LOCUS       gnlProkkaCCFHKELF_16   14152 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CCFHKELF_16.
ACCESSION   gnlProkkaCCFHKELF_16
VERSION     gnlProkkaCCFHKELF_16
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 19:05:49
            Original ID  :: gnl|Prokka|CCFHKELF_16
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14152
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14152
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2540:4152]"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2541..4152
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
     cand_cluster    1..14152
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /kind="single"
                     /product="sactipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..14152
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="sactipeptide"
                     /region_number="1"
                     /rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /tool="antismash"
     CDS             3..167
                     /locus_tag="ctg16_1"
                     /transl_table=11
                     /translation="MFLHLKPDEYLKKLYKTKKLVEKTSFSLQAKDKARRLCPFDRDYI
                     FSGADTPIS"
     CDS             complement(131..1801)
                     /locus_tag="ctg16_2"
                     /transl_table=11
                     /translation="MNIAEQMKDVLKEEIKAAVLKAGLAEESQIPNVVLETPKDKTHGD
                     YSTNMAMQLARVAKKAPRQIAEEIVAHFDKGKASIEKLDIAGPGFINFYMNNQYLTKLI
                     PSVLEAGEAYGETNIGNGERVQVEFVSANPTGDLHLGHARGAAVGDSLCNVLSKAGYDV
                     SREYYINDAGNQINNLALSVEVRYFEALGLEKPMPEDGYRGEDIIAIGKRLAEEYGDRF
                     VNEEESERLAFFREYGLKYELEKLRKDLENFRVPFDVWYSETSLYQNGKIDKALEALRE
                     KGHVYEEDGATWFRSTTFGDDKDRVLIKKDGTYTYLLPDIAYHKDKLDRGFDKLINVWG
                     ADHHGYIPRMKAAIEALGYEKGTLEVEIIQLVHLYKNGEKMKMSKRTGKAVTMRDLIEE
                     VGLDAVRYFFAMRSADTHMDFDLDLAVSTSNENPVYYAQYAHARICSMLRQGEEQGLKP
                     AADLDFSHIQSEKEYDLLKTIGGFPEAVAEAAEKRIPHRVTNYIYDLASALHSFYNAEK
                     VIDPENEEKSRARLALMKATQITLNNALQLIGVSAPEKM"
     CDS             complement(1798..2226)
                     /locus_tag="ctg16_3"
                     /transl_table=11
                     /translation="MKQETPITLHVKSVIEDDGNQEVIEFRTTGFYYVKQNKVYLSYYE
                     EHDLGKVKTIVKVSEGEVLVMRSGAVKMNQRFVKGASTIAKYKMSFGELELKTSTKSIQ
                     SDLDEEKGRISIAYDMHVGDEQEHLHNMTITYEGGTHA"
     CDS             2541..2672
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: Subtilosin_A"
                     /gene_functions="biosynthetic-additional (sactipeptides)
                     predicted sactipeptide"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg16_4"
                     /sec_met_domain="Subtilosin_A (E-value: 9.8e-31, bitscore:
                     97.4, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKAVIVENKGCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
     CDS_motif       2541..2570
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKKAVIVENK"
                     /locus_tag="ctg16_4_sactipeptide"
                     /aSTool="sactipeptides"
                     /tool="antismash"
     CDS_motif       2571..2672
                     /prepeptide="core"
                     /locus_tag="ctg16_4_sactipeptide"
                     /peptide="sactipeptide"
                     /predicted_class
                     /score="33.00"
                     /molecular_weight="0.0"
                     /monoisotopic_mass="0.0"
                     /core_sequence="GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
                     /aSTool="sactipeptides"
                     /tool="antismash"
                     /leader_sequence="MKKAVIVENK"
                     /leader_location="[2540:2570](+)"
     CDS             2806..4152
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: subtilosin"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Subtilosin_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SPASM"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg16_5"
                     /sec_met_domain="subtilosin (E-value: 0.0, bitscore:
                     1081.4, seeds: 6, tool: rule-based-clusters)"
                     /sec_met_domain="Subtilosin_rSAM_RRE (E-value: 5.5e-31,
                     bitscore: 98.4, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 3.6e-09, bitscore: 28.4,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.2e-23, bitscore: 75.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 5.6e-12, bitscore:
                     37.5, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 1.8e-11, bitscore: 36.0,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFIEQMFPFINESVRVHQLPEGGVLEIDYLRDNVSISDFEYLDLN
                     KTAYELCMRMDGQKTAEQILAEQCAVYDESPEDHKDWYYDMLNMLQNKQVIQLGNRAIR
                     HTITTSGSNEFPMPLHATFELTHRCNLKCAHCYLESSPEALGTVSIEQFKKTADMLFDN
                     GVLTCEITGGEIFVHPNANEILDYVCKKFKKVAVLTNGTLMRKESLELLKTYKQKIIVG
                     ISLDSVNSEVHDSFRGRKGSFAQTCKTIKLLSDHGIFVRVAMSVFEKNMWEIHDMAQKV
                     RDLGAKAFSYNWVDDFGRGRDIIHPTKDAEQHRKFMEYEQNVIDEFKDLIPIIPYERKR
                     AANCGAGWKSIVISPFGEVRPCALFPKEFSLGNIFHDSYESIFNSPLVHKLWQAQAPRF
                     SEHCMKDKCPFSGYCGGCYLKGLNSNKYHRKNICSWAKNEQLEDVVQLI"
     CDS             4165..4326
                     /locus_tag="ctg16_6"
                     /transl_table=11
                     /translation="MSPAQRRILLYILSFIFVIGAVVYFVKSDYLFTLIFIAIAILFGM
                     RARKADSR"
     CDS             4323..5042
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 125.6; E-value:
                     4.3e-38)"
                     /gene_kind="transport"
                     /locus_tag="ctg16_7"
                     /transl_table=11
                     /translation="MSILDIHDVSVWYERDNVILEQVDLHLEKGAVYGLLGVNGAGKTT
                     LINTLTGVNRNFSGRFTLCGIEAEAGMPQKTSDQQKTHRYFAADYPLLFTEITAKDYVS
                     FVHSLYQKDFSEQQFASLAEAFHFSKYINRRISELSLGNRQKVVLMTGLLLRAPLFILD
                     EPLVGLDVESIEVFYQKTREYCEAGGTILFSSHLLDVVQRFCDYAAILHNKQIQKVIPI
                     GEETDLRREFFEVIGHE"
     CDS             5035..6345
                     /locus_tag="ctg16_8"
                     /transl_table=11
                     /translation="MNNIIPIMSLLFKQLYSRQGKKDAIRIAAGLVILAVFEIGLIRQA
                     GIDESVLRKTYIILALLLMNAYMVFLSVTSQWKESYMKLSCLLPISSRSFWLAQSVVLF
                     VDTCLRRTLFFFILPLFLFGNGTLSGAQTLFWLGRFSFFTVYSIIFGVVLSNHFVKKKN
                     LMFLLHAAIFACVCLSAALMPAATLPLCAVHMLWAVVIDFPVFLQAPPQQSRMHSFMRR
                     SEFSFYKREWNRFISSKAMLLNYAVMAAFSGFFSFQMMNTGIFNQQVIYIVISALLLIC
                     SPIALLYSIEKNDRMLLITLPIKGKTMFWAKYRFYSGLLAGEFLLVVMIVGFISGRPIS
                     VLTFLQCIELLLAGAFIRLTADEKRPFFSWQTEQQLWSGFSKYRSYLFCLPLFLATLAG
                     TAVSLAVIPIAGLVIVYYLQKQDGGFFDTSKRERLGS"
     CDS             6311..7495
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg16_9"
                     /sec_met_domain="Peptidase_M16_C (E-value: 5.8e-07,
                     bitscore: 21.2, seeds: 139, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIQAKENGLEVNLLKTHQFSTISIAASFLKPIESAAEPEEETIYF
                     YGAAAYLKEQIIDDFGCAAGSRFMYSANLFFDQQLKTCGTRLIHPLYNGNLHVDALMKT
                     FADLSFPSSLSFEAVEKARNELLLKIEKKFTDPFSYSAARLAEEVFGNPMYGTGMFGRK
                     DRIKAIHPKRFHDATDFIVDLVSQQKQLNILGQVQACDVRGHAPQTSAVTSERIPVNRH
                     VFETETRSAAGPSVLTLGFDCGEMKDASDYIKIQLIDGLLGKYGHSALFKHFREKDLAV
                     YHVITRYDVMNNLLLVSICTDQLHEIDIPPRVLEAVSAFHTDERELEQAKQFLRNELLL
                     QFDSPEGLLAYMGILRRFSCTKEALLDGISAVTCRDVLQFIATINYIGAHVVRG"
     CDS             7500..8780
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg16_10"
                     /sec_met_domain="Peptidase_M16 (E-value: 2.7e-15, bitscore:
                     48.2, seeds: 24, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKKAFFQQLDERTDIRYTDSGLKIFRLKFPRAHLRLCNVKIDFG
                     SRDVCIRAESGDTLLPYGTAHFLEHLLFWHNGRNLYSDFFAHGALLNAFTTYTDTNFMF
                     TSLPDRLRQTIPILLDALWNHSFDKKIVAQEKAVITSEIQTAHLNHQLSYHYQLISMLS
                     PSSPAAVFPAGRIEDIEALDISDLQKAYKAAYQTHRMTLFLIGGSENTETLLPPHLQLE
                     KRPDYHAERKIIPACPPALSQKMMLGDEERMEDTWTGLQIGALPGQNDLLTIKLYWDIA
                     ARILFQLDSPFFQEIQQTYRLEIDRLSAETYIYEDGGFLILHSQGTHSSAYIDVASYYV
                     TQKKEQAAAWLQYGKDSLTDAIIYDSDYVRKCFEWAAECDRCDCSFLDMYHIIQDMDAQ
                     VFLSLIDAMASSNKAIIHVSQKEAIRQ"
     CDS             8777..9478
                     /locus_tag="ctg16_11"
                     /transl_table=11
                     /translation="MKKNTVFTLLLLLIGMAAYSFGWVQAVAEAAAHYIQMINNDAVRL
                     GLLACTAALLMLPAFLYLHYVTQSVKNMTAAFQKLTQSHQSCCDFQQHNLCSRYAEDVK
                     SLRDGYKNVRQTYVMAAVLCQVIIFGCMFEIVKAVPFRLHTPPALSMGLAMLLILYLLF
                     CMRTYLRQLFRHGSLFRKVFAGVLAAAGIWWMLSFTISELLFLIILAAIQQIRSFIYKR
                     FSYRSTASLDL"
     CDS             complement(9484..10860)
                     /locus_tag="ctg16_12"
                     /transl_table=11
                     /translation="MKKNDLSALQENCFCFCDEEISREAPFQVPIDIPKGFKVDSAEAS
                     AAVTWSTDNLSCVSEPCLIQTGPETEDIGVRYAVRVQGTIMLLVSVSPVRNQYGQGDGA
                     VSVIHTEEIDQVVYYAAQSDRCPDFSQMTVEDLLIVPPFYGSPLTVAGTLVLPPSPDPQ
                     SYVFTANTGDSTVSVIDADLNTVVKTILFSAVPTNLGVTFDKAFTYVLHGNTNLVSVID
                     NKTLTIINTITVGGGPRKIEFDPTDEYAYVMAAGSIYMIDTANQSVIDVIAIPGALDFA
                     LDPNGQYVYTANGSSWSVDKYDVNTGQLVESIIDSFEFPSLIATPYAGNFAYVLNGELW
                     PKGVTEISLSPLSRGGDFNRLFETLRTIVFSLDSTRAYFLEPYSEPFLINNLYVVNTAR
                     QRIIANVPLPGAFDPAVTPDKQYIYAAQPNDNAVTVYRTSDYTAVKVIPVGAGPSAIVM
                     "
     CDS             complement(10899..11384)
                     /locus_tag="ctg16_13"
                     /transl_table=11
                     /translation="MFAYVLNFGLVQKVDLSTGEVTGTIERELIVSSIETDSPERYAYV
                     LEQEFLFNYLTVIDLNTFTISNTQELEPDGQYRMFTSGTGVYLYDDFTGNLYSVSPNGA
                     GVIGNLPQSATDYAFTPNGDFLYATRFIEQSIIVYNTDDYSEETVISLGVSPGAITI"
     CDS             complement(11417..12250)
                     /locus_tag="ctg16_14"
                     /transl_table=11
                     /translation="MRKNKLSFKEKAGAEQEECLCFCEGEASREAMFQAPIELPEGFVV
                     DPAEAVANVTWNADSLSCVSDRCLIQTGPEPDEVGVQFAVRLQGTVTLLVSVSPVRNQY
                     GQGDGAVSVIHNAEIDQVVYYSDEPDDCPDISNITIENLVVVPPFYGSPLSVTGTIVLV
                     PEPEPVYAFTANPNDQSVSVIDTNTHTVVTTIALPYNPAGIEITPDKSAVFVLHPNNNV
                     ISVIDYDTLTVTATILLDQPPRLIRFIPNHEFAYVFTGTAIYVIGIDTLTVERSI"
     CDS             12480..13625
                     /locus_tag="ctg16_15"
                     /transl_table=11
                     /translation="MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEE
                     DQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPRLSDLLLEIDKKQARLTGLLEYYFN
                     FFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFSMDY
                     ARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAVSHFQKAYSMAEAEKQPQLMG
                     RTLYNIGLCKNSQSQYEDAITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKA
                     HEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAIQGFFDFLESKMLYADLEDFT
                     IDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV"
     CDS             13609..13728
                     /locus_tag="ctg16_16"
                     /transl_table=11
                     /translation="MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI"
     CDS             13826..14137
                     /locus_tag="ctg16_17"
                     /transl_table=11
                     /translation="MSLKSVRTHFTQWNRENDVTEFETSSATVEQAAETIGVSLSRIAK
                     SLSFRGEGDQVILIVAAGDAKIDNKKSRQTFGFKARMLSPNEVLEQTGHEIGGVCPIG"
ORIGIN
        1 ctctgtttct gcatctgaaa cctgatgaat atctaaagaa actgtataaa acaaaaaagc
       61 ttgtagaaaa aacgtcgttt tctctacaag ctaaggacaa agcccgaaga ctttgtcctt
      121 ttgatcgtga ttacattttt tccggagccg atacgccgat cagctgaagc gcattgttca
      181 gcgtaatttg cgttgccttc attaaagcca ggcgcgcacg gcttttctct tcattttcag
      241 ggtcgatgac tttctctgcg ttgtagaagc tgtgcagagc agaggccaga tcataaatat
      301 agtttgtgac acggtgcgga attcgttttt ccgctgcttc cgccactgcc tctgggaagc
      361 cgccgatcgt tttcagcaga tcgtattctt tttctgattg aatatggctg aaatcaagat
      421 ccgccgccgg tttaagtccc tgctcttccc cttggcgaag catgctgcag attcgggcat
      481 gtgcatattg tgcataatac acagggtttt cgttagatgt tgatacagca agatctaagt
      541 caaaatccat atgggtgtcc gcacttcgca tcgcaaagaa gtaacgaacg gcatccaagc
      601 cgacttcttc aatcaggtca cgcatcgtta cggctttacc ggtccgtttg ctcattttca
      661 ttttctcgcc gtttttgtaa aggtgaacga gctgaatgat ttctacttca agcgttcctt
      721 tttcatagcc cagcgcttcg attgccgctt tcatacgcgg gatgtagccg tggtgatcgg
      781 cgccccaaac gttgatcagc ttatcaaagc cgcggtcaag cttgtcctta tgatacgcga
      841 tatcaggaag caggtacgtg tacgtgccgt ccttcttgat caatacgcgg tctttatcat
      901 cgccaaacgt cgtggaacgg aaccaagtcg cgccgtcttc ttcatagaca tggccttttt
      961 cgcggagcgc ttcaagcgcc ttgtcgattt ttccattttg gtacagtgaa gtttctgaat
     1021 accacacatc aaacggcaca cggaagtttt ccaagtcctt gcgaagcttt tccagctcat
     1081 atttcaggcc gtattcacgg aaaaacgcga ggcgttcact ttcttcttcg ttaacgaaac
     1141 gatcaccgta ttcctcagcg agacgctttc cgatcgcaat gatatcctcg ccgcggtagc
     1201 catcctccgg catcggtttt tccaatccga gtgcttcaaa gtaacggact tcaacagaaa
     1261 gcgccagatt gttgatttgg ttacccgcat cgttaatgta gtattcgcgg cttacatcgt
     1321 aacccgcttt tgaaagcacg ttgcacaagg aatcgccgac agccgccccg cgcgcatgcc
     1381 cgaggtgaag gtctcccgtc ggattcgctg atacgaattc tacctgaact cgttcaccgt
     1441 ttccgatgtt cgtttccccg tatgcttccc ctgcttccag tacagacgga atcagctttg
     1501 ttaaatattg attgttcata tagaaattga taaaacccgg gccggcgata tccagttttt
     1561 caatcgaagc ctttccttta tcaaaatggg cgacaatctc ttcagcgatt tggcgcggcg
     1621 ctttttttgc cactcttgcc agctgcatcg ccatattcgt tgagtagtcg ccgtgtgttt
     1681 tatctttcgg tgtttctaaa acaacattgg gaatctggct ttcttcagcc agtcccgctt
     1741 tcagaacggc cgctttgatt tcttctttca gcacgtcctt catttgttcc gcaatgttca
     1801 tgcgtgagtt cctccctcat acgtgatcgt catattatgt aaatgctcct gctcgtctcc
     1861 gacatgcatg tcatatgcga tgctgatacg gcctttttct tcatcgagat cagattgaat
     1921 cgattttgtg cttgttttca gctcaagctc accaaatgac attttatatt tagcaattgt
     1981 ggaagctccc tttacgaaac gctgattcat tttgacagcg cctgacctca tgaccaggac
     2041 ttcgccttcg cttactttca caatcgtttt gactttgcct aagtcgtgtt cttcataata
     2101 agatagatac actttatttt gttttacata gtaaaatccg gtggtgcgga attcgatgac
     2161 ttcttggttt ccgtcatcct cgatcacaga cttcacatgg agtgttatcg gtgtctcttg
     2221 cttcattctg acacgtcctt ttcatcattc cactttgact tgaatagtat aatcattcgg
     2281 tttgtaaact tcaactgctt ctatcttacc atcattgctc atcagatttg aagataaacc
     2341 tcataaaaag catttcctta tatagaagag aaaatcatat cactaattac ctttagtaaa
     2401 tgttacattt ttccgaaatc tatcatttcc ttttcacatt tttttcaaaa tatatgtatt
     2461 gaattagtaa tttgatagtt ttaagataaa agtacaacat agatctgtta gaaaaacaaa
     2521 aaaaaaggga ggattcaatt atgaaaaaag ctgtcattgt agaaaacaaa ggttgtgcaa
     2581 catgctcgat cggagccgct tgtctagtgg acggtcctat ccctgatttt gaaattgccg
     2641 gtgcaacagg tctattcggt ctatgggggt aaggatctcc caaaagggca tagtcattct
     2701 actatgccct ttttaagtaa attacaattt ttaactaaaa tctacctctt ggatatacat
     2761 acacaaccgt ttttcatttg aatcatatgt ataggggatg gtattttgtt tatagagcag
     2821 atgtttccat ttattaatga aagtgtaaga gttcaccagc ttcctgaggg cggcgtgtta
     2881 gaaatcgact acttgcgcga taatgtctcc atttctgact ttgagtattt ggatctcaac
     2941 aaaacagctt acgagctctg catgcgcatg gatggccaaa aaacagctga gcagatttta
     3001 gctgagcaat gtgcagtgta tgatgaatca ccggaagacc ataaagattg gtattacgac
     3061 atgctcaaca tgctccagaa caagcaggtt atccagcttg gaaaccgggc catccgccat
     3121 accatcacca cgagcggaag caatgaattt ccgatgcccc tgcacgccac ctttgaactg
     3181 acgcaccgct gtaatttgaa atgcgcccac tgttatttgg aaagctcgcc tgaagcgctc
     3241 ggcaccgtgt cgattgagca attcaaaaaa acggctgata tgctgtttga taacggtgta
     3301 ttgacatgcg aaatcacagg cggagaaatt tttgtccatc caaacgccaa tgagattctt
     3361 gactatgtgt gtaaaaagtt caaaaaagtc gctgtcttaa caaacggaac actcatgcga
     3421 aaagagagcc tggagctttt gaagacttac aagcaaaaaa tcatcgtcgg catttctctt
     3481 gatagtgtca attccgaggt ccatgactcc tttagaggga gaaaaggctc ttttgcccaa
     3541 acttgtaaaa cgataaaatt gttgagtgac cacggtatat ttgtcagagt cgccatgtct
     3601 gtcttcgaaa aaaacatgtg ggaaatccac gatatggccc aaaaggttcg ggatctcgga
     3661 gcgaaggcgt tttcttacaa ttgggttgac gatttcggaa gaggcaggga tattatccat
     3721 ccaacgaaag acgccgagca gcaccgcaag tttatggaat acgagcaaaa tgtgattgat
     3781 gagtttaaag atctgattcc gattattccc tatgagagaa aacgcgcggc aaattgcggc
     3841 gctggctgga agtccattgt gatcagcccg ttcggcgaag tacgtccttg cgccctcttt
     3901 ccaaaggaat tttcattggg aaatattttt catgattcct atgaaagcat ctttaactcc
     3961 cctctcgtcc ataaactgtg gcaagcgcag gcgccgcggt tcagcgaaca ttgcatgaaa
     4021 gacaaatgcc cgttcagcgg ctattgcgga ggctgttact taaaagggct gaactctaac
     4081 aaatatcacc gaaaaaacat ttgctcttgg gcgaaaaatg aacaattaga agacgtggtc
     4141 cagcttattt aggagggaaa cgatttgtca ccagcacaaa gaagaatttt actgtatatc
     4201 ctttcattta tctttgtcat cggcgcagtc gtctattttg tcaaaagcga ttatctgttt
     4261 acgctgattt tcattgccat tgccattctg ttcggaatgc gcgcgcggaa ggctgactcg
     4321 cgatgagcat tttggatata cacgatgtat ccgtttggta tgaacgggac aacgtcatct
     4381 tagaacaagt ggatttacat ttagaaaaag gcgccgttta cgggctgctt ggggtaaacg
     4441 gcgccggaaa aacgacactg attaatacgc tgacaggcgt gaaccgcaat ttcagcgggc
     4501 gctttacact gtgcggaatt gaagcggagg ccggcatgcc gcagaaaaca tcagatcagc
     4561 agaagactca ccgttacttc gccgccgatt atccgctgct atttacagaa attacggcga
     4621 aggactatgt gtctttcgtc cattcgcttt atcaaaagga tttttcagag cagcagtttg
     4681 ccagtttggc cgaggccttt catttttcga aatacatcaa caggagaatt tcggagctgt
     4741 ccttggggaa caggcaaaag gttgtattga tgacaggctt attgctgcgc gctcccctat
     4801 ttattttgga tgagccgctc gtcggtttgg atgtggaatc gatagaggtt ttttatcaga
     4861 aaacgcgcga gtactgtgaa gcaggcggga ccattttgtt ttcttctcat ctgctcgatg
     4921 tcgtgcaaag attttgtgat tacgcggcca ttcttcacaa caaacagatc caaaaggtca
     4981 ttccgattgg agaggagacc gatctgcggc gggaattttt cgaggtgatc ggccatgaat
     5041 aacataatcc ccatcatgtc tttgctgttc aaacagcttt acagccggca agggaaaaag
     5101 gacgccatcc gcattgccgc aggccttgtc attctggccg tatttgaaat cgggctgatc
     5161 cgccaggccg gcattgatga atcggtgttg cgcaaaacgt atatcatact cgcgcttctc
     5221 ttaatgaacg catatatggt gtttctttct gtgacatctc aatggaagga atcttatatg
     5281 aaactgagct gcctgctgcc gatttcttca cggagctttt ggctcgccca gagtgtcgtt
     5341 ttgtttgtcg atacctgttt gagaagaacg ttattctttt ttattttacc gctgttctta
     5401 tttggaaacg gaacgctgtc aggggcgcaa acattgtttt ggctcggcag gttttcgttt
     5461 tttaccgttt actccattat tttcggagtt gtgctaagca accacttcgt caaaaagaag
     5521 aacttgatgt ttctgctgca tgcggcgata ttcgcctgtg tatgtctcag cgccgctttg
     5581 atgccggccg ccacgcttcc gctttgcgcg gtgcatatgc tgtgggcggt ggtcattgac
     5641 tttcctgtct ttctgcaggc gcctccgcag cagagcagga tgcattcatt tatgcggcga
     5701 tctgagtttt cgttttacaa aagagaatgg aaccgattta tctcttctaa agcgatgctg
     5761 ttaaattacg cggtaatggc ggcgttcagc ggcttctttt cgttccagat gatgaatacc
     5821 ggcatcttca atcagcaagt gatttatatc gtgatttccg cgcttctgct catctgctcg
     5881 ccgatcgccc ttttgtactc gattgaaaaa aatgaccgga tgctgctcat cacgcttccg
     5941 atcaagggga aaacgatgtt ttgggcgaaa tatcgctttt attcaggcct attggcaggc
     6001 gaatttctcc ttgtcgtgat gattgtgggt ttcatcagcg gccgtccgat ttcagttctc
     6061 acttttttgc agtgcattga gttgctgctg gcgggagctt ttatcaggtt aactgcggat
     6121 gaaaaaaggc ccttcttcag ctggcagacg gagcagcagc tatggagcgg tttctcaaaa
     6181 tatcggtctt atctgttttg tcttccccta ttcttagcca cattagccgg caccgctgta
     6241 tccttggcgg tcattccgat agccggtctg gtcatagtct attacctgca aaaacaggat
     6301 ggtggtttct ttgatacaag caaaagagaa cggcttggaa gttaacctgc tgaagactca
     6361 tcaattttca actatatcaa tcgccgcgag ctttctgaaa ccgatcgaaa gcgcggcgga
     6421 acctgaggaa gaaacgattt atttttacgg cgccgccgca tacctgaagg agcaaatcat
     6481 tgacgatttc ggttgtgctg ccggcagccg tttcatgtac tcagcgaatc tcttttttga
     6541 tcagcagctg aaaacctgcg ggacccgttt gattcaccca ttatataatg ggaatcttca
     6601 tgttgacgcc ctgatgaaaa cttttgcaga cttgtctttc ccttcttctc tttcattcga
     6661 agcagttgaa aaggccagga atgagctgct gctgaaaatc gaaaagaaat tcactgatcc
     6721 cttctcctac tcggcggcgc gcttggctga ggaggtcttc ggaaacccga tgtacggcac
     6781 aggcatgttt ggccggaaag acaggataaa ggcgattcat cctaaacggt ttcacgacgc
     6841 aacggacttt atcgttgatt tagtgtccca gcaaaaacag ctgaacatat tgggacaagt
     6901 acaagcatgt gatgttcggg gacatgcccc gcaaacgtcc gctgtaacgt ccgaaagaat
     6961 acccgtaaac cggcatgttt ttgaaacaga gacacgcagc gctgcaggtc cttccgtctt
     7021 gacactcgga tttgactgcg gagagatgaa agatgccagc gattatatca aaatacagct
     7081 aatagacgga ttactgggaa agtacggcca ttccgcttta ttcaagcact tcagagagaa
     7141 agatctggca gtctatcacg ttatcacccg ttacgatgtc atgaacaatt tgctgcttgt
     7201 ttcaatatgt acagatcagc ttcatgagat agacattccc ccgcgcgttt tggaagctgt
     7261 ctcagctttt catacagatg agcgggaact agaacaggca aagcagtttt tacgcaatga
     7321 attgctgctg caattcgatt ctcccgaagg tctattggcc tatatgggga tcctgcgccg
     7381 cttctcctgc acgaaagaag ccttgctgga tggcatctca gcggttacat gccgtgatgt
     7441 attgcaattc atagcgacca tcaattatat cggagctcat gtagtaaggg gatgaaaaga
     7501 tggaaaagaa agcctttttt caacagctgg atgaacgaac cgatatcaga tacacagaca
     7561 gcggactaaa aatattcaga ttgaaattcc cccgcgccca cctgcggctg tgcaatgtaa
     7621 aaatcgattt tggaagccgg gatgtatgta tacgggcaga atccggtgat acactgcttc
     7681 cgtatggcac agcgcatttt ttagagcatc ttctcttctg gcataacggc cgcaatctgt
     7741 acagtgattt ttttgcgcat ggcgcgcttt taaacgcatt cactacctac accgatacca
     7801 atttcatgtt tacctcatta ccggatcggc tcagacaaac gatccccatc ctgcttgatg
     7861 ctttatggaa ccactcattt gacaagaaga ttgtcgctca ggaaaaagct gtgattacaa
     7921 gcgaaattca aacagcacac cttaaccatc agctctcata tcattatcag ctgatcagca
     7981 tgctttctcc ctcctcacca gctgccgttt tccccgcagg cagaatcgaa gatattgagg
     8041 cgcttgacat cagtgattta caaaaagcct ataaggctgc ctaccaaacc catcggatga
     8101 cgctgttttt gattgggggc tcagaaaata cagaaacgct attgcctcct cacctccagc
     8161 tggaaaagcg tccggattat catgctgaaa gaaaaatcat cccggcatgc cctccagccc
     8221 tctcacaaaa aatgatgctg ggtgatgagg agcggatgga agatacatgg accggattac
     8281 agatcggcgc acttcccggg caaaacgatc tgttgacgat caaactttat tgggatatcg
     8341 cggctcgtat tctatttcaa ttagattcac cgttttttca agagattcag caaacgtacc
     8401 gcctggaaat cgatcgtttg tcagccgaga cttatatcta tgaggatggc ggtttcttga
     8461 tccttcacag tcaagggacc cattcttcag cttatatcga tgtggcttca tactatgtca
     8521 cacagaagaa agaacaggct gccgcctggc ttcaatacgg caaggactct ttgacagacg
     8581 cgatcattta tgacagcgat tatgtgcgta aatgcttcga gtgggcagcc gagtgtgaca
     8641 ggtgcgattg ctcgtttctt gacatgtacc acatcattca agatatggat gcgcaggttt
     8701 tcttgtctct cattgatgca atggcatctt ctaacaaagc gatcattcac gtttctcaga
     8761 aagaggcgat cagacaatga aaaaaaacac tgtctttacc cttttattgc tgcttatagg
     8821 tatggcggct tattccttcg gctgggttca ggccgttgcc gaagccgccg cccactatat
     8881 acagatgatc aacaatgatg ctgtacggct cggactgctg gcttgtaccg ctgccctcct
     8941 gatgctgcct gcttttctct atcttcacta tgtcacccaa tctgtgaaaa acatgacagc
     9001 agcttttcag aaactcacac agtctcatca aagctgctgt gactttcagc aacacaactt
     9061 gtgcagccga tatgcagaag acgtcaaatc gctgcgtgac ggctataaaa acgtccgcca
     9121 aacatacgtg atggctgccg tgttgtgcca agtcatcata ttcggctgca tgttcgaaat
     9181 cgttaaagcc gtccctttcc gccttcacac gccccctgcc ctttcaatgg gacttgcgat
     9241 gctactgatc ctgtatttat tgttttgcat gcgaacgtat ttgcggcagc ttttccggca
     9301 cggcagcctg ttcagaaaag tgtttgcggg agtgctggca gcagccggga tttggtggat
     9361 gctcagtttc actataagtg aactgctgtt tctgattatc ctggctgcca tccagcaaat
     9421 cagatcattt atttataagc gattctctta tcgcagcacc gcttctcttg atctataaaa
     9481 tagctacatc acaatcgcag acgggcccgc gccaaccgga atcaccttca cggctgtata
     9541 atcacttgtg cggtacacag tcacagcatt gtcgttgggc tgtgccgcat aaatatactg
     9601 tttgtccggc gttacggcgg ggtcaaaagc gccggggaga ggcacgtttg caatgatcct
     9661 ctgtctggcc gtattaacta cgtataagtt attaatgaga aacggttcac tatatggctc
     9721 tagaaaataa gctctagtac tatcgagaga aaatacaatc gtcctcagcg tttcaaataa
     9781 acggttaaag tcccctcccc tgcttaatgg tgacaaacta atctccgtta cacctttcgg
     9841 ccacagctca ccattcaaca cgtacgcaaa atttccagcg taaggagttg caattaaact
     9901 gggaaattca aatgaatcta tgatactttc taccaactgc ccagtattta catcatattt
     9961 atcaaccgac caactgcttc cattagcggt atagacatac tgtccattcg gatcaagagc
    10021 aaagtctaaa gcgccgggta tcgcgattac atcaattact gattgattgg ccgtgtcaat
    10081 catatatata gatccggcgg ccatcacgta ggcgtactca tctgttggat caaattcgat
    10141 ctttctcggt cctcctccaa cagtaatggt atttatgatg gttaatgttt tattatcaat
    10201 cactgagaca aggttggtat tcccatggag gacatacgtg aatgctttat cgaaagtaac
    10261 gcctagatta gttggcacag cagaaaacag gattgttttt acaacagtgt ttaaatctgc
    10321 atcaatgacc gaaaccgtgc tgtctccagt attggcagta aacacatacg attgagggtc
    10381 tggcgatggc ggcaaaacaa gtgtcccggc caccgtgagc ggactgccgt agaaaggcgg
    10441 cacgatgaga agatcttcca ctgtcatctg actaaaatca ggacagcggt cagactgtgc
    10501 tgcataataa acaacttgat caatctcttc agtatgaatc acagaaaccg ccccgtctcc
    10561 ttgtccatat tgatttcgca caggcgacac actgacaaga agcataatgg tcccttgtac
    10621 tctcacagca tatcgaacac caatatcttc tgtttccggc ccagtctgga ttaagcaagg
    10681 ctcactgaca caggacaaat tgtctgtact ccatgtaaca gccgctgacg cttcggctga
    10741 atcaaccttg aagcccttag ggatatctat tggcacttga aacggcgctt cccggctaat
    10801 ttcctcatca caaaaacaga aacaattttc ctgtaaagca gaaagatcat tctttttcat
    10861 gaattcacgt ccttttaaaa atgcacaaca tgcagcggtt aaatcgtaat ggcacccggt
    10921 gaaaccccaa gagatatcac agtttcctca gaataatcat ctgtgttgta aacaatgatg
    10981 ctctgctcta taaaacgagt tgcatacaga aaatcgccat tcggggtaaa cgcatagtct
    11041 gttgctgatt gcggaagatt tccaattaca cctgctccat tcggactgac agaatataaa
    11101 ttgccggtaa agtcgtcata taaatacaca cctgttccgc tcgtaaacat tctatattgg
    11161 ccgtcaggct ccagttcttg ggtattgctg atcgtaaacg tatttaagtc aataaccgtc
    11221 aaataattga ataagaattc ttgttctaag acatacgcat accgctccgg agaatctgtt
    11281 tctatggatg atacgataag ctctcgttca atcgttcctg tgacttcacc ggtggataag
    11341 tccacttttt gcactaaccc aaaattaaga acataggcga acaacccatt ggggtcaatt
    11401 gcaatatcaa aaccttctat atagacctct caacagtcaa cgtatcaatt ccaatcacat
    11461 aaatggcagt accggtgaaa acataagcaa actcatgatt agggataaat ctaatcaatc
    11521 gaggcggctg atccagcaat atagttgctg tcactgttaa tgtgtcataa tcgatgacag
    11581 aaatcacatt attgttggga tgtaagacaa acacagcgct tttatctggc gtaatttcaa
    11641 tacctgccgg attgtacgga agagcaatcg ttgtcacaac agtatgggtg tttgtatcaa
    11701 tcacagaaac tgattgatca tttggattcg ctgtaaacgc gtagacgggt tccggctccg
    11761 gtacaagcac aatcgtcccc gtcaccgata aaggactgcc gtaaaacggc ggaacaacaa
    11821 caaggttctc aatcgtgatg ttactgatat ccgggcaatc atccggttca tcggaataat
    11881 agacgacttg atcgatttct gcattgtgaa tgacggaaac agccccatcc ccttgtccat
    11941 attgatttcg cacaggtgag acactgacga gcagcgtaac ggttccctgc agacgcactg
    12001 caaattgaac accgacctca tcaggctctg gccccgtttg aattaagcaa cggtcactga
    12061 cacaagaaag gctgtctgca ttccacgtca cgtttgcaac agcttcagcc ggatcaacga
    12121 caaatccttc aggtaattca atcggcgctt gaaacatggc ttctcggctt gcttctccct
    12181 cacaaaaaca taagcactct tcttgttctg ctcctgcttt ttctttaaag gaaagcttgt
    12241 tttttctcat tccttcactt ccttttcaga catacactgc tcacgcagat ataacagaat
    12301 atggaagagt aacgtgtttg cttgtacaaa ttactgaggg taaaagcttc tttttgaaaa
    12361 tatatctcca aacatgcggt gtaacgaaag aaaaaattgc ggtttttttg cgcttctcac
    12421 aagaatgttg atgataaaat atgacatata tagaattatg aagggaggga tttgcaaacg
    12481 tgacaggtgt catatcttct tcttccatcg gagaaaagat taacgaatgg tatatgtaca
    12541 tacgccgatt cagcataccc gatgcagaat atttgcgacg agaaatcaag caagagctgg
    12601 atcaaatgga agaagatcaa gaccttcatt tgtactattc actgatggag tttcggcaca
    12661 acctgatgct tgagtacctt gaaccgttag aaaaaatgag gattgaggaa cagccgagac
    12721 tgtctgatct gctgcttgag attgataaaa aacaggctcg tttaacaggt ctgcttgagt
    12781 actattttaa tttcttcaga ggcatgtatg agctggatca gcgggaatat ctgtcggcta
    12841 ttaaattttt caaaaaggcc gaaagcaagc tgatattcgt taaggatcgg atagagaaag
    12901 ctgagttttt ctttaagatg tctgaatctt attactatat gaaacaaacg tatttttcaa
    12961 tggactatgc acggcaagca tatgaaatat acaaagaaaa tgaagcttat aatataagat
    13021 tgctgcagtg tcattcttta tttgccacca attttttaga tttaaaacag tatgaggatg
    13081 ccgtctcaca ttttcaaaaa gcttattcta tggcagaagc tgaaaagcag ccccaattaa
    13141 tgggaagaac tttgtacaat atcgggcttt gtaaaaacag ccaaagccaa tatgaggatg
    13201 ccataacata tttcaaaaga gcaatagctg tttttgaaga atcaaatatt cttccttcct
    13261 tacctcaagc gtatttttta attacacaga tccattataa attaggaaaa atggataaag
    13321 ctcatgaata tcatagtaag ggaatggctt attcacaaaa ggccggagat gtaatatatt
    13381 tatcagagtt tgaatttttg aaatctttat acttatcagg cccggatgaa gaagcaattc
    13441 aaggattttt tgattttctc gaaagtaaaa tgttgtatgc tgatcttgaa gatttcacta
    13501 ttgatgtggc aaaatattat catgaacgta aaaattttca aaaagcttct gcttattttt
    13561 tgaaggtgga acaagtaagg caacttattc aaggaggagt gagtttgtat gaaattgaag
    13621 tctaaactat tactctcttg tctggctcta agcactgtgt tcgtggcaac aactattgca
    13681 aatgcaccta cacaccaaat tgaagttgca caacgaggaa tgatttaacc gccgtccatc
    13741 ggcggttttt ttgtcccctc tttacccagt ctcccaatcc attctatgat tttttcaata
    13801 atcttaaaaa tagtggaaat gcagcatgtc tctaaaaagt gtgagaaccc actttactca
    13861 atggaatcga gaaaatgatg tgacggagtt cgaaacgtcg agtgcgacag ttgaacaggc
    13921 agctgagaca atcggcgtaa gcctgtctag aatcgccaag tccctgtcct tcagagggga
    13981 aggagatcag gtgattctga ttgtggcagc cggcgatgcc aagatcgaca acaaaaagtc
    14041 caggcaaaca tttggcttta aagcaagaat gctctctcct aatgaggtac tggagcagac
    14101 aggccatgaa attggaggag tttgcccaat tggatagact catgatcctg ag
//
