LOCUS       gnlProkkaEPBLLOKO_227   3079 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EPBLLOKO_227.
ACCESSION   gnlProkkaEPBLLOKO_227
VERSION     gnlProkkaEPBLLOKO_227
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-01 15:16:37
            Original ID  :: gnl|Prokka|EPBLLOKO_227
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3079
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3079
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[197:3077]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      198..3077
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..3079
                     /SMILES="NC(CS)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..3079
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /tool="antismash"
     CDS             complement(2..73)
                     /locus_tag="ctg227_1"
                     /transl_table=11
                     /translation="MRKLKSLVCGSKFGQFYIDAISKM"
     CDS             complement(198..3077)
                     /NRPS_PKS="Domain: AMP-binding (2-394). E-value: 7.3e-83.
                     Score: 270.2. Matches aSDomain:
                     nrpspksdomains_ctg227_2_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (874-941). E-value: 4.8e-20. Score:
                     63.4. Matches aSDomain: nrpspksdomains_ctg227_2_PCP.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg227_2"
                     /sec_met_domain="AMP-binding (E-value: 5.9e-98, bitscore:
                     315.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.2e-17, bitscore:
                     49.4, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEVCPEDIALIDAGSGRSVSYGELKTRATAVAAGISEKDIKGVP
                     IALTLPRGIEQIEAALGILLSGNSYLPVSLSQPKDRRALIHEKTGVRYVVTNRELSEKL
                     DWPEGTEILVMEGMEEGQENVRLPEVSPKDSAYIIMTSGSTGVPKGVEIAHESAWNTVQ
                     DINEKYHVTSADRALAVSAMDFDLSVYDVFGILGAGGTLVLLPEQERRNADYWLEQVLK
                     YQITVWNSVPVLLDMLLIRAESMKQKLPIRAVMLSGDWIGMDLPQRVAAWTEDCQFVAM
                     GGATEASIWSNYQNVTLPMPKNWKSIPYGKPLRYQAYRVVDEYGRDCPYWAEGELWIGG
                     FGVAKGYRGDSALTGQKFITDQYGRWYRTGDLGRIWDDETIEFLGRKDHQVKIRGHRIE
                     LGEIEHAIQEFPGVAHAVVDTVSDGHGNKTLAAYIGAPLQEDSKVTTYLYGTDIFGGGW
                     KELKDDVSNWQMQQERKTAYKNFLAYADQRCVQLMLETLIELGVFVSEKEVLSQKEIFE
                     KGSITETQKNTVARWLEILKKEGILREEDGRLSRTGKEVAVPEKAGDAETYFKKLKPYL
                     KHMVTGNEVPLDVFYQKDSALAPNMLLRRIPGYEETVERLVQELKLLIEGRRKEPLQII
                     EIGTRDAAITRQILNVLEDVSVAYTYVDSSKYFLQEAEKELAGYERVEFEMLNLEESMD
                     KQQMALHSYDVVISVNALHRNIDAVDAVKKVAELLKPNGILLMTDLVVRTYLQELTATF
                     LENGFADIRDKRKEAGMVTPDCLLWRECLSEAGLGQDIVVTEEYGRCICCSRQQASVLS
                     YYDGALREYLSEKLPEYMVPQNYHFMAQLPTLSNGKINRKKLREDFKEETSVIRFSKAV
                     TETEEKLLDIWKRMFGYENLGTEDNYFVLGGDSLIATKLISDIQKEFGCKIMISDIFEN
                     VTIKLLAQKIDLVRQNVIMEDIEMECGEI"
     aSDomain        complement(255..455)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg227_2_PCP.1"
                     /evalue="4.80E-20"
                     /label="ctg227_2_PCP.1"
                     /locus_tag="ctg227_2"
                     /protein_end="941"
                     /protein_start="874"
                     /score="63.4"
                     /tool="antismash"
                     /translation="EEKLLDIWKRMFGYENLGTEDNYFVLGGDSLIATKLISDIQKEFG
                     CKIMISDIFENVTIKLLAQKID"
     aSModule        complement(255..3071)
                     /complete
                     /domains="nrpspksdomains_ctg227_2_AMP-binding.1"
                     /domains="nrpspksdomains_ctg227_2_PCP.1"
                     /locus_tags="ctg227_2"
                     /monomer_pairings="Cys -> Cys"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(1866..1931)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg227_2_0003"
                     /evalue="2.50E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg227_2"
                     /protein_end="404"
                     /protein_start="382"
                     /score="41.8"
                     /tool="antismash"
                     /translation="FLGRKDHQVKIRGHRIELGEIE"
     aSDomain        complement(1896..3071)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg227_2_AMP-binding.1"
                     /evalue="7.30E-83"
                     /label="ctg227_2_AMP-binding.1"
                     /locus_tag="ctg227_2"
                     /protein_end="394"
                     /protein_start="2"
                     /score="270.2"
                     /specificity="substrate consensus: Cys"
                     /tool="antismash"
                     /translation="EVCPEDIALIDAGSGRSVSYGELKTRATAVAAGISEKDIKGVPIA
                     LTLPRGIEQIEAALGILLSGNSYLPVSLSQPKDRRALIHEKTGVRYVVTNRELSEKLDW
                     PEGTEILVMEGMEEGQENVRLPEVSPKDSAYIIMTSGSTGVPKGVEIAHESAWNTVQDI
                     NEKYHVTSADRALAVSAMDFDLSVYDVFGILGAGGTLVLLPEQERRNADYWLEQVLKYQ
                     ITVWNSVPVLLDMLLIRAESMKQKLPIRAVMLSGDWIGMDLPQRVAAWTEDCQFVAMGG
                     ATEASIWSNYQNVTLPMPKNWKSIPYGKPLRYQAYRVVDEYGRDCPYWAEGELWIGGFG
                     VAKGYRGDSALTGQKFITDQYGRWYRTGDLGRIWDDETIEFLGRKDHQVKIR"
     CDS_motif       complement(2004..2093)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg227_2_0002"
                     /evalue="1.30E-10"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg227_2"
                     /protein_end="358"
                     /protein_start="328"
                     /score="33.6"
                     /tool="antismash"
                     /translation="PYWAEGELWIGGFGVAKGYRGDSALTGQKF"
     CDS_motif       complement(2613..2672)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg227_2_0001"
                     /evalue="2.80E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg227_2"
                     /protein_end="155"
                     /protein_start="135"
                     /score="26.0"
                     /tool="antismash"
                     /translation="AYIIMTSGSTGVPKGVEIAH"
ORIGIN
        1 tcatcttact aatagcatct atataaaatt gtccaaactt actaccacat acaagtgact
       61 ttaattttct cattttctca ccttctcaat aaaataggga caagttacac tcctcaatat
      121 ctaatactcg taatttgccc ctaaaaatgc gagctatttt tcattatgaa atatactaaa
      181 aaacagattt gacaatgtta aatttctcca cactccatct cgatatcttc cataatgaca
      241 ttttgtctta ctaaatctat cttttgagcc aataatttta tcgtaacatt ttcaaaaata
      301 tcactaatca taattttaca gccaaattct ttctgaatat ctgaaatcaa tttggttgct
      361 attaatgaat caccacctaa aacaaaataa ttatcttcag tccctaaatt ttcataacca
      421 aacattcgtt tccaaatatc caaaagtttt tcttctgtct ccgttactgc ttttgaaaat
      481 ctgataaccg aagtttcttc tttaaaatct tcccgtaatt ttttccggtt aatctttcca
      541 ttggacaatg ttggaagctg tgccataaaa tgatagtttt ggggaaccat atactccggc
      601 agcttttccg acagatattc cctaagtgct ccatcataat aggacaagac agatgcctgc
      661 tgcctactgc agcatataca tctcccatat tcttctgtta caacaatgtc ctggcctaat
      721 cctgcctctg ataagcattc tctccacagc agacaatctg gggtaaccat ccccgcttct
      781 tttctcttgt cccgaatatc tgcgaaacca ttttccaaaa aagttgctgt aagctcctga
      841 agatatgtcc ttaccacaag atctgtcatt aacaatatcc catttggctt cagtaattcc
      901 gctactttct ttaccgcatc cacagcgtca atatttctat gcaaagcatt taccgatatc
      961 acgacgtcat aactatgcag agccatctgc tgcttgtcca tgctttcttc cagattcagc
     1021 atttcaaact cgactctttc atatcccgca agctctttct ccgcttcctg cagaaaatat
     1081 tttgaagaat ccacatatgt atacgccaca cttacatctt ccagcacatt taaaatctgt
     1141 cttgtaattg cagcatccct tgtcccaatc tctataatct ggagtggttc tttcctgcgc
     1201 ccttctatca gaagttttaa ttcctgcacc agtctttcca cggtctcctc gtaacccgga
     1261 atcctccgaa gcaacatatt cggagccagc gccgaatctt tctggtaaaa tacatccagc
     1321 ggaacttcat tcccagtaac catatgcttc agatacggct tcagtttctt aaaatacgtt
     1381 tccgcatctc cagctttttc cggtacagct acttccttcc cggtacgcga tagtctgccg
     1441 tcctcttctc tcaggattcc ttccttcttt aaaatttcca accatcttgc cacagtattc
     1501 ttctgtgttt ccgtaatgct ccctttttca aaaatttcct tctgcgaaag aacttccttc
     1561 tctgatacaa aaacgcctaa ctctatgagt gtctccagca taagctgtac acatctctgg
     1621 tctgcatagg caaggaaatt tttgtatgct gtcttccgtt cctgctgcat ctgccaattg
     1681 ctgacatcat ccttcagttc tttccagccc ccgccaaaga tatctgtgcc gtacaaatac
     1741 gttgtaactt tagaatcttc ctgcagcggc gctccgatat aagcagccag cgttttattg
     1801 ccatgaccat cactaactgt atcaacaact gcatgagcca cacccggaaa ctcctgaata
     1861 gcatgttcaa tttctcccaa ttcaatcctg tgtccacgga tctttacctg atggtctttt
     1921 cttcccaaaa attcaattgt ctcatcatcc cagattcttc cgagatcgcc ggtccgatac
     1981 catcttccat actgatctgt gataaatttc tgccccgtaa gcgcagaatc tcccctatat
     2041 ccttttgcta caccaaaacc gccaatccag agttcgcctt ctgcccagta agggcaatcc
     2101 cttccatact catccacaac acgatatgcc tgatatctga gtggctttcc ataaggaata
     2161 gatttccagt ttttcggcat cggcagggta acattctgat aattggacca gatacttgcc
     2221 tctgtagctc ctcccatagc gacaaactgg caatcctctg tccatgccgc aaccctctgc
     2281 gggagatcca tcccaatcca atctccggaa agcataaccg ccctgatcgg cagtttctgt
     2341 ttcatggatt ccgcccgtat caaaagcata tccagaagca ccggcactga attccaaacg
     2401 gtaatctgat atttcaacac ttgttccagc cagtaatccg cattccttct ctcctgctct
     2461 ggaagcaata ccaatgttcc tcctgcgcct aaaattccaa aaacatcata taccgacagg
     2521 tcaaaatcca tagctgatac agccagcgcc cgatctgcac ttgtcacatg gtacttttca
     2581 ttaatatcct gaaccgtatt ccatgcgctt tcatgcgcaa tctctacacc cttcggaaca
     2641 cctgtagagc ctgacgtcat gatgatatag gcgctgtcct tcggcgacac ctctggcagt
     2701 ctcacatttt cctgtccctc ttccatacct tccatcacaa ggatttccgt accttccggc
     2761 cagtccagtt tttcggataa ttcccgattc gtgaccacat agcggactcc cgtcttctca
     2821 tggattaacg ctctccgatc tttaggctgg cttaagctga ccggaaggta tgaatttccg
     2881 gaaagcaaaa ttcctaatgc cgcctcgatc tgttctatcc ctcgcggcag ggtaagtgca
     2941 atcgggacgc ccttaatatc cttttctgaa attcctgccg ccactgctgt tgcccgtgtt
     3001 tttaattccc cataggatac cgaccttccg cttcccgcat caataagcgc aatatcttcc
     3061 gggcaaactt ccgcatggt
//
