LOCUS       c01014_gnlPro..         5636 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LDLFKFLK_1014.
ACCESSION   c01014_gnlPro..
VERSION     c01014_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 00:03:57
            Original ID  :: gnl|Prokka|LDLFKFLK_1014
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5636
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5636
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3704:5634]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3705..5634
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5636
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5636
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(2..2035)
                     /locus_tag="ctg1014_1"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GEKAANEKKKELPAIAFSASYRGRRTKVNLVKYLGHIVIDIDHLSKEELARILPIIKRC
                     DYTRIAFISPKGMGVKIIVRACHPDETLPETLQEIEDFHHAAYTRLVSFYTELCRIEID
                     TSGQDVARTCLFSYDPEIYFNPNADAFLVDQPQASYKISNRKNLSGSKQQTPPDGTPTN
                     EDTALNAHSANASLVLTLTYYHNKSEKYIAGNRNNYLHHLSCTFNRYGIPQEETSAFIK
                     SQFTDFPADETVSLINSAYAHTDEFNTCKLNGTQKRRLRIEQYISEHYETRYNEVLHIM
                     EYRRRRPDTEKPEPFRILDEMMENSIWIEINELGYPCTVKTIENLIYSDFSQSYHPIRE
                     YFELLPEWDGTDYIRILADSVQTSHQEFWAECLERYLVAMCAAATQENIVNHTVLLLCS
                     EIQNIGKTTFINNLLPPELRAYLSTGLINSNNKDDLAKITQAMLINLDEFEGMSGRELN
                     AFKDLVTRKVISFRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHPYSIQF
                     ITINYAQLYAQIKYLLNKPGYQYWFTQADNERLEENNEAFIFHSPEEEMVLTHIRKPER
                     FEKVYYLTVSEIAELIRERTGYQYSIGSKIQLGKVMTKHHFES"
     CDS             complement(2122..2352)
                     /locus_tag="ctg1014_2"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             2546..3049
                     /locus_tag="ctg1014_3"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLIFRPGKTLAELMKILSYQQVLTRAVAQAGAGDGGGEDPDKNPDSGGDGSGDEEAP
                     DPTV"
     CDS             3109..3276
                     /locus_tag="ctg1014_4"
                     /transl_table=11
                     /translation="MKEIVNRILDVIMYLIPFFGKRKRDKVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             3263..3712
                     /locus_tag="ctg1014_5"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(3705..4433)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73.7;
                     E-value: 6.1e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1014_6"
                     /sec_met_domain="Radical_SAM (E-value: 5.1e-31, bitscore:
                     97.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFKLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQGKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(4447..5634)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1014_7"
                     /sec_met_domain="Gly_radical (E-value: 1.5e-47, bitscore:
                     149.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 2.3e-156, bitscore:
                     510.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDL
                     MREVRQSDDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIP
                     VLTSDTLKFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINL
                     AYGVAGLSIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLV
                     YFFSEELKKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRD
                     KNGAIASLSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHH
                     LNVNVLNRDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 tagattcaaa atggtgctta gtcatcacct tccccaactg tatttttgat ccgatggaat
       61 actgataacc ggtacgctca cgtattagtt ccgcaatctc actgacagtc agataataaa
      121 ccttctcaaa acgttccggc ttgcggatat gcgtaagaac catttcttct tccggtgaat
      181 gaaagatgaa agcctcgtta ttctcttcaa gccgttcatt gtccgcttgc gtgaaccagt
      241 attggtaccc cggcttattc agcagatatt tgatctgagc atagagctgc gcataattga
      301 tggtaataaa ttggatggaa taaggatgaa aacaaaggaa acgccggttg cctgtcgtgt
      361 cgtgaagcac ttcctgataa ttgcaggttc ctgcaaaaga ggccgtatga gggaagtttt
      421 gcgaacggcg tgcatatggc agacggaaac tgatcacttt gcgcgttacc aaatctttga
      481 atgcattcag ttcccgaccg ctcatccctt caaactcgtc gagattgatc agcatggcct
      541 gcgttatttt ggctaaatca tctttattgt tcgaattgat tagtccggta gagagataag
      601 cacgcagttc gggaggcagg agattattga taaaggtagt ctttcctatg ttctgtatct
      661 cgctgcatag cagaagtacc gtgtgattga caatattctc ctgagtagcc gctgcgcaca
      721 tcgccaccag ataacgttcc aggcattccg cccaaaactc ctgatgactc gtctggacac
      781 tatccgccag gatacggata tagtcggttc cgtcccattc gggcagtaat tcaaaatact
      841 cgcgaatggg atgatacgac tggctgaaat cggagtaaat cagattttcg attgttttga
      901 cagtgcaggg atatcccagc tcgttgattt ctatccagat actgttttcc atcatctcat
      961 ccaagatgcg gaaaggctcc ggtttctcgg tgtcgggacg ccttctgcga tattccatga
     1021 tatgcagcac ttcattgtag cgtgtttcgt agtgctcgct gatatattgt tctatccgta
     1081 atcttcgttt ctgcgtgcca ttcagtttac aggtattaaa ttcgtcggta tgagcgtagg
     1141 cgctattgat caaagagact gtctcgtccg ccggaaagtc tgtaaactgg cttttgataa
     1201 aggcggatgt ttcctcctgc ggaatgccgt agcgattgaa ggtacatgag agatggtgca
     1261 gataattgtt ccggttgcct gctatgtatt tttcggactt gttgtgatag taagttaaag
     1321 tcaacaccaa agaagcgttg gcggaatggg cgttgagtgc tgtgtcttca ttcgtcgggg
     1381 tgccgtccgg aggagtctgt tgtttactgc cggagagatt tttacgattt gaaatcttgt
     1441 aggatgcctg aggttgatct acaagaaaag cgtcagcgtt cggattgaaa tagatctccg
     1501 gatcgtaaga gaacagacaa gtgcgtgcca catcctgacc ggaggtgtcg atttctatcc
     1561 gacagagttc ggtgtaaaaa gaaacgagtc tggtgtaagc tgcatgatgg aagtcttcta
     1621 tttcctgaag ggtttcgggc aatgtctcat ccggatggca tgcgcgcact atgattttca
     1681 cgcccatccc tttgggactg ataaaggcga tgcgggtgta atcgcatctc ttgatgatag
     1741 ggaggatgcg tgccaattct tctttgctga ggtggtcgat gtctatcacg atgtgtccca
     1801 gatatttgac gaggttcact ttggtacgcc ttcctcggta gctggcggag aaggcgattg
     1861 ccggcaattc ttttttcttc tcattggcag ccttttctcc ttgctctgca aagatgcggc
     1921 gtatttcttc gactttttcc cggtaggtaa aactgccgat agtggacact atttcttctt
     1981 cggtcatctc tgctattgcg tagtagaaac cttgaaatat ggtgaaatgt ttcattttta
     2041 ttttgattat tgtttgtcga tgcaaaggaa cttctttgcg gaaatgtgta aaggaacttt
     2101 gtataaaatg gcaataagtt actacggttc gcccaaaaag tgtatgatag ttgccacttg
     2161 tttcggcgaa aggagacggt tgcgtgcgcg gtagccttcc tgttcgagtt cgtgcaggag
     2221 agggaggttg taaataatcc aacgacgaag tatttgtaaa gcaactttga ggcagacatg
     2281 gggattgtaa aggtgagcca gttccgcttt ggtataggtg cggataagga aagaatcttc
     2341 tttgtaattc atgatgtttt cttcttgttt tatgctataa agttacataa aattccttgt
     2401 ttttcaaagg tttgacttca tttttattcg ttgggaatca agaaacttcg ttggggaata
     2461 tggcttgtcc ggctatcctt atctttgtgc tgttaacaag aaagggatgc atcctacgta
     2521 ttataaactt taaaattatt ttattatggc aatgacagta tcttattcag tggtgccgcg
     2581 caagaatcct gcaaaaaaga gtgaaccggc aaagtattat gcacaggcgc aggcgtcggg
     2641 tgagctggac tttgaagagt tgtgcgaagc gatcaccagt cgttctactt gtacggaaac
     2701 ggatgtacgt gcggctattt cgggtattct ttacgaagtg aagcgtgcgt tgaaggcagg
     2761 aagaattgcg agactgggtg atttgggtag tttgcagata ggactgaaca gtgaaggtgc
     2821 ggcatcggtc aaggaatttt ccggttcgat gattaagggg gcgcatctta ttttccgccc
     2881 cggtaaaacg ctggcggaat tgatgaagat tctgagttac cagcaagtgc tgactcgtgc
     2941 ggtggcgcag gcgggtgcag gcgatggcgg gggagaagac cctgacaaga atccggattc
     3001 cggtggtgat ggctccggtg atgaagaggc tccggaccct acggtataat tggcagacgg
     3061 gtcaaggtaa taacgaatta gaataacttc cattaatatt ttgataagat gaaagaaata
     3121 gtaaaccgga ttttagatgt gatcatgtat ctgattcctt tcttcggaaa gagaaagcgg
     3181 gacaaggtgg tgagagaagt gcgctatcat actacttgta aggaggtgtg caaagtgaaa
     3241 acgacggaaa gggaaaagga acatgagaaa gattagtctg attgtgatcc attgttccgc
     3301 cacccgtgtc gattgcgact ttacggcaaa ggatgtggat acggctcacc gttatcgtgg
     3361 cttttcgtgc tgggggtatc attattatat acgcaagtcg ggcgagattg agccgatgcg
     3421 ggatgaagat acggtcggag ctcatgcacg tggctacaat gcgatcagct taggtgtatg
     3481 ctatgagggc ggtctggatg agaatggaaa agcggcggat acgcgtacac cccgtcagaa
     3541 ggaggcgcta catcgcttgg tacacgaatt gttgcagcgt tatccggaag ctaaagtagt
     3601 agggcatcgg gatttaagtc ccgacaccaa ctataatggg atcgtagatc cttgggaacg
     3661 gattaaagaa tgcccttgct ttgaggtcat tggagaattc tccattaatt aactactacc
     3721 gtagtgaaat actctttgaa tacctccgca gctttttcaa gctgttcggg ggtattttct
     3781 tttacccctt tcatcttgta ttcctgtccc atcgcttcgt acttatgtac gcccagtgta
     3841 tggtaaggca gtatttccac ccgttggatc atcttgtatt ttccgagtgc ttctcccagt
     3901 gcgcgtatgt cttcttcaaa atcgctgtat ccgggcacta gcacataacg cagccagaaa
     3961 ggttttccct gttcttccag ccaggcggcc gtgcggatgg tttgctcgtt gcttcttccg
     4021 gtgagcgtct gatggcggtt gggattgaat tcctttatat ccaataacac aagatcggtc
     4081 agcttgaata attcttctac gtcttcgttc caaagtcctc cgttgctgtc cagacagaca
     4141 tggatgcctt tttctttcag ttcgcggacc agcgggacga gtgctttcgc ctgaaacgtc
     4201 ggttcgcctc cagagaaagt gattcctcct cgtttcccga agaaggggcg ctggctcatt
     4261 gccatgcgga cgatttcttc cggtggggta ggcgtgcctc cctttcctgc tatcgtatcc
     4321 ggattggcgc aatagaggca gcggaagttg catccttgaa gaaaaacgac gagccgtaag
     4381 cccggcccgt cgaatgttcc catactttcg tatgaatgta cgtttatcat catatagagt
     4441 aatagattac atacgttcgt ggaagctacg gctgataact tccagttggt gttcgcggct
     4501 cagcttcacg aagtttacgg cataaccgga aacgcggatg gtcagctgcg gatagttctc
     4561 cggatgctcc atggcatcgt atagcatatc acggttcagt acgtttacgt tcaggtggtg
     4621 ggcacctttg gtgaagtaac cgtccatcat cgttacgagg ttctcgatac ggtcttcgtc
     4681 cgtagcaccc agtgatttcg gaacgataga gaaggtgttg ctgataccgt cctgtgagtc
     4741 acggtaacga agtttcgcta cagagctcag agaagcgatc gcaccattct tatcgcgtcc
     4801 gtgcatcggg ttggcacccg gagcgaaggc aacacctttg gcacgtccgt cgggagtagc
     4861 accggtcttt ttgccgtaca tcacattgga agtgatagtc agcaaggaga gggtaggacg
     4921 ggcattcttg taaacaggca atttcttcaa ttcttcgctg aagaagtata ccaggtctac
     4981 gccgaggtgg tctaccttgt cgttgtcgtt accgaagcaa gggaattctc cttcgatgtc
     5041 gaaaccttct gtcaggccga tatcgttgcg gcgggcagtt actttcgcat atttgatggc
     5101 agacagtgag tcgagtgcga tggaaagtcc ggcaacaccg taagcaaggt ttatgcgcgg
     5161 gttcgtatct acaagagcca tctgggcctt ctcgtagtaa tacttgtcgt gcatatagtg
     5221 gatgatgttc atcgcttcgt tgtatacgcg ggcgatttcg gtcaacactt tcttgtagtt
     5281 gtccattact tcttcgaact tcagcgtgtc gctggtcagt acggggatgt tcttcaccat
     5341 gactgtaccg gtattctcgc aacgtccgcc gttgatggca agcaacaggg ctttggccag
     5401 gttgcaacgg gcaccgaaga actgaatctg cttgcctatt tcctggtatg atacgcagca
     5461 ggcgattccg tagtcatcgg actgacgtac ttcacgcatc aggtcgtcgt tttcgtactg
     5521 gatagaagaa gtgtcgatag aaacctttgc acagaattct ttgaatcctt ccggaagttc
     5581 cgggctccag agtacggtca ggttcggttc cggtgaaggg ccgaggttgt acaatg
//
