LOCUS       c00089_gnlPro..         6909 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EPBLLOKO_89.
ACCESSION   c00089_gnlPro..
VERSION     c00089_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 00:35:51
            Original ID  :: gnl|Prokka|EPBLLOKO_89
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6909
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6909
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1887:5136]"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1888..5136
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /neighbourhood="10000"
                     /product="Putrescine2spermidine"
                     /protocluster_number="1"
     cand_cluster    1..6909
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /kind="single"
                     /product="Putrescine2spermidine"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6909
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Putrescine2spermidine"
                     /region_number="1"
                     /rules="(minimum(2, [AdoMet_dc, Spermine_synt_N,
                     Spermine_synth]) or (Orn_DAP_Arg_deC and Sacchrp_dh_C))"
                     /tool="gutsmash"
     CDS             428..1885
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) OKR_DC_1_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) OKR_DC_1_7_arginine"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg89_1"
                     /sec_met_domain="OKR_DC_1_C (E-value: 1.5e-14, bitscore:
                     43.2, seeds: 113, tool: rule-based-clusters)"
                     /sec_met_domain="OKR_DC_1_7_arginine (E-value: 3.3e-127,
                     bitscore: 413.0, seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDYERQQHAPIYEALERFRKKRVVPFDVPGHKRGRGNPELAQLLG
                     EKCVGLDVNSMKPLDNLCHPVSVIREAEELAADAFGAAHAFLMVGGTTSAVQSMILSVC
                     KAGDKIILPRNVHKSAINALVLCGAIPVYVNPEVNAQLGISLGMEVSQVEKAMDENPDA
                     VAVLVNNPTYYGICSDLRTIVKKAHARGMKVLADEAHGTHLYFGRNLPVSGMAAGADMA
                     AVSMHKSGGSLTQSSLLLLNKSMNAEKVRQIINLTQTTSASYLLLSSLDISRRNLALRG
                     EESFEKVAGMAQYAREEINEIGGYYAYGMDLINGTSVFDFDVTKLSIYTLGNGLAGIEV
                     YDLLRDEYDIQIEFGDICNILAYISIGDRLQDIERLVGALADIERLYKKDSTGMLSGEY
                     IAPAVVASPQQAFYAEKESLPMEQAAGRISGEFVMCYPPGIPILAPGEMVTQEIVEYIL
                     YARDKGCSMQGMEDPKVEYLQVLKGGI"
     CDS             1888..2748
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Spermine_synth"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Spermine_synt_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg89_2"
                     /sec_met_domain="Spermine_synth (E-value: 1.9e-54,
                     bitscore: 172.6, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Spermine_synt_N (E-value: 2.8e-16,
                     bitscore: 48.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELWYSEFHTGNVKLSVRINRQLFSGESEFQRIDVFESEEFGRFV
                     ALDGEIVFSDKDEFIYDEMVTHVPMTVHPNVKNVLIIGGGDGGVARELIHYPQIESIDV
                     VESDKMFVDVCAEMFPDIAQGLKDERVNIYYEDGLRFLRNKKARYDLIINDSTDPLGHT
                     EGLFTKEFYGSCYKALRDDGIMVYQHGSPFYDEDEVECRKMHRKVFRSFPVSRVYQAHI
                     PTCPSGYWLFGFASKKYHPLEDFRPERFDNLNIETWYYTTNLHRGAFMLPKYVEDLLEE
                     EENSL"
     CDS             2745..4025
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Sacchrp_dh_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Sacchrp_dh_NADP"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg89_3"
                     /sec_met_domain="Sacchrp_dh_C (E-value: 2.3e-44, bitscore:
                     141.1, seeds: 375, tool: rule-based-clusters)"
                     /sec_met_domain="Sacchrp_dh_NADP (E-value: 7.7e-37,
                     bitscore: 115.2, seeds: 81, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRLLVIGCGGVASVAIRKCCQNSDVFTEIMIASRTKEKCDALKE
                     KIESTTKTKIETAKVDADNAAEVAELIRAYKPDAVLNVALPYQDLTIMDACLEAGADYI
                     DTANYEAEDTEDPTWRAIYEKRCKEKGFTAYFDYSWQWAYNEKFKEAGLTALLGTGFDP
                     GVTSVFSAYALKHYFDEIHTIDILDCNGGDHGYPFATNFNPEINLREVSANGSYWEDGH
                     WVETEPMEFKSVYDFPEVGKKDMYLLHHEEIESLAKNIPGVQRIRFFMTFGQSYLTHMK
                     CLENVGMLSTAPVEFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGVKDGKEK
                     SIYIYNVCDHQECYKEVESQAISYTTGVPAMIGSMMVVTGQWKKPGVFNVEEFDPDPYM
                     EALNKWGLPWKVCEDPELVKVWKANED"
     CDS             4015..5136
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Putrescine2spermidine: Orn_DAP_Arg_deC"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg89_4"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 1.2e-15,
                     bitscore: 46.4, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIDQLQTPCYVIDEKKMRENLEILKKVQEDTGCKILLAQKAFSG
                     FALYPMIGKYLAGTTASGLFEARLGYEEMGKENHVFSPAYRTEDIEELDRICDHIIFNS
                     TAQLKKFKEVCTGASLGLRINPECSTQGDHAIYDPCAPGSRLGVTRENFDGECLKWIDG
                     LHFHTLCEQNADDLEKTLQAVEEKFGEFLSEVSWLNMGGGHHITREDYDVKLLEKCIQH
                     MKETYDLDIYLEPGEAVALNAGYLVTEVMDIVENEIRTLILDASAACHMPDVLEMPYRP
                     PLKDSGEAGEKAYTYRLSSCTCLAGDVIGDYSFDREIRIGDKLYFMDMAIYSMVKNNTF
                     NGMPLPDIAVMHEDGECEVIRHFGYEDFKSRLS"
     CDS             5084..6295
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PAD_porph"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg89_5"
                     /sec_met_domain="PAD_porph (E-value: 2.3e-128, bitscore:
                     416.5, seeds: 436, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VRLSDISDTKTLRAGFPDICRAIKEKETDMEVTEKNRTKYRMPGE
                     FEPHEGCVMIWPERPGSWNYGAREAQKAFVKVAEAIGVSEKVYMLVSKAQMENAKNQLG
                     NVSGVTLLECETDDAWARDVGATMVLDEKGAVCGVDWQFNAWGGTFDGLYRNWEKDDRV
                     AAFICRTLGCPCLDARPFVLEGGSIHSDGEGTLIVTEACLLSQGRNPQMSREQIEEQLK
                     YWLGVHKIIWLPCGIYQDETNEHVDNVCAFVRPGEVVLAWTEDETDPQYAMSLADLKVL
                     EQETDAKGRKFKIHKLPIPQKPVCLTQEDVDGFVFEEGEDEREAGERMAASYVNFYISN
                     GGIILPQFGDENDKRAVEILQKCFPERRIYPIDARAIIVGGGNIHCITQQIPGKISDLD
                     AEKL"
     CDS             6376..6909
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) CN_hydrolase"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg89_6"
                     /sec_met_domain="CN_hydrolase (E-value: 7.9e-44, bitscore:
                     138.6, seeds: 52, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRNVTVAAVQMKCSKSVEKNIAHAEELVRQAAAKGAEIVLLPELF
                     ERPYFCQERRYEYYEYAQTAEENPAVRHFSRVAAELGIVIPVSFYEKEVNNTYNSVAVL
                     DADGKNLGIYRKTHIPDDHYYQEKFYFTPGDTGFKVFDTRFGTIGVGICWDQWFPETAR
                     CMALQGAELLFYPTA"
ORIGIN
        1 taataatcag tgcgtaagca cacatcatct tgtgaaacgg tcaataagag catagcatat
       61 atttgccagc gattgaagac gacagcagtc atgaatattt cagaatacgg gaacaggcac
      121 aggatacaga cggatttata cgggaaatcc ggtcagtata taaagagaca gagcctgaga
      181 aattctatag atcatgagag aagcagatgg tgaatgaact tatctgcttc tttttttgtg
      241 cagatatagc aaatataaag gatatggttg gagataaacg ctttgcgagg atgtgtctca
      301 catgcgttcg acttgaatat atagagaaat aattgaatca ttgtgccggt ttgaggcagt
      361 atacaattgc tgaatgatag aactgtattt gccggtacat gacgacagga aaagaggaac
      421 atgtcaaatg gattatgaaa gacaacagca tgcgcccata tatgaagcac tggaacgttt
      481 ccgaaagaag agagtcgttc cctttgatgt gccgggacat aaaagaggaa gaggaaatcc
      541 ggagcttgca cagttgctgg gagagaaatg tgtggggctg gatgttaatt ctatgaagcc
      601 gctggacaat ctctgccacc cggtttcggt gatcagagaa gcggaggagc ttgcagcaga
      661 tgcttttggc gcagcgcatg cattccttat ggtaggagga accacatctg cagtgcagag
      721 catgatcctg tctgtctgca aggcagggga taagatcatt ctgccgcgaa atgtacataa
      781 aagtgccatc aatgccctgg tactgtgcgg tgcaattccg gtttatgtaa acccggaggt
      841 gaatgcgcag ctgggcattt cccttggcat ggaagtcagt caggtggaga aggctatgga
      901 tgagaatccg gatgcggtgg cagttcttgt aaataaccct acctattatg gaatctgttc
      961 ggatctgcgg actatcgtaa agaaggctca tgcaagagga atgaaggtgc tggcagatga
     1021 ggctcatgga acccatcttt atttcggcag aaaccttcct gtatccggaa tggcggcagg
     1081 tgctgacatg gcggcagttt ccatgcacaa atccggcgga agtctgacac agagttcgct
     1141 gctgcttttg aataagagta tgaatgcaga gaaagtgcgc cagatcatca atctgactca
     1201 gaccacaagc gcttcttatc ttctgttgtc cagtctggat atctccagaa gaaatctggc
     1261 actcagggga gaggaatcct ttgaaaaggt tgcagggatg gcgcagtatg ccagagagga
     1321 gatcaatgag atcggcggtt attatgcata tggtatggat ctgatcaatg gaaccagtgt
     1381 ttttgatttt gatgttacaa aactctccat ttatacactg ggaaatgggt tggcaggtat
     1441 tgaagtttat gatcttctca gagatgaata tgatattcag atcgagtttg gagatatctg
     1501 taatattctg gcttacattt ccatcggaga caggcttcag gatattgaga gactggtagg
     1561 ggcattggct gatattgaac gtctgtataa gaaggacagc acaggtatgc tttccgggga
     1621 atatatcgca cctgcagtgg tggcgtctcc acagcaggca ttttatgcgg agaaagaatc
     1681 tctgcccatg gaacaggcgg caggcaggat cagcggtgaa tttgttatgt gttatcctcc
     1741 gggaatcccg attcttgctc ctggggaaat ggtgacacag gaaattgtag aatacattct
     1801 ttatgcaaga gacaaaggct gttccatgca gggaatggag gatccgaagg tagaatatct
     1861 tcaggttctg aaagggggta tctgatcatg gaattatggt attccgagtt ccatacagga
     1921 aatgtaaagc tttctgtccg gattaaccga cagttatttt caggggaaag tgaatttcag
     1981 agaatcgatg tgtttgagtc tgaagaattt ggaagatttg tggctctgga cggtgagatc
     2041 gtattctctg ataaagatga atttatttat gatgaaatgg tcacgcatgt gccaatgaca
     2101 gtacatccca atgtaaaaaa tgttctgatc atcggcggcg gtgacggtgg agttgccaga
     2161 gaactgatcc attatccaca gattgagagc attgatgtgg tggaatccga taaaatgttt
     2221 gtggatgtat gtgcggagat gtttccggat attgcccagg gactgaagga tgagagagtc
     2281 aacatttatt atgaagacgg ccttcggttc ctgcggaata aaaaggccag atatgacctg
     2341 atcatcaatg attccacaga tccgctggga catacagagg gactttttac aaaggaattc
     2401 tacggaagct gttataaggc tctcagagat gacggaatca tggtttatca gcatggaagt
     2461 cctttttatg atgaggatga ggtggaatgc agaaagatgc acagaaaggt tttccggtca
     2521 tttccggtga gcagggttta tcaggcacat atccctacct gtccctcggg atactggctt
     2581 tttggatttg catcaaagaa atatcatcca ctggaagatt tcaggccgga gaggtttgat
     2641 aatcttaata tagaaacatg gtattataca acaaaccttc acagaggagc atttatgctt
     2701 ccaaaatatg tagaagattt actggaagag gaggaaaaca gtttatgagc agattattgg
     2761 taatcggatg tggtggggta gcttcagtgg caattcgcaa atgctgtcag aacagcgatg
     2821 tttttacaga gattatgatc gcaagcagga caaaggaaaa atgcgatgca ctcaaagaga
     2881 agattgaaag tacaacgaag acaaaaattg agacagcaaa ggtagatgcg gataatgcag
     2941 cagaggtcgc agaattgatc agagcctata agccggatgc tgtcctgaat gtggcacttc
     3001 catatcagga tcttaccatc atggatgcat gtctggaagc cggcgcggat tatattgata
     3061 ctgccaatta tgaagcagag gatacagaag atcccacatg gcgtgcaatc tacgaaaaga
     3121 gatgtaaaga aaaaggcttt actgcatatt tcgattattc ctggcagtgg gcttataatg
     3181 agaaatttaa ggaagcagga ctgactgctc ttctgggaac cggttttgat ccgggtgtta
     3241 ccagtgtatt cagtgcctat gccctgaagc attattttga tgaaatccat accattgata
     3301 ttctggactg taacggcggt gatcatggat atccttttgc cacaaacttt aacccggaga
     3361 tcaatcttcg agaggtttcc gcaaatggtt cctactggga agacggacat tgggtagaaa
     3421 cagagcctat ggaatttaaa tctgtctatg atttccctga ggtaggaaag aaggatatgt
     3481 atcttcttca tcatgaagag attgaatcac tggcaaaaaa tattccggga gtacagagga
     3541 tccgtttctt tatgacattc ggacagagct atctgacaca tatgaaatgt ctggaaaatg
     3601 taggaatgct ttccaccgca cctgtggaat ttaatggaca ggagatcgtg ccgatccagt
     3661 ttttgaaagc tcttctgccg gatcctgcat ctctgggtcc gagaacagtg ggtaaaacaa
     3721 atatcggctg tattttcaca ggtgttaagg acggaaaaga gaaaagtatt tatatttata
     3781 atgtatgtga tcatcaggaa tgctataaag aggtggaatc ccaggcaatc tcctatacaa
     3841 caggtgttcc ggctatgatc ggttccatga tggtagtgac cggacagtgg aagaagccgg
     3901 gtgtatttaa tgtggaagaa tttgatccgg atccatatat ggaagcactg aacaaatggg
     3961 gacttccatg gaaagtctgc gaagatcctg aactggtaaa ggtctggaaa gccaatgaag
     4021 attgatcaac tgcaaactcc ctgctatgtg atcgatgaga agaagatgcg ggagaatctg
     4081 gagattttga aaaaagtcca ggaggatacc gggtgtaaga tccttctggc gcagaaagcg
     4141 ttttccggct ttgctctgta tcccatgatc ggaaaatatc tggcaggtac cactgccagc
     4201 ggtctgtttg aggcaaggct tggatatgaa gagatgggga aagagaatca tgttttttcc
     4261 cctgcttacc gtacagaaga tatagaggaa ctggatcgta tctgtgacca tattatcttt
     4321 aattccacag cacagctgaa gaaatttaaa gaagtgtgta ccggagccag tctgggactc
     4381 cgtattaatc cggaatgctc cactcagggg gatcatgcga tctatgatcc ctgtgcgccg
     4441 ggttccaggc tgggggttac cagagagaac tttgacgggg aatgtctgaa atggatcgac
     4501 ggactgcatt ttcatacgct ctgcgaacag aatgcggatg acctggagaa aacattgcag
     4561 gcggtagagg agaagtttgg agaatttctg tctgaggttt cctggctgaa tatgggcggc
     4621 ggacatcata ttaccagaga ggattatgat gtaaagctcc tggaaaaatg tattcagcat
     4681 atgaaggaaa cctatgatct ggatatttat ctggagcccg gggaggctgt tgccctgaat
     4741 gccggatatc tggtgacaga ggttatggac attgtggaaa atgagatcag aacgctgatt
     4801 ttggatgctt ctgcagcctg ccatatgccg gatgtgctgg agatgcccta tcgtccgcct
     4861 cttaaggaca gcggggaagc aggggagaag gcatatacct accgcttatc ttcctgcacc
     4921 tgcctggcag gagatgtgat cggagattat tcctttgaca gagagatccg gatcggggac
     4981 aagctgtatt ttatggatat ggcgatttat tctatggtga agaataatac atttaacggt
     5041 atgccgctgc cggatattgc ggttatgcat gaggacggag agtgtgaggt tatcagacat
     5101 ttcggatacg aagactttaa gagccggctt tcctgatatc tgccgggcta tcaaagaaaa
     5161 ggagacagac atggaagtta cagagaagaa cagaacaaaa tatcgtatgc ccggagaatt
     5221 tgagcctcat gagggatgtg tgatgatctg gccggaacgt cccggttcct ggaattatgg
     5281 ggcaagggaa gcacagaaag catttgtgaa agttgcagag gctattggag tgagcgaaaa
     5341 ggtgtatatg ctggtttcca aagctcagat ggagaatgca aagaatcagc ttgggaatgt
     5401 ttccggtgtg actttgctgg aatgcgagac agatgacgcc tgggccagag atgtgggagc
     5461 aaccatggtt ctggatgaga aaggggcggt ctgtggagtt gactggcagt ttaatgcctg
     5521 gggaggtacc tttgacgggc tttaccggaa ctgggagaag gacgacaggg tggctgcctt
     5581 tatctgcagg actctcggtt gtccatgtct ggatgccaga ccttttgtgc tggagggcgg
     5641 ttccatccat tctgacggag aggggactct gattgttaca gaggcctgtc ttctgagtca
     5701 gggacgcaat cctcagatga gcagggaaca gatagaggaa cagttgaagt actggctcgg
     5761 tgtccacaag attatctggc ttccatgtgg aatctatcag gatgaaacca atgagcatgt
     5821 ggataacgtg tgcgcttttg taaggcccgg agaggttgtg cttgcatgga cagaggatga
     5881 gacagatccg cagtatgcca tgtctttggc tgatctgaag gtgctggaac aggaaacaga
     5941 tgcaaagggc agaaaattta agattcataa gcttccgatt cctcagaaac cggtctgcct
     6001 tacccaggaa gatgtggatg gctttgtgtt tgaagaggga gaagacgaac gggaagcagg
     6061 agaacggatg gctgccagtt acgtgaattt ctatatttcc aatggaggga tcatccttcc
     6121 acaatttggg gatgaaaatg acaaaagggc agtggagatt ctgcagaagt gttttcctga
     6181 aagaaggatt tatcccattg atgccagagc catcattgtc ggcggtggaa atattcactg
     6241 tattacccag cagattcccg gaaaaatatc tgaccttgat gcagaaaaac tttgagagtc
     6301 atgtgtttga gggttattac ctgaaagtta tgaaaaaact tacttatgta aacaggagca
     6361 ggaggaacag acaggatgag aaatgtaaca gtggcagcag ttcagatgaa atgcagtaaa
     6421 agtgtggaga aaaatattgc ccatgcagag gaactggtgc gtcaggcagc agcaaaggga
     6481 gctgagattg ttcttcttcc ggagcttttt gaacggccct atttctgcca ggagcgcaga
     6541 tatgagtatt atgaatatgc gcagactgca gaggaaaacc ctgcagtgcg tcatttcagc
     6601 agggtagcgg cagagctggg gattgtgatc ccggtcagct tttatgaaaa agaggtcaat
     6661 aatacctata acagcgttgc ggttctggat gcagatggaa agaatctcgg catttatcgg
     6721 aaaacacata ttccggatga ccattattat caggaaaaat tctatttcac accgggagat
     6781 accggcttta aggtttttga taccagattt ggaaccattg gagttggaat ctgctgggat
     6841 cagtggttcc cggaaacggc aagatgtatg gcactgcagg gagcagagct tctcttttat
     6901 cccactgcc
//
