LOCUS       gnlProkkaFFKAFJCE_14   14270 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FFKAFJCE_14.
ACCESSION   gnlProkkaFFKAFJCE_14
VERSION     gnlProkkaFFKAFJCE_14
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 19:01:23
            Original ID  :: gnl|Prokka|FFKAFJCE_14
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14270
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14270
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2658:4270]"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2659..4270
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
     cand_cluster    1..14270
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /kind="single"
                     /product="sactipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..14270
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="sactipeptide"
                     /region_number="1"
                     /rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /tool="antismash"
     CDS             2..109
                     /locus_tag="ctg14_1"
                     /transl_table=11
                     /translation="MYCRLRGKRNVSEQVLLTAACQNMKKIATYLAKQG"
     CDS             114..287
                     /locus_tag="ctg14_2"
                     /transl_table=11
                     /translation="MGALFLHLKPDEYLKKLYKTKKLVEKTSFSLQAKDKARRLCPFDR
                     DYIFSGADTPIS"
     CDS             complement(251..1921)
                     /locus_tag="ctg14_3"
                     /transl_table=11
                     /translation="MNIAEQMKDVLKEEIKAAVLKAGLAEESQIPNVVLETPKDKTHGD
                     YSTNMAMQLARVAKKAPRQIAEEIVAHFDKGKASIEKLDIAGPGFINFYMNNQYLTKLI
                     PSVLEAGEAYGETNIGNGERVQVEFVSANPTGDLHLGHARGAAVGDSLCNVLSKAGYDV
                     SREYYINDAGNQINNLALSVEVRYFEALGLEKPMPEDGYRGEDIIAIGKRLAEEYGDRF
                     VNEEESERLEFFREYGLKYELEKLRKDLENFRVPFDVWYSETSLYQNGKIDKALEALRE
                     KGHVYEEDGATWFRSTTFGDDKDRVLIKKDGTYTYLLPDIAYHKDKLDRGFDKLINVWG
                     ADHHGYIPRMKAAIEALGYEKGTLEVEIIQLVHLYKNGEKMKMSKRTGKAVTMRDLIEE
                     VGLDAVRYFFAMRSADTHMDFDLDLAVSTSNENPVYYAQYAHARICSMLRQGEEQGLKP
                     AADLDFSHIQSEKEYDLLKTIGGFPEAVAEAAEKRIPHRVTNYIYDLASALHSFYNAEK
                     VIDPENEEKSRARLALMKATQITLNNALQLIGVSAPEKM"
     CDS             complement(1918..2346)
                     /locus_tag="ctg14_4"
                     /transl_table=11
                     /translation="MKQETPITLHVKSVIEDDGNQEVIEFRTTGFYYVKQNKVYLSYYE
                     EHDLGKVKTIVKVSEGEVLVMRSGAVKMNQRFVTGASTIAKYKMSFGELELKTSTKSIQ
                     SDLDEEKGRISIAYDMHVGDEQEHLHNMTITYEGGTHA"
     CDS             2659..2790
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: Subtilosin_A"
                     /gene_functions="biosynthetic-additional (sactipeptides)
                     predicted sactipeptide"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg14_5"
                     /sec_met_domain="Subtilosin_A (E-value: 2.4e-30, bitscore:
                     97.4, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKAVIVENKGCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
     CDS_motif       2659..2688
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKKAVIVENK"
                     /locus_tag="ctg14_5_sactipeptide"
                     /aSTool="sactipeptides"
                     /tool="antismash"
     CDS_motif       2689..2790
                     /prepeptide="core"
                     /locus_tag="ctg14_5_sactipeptide"
                     /peptide="sactipeptide"
                     /predicted_class
                     /score="33.00"
                     /molecular_weight="0.0"
                     /monoisotopic_mass="0.0"
                     /core_sequence="GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
                     /aSTool="sactipeptides"
                     /tool="antismash"
                     /leader_sequence="MKKAVIVENK"
                     /leader_location="[2658:2688](+)"
     CDS             2924..4270
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: subtilosin"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Subtilosin_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SPASM"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg14_6"
                     /sec_met_domain="subtilosin (E-value: 0.0, bitscore:
                     1081.0, seeds: 6, tool: rule-based-clusters)"
                     /sec_met_domain="Subtilosin_rSAM_RRE (E-value: 1.3e-30,
                     bitscore: 98.4, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 8.7e-09, bitscore: 28.4,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 2.8e-23, bitscore: 75.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 1.4e-11, bitscore:
                     37.5, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 4.3e-11, bitscore: 36.0,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFIEQMFPFINESVRVHQLPEGGVLEIDYLRDNVSISDFEYLDLN
                     KTAYELCMRMDGQKTAEQILAEQCAVYDESPEDHKDWYYDMLNMLQNKQVIQLGNRASR
                     HTITTSGSNEFPMPLHATFELTHRCNLKCAHCYLESSPEALGTVSIEQFKKTADMLFDN
                     GVLTCEITGGEIFVHPNANEILDYVCKKFKKVAVLTNGTLMRKESLELLKTYKQKIIVG
                     ISLDSVNSEVHDSFRGRKGSFAQTCKTIKLLSDHGIFVRVAMSVFEKNMWEIHDMAQKV
                     RDLGAKAFSYNWVDDFGRGRDIAHPTKDAEQHRKFMEYEQNVIDEFKDLIPIIPYERKR
                     AANCGAGWKSIVISPFGEVRPCALFPKEFSLGNIFHDSYESIFNSPLVHKLWQAQAPRF
                     SEHCMKDKCPFSGYCGGCYLKGLNSNKYHRKNICSWAKNEQLEDVVQLI"
     CDS             4283..4444
                     /locus_tag="ctg14_7"
                     /transl_table=11
                     /translation="MSPAQRRILLYILSFIFVIGAVVYFVKSDYLFTLIFIAIAILFGI
                     RARKADSR"
     CDS             4441..5160
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 127.7; E-value:
                     9.5e-39)"
                     /gene_kind="transport"
                     /locus_tag="ctg14_8"
                     /transl_table=11
                     /translation="MSILDIHDVSVWYERDNVILEQVDLHLEKGAVYGLLGVNGAGKTT
                     LINTLTGVNRNFSGRFTLCGIEAEAGMPQKTSDQLKTHRYFAADYPLLFTEITAKDYVS
                     FVHSLYQKDFSEQQFASLAEAFHFSKYINRRISELSLGNRQKVVLMTGLLLRAPLFILD
                     EPLVGLDVESIEVFYQKMREYCEAGGTILFSSHLLDVVQRFCDYAAILHNKQIQKVIPI
                     GEETDLRREFFEVIGHE"
     CDS             5153..6463
                     /locus_tag="ctg14_9"
                     /transl_table=11
                     /translation="MNNIIPIMSLLFKQLYSRQGKKDAIRIAAGLVILAVFEIGLIRQA
                     GIDESVLRKTYIILALLLMNAYMVFLSVTSQWKESYMKLSCLLPISSRSFWLAQSVVLF
                     VDTCLRRTLFFFILPLFLFGNGTLSGAQTLFWLGRFSFFTVYSIIFGVVLSNHFVKKKN
                     LMFLLHAAIFACVCISAALMPAATLPLCAVHMLWAVIIDFPVFLQAPPQQSKMHSFMRR
                     SEFSFYKREWNRFISSKAMLLNYAVMAAFSGFFSFQMMNTGIFNQQVVYIVISALLLIC
                     SPIALLYSIEKNDRMLLITLPIKRKTMFWAKYRFYSGLLAGGFLLVVMIVGFISGRPIS
                     VLTFLQCIELLLAGAFIRLTADEKRPSFSWQTEQQLWSGFSKYRSYLFCLPLFLATLAG
                     TAVSLAVIPIAGLVIVYYLQKQDGGFFDTSKRERLGS"
     CDS             6429..7613
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg14_10"
                     /sec_met_domain="Peptidase_M16_C (E-value: 1.5e-06,
                     bitscore: 21.2, seeds: 139, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIQAKENGLEVNLLKTHQFSTISIAASFLKPIESAAEPEEETIYF
                     YGAAAYLKEQIIDAFGCAAGSRFMYSANLFFDQQLKTCGTRLIHPLYNGNLHVDALLKT
                     FADLSFPSSLSFEAVEKARNELLLRIEKKFTDPFSYSAARLAEEVFGNPMYGTGMFGRK
                     DRIKAIHPKRFLDATDFIVDLVSQQKQLNILGQVQACDVRGHAPQTSAVTSERIPVNRH
                     VFETETRSAAGPSVLTLGFDCGEMKDASDYIKIQLIDGLLGKYGHSALFKHFREKDLAV
                     YHVITRYDVMNNLLLVSICTDQLHEIDIPPRVLEAVSAFHTDERELEQAKQFLRNELLL
                     QFDSPEGLLAYMGILRRFSCTKEALLDGISAVTCRDVLQFIATINYIGAHVVRG"
     CDS             7618..8898
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg14_11"
                     /sec_met_domain="Peptidase_M16 (E-value: 2.4e-14, bitscore:
                     46.4, seeds: 24, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENKAFFQQLDERTDIRYTDSGLKIFRLKFPRAHLRLCNVKIDFG
                     SRDVCIRAESGDTLLPYGTAHFLEHLLFWHNGRNLYSDFFAHGALLNAFTTYTDTNFMF
                     TSLPDRLRQTIPILLDALWNHSFDKKIVAQEKAVITSEIQTAHLNDQLSYHYQLISMLS
                     PSSPAAVFPAGRIEDIEALDISDLQKAYKAAYQTHRMTLFLIGGSENTETLLPPHLQLE
                     KRPDYHAERKIIPACPPALSQKMMLGDEERMEDTWTGLQIGALPGQNDLWVIKLYWDIA
                     ARILFQLDSPFFQEIQQTYRLEIDRLSAETYIYEDGGFLILHSQGTHSSAYIDVASYYV
                     TQKKEQVAAWLQYGKDSLTDAIIYDSDYVRKCFEWAAECDRCDCSFLDMYHIIQDMDAQ
                     VFLSLIDAMASSNKAIIHVSQKEAIRQ"
     CDS             8895..9596
                     /locus_tag="ctg14_12"
                     /transl_table=11
                     /translation="MKKSTVFTLLLLLIGMAAYSFGWVQAVAEAAAQYVQMINNDAVRL
                     GLLACTAALLMLPAFLYLHYVTQSVKNMTAAFQKLTQSHQSCCDFQQHNLCSRYAEDVK
                     SLRDSYKNVRQTYVMAAVLCQVIIFGCMFEIVKAVPFRLHTPPALSMGLAMLLILYLLF
                     CMRTYLRQLFRHGSLFRKVFAGALAAAGIWWMLSFSISELLFLIILAAIQQIGSFIYKR
                     FSYRSTASLDL"
     CDS             complement(9602..10978)
                     /locus_tag="ctg14_13"
                     /transl_table=11
                     /translation="MKKNDLSALQENCFCFCDEEISREAPFQVPIDIPKGFKVDSAEAS
                     AAVTWSTDNVSCISEPCLIQTGPEPEDIGVRYAVRVQGTITLLVSVSPVRNQYGQGNGA
                     VSVIHTEEIDQVVYYAAQSGRCPDFSQITVEDLLIVPPFYGSPLTVTGTLVLPPSPDPQ
                     SYVFTANTGDSTVSVIDADLNTVVKTIPFSDVPTNLGVTFDKAFTYVLHGNTNLVSVID
                     NKTLTIINTITVGGGPRKIEFDPKDEFAYVMAAGSIYVINTASQSVIDVIPIPGALDFA
                     LDPNGQYVYTANGSSWSVDKYDINTGQLVESIIDTFEFPSLIATPYAGNFAYVLNGELW
                     PKGVTEISLSPLSRGGDFSRLFETLRTIVFSLDSTRAYFLEPYSEPFLINNLYVVNTAR
                     QRIIANVSLPGAFDLAVTPDKQYIYAAQPNDNAVTVYRTSDYTAVTVIPVGAGPSAIAM
                     "
     CDS             complement(11017..11502)
                     /locus_tag="ctg14_14"
                     /transl_table=11
                     /translation="MFAYVLNFGLVQKVDLSTGEVTGTIERELIVSSIETDSPERYAYV
                     LEQEFLFNYLTVIDLNTFTISNTQELEPDGQYRMFTSGTGVYLYDDFTGNLYSVSPNGA
                     GVIGNLPQSATDYAFTPNGDFLYATRFIEQSIIVYNTDDYSEETVISLGVSPGAITI"
     CDS             complement(11535..12368)
                     /locus_tag="ctg14_15"
                     /transl_table=11
                     /translation="MRKNKLSFKEKAGAEQEECLCFCEGEASREAMFQAPIELPEGFVV
                     DPAEAVANVTWNADSLSCVSDRCLIQTGPEPDEVGVQFAVRLQGTVTLLVSVSPVRNQY
                     GQGDGAVSVIHNAEIDQVVYYSDEPDDCPDISDITIENLVVVPPFYGSPLSVTGTIVLV
                     PEPEPVYAFTANPNDQSVSVIDTNTHTVVTTIALPYNPAGIEITPDKSAVFVLHPNNNV
                     ISVIDYDTLTVTATILLDQPPRLIRFIPNHEFAYVFTGTAIYVIGIDTLTVERSI"
     CDS             12598..13743
                     /locus_tag="ctg14_16"
                     /transl_table=11
                     /translation="MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEE
                     DQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPRLSDLLLEIDKKQARLTGLLEYYFN
                     FFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFSMDY
                     ARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAVSHFQKAYSMAEAEKQPQLMG
                     RTLYNIGLCKNSQSQYEDAITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKA
                     HEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAIQGFFDFLESKMLYADLEDFT
                     IDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV"
     CDS             13727..13846
                     /locus_tag="ctg14_17"
                     /transl_table=11
                     /translation="MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI"
     CDS             13944..14255
                     /locus_tag="ctg14_18"
                     /transl_table=11
                     /translation="MSLKSVRTHFTQWNRENDVTEFETSSATVEQAAETIGVSLSRIAK
                     SLSFRGEGDQVILIVAAGDAKIDNKKSRQTFGFKARMLSPNEVLEQTGHEIGGVCPIG"
ORIGIN
        1 tcgctattgc aggttgaggg gaaaacgaaa tgtgagtgaa caagttctcc tcacagccgc
       61 atgccagaac atgaagaaaa ttgccacata cctagccaag cagggctagg catgtgggag
      121 cgctttttct gcatctgaaa cctgatgaat atctaaagaa actgtataaa acaaaaaagc
      181 ttgtagaaaa aacgtcgttt tctctacaag ctaaggacaa agcccgaaga ctttgtcctt
      241 ttgatcgtga ttacattttt tccggagccg atacgccgat cagctgaagc gcattgttca
      301 gcgtaatttg cgttgccttc attaaagcca ggcgcgcacg gcttttctct tcattttcag
      361 ggtcgatgac tttctctgcg ttgtagaagc tgtgcagagc agaggccaga tcataaatat
      421 agtttgtgac acggtgcgga attcgttttt ccgctgcttc cgccactgcc tctgggaagc
      481 cgccgatcgt tttcagcaga tcgtattctt tttctgattg aatatggctg aaatcaagat
      541 ccgccgccgg tttaagtccc tgctcttccc cttggcgaag catgctgcag attcgggcat
      601 gtgcatattg tgcataatac acagggtttt cgttagatgt tgatacagca agatctaagt
      661 caaaatccat atgggtgtcc gcacttcgca tcgcaaagaa gtaacgaacg gcatccaagc
      721 cgacttcttc aatcaggtca cgcatcgtta cggctttacc ggtccgtttg ctcattttca
      781 ttttctcgcc gtttttgtaa aggtgaacga gctgaatgat ttctacttca agcgttcctt
      841 tttcatagcc cagcgcttcg attgccgctt tcatacgcgg gatgtagccg tggtgatcgg
      901 cgccccaaac gttgatcagc ttatcaaagc cgcggtcaag cttgtcctta tgatacgcga
      961 tatcaggaag aaggtacgtg tacgtgccgt ccttcttgat caatacgcgg tctttatcat
     1021 cgccgaaagt cgtggaacgg aaccaagtcg cgccgtcttc ttcatagaca tggccttttt
     1081 cgcggagcgc ttcaagcgcc ttgtcaattt ttccattttg gtacagtgaa gtttctgaat
     1141 accacacatc aaacggcaca cggaagtttt ccaagtcctt gcgcagcttt tccagctcgt
     1201 atttcaggcc gtattcacgg aaaaactcga ggcgttcact ttcctcttcg ttaacgaaac
     1261 gatcaccgta ttcctcagcg agacgctttc cgatcgcaat gatatcctcg ccgcggtagc
     1321 catcctccgg catcggtttt tccaatccga gtgcttcaaa gtaacggact tcaacagaaa
     1381 gcgccagatt gttgatttgg tttcccgcat cgttaatgta gtattcgcgg cttacatcgt
     1441 aacccgcttt tgaaagcacg ttgcacaagg aatcgccgac agccgccccg cgcgcatgcc
     1501 cgaggtgaag gtctcccgtc ggattcgctg atacgaattc tacctgaact cgttcaccgt
     1561 ttccgatgtt tgtttccccg tatgcttccc ccgcttccag tacagacgga atcagctttg
     1621 ttaaatattg attgttcata tagaaattga taaaacccgg gccggcgata tccagttttt
     1681 caatcgaagc ctttccttta tcaaaatgag cgacaatctc ttcagcgatt tgacgcggcg
     1741 ctttttttgc cactcttgcc agctgcatcg ccatattcgt tgagtagtcg ccgtgtgttt
     1801 tatctttcgg tgtttctaaa acaacattgg gaatctggct ttcttcagcc agtcccgctt
     1861 tcagaacggc cgctttgatt tcttctttca gcacgtcctt catttgttcc gcaatgttca
     1921 tgcgtgagtt cctccctcat acgtgatcgt catattatgt aaatgctcct gctcgtctcc
     1981 gacatgcatg tcatatgcga tgctgatacg gcctttttct tcatcgagat cagattgaat
     2041 cgattttgtg cttgttttca gctcaagctc accaaatgac attttatatt tagcaattgt
     2101 ggaagctccc gttacgaaac gctgattcat tttgacagcg cctgacctca tgaccaggac
     2161 ttcgccttcg cttactttca caatcgtttt gactttgcct aagtcgtgtt cttcataata
     2221 agatagatac actttatttt gttttacata gtaaaatccg gtggtgcgga attcgatgac
     2281 ttcttggttt ccgtcatcct cgatcacaga cttcacatgg agtgttatcg gtgtctcttg
     2341 cttcattctg acacgtcctt ttcatcattc cactttgact tgaatagtat aatcattcgg
     2401 tttgtaaact tcaactgctt ctatcttacc atcattgctc atcagatttg aagataaacc
     2461 tcataaaaag catttcctta tatagaagag aaaatcatat cactaattac ctttaggaaa
     2521 tgttacattt ttccgaaatc tatcatttcc ttttcacatt tttttcaaaa tatatgcatt
     2581 gaattagtaa tttgatagtt ttaagataaa agtacaacat agatctgcta gaaaaacaaa
     2641 aaaagggagg attcaattat gaaaaaagct gtcattgtag aaaacaaagg ttgtgcaaca
     2701 tgctcgatcg gagccgcttg tctagtggac ggtcctatcc ctgattttga aattgccggt
     2761 gcaacaggtc tattcggtct atgggggtaa ggatctccca aaagggcata gtcattctac
     2821 tatgcccttt ttaagtaaat tacaattttt aactaaaatc tacctcttgg atatacatac
     2881 acaaccgttt ttcatttgaa tcatatgtat aggggatggt attttgttta tagagcagat
     2941 gtttccattt attaatgaaa gtgtaagagt tcaccagctt cctgagggcg gcgtgttaga
     3001 aatcgactac ttgcgcgata atgtctccat ttctgacttt gagtatttgg atctcaacaa
     3061 aacggcttac gagctctgca tgcgcatgga tggccaaaaa acagctgagc agattttagc
     3121 tgagcaatgt gcagtgtatg atgaatcacc ggaagaccat aaagattggt attacgacat
     3181 gcttaacatg ctccagaaca agcaggtgat ccagcttgga aaccgggcca gccgccatac
     3241 aatcaccacg agcggcagca atgaatttcc gatgcccctg cacgccacct ttgaactgac
     3301 gcaccgctgt aatttgaaat gcgcccactg ttatttggaa agctcacctg aagcgctcgg
     3361 caccgtgtcg attgagcaat tcaaaaaaac ggctgatatg ctgtttgata acggtgtatt
     3421 gacatgcgaa atcacaggcg gagaaatttt tgtccatcca aacgccaatg agattcttga
     3481 ctatgtgtgt aaaaagttca aaaaagtcgc tgtcttaaca aacggaacac tcatgcgaaa
     3541 agagagcctg gagcttttga agacttacaa gcaaaaaatc atcgtcggca tttctcttga
     3601 tagtgtcaat tccgaggtcc atgactcctt tagagggaga aaaggctctt ttgcccaaac
     3661 ttgtaaaacg ataaaattgt tgagtgacca cggtatattt gtcagagtcg ccatgtctgt
     3721 cttcgaaaaa aacatgtggg aaatccacga tatggcccaa aaggttcggg atctcggggc
     3781 gaaggcattt tcttacaatt gggttgacga tttcggaaga ggcagggata ttgcccatcc
     3841 aacgaaagac gccgagcagc accgcaagtt tatggaatac gagcaaaatg tgattgatga
     3901 gtttaaagat ctgattccga ttattcccta tgagagaaaa cgcgcggcaa attgcggcgc
     3961 tggctggaag tccattgtga tcagcccgtt cggcgaagta cgtccttgcg ccctctttcc
     4021 aaaggaattt tcattgggaa atatttttca tgattcctat gaaagcatct ttaactcccc
     4081 tctcgtccat aaactgtggc aagcgcaggc gccgcggttc agtgaacatt gcatgaaaga
     4141 caaatgcccg ttcagcggct attgcggagg ctgttactta aaagggctga actctaacaa
     4201 atatcaccga aaaaacattt gctcttgggc gaaaaatgaa caattagaag acgtggtcca
     4261 gcttatttag gagggaaacg atttgtcacc agcacaaaga agaattttac tgtatatcct
     4321 ttcatttatc tttgtcatcg gcgcagtcgt ctattttgtc aaaagcgact atctgtttac
     4381 gctgattttc attgccattg ccattctgtt cgggattcgc gcgcggaagg ctgactcgcg
     4441 atgagcattt tggatataca cgatgtatcc gtttggtatg aacgggacaa cgtcatctta
     4501 gaacaagtgg atttacattt agaaaaaggc gccgtttacg ggttgcttgg ggtaaacggc
     4561 gccggaaaaa cgacactgat taatacgctg acaggcgtga accgcaattt cagcgggcgc
     4621 tttacactgt gcggaattga agcggaggcc ggcatgccgc agaaaacatc agatcagctg
     4681 aagactcacc gttacttcgc cgccgattat ccgctgctat ttacagaaat tacggcgaag
     4741 gactatgtgt ctttcgtcca ttcgctttat caaaaggatt tttcagagca gcagtttgcc
     4801 agtttggccg aggcctttca tttttcgaaa tacatcaaca ggagaatttc ggagctttcc
     4861 ttggggaaca ggcaaaaggt tgtattgatg acaggcttat tgctgcgcgc tcccctgttt
     4921 attttggatg agccgctcgt cggtttggat gtggaatcga tagaggtttt ttatcagaaa
     4981 atgcgcgagt actgtgaagc aggcgggacc attttgtttt cttctcatct gctcgatgtc
     5041 gtgcaaagat tttgtgatta tgcggctatt cttcacaaca aacagatcca aaaggtcatt
     5101 ccgattggag aggagaccga tctgcggcgg gaatttttcg aggtgatcgg ccatgaataa
     5161 cataatcccc atcatgtctt tgctgttcaa acagctttac agccggcaag ggaaaaagga
     5221 cgccatccgc attgccgcag gccttgtcat tctggccgtg tttgaaatcg ggctgattcg
     5281 ccaggccggc attgatgaat cggtgttgcg caaaacgtat atcatactcg cgcttctctt
     5341 aatgaacgca tatatggtgt ttctttctgt gacatctcaa tggaaggaat cttatatgaa
     5401 actgagctgc ctgctgccga tttcttcacg gagcttttgg ctcgcccaga gtgtcgtttt
     5461 gtttgtcgat acctgtttga gaagaacttt attctttttt attttaccgc tgttcttatt
     5521 tggaaacgga acgctgtcag gggcgcaaac attgttttgg ctcggcaggt tttcgttttt
     5581 taccgtttac tccattattt tcggagttgt gctaagcaac cacttcgtca aaaagaagaa
     5641 cttgatgttt ctgctgcatg cggcgatatt cgcctgtgta tgtatcagcg ccgctttgat
     5701 gccggccgcc acgcttccgc tttgcgcggt gcatatgctg tgggcggtga tcattgactt
     5761 tcctgtcttt ctgcaggcgc ctccgcagca gagcaagatg cattcattta tgcggcgatc
     5821 tgaattttcg ttttacaaaa gagaatggaa ccgatttatc tcttctaaag cgatgctgtt
     5881 aaattacgcg gtaatggcgg cgttcagcgg cttcttttcg ttccagatga tgaataccgg
     5941 catcttcaat cagcaagtgg tttatatcgt gatttccgcg cttttgctca tctgctcgcc
     6001 gatcgccctt ttgtattcga ttgaaaaaaa tgaccggatg ctgcttatca cgcttccgat
     6061 caagcggaaa acgatgtttt gggcgaaata tcgcttttat tcaggcctat tggcaggcgg
     6121 atttctcctt gtcgtgatga ttgtgggttt catcagcggc cgtccgattt cagttctcac
     6181 ttttttgcag tgcattgagt tgctgctggc gggagctttt atcaggttaa ctgcggatga
     6241 aaaaaggccc tccttcagct ggcagacgga gcagcagcta tggagcggtt tctcaaaata
     6301 tcggtcttat ctgttttgtc ttcccctatt cttagccaca ttagccggca ccgctgtatc
     6361 cttggcggtc attccgatag ccggtctggt catagtctat tacctgcaaa aacaggatgg
     6421 tggtttcttt gatacaagca aaagagaacg gcttggaagt taacctgctg aagactcatc
     6481 aattttcaac tatttcaatc gccgcgagct ttctgaaacc gatcgaaagc gcggcggaac
     6541 ctgaggaaga aacgatttat ttttacggcg ccgccgcata cctgaaggag caaattattg
     6601 acgctttcgg ttgtgctgcc ggcagccgtt tcatgtactc agcgaatctc ttttttgatc
     6661 agcagctgaa aacctgcggg acccgtttga ttcacccatt atataacggg aatcttcatg
     6721 ttgacgccct gctgaaaact tttgcagact tgtctttccc ttcttctctt tcattcgaag
     6781 cagttgaaaa ggccaggaat gagctgctgc tgagaatcga aaagaaattc actgatcctt
     6841 tctcctactc ggcggcgcgc ttggctgagg aggtcttcgg aaacccgatg tacggcacag
     6901 gcatgtttgg ccggaaagac aggataaagg cgattcatcc taaacggttt ctcgacgcaa
     6961 cggactttat cgttgattta gtgtcccagc aaaaacagct gaacatattg ggacaagtac
     7021 aagcatgtga tgttcgggga catgccccgc aaacgtccgc tgtaacgtcc gaaagaatac
     7081 ccgtaaaccg gcatgttttt gaaacagaga cacgcagcgc tgcaggtcct tccgtcttga
     7141 cactcggatt tgactgcgga gagatgaaag atgccagcga ttatatcaaa atacagctaa
     7201 tagacggatt actgggaaag tacggccatt ccgctttatt caagcacttc agagagaagg
     7261 atctggcagt ctatcacgtt atcacccgtt acgatgtcat gaacaatttg ctgcttgttt
     7321 caatatgtac agatcagctt catgagatag acattccccc gcgcgttttg gaagctgtct
     7381 cagcttttca tacagatgag cgggaactag aacaggcaaa gcagttttta cgcaatgaat
     7441 tgctgctgca attcgattct cccgaaggtc tattggccta tatggggatc ctgcgccgct
     7501 tctcctgcac gaaagaagcc ttgctggatg gcatctcagc ggttacatgc cgtgatgtat
     7561 tgcaattcat agcgaccatc aattatatcg gagctcatgt agtaagggga tgaagagatg
     7621 gaaaataaag ccttttttca acagctggat gaacgaaccg atatcagata cacagacagc
     7681 ggactaaaaa tattcagatt gaaattcccc cgcgcccacc tgcggctgtg caatgtaaaa
     7741 atcgattttg gaagccggga tgtatgtata cgggcagaat ccggtgatac actgcttccg
     7801 tatggcacag cgcatttttt agagcatctt ctcttctggc ataacggccg caatctgtac
     7861 agtgattttt ttgcgcatgg cgcgctttta aacgcattca ctacctacac cgataccaat
     7921 ttcatgttta cctcattacc ggatcggctc agacaaacga tccccatcct gcttgatgct
     7981 ttatggaacc actcatttga caagaagatt gtcgctcagg aaaaagctgt gattacaagc
     8041 gaaattcaaa cagcacacct taacgatcag ctctcatatc attatcagct gatcagcatg
     8101 ctttctccct cctcaccagc tgccgttttc cccgcaggca gaatcgaaga tattgaggcg
     8161 ctggacatca gtgatttaca aaaagcctat aaggctgcct accaaaccca tcggatgacg
     8221 ctgtttttga ttgggggctc agaaaataca gaaacgcttt tgcctcctca cctccagctg
     8281 gaaaagcgtc cggattatca tgctgaaaga aaaatcatcc cggcatgccc tccagccctc
     8341 tcacaaaaaa tgatgctggg tgatgaggag cggatggaag atacatggac cggattacag
     8401 atcggcgcac ttcccgggca aaacgatctg tgggtgataa agctttattg ggatatcgcg
     8461 gctcgtattc tatttcaatt agattcaccg ttttttcaag agattcagca aacgtaccgt
     8521 ctggaaatcg atcgcttgtc agccgagact tatatatatg aggatggcgg tttcttgatc
     8581 cttcacagtc aagggaccca ttcttcagct tatatcgatg tggcttcata ctatgtcaca
     8641 cagaagaaag aacaggttgc cgcctggctt caatacggca aggactcttt gacagacgcg
     8701 atcatttatg acagcgatta tgtgcgtaaa tgcttcgagt gggcagccga gtgtgacagg
     8761 tgcgattgct cgtttcttga catgtaccac atcattcaag atatggatgc gcaggttttc
     8821 ttgtctctca ttgatgcaat ggcatcttct aacaaagcga tcattcacgt ttctcagaaa
     8881 gaggcgatca gacaatgaaa aaaagcactg tctttaccct tttattgctg cttataggta
     8941 tggcggctta ttccttcgga tgggttcagg ccgttgccga agccgccgcc caatatgtac
     9001 agatgatcaa caatgatgct gttcggctcg gactgctggc ttgtaccgct gccctcctga
     9061 tgctgcctgc ttttctatat cttcattatg tcacccaatc cgtgaaaaac atgacagcag
     9121 cttttcagaa actcacacag tctcatcaaa gctgctgtga ctttcagcaa cacaacttgt
     9181 gcagccgata tgcagaagac gtcaaatcgc tgcgtgacag ctataaaaac gtccgccaaa
     9241 catacgtgat ggctgccgtg ttgtgccaag tcatcatatt cggctgcatg ttcgaaatcg
     9301 ttaaagccgt ccctttccgc cttcacacgc cccctgccct ttcaatggga cttgcgatgc
     9361 tactgattct gtatttattg ttttgcatgc gaacttattt gcggcagctt ttccggcacg
     9421 gcagcctgtt cagaaaagtg tttgcgggag cgctggcagc agccgggatt tggtggatgc
     9481 tcagtttctc tataagtgaa ctgctgtttc tgattatcct ggctgccatt cagcaaatcg
     9541 gatcatttat ttataaacga ttctcttatc gcagcaccgc ttctcttgat ctataaaata
     9601 gctacatcgc aatcgcagac ggacccgcgc caaccggaat caccgtcacg gctgtataat
     9661 cacttgtgcg gtacacggtc acagcattgt cgttgggctg tgccgcataa atatactgtt
     9721 tgtccggcgt tacggcgagg tcaaaagcgc cggggagaga cacgtttgca atgatcctct
     9781 gtctggccgt attaactaca tataagttat taataagaaa cggttcacta tatggctcta
     9841 gaaaataagc tctagtgcta tcgagagaaa atacaatcgt cctcagcgtt tcaaataaac
     9901 ggctgaagtc ccctcccctg cttaatggag acaaactaat ctccgttaca cctttcggcc
     9961 acagctcacc attcaacacg tacgcaaaat ttccagcgta aggagttgca attaaactgg
    10021 gaaattcaaa tgtatctatg atactttcta ccaactgccc agtatttata tcatatttat
    10081 caaccgacca actgcttcca ttagcggtat agacatactg tccattcgga tcaagagcaa
    10141 agtctaaagc gccgggtatc ggaatgacat caatgaccga ttgagaggcc gtgttaatca
    10201 catatataga tccggcggcc atcacgtatg cgaactcatc ttttggatca aattcgatct
    10261 ttctcggccc tcctccaaca gtaatggtat ttatgatggt taatgtttta ttatcaatca
    10321 ctgagacaag gttggtattt ccatggagga catacgtgaa tgctttatcg aaagtaacgc
    10381 ctagattagt tggcacgtca gaaaaaggga ttgtttttac aacagtgttt aaatctgcat
    10441 caataaccga aaccgtgctg tctccagtat tggcagtaaa cacatacgat tgagggtctg
    10501 gtgatggcgg taaaacaagt gtcccggtca ccgtaagcgg actgccgtag aaaggcggta
    10561 caatgagcag atcttccact gttatttgac tgaaatcagg acagcggcca gactgtgctg
    10621 cataataaac aacttgatca atctcttcgg tatgaatcac agaaaccgcc ccattgcctt
    10681 gtccatattg atttcgcaca ggcgacacac tgacaagaag cgtaatggtc ccttgtactc
    10741 tcacagcata tcgaacaccg atatcttctg gttccggccc agtctggatt aagcaaggct
    10801 cactgataca ggacacattg tctgtactcc atgtaacagc cgctgacgct tcggctgaat
    10861 caaccttgaa gcccttaggg atatctattg gcacttgaaa cggcgcttcc cggctaattt
    10921 cctcatcaca aaaacagaaa caattttcct gtaaagcaga aagatcattc tttttcatga
    10981 attcacgtcc ttttagaaat gcacaacatg cagcggttaa atcgtaatgg cacccggtga
    11041 aaccccaaga gatatcacag tttcctcaga ataatcatct gtgttgtaaa caatgatgct
    11101 ctgctctata aaacgagttg catacagaaa atcgccattc ggggtaaacg catagtctgt
    11161 tgctgattgc ggaagatttc caattacacc tgctccattc ggactgacag aatataaatt
    11221 gccggtaaag tcgtcatata aatacacacc tgttccgctc gtaaacattc tatattggcc
    11281 gtcaggctcc agttcttggg tattgctgat cgtaaacgta tttaagtcaa taaccgtcaa
    11341 ataattgaat aagaattctt gttctaagac atacgcatac cgctccggag aatctgtttc
    11401 tatggatgat acgataagct ctcgttcaat cgttcctgtg acttcaccgg tggataagtc
    11461 cactttttgc actaacccaa aattaagaac ataggcgaac aacccattgg ggtcaattgc
    11521 aatatcaaaa ccttctatat agacctctca acagtcaacg tatcaattcc aatcacataa
    11581 atggcagtac cggtgaaaac ataagcaaac tcatgattag ggataaatct aatcaatcga
    11641 ggcggctgat ccagcaatat agttgctgtc actgttaatg tgtcataatc gatgacagaa
    11701 atcacattat tgttgggatg taagacaaac acagcgcttt tatctggcgt aatttcaata
    11761 cctgccggat tgtacggaag agcaatcgtt gtcacaacag tatgggtgtt tgtatcaatc
    11821 acagaaactg attgatcatt tggattcgct gtaaacgcgt agacgggttc cggctccggt
    11881 acaagcacaa tcgtccccgt caccgataaa ggactgccgt aaaacggcgg aacaacaaca
    11941 aggttctcaa tcgtgatgtc actgatatcc gggcaatcat ccggttcatc ggaataatag
    12001 acgacttgat cgatttctgc attgtgaatg acggaaacag ccccgtcccc ttgtccatat
    12061 tgatttcgca caggtgagac actgacgagt agcgtaacgg ttccctgcag gcgcactgca
    12121 aattgaacac cgacctcatc aggctctggc cccgtttgaa ttaagcaacg gtcactgaca
    12181 caagaaaggc tgtctgcatt ccacgtcacg tttgcaacag cttcagccgg atcaacgaca
    12241 aatccttcag gtaactcaat cggcgcttga aacatggctt ctcggcttgc ttctccctca
    12301 caaaaacata agcactcttc ttgttctgct cctgcttttt ctttaaagga aagcttgttt
    12361 tttctcattc cttcacttcc ttttcagaca tacactgctc acgcagatat aacagaatat
    12421 ggaagagtaa cgtgtttgct tgtacaaatt actgagggta aaagcttctt tttgaaaata
    12481 tatctccaaa catgcggtgt aacgaaagaa aaaattgcgg tttttttgcg cttctcacaa
    12541 gaatgttgat gataaaatat gacatatata gaattatgaa gggagggatt tgcaaacgtg
    12601 acaggtgtca tatcttcttc ttccatcgga gaaaagatta acgaatggta tatgtacata
    12661 cgccgattca gcatacccga tgcagaatat ttgcgacgag aaatcaagca agagctggat
    12721 caaatggaag aagatcaaga ccttcatttg tactattcac tgatggagtt tcggcacaac
    12781 ctgatgcttg agtaccttga accgttagaa aaaatgagga ttgaggaaca gccgagactg
    12841 tctgatctgc tgcttgagat tgataaaaaa caggctcgtt taacaggtct gcttgagtac
    12901 tattttaatt tcttcagagg catgtatgag ctggatcagc gggaatatct gtcggctatt
    12961 aaatttttca aaaaggccga aagcaagctg atattcgtta aggatcggat agagaaagct
    13021 gagtttttct ttaagatgtc tgaatcttat tactatatga aacaaacgta tttttcaatg
    13081 gactatgcac ggcaagcata tgaaatatac aaagaaaatg aagcttataa tataagattg
    13141 ctgcagtgtc attctttatt tgccaccaat tttttagatt taaaacagta tgaggatgcc
    13201 gtctcacatt ttcaaaaagc ttattctatg gcagaagctg aaaagcagcc ccaattaatg
    13261 ggaagaactt tgtacaatat cgggctttgt aaaaacagcc aaagccaata tgaggatgcc
    13321 ataacatatt tcaaaagagc aatagctgtt tttgaagaat caaatattct tccttcctta
    13381 cctcaagcgt attttttaat tacacagatc cattataaat taggaaaaat ggataaagct
    13441 catgaatatc atagtaaggg aatggcttat tcacaaaagg ccggagatgt aatatattta
    13501 tcagagtttg aatttttgaa atctttatac ttatcaggcc cggatgaaga agcaattcaa
    13561 ggattttttg attttctcga aagtaaaatg ttgtatgctg atcttgaaga tttcactatt
    13621 gatgtggcaa aatattatca tgaacgtaaa aattttcaaa aagcttctgc ttattttttg
    13681 aaggtggaac aagtaaggca acttattcaa ggaggagtga gtttgtatga aattgaagtc
    13741 taaactatta ctctcttgtc tggctctaag cactgtgttc gtggcaacaa ctattgcaaa
    13801 tgcacctaca caccaaattg aagttgcaca acgaggaatg atttaaccgc cgtccatcgg
    13861 cggttttttt gtcccctctt tacccagtct cccaatccat tctatgattt tttcaataat
    13921 cttaaaaata gtggaaatgc agcatgtctc taaaaagtgt gagaacccac tttactcaat
    13981 ggaatcgaga aaatgatgtg acggagttcg aaacgtcgag tgcgacagtt gaacaggcag
    14041 ctgagacaat cggcgtaagc ctgtctagaa tcgccaagtc cctgtccttc agaggggaag
    14101 gagatcaggt gattctgatt gtggcagccg gcgatgccaa gatcgacaac aaaaagtcca
    14161 ggcaaacatt tggctttaaa gcaagaatgc tctctcctaa tgaggtactg gagcagacag
    14221 gccatgaaat tggaggagtt tgcccaattg gatagactca tgatcctgag
//
