LOCUS       c00078_gnlPro..         6834 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GEGFMLIK_78.
ACCESSION   c00078_gnlPro..
VERSION     c00078_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 21:01:36
            Original ID  :: gnl|Prokka|GEGFMLIK_78
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6834
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..807)
                     /locus_tag="ctg78_1"
                     /transl_table=11
                     /translation="MKKKIFILLLCVVLIATCILCGVTGIFGNDSQNSPKPQTPDTAVT
                     ENTTFPSTVDSLTLKVGESKLIELSSEKAKSVTKWTSSDSKIVTVDDGGRVDALKEGTA
                     LISAISKDKSKSEFQVTVAKSTTKKQQSYSTCITANLDKLENNKRNTAKNLYAIKVNRT
                     ANCVTVYTYDEKGKYTIPVRAMICSTGLDNSTITGDYTIGIKSEWLFLVGDVFGRYISG
                     ISGDYLFHSVPYYSMSEEDLELEEFNKLGEQASQGCVRLAVSDAKWV"
     protocluster    1..6834
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2383:5376]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2384..5376
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6834
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6834
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             972..1508
                     /locus_tag="ctg78_2"
                     /transl_table=11
                     /translation="MVIIMAHPIKHFRTITRHRHKVIANCARAGILRQGLIHDLSKYSP
                     TEFIPGAKYFQGTRSPNEKERELYGYSQAWMHHKGRNRHHYEYWNDYNPKTKQIENVEM
                     PVKYVIEMFCDRVAASKIYNGKNYTDGAPFTYFCRIRGKHRMHPNTEALLEKLLVMLRD
                     EGEEKTFAYIRSMKK"
     CDS             1626..2117
                     /locus_tag="ctg78_3"
                     /transl_table=11
                     /translation="MKTDRVITAMIEYFGSDKKRIHHFLKVYSFAKTIGESENLLPDDQ
                     ELLEISAIVHDIGIKVSEEKYNSSAGKYQELEGPHEAEKLLAALGYEKTFIDKVCYLVG
                     HHHTYGNIDTLPYRILVEADFLVNLYEDDSSRSAAEQAYEKIFRTNTGKNLLKIMFLIP
                     "
     CDS             2384..4621
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg78_4"
                     /sec_met_domain="Gly_radical (E-value: 7.3e-42, bitscore:
                     131.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.1e-272, bitscore:
                     894.6, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREEWTNFAGGKWESDIDVRDFIQKNYTPYDGDQSFLEGPTEATD
                     KLWGELKELQKEERAKGGVLDMETEVVSGITAYGAAYLDKDLEKVVGLQTDKPLKRAFM
                     PYGGIKMAEQSCQMYGYTPSPKLHEIFTKYHKTHNQGVFDIYTPEMKLARHNKILTGLP
                     DTYGRGRIVGDYRRVALYGIDRLIEGKKEDFAETNRQGMRRGDFQLREEIADQIRALND
                     MKEMALSYGFDISQPAKNAAEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIYIERDL
                     ENGTLTESQAQELVDHFVMKCRMVKFARIESYNQLFSGDPVWATLEVAGLGIDGRSMVT
                     KNDYRFLHTLENMGPSPEPNLTVLYSSRLPENFKKYAAHISVTTSSIQYENDDVMRPVW
                     GDDYSICCCVSATQTGKEIQFFGARANLAKCLLYAINGGIDEKTKMQVGPEYTPITSEY
                     LDYDEVIKKYDTMMDWLAHLYVGTLNMIHYMHDKYYYEAAEMALIDTDVRRTFATGIAG
                     FSHVVDSLSAIKYAKVKVIRDEDGIAVDFETEGDFPRYGNNDERADKIAVDLLKTFMAK
                     LKYCHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQNGLLA
                     SLNSVAKLPYEYALDGISNTQTINPGALGHNDDERVDNLVNVMDGYFDQGAHHLNVNVF
                     GTEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVIARTCHDRM"
     CDS             4621..5376
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 76.2;
                     E-value: 1e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg78_5"
                     /sec_met_domain="Radical_SAM (E-value: 5.1e-31, bitscore:
                     97.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDNAVKGFVHSIETFGSVDGPGVRFVIFLSGCAMRCQFCHNPDT
                     WKHDENTVEYTADELLNKALAYRPYWKNKGGITVSGGEPLLQIDFLLDLFKKAKKQGVN
                     TCIDTSANPFTTKEPFYSKWKELMEYTDLLLLDIKHIDNEQHKILTGQPNTNILEMARQ
                     LSDMGKPVWIRHVLVPERNDKDEYLFRLADFIRSLKNVERVEVLPYHTLGVYKWKALGY
                     DYPLEGIEPPTKERVKNAEMILNGEKTRD"
     CDS             5894..6112
                     /locus_tag="ctg78_6"
                     /transl_table=11
                     /translation="MDKNKKLFYTIIGAISALAAATAAFLALKKHRCAEQKLIEEIDDI
                     VSEKEPEEAAKEEPQAEVTADELAEEE"
     CDS             6688..6834
                     /locus_tag="ctg78_7"
                     /transl_table=11
                     /translation="MKKIQKKLSVVLAVMMVLCMFTALPFSASAAETSEETSAGNKINV
                     TSNV"
ORIGIN
        1 aacccacttt gcatccgaaa ccgcaagtct tacgcatcct tgtgatgcct gttcgccaag
       61 cttgttgaac tcctcaagct ccaaatcttc ttcgctcatt gagtagtagg gaacggagtg
      121 gaaaagataa tcgcccgaaa ttccgctgat gtatcttccg aaaacatcgc ccacaaggaa
      181 aagccattcg gacttgattc cgattgtgta gtcacccgtg attgtcgaat tgtcaagacc
      241 tgtggagcaa atcatcgccc ttacggggat tgtgtacttg cccttttcgt catatgtgta
      301 aaccgtcacg cagtttgccg tgcggttgac cttgattgcg tacaggtttt ttgccgtgtt
      361 acgcttgttg ttttcaagct tgtcaagatt tgccgttata caggttgagt agctttgctg
      421 ttttttcgtg gttgattttg ccaccgtaac ctgaaattcc gactttgatt tgtcttttga
      481 aattgcagaa atcagagccg taccctcttt gagagcgtca acccttccgc cgtcatcaac
      541 ggtaacaatt ttactgtccg agcttgtcca tttggtaacg gattttgctt tttcggatga
      601 cagttcaata agctttgatt cgccgacttt taatgtgagc gaatcgaccg tgctcggaaa
      661 tgtcgtattt tcggttactg ccgtatcggg cgtctgcggt tttggagaat tttgagaatc
      721 atttccgaaa attcccgtca caccgcataa tatgcaggtt gcaatgagga caacacaaag
      781 taaaagaatg aaaattttct ttttcataga ttacctatcc ttatagaatt taacattaaa
      841 tggttacaaa attttaacct ttgtatatta taactttttt tcgacaatct tgcaagtgct
      901 attttaaatc ggcgagaaat gtggtaaaat gtcagtcgga gaatgttttg tattttccaa
      961 actaacaatt tatggtgata attatggcac atccgattaa acatttcaga acaataaccc
     1021 gtcacaggca caaggtaatt gccaactgtg caagggcagg aattctcaga cagggattaa
     1081 tacacgattt gtcaaagtat tcgcctacag aatttattcc gggtgcaaag tattttcagg
     1141 gcacgagaag tcccaatgaa aaggaaagag agctgtacgg ctattcacag gcgtggatgc
     1201 atcacaaggg cagaaaccgt caccattacg aatactggaa cgattacaac ccaaagacaa
     1261 agcagattga aaatgttgaa atgcctgtaa aatatgttat cgaaatgttc tgcgaccgag
     1321 ttgccgcaag caaaatctac aacggcaaaa attacacgga cggcgctccg tttacatatt
     1381 tttgcagaat cagaggcaaa caccgtatgc accccaacac cgaggctttg cttgaaaaat
     1441 tattggttat gctgagagat gagggtgaag aaaagacctt tgcatatata agaagtatga
     1501 aaaagtgatt gtttgtgcga atcctcccgt ctgaaatata gtcgcaaggc tacccctaca
     1561 gaattgcaca aacaaaaact atctacacga atagggtacg gcggctcgcc gtaaggagtg
     1621 ataatatgaa aaccgacagg gttataactg ctatgattga atattttgga agtgacaaga
     1681 aaaggattca ccattttctc aaggtttatt cattcgcaaa gacaatcggt gaaagtgaaa
     1741 atcttttgcc cgacgatcag gagcttcttg aaatctccgc aatcgttcat gatatcggca
     1801 taaaggtgag cgaagaaaag tacaattctt ctgcaggaaa gtaccaggaa cttgaaggcc
     1861 ctcatgaggc tgaaaagttg cttgccgctc tcggctacga aaaaacattt attgacaaag
     1921 tttgttacct tgtgggacac catcacacct atggaaatat tgatactttg ccgtaccgaa
     1981 ttttggtaga ggcagacttc cttgtgaacc tttacgagga cgattcaagc cgcagtgcag
     2041 cagagcaggc atatgagaaa attttccgca caaatacagg aaaaaatctg ctgaaaataa
     2101 tgttcctcat accctaaaat tgtactcatt taccatagtg ttttgagtgt gaaaaagtgg
     2161 tgtaaaaagt atctcaaaat gcttgacaac gcccttaaaa agtgttattt taatggtgtt
     2221 ggttatatga ttataaaagt gaataaatga cagaatacaa ggatgtatga cgattttttc
     2281 aaggagctga ccttaccggg cgtctgcttg gaattgagtc ggcatttttt tgtgtcatat
     2341 acaaaattca gtattaatta tcattatcgg aggttttaca gtaatgagag aagaatggac
     2401 aaattttgca ggtggcaaat gggagtccga cattgatgtc agagacttca ttcagaaaaa
     2461 ctacacacct tatgacggcg accagtcttt ccttgaaggt cctacagaag ctacagacaa
     2521 gctctggggc gagctcaagg aactccagaa ggaagaaaga gccaagggcg gcgttcttga
     2581 tatggaaact gaggttgtaa gcggcatcac agcttacggc gctgcatatc ttgataaaga
     2641 ccttgaaaag gttgtaggtc ttcagacaga caagcctctc aagcgtgcat ttatgcctta
     2701 cggcggtatc aaaatggctg agcagtcatg ccagatgtat ggctacactc caagccctaa
     2761 actccacgaa atctttacaa aatatcacaa gactcacaac cagggtgtat tcgatatcta
     2821 cactcccgag atgaaacttg cccgtcacaa caagattctt acaggtcttc ccgacacata
     2881 cggcagaggc agaatcgtcg gtgactacag aagagttgct ctttacggta ttgaccgtct
     2941 tatcgaaggc aagaaagagg actttgcaga aacaaaccgt cagggtatga gaagaggcga
     3001 cttccagctt cgtgaagaaa tcgctgatca gattcgtgct cttaacgata tgaaggaaat
     3061 ggcactttct tacggctttg atatttcaca gcctgctaag aacgctgcag aggcagttca
     3121 gtggctctac ttcggttatc ttgctgctat caagactcag aacggcgctg caatgagcgt
     3181 tggtcgtgta tcaacattcc tcgacatcta catcgagcgt gaccttgaga acggtacact
     3241 tacagaatca caggctcagg aactcgttga ccactttgtt atgaagtgcc gtatggttaa
     3301 gtttgcaaga attgagtctt acaaccagct cttctcgggc gaccctgtat gggctacact
     3361 cgaagttgca ggtcttggca ttgacggccg ttcaatggtt acaaagaacg actaccgttt
     3421 cctccacaca cttgagaata tgggaccttc acctgagcca aacctcacag ttctttattc
     3481 ttcaagactt ccggagaact tcaagaagta tgctgctcat atttcagtta caacaagctc
     3541 aattcagtac gagaacgatg atgtaatgcg tcctgtatgg ggcgatgatt actcaatctg
     3601 ctgctgcgta tccgcaacac agacaggtaa ggaaatccag ttcttcggcg caagagctaa
     3661 ccttgctaag tgccttctct acgctatcaa cggcggtatt gatgaaaaga caaagatgca
     3721 ggttggtcct gaatatacac ctatcacatc agagtacctt gattatgacg aagttatcaa
     3781 gaagtatgac acaatgatgg attggcttgc tcacctctat gtaggcacac ttaacatgat
     3841 tcactatatg catgataagt actactatga ggcagcagaa atggctctta tcgatacaga
     3901 tgtaagaaga acatttgcaa caggtattgc aggcttctca catgttgttg actcactcag
     3961 cgctattaag tacgctaagg ttaaggttat ccgtgacgag gacggcattg ctgttgactt
     4021 tgaaacagag ggcgacttcc caagatacgg taacaatgac gagagagcag ataagattgc
     4081 cgttgatctt ctcaagacat tcatggctaa gctcaagtat tgccacacat acagagattc
     4141 agagcctaca acttctatcc ttacaatcac atcaaatgtt gtttacggta aggctacagg
     4201 ttcacttcct gacggcagaa aagcaggcga gccgctttca ccgggtgcta acccaagcta
     4261 cggtgcagag cagaacggtc ttcttgcttc acttaactct gttgctaagc ttccttacga
     4321 gtacgctctt gacggtattt caaatactca gacaatcaac ccgggtgctc tcggacacaa
     4381 cgatgacgag cgtgttgaca acctcgtaaa tgttatggac ggctactttg atcagggcgc
     4441 acaccacctc aatgtaaatg tattcggtac agaaaaactc attgacgcta tggagcatcc
     4501 tgagaaggaa gagtacgcta acttcacaat ccgtgtatcg ggttatgctg ttaagttcat
     4561 cgaccttaca agagagcagc agctggatgt tattgccaga acctgccacg acagaatgta
     4621 atgtcggaca acgctgttaa aggctttgta cactcaatag aaacttttgg ctctgttgac
     4681 ggtccgggcg tgagatttgt gatttttctg agcggttgcg caatgcgttg ccagttctgc
     4741 cacaatcccg acacctggaa acacgatgaa aacaccgtgg aatacaccgc cgacgagttg
     4801 cttaataaag cgcttgcata ccgcccctat tggaaaaata agggcggtat cacagtaagc
     4861 ggaggtgagc cgcttttaca gattgacttt ttgcttgatt tgttcaagaa ggctaaaaag
     4921 cagggcgtta atacctgtat tgacacaagc gccaacccgt ttacaaccaa agagccgttc
     4981 tattcaaagt ggaaagagct aatggaatat acggatttgc tgttgcttga tataaagcat
     5041 atcgacaacg aacagcacaa aatccttaca ggacagccaa acaccaatat tcttgaaatg
     5101 gcaagacagc tttccgatat gggcaagccc gtatggataa gacatgttct tgtgcctgaa
     5161 agaaatgata aggacgagta tcttttcaga cttgccgact ttatccgttc gctcaagaat
     5221 gttgagcgtg tagaggtgct tccgtatcac accctcggtg tgtacaagtg gaaggctctc
     5281 ggttatgact atcctctcga gggcattgag cccccgacaa aagagcgtgt taagaatgcg
     5341 gaaatgattc ttaacggcga aaaaacaaga gattaatttc ttatataaca acggcagtac
     5401 cgattttttt ggtactgccg tttttgtttt gatttttatt tgttattctg cttttgttgt
     5461 tttttcttca gcaaaattga tgaatcaaag gtgtattcaa agatttttga gccatccttg
     5521 ttgagaaatc tcagcgtcgg aagaaccgac tgctccgacg ggtgagcctg caactgtcgg
     5581 tacaacctct gtaaatccga cttcggcaac aggcggtgcc gcaacatcgg acagcactcc
     5641 ttcacagcct gataaaaacg gcagtgttca gacaggagtg acagcaacgg ctgttatgat
     5701 gcttgttgta atttcgggcg gagtgctttt tgcccttgca gagcgtaaaa agagaatata
     5761 acacactgac agccatttgt gcaaagatta tcagttgcct attgaaaaat ttgtaaaaat
     5821 gatataaaat agcaataagt agaaaaatac ggtttttccg ctgatttata ttaatactga
     5881 aagaggtgcc ggaatggata aaaacaaaaa attattctac acaatcatcg gtgcaatctc
     5941 tgcacttgcg gctgccacag cagcatttct cgctttaaaa aagcacagat gcgcagaaca
     6001 gaagcttatt gaggaaattg acgatattgt ttccgaaaaa gagcctgagg aggctgcaaa
     6061 ggaagaaccg caggcagaag ttacagccga tgagcttgct gaggaagaat aagcaacaaa
     6121 ttgttgcacc caattcgggt agcagtattt gcatgtgcaa aagttaaata tctgagcggt
     6181 gctttcaatt aagaattgaa agttgaaaac agataatgat gtcggcggtg tgccaccgca
     6241 gtctattcaa gaaaacagtt taaaaaagaa aatgcatcag ttgcccgaaa aggtctgatg
     6301 catttttgtt ttgttaggct gttttgtcac agccccatcg aaattgctct tgacgcagac
     6361 ttttttgtta caaaacaatt gttgtaaaaa attaaatacg atagtattat tatcagttat
     6421 ttcagaactg aggaacatgg ataagacaat atccacaata atcagacaat gaatttgtat
     6481 ataatagcaa acttaccgtt aatttgcaca tattttaggg caaaaaattt tcgctaaaag
     6541 aataacagat tcattgcccg aacaacttaa atgtgctata atttaaatgt cctcggaaat
     6601 tttgtactgt taaagtttcc gaagctagaa gtattgccac aaaactgtgg cgaacggtaa
     6661 ttttatttta ttaaaggagc agcaagtatg aaaaaaattc agaaaaagct ttctgtggtt
     6721 cttgcggtaa tgatggtgct gtgcatgttc acagctctgc ctttctcagc aagtgctgcc
     6781 gaaacaagcg aagaaacaag tgcaggcaac aagatcaatg ttacatcaaa tgtg
//
