LOCUS       gnlProkkaBNGFGAPD_98    9676 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BNGFGAPD_98.
ACCESSION   gnlProkkaBNGFGAPD_98
VERSION     gnlProkkaBNGFGAPD_98
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 20:10:52
            Original ID  :: gnl|Prokka|BNGFGAPD_98
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 9676
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..3309
                     /NRPS_PKS="Domain: Heterocyclization (58-356). E-value:
                     4.1e-84. Score: 274.3. Matches aSDomain:
                     nrpspksdomains_ctg95_1_Heterocyclization.1"
                     /NRPS_PKS="Domain: AMP-binding (525-923). E-value: 1.2e-76.
                     Score: 249.7. Matches aSDomain:
                     nrpspksdomains_ctg95_1_AMP-binding.1"
                     /NRPS_PKS="Domain: PP-binding (1038-1096). E-value:
                     5.6e-10. Score: 31.5. Matches aSDomain:
                     nrpspksdomains_ctg95_1_PP-binding.1"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127:condensation domain-containing protein (Score:
                     167.9; E-value: 7e-51)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg95_1"
                     /sec_met_domain="Condensation (E-value: 4.8e-48, bitscore:
                     152.6, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 3.3e-89, bitscore:
                     288.1, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 7e-11, bitscore:
                     31.5, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLRAMRMMGELSCKYGIHIEMSPFLENPVLSNLICLANASLNVD
                     LPAFEHLPTDQQLEPFELNESQQALWIGRGSDFNYGGVGCQGYFEWEVENLDHDRFVRA
                     VGLLVDRHPMLRMTIDDAGRQRINTVDGRQAVEYIDLRELSPVEISNEINQLRRRMAND
                     EIGTTQWPLFRFVVSRISTHLSRIHLCIDMLIADAWSIFQVIIPDLIDFYINEKSQLPQ
                     LQTTFHDYVAYRNKLRQSAQYLAHREYWLEKIKELPAAPKLPQLEPGETTAPVRFERHE
                     GKLEQESWARLKAQAQERRISPSGVVALVLCEVLRCWSEEDWFTLNFPVSDRMPVSDDI
                     DLVVGDFTNTLLVPYETEVEDTFEARGRRLQDAIWEALDHRLFTGVEVLRELARIRRSG
                     REPLMPVVLTSLLGHPGRHDVSLLGREVFGVSQTPQVTLDVQVRESEGTLYFKWDYLTG
                     VIRPDVIEAMFGAFSNLLQQLTDDSEVWKRTWLDLRPASQIAVRNEVNATEEPVPEVHL
                     RDLLLERLRDSANEPAVIDIHGEYTWDEIGRAATHVKMMIQRVCNSSDRFVGIMLPKGV
                     AQYEAVYGCLLAGIGYVPIDIDLPQERVRTILAQAGVRIVIASPDTMVPVGVHKIEHVT
                     AGPGSWMQQNTDFVIEPVSEDYSPYVIFTSGSTGEPKGVEIPEAAVVNHIFDVVERFGL
                     DKSIRHLATAALHFDMSVFDVFGPLVHGGSVVVPEPAAGPTPEAWLRLHRQCRVTFWAC
                     VPAVMDLVCSVAETALTVHPIESVTNIVMAGDWIPLSLLPRARSVFPSARMYSCGGPTE
                     TTNWSVIHEINEDEGSICRSVIYGSPMRNSKYHIVSENWIDRPDWVPGEMLVESDISLA
                     RGYLGKPELTQCVFVYHPRTGRQMYRTGDLGRYLPNGEIEILGRVDNQIKIKGIRIELG
                     EIERAAEACDGVSRACAVALPGQDGRPKQIGLAYIGESNLDDAIIKELTHRLPNYMVPK
                     TIKKVPNLPLSKNGKVDVRALRERLLKTDTKELNTGDRRTILREVIRVISVQLAQPVIL
                     PEDNFIQLGGDSLTAMNLKIELESKLSITVSLESIMLTETIGEFANELAERMKL"
     protocluster    1..9676
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:3309]"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..3309
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     cand_cluster    1..9676
                     /SMILES="NC(CS)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..9676
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX))"
                     /tool="antismash"
     aSDomain        175..1068
                     /aSDomain="Heterocyclization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg95_1_Heterocyclization.1"
                     /evalue="4.10E-84"
                     /label="ctg95_1_Heterocyclization.1"
                     /locus_tag="ctg95_1"
                     /protein_end="356"
                     /protein_start="58"
                     /score="274.3"
                     /tool="antismash"
                     /translation="EPFELNESQQALWIGRGSDFNYGGVGCQGYFEWEVENLDHDRFVR
                     AVGLLVDRHPMLRMTIDDAGRQRINTVDGRQAVEYIDLRELSPVEISNEINQLRRRMAN
                     DEIGTTQWPLFRFVVSRISTHLSRIHLCIDMLIADAWSIFQVIIPDLIDFYINEKSQLP
                     QLQTTFHDYVAYRNKLRQSAQYLAHREYWLEKIKELPAAPKLPQLEPGETTAPVRFERH
                     EGKLEQESWARLKAQAQERRISPSGVVALVLCEVLRCWSEEDWFTLNFPVSDRMPVSDD
                     IDLVVGDFTNTLLVPYE"
     aSModule        175..3288
                     /complete
                     /domains="nrpspksdomains_ctg95_1_Heterocyclization.1"
                     /domains="nrpspksdomains_ctg95_1_AMP-binding.1"
                     /domains="nrpspksdomains_ctg95_1_PP-binding.1"
                     /locus_tags="ctg95_1"
                     /monomer_pairings="Cys -> Cys"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       322..375
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg95_1_0001"
                     /evalue="2.30E-08"
                     /label="Cy2"
                     /locus_tag="ctg95_1"
                     /protein_end="125"
                     /protein_start="107"
                     /score="26.2"
                     /tool="antismash"
                     /translation="LVDRHPMLRMTIDDAGRQ"
     CDS_motif       556..600
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg95_1_0002"
                     /evalue="8.70E-07"
                     /label="Cy3"
                     /locus_tag="ctg95_1"
                     /protein_end="200"
                     /protein_start="185"
                     /score="21.9"
                     /tool="antismash"
                     /translation="RIHLCIDMLIADAWS"
     CDS_motif       898..957
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg95_1_0003"
                     /evalue="1.30E-04"
                     /label="Cy4"
                     /locus_tag="ctg95_1"
                     /protein_end="319"
                     /protein_start="299"
                     /score="14.9"
                     /tool="antismash"
                     /translation="RISPSGVVALVLCEVLRCWS"
     CDS_motif       1012..1065
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg95_1_0004"
                     /evalue="1.60E-06"
                     /label="Cy5"
                     /locus_tag="ctg95_1"
                     /protein_end="355"
                     /protein_start="337"
                     /score="20.4"
                     /tool="antismash"
                     /translation="DDIDLVVGDFTNTLLVPY"
     CDS_motif       1129..1176
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg95_1_0005"
                     /evalue="2.90E-06"
                     /label="afterCy5"
                     /locus_tag="ctg95_1"
                     /protein_end="392"
                     /protein_start="376"
                     /score="19.8"
                     /tool="antismash"
                     /translation="ALDHRLFTGVEVLREL"
     CDS_motif       1288..1335
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg95_1_0006"
                     /evalue="9.60E-06"
                     /label="Cy7"
                     /locus_tag="ctg95_1"
                     /protein_end="445"
                     /protein_start="429"
                     /score="18.5"
                     /tool="antismash"
                     /translation="VSQTPQVTLDVQVRES"
     CDS_motif       1393..1428
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg95_1_0007"
                     /evalue="4.80E+01"
                     /label="Cy5"
                     /locus_tag="ctg95_1"
                     /protein_end="476"
                     /protein_start="464"
                     /score="-2.9"
                     /tool="antismash"
                     /translation="IEAMFGAFSNLL"
     aSDomain        1576..2769
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg95_1_AMP-binding.1"
                     /evalue="1.20E-76"
                     /label="ctg95_1_AMP-binding.1"
                     /locus_tag="ctg95_1"
                     /protein_end="923"
                     /protein_start="525"
                     /score="249.7"
                     /specificity="substrate consensus: Cys"
                     /tool="antismash"
                     /translation="RDSANEPAVIDIHGEYTWDEIGRAATHVKMMIQRVCNSSDRFVGI
                     MLPKGVAQYEAVYGCLLAGIGYVPIDIDLPQERVRTILAQAGVRIVIASPDTMVPVGVH
                     KIEHVTAGPGSWMQQNTDFVIEPVSEDYSPYVIFTSGSTGEPKGVEIPEAAVVNHIFDV
                     VERFGLDKSIRHLATAALHFDMSVFDVFGPLVHGGSVVVPEPAAGPTPEAWLRLHRQCR
                     VTFWACVPAVMDLVCSVAETALTVHPIESVTNIVMAGDWIPLSLLPRARSVFPSARMYS
                     CGGPTETTNWSVIHEINEDEGSICRSVIYGSPMRNSKYHIVSENWIDRPDWVPGEMLVE
                     SDISLARGYLGKPELTQCVFVYHPRTGRQMYRTGDLGRYLPNGEIEILGRVDNQIKIK"
     CDS_motif       1978..2028
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg95_1_0008"
                     /evalue="4.60E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg95_1"
                     /protein_end="676"
                     /protein_start="659"
                     /score="25.3"
                     /tool="antismash"
                     /translation="YVIFTSGSTGEPKGVEI"
     CDS_motif       2119..2145
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg95_1_0009"
                     /evalue="3.50E+01"
                     /label="Cy3"
                     /locus_tag="ctg95_1"
                     /protein_end="715"
                     /protein_start="706"
                     /score="-1.5"
                     /tool="antismash"
                     /translation="HFDMSVFDV"
     CDS_motif       2563..2655
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg95_1_0010"
                     /evalue="2.30E-07"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg95_1"
                     /protein_end="885"
                     /protein_start="854"
                     /score="23.2"
                     /tool="antismash"
                     /translation="PDWVPGEMLVESDISLARGYLGKPELTQCVF"
     CDS_motif       2737..2799
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg95_1_0011"
                     /evalue="1.40E-09"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg95_1"
                     /protein_end="933"
                     /protein_start="912"
                     /score="30.1"
                     /tool="antismash"
                     /translation="LGRVDNQIKIKGIRIELGEIE"
     CDS_motif       2836..2880
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg95_1_0012"
                     /evalue="3.80E+01"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg95_1"
                     /protein_end="960"
                     /protein_start="945"
                     /score="-1.7"
                     /tool="antismash"
                     /translation="CAVALPGQDGRPKQI"
     CDS_motif       3013..3054
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg95_1_0013"
                     /evalue="5.30E+01"
                     /label="Cy7"
                     /locus_tag="ctg95_1"
                     /protein_end="1018"
                     /protein_start="1004"
                     /score="-2.3"
                     /tool="antismash"
                     /translation="VDVRALRERLLKTD"
     aSDomain        3115..3288
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg95_1_PP-binding.1"
                     /evalue="5.60E-10"
                     /label="ctg95_1_PP-binding.1"
                     /locus_tag="ctg95_1"
                     /protein_end="1096"
                     /protein_start="1038"
                     /score="31.5"
                     /tool="antismash"
                     /translation="SVQLAQPVILPEDNFIQLGGDSLTAMNLKIELESKLSITVSLESI
                     MLTETIGEFANEL"
     CDS             3306..4628
                     /locus_tag="ctg95_2"
                     /transl_table=11
                     /translation="MNKKQLYLEWPEYVEAYLNGQTKLQGLHEKVELRGQEILEYVEVQ
                     SPFYREHLRTQVGFTSEFKVLDGIPFTTKSDLRSAGTEVCCVPMDEIAAYYETTGTTGK
                     STPCPRASIDVETSGAYVQNAVKEVYQSTFGTMHALTAIMGPSELYAFGDTYGEVCRNL
                     GIPFVRLWPESPRVGLEKAADLITGLGVKSLICSPAVALALARFYKSLGIDPCKTPVSQ
                     ILVLGELCTPEMLSNISRIWGANCTHGLYGSQETHAVATGCSEGNLHLSETNYIVETIP
                     INGLDGDVGELCLTMLVPGAKPLIRFRTGDLASLYPSSVCGCGNPSRCIRIFGRVDDVM
                     TIGGCQMFPASIESAVLRAADRVWGYQVDIRISNNGTDELEVAMVAEVGNQSFDQIQKE
                     IADHFGVPVRLVLQDCLDPRTETGAYISWKHARIRDWRPTL"
     CDS             4625..6025
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1018:isochorismate synthase (Score: 266.7; E-value:
                     8.2e-81)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg95_3"
                     /transl_table=11
                     /translation="MNTQEQKDSWVVEQSVVPEAAPVAQVRRRVLSLYPRSALFETGTG
                     FQAVDGKQTMFMVDLLFELILVDGTWQVFGSGPFEMKTSECSEVVNDHTKPLWERMRKV
                     TQILALPEGFPLVLVLGYSAARFVEHLPRIAIEKGEPEVVLRVYRHVVRYNGAEGGAQV
                     DVLKCRHDEVTDTEILIATLTDPEIDLAPRGPWDLGVIQGLTERSTFCDAVNRAKEHIR
                     AGDIYQVQLCRCAISSARIPPLDLYERLASINPAPYMYYLDLGDRHVVSSSPELMIRSG
                     DGGAQVRPIAGTMAQEDQRGSRLDHIPKEAAEHLMLVDLARNDLARCAIPGGVDVTSFM
                     QMDAYGPLFHLVSTVETPVRADSDIWDLIEANFPAGTMTGAPKVRAMEIIAELEDTARG
                     LFTGCAGYINGENSGVLALTIRTIVGDSGRYVLRAAAGIVADSKASAEWDEAGAKIRSF
                     ARAIGGSV"
     CDS             6022..6615
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1249:carbamoyl phosphate synthase small subunit
                     (Score: 126.6; E-value: 1.7e-38)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg95_4"
                     /transl_table=11
                     /translation="MRVLLVDAFDSFVFVIAQYYAKVGAHTKVVRVNDDPISQYQRWKP
                     QLLVLGPGPGTPQEHGYPEIIRAAGEHQAIFGVCLGHQAIGEFFGCKLKNAPTIEHGKK
                     AQVQHDGQGIFCHVPSPVTVVRYHSLAITDPGTENELVTSAFSQEDGVVMGVRHRTRPI
                     EGVQFHPESIGTEHGITMIRNSLALANYPSLMSS"
     CDS             6799..8247
                     /gene_functions="transport (smcogs) SMCOG1005:Drug
                     resistance transporter, EmrB/QacA (Score: 291.9; E-value:
                     1.1e-88)"
                     /gene_kind="transport"
                     /locus_tag="ctg95_5"
                     /transl_table=11
                     /translation="MIQTSKNEIEVISEVRWKALAVISIIQFILLLDATVVNVALPHIK
                     QDLGFTAASLTWVVNAYLVAAGSLLLLGGRIGDAFGLRRTFQIGIAVFGVFSLVATLSS
                     NASMLIVGRAGQGVGEALTAATGLAMVSLLFPSGPERGKAFSIWAALGGFGSIVGVMLS
                     GVLTEYLSWRWVFGINIPIILILYVTVVLLVPKLSNQATARLDIGNALVLAFSVSVIVL
                     GIIGSGIEQLLWLRVTLSLLGMLGIIGVLQRCKRSGDGIIPARLMARSPRIIGYAIVAI
                     LAGTSGALFYLGVLLLQDSLGMTPMQAGMAWLPFCLGFFPGLFLFQFIIKRWSSRAAAL
                     VGLTVSALGFLLFAVGVTTQSYWVGMMPAMIVTSVGFGCVSPVSQYLATFGLSEVDAGA
                     GSGITSTIQQLFNVGGVTLLAAVAISITDGTGASTIINPKGFIVSFVLAAVAIICGAVL
                     ILVRGSALASSPDNQESLTDGRES"
     CDS             8569..9402
                     /NRPS_PKS="Domain: Condensation_LCL (24-266). E-value:
                     3e-11. Score: 34.7. Matches aSDomain:
                     nrpspksdomains_ctg95_6_Condensation_LCL.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127:condensation domain-containing protein (Score:
                     83.3; E-value: 3.4e-25)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg95_6"
                     /sec_met_domain="Condensation (E-value: 4.4e-09, bitscore:
                     24.6, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQQKYLVPPVGRQLITVPSTAGLREKWVGAMLDKTGRAYAGALRL
                     DFSPSCESDKALNAVADTLARHPILAAHFQIEGDILVGYAPTESSLREVLREQMRTKHT
                     GITNEAFLAHRARQSSEPGLRLASVSDDNGLHVWIGFWTYTCDGASIDLLIEEIANRYL
                     GKSLKSSRSWEEYAQMEVTQTVKQVLDSRTRVNVYPVPGPYGIDAVRATPRGTVGHTQS
                     VRFDSPIPRSMVLTCARAYRVTPFVLLFAAFQRAVSAVSGVKTVVRSPIRKPSAS"
     aSDomain        8641..9366
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg95_6_Condensation_LCL.1"
                     /domain_subtypes="Condensation_LCL"
                     /evalue="3.00E-11"
                     /label="ctg95_6_Condensation_LCL.1"
                     /locus_tag="ctg95_6"
                     /protein_end="266"
                     /protein_start="24"
                     /score="34.7"
                     /tool="antismash"
                     /translation="EKWVGAMLDKTGRAYAGALRLDFSPSCESDKALNAVADTLARHPI
                     LAAHFQIEGDILVGYAPTESSLREVLREQMRTKHTGITNEAFLAHRARQSSEPGLRLAS
                     VSDDNGLHVWIGFWTYTCDGASIDLLIEEIANRYLGKSLKSSRSWEEYAQMEVTQTVKQ
                     VLDSRTRVNVYPVPGPYGIDAVRATPRGTVGHTQSVRFDSPIPRSMVLTCARAYRVTPF
                     VLLFAAFQRAVSAVSGVKTV"
     CDS             9365..9676
                     /locus_tag="ctg95_7"
                     /transl_table=11
                     /translation="MLGVPFANRRHPDDHTVVGPLSNTVPITTHHELGEPLVAVLNQVQ
                     RAVIRAAERQNIETTALYPEGTSPRNVDYDLPFPQLFNAWNSQRDGTEISLGKTEWMYL
                     "
ORIGIN
        1 gactctttgc gggctatgcg catgatgggt gaattatctt gcaagtatgg tatccacatt
       61 gagatgagtc cttttcttga gaacccggta ttatcaaacc ttatatgctt ggctaatgct
      121 tctctcaatg tcgatttgcc agcatttgag cacctgccaa ctgatcaaca attagagccc
      181 ttcgagctaa atgaaagcca acaggcttta tggattggtc gcggctctga cttcaactac
      241 ggcggtgtcg ggtgtcaagg atactttgag tgggaagtgg agaatttaga tcatgatcgg
      301 tttgtgcgcg cggtcggctt gttggtagac cgccatccaa tgcttaggat gacaattgat
      361 gatgctggtc gccaaagaat caatacagtc gatggaaggc aggccgttga gtatatagat
      421 ctaagagagc tttcaccggt cgagattagc aacgagatta accagttgcg tcgacgtatg
      481 gcaaacgacg aaatcgggac aacgcagtgg cccctattcc gatttgtcgt gagccggatt
      541 agcacgcatt taagcaggat tcatttgtgc attgacatgc tgatcgccga tgcatggtcg
      601 atcttccaag taatcattcc tgatttgatt gacttttata ttaatgaaaa gtcacaactg
      661 ccgcagctcc agaccacatt ccacgattat gtcgcgtatc ggaacaagct aaggcagagt
      721 gcacaatacc ttgcgcaccg cgagtattgg ttagaaaaaa tcaaggagtt gccagctgct
      781 ccaaagttgc cgcagctcga accaggggag actacagcgc ctgtaaggtt cgaaagacat
      841 gaaggcaagc ttgaacaaga gtcttgggct cggctcaaag ctcaagccca agagagaagg
      901 atatcgccct ctggggttgt ggctttagtt ttgtgtgaag tgttacgttg ctggagcgaa
      961 gaagattggt ttacattgaa ttttccggtc tcagaccgga tgccggtcag tgatgacatt
     1021 gatttagtgg tcggggattt tacgaataca ttgcttgtcc cttacgaaac tgaggtcgaa
     1081 gatacgtttg aagctcgtgg ccgacggtta caggatgcga tttgggaggc acttgaccat
     1141 cgacttttta ctggggtaga ggtattgcgt gagttggctc gaatccgccg ctctggtcgt
     1201 gaaccactga tgccagtagt attgacaagc cttctcggac atccaggtcg acacgatgtt
     1261 tccctgttgg gtcgggaggt attcggggtt tcacaaactc cacaggtgac gcttgatgtc
     1321 caggttcgag agtcagaggg cacactgtat ttcaaatggg actacctaac tggagtcatt
     1381 cgacctgacg tgattgaggc gatgtttggt gcgttcagta atctgctgca acagcttacg
     1441 gatgattccg aagtttggaa acgtacttgg ctagatcttc gaccagcgtc gcagatagct
     1501 gtacgaaacg aggtcaatgc aacggaggaa cctgttcccg aagttcactt acgtgatttg
     1561 ctactagagc gcctcagaga tagtgcgaac gaaccagccg tcattgatat ccatggtgaa
     1621 tacacctggg atgagattgg tcgtgcagct acgcacgtta aaatgatgat ccagcgggtt
     1681 tgtaactcaa gtgatcggtt tgtggggatc atgctaccta agggggtcgc acaatatgag
     1741 gcggtttatg gttgtctgtt ggctgggatt ggctacgttc ctatagatat cgatctacct
     1801 caagagcggg tgcgtactat actcgcacag gcaggagtac gcattgtgat tgcatcacct
     1861 gatactatgg tgcctgttgg tgtccacaag atcgaacatg tgactgctgg ccccggtagt
     1921 tggatgcaac aaaatactga ctttgttatt gaacctgtct cagaagacta ttcaccgtat
     1981 gtaattttca catcaggctc aactggcgaa ccgaagggcg ttgagattcc ggaagccgct
     2041 gtggtaaacc atattttcga tgttgttgaa cgcttcggtc tggataagtc tatccgacat
     2101 ctcgccacag ctgcactaca ctttgatatg tctgtcttcg atgttttcgg ccccttagtg
     2161 catggaggtt ccgttgtggt acccgaacct gcagctggcc ctactccaga agcttggctg
     2221 agactacatc gccaatgtag ggtgacattt tgggcatgtg tccccgctgt tatggatcta
     2281 gtatgctccg tggctgaaac agcgcttacg gtacacccga ttgagtctgt aaccaatatc
     2341 gtgatggctg gtgactggat cccactgtcc ctactaccgc gagctaggtc tgttttcccg
     2401 tctgctcgga tgtactcatg tgggggccca actgagacta caaactggtc agtaatccat
     2461 gaaatcaacg aagacgaagg tagtatctgt cgttcggtca tttatggctc accgatgcgg
     2521 aattccaaat atcacattgt tagcgagaac tggattgacc gccctgactg ggtgcctggc
     2581 gaaatgctgg tagaaagtga tatatcattg gcacgtgggt accttggaaa gcctgaactc
     2641 acgcagtgcg tattcgttta tcaccctcgg accggacgcc agatgtaccg cacaggagac
     2701 ttgggccgtt accttcccaa tggtgagata gagatcctcg gacgtgtcga caaccagatc
     2761 aagataaaag gaatcaggat cgagcttggt gaaatcgaga gagcggccga agcctgtgac
     2821 ggggtatcga gagcgtgcgc cgttgcattg cctggccaag atggaaggcc aaagcagatc
     2881 gggctagctt atatcggaga atccaatctc gatgatgcaa taatcaagga gctcacacat
     2941 cgcttaccta actacatggt gccaaagact attaaaaaag taccgaatct acccctatca
     3001 aaaaacggaa aggtggatgt aagagcattg cgtgaaagat tattaaagac cgacacaaaa
     3061 gagttaaata ctggggatcg aagaaccatt ctacgagagg tcatacgcgt gatatcggtc
     3121 caactcgccc aaccagtaat actaccagag gacaatttta ttcaattagg tggagactcg
     3181 ctgaccgcta tgaatctcaa aatcgaattg gagtccaaat tatcgataac agtttcactt
     3241 gagagcatca tgctgactga gacgattggt gagtttgcca atgagcttgc ggaaaggatg
     3301 aagctatgaa caaaaaacaa ttgtatctag aatggccgga atatgtagag gcatatctga
     3361 atggacaaac taaactacaa gggttacacg agaaggttga gttgcgcggg caagaaatat
     3421 tagagtacgt tgaggtgcaa tcaccattct accgtgaaca tctgcgtacc caagtgggtt
     3481 tcacaagtga gttcaaggtt ctggatggaa tacctttcac taccaagagt gaccttcgtt
     3541 ctgctggtac ggaggtctgt tgtgtcccga tggatgaaat cgctgcgtat tatgagacga
     3601 ctgggactac ggggaaatca acgccgtgcc cacgagcttc aattgacgtc gagactagcg
     3661 gggcatacgt tcagaatgct gttaaggaag tataccaatc aacattcggt acgatgcatg
     3721 ctctcactgc aatcatgggg ccatcagagt tatatgcctt cggggatacg tatggtgaag
     3781 tatgtcggaa tctaggtatt ccattcgtgc ggttatggcc ggagagccct cgagtcggtc
     3841 ttgaaaaagc tgctgatctc attacgggtc ttggtgtaaa gtcattaata tgctcacctg
     3901 ctgttgccct tgcccttgct cggttctata agagccttgg aatcgatccc tgcaagactc
     3961 cggtgagtca gatccttgtg ttaggagagt tatgcactcc tgagatgctt tcaaacatat
     4021 ctcggatttg gggtgcaaat tgtactcatg gtctgtatgg gtcgcaagag acacatgcag
     4081 tagctacagg ttgttcagaa ggaaatctac atctttctga aacgaactat attgttgaga
     4141 ctataccgat aaatgggctt gatggtgatg taggtgagtt gtgccttacc atgctcgtcc
     4201 ctggtgctaa gccgttaatc cgatttcgta caggggattt agcaagccta tacccgtcaa
     4261 gtgtttgtgg gtgtggaaat ccgtcccgct gtatacggat atttggtcgg gtcgacgacg
     4321 ttatgacaat cggtggctgc cagatgtttc ctgcttcaat cgaatcggct gttttaaggg
     4381 cagccgatag ggtttgggga tatcaggtcg atatcaggat ttcgaacaat ggcactgacg
     4441 aattagaagt agcgatggtt gctgaggtcg ggaatcagtc ttttgatcag attcaaaagg
     4501 agatagcgga tcattttggt gtcccggtaa ggttggtgtt gcaagattgt ttggatccgc
     4561 ggacggagac tggagcttat atttcctgga aacacgcccg aatccgagat tggaggccta
     4621 cactatgaat acgcaagaac agaaagattc atgggtcgtc gagcaaagtg tggttcctga
     4681 agccgcgccg gtagcacaag tgcggcggcg tgtattaagt ttataccctc gatcggcctt
     4741 atttgagacg gggactggtt ttcaagctgt cgatggtaaa caaacaatgt tcatggttga
     4801 tttgctgttt gagctcatac tagtagatgg cacttggcaa gtattcggca gtggtccttt
     4861 tgagatgaaa accagcgaat gtagcgaggt agttaatgat cacactaagc ccttatggga
     4921 gcgcatgaga aaggtgacac agatccttgc gttgccggag ggtttcccgc tcgtgttggt
     4981 tctggggtat agtgcagctc gttttgttga acatctaccg cggatagcta ttgaaaaggg
     5041 agaaccggag gtggtattgc gcgtctacag acacgtcgtg cgctacaacg gtgctgaagg
     5101 aggtgcccaa gtcgatgtat tgaagtgtag acacgatgag gtcacagata ccgaaatttt
     5161 aatcgcaact cttaccgatc ccgaaataga tctggctcca cgtggtccat gggatttagg
     5221 agttattcag ggtttaacgg agcggagcac cttctgcgat gcggtgaacc gtgccaaaga
     5281 acatattcgt gctggtgaca tctatcaggt tcagttgtgt cggtgcgcaa tatcgagtgc
     5341 gaggattccc ccattagacc tctatgaaag actcgcatca attaatccgg ctccgtatat
     5401 gtattacttg gaccttggcg accggcatgt ggtgtcctcc agcccagagc tcatgatccg
     5461 tagtggtgat ggtggtgctc aagttcgccc catcgcggga acaatggcgc aggaagatca
     5521 gcggggcagt cgcttggacc acattcccaa agaggcggcc gagcatctga tgcttgtgga
     5581 tctggcgcgc aatgatttag cacgttgcgc tatcccagga ggtgtggatg taacctcgtt
     5641 tatgcagatg gatgcatacg gtccactttt tcacttggtc tcgactgttg agactccggt
     5701 ccgtgcagat tccgacatat gggacttgat tgaagcaaat tttccggcgg gcacaatgac
     5761 aggtgcaccc aaagtccgtg cgatggagat tatcgccgag ctcgaagata ctgcacgagg
     5821 gttgttcact ggttgcgctg ggtatatcaa tggagagaac agtggtgtgc ttgccctgac
     5881 catcagaaca atcgtcggtg actctggtcg ctacgtattg cgagctgctg cgggtattgt
     5941 ggctgactcg aaggcatcgg ctgaatggga cgaggcggga gcgaaaatac ggtcgtttgc
     6001 cagagcgata ggagggagcg tatgagagtt ttattggtag atgcctttga tagctttgta
     6061 ttcgtcattg cacagtatta cgcaaaggtt ggcgcacaca cgaaggtggt acgtgtgaat
     6121 gatgacccaa taagtcagta ccagcggtgg aagcctcaac ttctagtcct ggggccaggt
     6181 ccaggaacac cgcaggaaca tggataccca gaaataattc gtgcagcagg tgaacatcaa
     6241 gctatattcg gcgtctgttt gggccaccaa gctattgggg agttttttgg ctgtaagctc
     6301 aaaaatgcac ctactatcga gcacggcaag aaagctcagg tgcaacacga cggacaaggt
     6361 atcttttgcc atgtcccatc gcccgtcact gtagtccggt accattcact ggctatcacc
     6421 gatcccggta cggaaaacga actggttaca tccgcatttt ctcaagaaga tggcgtggta
     6481 atgggggtgc gtcatcgtac gagaccaatc gaaggtgtgc agtttcaccc cgagagtatc
     6541 ggaacggagc acgggatcac aatgatacgg aactcgctgg cgttggctaa ctaccctagc
     6601 ttaatgtcga gctgattact tcggccagat tataccgaca ataattcttt tctagttctt
     6661 tcttgttgga aataacatag gtatagtcct agggttggct tattctaact gagtatagcg
     6721 gtggatcagg tctaaacaat gggatccacc tcagattata taatgatgca attaccaaaa
     6781 agattggagt taaaagaaat gatacagaca tcaaaaaacg aaattgaagt gataagtgag
     6841 gtgcgatgga aggcccttgc tgttatttcg atcatccagt tcatactatt gcttgacgcc
     6901 actgtggtta atgtggcatt accgcatata aagcaggatt taggattcac tgctgcgtcc
     6961 ttaacgtggg tggtgaatgc ttacctcgta gctgccggat cgctgctact gctaggtggt
     7021 cgaatcggtg atgcctttgg gttgcgacgt accttccaaa taggtatcgc agttttcggt
     7081 gtattttcac tcgtggcaac tttatcctct aacgcgagca tgcttattgt aggacgcgct
     7141 ggtcaggggg tgggtgaggc tctcactgct gcaactggtt tagcgatggt atccttattg
     7201 ttcccttcag gccctgaacg tggcaaagca ttttcgatct gggcggctct cggtggattc
     7261 gggtcaatcg tcggggttat gctatctggt gtactaactg aatatctgtc gtggcgctgg
     7321 gtctttggga tcaacatccc aatcatcttg atcctctatg tgactgttgt gctgttggtc
     7381 ccgaagttat ccaaccaggc aaccgcgcgg ttggacattg gtaatgcttt ggtattggca
     7441 ttttcggttt ctgtgatagt tttgggcatc attggttcag gcattgagca gctattatgg
     7501 ctccgcgtca cattgtctct cttgggtatg ctcggcatca tcggggtgct tcaacgttgc
     7561 aaacggtctg gtgacggcat tatacccgcc cggctaatgg ctcgttcgcc gcggatcatt
     7621 ggttatgcca ttgttgcaat cttagccggt acatctggtg ctttgtttta ccttggtgtc
     7681 ctactattgc aagattccct tggcatgacc ccaatgcaag caggtatggc ttggctgcca
     7741 ttttgcctcg gctttttccc aggcttattc ctttttcagt ttattataaa acggtggagt
     7801 tcaagggcgg ctgctcttgt tggtctcaca gtctcagcct taggtttctt actgtttgca
     7861 gttggagtaa ctacacaaag ctactgggtt gggatgatgc cagcaatgat tgtgacgtct
     7921 gtcggctttg gctgtgtttc accagtctct caatatctcg caactttcgg gctctctgag
     7981 gtggatgcag gagccggttc aggcattacg tcaacgatcc agcaactgtt taacgtaggg
     8041 ggagtgaccc ttcttgcggc cgttgctatt agtattacgg acggtactgg cgcatctaca
     8101 ataatcaatc caaaaggatt cattgtttca ttcgtgctag ccgctgtggc aataatctgc
     8161 ggggcagttc ttattttggt tagagggagt gctctagcat catctccaga caaccaggag
     8221 agcttaaccg atggaaggga atcatagggc agagggggtt tccctaggta cagccttttg
     8281 atcgaggcgt aaagtagatg catcgtcaaa gatgaccttt gggcttatat ctgtgagtca
     8341 aaggcaaagg atccaacttc taatggaaaa gttgaggaaa tgcgaggaca tctgtaacag
     8401 ccgagtctgt agtagcaaaa cgcccgatac agatagtttc agcaataaga aagctatttt
     8461 ttggtggatg aatagtctga cttgatttga ttgatagcgt tgatctgaaa ggctacgaaa
     8521 aagcgagaaa aacgaagaaa atatttaatg agagaggagg atattcagat gcagcagaaa
     8581 tacctagtcc cacctgtagg acgccagctc ataacagtcc ctagcacggc ggggctacgt
     8641 gaaaaatggg tcggagcaat gcttgacaaa acgggccgcg cctatgctgg agcattacgc
     8701 ctcgacttct caccgagttg cgaatcagat aaagctctca acgctgtagc tgatacgttg
     8761 gcccggcatc caatcctagc cgcccacttc cagattgaag gtgacatcct tgtcggctat
     8821 gcccccacag agtctagtct gcgtgaagtg ctcagggagc aaatgcgcac caagcacact
     8881 ggtattacta atgaggcgtt tcttgcgcat cgtgcaagac aatctagtga gccaggactt
     8941 aggctagcgt cagtttcgga tgataatggg ttacacgtgt ggattggatt ttggacctat
     9001 acttgcgatg gggcatcaat cgacctacta atcgaggaaa ttgctaatcg ctaccttggc
     9061 aagtccttga aatcgtcacg gagctgggag gagtatgccc agatggaggt cactcaaaca
     9121 gtaaagcaag tattagactc acggactcgg gttaatgttt accctgtgcc agggccatac
     9181 ggcattgatg cagttcgcgc cactccgcgc ggtactgtag gacatacaca aagtgtacgg
     9241 ttcgactctc ctataccacg tagcatggtt ttaacctgcg cgagagccta ccgtgttaca
     9301 ccattcgtac tattattcgc agctttccag cgtgcggtaa gtgcggtgag tggagtcaaa
     9361 accgttgtta ggagtcccat tcgcaaaccg tcggcatcct gatgatcaca ccgtggtcgg
     9421 tcccctatcg aacacagttc cgatcacaac ccaccatgag ctgggggaac cgctagtcgc
     9481 ggtgcttaat caagtgcaac gcgcggtgat tagagcagct gaacgccaga atatcgagac
     9541 gactgcactc tatcccgaag gtacctcacc tcggaacgtg gattatgacc ttcctttccc
     9601 ccagttgttc aacgcatgga actctcaacg tgatggaacc gaaatctctc tgggtaaaac
     9661 ggaatggatg tatctc
//
