
     3901 gtttgatgac aaactggtcg atcagctctt gcgcgaatga ttcggtgatg gttcctttgc
     3961 tcagttcata ttccagatag atatcgagga aggaggaaac gttacccagt gacatggcgg
     4021 caccgtcttg ttctttgacg gcggcaaggt aagccatata tacccactgt acggcttctt
     4081 gtgccgtgta agcaggacgg ctcaggtcga gaccgtagta ttcgcccatc actttcatgt
     4141 ctttcagtgc cttgatctgt tctgccactt cttcgcgcag gcggatgcgg gcctctgtca
     4201 tcggaccggt gaggttgtgc aaatcttcct tctttgcttc gatcagacgg tcgatgccgt
     4261 aaagagccat acggcggtag tcaccgatga tgcgtccgcg tgcataattg tcgggaagtc
     4321 cggtaaggaa tcccagcgaa cggaaggaac ggatttcttc cgtatatacg tcg
//
