LOCUS       c00096_gnlPro..         3669 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BFLOGHPP_96.
ACCESSION   c00096_gnlPro..
VERSION     c00096_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 00:30:44
            Original ID  :: gnl|Prokka|BFLOGHPP_96
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3669
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..294
                     /locus_tag="ctg96_1"
                     /transl_table=11
                     /translation="NSSAGKYQEIEGPHEAEKLLAALGYEKTFIDKVCYLVGHHHTYGN
                     IDTLPYRILVEADFLVNLYEDDSSRSAAEQAYDKIFRTNTGKNLLKIMFLIP"
     protocluster    1..3669
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[560:3553]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      561..3553
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3669
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3669
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             561..2798
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg96_2"
                     /sec_met_domain="Gly_radical (E-value: 3.1e-42, bitscore:
                     131.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.7e-273, bitscore:
                     894.6, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREEWTNFAGGKWESDIDVRDFIQKNYTPYDGDQSFLEGPTEATD
                     KLWGELKELQKEERAKGGVLDMETEVVSGITAYGAAYLDKDLEKVVGLQTDKPLKRAFM
                     PYGGIKMAEQSCQMYGYTPSPKLHEIFTKYHKTHNQGVFDIYTPEMKLARHNKILTGLP
                     DTYGRGRIVGDYRRVALYGIDRLIEGKKEDFAETNRQGMRRGDFQLREEIADQIRALND
                     MKEMALSYGFDISQPAKNAAEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIYIERDL
                     ENGTLTESQAQELVDHFVMKCRMVKFARIESYNQLFSGDPVWATLEVAGLGIDGRSMVT
                     KNDYRFLHTLENMGPSPEPNLTVLYSSRLPENFKKYAAHISVTTSSIQYENDDVMRPVW
                     GDDYSICCCVSATQTGKEIQFFGARANLAKCLLYAINGGIDEKTKMQVGPEYTPITSEY
                     LDYDEVIKKYDTMMDWLAHLYVGTLNMIHYMHDKYYYEAAEMALIDTDVRRTFATGIAG
                     FSHVVDSLSAIKYAKVKVIRDEDGIAVDFETEGDFPRYGNNDERADKIAVDLLKTFMAK
                     LKYCHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQNGLLA
                     SLNSVAKLPYEYALDGISNTQTINPGALGHNDDERVDNLVNVMDGYFDQGAHHLNVNVF
                     GTEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVIARTCHDRM"
     CDS             2798..3553
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 76.2;
                     E-value: 1e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg96_3"
                     /sec_met_domain="Radical_SAM (E-value: 2.2e-31, bitscore:
                     97.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDNAVKGFVHSIETFGSVDGPGVRFVIFLSGCAMRCQFCHNPDT
                     WKHDENTVEYTADELLNKALAYRPYWKNKGGITVSGGEPLLQIDFLLDLFKKAKKQGVN
                     TCIDTSANPFTTKEPFYSKWKELMEYTDLLLLDIKHIDNEQHKILTGQPNTNILEMARQ
                     LSDMGKPVWIRHVLVPERNDKDEYLFRLADFIRSLRNVERVEVLPYHTLGVYKWKALGY
                     DYPLEGINPPTKERVKNAEMILNGEKTRD"
ORIGIN
        1 aattcgtctg caggaaagta tcaggagatt gaaggtcctc acgaggctga aaagttgctt
       61 gccgctctcg gctacgaaaa aacatttatt gacaaagttt gttaccttgt gggacaccat
      121 cacacctatg gaaatattga tactttgccg taccgaattt tggtagaggc tgacttcctt
      181 gtgaaccttt acgaggacga ttcaagccgc agtgcagcag agcaggcata tgacaaaata
      241 ttccgcacaa atacgggaaa aaatctgctg aaaataatgt tcctcatacc ctaaaattgt
      301 actcatttac catagtgttt tgagtgtgaa aaagtggtgt aaaaagtatc tcaaaatgct
      361 tgacaacgcc cttaaaaagt gttattttaa tggtgttggt tatatgatta taaaagtgaa
      421 taaatgacag aatacaagga tgtatgacga ttttttcaag gagctgacct taccgggcgt
      481 ctgcttggaa ttgagtcggc atttttttgt gtcatataca aaattcagta ttaattatca
      541 ttatcggagg ttttacagta atgagagaag aatggacaaa ttttgcaggt ggcaaatggg
      601 agtccgacat tgatgtcaga gacttcattc agaaaaacta cacaccttat gacggcgacc
      661 agtctttcct tgaaggtcct acagaagcta cagacaagct ctggggcgag ctcaaggaac
      721 tccagaagga agaaagagcc aagggcggcg ttcttgatat ggaaactgag gttgtaagcg
      781 gcatcacagc ttacggcgct gcatatcttg ataaagacct tgaaaaggtt gtaggtcttc
      841 agacagacaa gcctctcaag cgtgcattta tgccttacgg cggtatcaaa atggctgagc
      901 agtcatgcca gatgtatggc tacactccaa gccctaaact ccacgaaatc tttacaaaat
      961 atcacaagac tcacaaccag ggtgtattcg atatctatac tcccgagatg aagcttgccc
     1021 gtcacaacaa gattcttaca ggtcttcccg acacatacgg cagaggcaga atcgtcggtg
     1081 actacagaag agttgctctt tacggtattg accgtcttat cgaaggcaag aaagaggact
     1141 ttgcagaaac aaaccgtcag ggtatgagaa gaggcgactt ccagcttcgt gaagaaatcg
     1201 ctgatcagat tcgtgctctt aacgatatga aggaaatggc actttcttac ggctttgata
     1261 tttcacagcc tgctaagaac gctgcagagg cagttcagtg gctctacttc ggttatcttg
     1321 ctgctatcaa gactcagaac ggcgctgcaa tgagcgttgg tcgtgtatca acattcctcg
     1381 acatctacat cgagcgtgac cttgagaacg gtacacttac agaatcacag gctcaggaac
     1441 tcgttgacca ctttgttatg aagtgccgta tggttaagtt tgcaagaatt gagtcttaca
     1501 accagctctt ctcgggcgac cctgtatggg ctacactcga agttgcaggt cttggcattg
     1561 acggccgttc aatggttaca aagaacgact accgtttcct ccacacactt gagaatatgg
     1621 gaccttcacc tgagccaaac ctcacagttc tttattcttc aagacttccg gagaacttca
     1681 aaaagtatgc tgctcatatt tcagttacaa caagctcaat tcagtacgag aacgatgatg
     1741 taatgcgtcc tgtatggggc gatgattact caatctgctg ctgcgtatcc gcaacacaga
     1801 caggtaagga aatccagttc ttcggcgcaa gagctaacct tgctaagtgc cttctctacg
     1861 ctatcaacgg cggtattgat gaaaagacaa agatgcaggt tggtcctgaa tatacaccta
     1921 tcacatcaga gtaccttgat tatgacgaag ttatcaagaa gtatgacaca atgatggatt
     1981 ggcttgctca cctctatgta ggcacactta acatgattca ctacatgcat gataagtact
     2041 actatgaggc agcagaaatg gctcttatcg atacagatgt aagaagaaca tttgcaacag
     2101 gtattgcagg cttctcacat gttgttgact cactcagcgc tattaagtac gctaaggtta
     2161 aggttatccg tgacgaggac ggcattgctg ttgactttga aacagagggc gacttcccaa
     2221 gatacggtaa caatgacgag agagcagata agattgccgt tgatcttctc aagacattca
     2281 tggctaagct caagtattgc cacacgtaca gagattcaga gcctacaact tctatcctta
     2341 caatcacatc aaatgttgtt tacggtaagg ctacaggttc acttcctgac ggcagaaaag
     2401 caggcgagcc actttcacct ggtgctaacc caagctacgg tgcagagcag aacggtcttc
     2461 ttgcttcact taactctgtt gctaagcttc cttacgagta cgctcttgac ggtatttcaa
     2521 atactcagac aatcaacccg ggtgctctcg gacacaacga tgacgagcgt gttgacaacc
     2581 tcgtaaatgt tatggacggc tactttgatc agggcgcaca ccacctcaat gtaaatgtat
     2641 tcggtacaga aaaactcatt gacgctatgg agcatcctga gaaggaagag tacgctaact
     2701 tcacaatccg tgtatcaggt tatgctgtta agttcatcga ccttacaaga gagcagcagc
     2761 tggatgttat tgccagaacc tgccacgaca gaatgtaatg tcggacaacg ctgttaaagg
     2821 ctttgtacac tcaatagaaa cttttggctc tgttgacggt ccgggcgtga gatttgtgat
     2881 ttttctgagc ggctgcgcaa tgcgttgcca gttctgccac aatcccgaca catggaaaca
     2941 cgatgaaaac accgtggaat acaccgccga cgagttgctt aataaagcgc ttgcataccg
     3001 cccctattgg aaaaataagg gcggtatcac agtaagcgga ggtgagccgc ttttacagat
     3061 tgactttttg cttgatttgt tcaagaaggc taaaaagcag ggcgttaata cctgtattga
     3121 cacaagcgcc aacccgttta caaccaaaga gccgttctat tcaaagtgga aagagctaat
     3181 ggaatatacg gatttgctgt tgcttgatat aaagcatatc gacaacgaac agcacaaaat
     3241 ccttacagga cagccaaaca ccaatattct tgaaatggca agacagcttt ccgatatggg
     3301 caagcccgta tggataagac atgttcttgt gcctgaaaga aatgataagg acgagtatct
     3361 tttcagactt gccgacttta tccgttcgct caggaatgtt gaaagagttg aagttcttcc
     3421 gtatcacaca cttggtgtgt acaagtggaa agctctcggt tatgactatc ctctcgaggg
     3481 cattaatccc ccgacaaaag agcgtgttaa gaatgcggaa atgattctta acggtgaaaa
     3541 aacaagagat taatttctta tataacaacg gcagtaccga tttttttggt actgccgttt
     3601 ttgttttgat tttatttgtt attctgcttt tgtagttttt tcttcagcaa aattgatgaa
     3661 ttaaaggtg
//
