LOCUS       gnlProkkaHGDKEKEP_127   1704 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HGDKEKEP_127.
ACCESSION   gnlProkkaHGDKEKEP_127
VERSION     gnlProkkaHGDKEKEP_127
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 19:13:43
            Original ID  :: gnl|Prokka|HGDKEKEP_127
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 1704
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..1704
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:1704]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..1704
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..1704
                     /SMILES="NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..1704
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /tool="antismash"
     CDS             complement(1..1704)
                     /NRPS_PKS="Domain: AMP-binding (1-369). E-value: 4.3e-101.
                     Score: 330.3. Matches aSDomain:
                     nrpspksdomains_ctg123_1_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (474-542). E-value: 2.8e-28. Score:
                     89.8. Matches aSDomain: nrpspksdomains_ctg123_1_PCP.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     324.9; E-value: 1.2e-98)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg123_1"
                     /sec_met_domain="AMP-binding (E-value: 2.2e-116, bitscore:
                     374.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.9e-20, bitscore:
                     58.1, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWTYGELNAKANRLARILMDCGISPDDRVGILTKPSLEMSAAVLG
                     VLKAGAAFVPIDPDYPDQRIEYILQDSGAKLLLKQEGISVPDSFSGDVILLDGSRTILS
                     LPLDENDEENPETAVTAENLAYMIYTSGTTGQPKGVMVEHHALVNLCFWHHDAFSMTAE
                     DRSAKYAGFGFDASIWEMFPTWTIGAELHVIDEAIRLDIVRLNDYFETNGVTITFLPTQ
                     LAEQFMELENTSLRVLLTGGDKLKRAVKKPYTLVNNYGPTENTVVATSAEIHPEEGSLS
                     IGRAIANTRVYILGEGNQVQPEGVAGELCVAGRGLARGYLNREDETAKRFVADPFVPGE
                     RMYRTGDLVKWTGGGIEYIGRIDQQVKVRGYRIELSEIEVQLAQLSEVQDAAVTAVKDK
                     GGNTAIAAYVTPETADIEALKSALKETLPDYMIPAFWVTLNELPVTANGKVDRKALPEP
                     DIEAGSGEYKAPTTDMEELLAGIWQDVLGMSEVGVTDNFFSLGGDSIKGIQMASRLNQH
                     GWKLEMKDLFQHPTIEELTQYVERAEGKQADQGPVEGEVILTPIQRWFFEK"
     CDS_motif       complement(4..33)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg123_1_0006"
                     /evalue="3.50E-05"
                     /label="NRPS-E1"
                     /locus_tag="ctg123_1"
                     /protein_end="567"
                     /protein_start="557"
                     /score="16.4"
                     /tool="antismash"
                     /translation="LTPIQRWFFE"
     aSDomain        complement(79..282)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg123_1_PCP.1"
                     /evalue="2.80E-28"
                     /label="ctg123_1_PCP.1"
                     /locus_tag="ctg123_1"
                     /protein_end="542"
                     /protein_start="474"
                     /score="89.8"
                     /tool="antismash"
                     /translation="EELLAGIWQDVLGMSEVGVTDNFFSLGGDSIKGIQMASRLNQHGW
                     KLEMKDLFQHPTIEELTQYVERA"
     aSModule        complement(79..1701)
                     /complete
                     /domains="nrpspksdomains_ctg123_1_AMP-binding.1"
                     /domains="nrpspksdomains_ctg123_1_PCP.1"
                     /locus_tags="ctg123_1"
                     /monomer_pairings="Leu -> Leu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(568..633)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg123_1_0005"
                     /evalue="1.10E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg123_1"
                     /protein_end="379"
                     /protein_start="357"
                     /score="36.7"
                     /tool="antismash"
                     /translation="YIGRIDQQVKVRGYRIELSEIE"
     aSDomain        complement(598..1701)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg123_1_AMP-binding.1"
                     /evalue="4.30E-101"
                     /label="ctg123_1_AMP-binding.1"
                     /locus_tag="ctg123_1"
                     /protein_end="369"
                     /protein_start="1"
                     /score="330.3"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="WTYGELNAKANRLARILMDCGISPDDRVGILTKPSLEMSAAVLGV
                     LKAGAAFVPIDPDYPDQRIEYILQDSGAKLLLKQEGISVPDSFSGDVILLDGSRTILSL
                     PLDENDEENPETAVTAENLAYMIYTSGTTGQPKGVMVEHHALVNLCFWHHDAFSMTAED
                     RSAKYAGFGFDASIWEMFPTWTIGAELHVIDEAIRLDIVRLNDYFETNGVTITFLPTQL
                     AEQFMELENTSLRVLLTGGDKLKRAVKKPYTLVNNYGPTENTVVATSAEIHPEEGSLSI
                     GRAIANTRVYILGEGNQVQPEGVAGELCVAGRGLARGYLNREDETAKRFVADPFVPGER
                     MYRTGDLVKWTGGGIEYIGRIDQQVKVR"
     CDS_motif       complement(712..801)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg123_1_0004"
                     /evalue="2.10E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg123_1"
                     /protein_end="331"
                     /protein_start="301"
                     /score="52.0"
                     /tool="antismash"
                     /translation="PEGVAGELCVAGRGLARGYLNREDETAKRF"
     CDS_motif       complement(904..933)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg123_1_0003"
                     /evalue="2.90E-03"
                     /label="NRPS-A_a5"
                     /locus_tag="ctg123_1"
                     /protein_end="267"
                     /protein_start="257"
                     /score="10.2"
                     /tool="antismash"
                     /translation="NYGPTENTVV"
     CDS_motif       complement(1273..1332)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg123_1_0002"
                     /evalue="3.70E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg123_1"
                     /protein_end="144"
                     /protein_start="124"
                     /score="34.7"
                     /tool="antismash"
                     /translation="AYMIYTSGTTGQPKGVMVEH"
     CDS_motif       complement(1534..1575)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg123_1_0001"
                     /evalue="4.90E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="ctg123_1"
                     /protein_end="57"
                     /protein_start="43"
                     /score="19.6"
                     /tool="antismash"
                     /translation="LGVLKAGAAFVPID"
ORIGIN
        1 cttttcaaag aaccagcgct ggatcggcgt caggatgact tcgccctcca ccgggccttg
       61 gtctgcctgt ttgccttcgg cacgctctac gtactgggta agctcttcga tcgtcgggtg
      121 ctggaagaga tctttcattt ccagcttcca gccgtgctgg ttcaagcggc tcgccatttg
      181 aattcctttg atggaatctc cgccaagcga gaagaaattg tcggtgacac cgacttcaga
      241 cattccaagc acgtcctgcc agatgccggc aagcagctct tccatgtcgg tcgtcggcgc
      301 tttgtattct ccgcttcccg cttcgatgtc cggctcaggc aaggctttgc ggtccacttt
      361 gccgtttgcc gtaaccggaa gctcgttcag cgtcacccag aacgccggga tcatgtaatc
      421 cggcagggtt tccttcagtg ctgatttcag tgcttctatg tcagctgttt ccggtgtgac
      481 atacgccgcg atcgctgtgt tgcctccttt atctttgaca gctgtcaccg ccgcatcctg
      541 cacctcagaa agctgggcga gctggacttc aatttctgaa agctcgatcc ggtagccgcg
      601 gaccttgacc tgctggtcga tccggccgat gtattcgatg ccgccgcccg tccatttcac
      661 caaatcgccg gtgcggtaca tgcgctcacc cggcacaaac ggatcagcga caaaccgctt
      721 cgcggtttcg tcttctcgat tcagatagcc tcgcgctaat ccgcgtcccg ccacgcaaag
      781 ctctccggct acgccttccg gctgcacctg attgccctcg ccgagaatgt acactctcgt
      841 attggcaatg gcccgtccga tggaaagcga accttcctcc ggatggattt ctgcgcttgt
      901 ggcaacgacc gtgttttctg tcggcccgta gttgttgacg agtgtgtacg gctttttcac
      961 ggcgcgcttc agcttgtctc cgcctgtcaa gagaacacgg agtgatgtgt tctcaagctc
     1021 catgaactgt tccgcaagct gtgtcggcag gaacgtgatc gttacgccgt tcgtttcaaa
     1081 ataatcgttc aggcggacga tatcgaggcg gatcgcttca tcaatgacgt gaagttcggc
     1141 gccgatcgtc caggtcggga acatctccca aatggaagcg tcgaacccga agcccgcgta
     1201 cttcgcactg cggtcctccg ctgtcatgct gaacgcgtcg tggtgccaga agcacaggtt
     1261 cacaagcgca tggtgctcga ccatgacacc cttcggctgt ccggtcgttc ctgacgtgta
     1321 aatcatgtac gccaagttct ccgcggttac agccgtctct ggattttcct catcgttttc
     1381 atcaagcggc aagcttagaa tcgtgcggct tccgtcgaga agaataacgt ctcctgagaa
     1441 gctatccggc actgaaatgc cttcttgctt caagagaagc ttcgcgccgc tgtcctgtaa
     1501 aatatactca atccgctgat ccgggtagtc aggatcaatc ggcacaaacg ccgctcccgc
     1561 ttttaagacg ccaagcaccg cggcggacat ttcaagcgac ggcttcgtga gaatgccgac
     1621 gcggtcatcc gggctgatgc cgcagtccat cagaatccgc gcgagacggt ttgccttcgc
     1681 attcagctcg ccgtacgtcc aaga
//
