LOCUS       c00300_gnlPro..         3619 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KJHFNGNC_300.
ACCESSION   c00300_gnlPro..
VERSION     c00300_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 21:10:50
            Original ID  :: gnl|Prokka|KJHFNGNC_300
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3619
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1290
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg300_1"
                     /sec_met_domain="Gly_radical (E-value: 9.8e-43, bitscore:
                     132.9, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 3.1e-137, bitscore:
                     446.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="FSGDPVWATLEVAGMGQDGRSMVTKNDYRFLHTLEDMGPAPEPNL
                     TVLYSSRLPENFKKYAANISVTTSSVQYENDDVMRPVWGDDYSICCCVSATETGKEMQF
                     FGARANLAKCLLYAINGGVDEKTKQQVGPNYQPITSEYLDYDEVIAKYDKMMDWLAHLY
                     VGTLNMIHYMHDKYYYEAAEMALIDTKVERSFATGIAGFSHVVDSLSAIKYAKVKAIRD
                     EDGITTDFQVEGDFPRYGNDDDRADEIATTLLSTFLEKLKHIHTYRDSKPTTSILTITS
                     NVVYGKATGSLPDGRKAGEPLAPGANPSYGAEQNGLLASLNSVAKLDYEDALDGISNTQ
                     TINPDALGHSDDERSENLVHVLDGYFDQGAHHLNVNVFGKEKLIDAMEHPEKEEYANFT
                     IRVSGYAVKFIDLTREQQLDVIARTCHDRM"
     protocluster    1..3619
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2049]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2049
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3619
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3619
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             1309..2049
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 84; E-value:
                     3.9e-26)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg300_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.1e-29, bitscore:
                     90.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNKQIKGYVHSLESFGSVDGPGVRYVIFLSGCAMRCQFCHNPDT
                     WKMKQGELYTADELLKKALRYKGYWGSKGGITVSGGEPLLQMDFLTEFFKKAKAEGVHT
                     NLDTSGNPFTDQEPWHSGWLELMKYTDLVMLDIKQIDEQEHIKLTGHSNKNILAMAREL
                     SDMKKPVWIRHVLVPGGSDKDEYLHRLADFIHTLSNVERVEVLPYHTLGKFKWENLGLS
                     YPLEGVNPPTQERIDNARKILGAI"
     CDS             2512..3618
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AstE_AspA"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg300_3"
                     /sec_met_domain="AstE_AspA (E-value: 1.2e-10, bitscore:
                     28.6, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VKDLLSEIESYWTTRAEGYSEVNHKELNGMQKGAWLEVLKGQFPE
                     KAKDEIKILDIGTGPGFFPVILAEAGYKVTAVDYTQEMLDTAKRNAGNLCERISFYKMD
                     AQNLEFEDDVFDVVISRNLTWNLKDPKRAYEEWCRVLKPGGKLLNFDANWYGYLYDEEK
                     RLSYEEDRKSVESEHLDDHYLCTDIDRMEKIALQMPLSSINRPSWDRKFLKENGFESVA
                     VDTGIWQRVWSQEEKLNYHSTPMFMISAVKEEKNVWSENDGMGDSDGGYDRKRDLEDAM
                     LCAAPGMKKSGFLRLGGGEFSLPYTVICGSHPGKTVLITAAVHGGEYVGIQAAVELADK
                     LKPEKIHGRVILVKTVCRKEFEERSGSVC"
ORIGIN
        1 ttctccggtg acccggtatg ggcaactctg gaagtagcag gtatgggaca ggacggacgt
       61 tccatggtaa caaagaacga ctatcgtttc ctgcacacac tggaagatat gggaccggct
      121 ccggaaccaa accttacagt actttattct tcaagactgc ctgagaactt caagaaatat
      181 gctgcaaata tttctgttac aacaagttct gttcagtatg agaatgatga tgttatgcgt
      241 ccggtatggg gcgatgacta cagcatctgc tgctgtgtat ctgctacaga gacaggtaaa
      301 gagatgcagt tcttcggcgc tcgtgcaaac cttgcaaaat gccttcttta tgcgatcaac
      361 ggcggtgttg atgagaagac aaaacagcag gtaggaccaa attatcagcc gatcacttct
      421 gaatatcttg actatgatga agtaattgca aaatatgaca aaatgatgga ctggctggct
      481 catctgtatg tcggaacact gaacatgatc cactacatgc atgacaaata ctactacgaa
      541 gcagcagaga tggctctgat cgatacaaag gttgagcgtt ctttcgcaac aggtatcgca
      601 ggattctctc atgtagtaga ctccctgtcc gctatcaaat atgcaaaagt taaagctatc
      661 cgcgatgaag acggcattac aacagatttc caggttgagg gtgacttccc acgttatggt
      721 aatgatgatg acagagcaga cgagattgca acaactcttc tgtctacatt ccttgagaaa
      781 ctgaaacaca ttcatactta ccgtgattcc aagccaacca catctatcct tactattact
      841 tccaacgttg tttatggtaa ggctacagga tctcttccgg atggaagaaa agcaggtgaa
      901 cctcttgctc ctggcgcaaa cccatcttac ggtgcagagc agaacggtct tcttgcttcc
      961 ctgaactctg ttgccaagct tgactatgaa gatgctcttg acggtatctc caatactcag
     1021 acaatcaatc ctgatgctct gggacacagc gatgacgagc gttcagagaa tctggtacat
     1081 gtacttgacg gatactttga tcagggtgct catcacctca atgtaaacgt atttggtaaa
     1141 gagaaactga tcgacgctat ggaacatccg gaaaaagaag agtatgcaaa cttcacaatc
     1201 cgtgtatccg gatatgctgt taagttcatc gaccttacaa gagagcagca gttagacgtt
     1261 attgccagaa cctgccacga cagaatgtaa ttaatggatg atgatataat ggataataaa
     1321 cagataaaag gctatgttca ttctttagaa agcttcgggt ccgttgacgg acccggagta
     1381 agatatgtga tttttttaag cggctgtgcc atgagatgtc agttctgtca taatccggat
     1441 acctggaaga tgaagcaggg agagctttat acggcagatg agcttcttaa gaaagcttta
     1501 cgttataagg gctactgggg cagcaaaggc ggtatcactg taagcggtgg tgagccattg
     1561 cttcagatgg attttcttac agaatttttt aagaaggcaa aggcagaggg tgtgcatacg
     1621 aatcttgata ccagtggaaa tcccttcact gatcaggaac catggcattc cggatggctg
     1681 gagcttatga agtatacaga tcttgtaatg cttgatatca agcagattga cgagcaggag
     1741 catattaagc ttaccggtca ttccaataag aatattcttg cgatggcgcg ggaactttct
     1801 gatatgaaaa aacctgtatg gatccgtcat gtgcttgtgc cgggcggaag tgataaggat
     1861 gaatatctgc accggttagc tgattttatc catactcttt ccaatgtgga gagagtggag
     1921 gtgctccctt atcatacgct tggtaagttt aaatgggaga atctggggct ttcttatcca
     1981 ctggaggggg ttaatcctcc gacgcaggaa aggattgata atgcccggaa gattcttgga
     2041 gctatctgag taaaaatata tagctgtcat atcctctttt ggggggtatg gcagctttgt
     2101 ttttggttta aaaaggggac gcgaagcagg cgtatagttg taccggatac ggtttcggca
     2161 tctaagagat tctaagccct cgaaaatccg ctaaaagcat gagacgaaaa gtccggaatt
     2221 actcgctgtc gctcagacaa gggacttttc gtctcataac gcagatttcc gaggactaag
     2281 aatctctaag atgcctgcaa atgcatccgg tacaactata cgcctgcttc gctggttttg
     2341 gttaagatat aatacaaggt ggagtgtgaa ttataagaag gactttgttt atataaaaat
     2401 gaattgagac gtgaaatata tcccccggga gggcttgttg gaaaggggag ggatttgata
     2461 taattttggc tatatatgat tataaattgc gtgcatggag gttggcataa ggtgaaggat
     2521 ttattatcgg agattgagag ttactggact acccgggctg aggggtattc tgaagttaat
     2581 cataaggaat tgaatgggat gcagaagggg gcatggcttg aggtgttgaa agggcagttc
     2641 ccggagaaag cgaaggatga gattaagatt ttggatatcg gaacaggacc tggatttttt
     2701 cctgttattc tggctgaggc gggatataag gtgactgctg ttgattatac acaggagatg
     2761 ttggatactg cgaagaggaa tgccggtaat ctttgtgagc ggatttcttt ttataagatg
     2821 gatgctcaga acctggaatt tgaggatgat gtgtttgatg ttgtgatcag caggaatctg
     2881 acatggaatc tgaaggatcc taagagagct tatgaagagt ggtgtcgtgt actgaaaccg
     2941 ggcggaaagc ttcttaattt cgatgcaaac tggtacggtt atctttatga tgaggagaaa
     3001 cgcctgtctt atgaggaaga ccgaaagagt gtggagagtg agcatctgga tgatcattac
     3061 ctctgtacgg atattgacag aatggagaag attgcactgc agatgcctct ttcttctatt
     3121 aacagaccgt cctgggacag gaagtttctg aaggagaacg ggtttgaatc tgttgcagtg
     3181 gataccggga tctggcagcg ggtgtggagt caggaagaga agctgaatta tcattctacg
     3241 ccgatgttta tgatcagcgc tgtgaaggaa gagaaaaacg tctggtccga gaatgatgga
     3301 atgggtgatt ctgatggcgg atatgacagg aaaagagatt tggaggatgc catgctgtgt
     3361 gctgctccgg gtatgaaaaa gagtggattt ctgagactgg gcggaggtga gttttcgctt
     3421 ccttatacag taatctgcgg ttctcatccc ggaaagacag ttctgatcac cgctgcggtc
     3481 cacggaggag aatatgtggg aatccaggct gctgttgagc ttgcggataa gctgaaaccg
     3541 gagaagatcc atggaagagt gattttggtc aagactgtgt gcaggaagga atttgaggag
     3601 aggagcggca gtgtctgcc
//
