LOCUS       c00124_gnlPro..         6828 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NMJLHOOH_124.
ACCESSION   c00124_gnlPro..
VERSION     c00124_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 01:52:09
            Original ID  :: gnl|Prokka|NMJLHOOH_124
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6828
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6828
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[896:6826]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      897..6826
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..6828
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6828
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             3..275
                     /locus_tag="ctg124_1"
                     /transl_table=11
                     /translation="DVHFIFYTSKSPTMKQKLIALSQTLAMIGRDDILIAAHQVSKDDK
                     ELLFRYGILAAFDKNTDVSDVSLTMLRVLVSMAKEEQNPTEEMPY"
     CDS             338..460
                     /locus_tag="ctg124_2"
                     /transl_table=11
                     /translation="MDSQKKMPGIAKNISEILSSTHQHTLSTAILHLYKHAKTI"
     CDS             complement(897..1304)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 60.3; E-value:
                     4.2e-19)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg124_3"
                     /sec_met_domain="Biotin_lipoyl (E-value: 6.8e-20, bitscore:
                     60.3, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKFTVTVNGQDYDVVVKDSGVAAAPAAAAPAAAPAPAAAPAPAA
                     APAPAPAPAAPAPAAAPAGAGEVVEAPMPGKVLRINKHVGDAVAAGDEVLVLEAMKMGN
                     PIMAPCDGKITGMPVSEGQSVQGGDTLFTVG"
     CDS             complement(1341..1643)
                     /locus_tag="ctg124_4"
                     /transl_table=11
                     /translation="MNNDTVMYIAVAAAVLAVIALIMICQVRSSLNQSAAPAAAAKKAA
                     PAAASRAADNGAVVAAIAAAIVAMGGGEIVSIRPAARPGWTTGARIAGVSGNQQF"
     CDS             complement(1686..2900)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg124_5"
                     /sec_met_domain="CT_Vparvula (E-value: 3.5e-213, bitscore:
                     698.0, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHAAKIVHVNELALKVGAPCVGLNDSGGARIQEAVDALSGYGKIF
                     WCNTIASGVIPQISAIMGPSAGGAVYSPALTDFIYMVKGTSQMFLTGPAVVESVTGEKI
                     TAEALGGAMTHNRTSGVAQFAAENDEDCIKQIRYLLSFLPSNNMEDAPIVETGDDPTRT
                     DASLDTIMPENSNAPYDMYDVIKSIVDNGEYYDVAKEWAKNIITCFCRMDGQTVGIIAN
                     QPNFMAGCLDYNASDKAARFIRFCDCFNIPVLNIVDVPGFLPGKQQEYAGVIRHGAKML
                     FAYCEATVPKITLILRKAYGGSYIAMCSREQGADQVFAWPTAEIAVMGPAGAANIIFKK
                     DPQKEQRTQEYVDEFATPYKAAERGYVDAVIDPRNSRPVVINALKMLASKHEVHPAKKH
                     GNIPL"
     CDS             complement(2902..3222)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg124_6"
                     /sec_met_domain="CT_Vparvula (E-value: 7e-27, bitscore:
                     83.6, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATVAERIELLHNNLAHVEAMGGEKKVEKQHAKGKLTARERLALL
                     FDEGSFVEVNALVKSRCYNFGQEKKDLPGEGVVTGYGTVDGRLVFAFXXXXGLHGRRRL
                     PW"
     CDS             complement(3296..3733)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg124_7"
                     /sec_met_domain="Glyoxalase_4 (E-value: 3.1e-16, bitscore:
                     49.1, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAFKVLCVDHVGVAVNDLALIKKQMKDLLNLEPSLPDETVEEQHV
                     TTSFFKPSAQSEACELEFLGSTTPDGPIARYIAKNGGKNGFQHVALRVDNIVEALAALP
                     EDAKLDKHWRVGAGGCHIAFMHPKATGLLLELTERPGGPSF"
     CDS             complement(3752..4690)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: MeaB"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg124_8"
                     /sec_met_domain="MeaB (E-value: 1.8e-103, bitscore: 334.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFSMKNFWDGSRRALARAITIVEDERDGWEDIMKEIYHHTGRAQ
                     VIGITGAPGAGKSTLSDALIKQYRKQGKKVGIVAIDPTSPFSGGAILGDRIRMNDLTLD
                     EGVYIRSMGTRGSLGGLSRKTTDAVKLMDAFGLDVIFIETVGVGQSEVDIVKNADTTLV
                     VLVPGLGDDIQAIKAGILEIGDVFCINKCDRDGADRLNVEIEMMLDLGEAQNWRPPIER
                     TIANKDEGVADVVKSLNSHRKYLEESGLLEERRRERARTEMVEMIHDRISRHVMEDIVQ
                     TGKFADYVEQVYERENDPFTVVDSIVGNIFK"
     CDS             complement(4793..5185)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: B12-binding"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg124_9"
                     /sec_met_domain="B12-binding (E-value: 3.1e-24, bitscore:
                     74.8, seeds: 91, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEKRIRVLVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQTV
                     AQIVEAADAEDVNVVALSLLSGAHNTLFPEVVEALKAKGMEDVLVIGGGVIPAGDIPGL
                     KKAGVKAIFTPGTPTKEIIDFIKANV"
     CDS             complement(5216..6826)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: MM_CoA_mutase"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg124_10"
                     /sec_met_domain="MM_CoA_mutase (E-value: 2.6e-225,
                     bitscore: 738.1, seeds: 314, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="YEAACAKSYAKTPERVGLKHNKVYTPLDIEGFDYERDLGIPGEYP
                     YTRGVQPTMYRGRLWTMRMYAGFATAEESNKRYRYLIENGGSGLSCAFDLPTQIGYDSD
                     DKMAIGEIGKVGVAIDTLKDMEILFDHIDLGKVSTSMTINAPASVLLAMYIAVAEKQGV
                     PADQLRGTIQNDILKEYAARGTYIFPVKPSMRLITNIFEYCSKNVPKWNTISISGYHIR
                     EAGSTAAQEIAFTIADGIAYIEAALKAGMKIDDFAGRLSFFWNAHNNVLEEVAKFRASR
                     RLWATILKERFHAQNPKSMKLRFHTQTAGSMLTAQQPNNNIVRVALQTAAAVMGGTQSL
                     HTNSRDEALALPTTESVTIALRTQQIVAYESGLADVVDPLGGSYYVEAMTNAIEAEAKE
                     YIRKIDEMGGAVEAIDKGYIQKEIQDSAYAWQMAVESGERTIVGVNKFTMEEAPVEGLL
                     KVDASAGEFQKNKLAKVKAERDNAKVKEELAKLEVAAADESVNLMPVILDCVRAYCSLG
                     EICGVLRKVFGEYKAHNTL"
ORIGIN
        1 atgacgtcca tttcatcttc tacacatcca aaagccccac gatgaagcaa aaattgatcg
       61 cgctctccca gacacttgcc atgatcggcc gggacgacat cctcatagcc gcccatcagg
      121 tcagcaaaga tgataaggaa ctcctcttcc gttacggcat tctcgccgct ttcgacaaga
      181 atacggatgt aagcgatgta agccttacca tgctccgcgt cctcgtttcc atggcaaaag
      241 aagaacagaa tcccaccgag gaaatgcctt attgattctc agtccctcat aaacaggaga
      301 aacatcctcc tgttttttta ttgcggacaa acagcatatg gactcccaaa agaaaatgcc
      361 gggcattgca aaaaatattt cagaaatact ttcatctact catcagcata ctttaagcac
      421 tgctatcctg catctgtaca aacatgcaaa aacgatttga tcccacagct ccgcgttttg
      481 gtccggcatc cggaaccgcc tcgataccac ctctccatgc agcttctgta taagcagatg
      541 caaatcaata ttcagcagtt tagcagatac ttattgcata attctattac gaccggagcc
      601 cttaaaacac aatcaaaaaa ttcttccggc atctcataat gcaaaacaac ctattctgta
      661 tgcccgactg tttcggattt tctatactca tcaagtaagc agaaatactg ctcatcatta
      721 tagtcagatt cttccccacc tccttacaaa gatgaatagt acctgcactt taatatgata
      781 tctgcaatcc aggaatctct aaaaatctct ttttgccttt aatgaaatcc attatcccgt
      841 tcacaaaatt aaacagcaaa aaagcagtgc cgaagcactg ctttttgtaa ttagaatcag
      901 ccaactgtga agagtgtgtc gccgccctgt acgctctggc cttcgctgac cggcataccg
      961 gtaatcttgc catcgcaggg agccatgatc ggattaccca tcttcatagc ttcgagaacg
     1021 agaacttcgt cgcctgccgc tactgcgtca ccaacgtgtt tattgatacg caggactttg
     1081 cccggcatcg gagcttctac gacttcacct gcgccggcgg gtgctgctgc cggtgcgggt
     1141 gctgctggag caggtgccgg agccggagct gctgccggtg caggagctgc tgccggtgcg
     1201 ggtgctgccg ccggtgccgc tgctgcgggg gctgctgcta cgccgctgtc tttaactact
     1261 acatcgtaat cctggccgtt aactgttacg gtaaattttc tcattgttct tcctcctaat
     1321 tttgtacttt caactttcaa ttagaactgc tggtttccgg aaacacctgc aatgcgggcg
     1381 cctgtggtcc agccaggtct tgctgccggg cggatagaaa cgatttctcc gccgcccata
     1441 gcaacgattg ctgctgcaat ggctgctaca acagcaccat tatcagcggc tctcgaagcc
     1501 gctgccggcg ccgccttttt agctgctgcc gccggtgctg cggactgatt gagagatgaa
     1561 cgtacttggc agatcatgat cagggcaatg actgccagaa cggcagctgc taccgcgata
     1621 tacataactg tatcgttatt catccaaacc catcctttca aatattatca atgtgtaaac
     1681 gtgtattaca gcggaatgtt tccgtgtttc ttagccggat gtacttcatg tttggaagca
     1741 agcatcttca gagcattgat aactaccgga cgagagtttc tcggatcaat aactgcgtct
     1801 acatagccgc gttctgcagc tttgtaagga gttgcaaact catcaacata ctcctgtgta
     1861 cgctgttctt tctgcggatc cttcttgaaa atgatatttg ctgcgccggc aggtcccata
     1921 acggcgattt ctgcggtcgg ccatgcaaat acctggtcag ccccctgttc acgggagcac
     1981 atggcgatgt aagaaccacc gtatgcttta cggagaatca gggtaatctt cggtacggtt
     2041 gcctcgcagt atgcgaacag catcttagca ccgtgacgga ttacacctgc atattcctgc
     2101 tgcttgccgg gcaggaagcc gggaacgtcg acaatgttca gaaccggaat attgaagcag
     2161 tcgcagaaac gaataaagcg ggcagcttta tcagaagcat tatagtcaag gcagcctgcc
     2221 atgaagttcg gctggttggc aataatgccg acagtctgtc catccatacg gcagaagcag
     2281 gtaatgatgt tcttagccca ttccttagct acgtcgtagt actcgccgtt atcaacgatg
     2341 gatttgatga catcgtacat atcgtacggt gcattggagt tttccggcat aatggtatcc
     2401 agacttgcat ctgtacgggt cggatcatct ccggtttcaa caatcggcgc gtcttccatg
     2461 ttgttgctcg gcaggaagga aagcaggtaa cgaatctgtt taatgcagtc ttcgtcattt
     2521 tcagctgcaa actgggccac accggatgta cggttatggg tcatagcgcc gccgagagct
     2581 tcagcggtaa tcttttcgcc ggttacggat tcaactactg ccggtcctgt caggaacatc
     2641 tgagaggtgc cttttaccat gtagatgaag tcggtcagtg ccggagaata tacggcgccg
     2701 cctgcagatg gtcccatgat agcagagatc tgcggaataa cgccggatgc aatggtattg
     2761 caccagaaaa tcttgccata gccggaaaga gcatctactg cttcctggat acgggctccg
     2821 ccggaatcat tcaggcctac gcaaggagca ccgactttaa gagccagttc gtttacgtga
     2881 acaattttcg cagcgtgcat ttcaccaagg gagccgcctt ctaccgtgaa gtcctgnnnn
     2941 nnnnnnaaat gcgaatacga gtcttccgtc tacagtacca taaccggtaa cgacaccttc
     3001 gccggggaga tctttcttct cctggccgaa gttgtagcag cgggacttaa caagcgcatt
     3061 gacttctaca aaagagcctt catcaaacag aagagcaagt ctttcgcggg ctgtcagttt
     3121 gccttttgca tgctgttttt caactttctt ttctccaccc atagcctcta cgtgtgcgag
     3181 gttattgtga agaagttcaa ttctttctgc aacagttgcc attcttacac ctccaaaaat
     3241 attggtccct taccgggacg tccctattat agcataggga ccgatcctta tgctattaga
     3301 aagacgggcc gccggggcgt tcggtcagtt ccagcagaag gccggtagct ttcgggtgca
     3361 taaatgcgat gtggcagccg ccagcaccaa ctctccaatg cttgtcaagc tttgcgtctt
     3421 ccggaagtgc agcaagcgct tcgacaatgt tgtctacgcg gagagctaca tgctggaaac
     3481 cgttcttgcc accgttctta gcgatatagc gggcaatcgg gccgtccgga gttgtggacc
     3541 ccaggaattc cagttcgcat gcttcgctct gtgcggacgg tttgaagaag cttgtcgtta
     3601 catgctgttc ttctaccgtt tcatccggaa gggacggttc aaggttcagc agatctttca
     3661 tctgcttctt gatcagcgcc agatcattaa cagctacgcc gacatggtct acacataata
     3721 ctttgaatgc cataataatt cctcctttga attatttaaa tatatttccc acgatagaat
     3781 caacgactgt gaaaggatca ttttctcttt catagacttg ttctacgtag tcagcaaatt
     3841 tacccgtctg tacgatatct tccataacat gacgggaaat gcggtcgtga atcatctcta
     3901 ccatctcagt gcgggcgcgt tcacgtctgc gttcttccag caagccggat tcttcaagat
     3961 atttacgatg ggaattaaga gatttgacaa cgtccgcgac gccttcgtcc ttgtttgcaa
     4021 tcgtcctttc aataggagga cgccagtttt gggcttcacc caaatcaagc atcatttcga
     4081 tttcaacatt cagacggtcc gcgccgtcac gatcacattt attaatgcag aatacatccc
     4141 cgatttcaag gatgcctgcc ttaatagcct gaatatcgtc acccagtccg ggtacgagca
     4201 caacgagtgt tgtatccgca ttcttgacga tatctacttc tgactgccct acgcctactg
     4261 tttcgatgaa aatgacatcg agaccgaaag catccatcag ttttaccgca tcggtagtct
     4321 ttctggacaa accgccaaga ctgccgcggg tacccatact gcggatatat acaccttcgt
     4381 ccagtgtgag gtcattcatt ctgatacggt cgccaaggat agcaccgccg gagaatggag
     4441 aagtcggatc gatagccaca ataccgactt tcttaccctg ttttctgtac tgtttaatca
     4501 gcgcgtctga aagtgtgctc ttacccgcac caggtgcacc agtgatgccg atgacctgtg
     4561 cacgaccggt atggtggtag atttctttca tgatatcttc ccagccatca cgttcgtctt
     4621 caacgatggt tattgctctc gctaaggcac gtctggaacc gtcccaaaaa tttttcattg
     4681 agaattccat ggatacaccg cctttttaag agtaaataat agcggcatgg ggccacagca
     4741 aatgcagccc cgtgcaacta tcgagcttct attggataga agagaatgtc aattatacgt
     4801 tagctttgat aaagtcgata atttccttgg tcggtgtgcc aggtgtaaag atagctttta
     4861 cgccggcctt tttcaaacca gggatgtcac cggcagggat tacgccgccg ccgataacga
     4921 gaacatcttc cattccttta gctttcagtg cttctaccac ttccgggaac agggtgttgt
     4981 gagcgccgga caaaaggctc agtgctacaa cattaacgtc ttccgcatct gccgcttcaa
     5041 cgatctgagc gacagtctgg cgaagacctg tgtaaataac ttccatacct gcatcgcgaa
     5101 gtgcgcgggc aactactttt gcaccgcgat catgtccatc aagacccggt ttagcaacga
     5161 gtactctgat acgtttttct gccatagtta tatacctcca gaattttagt taggattaaa
     5221 gtgtgttatg tgctttgtat tcgccgaata ctttgcggag aacgccgcaa atttcgccga
     5281 gagagcagta agcacgtacg cagtcaagga taacaggcat caggttgacg gattcgtcag
     5341 cagctgcaac ttccagcttg gccagttctt ccttaacctt tgcgttatca cgttctgcct
     5401 taactttagc cagtttgttc ttctggaatt cgccggcgga tgcgtcaacc ttcaggaggc
     5461 cttcaaccgg agcttcttcc atagtgaact tgttgacgcc gacaattgtt ctttcgccgg
     5521 attcaacagc catctgccaa gcataagcag aatcctggat ttctttctgg atataacctt
     5581 tatcgatagc ttcaacagcg ccgcccattt catcaatctt gcggatgtat tctttagctt
     5641 ccgcttcgat ggcgttggtc atagcttcta cataatagga gccgcccagc ggatcgacta
     5701 cgtcagccag accagattca taagcaacga tctgctgtgt acgcagagcg atggttacgg
     5761 attcagtagt cggcagagca agagcttcat cgcgggagtt tgtatgcagg gactgtgttc
     5821 cgcccataac tgccgctgct gtctggagag caacacgaac gatgttgttg ttcggctgct
     5881 gagctgtcag catggagccg gctgtctggg tatggaaacg cagtttcatg gacttcgggt
     5941 tctgagcatg gaaacgttct ttcagaatcg tagcccagag acgacgggat gcacggaatt
     6001 tagcaacttc ttcaagtacg ttgttgtgag cgttccagaa gaaggacaga cggcctgcaa
     6061 agtcgtcgat cttcatgcct gctttcagag ctgcttcgat gtaagcaata ccatcagcaa
     6121 tggtgaaagc gatttcctga gcagcggtgg aacctgcttc acggatatgg tagccggaga
     6181 tagaaatggt gttccacttc ggtacattct tggagcagta ttcaaaaatg ttagtaatca
     6241 aacgcataga cggtttaacc gggaaaatat aggttccgcg ggctgcgtat tctttcagaa
     6301 tatcattctg gatagtgccg cggagctgat cagccggtac gccctgcttt tcagcgactg
     6361 caatgtacat agccagcagt acagatgccg gtgcgttgat ggtcatggat gtagaaacct
     6421 tgcccagatc aatatggtcg aaaaggattt ccatatcttt cagggtatca atggctacac
     6481 ctaccttgcc gatttcgccg atagccattt tgtcatcaga gtcatagccg atctgggtcg
     6541 gaaggtcaaa tgcacaggac aggccggaac cgccgttttc aatcaggtaa cgataacgct
     6601 tgttggattc ttcagcggta gcgaagcctg catacatacg catggtccag agacggccgc
     6661 ggtacatggt cggctgtacg ccgcgggtat acggatattc gcccgggatg ccaaggtctc
     6721 tttcatagtc aaaaccttcg atgtcaagag gtgtatacac cttattgtgt tttaaaccaa
     6781 ctctttccgg agtctttgcg taagatttag cgcaagcagc ttcgtatg
//
