LOCUS       gnlProkkaDFNNBLGB_36   14224 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DFNNBLGB_36.
ACCESSION   gnlProkkaDFNNBLGB_36
VERSION     gnlProkkaDFNNBLGB_36
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 19:13:57
            Original ID  :: gnl|Prokka|DFNNBLGB_36
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14224
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14224
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2612:4224]"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2613..4224
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
     cand_cluster    1..14224
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /kind="single"
                     /product="sactipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..14224
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="sactipeptide"
                     /region_number="1"
                     /rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /tool="antismash"
     CDS             66..239
                     /locus_tag="ctg35_1"
                     /transl_table=11
                     /translation="MGALFLHLKPDEYLKKLYKTKKLVEKTSFSLQAKDKARRLCPFDR
                     DYIFSGADTPIS"
     CDS             complement(203..1873)
                     /locus_tag="ctg35_2"
                     /transl_table=11
                     /translation="MNIAEQMKDVLKEEIKAAVLKAGLAEESQIPNVVLETPKDKTHGD
                     YSTNMAMQLARVAKKAPRQIAEEIVAHFDKGKASIEKLDIAGPGFINFYMNNQYLTKLI
                     PSVLEAGEAYGETNIGNGERVQVEFVSANPTGDLHLGHARGAAVGDSLCNVLSKAGYDV
                     SREYYINDAGNQINNLALSVEVRYFEALGLEKPMPEDGYRGEDIIAIGKRLAEEYGDRF
                     VNEEESERLAFFREYGLKYELEKLRKDLENFRVPFDVWYSETSLYQNGKIDKALEALRE
                     KGHVYEEDGATWFRSTTFGDDKDRVLIKKDGTYTYLLPDIAYHKDKLDRGFDKLINVWG
                     ADHHGYIPRMKAAIEALGYEKGTLEVEIIQLVHLYKNGEKMKMSKRTGKAVTMRDLIEE
                     VGLDAVRYFFAMRSADTHMDFDLDLAVSTSNENPVYYAQYAHARICSMLRQGEEQGLKP
                     AADLDFSHIQSEKEYDLLKTIGGFPEAVAEAAEKRIPHRVTNYIYDLASALHSFYNAEK
                     VIDPENEEKSRARLALMKATQITLNNALQLIGVSAPEKM"
     CDS             complement(1870..2298)
                     /locus_tag="ctg35_3"
                     /transl_table=11
                     /translation="MKQETPITLHVKSVIEDDGNQEVIEFRTTGFYYVKQNKVYLSYYE
                     EHDLGKVKTIVKVSEGEVLVMRSGAVKMNQRFVKGASTIAKYKMSFGELELKTSTKSIQ
                     SDLDEEKGRISIAYDMHVGDEQEHLHNMTITYEGGTHA"
     CDS             2613..2744
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: Subtilosin_A"
                     /gene_functions="biosynthetic-additional (sactipeptides)
                     predicted sactipeptide"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg35_4"
                     /sec_met_domain="Subtilosin_A (E-value: 2e-30, bitscore:
                     97.4, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKAVIVENKGCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
     CDS_motif       2613..2642
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKKAVIVENK"
                     /locus_tag="ctg35_4_sactipeptide"
                     /aSTool="sactipeptides"
                     /tool="antismash"
     CDS_motif       2643..2744
                     /prepeptide="core"
                     /locus_tag="ctg35_4_sactipeptide"
                     /peptide="sactipeptide"
                     /predicted_class
                     /score="32.00"
                     /molecular_weight="0.0"
                     /monoisotopic_mass="0.0"
                     /core_sequence="GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
                     /aSTool="sactipeptides"
                     /tool="antismash"
                     /leader_sequence="MKKAVIVENK"
                     /leader_location="[2612:2642](+)"
     CDS             2947..4224
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: subtilosin"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Subtilosin_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SPASM"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg35_5"
                     /sec_met_domain="subtilosin (E-value: 0.0, bitscore:
                     1022.7, seeds: 6, tool: rule-based-clusters)"
                     /sec_met_domain="Subtilosin_rSAM_RRE (E-value: 2.6e-28,
                     bitscore: 90.8, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 7e-09, bitscore: 28.5,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 2.1e-23, bitscore: 76.0,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 1e-11, bitscore: 37.6,
                     seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 3.3e-11, bitscore: 36.1,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLEIDYLRDNVSISDFEYLDLNKTAYELCMRMDGQKTAEQILAEQ
                     CAVYDESPEDHKDWYYDMLNMLQNKQVIQLGNRAIRHTITTSGSNEFPMPLHATFELTH
                     RCNLKCAHCYLESSPEALGTVSIEQFKKTADMLFDNGVLTCEITGGEIFVHPNANEILD
                     YVCKKFKKVAVLTNGTLMRKESLELLKTYKQKIIVGISLDSVNSEVHDSFRGRKGSFAQ
                     TCKTIKLLSDHGIFVRVAMSVFEKNMWEIHDMAQKVRDLGAKAFSYNWVDDFGRGRDII
                     HPTKDAEQHRKFMEYEQNVIDEFKDLIPIIPYERKRAANCGAGWKSIVISPFGEVRPCA
                     LFPKEFSLGNIFHDSYESIFNSPLVHKLWQAQAPRFSEHCMKDKCPFSGYCGGCYLKGL
                     NSNKYHRKNICSWAKNEQLEDVVQLI"
     CDS             4237..4398
                     /locus_tag="ctg35_6"
                     /transl_table=11
                     /translation="MSPAQRRILLYILSFIFVIGAVVYFVKSDYLFTLIFIAIAILFGM
                     RARKADSR"
     CDS             4395..5114
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 127.7; E-value:
                     9.5e-39)"
                     /gene_kind="transport"
                     /locus_tag="ctg35_7"
                     /transl_table=11
                     /translation="MSILDIHDVSVWYERDNVILEQVDLHLEKGAVYGLLGVNGAGKTT
                     LINTLTGVNRNFSGRFTLCGIEAEAGMPQKTSDQLKTHRYFAADYPLLFTEITAKDYVS
                     FVHSLYQKDFSEQQFASLAEAFHFSKYINRRISELSLGNRQKVVLMTGLLLRAPLFILD
                     EPLVGLDVESIEVFYQKMREYCEAGGTILFSSHLLDVVQRFCDYAAILHNKQIQKVIPI
                     GEETDLRREFFEVIGHE"
     CDS             5107..6417
                     /locus_tag="ctg35_8"
                     /transl_table=11
                     /translation="MNNIIPIMSLLFKQLYSREGKKDAIRIAAGLVILAVFEIGLIRQA
                     GIDESVLRKTYIILALLLMNAYMVFLSVTSQWKESYMKLSCLLPISSRSFWLAQSVVLF
                     VDTCLRRTLFFFILPLFLFGNGTLSGAQTLFWLGRFSFFTVYSIIFGVVLSNHFVKKKN
                     LMFLLHAAIFACVCLSAALMPAATLPLCAVHMLWAVIIDFPVFLQAPPQQGKIHSFMRG
                     SEFSFYKREWNRFISSKAMLLNYAVMAAFSGFFSFQMMNTGIFNQQVIYIVISALLLIC
                     SPIALLYSIEKNDRMLLITLPIKRKTMFWAKYRFYSGLLAGGFLLVVMIVGFISGRPIS
                     VLTFLQCIELLLAGAFIRLTADEKRPSFSWQTEQQLWSGFSKYRSYLFCLPLFLATLAG
                     TAVSLAVIPIAGLVIVYYLQKQDGGFFDTSKRERLGS"
     CDS             6383..7567
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg35_9"
                     /sec_met_domain="Peptidase_M16_C (E-value: 1.2e-06,
                     bitscore: 21.2, seeds: 139, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIQAKENGLEVNLLKTHQFSTISIAASFLKPIESAAEPEEETIYF
                     YGAAAYLKEQIIDAFGCAAGSRFMYSANLFFDQQLKTCGTRLIHPLYNGNLHVDALMKT
                     FADLSFPSSLSFEAVEKARNELLLKIEKKFTDPFSYSAARLAEEVFGNPMYGTGMFGRK
                     DRIKAIHPKRFHDATDFIVDLVSQQKQLNILGQVQACDVRGHAPQTSAVTSERIPVNRH
                     VFETETRSAAGPSVLTLGFDCGEMKDASDYIKIQLIDGLLGKYGHSALFKHFREKDLAV
                     YHVITRYDVMNNLLLVSICTDQLHEIDIPPRVLEAVSAFHTDERELEQAKQFLRNELLL
                     QFDSPEGLLAYMGILRRFSCTKEALLDGISAVTCRDVLQFIATINYIGAHVVRG"
     CDS             7572..8852
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg35_10"
                     /sec_met_domain="Peptidase_M16 (E-value: 5.4e-15, bitscore:
                     48.2, seeds: 24, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKKAFFQQLDERTDIRYTDSGLKIFRLKFPRAHLRLCNVKIDFG
                     SRDVCIRAESGDTLLPYGTAHFLEHLLFWHNGRNLYSDFFAHGALLNAFTTYTDTNFMF
                     TSLPDRLRQTIPILLDALWNHSFDKKIVAQEKAVITSEIQTAHLNHQLSYHYQLISMLS
                     PSSPAAVFPAGRIEDIEALDISDLQKAYKAAYQTHRMTLFLIGGSENTETLLPPHLQLE
                     KRPDYHAERKIIPACPPALSQKMMLGDEERMEDTWTGLQIGALPGQNDLLTIKLYWDIA
                     ARILFQLDSPFFQEIQQTYRLEIDRLSAETYIYEDGGFLILHSQGTHSSAYIDVASYYV
                     TQKKEQAAAWLQYGKDSLTDAIIYDSDYVRKCFEWAAECDRCDCSFLDMYHIIQDMDAQ
                     VFLSLIDAMASSNKAIIHVSQKEAIRQ"
     CDS             8849..9550
                     /locus_tag="ctg35_11"
                     /transl_table=11
                     /translation="MKKNTVFTLLLLLIGMAAYSFGWVQAVAEAAAHYIQMINNDAVRL
                     GLLACTAALLMLPAFLYLHYVTQSVKNMTAAFQKLTQSHQSCCDFQQHNLCSRYAEDVK
                     SLRDGYKNVRQTYVMAAVLCQVIIFGCMFEIVKAVPFRLHTPPALSMGLAMLLILYLLF
                     CMRTYLRQLFRHGSLFRKVFAGVLAAAGIWWMLSFTISELLFLIILAAIQQIRSFIYKR
                     FSYRSTASLDL"
     CDS             complement(9556..10932)
                     /locus_tag="ctg35_12"
                     /transl_table=11
                     /translation="MKKNDLSALQENCFCFCDEEISREAPFQVPIDIPKGFKVDSAEAS
                     AAVTWSTDNLSCVSEPCLIQTGPETEDIGVRYAVRVQGTIMLLVSVSPVRNQYGQGDGA
                     VSVIHTEEIDQVVYYAAQSDRCPDFSQMTVEDLLIVPPFYGSPLTVAGTLVLPPSPDPQ
                     SYVFTANTGDSTVSVIDADLNTVVKTILFSAVPTNLGVTFDKAFTYVLHGNTNLVSVID
                     NKTLTIINTITVGGGPRKIEFDPTDEYAYVMAAGSIYMIDTANQSVIDVIAIPGALDFA
                     LDPNGQYVYTANGSSWSVDKYDVNTGQLVESIIDSFEFPSLIATPYAGNFAYVLNGELW
                     PKGVTEISLSPLSRGGDFNRLFETLRTIVFSLDSTRAYFLEPYSEPFLINNLYVVNTAR
                     QRIIANVPLPGAFDPAVTPDKQYIYAAQPNDNAVTVYRTSDYTAVKVIPVGAGPSAIVM
                     "
     CDS             complement(10971..11456)
                     /locus_tag="ctg35_13"
                     /transl_table=11
                     /translation="MFAYVLNFGLVQKVDLSTGEVTGTIERELIVSSIETDSPERYAYV
                     LEQEFLFNYLTVIDLNTFTISNTQELEPDGQYRMFTSGTGVYLYDDFTGNLYSVSPNGA
                     GVIGNLPQSATDYAFTPNGDFLYATRFIEQSIIVYNTDDYSEETVISLGVSPGAITI"
     CDS             complement(11489..12322)
                     /locus_tag="ctg35_14"
                     /transl_table=11
                     /translation="MRKNKLSFKEKAGAEQEECLCFCEGEASREAMFQAPIELPEGFVV
                     DPAEAVANVTWNADSLSCVSDRCLIQTGPEPDEVGVQFAVRLQGTVTLLVSVSPVRNQY
                     GQGDGAVSVIHNAEIDQVVYYSDEPDDCPDISNITIENLVVVPPFYGSPLSVTGTIVLV
                     PEPEPVYAFTANPNDQSVSVIDTNTHTVVTTIALPYNPAGIEITPDKSAVFVLHPNNNV
                     ISVIDYDTLTVTATILLDQPPRLIRFIPNHEFAYVFTGTAIYVIGIDTLTVERSI"
     CDS             12552..13697
                     /locus_tag="ctg35_15"
                     /transl_table=11
                     /translation="MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEE
                     DQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPRLSDLLLEIDKKQARLTGLLEYYFN
                     FFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFSMDY
                     ARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAVSHFQKAYSMAEAEKQPQLMG
                     RTLYNIGLCKNSQSQYEDAITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKA
                     HEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAIQGFFDFLESKMLYADLEDFT
                     IDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV"
     CDS             13681..13800
                     /locus_tag="ctg35_16"
                     /transl_table=11
                     /translation="MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI"
     CDS             13898..14209
                     /locus_tag="ctg35_17"
                     /transl_table=11
                     /translation="MSLKSVRTHFTQWNRENDVTEFETSSATVEQAAETIGVSLSRIAK
                     SLSFRGEGDQVILIVAAGDAKIDNKKSRQTFGFKARMLSPNEVLEQTGHEIGGVCPIG"
ORIGIN
        1 cctcacagcc gcatgccaga acatgaagaa aattgccaca tacctagcca agcagggcta
       61 ggcatgtggg agcgcttttt ctgcatctga aacctgatga atatctaaag aaactgtata
      121 aaacaaaaaa gcttgtagaa aaaacgtcgt tttctctaca agctaaggac aaagcccgaa
      181 gactttgtcc ttttgatcgt gattacattt tttccggagc cgatacgccg atcagctgaa
      241 gcgcattgtt cagcgtaatt tgcgttgcct tcattaaagc caggcgcgca cggcttttct
      301 cttcattttc agggtcgatg actttctctg cgttgtagaa gctgtgcaga gcagaggcca
      361 gatcataaat atagtttgtg acacggtgcg gaattcgttt ttccgctgct tccgccactg
      421 cctctgggaa gccgccgatc gttttcagca gatcgtattc tttttctgat tgaatatggc
      481 tgaaatcaag atccgccgcc ggtttaagtc cctgctcttc cccttggcga agcatgctgc
      541 agattcgggc atgtgcatat tgtgcataat acacagggtt ttcgttagat gttgatacag
      601 caagatctaa gtcaaaatcc atatgggtgt ccgcacttcg catcgcaaag aagtaacgaa
      661 cggcatccaa gccgacttct tcaatcaggt cacgcatcgt tacggcttta ccggtccgtt
      721 tgctcatttt cattttctcg ccgtttttgt aaaggtgaac gagctgaatg atttctactt
      781 caagcgttcc tttttcatag cccagcgctt cgattgccgc tttcatacgc gggatgtagc
      841 cgtggtgatc ggcgccccaa acgttgatca gcttatcaaa gccgcggtca agcttgtcct
      901 tatgatacgc gatatcagga agcaggtacg tgtacgtgcc gtccttcttg atcaatacgc
      961 ggtctttatc atcgccaaac gtcgtggaac ggaaccaagt cgcgccgtct tcttcataga
     1021 catggccttt ttcgcggagc gcttcaagcg ccttgtcgat ttttccattt tggtacagtg
     1081 aagtttctga ataccacaca tcaaacggca cacggaagtt ttccaagtcc ttgcgaagct
     1141 tttccagctc atatttcagg ccgtattcac ggaaaaacgc gaggcgttca ctttcttctt
     1201 cgttaacgaa acgatcaccg tattcctcag cgagacgctt tccgatcgca atgatatcct
     1261 cgccgcggta gccatcctcc ggcatcggtt tttccaatcc gagtgcttca aagtaacgga
     1321 cttcaacaga aagcgccaga ttgttgattt ggttacccgc atcgttaatg tagtattcgc
     1381 ggcttacatc gtaacccgct tttgaaagca cgttgcacaa ggaatcgccg acagccgccc
     1441 cgcgcgcatg cccgaggtga aggtctcccg tcggattcgc tgatacgaat tctacctgaa
     1501 ctcgttcacc gtttccgatg ttcgtttccc cgtatgcttc ccctgcttcc agtacagacg
     1561 gaatcagctt tgttaaatat tgattgttca tatagaaatt gataaaaccc gggccggcga
     1621 tatccagttt ttcaatcgaa gcctttcctt tatcaaaatg ggcgacaatc tcttcagcga
     1681 tttggcgcgg cgcttttttt gccactcttg ccagctgcat cgccatattc gttgagtagt
     1741 cgccgtgtgt tttatctttc ggtgtttcta aaacaacatt gggaatctgg ctttcttcag
     1801 ccagtcccgc tttcagaacg gccgctttga tttcttcttt cagcacgtcc ttcatttgtt
     1861 ccgcaatgtt catgcgtgag ttcctccctc atacgtgatc gtcatattat gtaaatgctc
     1921 ctgctcgtct ccgacatgca tgtcatatgc gatgctgata cggccttttt cttcatcgag
     1981 atcagattga atcgattttg tgcttgtttt cagctcaagc tcaccaaatg acattttata
     2041 tttagcaatt gtggaagctc cctttacgaa acgctgattc attttgacag cgcctgacct
     2101 catgaccagg acttcgcctt cgcttacttt cacaatcgtt ttgactttgc ctaagtcgtg
     2161 ttcttcataa taagatagat acactttatt ttgttttaca tagtaaaatc cggtggtgcg
     2221 gaattcgatg acttcttggt ttccgtcatc ctcgatcaca gacttcacat ggagtgttat
     2281 cggtgtctct tgcttcattc tgacacgtcc ttttcatcat tccactttga cttgaatagt
     2341 ataatcattc ggtttgtaaa cttcaactgc ttctatctta ccatcattgc tcatcagatt
     2401 tgaagataaa cctcataaaa agcatttcct tatatagaag agaaaatcat atcactaatt
     2461 acctttagta aatgttacat ttttccgaaa tctatcattt ccttttcaca tttttttcaa
     2521 aatatatgta ttgaattagt aatttgatag ttttaagata aaagtacaac atagatctgt
     2581 tagaaaaaca aaaaaaaagg gaggattcaa ttatgaaaaa agctgtcatt gtagaaaaca
     2641 aaggttgtgc aacatgctcg atcggagccg cttgtctagt ggacggtcct atccctgatt
     2701 ttgaaattgc cggtgcaaca ggtctattcg gtctatgggg gtaaggatct cccaaaaggg
     2761 catagtcatt ctactatgcc ctttttaagt aaattacaat ttttaactaa aatctacctc
     2821 ttggatatac atacacaacc gtttttcatt tgaatcatat gtatagggga tggtattttg
     2881 tttatagagc agatgtttcc atttattaat gaaagtgtaa gagttcacca gcttcctgag
     2941 ggcggcgtgt tagaaatcga ctacttgcgc gataatgtct ccatttctga ctttgagtat
     3001 ttggatctca acaaaacagc ttacgagctc tgcatgcgca tggatggcca aaaaacagct
     3061 gagcagattt tagctgagca atgtgcagtg tatgatgaat caccggaaga ccataaagat
     3121 tggtattacg acatgctcaa catgctccag aacaagcagg ttatccagct tggaaaccgg
     3181 gccatccgcc ataccatcac cacgagcgga agcaatgaat ttccgatgcc cctgcacgcc
     3241 acctttgaac tgacgcaccg ctgtaatttg aaatgcgccc actgttattt ggaaagctcg
     3301 cctgaagcgc tcggcaccgt gtcgattgag caattcaaaa aaacggctga tatgctgttt
     3361 gataacggtg tattgacatg cgaaatcaca ggcggagaaa tttttgtcca tccaaacgcc
     3421 aatgagattc ttgactatgt gtgtaaaaag ttcaaaaaag tcgctgtctt aacaaacgga
     3481 acactcatgc gaaaagagag cctggagctt ttgaagactt acaagcaaaa aatcatcgtc
     3541 ggcatttctc ttgatagtgt caattccgag gtccatgact cctttagagg gagaaaaggc
     3601 tcttttgccc aaacttgtaa aacgataaaa ttgttgagtg accacggtat atttgtcaga
     3661 gtcgccatgt ctgtcttcga aaaaaacatg tgggaaatcc acgatatggc ccaaaaggtt
     3721 cgggatctcg gagcgaaggc gttttcttac aattgggttg acgatttcgg aagaggcagg
     3781 gatattatcc atccaacgaa agacgccgag cagcaccgca agtttatgga atacgagcaa
     3841 aatgtgattg atgagtttaa agatctgatt ccgattattc cctatgagag aaaacgcgcg
     3901 gcaaattgcg gcgctggctg gaagtccatt gtgatcagcc cgttcggcga agtacgtcct
     3961 tgcgccctct ttccaaagga attttcattg ggaaatattt ttcatgattc ctatgaaagc
     4021 atctttaact cccctctcgt ccataaactg tggcaagcgc aggcgccgcg gttcagcgaa
     4081 cattgcatga aagacaaatg cccgttcagc ggctattgcg gaggctgtta cttaaaaggg
     4141 ctgaactcta acaaatatca ccgaaaaaac atttgctctt gggcgaaaaa tgaacaatta
     4201 gaagacgtgg tccagcttat ttaggaggga aacgatttgt caccagcaca aagaagaatt
     4261 ttactgtata tcctttcatt tatctttgtc atcggcgcag tcgtctattt tgtcaaaagc
     4321 gattatctgt ttacgctgat tttcattgcc attgccattc tgttcgggat gcgcgcgcgg
     4381 aaggctgact cgcgatgagc attttggata tacacgatgt atccgtttgg tatgaacggg
     4441 acaacgtcat cttagaacaa gtggatttac atttagaaaa aggcgccgtt tacgggttac
     4501 ttggggtaaa cggcgccgga aaaacgacac tgattaatac gctgacaggc gtgaaccgca
     4561 atttcagcgg gcgctttaca ctgtgcggaa ttgaagcgga ggccggcatg ccgcagaaaa
     4621 catcagatca gctgaagact caccgttact tcgccgccga ttatccgctg ctatttacag
     4681 aaattacggc gaaggactat gtgtctttcg tccattcgct ttatcaaaag gatttttcag
     4741 agcagcagtt tgccagtttg gccgaggcct ttcatttttc gaaatacatc aataggagaa
     4801 tttcggagct gtccttgggg aacaggcaaa aggttgtatt gatgacaggc ttattgctgc
     4861 gcgctcccct gtttattttg gatgagccgc tcgtcggttt ggatgtggaa tcgatagagg
     4921 tcttttatca gaaaatgcgc gagtactgtg aagcaggcgg gaccattttg ttttcttctc
     4981 atctgctcga tgtcgtgcaa agattttgtg attatgcggc tattcttcac aacaaacaga
     5041 tccaaaaggt cattccgatt ggagaggaga ccgatctgcg gcgggaattt ttcgaggtga
     5101 tcggccatga ataacataat ccccatcatg tctttgctgt tcaaacagct ttacagccgg
     5161 gaagggaaaa aggacgccat ccgcattgcc gcaggccttg tcattctggc cgtgtttgaa
     5221 atcgggctga tccgccaggc cggcattgat gaatcggtgt tgcgcaaaac gtatatcata
     5281 ctcgcgcttc tcttaatgaa cgcatatatg gtgtttcttt ctgtgacatc tcaatggaag
     5341 gaatcttata tgaaactgag ctgcctgctg ccgatttctt cacggagctt ttggctcgcc
     5401 cagagtgtcg ttttgtttgt cgatacctgt ttgagaagaa ctttattctt ttttatttta
     5461 ccgctgttct tatttggaaa cggaacgctg tcaggggcgc aaacattgtt ttggctcggc
     5521 aggttttcgt tttttaccgt ttactccatt attttcggag ttgtgctaag caaccacttc
     5581 gtcaaaaaga agaacttgat gtttctgctg catgcggcga tattcgcctg tgtatgcctc
     5641 agcgccgctt tgatgccggc cgccacgctt ccgctttgcg cggtgcatat gctgtgggcg
     5701 gtgatcattg actttcctgt ctttctgcag gcgcctccgc agcagggcaa gatacattca
     5761 tttatgcggg gatctgaatt ttcgttttac aaaagagaat ggaaccgatt tatctcttct
     5821 aaagcgatgc tgttaaatta cgcggtaatg gcggcgttca gcggcttctt ctcgttccag
     5881 atgatgaata ccggcatctt caatcagcaa gtgatttata tcgtgatttc cgcgcttttg
     5941 ctcatctgct cgccgatcgc ccttttgtac tcgattgaaa aaaatgaccg gatgctgctc
     6001 atcacgcttc cgatcaagcg gaaaacgatg ttttgggcga aatatcgctt ttattcaggc
     6061 ctattggcag gcggatttct ccttgtcgtg atgattgtgg gtttcatcag cggccgtccg
     6121 atttcagttc tcactttttt gcagtgcatt gagttgctgc tggcgggagc ttttatcagg
     6181 ttaactgcgg atgaaaaaag gccctccttc agctggcaga cggagcagca gctatggagc
     6241 ggtttctcaa aatatcggtc ttatctgttt tgtcttcccc tattcttagc cacattagcc
     6301 ggcaccgctg tatccttggc ggtcattccg atagccggtc tggtcatagt ctattacctg
     6361 caaaaacagg atggtggttt ctttgataca agcaaaagag aacggcttgg aagttaacct
     6421 gctgaagact catcaatttt caactatttc aatcgccgcg agctttctga aaccgatcga
     6481 aagcgcggcg gaacctgagg aagaaacgat ttatttttac ggcgccgccg catacctgaa
     6541 ggagcaaatt attgacgctt tcggttgtgc tgccggcagc cgtttcatgt actcagcgaa
     6601 tctctttttt gatcagcagc tgaaaacctg cgggacccgt ttgattcacc cattatataa
     6661 tgggaatctt catgttgacg ccctgatgaa aacttttgca gacttgtctt tcccttcttc
     6721 tctttcattc gaagcagttg aaaaggccag gaatgagctg ctgctgaaaa tcgaaaagaa
     6781 attcactgat cccttctcct actcggcggc gcgcttggct gaggaggtct tcggaaaccc
     6841 gatgtacggc acaggcatgt ttggccggaa agacaggata aaggcgattc atcctaaacg
     6901 gtttcacgac gcaacggact ttatcgttga tttagtgtcc cagcaaaaac agctgaacat
     6961 attgggacaa gtacaagcat gtgatgttcg gggacatgcc ccgcaaacgt ccgctgtaac
     7021 gtccgaaaga atacccgtaa accggcatgt ttttgaaaca gagacacgca gcgctgcagg
     7081 tccttccgtc ttgacactcg gatttgactg cggagagatg aaagatgcca gcgattatat
     7141 caaaatacag ctaatagacg gattactggg aaagtacggc cattccgctt tattcaagca
     7201 cttcagagag aaagatctgg cagtctatca cgttatcacc cgttacgatg tcatgaacaa
     7261 tttgctgctt gtttcaatat gtacagatca gcttcatgag atagacattc ccccgcgcgt
     7321 tttggaagct gtctcagctt ttcatacaga tgagcgggaa ctagaacagg caaagcagtt
     7381 tttacgcaat gaattgctgc tgcaattcga ttctcccgaa ggtctattgg cctatatggg
     7441 gatcctgcgc cgcttctcct gcacgaaaga agccttgctg gatggcatct cagcggttac
     7501 atgccgtgat gtattgcaat tcatagcgac catcaattat atcggagctc atgtagtaag
     7561 gggatgaaaa gatggaaaag aaagcctttt ttcaacagct ggatgaacga accgatatca
     7621 gatacacaga cagcggacta aaaatattca gattgaaatt cccccgcgcc cacctgcggc
     7681 tgtgcaatgt aaaaatcgat tttggaagcc gggatgtatg tatacgggca gaatccggtg
     7741 atacactgct tccgtatggc acagcgcatt ttttagagca tcttctcttc tggcataacg
     7801 gccgcaatct gtacagtgat ttttttgcgc atggcgcgct tttaaacgca ttcactacct
     7861 acaccgatac caatttcatg tttacctcat taccggatcg gctcagacaa acgatcccca
     7921 tcctgcttga tgctttatgg aaccactcat ttgacaagaa gattgtcgct caggaaaaag
     7981 ctgtgattac aagcgaaatt caaacagcac accttaacca tcagctctca tatcattatc
     8041 agctgatcag catgctttct ccctcctcac cagctgccgt tttccccgca ggcagaatcg
     8101 aagatattga ggcgcttgac atcagtgatt tacaaaaagc ctataaggct gcctaccaaa
     8161 cccatcggat gacgctgttt ttgattgggg gctcagaaaa tacagaaacg ctattgcctc
     8221 ctcacctcca gctggaaaag cgtccggatt atcatgctga aagaaaaatc atcccggcat
     8281 gccctccagc cctctcacaa aaaatgatgc tgggtgatga ggagcggatg gaagatacat
     8341 ggaccggatt acagatcggc gcacttcccg ggcaaaacga tctgttgacg atcaaacttt
     8401 attgggatat cgcggctcgt attctatttc aattagattc accgtttttt caagagattc
     8461 agcaaacgta ccgcctggaa atcgatcgtt tgtcagccga gacttatatc tatgaggatg
     8521 gcggtttctt gatccttcac agtcaaggga cccattcttc agcttatatc gatgtggctt
     8581 catactatgt cacacagaag aaagaacagg ctgccgcctg gcttcaatac ggcaaggact
     8641 ctttgacaga cgcgatcatt tatgacagcg attatgtgcg taaatgcttc gagtgggcag
     8701 ccgagtgtga caggtgcgat tgctcgtttc ttgacatgta ccacatcatt caagatatgg
     8761 atgcgcaggt tttcttgtct ctcattgatg caatggcatc ttctaacaaa gcgatcattc
     8821 acgtttctca gaaagaggcg atcagacaat gaaaaaaaac actgtcttta cccttttatt
     8881 gctgcttata ggtatggcgg cttattcctt cggctgggtt caggccgttg ccgaagccgc
     8941 cgcccactat atacagatga tcaacaatga tgctgtacgg ctcggactgc tggcttgtac
     9001 cgctgccctc ctgatgctgc ctgcttttct ctatcttcac tatgtcaccc aatctgtgaa
     9061 aaacatgaca gcagcttttc agaaactcac acagtctcat caaagctgct gtgactttca
     9121 gcaacacaac ttgtgcagcc gatatgcaga agacgtcaaa tcgctgcgtg acggctataa
     9181 aaacgtccgc caaacatacg tgatggctgc cgtgttgtgc caagtcatca tattcggctg
     9241 catgttcgaa atcgttaaag ccgtcccttt ccgccttcac acgccccctg ccctttcaat
     9301 gggacttgcg atgctactga tcctgtattt attgttttgc atgcgaacgt atttgcggca
     9361 gcttttccgg cacggcagcc tgttcagaaa agtgtttgcg ggagtgctgg cagcagccgg
     9421 gatttggtgg atgctcagtt tcactataag tgaactgctg tttctgatta tcctggctgc
     9481 catccagcaa atcagatcat ttatttataa gcgattctct tatcgcagca ccgcttctct
     9541 tgatctataa aatagctaca tcacaatcgc agacgggccc gcgccaaccg gaatcacctt
     9601 cacggctgta taatcacttg tgcggtacac agtcacagca ttgtcgttgg gctgtgccgc
     9661 ataaatatac tgtttgtccg gcgttacggc ggggtcaaaa gcgccgggga gaggcacgtt
     9721 tgcaatgatc ctctgtctgg ccgtattaac tacgtataag ttattaatga gaaacggttc
     9781 actatatggc tctagaaaat aagctctagt actatcgaga gaaaatacaa tcgtcctcag
     9841 cgtttcaaat aaacggttaa agtcccctcc cctgcttaat ggtgacaaac taatctccgt
     9901 tacacctttc ggccacagct caccattcaa cacgtacgca aaatttccag cgtaaggagt
     9961 tgcaattaaa ctgggaaatt caaatgaatc tatgatactt tctaccaact gcccagtatt
    10021 tacatcatat ttatcaaccg accaactgct tccattagcg gtatagacat actgtccatt
    10081 cggatcaaga gcaaagtcta aagcgccggg tatcgcgatt acatcaatta ctgattgatt
    10141 ggccgtgtca atcatatata tagatccggc ggccatcacg taggcgtact catctgttgg
    10201 atcaaattcg atctttctcg gtcctcctcc aacagtaatg gtatttatga tggttaatgt
    10261 tttattatca atcactgaga caaggttggt attcccatgg aggacatacg tgaatgcttt
    10321 atcgaaagta acgcctagat tagttggcac agcagaaaac aggattgttt ttacaacagt
    10381 gtttaaatct gcatcaatga ccgaaaccgt gctgtctcca gtattggcag taaacacata
    10441 cgattgaggg tctggcgatg gcggcaaaac aagtgtcccg gccaccgtga gcggactgcc
    10501 gtagaaaggc ggcacgatga gaagatcttc cactgtcatc tgactaaaat caggacagcg
    10561 gtcagactgt gctgcataat aaacaacttg atcaatctct tcagtatgaa tcacagaaac
    10621 cgccccgtct ccttgtccat attgatttcg cacaggcgac acactgacaa gaagcataat
    10681 ggtcccttgt actctcacag catatcgaac accaatatct tctgtttccg gcccagtctg
    10741 gattaagcaa ggctcactga cacaggacaa attgtctgta ctccatgtaa cagccgctga
    10801 cgcttcggct gaatcaacct tgaagccctt agggatatct attggcactt gaaacggcgc
    10861 ttcccggcta atttcctcat cacaaaaaca gaaacaattt tcctgtaaag cagaaagatc
    10921 attctttttc atgaattcac gtccttttaa aaatgcacaa catgcagcgg ttaaatcgta
    10981 atggcacccg gtgaaacccc aagagatatc acagtttcct cagaataatc atctgtgttg
    11041 taaacaatga tgctctgctc tataaaacga gttgcataca gaaaatcgcc attcggggta
    11101 aacgcatagt ctgttgctga ttgcggaaga tttccaatta cacctgctcc attcggactg
    11161 acagaatata aattgccggt aaagtcgtca tataaataca cacctgttcc gctcgtaaac
    11221 attctatatt ggccgtcagg ctccagttct tgggtattgc tgatcgtaaa cgtatttaag
    11281 tcaataaccg tcaaataatt gaataagaat tcttgttcta agacatacgc ataccgctcc
    11341 ggagaatctg tttctatgga tgatacgata agctctcgtt caatcgttcc tgtgacttca
    11401 ccggtggata agtccacttt ttgcactaac ccaaaattaa gaacataggc gaacaaccca
    11461 ttggggtcaa ttgcaatatc aaaaccttct atatagacct ctcaacagtc aacgtatcaa
    11521 ttccaatcac ataaatggca gtaccggtga aaacataagc aaactcatga ttagggataa
    11581 atctaatcaa tcgaggcggc tgatccagca atatagttgc tgtcactgtt aatgtgtcat
    11641 aatcgatgac agaaatcaca ttattgttgg gatgtaagac aaacacagcg cttttatctg
    11701 gcgtaatttc aatacctgcc ggattgtacg gaagagcaat cgttgtcaca acagtatggg
    11761 tgtttgtatc aatcacagaa actgattgat catttggatt cgctgtaaac gcgtagacgg
    11821 gttccggctc cggtacaagc acaatcgtcc ccgtcaccga taaaggactg ccgtaaaacg
    11881 gcggaacaac aacaaggttc tcaatcgtga tgttactgat atccgggcaa tcatccggtt
    11941 catcggaata atagacgact tgatcgattt ctgcattgtg aatgacggaa acagccccat
    12001 ccccttgtcc atattgattt cgcacaggtg agacactgac gagcagcgta acggttccct
    12061 gcagacgcac tgcaaattga acaccgacct catcaggctc tggccccgtt tgaattaagc
    12121 aacggtcact gacacaagaa aggctgtctg cattccacgt cacgtttgca acagcttcag
    12181 ccggatcaac gacaaatcct tcaggtaatt caatcggcgc ttgaaacatg gcttctcggc
    12241 ttgcttctcc ctcacaaaaa cataagcact cttcttgttc tgctcctgct ttttctttaa
    12301 aggaaagctt gttttttctc attccttcac ttccttttca gacatacact gctcacgcag
    12361 atataacaga atatggaaga gtaacgtgtt tgcttgtaca aattactgag ggtaaaagct
    12421 tctttttgaa aatatatctc caaacatgcg gtgtaacgaa agaaaaaatt gcggtttttt
    12481 tgcgcttctc acaagaatgt tgatgataaa atatgacata tatagaatta tgaagggagg
    12541 gatttgcaaa cgtgacaggt gtcatatctt cttcttccat cggagaaaag attaacgaat
    12601 ggtatatgta catacgccga ttcagcatac ccgatgcaga atatttgcga cgagaaatca
    12661 agcaagagct ggatcaaatg gaagaagatc aagaccttca tttgtactat tcactgatgg
    12721 agtttcggca caacctgatg cttgagtacc ttgaaccgtt agaaaaaatg aggattgagg
    12781 aacagccgag actgtctgat ctgctgcttg agattgataa aaaacaggct cgtttaacag
    12841 gtctgcttga gtactatttt aatttcttca gaggcatgta tgagctggat cagcgggaat
    12901 atctgtcggc tattaaattt ttcaaaaagg ccgaaagcaa gctgatattc gttaaggatc
    12961 ggatagagaa agctgagttt ttctttaaga tgtctgaatc ttattactat atgaaacaaa
    13021 cgtatttttc aatggactat gcacggcaag catatgaaat atacaaagaa aatgaagctt
    13081 ataatataag attgctgcag tgtcattctt tatttgccac caatttttta gatttaaaac
    13141 agtatgagga tgccgtctca cattttcaaa aagcttattc tatggcagaa gctgaaaagc
    13201 agccccaatt aatgggaaga actttgtaca atatcgggct ttgtaaaaac agccaaagcc
    13261 aatatgagga tgccataaca tatttcaaaa gagcaatagc tgtttttgaa gaatcaaata
    13321 ttcttccttc cttacctcaa gcgtattttt taattacaca gatccattat aaattaggaa
    13381 aaatggataa agctcatgaa tatcatagta agggaatggc ttattcacaa aaggccggag
    13441 atgtaatata tttatcagag tttgaatttt tgaaatcttt atacttatca ggcccggatg
    13501 aagaagcaat tcaaggattt tttgattttc tcgaaagtaa aatgttgtat gctgatcttg
    13561 aagatttcac tattgatgtg gcaaaatatt atcatgaacg taaaaatttt caaaaagctt
    13621 ctgcttattt tttgaaggtg gaacaagtaa ggcaacttat tcaaggagga gtgagtttgt
    13681 atgaaattga agtctaaact attactctct tgtctggctc taagcactgt gttcgtggca
    13741 acaactattg caaatgcacc tacacaccaa attgaagttg cacaacgagg aatgatttaa
    13801 ccgccgtcca tcggcggttt ttttgtcccc tctttaccca gtctcccaat ccattctatg
    13861 attttttcaa taatcttaaa aatagtggaa atgcagcatg tctctaaaaa gtgtgagaac
    13921 ccactttact caatggaatc gagaaaatga tgtgacggag ttcgaaacgt cgagtgcgac
    13981 agttgaacag gcagctgaga caatcggcgt aagcctgtct agaatcgcca agtccctgtc
    14041 cttcagaggg gaaggagatc aggtgattct gattgtggca gccggcgatg ccaagatcga
    14101 caacaaaaag tccaggcaaa catttggctt taaagcaaga atgctctctc ctaatgaggt
    14161 actggagcag acaggccatg aaattggagg agtttgccca attggataga ctcatgatcc
    14221 tgag
//
