LOCUS gnlProkkaGAMLCGEA_34 14291 bp DNA linear UNK 01-JAN-1980 DEFINITION gnl|Prokka|GAMLCGEA_34. ACCESSION gnlProkkaGAMLCGEA_34 VERSION gnlProkkaGAMLCGEA_34 KEYWORDS . SOURCE ORGANISM . COMMENT ##antiSMASH-Data-START## Version :: 7.1.0 Run date :: 2025-04-28 19:36:14 Original ID :: gnl|Prokka|GAMLCGEA_34 NOTE: This is a single region extracted from a larger record! Orig. start :: 0 Orig. end :: 14291 ##antiSMASH-Data-END## FEATURES Location/Qualifiers protocluster 1..14291 /aStool="rule-based-clusters" /category="RiPP" /contig_edge="True" /core_location="[2679:4291]" /cutoff="20000" /detection_rule="(subtilosin or thuricin or TIGR04404 or Subtilosin_A or skfc)" /neighbourhood="10000" /product="sactipeptide" /protocluster_number="1" /tool="antismash" proto_core 2680..4291 /aStool="rule-based-clusters" /tool="antismash" /cutoff="20000" /detection_rule="(subtilosin or thuricin or TIGR04404 or Subtilosin_A or skfc)" /neighbourhood="10000" /product="sactipeptide" /protocluster_number="1" cand_cluster 1..14291 /candidate_cluster_number="1" /contig_edge="True" /detection_rules="(subtilosin or thuricin or TIGR04404 or Subtilosin_A or skfc)" /kind="single" /product="sactipeptide" /protoclusters="1" /tool="antismash" region 1..14291 /candidate_cluster_numbers="1" /contig_edge="True" /product="sactipeptide" /region_number="1" /rules="(subtilosin or thuricin or TIGR04404 or Subtilosin_A or skfc)" /tool="antismash" CDS 133..306 /locus_tag="ctg34_1" /transl_table=11 /translation="MGALFLHLKPDEYLKKLYKTKKLVEKTSFSLQAKDKVRRLCPFVR DYIFSGADTPIS" CDS complement(270..1940) /locus_tag="ctg34_2" /transl_table=11 /translation="MNIAEQMKDVLKEEIKAAVLKAGLAEESQIPNVVLEIPKDKTHGD YSTNMAMQLARVAKKAPRQIAEEIVAHFDKGKASIEKLDIAGPGFINFYMNNQYLTKLI PSVLEAGEAYGETNIGNGERVQVEFVSANPTGDLHLGHARGAAVGDSLCNVLSKAGYDV SREYYINDAGNQINNLALSVEVRYFEALGLEKPMPEDGYRGEDIIAIGKRLAEEYGDRF VNEEESERLAFFREYGLKYELEKLRKDLENFRVPFDVWYSETSLYQNGKIDKALEALRG KGHVYEEDGATWFRSTTFGDDKDRVLIKKDGTYTYLLPDIAYHKDKLDRGFDKLINVWG ADHHGYIPRMKAAIEALGYEKGTLEVEIIQLVHLYKNGEKMKMSKRTGKAVTMRDLIEE VGLDAVRYFFAMRSADTHMDFDLDLAVSTSNENPVYYAQYAHARICSMLRQGEEQGLKP AADLDFSHIQSEKEYDLLKTIGGFPEAVAEAAEKRIPHRVTNYIYDLASALHSFYNAEK VIDPENEEKSRARLALMKATQITLNNALQLIGVSAPEKM" CDS complement(1937..2365) /locus_tag="ctg34_3" /transl_table=11 /translation="MKQETPITLHVKSVIEDDGNQEVIEFRTTGFYYVKQNKVYLSYYE EHDLGKVKTIVKVSEGEVLVMRSGAVKMNQRFVKGASTIAKYKMSFGELELKTSTKSIQ SDLDEEKGRISIAYDMHVGDEQEHLHNMTITYEGGTHA" CDS 2680..2811 /gene_functions="biosynthetic (rule-based-clusters) sactipeptide: Subtilosin_A" /gene_functions="biosynthetic-additional (sactipeptides) predicted sactipeptide" /gene_kind="biosynthetic" /locus_tag="ctg34_4" /sec_met_domain="Subtilosin_A (E-value: 2.2e-30, bitscore: 97.4, seeds: 2, tool: rule-based-clusters)" /transl_table=11 /translation="MKKAVIVENKGCATCSIGAACLVDGPIPDFEIAGATGLFGLWG" CDS_motif 2680..2709 /prepeptide="leader" /note="peptide class: {self.peptide_class}" /note="predicted sequence: MKKAVIVENK" /locus_tag="ctg34_4_sactipeptide" /aSTool="sactipeptides" /tool="antismash" CDS_motif 2710..2811 /prepeptide="core" /locus_tag="ctg34_4_sactipeptide" /peptide="sactipeptide" /predicted_class /score="33.00" /molecular_weight="0.0" /monoisotopic_mass="0.0" /core_sequence="GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG" /aSTool="sactipeptides" /tool="antismash" /leader_sequence="MKKAVIVENK" /leader_location="[2679:2709](+)" CDS 2945..4291 /gene_functions="biosynthetic (rule-based-clusters) sactipeptide: subtilosin" /gene_functions="biosynthetic-additional (rule-based-clusters) Subtilosin_rSAM_RRE" /gene_functions="biosynthetic-additional (rule-based-clusters) PF05402" /gene_functions="biosynthetic-additional (rule-based-clusters) PF04055" /gene_functions="biosynthetic-additional (rule-based-clusters) TIGR04085" /gene_functions="biosynthetic-additional (rule-based-clusters) SPASM" /gene_kind="biosynthetic" /locus_tag="ctg34_5" /sec_met_domain="subtilosin (E-value: 0.0, bitscore: 1081.4, seeds: 6, tool: rule-based-clusters)" /sec_met_domain="Subtilosin_rSAM_RRE (E-value: 1.2e-30, bitscore: 98.4, seeds: 8, tool: rule-based-clusters)" /sec_met_domain="PF05402 (E-value: 8.1e-09, bitscore: 28.4, seeds: 461, tool: rule-based-clusters)" /sec_met_domain="PF04055 (E-value: 2.6e-23, bitscore: 75.9, seeds: 518, tool: rule-based-clusters)" /sec_met_domain="TIGR04085 (E-value: 1.3e-11, bitscore: 37.5, seeds: 281, tool: rule-based-clusters)" /sec_met_domain="SPASM (E-value: 4e-11, bitscore: 36.0, seeds: 166, tool: rule-based-clusters)" /transl_table=11 /translation="MFIEQMFPFINESVRVHQLPEGGVLEIDYLRDNVSISDFEYLDLN KTAYELCMRMDGQKTAEQILAEQCAVYDESPEDHKDWYYDMLNMLQNKQVIQLGNRAIR HTITTSGSNEFPMPLHATFELTHRCNLKCAHCYLESSPEALGTVSIEQFKKTADMLFDN GVLTCEITGGEIFVHPNANEILDYVCKKFKKVAVLTNGTLMRKESLELLKTYKQKIIVG ISLDSVNSEVHDSFRGRKGSFAQTCKTIKLLSDHGIFVRVAMSVFEKNMWEIHDMAQKV RDLGAKAFSYNWVDDFGRGRDIIHPTKDAEQHRKFMEYEQNVIDEFKDLIPIIPYERKR AANCGAGWKSIVISPFGEVRPCALFPKEFSLGNIFHDSYESIFNSPLVHKLWQAQAPRF SEHCMKDKCPFSGYCGGCYLKGLNSNKYHRKNICSWAKNEQLEDVVQLI" CDS 4304..4465 /locus_tag="ctg34_6" /transl_table=11 /translation="MSPAQRRILLYILSFIFVIGAVVYFVKSDYLFTLIFIAIAILFGM RARKADSR" CDS 4462..5181 /gene_functions="transport (smcogs) SMCOG1000:ABC transporter ATP-binding protein (Score: 124.3; E-value: 1.1e-37)" /gene_kind="transport" /locus_tag="ctg34_7" /transl_table=11 /translation="MSILDIHDVSVWYERDNVILEQVDLHLEKGAVYGLLGVNGAGKTT LINTLTGVNRNFSGRFTLCGIEAEAGMPQKTSDQLKTHRYFAADYPLLFTEITAKDYVS FVHSLYQKDFSEQQFASLAEAFHFSKYINRRISELSLGNRQKVVLMTGLLLRAPLFILD EPLVGLDVESIEVFYQKTREYCEAGGTILFSSHLLDVVQRFCDYAAILHNKQIQKVIPI GEETDLRREFFEVIGHE" CDS 5174..6484 /locus_tag="ctg34_8" /transl_table=11 /translation="MNNIIPIMSLLFKQLYSRQGKKDAIRIAAGLVILAVFEIGLIRQA GIDESVLRKTYIILALLLMNAYMVFLSVTSQWKESYMKLSCLLPISSRSFWLAQSVVLF VDTCLRRTLFFFILPLFLFGNGTLSGAQTLFWLGRFSFFTVYSIIFGVVLSNHFVKKKN LMFLLHAAIFACVCLSAALMPAATLPLCAVHMLWAVVIDFPVFLQAPPQQSRMHSFMRR SEFSFYKREWNRFISSKAMLLNYAVMAAFSGFFSFQMMNTGIFNQQVIYIVISALLLIC SPIALLYSIEKNDRMLLITLPIKRKTMFWAKYRFYSGLLAGGFLLVVMIVGFISGRPIS VLTFLQCIELLLAGAFIRLTADKKRPFFSWQTEQQLWSGFSKYRSYLFCLPLFLATLAG TAVSLAVIPIAGLVIVYYLQKQDGGFFDTSKRERLGS" CDS 6450..7634 /gene_functions="biosynthetic-additional (rule-based-clusters) Peptidase_M16_C" /gene_kind="biosynthetic-additional" /locus_tag="ctg34_9" /sec_met_domain="Peptidase_M16_C (E-value: 1.4e-06, bitscore: 21.2, seeds: 139, tool: rule-based-clusters)" /transl_table=11 /translation="MIQAKENGLEVNLLKTHQFSTISIAASFLKPIESAAEPEEETIYF YGAAAYLKEQIIDDFGCAAGSRFMYSANLFFDQQLKTCGTRLIHPLYNGNLHVDALMKT FADLSFPSSLSFEAVEKARNELLLKIEKKFTDPFSYSAARLAEEVFGNPMYGTGMFGRK DRIKAIHPKRFHDATDFIVDLASQQKQLNILGQVQACDVRGHAPQTSAVTSERIPVNRH VFETETRSAAGPSVLTLGFDCGEMKDASDYIKIQLIDGLLGKYGHSALFKHFREKDLAV YHVITRYDVMNNLLLVSICTDQLHEIDIPPRVLEAVSAFHTDERELEQAKQFLRNELLL QFDSPEGLLAYMGILRRFSCTKEALLDGISAVTCRDVLQFIATINYIGAHVVRG" CDS 7639..8919 /gene_functions="biosynthetic-additional (rule-based-clusters) Peptidase_M16" /gene_kind="biosynthetic-additional" /locus_tag="ctg34_10" /sec_met_domain="Peptidase_M16 (E-value: 6.1e-15, bitscore: 48.2, seeds: 24, tool: rule-based-clusters)" /transl_table=11 /translation="MEKKAFFQQLDERTDIRYTDSGLKIFRLKFPRAHLRLCNVKIDFG SRDVCIRAESGDTLLPYGTAHFLEHLLFWHNGRNLYSDFFAHGALLNAFTTYTDTNFMF TSLPDRLRQTIPILLDALWNHSFDKKIVAQEKAVITSEIQTAHLNHQLSYHYQLISMLS PSSPAAVFPAGRIEDIEALDISDLQKAYKAAYQTHRMTLFLIGGSENTETLLPPHLQLE KRPDYHTERKIIPACPPALSQKMMLGDEERMEDTWTGLQIGALPGQNDLLTIKLYWDIA ARILFQLDSPFFQEIQQTYRLEIDRLSAETYIYEDGGFLILHSQGTHSSAYIDVASYYV TQKKEQAAAWLQYGKDSLTDAIIYDSDYVRKCFEWAAECDRCDCSFLDMYHIIQDMDAQ VFLSLIDAMASSNKAIIYISQKEAIRQ" CDS 8916..9617 /locus_tag="ctg34_11" /transl_table=11 /translation="MKKNTVFTLLLLLIGMAAYSFGWVQAVAEAAAHYIQMINNDAVRL GLLACTAALLMLPAFLYLHYVTQSVKNMTAAFQKLTQSHQSCCDFQQHNLCSRYAEDVK SLRDGYKNVRQTYVMAAVLCQVIIFGCMFEIVKAVPFRLHTPPALSMGLAMLLILYLLF CMRTYLRQLFRHGSLFRKVFAGALAAAGIWWMLSFTISELLFLIILAAIQQIGSFIYKR FSYRSTASLDL" CDS complement(9623..10990) /locus_tag="ctg34_12" /transl_table=11 /translation="MDLSALQENCFCFCDEEISREAPFQVPIDIPEGFKVDSAEASAAV TWSTDNVSCISEPCLIQTGPETEDIGVRYAVRVQGTIMLLVSVSPVRNQYGQGDGAVSV IHTEEIDQVVYYAAQSDRCPDFSQMTVEDLLIVPPFYGSPLTVAGTLVLPPSPDPQSYV FTANTGDSTVSVIDADLNTVVKTILFSAVPTNLGVTFDKAFTYVLHGNTNLVSVIDNKT LTIINTITVGGGPRKIEFDPTDEYAYVMAAGSIYMIDTANQSVIDVIAIPGALDFALDP NGQYVYTANGSSWSVDKYDVNTGQLVESIIDTFEFPSLIATPYAGNFAYVLNGELWPKG VTEISLSPLSRGGDFSRLFETLRTIVFSLDSTRAYFLEPYSEPFLINNLYVVNTARQRI IANVSLPGAFDLAVTPDKQYIYAAQPNDNAVTVYRTSDYTAVKVISVGAGPSAIVM" CDS complement(11039..11485) /locus_tag="ctg34_13" /transl_table=11 /translation="MDLTTGEVTGTIERELIVSSIETDSTERYAYVLEQEFLFNYLTVI DLNTFTIRDTQELEPDGLYVMFTSGSEVYLHDSFTGNLYSVSPNGAGVIGNLPQSATDY AFTPNGDFLYATRFIEQSIIVYNTDDYSEETVISLGVSPGAITI" CDS complement(11557..12390) /locus_tag="ctg34_14" /transl_table=11 /translation="MRKNKLSFKEKAGAEQEECLCFCEGEASREAMFQAPIELPEGFVV DPAEAVANVTWNADSLSCVSDRCLIQTGPEPDEVGVQFAVRLQGTVTLLVSVSPVRNQY GQGDGAVSVIHNAEIDQVVYYSDEPDDCPDISNITIENLVVVPPFYGSPLSVTGTIVLV PEPEPVYAFTANPNDQSVSVIDTNTHTVVTTIALPYNPAGIEITPDKSAVFVLHPNNNV ISVIDYDTLTVTATILLDQPPRLIRFIPNHEFAYVFTGTAIYVIGIDTLTVERSI" CDS 12620..13765 /locus_tag="ctg34_15" /transl_table=11 /translation="MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEE DQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPRLSDLLLEIDKKQARLTGLLEYYFN FFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFSMDY ARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAVSHFQKAYSMAEAEKQPQLMG RTLYNIGLCKNSQSQYEDAITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKA HEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAIQGFFDFLESKMLYADLEDFT IDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV" CDS 13749..13868 /locus_tag="ctg34_16" /transl_table=11 /translation="MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI" CDS 13966..14277 /locus_tag="ctg34_17" /transl_table=11 /translation="MSLKSVRTHFTQWNRENDVTEFETSSATVEQAAETIGVSLSRIAK SLSFRGEGDQVILIVAAGDAKIDNKKSRQTFGFKARMLSPNEVLEQTGHEIGGVCPIG" ORIGIN 1 cacaacaccg gcatgggctt cgctattgca ggttgagggg acaacgaaat gtgagtgaac 61 aagttctcct cacagccgca tgccagaaca tgaagaagat tgccacatac ctagccaagc 121 agggctaggc atgtgggagc gctttttctg catctgaaac ctgatgaata tctaaagaaa 181 ctgtataaaa caaaaaagct tgtagaaaaa acgtcgtttt ctctacaagc taaggacaaa 241 gtccgaagac tttgtccttt tgttcgtgat tacatttttt ccggagccga tacgccgatc 301 agctgaagcg cattgttcag cgtaatttgc gttgccttca ttaaagccag gcgcgcgcgg 361 cttttctctt cattttcagg gtcgatgact ttctctgcgt tgtagaagct gtgcagagca 421 gaagccagat cataaatata gttagtgaca cggtgcggaa ttcgtttttc cgctgcttcc 481 gccactgcct ctgggaagcc gccgatcgtt ttcagcagat cgtattcttt ttctgattga 541 atatggctga aatcaagatc tgccgccggt ttaagcccct gctcttcccc ttggcgaagc 601 atgctgcaaa tacgtgcatg cgcgtattgt gcataataca cagggttttc gttagatgtt 661 gatacagcaa gatctaagtc aaaatccata tgggtgtctg cacttcgcat tgcaaagaag 721 taacgcacgg catccaagcc gacttcttca atcaggtcac gcatcgttac ggctttaccg 781 gtccgtttgc tcattttcat tttctcgccg tttttgtaaa ggtgaacgag ctgaatgatt 841 tctacttcaa gcgttccttt ttcatacccc agcgcttcga ttgccgcttt catacgcggg 901 atgtagccgt ggtgatcggc gccccaaacg ttgatcagct tatcaaagcc gcggtcaagc 961 ttgtccttat gatacgcgat atcaggaagc aggtacgtgt acgtgccgtc cttcttgatc 1021 aatacgcggt ctttatcatc gccaaacgtc gtggaacgga accaagtcgc gccgtcttct 1081 tcatagacat ggccttttcc gcggagcgct tcaagcgcct tgtcgatttt tccattttgg 1141 tacagtgaag tttctgaata ccacacatca aacggcacac ggaagttttc caagtccttg 1201 cgaagctttt ccagctcgta tttcaggccg tattcacgga aaaacgcgag gcgttcactt 1261 tcctcttcgt taacgaaacg gtcaccgtat tcctcagcga gacgctttcc gatcgcaatg 1321 atatcctcgc cgcggtagcc atcctccggc atcggttttt ccaatccgag tgcttcgaag 1381 taacggactt caacagagag cgccagattg ttgatttggt ttcccgcatc gttaatgtag 1441 tattcgcggc ttacatcgta acccgctttt gaaagcacgt tgcacaagga atcgccgaca 1501 gccgccccgc gcgcatgccc gaggtgaagg tctcccgtcg gattcgctga tacgaattct 1561 acctgaactc gttcaccgtt tccgatgttc gtttccccgt atgcttcccc tgcttccagt 1621 acagacggaa tcagctttgt taaatattga ttgttcatat agaaattgat aaaacccggg 1681 ccggcgatat ccagtttttc aatcgaagcc tttcctttat caaaatgggc gacaatctct 1741 tcagcgattt ggcgcggcgc tttttttgcc actcttgcca gctgcatcgc catattcgtt 1801 gagtagtcgc cgtgtgtttt atctttcggt atttctaaaa caacattggg aatctggctt 1861 tcttcagcca gtcccgcttt cagaacggcc gctttgattt cttctttcag cacgtccttc 1921 atttgttccg caatgttcat gcgtgagttc ctccctcata cgtgatcgtc atattatgta 1981 aatgctcctg ctcgtctccg acatgcatgt catatgcgat gctgatacgg cctttttctt 2041 catcgagatc agattgaatc gattttgtgc ttgttttcag ctcaagctca ccaaatgaca 2101 ttttatattt agcaattgtg gaagctccct ttacgaaacg ctgattcatt ttgacagcgc 2161 ctgacctcat gaccaggact tcgccttcgc ttactttcac aatcgttttg actttgccta 2221 agtcgtgttc ttcataataa gatagataca ctttattttg ttttacatag taaaatccgg 2281 tggtgcggaa ttcgatgact tcttggtttc cgtcatcctc gatcacagac ttcacatgga 2341 gtgttatcgg tgtctcttgc ttcattctga cacgtccttt tcatcattcc actttgactt 2401 gaatagtata atcattcggt ttgtaaactt caactgcttc tatcttacca tcattgctca 2461 tcagatttga agataaacct cataaaaagc atttccttat atagaagaga aaatcatatc 2521 actaattacc tttagtaaat gttacatttt tccgaaatct atcatttcct tttcacattt 2581 ttttcaaaat atatgtattg aattagtaat ttgatagttt taagataaaa gtacaacata 2641 gatctgttag aaaaacaaaa aaaaagggag gattcaatta tgaaaaaagc tgtcattgta 2701 gaaaacaaag gttgtgcaac atgctcgatc ggagccgctt gtctagtgga cggtcctatc 2761 cctgattttg aaattgccgg tgcaacaggt ctattcggtc tatgggggta aggatctccc 2821 aaaagggcat agtcattcta ctatgccctt tttaagtaaa ttacaatttt taactaaaat 2881 ctacctcttg gatatacata cacaaccgtt tttcatttga atcatatgta taggggatgg 2941 tattttgttt atagagcaga tgtttccatt tattaatgaa agtgtaagag ttcaccagct 3001 tcctgagggc ggcgtgttag aaatcgacta cttgcgcgat aatgtctcca tttctgactt 3061 tgagtatttg gatctcaaca aaacagctta cgagctctgc atgcgcatgg atggccaaaa 3121 aacagctgag cagattttag ctgagcaatg tgcagtgtat gatgaatcac cggaagacca 3181 taaagattgg tattacgaca tgctcaacat gctccagaac aagcaggtta tccagcttgg 3241 aaaccgggcc atccgccata ccatcaccac gagcggaagc aatgaatttc cgatgcccct 3301 gcacgccacc tttgaactga cgcaccgctg taatttgaaa tgcgcccact gttatttgga 3361 aagctcgcct gaagcgctcg gcaccgtgtc gattgagcaa ttcaaaaaaa cggctgatat 3421 gctgtttgat aacggtgtat tgacatgcga aatcacaggc ggagaaattt ttgtccatcc 3481 aaacgccaat gagattcttg actatgtgtg taaaaagttc aaaaaagtcg ctgtcttaac 3541 aaacggaaca ctcatgcgaa aagagagcct ggagcttttg aagacttaca agcaaaaaat 3601 catcgtcggc atttctcttg atagtgtcaa ttccgaggtc catgactcct ttagagggag 3661 aaaaggctct tttgcccaaa cttgtaaaac gataaaattg ttgagtgacc acggtatatt 3721 tgtcagagtc gccatgtctg tcttcgaaaa aaacatgtgg gaaatccacg atatggccca 3781 aaaggttcgg gatctcggag cgaaggcgtt ttcttacaat tgggttgacg atttcggaag 3841 aggcagggat attatccatc caacgaaaga cgccgagcag caccgcaagt ttatggaata 3901 cgagcaaaat gtgattgatg agtttaaaga tctgattccg attattccct atgagagaaa 3961 acgcgcggca aattgcggcg ctggctggaa gtccattgtg atcagcccgt tcggcgaagt 4021 acgtccttgc gccctctttc caaaggaatt ttcattggga aatatttttc atgattccta 4081 tgaaagcatc tttaactccc ctctcgtcca taaactgtgg caagcgcagg cgccgcggtt 4141 cagcgaacat tgcatgaaag acaaatgccc gttcagcggc tattgcggag gctgttactt 4201 aaaagggctg aactctaaca aatatcaccg aaaaaacatt tgctcttggg cgaaaaatga 4261 acaattagaa gacgtggtcc agcttattta ggagggaaac gatttgtcac cagcacaaag 4321 aagaatttta ctgtatatcc tttcatttat ctttgtcatc ggcgcagtcg tctattttgt 4381 caaaagcgat tatctgttta cgctgatttt cattgccatt gccattctgt tcggaatgcg 4441 cgcgcggaag gctgactcgc gatgagcatt ttggatatac acgatgtatc cgtttggtat 4501 gaacgggaca acgtcatctt agaacaagtg gatttacatt tagaaaaagg cgccgtttac 4561 gggctgcttg gggtaaacgg cgccggaaaa acgacactga ttaatacgct gacaggcgtg 4621 aaccgcaatt tcagcgggcg ctttacactg tgcggaattg aagcggaggc cggcatgccg 4681 cagaaaacat cagatcagct gaagactcac cgttacttcg ccgccgatta tccgctgcta 4741 tttacagaaa ttacggcgaa ggactatgtg tctttcgtcc attcgcttta tcaaaaggat 4801 ttttcagagc agcagtttgc cagtttggcc gaggcctttc atttttcgaa atacatcaac 4861 aggagaattt cggagctgtc cttggggaac aggcaaaagg ttgtattgat gacaggctta 4921 ttgctgcgcg ctcccctatt tattttggat gagccgctcg tcggtttgga tgtggaatcg 4981 atagaggttt tttatcagaa aacgcgcgag tactgtgaag caggcgggac cattttgttt 5041 tcttctcatc tgctcgatgt cgtgcaaaga ttttgtgatt acgcggccat tcttcacaac 5101 aaacagatcc aaaaggtcat tccgattgga gaggagaccg atctgcggcg ggaatttttc 5161 gaggtgatcg gccatgaata acataatccc catcatgtct ttgctgttca aacagcttta 5221 cagccggcaa gggaaaaagg acgccatccg cattgccgca ggccttgtca ttctggccgt 5281 atttgaaatc gggctgatcc gccaggccgg cattgatgaa tcggtgttgc gcaaaacgta 5341 tatcatactc gcgcttctct taatgaacgc atatatggtg tttctttctg tgacatctca 5401 atggaaggaa tcttatatga aactgagctg cctgctgccg atttcttcac ggagcttttg 5461 gctcgcccag agtgtcgttt tgtttgtcga tacctgtttg agaagaacgt tattcttttt 5521 tattttaccg ctgttcttat ttggaaacgg aacgctgtca ggggcgcaaa cattgttttg 5581 gctcggcagg ttttcgtttt ttaccgttta ctccattatt ttcggagttg tgctaagcaa 5641 ccacttcgtc aaaaagaaga acttgatgtt tctgctgcat gcggcgatat tcgcctgtgt 5701 atgtctcagc gccgctttga tgccggccgc cacgcttccg ctttgcgcgg tgcatatgct 5761 gtgggcggtg gtcattgact ttcctgtctt tctgcaggcg cctccgcagc agagcaggat 5821 gcattcattt atgcggcgat ctgagttttc gttttacaaa agagaatgga accgatttat 5881 ctcttctaaa gcgatgctgt taaattacgc ggtaatggcg gcgttcagcg gcttcttttc 5941 gttccagatg atgaataccg gcatcttcaa tcagcaagtg atttatatcg tgatttccgc 6001 gcttctgctc atctgctcgc cgatcgccct tttgtactcg attgaaaaaa atgaccgcat 6061 gctgctcatc acgcttccga tcaagcggaa aacgatgttt tgggcgaaat atcgctttta 6121 ttcaggccta ttggcaggcg gatttctcct tgtcgtgatg attgtgggtt tcatcagcgg 6181 ccgtccgatt tcagttctca cttttttgca gtgcattgag ttgctgctgg cgggagcttt 6241 tatcaggtta actgcggata aaaaaaggcc cttcttcagc tggcagacgg agcagcagct 6301 atggagcggt ttctcaaaat atcggtctta tctgttttgt cttcccctat tcttagccac 6361 attagccggc accgctgtat ccttggcggt cattccgata gccggtctgg tcatagtcta 6421 ttacctgcaa aaacaggatg gtggtttctt tgatacaagc aaaagagaac ggcttggaag 6481 ttaacctgct gaagactcat caattttcaa ctatatcaat cgccgcgagc tttctgaaac 6541 cgatcgaaag cgcggcggaa cctgaggaag aaacgattta tttttacggc gccgccgcat 6601 acctgaagga gcaaatcatt gacgatttcg gttgtgctgc cggcagccgt ttcatgtact 6661 cagcgaatct cttttttgat cagcagctga aaacctgcgg gacccgtttg attcacccat 6721 tatataacgg gaatcttcat gttgacgccc tgatgaaaac ttttgcagac ttgtctttcc 6781 cttcttctct ttcattcgaa gcagttgaaa aggccaggaa tgagctgctg ctgaaaatcg 6841 aaaagaaatt cactgatccc ttctcctact cggcggcgcg cttggctgag gaggtcttcg 6901 gaaacccgat gtacggcaca ggcatgtttg gccggaaaga caggataaag gcgattcatc 6961 ctaaacggtt tcacgacgca acggacttta tcgttgattt agcgtcccag caaaaacagc 7021 tgaacatatt gggacaagta caagcatgtg atgttcgggg acatgccccg caaacgtccg 7081 ctgtaacgtc cgaaagaata cccgtaaacc ggcatgtttt tgaaacagag acacgcagcg 7141 ctgcaggtcc ttccgtcttg acactcggat ttgactgcgg agagatgaaa gatgccagtg 7201 attatatcaa aatacagcta atagacggat tactgggaaa gtacggccat tccgctttat 7261 tcaagcactt cagagagaaa gatctggcag tctatcacgt tatcacccgt tacgatgtca 7321 tgaacaattt gctgcttgtt tcaatatgta cagatcagct tcatgagata gacattcccc 7381 cgcgcgtttt ggaagctgtc tcagcttttc atacagatga gcgggaacta gaacaggcaa 7441 agcagttttt acgcaatgaa ttgctgctgc aattcgattc tcccgaaggt ctattggcct 7501 atatggggat cctgcgccgc ttctcctgca cgaaagaagc cttgctggat ggcatctcag 7561 cggttacatg ccgtgatgta ttgcaattca tagcgaccat caattatatc ggagctcatg 7621 tagtaagggg atgaaaagat ggaaaagaaa gccttttttc aacagctgga tgaacgaacc 7681 gatatcagat acacagacag cggactaaaa atattcagat tgaaattccc ccgcgcccac 7741 ctgcggctgt gcaatgtaaa aatcgatttt ggaagccggg atgtatgtat acgggcagaa 7801 tccggtgata cactgcttcc gtatggcaca gcgcattttt tagagcatct tctcttctgg 7861 cataacggcc gcaatctgta cagtgatttt tttgcgcatg gcgcgctttt aaacgcattc 7921 actacctaca ccgataccaa tttcatgttt acctcattac cggatcggct cagacaaacg 7981 atccccatcc tgcttgatgc tttatggaac cactcatttg acaagaagat tgtcgctcag 8041 gaaaaagctg tgattacaag cgaaattcaa acagcacacc ttaaccatca gctctcatat 8101 cattatcagc tgatcagcat gctttctccc tcctcaccag ctgccgtttt ccccgcaggc 8161 agaatcgaag atattgaggc gcttgacatc agtgatttac aaaaagccta taaggctgcc 8221 taccaaaccc atcggatgac gctgtttttg attgggggct cagaaaatac agaaacgctt 8281 ttgcctcctc acctccagct ggaaaagcgt ccagattatc atactgaaag aaaaatcatc 8341 ccggcatgcc ctccagccct ctcacaaaaa atgatgctgg gtgatgagga gcggatggaa 8401 gatacatgga ccggattaca gatcggcgca cttcccgggc aaaacgatct gttgacgatc 8461 aaactttatt gggatatcgc ggctcgtatt ctatttcaat tagattcacc gttttttcaa 8521 gagattcagc aaacgtaccg cctggaaatc gatcgtttgt cagccgagac ttatatctat 8581 gaggatggcg gtttcttgat ccttcacagt caagggaccc attcttcagc ttatatcgat 8641 gtggcttcat actatgtcac acagaagaaa gaacaggctg ccgcctggct tcaatacggc 8701 aaggactctt tgacagacgc gatcatttat gacagcgatt atgtgcgtaa atgcttcgag 8761 tgggcagccg agtgtgacag gtgcgattgc tcgtttcttg acatgtacca catcattcaa 8821 gatatggatg cgcaggtttt cttgtctctc attgatgcaa tggcatcttc taacaaagcg 8881 atcatttaca tttctcagaa agaggcgatc agacaatgaa aaaaaacact gtctttaccc 8941 ttttattgct gcttataggt atggcggctt attccttcgg ctgggttcag gccgttgccg 9001 aagccgccgc ccactatata cagatgatca acaatgatgc tgtacggctc ggactgctgg 9061 cttgtaccgc tgccctcctg atgctgcctg cttttctcta tcttcactat gtcacccaat 9121 ctgtgaaaaa catgacagca gcttttcaga aactcacaca gtctcatcaa agctgctgtg 9181 actttcagca acacaacttg tgcagccgat atgcagaaga cgtcaaatcg ctgcgtgacg 9241 gctataaaaa cgtccgccaa acatacgtga tggctgccgt gttgtgccaa gtcatcatat 9301 tcggctgcat gttcgaaatc gttaaagccg tccctttccg ccttcacacg ccccctgccc 9361 tttcaatggg acttgcgatg ctactgatcc tgtatttatt gttttgcatg cgaacgtatt 9421 tgcggcagct tttccggcac ggcagcctgt tcagaaaagt gtttgcggga gcgctggcag 9481 cagccgggat ttggtggatg ctcagtttca ctataagtga actgctgttt ctgattatcc 9541 tggctgccat ccagcaaatc ggatcattta tttataagcg attctcttat cgcagcaccg 9601 cttctcttga tctataaaat agctacatca caatcgcaga cgggcccgcg ccaaccgaaa 9661 tcaccttcac ggctgtataa tcacttgtgc ggtacacggt cacagcattg tcgttgggct 9721 gtgccgcata aatatactgt ttgtccggcg ttacggcgag gtcaaaagcg ccggggagag 9781 acacgtttgc aatgatcctc tgtctggccg tattaactac atataagtta ttaataagaa 9841 acggttcact atatggctct agaaaataag ctctagtgct atcgagagaa aatacaatcg 9901 tcctcagcgt ttcaaataaa cggctgaagt cccctcccct gcttaatgga gacaaactaa 9961 tctccgttac acctttcggc cacagctcac cattcaacac gtacgcaaaa tttccagcgt 10021 aaggagttgc aattaaactg ggaaattcaa atgtatctat gatactttct accaactgcc 10081 cagtatttac atcatattta tcaaccgacc aactgcttcc attagcggta tagacatact 10141 gtccattcgg atcaagagca aagtctaaag cgccgggtat cgcgattaca tcaattactg 10201 attgattggc cgtgtcaatc atatatatag atccggcggc catcacgtag gcgtactcat 10261 ctgttggatc aaattcgatc tttctcggtc ctcctccaac agtaatggta tttatgatgg 10321 ttaatgtttt attatcaatc actgagacaa ggttggtatt cccatggagg acatacgtga 10381 atgctttatc gaaagtaacg cctagattag ttggcacagc agaaaacagg attgttttta 10441 caacagtgtt taaatctgca tcaatgaccg aaaccgtgct gtctccagta ttggcagtaa 10501 acacatacga ttgagggtct ggcgatggcg gcaaaacaag tgtcccggcc accgtgagcg 10561 gactgccgta gaaaggcggc acgatgagaa gatcttccac tgtcatctga ctaaaatcag 10621 gacagcggtc agactgtgct gcataataaa caacttgatc aatctcttca gtatgaatca 10681 cagaaaccgc cccgtctcct tgtccatatt gatttcgcac aggcgacaca ctgacaagaa 10741 gcataatggt cccttgtact ctcacagcat atcgaacacc aatatcttct gtttccggcc 10801 cagtctggat taagcaaggc tcactgatac aggacacatt gtctgtactc catgtaacag 10861 ccgctgacgc ttcggccgaa tcaaccttga agccctcagg gatatctatc ggcacttgaa 10921 acggcgcttc tcggctaatt tcctcatcac aaaaacagaa acaattttcc tgtaaagcag 10981 aaagatccat tctttttcat gatttcacgt ccttttagaa atgcacaaca tgcagcggtt 11041 aaatcgtaat ggcacccggt gaaaccccaa gagatatcac agtttcctca gaataatcat 11101 ctgtgttgta aacaatgatg ctctgctcta taaaacgagt tgcatacaga aaatcgccat 11161 tcggggtaaa cgcatagtct gttgctgatt gcggaagatt tccaattaca cctgctccat 11221 tcggactgac agaatataaa ttgccagtga agctgtcatg taaatacacc tctgatccac 11281 tcgtaaacat tacatatagc ccgtcaggct ccagctcttg ggtatccctg atcgtaaacg 11341 tatttaaatc aataaccgtc aaataattga ataagaattc ttgttctaat acatacgcat 11401 accgctccgt agaatctgtt tctatggatg atacgataag ctctcgttca atcgttcctg 11461 tgacttcacc ggtagttagg tccacttttt gcactatacc aaaatttaga acataggcga 11521 ataacccatt ggggtcaatt gcaatatcaa aaccttctat atagacctct caacagtcaa 11581 cgtatcaatt ccaatcacat aaatggcagt accggtgaaa acataagcaa actcatgatt 11641 agggataaat ctaatcaatc gaggcggctg atccagcaat atagttgctg tcactgttaa 11701 tgtgtcataa tcgatgacag aaatcacatt attgttggga tgtaagacaa acacagcgct 11761 tttatctggc gtaatttcaa tacctgccgg attgtacgga agagcaatcg ttgtcacaac 11821 agtatgggtg tttgtatcaa tcacagaaac tgattgatca tttggattcg ctgtaaacgc 11881 gtagacgggt tccggctccg gtacaagcac aatcgtcccc gtcaccgata aaggactgcc 11941 gtaaaacggc ggaacaacaa caaggttctc aatcgtgatg ttactgatat ccgggcaatc 12001 atccggttca tcggaataat agacgacttg atcgatttct gcattgtgaa tgacggaaac 12061 agccccatcc ccttgtccat attgatttcg cacaggtgag acactgacga gcagcgtaac 12121 ggttccctgc agacgcactg caaattgaac accgacctca tcaggctctg gccccgtttg 12181 aattaagcaa cggtcactga cacaagaaag gctgtctgca ttccacgtca cgtttgcaac 12241 agcttcagcc ggatcaacga caaatccttc aggtaattca atcggcgctt gaaacatggc 12301 ttctcggctt gcttctccct cacaaaaaca taagcactct tcttgttctg ctcctgcttt 12361 ttctttaaag gaaagcttgt tttttctcat tccttcactt ccttttcaga catacactgc 12421 tcacgcagat ataacagaat atggaagagt aacgtgtttg cttgtacaaa ttactgaggg 12481 taaaagcttc tttttgaaaa tatatctcca aacatgcggt gtaacgaaag aaaaaattgc 12541 ggtttttttg cgcttctcac aagaatgttg atgataaaat atgacatata tagaattatg 12601 aagggaggga tttgcaaacg tgacaggtgt catatcttct tcttccatcg gagaaaagat 12661 taacgaatgg tatatgtaca tacgccgatt cagcataccc gatgcagaat atttgcgacg 12721 agaaatcaag caagagctgg atcaaatgga agaagatcaa gaccttcatt tgtactattc 12781 actgatggag tttcggcaca acctgatgct tgagtacctt gaaccgttag aaaaaatgag 12841 gattgaggaa cagccgagac tgtctgatct gctgcttgag attgataaaa aacaggctcg 12901 tttaacaggt ctgcttgagt actattttaa tttcttcaga ggcatgtatg agctggatca 12961 gcgggaatat ctgtcggcta ttaaattttt caaaaaggcc gaaagcaagc tgatattcgt 13021 taaggatcgg atagagaaag ctgagttttt ctttaagatg tctgaatctt attactatat 13081 gaaacaaacg tatttttcaa tggactatgc acggcaagca tatgaaatat acaaagaaaa 13141 tgaagcttat aatataagat tgctgcagtg tcattcttta tttgccacca attttttaga 13201 tttaaaacag tatgaggatg ccgtctcaca ttttcaaaaa gcttattcta tggcagaagc 13261 tgaaaagcag ccccaattaa tgggaagaac tttgtacaat atcgggcttt gtaaaaacag 13321 ccaaagccaa tatgaggatg ccataacata tttcaaaaga gcaatagctg tttttgaaga 13381 atcaaatatt cttccttcct tacctcaagc gtatttttta attacacaga tccattataa 13441 attaggaaaa atggataaag ctcatgaata tcatagtaag ggaatggctt attcacaaaa 13501 ggccggagat gtaatatatt tatcagagtt tgaatttttg aaatctttat acttatcagg 13561 cccggatgaa gaagcaattc aaggattttt tgattttctc gaaagtaaaa tgttgtatgc 13621 tgatcttgaa gatttcacta ttgatgtggc aaaatattat catgaacgta aaaattttca 13681 aaaagcttct gcttattttt tgaaggtgga acaagtaagg caacttattc aaggaggagt 13741 gagtttgtat gaaattgaag tctaaactat tactctcttg tctggctcta agcactgtgt 13801 tcgtggcaac aactattgca aatgcaccta cacaccaaat tgaagttgca caacgaggaa 13861 tgatttaacc gccgtccatc ggcggttttt ttgtcccctc tttacccagt ctcccaatcc 13921 attctatgat tttttcaata atcttaaaaa tagtggaaat gcagcatgtc tctaaaaagt 13981 gtgagaaccc actttactca atggaatcga gaaaatgatg tgacggagtt cgaaacgtcg 14041 agtgcgacag ttgaacaggc agctgagaca atcggcgtaa gcctgtctag aatcgccaag 14101 tccctgtcct tcagagggga aggagatcag gtgattctga ttgtggcagc cggcgatgcc 14161 aagatcgaca acaaaaagtc caggcaaaca tttggcttta aagcaagaat gctctctcct 14221 aatgaggtac tggagcagac aggccatgaa attggaggag tttgcccaat tggatagact 14281 catgatcctg a //