LOCUS       c00160_gnlPro..         4843 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HMNNPHKN_160.
ACCESSION   c00160_gnlPro..
VERSION     c00160_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 00:29:18
            Original ID  :: gnl|Prokka|HMNNPHKN_160
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4843
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4843
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1803:4843]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1804..4843
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4843
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4843
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..974)
                     /locus_tag="ctg160_1"
                     /transl_table=11
                     /translation="MDEWGKGNVNNNRQETKHLSRAVTYEGEALYRKEVNVPETFKGKR
                     LFLHVERTKKTTVFVDGKKIGSCDNVQTPHRYDLTEFLFPGKHTLTISVDNSRRHYPAG
                     VFNSHAFTEHTQTNWNGMLGKIFLEAVSDPFVESVKLVPEPEKKSVTVCLTIRNSYKPE
                     TARITLEAALQNNGNKGKVERKVIEVPLKLGDQHVKLNYPMGEEPELWSEFSPNLYKMH
                     IKLELAGKVDTTSKVFGMRCFAVQGTNFTINGKKIFLRGRHDACVFPLTGYPPMDKKAW
                     LRHLGIAAAYGFNYVRFHSWTPPEAAFEAADELGMYLQPEIPF"
     CDS             1804..4071
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg160_2"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-46, bitscore:
                     145.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 6.9e-283, bitscore:
                     927.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISIVKDLKESTALPKEWQGFKPGAWTESIDVRDFIQHNYTPYSG
                     NEEFLSGPSQRTLRLWDELKVLLKREIDNGGVLDADEEVVSSITSHKPGYIDKELEVVV
                     GLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYTSDI
                     RAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDRLIAERRKDLKNREHSPLTDELIRL
                     REEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGR
                     VSTFFDIYFTRDLEQGLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTES
                     IGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSS
                     VQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGK
                     QVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHD
                     PEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVD
                     DIACSLVSNFMEKLRKLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGA
                     NPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAY
                     FAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFH
                     TR"
     CDS             4088..4843
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg160_3"
                     /sec_met_domain="Radical_SAM (E-value: 2.2e-31, bitscore:
                     97.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSAGDVLREIARYRDFLQAAGGGVTLSGGDPLFQPDFTGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPERYRSLTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEGLSRHAALLGNVERVDVLPFHQMGRFKW
                     DELKLDYTLKGTPEASAKLAEQVRGIFRRE"
ORIGIN
        1 cagaatggta tttcaggctg aagatacatt ccaagttcat ccgcagcttc aaatgcagct
       61 tccgggggag tccatgaatg gaatcttaca taattgaatc cataggccgc cgctatacca
      121 agatggcgga gccatgcctt tttatccatg ggagggtatc cagtcagggg aaaaacgcag
      181 gcatcatgcc gacctcttaa aaaaatcttc ttcccattga ttgtaaaatt agttccctga
      241 acagcaaaac agcgcattcc gaagacttta ctggttgtgt cgacttttcc tgccaattcc
      301 agtttgatgt gcattttgta taaattcgga gagaattcgc tccagagctc cggctcttct
      361 cccatggggt aattcaattt cacatgttgg tctccgagtt taagaggaac ctcaatgact
      421 tttctctcca cttttccctt atttccgtta ttctgcaaag ccgcctccaa tgtaatacga
      481 gccgtttccg gcttataact gttgcgaata gtcaggcaaa cggtaacgga ttttttttct
      541 ggttcaggaa ccagcttaac cgattcaacg aagggatcag aaacggcctc aaggaatatt
      601 tttcccaaca ttccattcca attcgtctgg gtatgctcgg taaaagcgtg tgaattgaaa
      661 acgcctgccg gataatgcct gcggctgttg tccacagaga ttgtcaatgt atgtttcccc
      721 ggaaaaagaa attccgtcag atcataacga tggggtgttt gaacattatc acaggaaccg
      781 atttttttac catctacgaa aacggttgtc tttttcgtcc gttccacatg caaaaataac
      841 cgtttacctt tgaacgtttc cggaacattc acttcctttc tgtacaaggc ctctccttcg
      901 taagtaacgg cgcgggaaag atgttttgtc tcctgacggt tattattgac attccccttg
      961 ccccattcat ccatggttcc aggtagtaca acagcaactt tttcctgttt gaatggaagg
     1021 gaaagttccc ataacccatc caggggtagc aattctcgtc ccatcgctgg gaaaatagtc
     1081 ccccaaagca ggaaatatac agacatcatc cgaataagcc ataaaacatc ggaacaataa
     1141 ttggaaaaga agtatcttga ttttgttttc attataagaa tttgcatttg agaaaccgta
     1201 cggctcagtc attcgaccac ttgcacattg gaaatacgcc cttgtcacca ttgatttgag
     1261 agctccctaa aacatttggt tagtaatacg aagaactcct tccattttat caaacaacct
     1321 gaataccgtg acaacgaaag gcaaaaaagc atggattctt accgccgaca tgggaaacaa
     1381 ccacacctct ttgaatacgt tttgccgaaa actgtcagaa gagcttctga cagatatttt
     1441 cacaaaggta ttcattgata gctctaccgg aaaaacaatt gattggtact tcatcggcaa
     1501 ctcatttctt tgcttttttc ttttatatct tttcctattt ttctcgtttt cagatgagct
     1561 cccatgaaaa aagatgcccc cctacaagac tcttcagaag gccccattct gtttttccat
     1621 aaaaaatata catatctccg aaatgaacca gaaaaggtaa agaacagttc cctctcccag
     1681 aagaattagc cttgttgtct cccggaattc acgatataat tacgtggtgc ggagttttgc
     1741 cgtttccggc ctttcaacgg ctgcggaacg tttttctccg gtcttcttca ctacatccta
     1801 tttatgatca gcatagtaaa agacttgaag gagtccaccg cccttccgaa ggaatggcag
     1861 ggattcaagc cgggagcatg gacggagtcc attgacgtac gggatttcat ccagcataac
     1921 tacacgccct attccggcaa tgaagaattt ctttccggcc cctcccagcg cacgctccgt
     1981 ttatgggatg aactgaaagt cctgctgaaa agggaaatag acaacggcgg cgttctggat
     2041 gctgatgaag aagtggtatc ctccatcacc tcccacaagc ccggctacat tgacaaggaa
     2101 ctggaagtgg tcgtaggcct ccagacggac gcccccctga aacgggccct gatgcccttt
     2161 ggcggcctgc gcatggccca gcaggccctg gaatcctacg ggttcaagat gtgcgagaaa
     2221 accgcggata ttttcaaaaa gatacgcaag acgcacaatg aaggcgtttt cgacgcctac
     2281 acttccgata tccgggcggc ccgttccgcc ggcattatta ccggccttcc ggacgcctat
     2341 ggccgcggcc gcatcatcgg ggactaccgc cgggtggccc tgtacggcac ggacagactg
     2401 attgccgaac gccgcaagga cctcaaaaac cgtgaacatt ccccccttac ggatgagctg
     2461 atccgcctgc gcgaagaaat gagcgaacaa atacgcgccc tggaagaact cgcccagctg
     2521 ggcgcctcct acggctgcga cctcactcgc cccgccgcaa acgcccgcga agccgtgcaa
     2581 tggacgtacc tgggctacct ggccgccgtg aaggaacaga acggagcggc catgtccctg
     2641 ggccgcgttt ccactttctt cgacatttat ttcacccgcg acctggaaca ggggctcatc
     2701 acggaagagg aagttcagga aatcatcgac cagttcgtga tgaagctgcg catcgtccgt
     2761 ttcatccgca cgcctgatta caataacctg ttctccggag accccacctg ggtgacggaa
     2821 tccatcggcg gcatgggaga ggacgggcgc accctcgtca cccgcagttc cttccgcatg
     2881 ctgcagaccc tgtacaacct ggggccggct ccggaaccga acctgaccgt tctgtggtcc
     2941 cggaacctgc cggaagcctt caaaagcttc tgcgccaagg tttccattga aacgtcctcc
     3001 gtccaatacg agaacgacga cctgatgcgc ccgcactggg gcgacgacta cggcatcgca
     3061 tgctgtgtgt ccgccatgcg catcggcaag cagatgcagt tcttcggagc gcgcgccaac
     3121 ctcgccaagt gccttctcta cgccctgaac ggcggcgtgg atgaactcaa gggcaaacag
     3181 gtggccccgc cctccccccg ctacacggaa gaaattctca actatgatga agtgatggcc
     3241 ctgtacgaca agatgcagga ctggctcgcc aaaacttaca ttgacgcgct gaacatcatc
     3301 cactacatgc acgacaaata ctgctatgaa cgcattgaaa tggcgctgca tgatccggaa
     3361 atcctgcgca cgatggccac gggaatcgcc gggctttccg tggcggcgga ctccctgtcc
     3421 gccatcaagt acgccaccgt aaaagccatc cgcaatgaag aggggctgat tgtggacttc
     3481 aagacggaag gggaattccc ctgttacggg aacaatgacc cgcgcgtgga cgacatagca
     3541 tgcagcctgg tcagcaattt catggaaaaa ctgcgcaagc tccacactta ccgcaattcc
     3601 ctgcccaccc agtccatcct gacgatcacc tccaacgtgg tgtacggcaa aaagacgggc
     3661 aacacgccgg acggccgccg agccggggaa ccgttcgccc ccggcgccaa ccccatgcac
     3721 ggaagggaca ggaacggagc cgtggcgtcc atgctctccg tagccaagct gtcctatgac
     3781 gactccctgg acggcatctc ctacaccttc tccatcgttc cgcaggccct gggcaaggag
     3841 gaaagggaac gccgcgtcaa gctcgtctcc ctgctggacg cctactttgc cgctacaggt
     3901 caccacatta atgtgaacgt actggaacgg gaaacgcttc tcgacgccat ggatcacccg
     3961 gaaaaatacc cgcagcttac catccgcgtt tccggctatg ccgtgaattt catcaagctg
     4021 acccgggaac agcagcagga ggtcatcaac cgcaccttcc acacccgcta acccggacga
     4081 cacacggatg ccgcaggttt ttccacaaaa ccatgatccg gacggagaaa gctccgtcac
     4141 cggtttggtt cattccgtgg aatcctgcgg caccgtggac ggtcccggca tccgctttgt
     4201 ccttttcctg tccggctgca gcctgcgctg ccgctactgc cacaatccgg atacctccta
     4261 cgttcggcgg ggccggctca gaagcgcggg tgatgtcctc agggaaatag cccgttaccg
     4321 ggattttttg caggcggcgg gcggcggcgt caccctctcc ggcggggacc ccctcttcca
     4381 acctgatttt accggcgccg tgctgaaagg ctgccggaaa ctgggactgc atacctgcct
     4441 ggacacctcc ggccatctgg gcgtgaatgc gggcgaagaa atgctggcgg atacggacct
     4501 ggtcctgctg gatatcaagg catggaatcc ggaacggtac cgctccctga caggcgggga
     4561 actgcgccct acgctggagt ttgcggaacg cctggcctcc ctccgcaagc ccgtgtggct
     4621 gaggtatgtg ctggtacccg gcgttacgga taatctggaa gaaattgagg gactctcccg
     4681 ccatgcggcg ctcctgggca acgtggaacg cgtggacgtg ctccctttcc accagatggg
     4741 gagattcaaa tgggatgaat tgaagctgga ttataccttg aagggcaccc cggaagcgtc
     4801 cgcaaaactt gcggaacagg tccgcggcat tttccggcgg gaa
//
