LOCUS       gnlProkkaAPGMFHAH_7    14227 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|APGMFHAH_7.
ACCESSION   gnlProkkaAPGMFHAH_7
VERSION     gnlProkkaAPGMFHAH_7
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 19:57:55
            Original ID  :: gnl|Prokka|APGMFHAH_7
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14227
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14227
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2615:4227]"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2616..4227
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
     cand_cluster    1..14227
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /kind="single"
                     /product="sactipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..14227
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="sactipeptide"
                     /region_number="1"
                     /rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /tool="antismash"
     CDS             69..242
                     /locus_tag="ctg7_1"
                     /transl_table=11
                     /translation="MGALFLHLKPDEYLKKLYKTKKLVEKTSFSLQAKDKARRLCPFDR
                     DYIFSGADTPIS"
     CDS             complement(206..1876)
                     /locus_tag="ctg7_2"
                     /transl_table=11
                     /translation="MNIAEQMKDVLKEEIKAAVLKAGLAEESQIPNVVLETPKDKTHGD
                     YSTNMAMQLARVAKKAPRQIAEEIVAHFDKGKASIEKLDIAGPGFINFYMNNQYLTKLI
                     PSVLEAGEAYGETNIGNGERVQVEFVSANPTGDLHLGHARGAAVGDSLCNVLSKAGYDV
                     SREYYINDAGNQINNLALSVEVRYFEALGLEKPMPEDGYRGEDIIAIGKRLAEEYGDRF
                     VNEEESERLAFFREYGLKYELEKLRKDLENFRVPFDVWYSETSLYQNGKIDKALEALRE
                     KGHVYEEDGATWFRSTTFGDDKDRVLIKKDGTYTYLLPDIAYHKDKLDRGFDKLINVWG
                     ADHHGYIPRMKAAIEALGYEKGTLEVEIIQLVHLYKNGEKMKMSKRTGKAVTMRDLIEE
                     VGLDAVRYFFAMRSADTHMDFDLDLAVSTSNENPVYYAQYAHARICSMLRQGEEQGLKP
                     VADLDFSHIQSEKEYDLLKTIGGFPEAVAEAAEKRIPHRVTNYIYDLASALHSFYNAEK
                     VIDPENEEKSRARLALMKATQITLNNALQLIGVSAPEKM"
     CDS             complement(1873..2301)
                     /locus_tag="ctg7_3"
                     /transl_table=11
                     /translation="MKQETPITLHVKSVIEDDGNQEVIEFRTTGFYYVKQNKVYLSYYE
                     EHDLGKVKTIVKVSEGEVLVMRSGAVKMNQRFVKGASTIAKYKMSFGELELKTSTKSIQ
                     SDLDEEKGRISIAYDMHVGDEQEHLHNMTITYEGGTHA"
     CDS             2616..2747
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: Subtilosin_A"
                     /gene_functions="biosynthetic-additional (sactipeptides)
                     predicted sactipeptide"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg7_4"
                     /sec_met_domain="Subtilosin_A (E-value: 1.5e-30, bitscore:
                     97.4, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKAVIVENKGCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
     CDS_motif       2616..2645
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKKAVIVENK"
                     /locus_tag="ctg7_4_sactipeptide"
                     /aSTool="sactipeptides"
                     /tool="antismash"
     CDS_motif       2646..2747
                     /prepeptide="core"
                     /locus_tag="ctg7_4_sactipeptide"
                     /peptide="sactipeptide"
                     /predicted_class
                     /score="33.00"
                     /molecular_weight="0.0"
                     /monoisotopic_mass="0.0"
                     /core_sequence="GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
                     /aSTool="sactipeptides"
                     /tool="antismash"
                     /leader_sequence="MKKAVIVENK"
                     /leader_location="[2615:2645](+)"
     CDS             2881..4227
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: subtilosin"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Subtilosin_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SPASM"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg7_5"
                     /sec_met_domain="subtilosin (E-value: 0.0, bitscore:
                     1081.4, seeds: 6, tool: rule-based-clusters)"
                     /sec_met_domain="Subtilosin_rSAM_RRE (E-value: 8.2e-31,
                     bitscore: 98.4, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 5.4e-09, bitscore: 28.4,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.7e-23, bitscore: 75.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 8.4e-12, bitscore:
                     37.5, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 2.6e-11, bitscore: 36.0,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFIEQMFPFINESVRVHQLPEGGVLEIDYLRDNVSISDFEYLDLN
                     KTAYELCMRMDGQKTAEQILAEQCAVYDESPEDHKDWYYDMLNMLQNKQVIQLGNRAIR
                     HTITTSGSNEFPMPLHATFELTHRCNLKCAHCYLESSPEALGTVSIEQFKKTADMLFDN
                     GVLTCEITGGEIFVHPNANEILDYVCKKFKKVAVLTNGTLMRKESLELLKTYKQKIIVG
                     ISLDSVNSEVHDSFRGRKGSFAQTCKTIKLLSDHGIFVRVAMSVFEKNMWEIHDMAQKV
                     RDLGAKAFSYNWVDDFGRGRDIIHPTKDAEQHRKFMEYEQNVIDEFKDLIPIIPYERKR
                     AANCGAGWKSIVISPFGEVRPCALFPKEFSLGNIFHDSYESIFNSPLVHKLWQAQAPRF
                     SEHCMKDKCPFSGYCGGCYLKGLNSNKYHRKNICSWAKNEQLEDVVQLI"
     CDS             4240..4401
                     /locus_tag="ctg7_6"
                     /transl_table=11
                     /translation="MSPAQRRILLYILSFIFVIGAVVYFVKSDYLFTLIFIAIAILFGM
                     RARKADSR"
     CDS             4398..5117
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 125.6; E-value:
                     4.3e-38)"
                     /gene_kind="transport"
                     /locus_tag="ctg7_7"
                     /transl_table=11
                     /translation="MSILDIHDVSVWYERDNVILEQVDLHLEKGAVYGLLGVNGAGKTT
                     LINTLTGVNRNFSGRFTLCGIEAEAGMPQKTSDQQKTHRYFAADYPLLFTEITAKDYVS
                     FVHSLYQKDFSEQQFASLAEAFHFSKYINRRISELSLGNRQKVVLMTGLLLRAPLFILD
                     EPLVGLDVESIEVFYQKTREYCEAGGTILFSSHLLDVVQRFCDYAAILHNKQIQKVIPI
                     GEETDLRREFFEVIGHE"
     CDS             5110..6420
                     /locus_tag="ctg7_8"
                     /transl_table=11
                     /translation="MNNIIPIMSLLFKQLYSRQGKKDAIRIAAGLVILAVFEIGLIRQA
                     GIDESVLRKTYIILALLLMNAYMVFLSVTSQWKESYMKLSCLLPISSRSFWLAQSVVLF
                     VDTCLRRTLFFFILPLFLFGNGTLSGAQTLFWLGRFSFFTVYSIIFGVVLSNHFVKKKN
                     LMFLLHAAIFACVCLSAALMPAATLPLCAVHMLWAVVIDFPVFLQAPPQQSRMHSFMRR
                     SEFSFYKREWNRFISSKAMLLNYAVMAAFSGFFSFQMMNTGIFNQQVIYIVISALLLIC
                     SPIALLYSIEKNDRMLLITLPIKGKTMFWAKYRFYSGLLAGEFLLVVMIVGFISGRPIS
                     VLTFLQCIELLLAGAFIRLTADEKRPFFSWQTEQQLWSGFSKYRSYLFCLPLFLATLAG
                     TAVSLAVIPIAGLVIVYYLQKQDGGFFDTSKRERLGS"
     CDS             6386..7570
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg7_9"
                     /sec_met_domain="Peptidase_M16_C (E-value: 8.7e-07,
                     bitscore: 21.2, seeds: 139, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIQAKENGLEVNLLKTHQFSTISIAASFLKPIESAAEPEEETIYF
                     YGAAAYLKEQIIDDFGCAAGSRFMYSANLFFDQQLKTCGTRLIHPLYNGNLHVDALMKT
                     FADLSFPSSLSFEAVEKARNELLLKIEKKFTDPFSYSAARLAEEVFGNPMYGTGMFGRK
                     DRIKAIHPKRFHDATDFIVDLVSQQKQLNILGQVQACDVRGHAPQTSAVTSERIPVNRH
                     VFETETRSAAGPSVLTLGFDCGEMKDASDYIKIQLIDGLLGKYGHSALFKHFREKDLAV
                     YHVITRYDVMNNLLLVSICTDQLHEIDIPPRVLEAVSAFHTDERELEQAKQFLRNELLL
                     QFDSPEGLLAYMGILRRFSCTKEALLDGISAVTCRDVLQFIATINYIGAHVVRG"
     CDS             7575..8855
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg7_10"
                     /sec_met_domain="Peptidase_M16 (E-value: 4.1e-15, bitscore:
                     48.2, seeds: 24, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKKAFFQQLDERTDIRYTDSGLKIFRLKFPRAHLRLCNVKIDFG
                     SRDVCIRAESGDTLLPYGTAHFLEHLLFWHNGRNLYSDFFAHGALLNAFTTYTDTNFMF
                     TSLPDRLRQTIPILLDALWNHSFDKKIVAQEKAVITSEIQTAHLNHQLSYHYQLISMLS
                     PSSPAAVFPAGRIEDIEALDISDLQKAYKAAYQTHRMTLFLIGGSENTETLLPPHLQLE
                     KRPDYHAERKIIPACPPALSQKMMLGDEERMEDTWTGLQIGALPGQNDLLTIKLYWDIA
                     ARILFQLDSPFFQEIQQTYRLEIDRLSAETYIYEDGGFLILHSQGTHSSAYIDVASYYV
                     TQKKEQAAAWLQYGKDSLTDAIIYDSDYVRKCFEWAAECDRCDCSFLDMYHIIQDMDAQ
                     VFLSLIDAMASSNKAIIHVSQKEAIRQ"
     CDS             8852..9553
                     /locus_tag="ctg7_11"
                     /transl_table=11
                     /translation="MKKNTVFTLLLLLIGMAAYSFGWVQAVAEAAAHYIQMINNDAVRL
                     GLLACTAALLMLPAFLYLHYVTQSVKNMTAAFQKLTQSHQSCCDFQQHNLCSRYAEDVK
                     SLRDGYKNVRQTYVMAAVLCQVIIFGCMFEIVKAVPFRLHTPPALSMGLAMLLILYLLF
                     CMRTYLRQLFRHGSLFRKVFAGVLAAAGIWWMLSFTISELLFLIILAAIQQIRSFIYKR
                     FSYRSTASLDL"
     CDS             complement(9559..10935)
                     /locus_tag="ctg7_12"
                     /transl_table=11
                     /translation="MKKNDLSALQENCFCFCDEEISREAPFQVPIDIPKGFKVDSAEAS
                     AAVTWSTDNLSCVSEPCLIQTGPETEDIGVRYAVRVQGTIMLLVSVSPVRNQYGQGDGA
                     VSVIHTEEIDQVVYYAAQSDRCPDFSQMTVEDLLIVPPFYGSPLTVAGTLVLPPSPDPQ
                     SYVFTANTGDSTVSVIDADLNTVVKTILFSAVPTNLGVTFDKAFTYVLHGNTNLVSVID
                     NKTLTIINTITVGGGPRKIEFDPTDEYAYVMAAGSIYMIDTANQSVIDVIAIPGALDFA
                     LDPNGQYVYTANGSSWSVDKYDVNTGQLVESIIDSFEFPSLIATPYAGNFAYVLNGELW
                     PKGVTEISLSPLSRGGDFNRLFETLRTIVFSLDSTRAYFLEPYSEPFLINNLYVVNTAR
                     QRIIANVPLPGAFDPAVTPDKQYIYAAQPNDNAVTVYRTSDYTAVKVIPVGAGPSAIVM
                     "
     CDS             complement(10974..11459)
                     /locus_tag="ctg7_13"
                     /transl_table=11
                     /translation="MFAYVLNFGLVQKVDLSTGEVTGTIERELIVSSIETDSPERYAYV
                     LEQEFLFNYLTVIDLNTFTISNTQELEPDGQYRMFTSGTGVYLYDDFTGNLYSVSPNGA
                     GVIGNLPQSATDYAFTPNGDFLYATRFIEQSIIVYNTDDYSEETVISLGVSPGAITI"
     CDS             complement(11492..12325)
                     /locus_tag="ctg7_14"
                     /transl_table=11
                     /translation="MRKNKLSFKEKAGAEQEECLCFCEGEASREAMFQAPIELPEGFVV
                     DPAEAVANVTWNADSLSCVSDRCLIQTGPEPDEVGVQFAVRLQGTVTLLVSVSPVRNQY
                     GQGDGAVSVIHNAEIDQVVYYSDEPDDCPDISNITIENLVVVPPFYGSPLSVTGTIVLV
                     PEPEPVYAFTANPNDQSVSVIDTNTHTVVTTIALPYNPAGIEITPDKSAVFVLHPNNNV
                     ISVIDYDTLTVTATILLDQPPRLIRFIPNHEFAYVFTGTAIYVIGIDTLTVERSI"
     CDS             12555..13700
                     /locus_tag="ctg7_15"
                     /transl_table=11
                     /translation="MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEE
                     DQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPRLSDLLLEIDKKQARLTGLLEYYFN
                     FFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFSMDY
                     ARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAVSHFQKAYSMAEAEKQPQLMG
                     RTLYNIGLCKNSQSQYEDAITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKA
                     HEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAIQGFFDFLESKMLYADLEDFT
                     IDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV"
     CDS             13684..13803
                     /locus_tag="ctg7_16"
                     /transl_table=11
                     /translation="MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI"
     CDS             13901..14212
                     /locus_tag="ctg7_17"
                     /transl_table=11
                     /translation="MSLKSVRTHFTQWNRENDVTEFETSSATVEQAAETIGVSLSRIAK
                     SLSFRGEGDQVILIVAAGDAKIDNKKSRQTFGFKARMLSPNEVLEQTGHEIGGVCPIG"
ORIGIN
        1 tctcctcaca gccgcatgcc agaacatgaa gaaaattgcc acatacctag ccaagcaggg
       61 ctaggcatgt gggagcgctt tttctgcatc tgaaacctga tgaatatcta aagaaactgt
      121 ataaaacaaa aaagcttgta gaaaaaacgt cgttttctct acaagctaag gacaaagccc
      181 gaagactttg tccttttgat cgtgattaca ttttttccgg agccgatacg ccgatcagct
      241 gaagcgcatt gttcagcgta atttgcgttg ccttcattaa agccaggcgc gcacggcttt
      301 tctcttcatt ttcagggtcg atgactttct ctgcgttgta gaagctgtgc agagcagagg
      361 ccagatcata aatatagttt gtgacacggt gcggaattcg tttttccgct gcttccgcca
      421 ctgcctctgg gaagccgccg atcgttttca gcagatcgta ttctttttct gattgaatat
      481 ggctgaaatc aagatccgcc accggtttaa gtccctgctc ttccccttgg cgaagcatgc
      541 tgcagattcg ggcatgtgca tattgtgcat aatacacagg gttttcgtta gatgttgata
      601 cagcaagatc taagtcaaaa tccatatggg tgtccgcact tcgcatcgca aagaagtaac
      661 gaacggcatc caagccgact tcttcaatca ggtcacgcat cgttacggct ttaccggtcc
      721 gtttgctcat tttcattttc tcgccgtttt tgtaaaggtg aacgagctga atgatttcta
      781 cttcaagcgt tcctttttca tagcccagcg cttcgattgc cgctttcata cgcgggatgt
      841 agccgtggtg atcggcgccc caaacgttga tcagcttatc aaagccgcgg tcaagcttgt
      901 ccttatgata cgcgatatca ggaagcaggt acgtgtacgt gccgtccttc ttgatcaata
      961 cgcggtcttt atcatcgcca aacgtcgtgg aacggaacca agtcgcgccg tcttcttcat
     1021 agacatggcc tttttcgcgg agcgcttcaa gcgccttgtc gatttttcca ttttggtaca
     1081 gtgaagtttc tgaataccac acatcaaacg gcacacggaa gttttccaag tccttgcgaa
     1141 gcttttccag ctcatatttc aggccgtatt cacggaaaaa cgcgaggcgt tcactttctt
     1201 cttcgttaac gaaacgatca ccgtattcct cagcgagacg ctttccgatc gcaatgatat
     1261 cctcgccgcg gtagccatcc tccggcatcg gtttttccaa tccgagtgct tcaaagtaac
     1321 ggacttcaac agaaagcgcc agattgttga tttggttacc cgcatcgtta atgtagtatt
     1381 cgcggcttac atcgtaaccc gcttttgaaa gcacgttgca caaggaatcg ccgacagccg
     1441 ccccgcgcgc atgcccgagg tgaaggtctc ccgtcggatt cgctgatacg aattctacct
     1501 gaactcgttc accgtttccg atgttcgttt ccccgtatgc ttcccctgct tccagtacag
     1561 acggaatcag ctttgttaaa tattgattgt tcatatagaa attgataaaa cccgggccgg
     1621 cgatatccag tttttcaatc gaagcctttc ctttatcaaa atgggcgaca atctcttcag
     1681 cgatttggcg cggcgctttt tttgccactc ttgccagctg catcgccata ttcgttgagt
     1741 agtcgccgtg tgttttatct ttcggtgttt ctaaaacaac attgggaatc tggctttctt
     1801 cagccagtcc cgctttcaga acggccgctt tgatttcttc tttcagcacg tccttcattt
     1861 gttccgcaat gttcatgcgt gagttcctcc ctcatacgtg atcgtcatat tatgtaaatg
     1921 ctcctgctcg tctccgacat gcatgtcata tgcgatgctg atacggcctt tttcttcatc
     1981 gagatcagat tgaatcgatt ttgtgcttgt tttcagctca agctcaccaa atgacatttt
     2041 atatttagca attgtggaag ctccctttac gaaacgctga ttcattttga cagcgcctga
     2101 cctcatgacc aggacttcgc cttcgcttac tttcacaatc gttttgactt tgcctaagtc
     2161 gtgttcttca taataagata gatacacttt attttgtttt acatagtaaa atccggtggt
     2221 gcggaattcg atgacttctt ggtttccgtc atcctcgatc acagacttca catggagtgt
     2281 tatcggtgtc tcttgcttca ttctgacacg tccttttcat cattccactt tgacttgaat
     2341 agtataatca ttcggtttgt aaacttcaac tgcttctatc ttaccatcat tgctcatcag
     2401 atttgaagat aaacctcata aaaagcattt ccttatatag aagagaaaat catatcacta
     2461 attaccttta gtaaatgtta catttttccg aaatctatca tttccttttc acattttttt
     2521 caaaatatat gtattgaatt agtaatttga tagttttaag ataaaagtac aacatagatc
     2581 tgttagaaaa acaaaaaaaa agggaggatt caattatgaa aaaagctgtc attgtagaaa
     2641 acaaaggttg tgcaacatgc tcgatcggag ccgcttgtct agtggacggt cctatccctg
     2701 attttgaaat tgccggtgca acaggtctat tcggtctatg ggggtaagga tctcccaaaa
     2761 gggcatagtc attctactat gcccttttta agtaaattac aatttttaac taaaatctac
     2821 ctcttggata tacatacaca accgtttttc atttgaatca tatgtatagg ggatggtatt
     2881 ttgtttatag agcagatgtt tccatttatt aatgaaagtg taagagttca ccagcttcct
     2941 gagggcggcg tgttagaaat cgactacttg cgcgataatg tctccatttc tgactttgag
     3001 tatttggatc tcaacaaaac agcttacgag ctctgcatgc gcatggatgg ccaaaaaaca
     3061 gctgagcaga ttttagctga gcaatgtgca gtgtatgatg aatcaccgga agaccataaa
     3121 gattggtatt acgacatgct caacatgctc cagaacaagc aggttatcca gcttggaaac
     3181 cgggccatcc gccataccat caccacgagc ggaagcaatg aatttccgat gcccctgcac
     3241 gccacctttg aactgacgca ccgctgtaat ttgaaatgcg cccactgtta tttggaaagc
     3301 tcgcctgaag cgctcggcac cgtgtcgatt gagcaattca aaaaaacggc tgatatgctg
     3361 tttgataacg gtgtattgac atgcgaaatc acaggcggag aaatttttgt ccatccaaac
     3421 gccaatgaga ttcttgacta tgtgtgtaaa aagttcaaaa aagtcgctgt cttaacaaac
     3481 ggaacactca tgcgaaaaga gagcctggag cttttgaaga cttacaagca aaaaatcatc
     3541 gtcggcattt ctcttgatag tgtcaattcc gaggtccatg actcctttag agggagaaaa
     3601 ggctcttttg cccaaacttg taaaacgata aaattgttga gtgaccacgg tatatttgtc
     3661 agagtcgcca tgtctgtctt cgaaaaaaac atgtgggaaa tccacgatat ggcccaaaag
     3721 gttcgggatc tcggagcgaa ggcgttttct tacaattggg ttgacgattt cggaagaggc
     3781 agggatatta tccatccaac gaaagacgcc gagcagcacc gcaagtttat ggaatacgag
     3841 caaaatgtga ttgatgagtt taaagatctg attccgatta ttccctatga gagaaaacgc
     3901 gcggcaaatt gcggcgctgg ctggaagtcc attgtgatca gcccgttcgg cgaagtacgt
     3961 ccttgcgccc tctttccaaa ggaattttca ttgggaaata tttttcatga ttcctatgaa
     4021 agcatcttta actcccctct cgtccataaa ctgtggcaag cgcaggcgcc gcggttcagc
     4081 gaacattgca tgaaagacaa atgcccgttc agcggctatt gcggaggctg ttacttaaaa
     4141 gggctgaact ctaacaaata tcaccgaaaa aacatttgct cttgggcgaa aaatgaacaa
     4201 ttagaagacg tggtccagct tatttaggag ggaaacgatt tgtcaccagc acaaagaaga
     4261 attttactgt atatcctttc atttatcttt gtcatcggcg cagtcgtcta ttttgtcaaa
     4321 agcgattatc tgtttacgct gattttcatt gccattgcca ttctgttcgg aatgcgcgcg
     4381 cggaaggctg actcgcgatg agcattttgg atatacacga tgtatccgtt tggtatgaac
     4441 gggacaacgt catcttagaa caagtggatt tacatttaga aaaaggcgcc gtttacgggc
     4501 tgcttggggt aaacggcgcc ggaaaaacga cactgattaa tacgctgaca ggcgtgaacc
     4561 gcaatttcag cgggcgcttt acactgtgcg gaattgaagc ggaggccggc atgccgcaga
     4621 aaacatcaga tcagcagaag actcaccgtt acttcgccgc cgattatccg ctgctattta
     4681 cagaaattac ggcgaaggac tatgtgtctt tcgtccattc gctttatcaa aaggattttt
     4741 cagagcagca gtttgccagt ttggccgagg cctttcattt ttcgaaatac atcaacagga
     4801 gaatttcgga gctgtccttg gggaacaggc aaaaggttgt attgatgaca ggcttattgc
     4861 tgcgcgctcc cctatttatt ttggatgagc cgctcgtcgg tttggatgtg gaatcgatag
     4921 aggtttttta tcagaaaacg cgcgagtact gtgaagcagg cgggaccatt ttgttttctt
     4981 ctcatctgct cgatgtcgtg caaagatttt gtgattacgc ggccattctt cacaacaaac
     5041 agatccaaaa ggtcattccg attggagagg agaccgatct gcggcgggaa tttttcgagg
     5101 tgatcggcca tgaataacat aatccccatc atgtctttgc tgttcaaaca gctttacagc
     5161 cggcaaggga aaaaggacgc catccgcatt gccgcaggcc ttgtcattct ggccgtattt
     5221 gaaatcgggc tgatccgcca ggccggcatt gatgaatcgg tgttgcgcaa aacgtatatc
     5281 atactcgcgc ttctcttaat gaacgcatat atggtgtttc tttctgtgac atctcaatgg
     5341 aaggaatctt atatgaaact gagctgcctg ctgccgattt cttcacggag cttttggctc
     5401 gcccagagtg tcgttttgtt tgtcgatacc tgtttgagaa gaacgttatt cttttttatt
     5461 ttaccgctgt tcttatttgg aaacggaacg ctgtcagggg cgcaaacatt gttttggctc
     5521 ggcaggtttt cgttttttac cgtttactcc attattttcg gagttgtgct aagcaaccac
     5581 ttcgtcaaaa agaagaactt gatgtttctg ctgcatgcgg cgatattcgc ctgtgtatgt
     5641 ctcagcgccg ctttgatgcc ggccgccacg cttccgcttt gcgcggtgca tatgctgtgg
     5701 gcggtggtca ttgactttcc tgtctttctg caggcgcctc cgcagcagag caggatgcat
     5761 tcatttatgc ggcgatctga gttttcgttt tacaaaagag aatggaaccg atttatctct
     5821 tctaaagcga tgctgttaaa ttacgcggta atggcggcgt tcagcggctt cttttcgttc
     5881 cagatgatga ataccggcat cttcaatcag caagtgattt atatcgtgat ttccgcgctt
     5941 ctgctcatct gctcgccgat cgcccttttg tactcgattg aaaaaaatga ccggatgctg
     6001 ctcatcacgc ttccgatcaa ggggaaaacg atgttttggg cgaaatatcg cttttattca
     6061 ggcctattgg caggcgaatt tctccttgtc gtgatgattg tgggtttcat cagcggccgt
     6121 ccgatttcag ttctcacttt tttgcagtgc attgagttgc tgctggcggg agcttttatc
     6181 aggttaactg cggatgaaaa aaggcccttc ttcagctggc agacggagca gcagctatgg
     6241 agcggtttct caaaatatcg gtcttatctg ttttgtcttc ccctattctt agccacatta
     6301 gccggcaccg ctgtatcctt ggcggtcatt ccgatagccg gtctggtcat agtctattac
     6361 ctgcaaaaac aggatggtgg tttctttgat acaagcaaaa gagaacggct tggaagttaa
     6421 cctgctgaag actcatcaat tttcaactat atcaatcgcc gcgagctttc tgaaaccgat
     6481 cgaaagcgcg gcggaacctg aggaagaaac gatttatttt tacggcgccg ccgcatacct
     6541 gaaggagcaa atcattgacg atttcggttg tgctgccggc agccgtttca tgtactcagc
     6601 gaatctcttt tttgatcagc agctgaaaac ctgcgggacc cgtttgattc acccattata
     6661 taatgggaat cttcatgttg acgccctgat gaaaactttt gcagacttgt ctttcccttc
     6721 ttctctttca ttcgaagcag ttgaaaaggc caggaatgag ctgctgctga aaatcgaaaa
     6781 gaaattcact gatcccttct cctactcggc ggcgcgcttg gctgaggagg tcttcggaaa
     6841 cccgatgtac ggcacaggca tgtttggccg gaaagacagg ataaaggcga ttcatcctaa
     6901 acggtttcac gacgcaacgg actttatcgt tgatttagtg tcccagcaaa aacagctgaa
     6961 catattggga caagtacaag catgtgatgt tcggggacat gccccgcaaa cgtccgctgt
     7021 aacgtccgaa agaatacccg taaaccggca tgtttttgaa acagagacac gcagcgctgc
     7081 aggtccttcc gtcttgacac tcggatttga ctgcggagag atgaaagatg ccagcgatta
     7141 tatcaaaata cagctaatag acggattact gggaaagtac ggccattccg ctttattcaa
     7201 gcacttcaga gagaaagatc tggcagtcta tcacgttatc acccgttacg atgtcatgaa
     7261 caatttgctg cttgtttcaa tatgtacaga tcagcttcat gagatagaca ttcccccgcg
     7321 cgttttggaa gctgtctcag cttttcatac agatgagcgg gaactagaac aggcaaagca
     7381 gtttttacgc aatgaattgc tgctgcaatt cgattctccc gaaggtctat tggcctatat
     7441 ggggatcctg cgccgcttct cctgcacgaa agaagccttg ctggatggca tctcagcggt
     7501 tacatgccgt gatgtattgc aattcatagc gaccatcaat tatatcggag ctcatgtagt
     7561 aaggggatga aaagatggaa aagaaagcct tttttcaaca gctggatgaa cgaaccgata
     7621 tcagatacac agacagcgga ctaaaaatat tcagattgaa attcccccgc gcccacctgc
     7681 ggctgtgcaa tgtaaaaatc gattttggaa gccgggatgt atgtatacgg gcagaatccg
     7741 gtgatacact gcttccgtat ggcacagcgc attttttaga gcatcttctc ttctggcata
     7801 acggccgcaa tctgtacagt gatttttttg cgcatggcgc gcttttaaac gcattcacta
     7861 cctacaccga taccaatttc atgtttacct cattaccgga tcggctcaga caaacgatcc
     7921 ccatcctgct tgatgcttta tggaaccact catttgacaa gaagattgtc gctcaggaaa
     7981 aagctgtgat tacaagcgaa attcaaacag cacaccttaa ccatcagctc tcatatcatt
     8041 atcagctgat cagcatgctt tctccctcct caccagctgc cgttttcccc gcaggcagaa
     8101 tcgaagatat tgaggcgctt gacatcagtg atttacaaaa agcctataag gctgcctacc
     8161 aaacccatcg gatgacgctg tttttgattg ggggctcaga aaatacagaa acgctattgc
     8221 ctcctcacct ccagctggaa aagcgtccgg attatcatgc tgaaagaaaa atcatcccgg
     8281 catgccctcc agccctctca caaaaaatga tgctgggtga tgaggagcgg atggaagata
     8341 catggaccgg attacagatc ggcgcacttc ccgggcaaaa cgatctgttg acgatcaaac
     8401 tttattggga tatcgcggct cgtattctat ttcaattaga ttcaccgttt tttcaagaga
     8461 ttcagcaaac gtaccgcctg gaaatcgatc gtttgtcagc cgagacttat atctatgagg
     8521 atggcggttt cttgatcctt cacagtcaag ggacccattc ttcagcttat atcgatgtgg
     8581 cttcatacta tgtcacacag aagaaagaac aggctgccgc ctggcttcaa tacggcaagg
     8641 actctttgac agacgcgatc atttatgaca gcgattatgt gcgtaaatgc ttcgagtggg
     8701 cagccgagtg tgacaggtgc gattgctcgt ttcttgacat gtaccacatc attcaagata
     8761 tggatgcgca ggttttcttg tctctcattg atgcaatggc atcttctaac aaagcgatca
     8821 ttcacgtttc tcagaaagag gcgatcagac aatgaaaaaa aacactgtct ttaccctttt
     8881 attgctgctt ataggtatgg cggcttattc cttcggctgg gttcaggccg ttgccgaagc
     8941 cgccgcccac tatatacaga tgatcaacaa tgatgctgta cggctcggac tgctggcttg
     9001 taccgctgcc ctcctgatgc tgcctgcttt tctctatctt cactatgtca cccaatctgt
     9061 gaaaaacatg acagcagctt ttcagaaact cacacagtct catcaaagct gctgtgactt
     9121 tcagcaacac aacttgtgca gccgatatgc agaagacgtc aaatcgctgc gtgacggcta
     9181 taaaaacgtc cgccaaacat acgtgatggc tgccgtgttg tgccaagtca tcatattcgg
     9241 ctgcatgttc gaaatcgtta aagccgtccc tttccgcctt cacacgcccc ctgccctttc
     9301 aatgggactt gcgatgctac tgatcctgta tttattgttt tgcatgcgaa cgtatttgcg
     9361 gcagcttttc cggcacggca gcctgttcag aaaagtgttt gcgggagtgc tggcagcagc
     9421 cgggatttgg tggatgctca gtttcactat aagtgaactg ctgtttctga ttatcctggc
     9481 tgccatccag caaatcagat catttattta taagcgattc tcttatcgca gcaccgcttc
     9541 tcttgatcta taaaatagct acatcacaat cgcagacggg cccgcgccaa ccggaatcac
     9601 cttcacggct gtataatcac ttgtgcggta cacagtcaca gcattgtcgt tgggctgtgc
     9661 cgcataaata tactgtttgt ccggcgttac ggcggggtca aaagcgccgg ggagaggcac
     9721 gtttgcaatg atcctctgtc tggccgtatt aactacgtat aagttattaa tgagaaacgg
     9781 ttcactatat ggctctagaa aataagctct agtactatcg agagaaaata caatcgtcct
     9841 cagcgtttca aataaacggt taaagtcccc tcccctgctt aatggtgaca aactaatctc
     9901 cgttacacct ttcggccaca gctcaccatt caacacgtac gcaaaatttc cagcgtaagg
     9961 agttgcaatt aaactgggaa attcaaatga atctatgata ctttctacca actgcccagt
    10021 atttacatca tatttatcaa ccgaccaact gcttccatta gcggtataga catactgtcc
    10081 attcggatca agagcaaagt ctaaagcgcc gggtatcgcg attacatcaa ttactgattg
    10141 attggccgtg tcaatcatat atatagatcc ggcggccatc acgtaggcgt actcatctgt
    10201 tggatcaaat tcgatctttc tcggtcctcc tccaacagta atggtattta tgatggttaa
    10261 tgttttatta tcaatcactg agacaaggtt ggtattccca tggaggacat acgtgaatgc
    10321 tttatcgaaa gtaacgccta gattagttgg cacagcagaa aacaggattg tttttacaac
    10381 agtgtttaaa tctgcatcaa tgaccgaaac cgtgctgtct ccagtattgg cagtaaacac
    10441 atacgattga gggtctggcg atggcggcaa aacaagtgtc ccggccaccg tgagcggact
    10501 gccgtagaaa ggcggcacga tgagaagatc ttccactgtc atctgactaa aatcaggaca
    10561 gcggtcagac tgtgctgcat aataaacaac ttgatcaatc tcttcagtat gaatcacaga
    10621 aaccgccccg tctccttgtc catattgatt tcgcacaggc gacacactga caagaagcat
    10681 aatggtccct tgtactctca cagcatatcg aacaccaata tcttctgttt ccggcccagt
    10741 ctggattaag caaggctcac tgacacagga caaattgtct gtactccatg taacagccgc
    10801 tgacgcttcg gctgaatcaa ccttgaagcc cttagggata tctattggca cttgaaacgg
    10861 cgcttcccgg ctaatttcct catcacaaaa acagaaacaa ttttcctgta aagcagaaag
    10921 atcattcttt ttcatgaatt cacgtccttt taaaaatgca caacatgcag cggttaaatc
    10981 gtaatggcac ccggtgaaac cccaagagat atcacagttt cctcagaata atcatctgtg
    11041 ttgtaaacaa tgatgctctg ctctataaaa cgagttgcat acagaaaatc gccattcggg
    11101 gtaaacgcat agtctgttgc tgattgcgga agatttccaa ttacacctgc tccattcgga
    11161 ctgacagaat ataaattgcc ggtaaagtcg tcatataaat acacacctgt tccgctcgta
    11221 aacattctat attggccgtc aggctccagt tcttgggtat tgctgatcgt aaacgtattt
    11281 aagtcaataa ccgtcaaata attgaataag aattcttgtt ctaagacata cgcataccgc
    11341 tccggagaat ctgtttctat ggatgatacg ataagctctc gttcaatcgt tcctgtgact
    11401 tcaccggtgg ataagtccac tttttgcact aacccaaaat taagaacata ggcgaacaac
    11461 ccattggggt caattgcaat atcaaaacct tctatataga cctctcaaca gtcaacgtat
    11521 caattccaat cacataaatg gcagtaccgg tgaaaacata agcaaactca tgattaggga
    11581 taaatctaat caatcgaggc ggctgatcca gcaatatagt tgctgtcact gttaatgtgt
    11641 cataatcgat gacagaaatc acattattgt tgggatgtaa gacaaacaca gcgcttttat
    11701 ctggcgtaat ttcaatacct gccggattgt acggaagagc aatcgttgtc acaacagtat
    11761 gggtgtttgt atcaatcaca gaaactgatt gatcatttgg attcgctgta aacgcgtaga
    11821 cgggttccgg ctccggtaca agcacaatcg tccccgtcac cgataaagga ctgccgtaaa
    11881 acggcggaac aacaacaagg ttctcaatcg tgatgttact gatatccggg caatcatccg
    11941 gttcatcgga ataatagacg acttgatcga tttctgcatt gtgaatgacg gaaacagccc
    12001 catccccttg tccatattga tttcgcacag gtgagacact gacgagcagc gtaacggttc
    12061 cctgcagacg cactgcaaat tgaacaccga cctcatcagg ctctggcccc gtttgaatta
    12121 agcaacggtc actgacacaa gaaaggctgt ctgcattcca cgtcacgttt gcaacagctt
    12181 cagccggatc aacgacaaat ccttcaggta attcaatcgg cgcttgaaac atggcttctc
    12241 ggcttgcttc tccctcacaa aaacataagc actcttcttg ttctgctcct gctttttctt
    12301 taaaggaaag cttgtttttt ctcattcctt cacttccttt tcagacatac actgctcacg
    12361 cagatataac agaatatgga agagtaacgt gtttgcttgt acaaattact gagggtaaaa
    12421 gcttcttttt gaaaatatat ctccaaacat gcggtgtaac gaaagaaaaa attgcggttt
    12481 ttttgcgctt ctcacaagaa tgttgatgat aaaatatgac atatatagaa ttatgaaggg
    12541 agggatttgc aaacgtgaca ggtgtcatat cttcttcttc catcggagaa aagattaacg
    12601 aatggtatat gtacatacgc cgattcagca tacccgatgc agaatatttg cgacgagaaa
    12661 tcaagcaaga gctggatcaa atggaagaag atcaagacct tcatttgtac tattcactga
    12721 tggagtttcg gcacaacctg atgcttgagt accttgaacc gttagaaaaa atgaggattg
    12781 aggaacagcc gagactgtct gatctgctgc ttgagattga taaaaaacag gctcgtttaa
    12841 caggtctgct tgagtactat tttaatttct tcagaggcat gtatgagctg gatcagcggg
    12901 aatatctgtc ggctattaaa tttttcaaaa aggccgaaag caagctgata ttcgttaagg
    12961 atcggataga gaaagctgag tttttcttta agatgtctga atcttattac tatatgaaac
    13021 aaacgtattt ttcaatggac tatgcacggc aagcatatga aatatacaaa gaaaatgaag
    13081 cttataatat aagattgctg cagtgtcatt ctttatttgc caccaatttt ttagatttaa
    13141 aacagtatga ggatgccgtc tcacattttc aaaaagctta ttctatggca gaagctgaaa
    13201 agcagcccca attaatggga agaactttgt acaatatcgg gctttgtaaa aacagccaaa
    13261 gccaatatga ggatgccata acatatttca aaagagcaat agctgttttt gaagaatcaa
    13321 atattcttcc ttccttacct caagcgtatt ttttaattac acagatccat tataaattag
    13381 gaaaaatgga taaagctcat gaatatcata gtaagggaat ggcttattca caaaaggccg
    13441 gagatgtaat atatttatca gagtttgaat ttttgaaatc tttatactta tcaggcccgg
    13501 atgaagaagc aattcaagga ttttttgatt ttctcgaaag taaaatgttg tatgctgatc
    13561 ttgaagattt cactattgat gtggcaaaat attatcatga acgtaaaaat tttcaaaaag
    13621 cttctgctta ttttttgaag gtggaacaag taaggcaact tattcaagga ggagtgagtt
    13681 tgtatgaaat tgaagtctaa actattactc tcttgtctgg ctctaagcac tgtgttcgtg
    13741 gcaacaacta ttgcaaatgc acctacacac caaattgaag ttgcacaacg aggaatgatt
    13801 taaccgccgt ccatcggcgg tttttttgtc ccctctttac ccagtctccc aatccattct
    13861 atgatttttt caataatctt aaaaatagtg gaaatgcagc atgtctctaa aaagtgtgag
    13921 aacccacttt actcaatgga atcgagaaaa tgatgtgacg gagttcgaaa cgtcgagtgc
    13981 gacagttgaa caggcagctg agacaatcgg cgtaagcctg tctagaatcg ccaagtccct
    14041 gtccttcaga ggggaaggag atcaggtgat tctgattgtg gcagccggcg atgccaagat
    14101 cgacaacaaa aagtccaggc aaacatttgg ctttaaagca agaatgctct ctcctaatga
    14161 ggtactggag cagacaggcc atgaaattgg aggagtttgc ccaattggat agactcatga
    14221 tcctgag
//
