LOCUS       c00164_gnlPro..         3808 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LBOIMAAL_164.
ACCESSION   c00164_gnlPro..
VERSION     c00164_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 01:59:24
            Original ID  :: gnl|Prokka|LBOIMAAL_164
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3808
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3808
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[472:3465]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      473..3465
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3808
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3808
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(473..1228)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 76.2;
                     E-value: 1e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg164_1"
                     /sec_met_domain="Radical_SAM (E-value: 1.5e-31, bitscore:
                     97.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDNAVKGFVHSIETFGSVDGPGVRFVIFLSGCAMRCQFCHNPDT
                     WKHDENTVEYTADELLNKALAYRPYWKNKGGITVSGGEPLLQIDFLLDLFKKAKKQGVN
                     TCIDTSANPFTTKEPFYSKWKELMEYTDLLLLDIKHIDNEQHKILTGQPNTNILEMARQ
                     LSDMGKPVWIRHVLVPERNDKDEYLFRLADFIRSLKNVERVEVLPYDTLGVYKWKALGY
                     DYPLEGINPPTKERVKNAEMILNGEKTRD"
     CDS             complement(1228..3465)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg164_2"
                     /sec_met_domain="Gly_radical (E-value: 2.1e-42, bitscore:
                     131.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.1e-272, bitscore:
                     892.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREEWTNFAGGKWESDIDVRDFIQKNYTPYDGDQSFLEGPTEATD
                     KLWGELKELQKEERAKGGVLDMETEVVSGITAYGAAYLDKDLEKVVGLQTDKPLKRAFM
                     PYGGIKMAEQSCQMYGYTPSPKLHEIFTKYHKTHNQGVFDIYTPEMKLARHNKILTGLP
                     DTYGRGRIVGDYRRVAVYGIDRLIEGKKEDFAETNRQGMRRGDFQLREEIADQIRALND
                     MKEMALSYGFDISQPAKNAAEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIYIERDL
                     ENGTLTESQAQELVDHFVMKCRMVKFARIESYNQLFSGDPVWATLEVAGLGIDGRSMVT
                     KNDYRFLHTLENMGPSPEPNLTVLYSSRLPENFKKYAAHISVTTSSIQYENDDVMRPVW
                     GDDYSICCCVSATQTGKEIQFFGARANLAKCLLYAINGGIDEKTKMQVGPEYTPITSEY
                     LDYDEVIKKYDTMMDWLAHLYVGTLNMIHYMHDKYYYEAAEMALIDTDVRRTFATGIAG
                     FSHVVDSLSAIKYAKVKVIRDEDGIAVDFETEGDFPRYGNNDERADKIAVDLLKTFMAK
                     LKYCHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANPSYGAEQNGLLA
                     SLNSVAKLPYEYALDGISNTQTINPGALGHNDDERVDNLVNVMDGYFDQGAHHLNVNVF
                     GTEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVIARTCHDRM"
ORIGIN
        1 atttttacaa atttttcaat aggcaactga taatctttgc acaaatggct gtcagtgtgt
       61 tatattctct ttttacgctc tgcaagggca aaaagcactc cgcccgaaat tacaacaagc
      121 atcataacag ccgttgctgt cactcctgtc tgaacactgc cgtttttatc aggctgtgaa
      181 ggagtgctgt ccgatgttgc ggcaccgcct gttgccgaag tcggatttgc agaggttgta
      241 ccgacagttg caggctcacc cgtcggagca gtctgaacag tctgttcctg agtagcagtc
      301 gtttcttccg acggtgacga tttttcaaca aggacagctt aaaaatcttt gaatacacct
      361 ttgattcgtc aattttgctg aataagaaac aacaaaagca gaataacaaa taaaaatcaa
      421 aacaaaaacg gcagtaccaa aaaaatcggt actgccgttg ttatataaga aattaatctc
      481 ttgttttttc gccgttaaga atcatttccg cattcttaac acgctctttt gtcgggggat
      541 taatgccctc gagaggatag tcataaccga gtgctttcca cttgtacaca ccaagtgtgt
      601 catacggaag aacttcaact ctttcaacat tcttgagcga acggataaag tcggcaagtc
      661 tgaaaagata ctcgtcctta tcatttcttt caggcacaag aacatgtctt atccatacgg
      721 gcttgcccat atcggaaagc tgtcttgcca tttcaagaat attggtgttt ggctgtcctg
      781 taaggatttt gtgctgttcg ttgtcgatat gctttatatc aagcaacagc aaatccgtat
      841 attccattag ctctttccac tttgaataga acggctcttt ggttgtaaac gggttggcgc
      901 ttgtgtcaat acaggtatta acgccctgct ttttagcctt cttgaacaaa tcaagcaaaa
      961 agtcaatctg taaaagcggc tcacctccgc ttactgtgat accgccctta tttttccaat
     1021 aggggcggta tgcaagcgct ttattaagca actcgtcggc ggtgtattcc acggtgtttt
     1081 catcgtgttt ccatgtgtcg ggattgtggc agaactggca acgcattgcg caaccgctca
     1141 gaaaaatcac aaatctcacg cccggaccgt caacagagcc aaaagtttct attgagtgta
     1201 caaagccttt aacagcgttg tccgacatta cattctgtcg tggcaggttc tggcaataac
     1261 atccagctgc tgctctcttg taaggtcgat gaacttaaca gcataacctg atacacggat
     1321 tgtgaagtta gcgtactctt ccttctcagg atgctccata gcgtcaatga gtttttctgt
     1381 accgaataca tttacattga ggtggtgtgc gccctgatca aagtagccgt ccataacatt
     1441 tacgaggttg tcaacacgct cgtcatcgtt gtgtccgaga gcacctgggt tgattgtctg
     1501 agtatttgaa ataccgtcaa gagcgtactc gtaaggaagc ttagcaacag agttaagtga
     1561 agcaagaaga ccgttctgct ctgcaccgta gcttgggtta gcacccggtg aaagcggctc
     1621 gcctgctttt ctgccgtcag gaagtgaacc tgtagcctta ccgtaaacaa catttgatgt
     1681 gattgtaagg atagaagttg taggctctga atctctgtac gtgtggcaat acttgagctt
     1741 agccatgaat gtcttgagaa gatcaacggc aatcttatct gctctctcgt cattgttacc
     1801 gtatcttggg aagtcgccct ctgtttcaaa gtcaacagca atgccgtcct cgtcacggat
     1861 aaccttaacc ttagcgtact taatagcgct gagtgagtca acaacatgtg agaagcctgc
     1921 aatacctgtt gcaaatgttc ttcttacatc tgtatcgata agagccattt ctgctgcctc
     1981 atagtagtac ttatcatgca tgtagtgaat catgttaagt gtgcctacat agaggtgagc
     2041 aagccaatcc atcattgtgt catacttctt gataacttcg tcataatcaa ggtactctga
     2101 tgtgataggt gtatattcag gaccaacctg catctttgtc ttttcatcaa taccgccgtt
     2161 gatagcgtag agaaggcact tagcaaggtt agctcttgcg ccgaagaact ggatttcctt
     2221 acctgtctgt gttgcggata cgcagcagca gattgagtaa tcatcgcccc atacaggacg
     2281 cattacatca tcgttctcgt actgaattga gcttgttgta actgaaatat gagcagcata
     2341 cttcttgaag ttctccggaa gtcttgaaga ataaagaact gtgaggtttg gctcaggtga
     2401 aggtcccata ttctcaagtg tgtggaggaa acggtagtcg ttctttgtaa ccattgaacg
     2461 gccgtcaatg ccaagacctg caacttcgag tgtagcccat acagggtcgc ccgagaagag
     2521 ctggttgtaa gactcaattc ttgcaaactt aaccatacgg cacttcataa caaagtggtc
     2581 aacgagttcc tgagcctgtg attctgtaag tgtaccgttc tcaaggtcac gctcgatgta
     2641 gatgtcgagg aatgttgata cacgaccaac gctcattgca gcgccgttct gagtcttgat
     2701 agcagcaaga taaccgaagt agagccactg aactgcctct gcagcgttct tagcaggctg
     2761 tgaaatatca aagccgtaag aaagtgccat ttccttcata tcgttaagag cacgaatctg
     2821 atcggcgatt tcttcacgaa gctggaagtc gcctcttctc ataccctgac ggtttgtttc
     2881 tgcaaagtcc tctttcttgc cttcgataag acggtcaata ccgtaaacag caactcttct
     2941 gtagtcaccg acgattctgc ctctgccgta tgtgtcggga agacctgtaa gaatcttgtt
     3001 gtgacgggca agcttcatct cgggagtgta gatatcgaat acaccctggt tgtgagtctt
     3061 gtgatatttt gtaaagattt cgtggagttt agggcttgga gtgtagccat acatctggca
     3121 tgactgctca gccattttga taccgccgta aggcataaat gcacgcttaa gaggcttgtc
     3181 tgtctgaaga cctacaacct tttcaaggtc tttatcaaga tatgcagcgc cgtaagctgt
     3241 gatgccgctt acaacctcag tttccatatc aagaacgccg cccttggctc tttcttcctt
     3301 ctggagttcc ttgagctcgc cccaaagctt gtctgtagct tctgtaggac cttcaaggaa
     3361 agactggtcg ccgtcataag gtgtgtagtt tttctgaatg aagtctctga catcaatgtc
     3421 ggactcccat ttgccacctg caaaatttgt ccattcttct ctcattactg taaaacctcc
     3481 gataatgata attaatactg aattttgtat atgacacaaa aaaatgccga ctcaattcca
     3541 agcagacgcc cggtaaggtc agctccttga aaaaatcgtc atacatcctt gtattctgtc
     3601 atttattcac ttttataatc atataaccaa caccattaaa ataacacttt ttaagggcgt
     3661 tgtcaagcat tttgagatac tttttacacc actttttcac actcaaaata ctatggtaaa
     3721 tgagtacaat tttagggtat gaggaacatt attttcagca gattttttcc tgtatttgtg
     3781 cggaatattt tgtcatatgc ctgctctg
//
