LOCUS       gnlProkkaGKAFKPJL_45    4772 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GKAFKPJL_45.
ACCESSION   gnlProkkaGKAFKPJL_45
VERSION     gnlProkkaGKAFKPJL_45
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 20:33:48
            Original ID  :: gnl|Prokka|GKAFKPJL_45
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4772
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..75)
                     /locus_tag="ctg44_1"
                     /transl_table=11
                     /translation="MRNNDNIRILANPSVSHGEPLHISE"
     protocluster    1..4772
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[2913:4161]"
                     /cutoff="10000"
                     /detection_rule="(t2pks2 or ksIII)"
                     /neighbourhood="20000"
                     /product="PKS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2914..4161
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(t2pks2 or ksIII)"
                     /neighbourhood="20000"
                     /product="PKS-like"
                     /protocluster_number="1"
     protocluster    1..4772
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[1650:2913]"
                     /cutoff="20000"
                     /detection_rule="(Chal_sti_synt_C or Chal_sti_synt_N)"
                     /neighbourhood="20000"
                     /product="T3PKS"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      1651..2913
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(Chal_sti_synt_C or Chal_sti_synt_N)"
                     /neighbourhood="20000"
                     /product="T3PKS"
                     /protocluster_number="2"
     cand_cluster    1..4772
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(t2pks2 or ksIII)"
                     /detection_rules="(Chal_sti_synt_C or Chal_sti_synt_N)"
                     /kind="neighbouring"
                     /product="PKS-like"
                     /product="T3PKS"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     region          1..4772
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="PKS-like"
                     /product="T3PKS"
                     /region_number="1"
                     /rules="(t2pks2 or ksIII)"
                     /rules="(Chal_sti_synt_C or Chal_sti_synt_N)"
                     /tool="antismash"
     CDS             complement(121..870)
                     /NRPS_PKS="Domain: ECH (8-247). E-value: 3.6e-73. Score:
                     237.7. Matches aSDomain: nrpspksdomains_ctg44_2_ECH.1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023:enoyl-CoA hydratase (Score: 203.1; E-value:
                     6.9e-62)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg44_2"
                     /transl_table=11
                     /translation="MTHSVVELIEIESAIIQVKMQDRTHKNAFSQELTDGLIQAFKYIR
                     QNPKYKAVILTGYDNYFASGGTQEGLLRIQQGLTKFTDDNLYSLALDCEIPVIAAMQGH
                     GIGGGFVMGLFADLVILSRESVYTANFMKYGFTPGMGATFIVPKKLGFSLAQEILLNAG
                     SYRGADLEKRGVPFKVLPRAEVLDYAVELAQELAEKPRNSLVTLKDHLVAPLRNQLPRV
                     IEQELMMHEETFHHEEVKSRIKGLYGN"
     aSDomain        complement(130..846)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg44_2_ECH.1"
                     /evalue="3.60E-73"
                     /label="ctg44_2_ECH.1"
                     /locus_tag="ctg44_2"
                     /protein_end="247"
                     /protein_start="8"
                     /score="237.7"
                     /tool="antismash"
                     /translation="IEIESAIIQVKMQDRTHKNAFSQELTDGLIQAFKYIRQNPKYKAV
                     ILTGYDNYFASGGTQEGLLRIQQGLTKFTDDNLYSLALDCEIPVIAAMQGHGIGGGFVM
                     GLFADLVILSRESVYTANFMKYGFTPGMGATFIVPKKLGFSLAQEILLNAGSYRGADLE
                     KRGVPFKVLPRAEVLDYAVELAQELAEKPRNSLVTLKDHLVAPLRNQLPRVIEQELMMH
                     EETFHHEEVKSRIKGLY"
     CDS             complement(902..1654)
                     /NRPS_PKS="Domain: ECH (7-241). E-value: 2.7e-60. Score:
                     195.5. Matches aSDomain: nrpspksdomains_ctg44_3_ECH.1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023:enoyl-CoA hydratase (Score: 181.1; E-value:
                     3.5e-55)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg44_3"
                     /transl_table=11
                     /translation="MTYQTIKVRFQASVCYITFHRPEANNTINDTLIEECLQVLNQXXX
                     XXXXXXXXXCFGADFQEIYQEMKRGRKQASSQEPLYDLWMKLQTGPYVTISHVRGKVNA
                     GGLGFVSATDIAIADQTASFSLSELLFGLYPACVLPFLIRRIGRQKAHYMTLMTKPISV
                     QEASEWGLIDAFDAESDVLLRKHLLRLRRLNKKGIAHYKQFMSSLDHQVSRAKATALTA
                     NQNMFSDPQNQMGIIRYVETGQFPWEDQ"
     aSDomain        complement(932..1633)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg44_3_ECH.1"
                     /evalue="2.70E-60"
                     /label="ctg44_3_ECH.1"
                     /locus_tag="ctg44_3"
                     /protein_end="241"
                     /protein_start="7"
                     /score="195.5"
                     /tool="antismash"
                     /translation="VRFQASVCYITFHRPEANNTINDTLIEECLQVLNQXXXXXXXXXX
                     XXCFGADFQEIYQEMKRGRKQASSQEPLYDLWMKLQTGPYVTISHVRGKVNAGGLGFVS
                     ATDIAIADQTASFSLSELLFGLYPACVLPFLIRRIGRQKAHYMTLMTKPISVQEASEWG
                     LIDAFDAESDVLLRKHLLRLRRLNKKGIAHYKQFMSSLDHQVSRAKATALTANQNMFSD
                     PQNQMGIIRYVE"
     CDS             complement(1651..2913)
                     /gene_functions="biosynthetic (rule-based-clusters) T3PKS:
                     Chal_sti_synt_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1043:hydroxymethylglutaryl-CoA synthase (Score: 493;
                     E-value: 9.8e-150)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg44_4"
                     /sec_met_domain="Chal_sti_synt_N (E-value: 1.7e-10,
                     bitscore: 29.6, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVSAGIEAMNVFGGTAYLDVMELAKYRHLDTARFENLLMKEKAVA
                     LPYEDPVTFGVNAAKPIIDALSEAEKDRIELLITCSESGIDFGKSLSTYIHEYLGLNRN
                     CRLFEVKQACYSGTAGFQMAVNFILSQTSPGAKALVIASDISRFLIAEGGDALSEDWSY
                     AEPSAGAGAVAVLVGENPEVFQIDPGANGYYGYEVMDTCRPIPDSEAGDSDLSLMSYLD
                     CCEQTFLEYQKRVPGANYQDTFQYLAYHTPFGGMVKGAHRTMMRKVAKVKTSGIETDFL
                     TRVKPGLNYCQRVGNIMGAALFLALASTIDQGRFDTPKRIGCFSYGSGCCSEFYSGITT
                     PQGQERQRTFGIEKHLDRRYQLSMEEYELLFKGSGMVRFGTRNVKLDFEMIPGIMQSTQ
                     EKPRLFLEEISEFHRKYRWIS"
     CDS             complement(2914..4161)
                     /NRPS_PKS="Domain: PKS_KS(Enediyne-KS) (77-407). E-value:
                     2.3e-43. Score: 140.5. Matches aSDomain:
                     nrpspksdomains_ctg44_5_PKS_KS.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     PKS-like: t2pks2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 311.6; E-value:
                     1.4e-94)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg44_5"
                     /sec_met_domain="t2pks2 (E-value: 2.8e-190, bitscore:
                     621.3, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKCNLPEVVVTGVGVTASIGQGKEDFASSLLSGRHAFDVMKRSG
                     RQKDSRFIGAEIASLSYPDRLSKKMLRKASFSSRAALVTLTEAWEEAELDDADSSRIGL
                     VVGGSNFQQRENFEVYERYQDRSGFISPAYGLSFMDSDLCGICTDQFGITGLAYTVGGA
                     SASGQLAVIHAIQQVLSGEVDTCIALGALMDLSYMECEALRALGAMGTDKYADEPEKAC
                     RPFDQNRDGFIYGESCGALVIERKETALRRGLKPYAALSGWSIKLDGNRNPDPSLEGEI
                     HVIQKALERARLLPEDIDYINPHGTGSFIGDEIELKALRACRLSHAYINATKSITGHGL
                     SAAGIVEIISVLLQMKKSALHPSRNLDHPIDDSFHWVNEKSISYRIKNALSLSMGFGGM
                     NTAVCIQNIEKCGGES"
     aSDomain        complement(2941..3930)
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg44_5_PKS_KS.1"
                     /domain_subtypes="Enediyne-KS"
                     /evalue="2.30E-43"
                     /label="ctg44_5_PKS_KS.1"
                     /locus_tag="ctg44_5"
                     /protein_end="407"
                     /protein_start="77"
                     /score="140.5"
                     /tool="antismash"
                     /translation="SRAALVTLTEAWEEAELDDADSSRIGLVVGGSNFQQRENFEVYER
                     YQDRSGFISPAYGLSFMDSDLCGICTDQFGITGLAYTVGGASASGQLAVIHAIQQVLSG
                     EVDTCIALGALMDLSYMECEALRALGAMGTDKYADEPEKACRPFDQNRDGFIYGESCGA
                     LVIERKETALRRGLKPYAALSGWSIKLDGNRNPDPSLEGEIHVIQKALERARLLPEDID
                     YINPHGTGSFIGDEIELKALRACRLSHAYINATKSITGHGLSAAGIVEIISVLLQMKKS
                     ALHPSRNLDHPIDDSFHWVNEKSISYRIKNALSLSMGFGGMNTAVCIQN"
     CDS             complement(4139..4387)
                     /NRPS_PKS="Domain: PP-binding (8-74). E-value: 2.4e-11.
                     Score: 35.9. Matches aSDomain:
                     nrpspksdomains_ctg44_6_PP-binding.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg44_6"
                     /sec_met_domain="PP-binding (E-value: 3e-12, bitscore:
                     35.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKQRIFEVLITNICEVLPELDGHRFEPEDQLVELGADSVDRAEI
                     ITMVLEDLSLKIPRIELSGVKNIGELAEVLYDKVQSA"
     aSDomain        complement(4166..4363)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg44_6_PP-binding.1"
                     /evalue="2.40E-11"
                     /label="ctg44_6_PP-binding.1"
                     /locus_tag="ctg44_6"
                     /protein_end="74"
                     /protein_start="8"
                     /score="35.9"
                     /tool="antismash"
                     /translation="VLITNICEVLPELDGHRFEPEDQLVELGADSVDRAEIITMVLEDL
                     SLKIPRIELSGVKNIGELAEV"
     CDS             complement(4449..4772)
                     /locus_tag="ctg44_7"
                     /transl_table=11
                     /translation="MPAADIEKAEQNPKHKMALIFRWYFRYSSKLAISGSEHSKVDYQI
                     HCGPALGAFNQWVKGSQLENWRNRHVDEIGKKLMTETAVLLHERMQSMYQPSHETDNIK
                     IKV"
ORIGIN
        1 ttcagatata tgtaaaggct caccatgact gacagaagga ttagctaata tgcggatatt
       61 atcattattt ctcatcacgc tgcctccatt tcacctgcaa aattttatat atattttcag
      121 ttaattacca tataagcctt tgattctact tttgacttct tcatgatgaa atgtttcctc
      181 atgcatcatc agttcctgtt caatgacgcg cggcagctga ttgcgaagcg gggcaaccaa
      241 atgatctttc agtgtaacca gtgagtttct cggcttttcc gccagttctt gcgccagctc
      301 cactgcataa tctaacactt ctgcacgggg cagcacctta aagggaaccc ctcttttttc
      361 aagatcagct ccgcggtagc tgcccgcatt caataatatt tcctgggcta ggctgaaacc
      421 cagtttttta ggaacgataa aggtagcgcc cattccaggc gtaaatccgt atttcataaa
      481 attagccgta tacacgcttt ctctgctcag aatgactagg tcagcaaaga gccccatgac
      541 aaagccgcct ccaattccat ggccctgcat tgccgcaata actggaatct cacaatccag
      601 cgccagagaa taaagattgt catcagtgaa cttggtcaat ccctgttgaa ttcgtagcag
      661 tccttcttgc gttcctcctg aagcaaaata attatcatat cctgtcaaaa tgaccgcttt
      721 gtatttgggg ttctgccgta tgtatttaaa cgcttgaatt aaaccatctg taagttcttg
      781 tgaaaaagcg tttttatgag tgcgatcttg catctttacc tgaataatgg cggattcaat
      841 ttcgataagc tctacaacag aatgcgtcat gatgtcctcc taacggttct gattgaaacg
      901 gttactgatc ctcccatggg aattgtcctg tttcaacata cctgatgatt cccatttggt
      961 tttgaggatc agaaaacatg ttttgatttg cagttaaagc ggtggctttc gcacgactga
     1021 cttgatgatc aagtgagctc ataaactgtt tataatgtgc gattcctttt ttattcagcc
     1081 tccgcaaacg caataaatgc tttctcagca gcacgtcact ttcagcatca aaagcatcta
     1141 ttaaccccca ttcactggct tcctgaacgg aaatcggctt tgtcataagc gtcatataat
     1201 gcgctttctg ccggccgata cggcggatca gaaacggtaa aacacaagca gggtacaggc
     1261 cgaatagcag ctcagagaga ctgaatgacg ccgtctgatc agcaatggca atatctgtgg
     1321 cagatacaaa tccaagaccg ccggcattca cttttcccct gacatgcgaa atcgtgacat
     1381 aagggccggt ctgcaatttc atccacaaat catagagagg ctcttgggag cttgcctgtt
     1441 ttctgcccct tttcatttcc tgatagattt cttgaaaatc cgctccgaaa caannnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnncatt ggtttaacac ttgcaagcat tcttctatca
     1561 gcgtatcatt aatcgtatta ttcgcttcag gacggtgaaa ggtaatatag caaaccgacg
     1621 cttgaaagcg gacctttatc gtttgatagg tcacgagatc cacctgtact tccgatgaaa
     1681 ctcagaaatt tcttctaaaa acaagcgcgg cttctcttga gtggactgca tgatgcccgg
     1741 tatcatctca aagtccagct ttacattgcg cgtcccaaac cgcaccatcc cgctcccctt
     1801 aaacaatagc tcatactctt ccatggagag ctgataccgg cgatccaaat gcttctcaat
     1861 accaaatgtt cgctgacgct cctggccttg cggagttgta attccgctgt aaaattcaga
     1921 acagcagccg gatccgtagg aaaaacagcc aattcgttta ggcgtatcaa atctcccttg
     1981 gtctatcgtg ctggccaatg ccagaaacaa ggctgccccc ataatatttc caactcgttg
     2041 acagtagttt aaacctggtt ttacccgagt taaaaaatcc gtctcaatcc cggatgtttt
     2101 caccttcgcc accttacgca tcattgtccg gtgcgcacct ttcaccatcc cgccaaaggg
     2161 cgtatggtaa gcaagatatt gaaacgtgtc ctgataattg gcacccggta cacgcttttg
     2221 atattccaga aaggtctgtt cgcagcaatc caaatatgac attaaagaca gatcagaatc
     2281 tcccgcttca ctgtcgggaa tggggcggca cgtgtccatc acttcatagc cgtaataacc
     2341 atttgctccg ggatctattt gaaacacctc gggattttcg ccaactaaaa cggccaccgc
     2401 tcccgctccg gcgctcggtt ccgcataaga ccaatcttcg cttaaagcgt ctccgccctc
     2461 agcgattaaa aaacgtgaaa tatcactggc gataaccagt gctttggcac ccggagaagt
     2521 ttgggataat ataaaattca ccgccatctg aaatcccgct gtccctgaat aacacgcctg
     2581 tttaacctca aacaaacggc agttgcggtt gagacctaaa tattcatgga tatatgtact
     2641 taacgatttc ccaaagtcga tcccggattc agagcaagtg atcagcaatt caatccggtc
     2701 cttctctgct tcagataaag catcaataat cggcttagct gcattgactc cgaaggtcac
     2761 gggatcttca taaggcagtg ctaccgcttt ttctttcatt aataagtttt caaatctcgc
     2821 agtatctaag tgtctgtatt tggccagctc catgacatca aggtatgccg ttccgccaaa
     2881 gacattcatc gcttctattc cggctgaaac catttatgat tcgcctccac atttctcaat
     2941 gttttgtata caaaccgcgg tgttcattcc gccaaacccc atgcttaaac tcagcgcatt
     3001 tttgatgcgg tatgaaatcg atttctcatt tacccaatga aaggaatcat cgattggatg
     3061 atccaaattc ctgctcggat gcagggcaga ttttttcatt tgcagcaaaa cagaaataat
     3121 ctctacgatg cccgccgcac taagcccgtg tccggttatc gattttgttg cattgatata
     3181 agcatgggaa agccggcatg cacggagcgc ctttaattct atttcatctc caataaaaga
     3241 gcctgttcca tgaggattaa tataatcaat atcttctggt aaaagcctgg cacgctccag
     3301 tgccttctgg atgacatgaa tctctccttc taatgaagga tcaggattcc ggtttccgtc
     3361 cagtttgatt gaccagccag acaatgctgc ataaggtttg agacctcttc gtaaagccgt
     3421 ctcctttcgt tctattacca atgcaccgca cgattctcca tagataaacc cgtctctgtt
     3481 ttgatcgaag ggacggcacg ctttttcagg ttcatcagca tatttgtccg tccccatagc
     3541 gcctaaagct cttagcgcct cgcattccat ataagaaaga tccattaaag caccaagtgc
     3601 aatgcaagta tcaacttcgc ctgacaggac ttgctgaatc gcatgaatga ctgctaactg
     3661 gccgctggcc gaggctcctc ccaccgtata tgctaatccc gtgatgccaa actgatctgt
     3721 acatatcccg caaagatcgc tgtccatgaa agacaggcca tacgccggag aaataaaacc
     3781 ggagcggtct tgataccttt cataaacttc aaagttttct ctctgctgaa aatttgaccc
     3841 gccaacaacc agtccgatac gtgaggaatc tgcatcgtcc aattcagcct cctcccaagc
     3901 ctctgtgaga gttaccaatg cagcgcggct ggagaatgat gcttttcgca acattttttt
     3961 agagagtcgg tcaggataag acaatgacgc aatttcggct ccgataaacc ggctgtcttt
     4021 ttgtctgcct gaacgcttca tgacatcaaa ggcatgtctg ccggacagca gcgaggaagc
     4081 aaagtcctct ttcccttgac cgatagaggc cgtaacgcct acaccggtca ccaccacctc
     4141 aggcagattg cactttgtca taaagcacct cagctaattc accgatgttt ttcaccccgg
     4201 atagctcaat gcgagggatt tttagcgata aatcctctag caccatcgtg ataatttcag
     4261 ctctgtctac agagtcagct cctagctcaa ctaactgatc ttcaggctca aatctgtgtc
     4321 cgtctaattc aggaagcacc tcgcaaatat tggtgattag tacttcaaag attctctgtt
     4381 tatccataat gcgctctctc cttcaaggga ttattttaat gatttttata ggtatatttt
     4441 cataatattc aaacttttat ttttatgttg tctgtttcat gcgacggctg atacatcgat
     4501 tgcatccgtt catgaagcaa tactgccgtt tctgtcatta attttttccc gatttcatcc
     4561 acatggcggt ttcgccaatt ttctaattgc gatcctttga cccattgatt aaaggctccc
     4621 agcgcgggac cgcagtgaat ttgataatcg accttcgaat gttcgcttcc gctgattgcc
     4681 aattttgagc tgtatctgaa gtaccatcgg aaaatcaaag ccattttatg tttcgggttt
     4741 tgttcagcct tctcaatgtc tgccgcggga ta
//
