LOCUS       gnlProkkaPOJNMFHH_20    1314 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|POJNMFHH_20.
ACCESSION   gnlProkkaPOJNMFHH_20
VERSION     gnlProkkaPOJNMFHH_20
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 19:43:06
            Original ID  :: gnl|Prokka|POJNMFHH_20
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 1314
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..1314
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:1314]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..1314
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..1314
                     /SMILES="NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..1314
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /tool="antismash"
     CDS             1..1314
                     /NRPS_PKS="Domain: AMP-binding (1-237). E-value: 1.1e-56.
                     Score: 183.9. Matches aSDomain:
                     nrpspksdomains_ctg20_1_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (342-410). E-value: 6.3e-28. Score:
                     88.7. Matches aSDomain: nrpspksdomains_ctg20_1_PCP.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     202.6; E-value: 1.4e-61)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg20_1"
                     /sec_met_domain="AMP-binding (E-value: 4.2e-74, bitscore:
                     235.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 8.7e-20, bitscore:
                     57.4, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGQPKGVMVEHHALVNLCFWHHDAFAMTADDKSAKYAGFGFDASI
                     WEMFPTWTIGAELHIIDEAIRLDITRLNHYFQEHGVTITFLPTQLAEQFMELENSSLRM
                     LLVGGDKLKRAVKQPYTIVNNYGPTENTVVATSGVINPEEDSLSIGRAIANTRAYILGD
                     GDQVQPEGIAGELCVAGRGLARGYLNREEETAKRFTADPFVPGERMYRTGDLVKWNTQC
                     GIEYIGRIDQQVKVRGYRIELSEIEVRLAQLAGVHDAAVTAVEDKAGNTALCAYVAPQQ
                     TDIEALKAALKDTLPDYMVPAFWVEMDELPVTANGKIDKKALPSPDIEAGSAAYKAPET
                     EMETLLSDIWQEVLGLEQIGVSDNFFTLGGDSIKGIQMASRLNQHGYKLEMKDLFQHPT
                     IEELVSYVERTEGKQADQGPVEGEADLTPIQRWFFEKNF"
     aSDomain        4..711
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg20_1_AMP-binding.1"
                     /evalue="1.10E-56"
                     /label="ctg20_1_AMP-binding.1"
                     /locus_tag="ctg20_1"
                     /protein_end="237"
                     /protein_start="1"
                     /score="183.9"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="GQPKGVMVEHHALVNLCFWHHDAFAMTADDKSAKYAGFGFDASIW
                     EMFPTWTIGAELHIIDEAIRLDITRLNHYFQEHGVTITFLPTQLAEQFMELENSSLRML
                     LVGGDKLKRAVKQPYTIVNNYGPTENTVVATSGVINPEEDSLSIGRAIANTRAYILGDG
                     DQVQPEGIAGELCVAGRGLARGYLNREEETAKRFTADPFVPGERMYRTGDLVKWNTQCG
                     IEYIGRIDQQVKVR"
     aSModule        4..1230
                     /complete
                     /domains="nrpspksdomains_ctg20_1_AMP-binding.1"
                     /domains="nrpspksdomains_ctg20_1_PCP.1"
                     /locus_tags="ctg20_1"
                     /monomer_pairings="Leu -> Leu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       373..402
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg20_1_0001"
                     /evalue="2.10E-03"
                     /label="NRPS-A_a5"
                     /locus_tag="ctg20_1"
                     /protein_end="134"
                     /protein_start="124"
                     /score="10.6"
                     /tool="antismash"
                     /translation="NYGPTENTVV"
     CDS_motif       505..594
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg20_1_0002"
                     /evalue="6.70E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg20_1"
                     /protein_end="198"
                     /protein_start="168"
                     /score="50.4"
                     /tool="antismash"
                     /translation="PEGIAGELCVAGRGLARGYLNREEETAKRF"
     CDS_motif       676..741
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg20_1_0003"
                     /evalue="7.90E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg20_1"
                     /protein_end="247"
                     /protein_start="225"
                     /score="37.1"
                     /tool="antismash"
                     /translation="YIGRIDQQVKVRGYRIELSEIE"
     aSDomain        1027..1230
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg20_1_PCP.1"
                     /evalue="6.30E-28"
                     /label="ctg20_1_PCP.1"
                     /locus_tag="ctg20_1"
                     /protein_end="410"
                     /protein_start="342"
                     /score="88.7"
                     /tool="antismash"
                     /translation="ETLLSDIWQEVLGLEQIGVSDNFFTLGGDSIKGIQMASRLNQHGY
                     KLEMKDLFQHPTIEELVSYVERT"
     CDS_motif       1090..1119
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg20_1_0004"
                     /evalue="5.40E-03"
                     /label="PCP_mC"
                     /locus_tag="ctg20_1"
                     /protein_end="373"
                     /protein_start="363"
                     /score="10.2"
                     /tool="antismash"
                     /translation="NFFTLGGDSI"
     CDS_motif       1276..1305
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg20_1_0005"
                     /evalue="2.50E-05"
                     /label="NRPS-E1"
                     /locus_tag="ctg20_1"
                     /protein_end="435"
                     /protein_start="425"
                     /score="16.8"
                     /tool="antismash"
                     /translation="LTPIQRWFFE"
ORIGIN
        1 accggacagc cgaagggtgt catggtcgaa caccatgcgc tcgtgaatct gtgcttctgg
       61 caccacgacg cattcgccat gacggcggac gataagagcg ccaaatacgc gggcttcggt
      121 tttgacgcct ccatctggga gatgttcccg acatggacca tcggcgcgga gcttcacatc
      181 attgacgaag cgatccggct ggatatcacc cgcttaaatc actatttcca agagcacggc
      241 gtgacgatca ccttcctgcc gacgcagctg gccgaacagt ttatggagct ggaaaatagc
      301 tctctccgca tgcttctcgt cggaggcgac aagctgaagc gggcggtgaa acagccgtac
      361 acgatcgtca acaactacgg cccgacggaa aacaccgtcg tcgcaacaag cggcgtgatc
      421 aatccggagg aagattcgct ttcaatcgga cgggcgattg ccaatacgag agcttatatt
      481 ctcggcgacg gcgatcaggt gcagccggaa ggcattgccg gtgaattgtg cgtggcaggc
      541 cgcgggcttg cgcgcggata cctgaaccgt gaagaagaaa cggcgaagcg gtttaccgcc
      601 gatccgtttg tgcccggcga gcgcatgtac cggaccggcg acctcgtcaa atggaacacg
      661 cagtgcggca tcgaatacat cggccgcatc gaccagcagg tcaaagtgcg cggctaccgg
      721 atcgagcttt cagaaattga agtccgcctc gcccagcttg cgggagttca tgacgcagcc
      781 gtgacagcgg tggaagacaa agcgggcaat accgcgcttt gcgcctatgt cgcgcctcag
      841 cagacggaca tcgaagcgct caaagcggcg ttgaaagata cccttcctga ctacatggtg
      901 ccggcgttct gggtggagat ggacgagctt ccggtcaccg caaacggaaa gattgacaaa
      961 aaagccctgc cgtcgccgga cattgaagcg ggaagcgccg cgtacaaagc gccggaaacg
     1021 gaaatggaga cgctgctttc tgacatttgg caggaagtgc tcggccttga acagatcggc
     1081 gtaagcgata atttcttcac gctcggcggc gactcgatca aaggcatcca aatggcgagc
     1141 cgcctgaacc agcacggcta caagctggag atgaaggatc tcttccagca cccgaccatc
     1201 gaagaactcg tctcctatgt ggagcggacg gaaggcaagc aggccgacca gggccctgtc
     1261 gaaggcgaag cggacttaac gccgatccag cgctggttct ttgagaaaaa cttt
//
