LOCUS       c00145_gnlPro..         4012 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MBPGOLJE_145.
ACCESSION   c00145_gnlPro..
VERSION     c00145_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 21:06:53
            Original ID  :: gnl|Prokka|MBPGOLJE_145
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4012
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4012
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[162:3155]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      163..3155
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4012
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4012
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(163..918)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 76.2;
                     E-value: 1e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg145_1"
                     /sec_met_domain="Radical_SAM (E-value: 2.2e-31, bitscore:
                     97.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDNAVKGFVHSIETFGSVDGPGVRFVIFLSGCAMRCQFCHNPDT
                     WKHDENTVEYTADELLNKALAYRPYWKNKGGITVSGGEPLLQIDFLLDLFKKAKKQGVN
                     TCIDTSANPFTTKEPFYSKWKELMEYTDLLLLDIKHIDNEQHKILTGQPNTNILEMARQ
                     LSDMGKPVWIRHVLVPERNDKDEYLFRLADFIRSLKNVERVEVLPYHTLGVYKWKALGY
                     DYPLEGINPPTKERVKNAEMILNGEKTRD"
     CDS             complement(918..3155)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg145_2"
                     /sec_met_domain="Gly_radical (E-value: 3.1e-42, bitscore:
                     131.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 9e-273, bitscore:
                     893.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREEWTNFAGGKWESDIDVRDFIQRNYTPYDGDQSFLEGPTEATD
                     KLWGELKELQKEERAKGGVLDMETEVVSGITAYGAAYLDKDLEKVVGLQTDKPLKRAFM
                     PYGGIKMAEQSCQMYGYTPSPKLHEIFTKYHKTHNQGVFDIYTPEMKLARHNKILTGLP
                     DTYGRGRIVGDYRRVALYGIDRLIEGKKEDFAETNRQGMRRGDFQLREEIADQIRALND
                     MKEMALSYGFDISQPAKNAAEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIYIERDL
                     ENGTLTESQAQELVDHFVMKCRMVKFARIESYNQLFSGDPVWATLEVAGLGIDGRSMVT
                     KNDYRFLHTLENMGPSPEPNLTVLYSSRLPENFKKYAAHISVTTSSIQYENDDVMRPVW
                     GDDYSICCCVSATQTGKEIQFFGARANLAKCLLYAINGGIDEKTKMQVGPEYTPITSEY
                     LDYDEVIKKYDTMMDWLAHLYVGTLNMIHYMHDKYYYEAAEMALIDTDVRRTFATGIAG
                     FSHVVDSLSAIKYAKVKVIRDEDGIAVDFETEGDFPRYGNNDERADKIAVDLLKTFMAK
                     LKYCHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQNGLLA
                     SLNSVAKLPYEYALDGISNTQTINPGALGHNDDERVDNLVNVMDGYFDQGAHHLNVNVF
                     GTEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVIARTCHDRM"
     CDS             complement(3422..3994)
                     /locus_tag="ctg145_3"
                     /transl_table=11
                     /translation="MLPIQNCTNKKTVYTNRVRRLAVRSDNMKTDRVITAMIEYFGSDK
                     KRIHHFLKVYSFAKTIGESENLLPDDQELLEISAIVHDIGIKVSEEKYNSSAGKYQELE
                     GPHEAEKLLAALGYEKTFIDKVCYLVGHHHTYGNIDTLPYRILVEADFLVNLYEDDSSR
                     SAAEQAYDKIFRTNTGKNLLKIMFLIP"
ORIGIN
        1 tacgctgaga tttctcaaca aggacagctt aaaaatcttt gaatacacct ttgattcatc
       61 aattttgctg aagaaaaaac aacaaaagca gaataacaaa taaaaatcaa aacaaaaacg
      121 gcagtaccaa aaaaatcggt actgccgttg ttatataaga aattaatctc ttgttttttc
      181 gccgttaaga atcatttccg cattcttaac acgctctttt gtcgggggat taatgccctc
      241 gagaggatag tcataaccga gagccttcca cttgtacaca ccgagggtgt gatacggaag
      301 cacctctaca cgctcaacat tcttgagcga acggataaag tcggcaagtc tgaaaagata
      361 ctcgtcctta tcatttcttt caggcacaag aacatgtctt atccatacgg gcttgcccat
      421 atcggaaagc tgtcttgcca tttcaagaat attggtgttt ggctgtcctg taaggatttt
      481 gtgctgttcg ttgtcgatat gctttatatc aagcaacagc aaatccgtat attccattag
      541 ctctttccac tttgaataga acggctcttt ggttgtaaac gggttggcgc ttgtgtcaat
      601 acaggtatta acgccctgct ttttagcctt cttgaacaaa tcaagcaaaa agtcaatctg
      661 taaaagcggc tcacctccgc ttactgtgat accgccctta tttttccaat aggggcggta
      721 tgcaagcgct ttattaagca actcgtcggc ggtgtattcc acggtgtttt catcgtgttt
      781 ccaggtgtcg ggattgtggc agaactggca acgcattgcg caaccgctca gaaaaatcac
      841 aaatctcaca cccggaccgt caacagagcc aaaagtttct attgagtgta caaagccttt
      901 aacagcgttg tccgacatta cattctgtcg tggcaggttc tggcaataac atccagctgc
      961 tgctctcttg taaggtcgat gaacttaaca gcataacccg atacacggat tgtgaagtta
     1021 gcgtactctt ccttctcagg atgctccata gcgtcaatga gtttttctgt accgaataca
     1081 tttacattga ggtggtgtgc gccctgatca aagtagccgt ccataacatt tacgaggttg
     1141 tcaacacgct cgtcatcgtt gtgtccgaga gcacccgggt tgattgtctg agtatttgaa
     1201 ataccgtcaa gagcgtactc gtaaggaagc ttagcaacag agttaagtga agcaagaaga
     1261 ccgttctgct ctgcaccgta gcttgggtta gcacccggtg aaagcggctc gcctgctttt
     1321 ctgccgtcag gaagtgaacc tgtagcctta ccgtaaacaa catttgatgt gattgtaagg
     1381 atagaagttg taggctctga atctctgtat gtgtggcaat acttgagctt agccatgaat
     1441 gtcttgagaa gatcaacggc aatcttatct gctctctcgt cattgttacc gtatcttggg
     1501 aagtcgccct ctgtttcaaa gtcaacagca atgccgtcct cgtcacggat aaccttaacc
     1561 ttagcgtact taatagcgct gagtgagtca acaacatgtg agaagcctgc aatacctgtt
     1621 gcaaatgttc ttcttacatc tgtatcgata agagccattt ctgctgcctc atagtagtac
     1681 ttatcatgca tgtagtgaat catgttaagt gtgcctacat agaggtgagc aagccaatcc
     1741 atcattgtgt catacttctt gataacttcg tcataatcaa ggtactctga tgtgataggt
     1801 gtatattcag gaccaacctg catctttgtc ttttcatcaa taccgccgtt gatagcgtag
     1861 agaaggcact tagcaaggtt agctcttgcg ccgaagaact ggatttcctt acctgtctgt
     1921 gttgcggata cgcagcagca gattgagtaa tcatcgcccc atacaggacg cattacatca
     1981 tcgttctcgt actgaattga gcttgttgta actgaaatat gagcagcata cttcttgaag
     2041 ttctccggaa gtcttgaaga ataaagaact gtgaggtttg gctcaggtga aggtcccata
     2101 ttctcaagtg tgtggaggaa acggtagtcg ttctttgtaa ccattgaacg gccgtcaatg
     2161 ccaagacctg caacttcgag tgtagcccat acaggatcgc ccgagaagag ctggttgtaa
     2221 gactcaattc ttgcaaactt aaccatacgg cacttcataa caaagtggtc aacgagttcc
     2281 tgagcctgtg attctgtaag tgtaccgttc tcaaggtcac gctcgatgta gatgtcgagg
     2341 aatgttgata cacgaccaac gctcattgca gcgccgttct gagtcttgat agcagcaaga
     2401 taaccgaagt agagccactg aactgcctct gcagcgttct tagcaggctg tgaaatatca
     2461 aagccgtaag aaagtgccat ttccttcata tcgttaagag cacgaatctg atcggcgatt
     2521 tcttcacgaa gctggaagtc gcctcttctc ataccctgac ggtttgtttc tgcaaagtcc
     2581 tctttcttgc cttcgataag acggtcaata ccgtaaagag caactcttct gtagtcaccg
     2641 acgattctgc ctctgccgta tgtgtcggga agacctgtaa gaatcttgtt gtgacgggca
     2701 agcttcatct caggagtgta gatatcgaat acaccctggt tgtgagtctt gtgatatttt
     2761 gtaaagattt cgtggagttt agggcttgga gtgtagccat acatctggca tgactgctca
     2821 gccattttga taccgccgta aggcataaat gcacgcttga gaggcttgtc tgtctgaaga
     2881 cctacaacct tttcaaggtc tttatcaaga tatgcagcgc cgtaagctgt gatgccgctt
     2941 acaacctcag tttccatatc aagaacgccg cccttggctc tttcttcctt ctggagttcc
     3001 ttgagctcgc cccagagctt gtctgtagct tctgtaggac cttcaaggaa agactggtcg
     3061 ccgtcataag gtgtgtagtt tctctgaatg aagtctctga catcaatgtc ggactcccat
     3121 ttgccacctg caaaatttgt ccattcttct ctcattactg taaaacctcc gataatgata
     3181 attaatactg aattttgtat atgacacaaa aaaatgccga ctcaattcca agcagacgcc
     3241 cggtaaggtc agctccttga aaaaatcgtc atacatcctt gtattctgtc atttattcac
     3301 ttttataatc atataaccaa caccattaaa ataacacttt ttaagggcgt tgtcaagcat
     3361 tttgagatac tttttacacc actttttcac actcaaagca ctatggtaaa tgagtacaat
     3421 tttagggtat gaggaacatt attttcagca gattttttcc tgtatttgtg cggaatattt
     3481 tgtcatatgc ctgctctgct gcactgcggc ttgaatcgtc ctcataaagg ttcacaagga
     3541 agtctgcctc taccaaaatt cggtacggca aagtatcaat atttccatag gtgtgatggt
     3601 gtcccacaag gtaacaaact ttgtcaataa atgttttttc gtagccgaga gcggcaagca
     3661 acttttcagc ctcgtgagga ccttcaagtt cctgatactt tcctgcagac gaattgtact
     3721 tttcttcgct cacctttatg ccgatgtcgt gaacgattgc ggagatttca agaagctcct
     3781 gatcgtcggg caaaagattt tcactttcac cgattgtctt tgcgaatgaa taaaccttga
     3841 gaaaatggtg aattcttttc ttgtcacttc caaaatattc aatcatagca gttataaccc
     3901 tgtcggtttt catattatca ctccttacgg cgagccgccg taccctattc gtgtagacag
     3961 tttttttgtt tgtgcaattc tgtatgggta gcattgcgac tatatttcag ac
//
