LOCUS       gnlProkkaCLFLPBHK_80   14185 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CLFLPBHK_80.
ACCESSION   gnlProkkaCLFLPBHK_80
VERSION     gnlProkkaCLFLPBHK_80
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 19:38:00
            Original ID  :: gnl|Prokka|CLFLPBHK_80
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14185
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14185
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2573:4185]"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2574..4185
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="1"
     cand_cluster    1..14185
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /kind="single"
                     /product="sactipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..14185
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="sactipeptide"
                     /region_number="1"
                     /rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /tool="antismash"
     CDS             27..200
                     /locus_tag="ctg79_1"
                     /transl_table=11
                     /translation="MGALFLHLKPDEYLKKLYKTKKLVEKTSFSLQAKDKARRLCPFDR
                     DYIFSGADTPIS"
     CDS             complement(164..1834)
                     /locus_tag="ctg79_2"
                     /transl_table=11
                     /translation="MNIAEQMKDVLKEEIKAAVLKAGLAEESQIPNVVLETPKDKTHGD
                     YSTNMAMQLARVAKKAPRQIAEEIVAHFDKGKASIEKLDIAGPGFINFYMNNQYLTKLI
                     PSVLEAGEAYGETNIGNGERVQVEFVSANPTGDLHLGHARGAAVGDSLCNVLSKAGYDV
                     SREYYINDAGNQINNLALSVEVRYFEALGLEKPMPEDGYRGEDIIAIGKRLAEEYGDRF
                     VNEEESERLAFFREYGLKYELEKLRKDLENFRVPFDVWYSETSLYQNGKIDKALEALRE
                     KGHVYEEDGATWFRSTTFGDDKDRVLIKKDGTYTYLLPDIAYHKDKLDRGFDKLINVWG
                     ADHHGYIPRMKAAIEALGYEKGTLEVEIIQLVHLYKNGEKMKMSKRTGKAVTMRDLIEE
                     VGLDAVRYFFAMRSADTHMDFDLDLAVSTSNENPVYYAQYAHARICSMLRQGEEQGLKP
                     AADLDFSHIQSEKEYDLLKTIGGFPEAVAEAAEKRIPHRVTNYIYDLASALHSFYNAEK
                     VIDPENEEKSRARLALMKATQITLNNALQLIGVSAPEKM"
     CDS             complement(1831..2259)
                     /locus_tag="ctg79_3"
                     /transl_table=11
                     /translation="MKQETPITLHVKSVIEDDGNQEVIEFRTTGFYYVKQNKVYLSYYE
                     EHDLGKVKTIVKVSEGEVLVMRSGAVKMNQRFVKGASTIAKYKMSFGELELKTSTKSIQ
                     SDLDEEKGRISIAYDMHVGDEQEHLHNMTITYEGGTHA"
     CDS             2574..2705
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: Subtilosin_A"
                     /gene_functions="biosynthetic-additional (sactipeptides)
                     predicted sactipeptide"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg79_4"
                     /sec_met_domain="Subtilosin_A (E-value: 9.8e-31, bitscore:
                     97.4, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKAVIVENKGCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
     CDS_motif       2574..2603
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKKAVIVENK"
                     /locus_tag="ctg79_4_sactipeptide"
                     /aSTool="sactipeptides"
                     /tool="antismash"
     CDS_motif       2604..2705
                     /prepeptide="core"
                     /locus_tag="ctg79_4_sactipeptide"
                     /peptide="sactipeptide"
                     /predicted_class
                     /score="33.00"
                     /molecular_weight="0.0"
                     /monoisotopic_mass="0.0"
                     /core_sequence="GCATCSIGAACLVDGPIPDFEIAGATGLFGLWG"
                     /aSTool="sactipeptides"
                     /tool="antismash"
                     /leader_sequence="MKKAVIVENK"
                     /leader_location="[2573:2603](+)"
     CDS             2839..4185
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: subtilosin"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Subtilosin_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SPASM"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg79_5"
                     /sec_met_domain="subtilosin (E-value: 0.0, bitscore:
                     1081.4, seeds: 6, tool: rule-based-clusters)"
                     /sec_met_domain="Subtilosin_rSAM_RRE (E-value: 5.5e-31,
                     bitscore: 98.4, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 3.6e-09, bitscore: 28.4,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.2e-23, bitscore: 75.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 5.6e-12, bitscore:
                     37.5, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 1.8e-11, bitscore: 36.0,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFIEQMFPFINESVRVHQLPEGGVLEIDYLRDNVSISDFEYLDLN
                     KTAYELCMRMDGQKTAEQILAEQCAVYDESPEDHKDWYYDMLNMLQNKQVIQLGNRAIR
                     HTITTSGSNEFPMPLHATFELTHRCNLKCAHCYLESSPEALGTVSIEQFKKTADMLFDN
                     GVLTCEITGGEIFVHPNANEILDYVCKKFKKVAVLTNGTLMRKESLELLKTYKQKIIVG
                     ISLDSVNSEVHDSFRGRKGSFAQTCKTIKLLSDHGIFVRVAMSVFEKNMWEIHDMAQKV
                     RDLGAKAFSYNWVDDFGRGRDIIHPTKDAEQHRKFMEYEQNVIDEFKDLIPIIPYERKR
                     AANCGAGWKSIVISPFGEVRPCALFPKEFSLGNIFHDSYESIFNSPLVHKLWQAQAPRF
                     SEHCMKDKCPFSGYCGGCYLKGLNSNKYHRKNICSWAKNEQLEDVVQLI"
     CDS             4198..4359
                     /locus_tag="ctg79_6"
                     /transl_table=11
                     /translation="MSPAQRRILLYILSFIFVIGAVVYFVKSDYLFTLIFIAIAILFGM
                     RARKADSR"
     CDS             4356..5075
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 125.6; E-value:
                     4.3e-38)"
                     /gene_kind="transport"
                     /locus_tag="ctg79_7"
                     /transl_table=11
                     /translation="MSILDIHDVSVWYERDNVILEQVDLHLEKGAVYGLLGVNGAGKTT
                     LINTLTGVNRNFSGRFTLCGIEAEAGMPQKTSDQQKTHRYFAADYPLLFTEITAKDYVS
                     FVHSLYQKDFSEQQFASLAEAFHFSKYINRRISELSLGNRQKVVLMTGLLLRAPLFILD
                     EPLVGLDVESIEVFYQKTREYCEAGGTILFSSHLLDVVQRFCDYAAILHNKQIQKVIPI
                     GEETDLRREFFEVIGHE"
     CDS             5068..6378
                     /locus_tag="ctg79_8"
                     /transl_table=11
                     /translation="MNNIIPIMSLLFKQLYSRQGKKDAIRIAAGLVILAVFEIGLIRQA
                     GIDESVLRKTYIILALLLMNAYMVFLSVTSQWKESYMKLSCLLPISSRSFWLAQSVVLF
                     VDTCLRRTLFFFILPLFLFGNGTLSGAQTLFWLGRFSFFTVYSIIFGVVLSNHFVKKKN
                     LMFLLHAAIFACVCLSAALMPAATLPLCAVHMLWAVVIDFPVFLQAPPQQSRMHSFMRR
                     SEFSFYKREWNRFISSKAMLLNYAVMAAFSGFFSFQMMNTGIFNQQVIYIVISALLLIC
                     SPIALLYSIEKNDRMLLITLPIKGKTMFWAKYRFYSGLLAGEFLLVVMIVGFISGRPIS
                     VLTFLQCIELLLAGAFIRLTADEKRPFFSWQTEQQLWSGFSKYRSYLFCLPLFLATLAG
                     TAVSLAVIPIAGLVIVYYLQKQDGGFFDTSKRERLGS"
     CDS             6344..7528
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg79_9"
                     /sec_met_domain="Peptidase_M16_C (E-value: 5.8e-07,
                     bitscore: 21.2, seeds: 139, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIQAKENGLEVNLLKTHQFSTISIAASFLKPIESAAEPEEETIYF
                     YGAAAYLKEQIIDDFGCAAGSRFMYSANLFFDQQLKTCGTRLIHPLYNGNLHVDALMKT
                     FADLSFPSSLSFEAVEKARNELLLKIEKKFTDPFSYSAARLAEEVFGNPMYGTGMFGRK
                     DRIKAIHPKRFHDATDFIVDLVSQQKQLNILGQVQACDVRGHAPQTSAVTSERIPVNRH
                     VFETETRSAAGPSVLTLGFDCGEMKDASDYIKIQLIDGLLGKYGHSALFKHFREKDLAV
                     YHVITRYDVMNNLLLVSICTDQLHEIDIPPRVLEAVSAFHTDERELEQAKQFLRNELLL
                     QFDSPEGLLAYMGILRRFSCTKEALLDGISAVTCRDVLQFIATINYIGAHVVRG"
     CDS             7533..8813
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_M16"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg79_10"
                     /sec_met_domain="Peptidase_M16 (E-value: 2.7e-15, bitscore:
                     48.2, seeds: 24, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKKAFFQQLDERTDIRYTDSGLKIFRLKFPRAHLRLCNVKIDFG
                     SRDVCIRAESGDTLLPYGTAHFLEHLLFWHNGRNLYSDFFAHGALLNAFTTYTDTNFMF
                     TSLPDRLRQTIPILLDALWNHSFDKKIVAQEKAVITSEIQTAHLNHQLSYHYQLISMLS
                     PSSPAAVFPAGRIEDIEALDISDLQKAYKAAYQTHRMTLFLIGGSENTETLLPPHLQLE
                     KRPDYHAERKIIPACPPALSQKMMLGDEERMEDTWTGLQIGALPGQNDLLTIKLYWDIA
                     ARILFQLDSPFFQEIQQTYRLEIDRLSAETYIYEDGGFLILHSQGTHSSAYIDVASYYV
                     TQKKEQAAAWLQYGKDSLTDAIIYDSDYVRKCFEWAAECDRCDCSFLDMYHIIQDMDAQ
                     VFLSLIDAMASSNKAIIHVSQKEAIRQ"
     CDS             8810..9511
                     /locus_tag="ctg79_11"
                     /transl_table=11
                     /translation="MKKNTVFTLLLLLIGMAAYSFGWVQAVAEAAAHYIQMINNDAVRL
                     GLLACTAALLMLPAFLYLHYVTQSVKNMTAAFQKLTQSHQSCCDFQQHNLCSRYAEDVK
                     SLRDGYKNVRQTYVMAAVLCQVIIFGCMFEIVKAVPFRLHTPPALSMGLAMLLILYLLF
                     CMRTYLRQLFRHGSLFRKVFAGVLAAAGIWWMLSFTISELLFLIILAAIQQIRSFIYKR
                     FSYRSTASLDL"
     CDS             complement(9517..10893)
                     /locus_tag="ctg79_12"
                     /transl_table=11
                     /translation="MKKNDLSALQENCFCFCDEEISREAPFQVPIDIPKGFKVDSAEAS
                     AAVTWSTDNLSCVSEPCLIQTGPETEDIGVRYAVRVQGTIMLLVSVSPVRNQYGQGDGA
                     VSVIHTEEIDQVVYYAAQSDRCPDFSQMTVEDLLIVPPFYGSPLTVAGTLVLPPSPDPQ
                     SYVFTANTGDSTVSVIDADLNTVVKTILFSAVPTNLGVTFDKAFTYVLHGNTNLVSVID
                     NKTLTIINTITVGGGPRKIEFDPTDEYAYVMAAGSIYMIDTANQSVIDVIAIPGALDFA
                     LDPNGQYVYTANGSSWSVDKYDVNTGQLVESIIDSFEFPSLIATPYAGNFAYVLNGELW
                     PKGVTEISLSPLSRGGDFNRLFETLRTIVFSLDSTRAYFLEPYSEPFLINNLYVVNTAR
                     QRIIANVPLPGAFDPAVTPDKQYIYAAQPNDNAVTVYRTSDYTAVKVIPVGAGPSAIVM
                     "
     CDS             complement(10932..11417)
                     /locus_tag="ctg79_13"
                     /transl_table=11
                     /translation="MFAYVLNFGLVQKVDLSTGEVTGTIERELIVSSIETDSPERYAYV
                     LEQEFLFNYLTVIDLNTFTISNTQELEPDGQYRMFTSGTGVYLYDDFTGNLYSVSPNGA
                     GVIGNLPQSATDYAFTPNGDFLYATRFIEQSIIVYNTDDYSEETVISLGVSPGAITI"
     CDS             complement(11450..12283)
                     /locus_tag="ctg79_14"
                     /transl_table=11
                     /translation="MRKNKLSFKEKAGAEQEECLCFCEGEASREAMFQAPIELPEGFVV
                     DPAEAVANVTWNADSLSCVSDRCLIQTGPEPDEVGVQFAVRLQGTVTLLVSVSPVRNQY
                     GQGDGAVSVIHNAEIDQVVYYSDEPDDCPDISNITIENLVVVPPFYGSPLSVTGTIVLV
                     PEPEPVYAFTANPNDQSVSVIDTNTHTVVTTIALPYNPAGIEITPDKSAVFVLHPNNNV
                     ISVIDYDTLTVTATILLDQPPRLIRFIPNHEFAYVFTGTAIYVIGIDTLTVERSI"
     CDS             12513..13658
                     /locus_tag="ctg79_15"
                     /transl_table=11
                     /translation="MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEE
                     DQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPRLSDLLLEIDKKQARLTGLLEYYFN
                     FFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFSMDY
                     ARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAVSHFQKAYSMAEAEKQPQLMG
                     RTLYNIGLCKNSQSQYEDAITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKA
                     HEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAIQGFFDFLESKMLYADLEDFT
                     IDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV"
     CDS             13642..13761
                     /locus_tag="ctg79_16"
                     /transl_table=11
                     /translation="MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI"
     CDS             13859..14170
                     /locus_tag="ctg79_17"
                     /transl_table=11
                     /translation="MSLKSVRTHFTQWNRENDVTEFETSSATVEQAAETIGVSLSRIAK
                     SLSFRGEGDQVILIVAAGDAKIDNKKSRQTFGFKARMLSPNEVLEQTGHEIGGVCPIG"
ORIGIN
        1 atacctagcc aagcagggct aggcatgtgg gagcgctttt tctgcatctg aaacctgatg
       61 aatatctaaa gaaactgtat aaaacaaaaa agcttgtaga aaaaacgtcg ttttctctac
      121 aagctaagga caaagcccga agactttgtc cttttgatcg tgattacatt ttttccggag
      181 ccgatacgcc gatcagctga agcgcattgt tcagcgtaat ttgcgttgcc ttcattaaag
      241 ccaggcgcgc acggcttttc tcttcatttt cagggtcgat gactttctct gcgttgtaga
      301 agctgtgcag agcagaggcc agatcataaa tatagtttgt gacacggtgc ggaattcgtt
      361 tttccgctgc ttccgccact gcctctggga agccgccgat cgttttcagc agatcgtatt
      421 ctttttctga ttgaatatgg ctgaaatcaa gatccgccgc cggtttaagt ccctgctctt
      481 ccccttggcg aagcatgctg cagattcggg catgtgcata ttgtgcataa tacacagggt
      541 tttcgttaga tgttgataca gcaagatcta agtcaaaatc catatgggtg tccgcacttc
      601 gcatcgcaaa gaagtaacga acggcatcca agccgacttc ttcaatcagg tcacgcatcg
      661 ttacggcttt accggtccgt ttgctcattt tcattttctc gccgtttttg taaaggtgaa
      721 cgagctgaat gatttctact tcaagcgttc ctttttcata gcccagcgct tcgattgccg
      781 ctttcatacg cgggatgtag ccgtggtgat cggcgcccca aacgttgatc agcttatcaa
      841 agccgcggtc aagcttgtcc ttatgatacg cgatatcagg aagcaggtac gtgtacgtgc
      901 cgtccttctt gatcaatacg cggtctttat catcgccaaa cgtcgtggaa cggaaccaag
      961 tcgcgccgtc ttcttcatag acatggcctt tttcgcggag cgcttcaagc gccttgtcga
     1021 tttttccatt ttggtacagt gaagtttctg aataccacac atcaaacggc acacggaagt
     1081 tttccaagtc cttgcgaagc ttttccagct catatttcag gccgtattca cggaaaaacg
     1141 cgaggcgttc actttcttct tcgttaacga aacgatcacc gtattcctca gcgagacgct
     1201 ttccgatcgc aatgatatcc tcgccgcggt agccatcctc cggcatcggt ttttccaatc
     1261 cgagtgcttc aaagtaacgg acttcaacag aaagcgccag attgttgatt tggttacccg
     1321 catcgttaat gtagtattcg cggcttacat cgtaacccgc ttttgaaagc acgttgcaca
     1381 aggaatcgcc gacagccgcc ccgcgcgcat gcccgaggtg aaggtctccc gtcggattcg
     1441 ctgatacgaa ttctacctga actcgttcac cgtttccgat gttcgtttcc ccgtatgctt
     1501 cccctgcttc cagtacagac ggaatcagct ttgttaaata ttgattgttc atatagaaat
     1561 tgataaaacc cgggccggcg atatccagtt tttcaatcga agcctttcct ttatcaaaat
     1621 gggcgacaat ctcttcagcg atttggcgcg gcgctttttt tgccactctt gccagctgca
     1681 tcgccatatt cgttgagtag tcgccgtgtg ttttatcttt cggtgtttct aaaacaacat
     1741 tgggaatctg gctttcttca gccagtcccg ctttcagaac ggccgctttg atttcttctt
     1801 tcagcacgtc cttcatttgt tccgcaatgt tcatgcgtga gttcctccct catacgtgat
     1861 cgtcatatta tgtaaatgct cctgctcgtc tccgacatgc atgtcatatg cgatgctgat
     1921 acggcctttt tcttcatcga gatcagattg aatcgatttt gtgcttgttt tcagctcaag
     1981 ctcaccaaat gacattttat atttagcaat tgtggaagct ccctttacga aacgctgatt
     2041 cattttgaca gcgcctgacc tcatgaccag gacttcgcct tcgcttactt tcacaatcgt
     2101 tttgactttg cctaagtcgt gttcttcata ataagataga tacactttat tttgttttac
     2161 atagtaaaat ccggtggtgc ggaattcgat gacttcttgg tttccgtcat cctcgatcac
     2221 agacttcaca tggagtgtta tcggtgtctc ttgcttcatt ctgacacgtc cttttcatca
     2281 ttccactttg acttgaatag tataatcatt cggtttgtaa acttcaactg cttctatctt
     2341 accatcattg ctcatcagat ttgaagataa acctcataaa aagcatttcc ttatatagaa
     2401 gagaaaatca tatcactaat tacctttagt aaatgttaca tttttccgaa atctatcatt
     2461 tccttttcac atttttttca aaatatatgt attgaattag taatttgata gttttaagat
     2521 aaaagtacaa catagatctg ttagaaaaac aaaaaaaaag ggaggattca attatgaaaa
     2581 aagctgtcat tgtagaaaac aaaggttgtg caacatgctc gatcggagcc gcttgtctag
     2641 tggacggtcc tatccctgat tttgaaattg ccggtgcaac aggtctattc ggtctatggg
     2701 ggtaaggatc tcccaaaagg gcatagtcat tctactatgc cctttttaag taaattacaa
     2761 tttttaacta aaatctacct cttggatata catacacaac cgtttttcat ttgaatcata
     2821 tgtatagggg atggtatttt gtttatagag cagatgtttc catttattaa tgaaagtgta
     2881 agagttcacc agcttcctga gggcggcgtg ttagaaatcg actacttgcg cgataatgtc
     2941 tccatttctg actttgagta tttggatctc aacaaaacag cttacgagct ctgcatgcgc
     3001 atggatggcc aaaaaacagc tgagcagatt ttagctgagc aatgtgcagt gtatgatgaa
     3061 tcaccggaag accataaaga ttggtattac gacatgctca acatgctcca gaacaagcag
     3121 gttatccagc ttggaaaccg ggccatccgc cataccatca ccacgagcgg aagcaatgaa
     3181 tttccgatgc ccctgcacgc cacctttgaa ctgacgcacc gctgtaattt gaaatgcgcc
     3241 cactgttatt tggaaagctc gcctgaagcg ctcggcaccg tgtcgattga gcaattcaaa
     3301 aaaacggctg atatgctgtt tgataacggt gtattgacat gcgaaatcac aggcggagaa
     3361 atttttgtcc atccaaacgc caatgagatt cttgactatg tgtgtaaaaa gttcaaaaaa
     3421 gtcgctgtct taacaaacgg aacactcatg cgaaaagaga gcctggagct tttgaagact
     3481 tacaagcaaa aaatcatcgt cggcatttct cttgatagtg tcaattccga ggtccatgac
     3541 tcctttagag ggagaaaagg ctcttttgcc caaacttgta aaacgataaa attgttgagt
     3601 gaccacggta tatttgtcag agtcgccatg tctgtcttcg aaaaaaacat gtgggaaatc
     3661 cacgatatgg cccaaaaggt tcgggatctc ggagcgaagg cgttttctta caattgggtt
     3721 gacgatttcg gaagaggcag ggatattatc catccaacga aagacgccga gcagcaccgc
     3781 aagtttatgg aatacgagca aaatgtgatt gatgagttta aagatctgat tccgattatt
     3841 ccctatgaga gaaaacgcgc ggcaaattgc ggcgctggct ggaagtccat tgtgatcagc
     3901 ccgttcggcg aagtacgtcc ttgcgccctc tttccaaagg aattttcatt gggaaatatt
     3961 tttcatgatt cctatgaaag catctttaac tcccctctcg tccataaact gtggcaagcg
     4021 caggcgccgc ggttcagcga acattgcatg aaagacaaat gcccgttcag cggctattgc
     4081 ggaggctgtt acttaaaagg gctgaactct aacaaatatc accgaaaaaa catttgctct
     4141 tgggcgaaaa atgaacaatt agaagacgtg gtccagctta tttaggaggg aaacgatttg
     4201 tcaccagcac aaagaagaat tttactgtat atcctttcat ttatctttgt catcggcgca
     4261 gtcgtctatt ttgtcaaaag cgattatctg tttacgctga ttttcattgc cattgccatt
     4321 ctgttcggaa tgcgcgcgcg gaaggctgac tcgcgatgag cattttggat atacacgatg
     4381 tatccgtttg gtatgaacgg gacaacgtca tcttagaaca agtggattta catttagaaa
     4441 aaggcgccgt ttacgggctg cttggggtaa acggcgccgg aaaaacgaca ctgattaata
     4501 cgctgacagg cgtgaaccgc aatttcagcg ggcgctttac actgtgcgga attgaagcgg
     4561 aggccggcat gccgcagaaa acatcagatc agcagaagac tcaccgttac ttcgccgccg
     4621 attatccgct gctatttaca gaaattacgg cgaaggacta tgtgtctttc gtccattcgc
     4681 tttatcaaaa ggatttttca gagcagcagt ttgccagttt ggccgaggcc tttcattttt
     4741 cgaaatacat caacaggaga atttcggagc tgtccttggg gaacaggcaa aaggttgtat
     4801 tgatgacagg cttattgctg cgcgctcccc tatttatttt ggatgagccg ctcgtcggtt
     4861 tggatgtgga atcgatagag gttttttatc agaaaacgcg cgagtactgt gaagcaggcg
     4921 ggaccatttt gttttcttct catctgctcg atgtcgtgca aagattttgt gattacgcgg
     4981 ccattcttca caacaaacag atccaaaagg tcattccgat tggagaggag accgatctgc
     5041 ggcgggaatt tttcgaggtg atcggccatg aataacataa tccccatcat gtctttgctg
     5101 ttcaaacagc tttacagccg gcaagggaaa aaggacgcca tccgcattgc cgcaggcctt
     5161 gtcattctgg ccgtatttga aatcgggctg atccgccagg ccggcattga tgaatcggtg
     5221 ttgcgcaaaa cgtatatcat actcgcgctt ctcttaatga acgcatatat ggtgtttctt
     5281 tctgtgacat ctcaatggaa ggaatcttat atgaaactga gctgcctgct gccgatttct
     5341 tcacggagct tttggctcgc ccagagtgtc gttttgtttg tcgatacctg tttgagaaga
     5401 acgttattct tttttatttt accgctgttc ttatttggaa acggaacgct gtcaggggcg
     5461 caaacattgt tttggctcgg caggttttcg ttttttaccg tttactccat tattttcgga
     5521 gttgtgctaa gcaaccactt cgtcaaaaag aagaacttga tgtttctgct gcatgcggcg
     5581 atattcgcct gtgtatgtct cagcgccgct ttgatgccgg ccgccacgct tccgctttgc
     5641 gcggtgcata tgctgtgggc ggtggtcatt gactttcctg tctttctgca ggcgcctccg
     5701 cagcagagca ggatgcattc atttatgcgg cgatctgagt tttcgtttta caaaagagaa
     5761 tggaaccgat ttatctcttc taaagcgatg ctgttaaatt acgcggtaat ggcggcgttc
     5821 agcggcttct tttcgttcca gatgatgaat accggcatct tcaatcagca agtgatttat
     5881 atcgtgattt ccgcgcttct gctcatctgc tcgccgatcg cccttttgta ctcgattgaa
     5941 aaaaatgacc ggatgctgct catcacgctt ccgatcaagg ggaaaacgat gttttgggcg
     6001 aaatatcgct tttattcagg cctattggca ggcgaatttc tccttgtcgt gatgattgtg
     6061 ggtttcatca gcggccgtcc gatttcagtt ctcacttttt tgcagtgcat tgagttgctg
     6121 ctggcgggag cttttatcag gttaactgcg gatgaaaaaa ggcccttctt cagctggcag
     6181 acggagcagc agctatggag cggtttctca aaatatcggt cttatctgtt ttgtcttccc
     6241 ctattcttag ccacattagc cggcaccgct gtatccttgg cggtcattcc gatagccggt
     6301 ctggtcatag tctattacct gcaaaaacag gatggtggtt tctttgatac aagcaaaaga
     6361 gaacggcttg gaagttaacc tgctgaagac tcatcaattt tcaactatat caatcgccgc
     6421 gagctttctg aaaccgatcg aaagcgcggc ggaacctgag gaagaaacga tttattttta
     6481 cggcgccgcc gcatacctga aggagcaaat cattgacgat ttcggttgtg ctgccggcag
     6541 ccgtttcatg tactcagcga atctcttttt tgatcagcag ctgaaaacct gcgggacccg
     6601 tttgattcac ccattatata atgggaatct tcatgttgac gccctgatga aaacttttgc
     6661 agacttgtct ttcccttctt ctctttcatt cgaagcagtt gaaaaggcca ggaatgagct
     6721 gctgctgaaa atcgaaaaga aattcactga tcccttctcc tactcggcgg cgcgcttggc
     6781 tgaggaggtc ttcggaaacc cgatgtacgg cacaggcatg tttggccgga aagacaggat
     6841 aaaggcgatt catcctaaac ggtttcacga cgcaacggac tttatcgttg atttagtgtc
     6901 ccagcaaaaa cagctgaaca tattgggaca agtacaagca tgtgatgttc ggggacatgc
     6961 cccgcaaacg tccgctgtaa cgtccgaaag aatacccgta aaccggcatg tttttgaaac
     7021 agagacacgc agcgctgcag gtccttccgt cttgacactc ggatttgact gcggagagat
     7081 gaaagatgcc agcgattata tcaaaataca gctaatagac ggattactgg gaaagtacgg
     7141 ccattccgct ttattcaagc acttcagaga gaaagatctg gcagtctatc acgttatcac
     7201 ccgttacgat gtcatgaaca atttgctgct tgtttcaata tgtacagatc agcttcatga
     7261 gatagacatt cccccgcgcg ttttggaagc tgtctcagct tttcatacag atgagcggga
     7321 actagaacag gcaaagcagt ttttacgcaa tgaattgctg ctgcaattcg attctcccga
     7381 aggtctattg gcctatatgg ggatcctgcg ccgcttctcc tgcacgaaag aagccttgct
     7441 ggatggcatc tcagcggtta catgccgtga tgtattgcaa ttcatagcga ccatcaatta
     7501 tatcggagct catgtagtaa ggggatgaaa agatggaaaa gaaagccttt tttcaacagc
     7561 tggatgaacg aaccgatatc agatacacag acagcggact aaaaatattc agattgaaat
     7621 tcccccgcgc ccacctgcgg ctgtgcaatg taaaaatcga ttttggaagc cgggatgtat
     7681 gtatacgggc agaatccggt gatacactgc ttccgtatgg cacagcgcat tttttagagc
     7741 atcttctctt ctggcataac ggccgcaatc tgtacagtga tttttttgcg catggcgcgc
     7801 ttttaaacgc attcactacc tacaccgata ccaatttcat gtttacctca ttaccggatc
     7861 ggctcagaca aacgatcccc atcctgcttg atgctttatg gaaccactca tttgacaaga
     7921 agattgtcgc tcaggaaaaa gctgtgatta caagcgaaat tcaaacagca caccttaacc
     7981 atcagctctc atatcattat cagctgatca gcatgctttc tccctcctca ccagctgccg
     8041 ttttccccgc aggcagaatc gaagatattg aggcgcttga catcagtgat ttacaaaaag
     8101 cctataaggc tgcctaccaa acccatcgga tgacgctgtt tttgattggg ggctcagaaa
     8161 atacagaaac gctattgcct cctcacctcc agctggaaaa gcgtccggat tatcatgctg
     8221 aaagaaaaat catcccggca tgccctccag ccctctcaca aaaaatgatg ctgggtgatg
     8281 aggagcggat ggaagataca tggaccggat tacagatcgg cgcacttccc gggcaaaacg
     8341 atctgttgac gatcaaactt tattgggata tcgcggctcg tattctattt caattagatt
     8401 caccgttttt tcaagagatt cagcaaacgt accgcctgga aatcgatcgt ttgtcagccg
     8461 agacttatat ctatgaggat ggcggtttct tgatccttca cagtcaaggg acccattctt
     8521 cagcttatat cgatgtggct tcatactatg tcacacagaa gaaagaacag gctgccgcct
     8581 ggcttcaata cggcaaggac tctttgacag acgcgatcat ttatgacagc gattatgtgc
     8641 gtaaatgctt cgagtgggca gccgagtgtg acaggtgcga ttgctcgttt cttgacatgt
     8701 accacatcat tcaagatatg gatgcgcagg ttttcttgtc tctcattgat gcaatggcat
     8761 cttctaacaa agcgatcatt cacgtttctc agaaagaggc gatcagacaa tgaaaaaaaa
     8821 cactgtcttt acccttttat tgctgcttat aggtatggcg gcttattcct tcggctgggt
     8881 tcaggccgtt gccgaagccg ccgcccacta tatacagatg atcaacaatg atgctgtacg
     8941 gctcggactg ctggcttgta ccgctgccct cctgatgctg cctgcttttc tctatcttca
     9001 ctatgtcacc caatctgtga aaaacatgac agcagctttt cagaaactca cacagtctca
     9061 tcaaagctgc tgtgactttc agcaacacaa cttgtgcagc cgatatgcag aagacgtcaa
     9121 atcgctgcgt gacggctata aaaacgtccg ccaaacatac gtgatggctg ccgtgttgtg
     9181 ccaagtcatc atattcggct gcatgttcga aatcgttaaa gccgtccctt tccgccttca
     9241 cacgccccct gccctttcaa tgggacttgc gatgctactg atcctgtatt tattgttttg
     9301 catgcgaacg tatttgcggc agcttttccg gcacggcagc ctgttcagaa aagtgtttgc
     9361 gggagtgctg gcagcagccg ggatttggtg gatgctcagt ttcactataa gtgaactgct
     9421 gtttctgatt atcctggctg ccatccagca aatcagatca tttatttata agcgattctc
     9481 ttatcgcagc accgcttctc ttgatctata aaatagctac atcacaatcg cagacgggcc
     9541 cgcgccaacc ggaatcacct tcacggctgt ataatcactt gtgcggtaca cagtcacagc
     9601 attgtcgttg ggctgtgccg cataaatata ctgtttgtcc ggcgttacgg cggggtcaaa
     9661 agcgccgggg agaggcacgt ttgcaatgat cctctgtctg gccgtattaa ctacgtataa
     9721 gttattaatg agaaacggtt cactatatgg ctctagaaaa taagctctag tactatcgag
     9781 agaaaataca atcgtcctca gcgtttcaaa taaacggtta aagtcccctc ccctgcttaa
     9841 tggtgacaaa ctaatctccg ttacaccttt cggccacagc tcaccattca acacgtacgc
     9901 aaaatttcca gcgtaaggag ttgcaattaa actgggaaat tcaaatgaat ctatgatact
     9961 ttctaccaac tgcccagtat ttacatcata tttatcaacc gaccaactgc ttccattagc
    10021 ggtatagaca tactgtccat tcggatcaag agcaaagtct aaagcgccgg gtatcgcgat
    10081 tacatcaatt actgattgat tggccgtgtc aatcatatat atagatccgg cggccatcac
    10141 gtaggcgtac tcatctgttg gatcaaattc gatctttctc ggtcctcctc caacagtaat
    10201 ggtatttatg atggttaatg ttttattatc aatcactgag acaaggttgg tattcccatg
    10261 gaggacatac gtgaatgctt tatcgaaagt aacgcctaga ttagttggca cagcagaaaa
    10321 caggattgtt tttacaacag tgtttaaatc tgcatcaatg accgaaaccg tgctgtctcc
    10381 agtattggca gtaaacacat acgattgagg gtctggcgat ggcggcaaaa caagtgtccc
    10441 ggccaccgtg agcggactgc cgtagaaagg cggcacgatg agaagatctt ccactgtcat
    10501 ctgactaaaa tcaggacagc ggtcagactg tgctgcataa taaacaactt gatcaatctc
    10561 ttcagtatga atcacagaaa ccgccccgtc tccttgtcca tattgatttc gcacaggcga
    10621 cacactgaca agaagcataa tggtcccttg tactctcaca gcatatcgaa caccaatatc
    10681 ttctgtttcc ggcccagtct ggattaagca aggctcactg acacaggaca aattgtctgt
    10741 actccatgta acagccgctg acgcttcggc tgaatcaacc ttgaagccct tagggatatc
    10801 tattggcact tgaaacggcg cttcccggct aatttcctca tcacaaaaac agaaacaatt
    10861 ttcctgtaaa gcagaaagat cattcttttt catgaattca cgtcctttta aaaatgcaca
    10921 acatgcagcg gttaaatcgt aatggcaccc ggtgaaaccc caagagatat cacagtttcc
    10981 tcagaataat catctgtgtt gtaaacaatg atgctctgct ctataaaacg agttgcatac
    11041 agaaaatcgc cattcggggt aaacgcatag tctgttgctg attgcggaag atttccaatt
    11101 acacctgctc cattcggact gacagaatat aaattgccgg taaagtcgtc atataaatac
    11161 acacctgttc cgctcgtaaa cattctatat tggccgtcag gctccagttc ttgggtattg
    11221 ctgatcgtaa acgtatttaa gtcaataacc gtcaaataat tgaataagaa ttcttgttct
    11281 aagacatacg cataccgctc cggagaatct gtttctatgg atgatacgat aagctctcgt
    11341 tcaatcgttc ctgtgacttc accggtggat aagtccactt tttgcactaa cccaaaatta
    11401 agaacatagg cgaacaaccc attggggtca attgcaatat caaaaccttc tatatagacc
    11461 tctcaacagt caacgtatca attccaatca cataaatggc agtaccggtg aaaacataag
    11521 caaactcatg attagggata aatctaatca atcgaggcgg ctgatccagc aatatagttg
    11581 ctgtcactgt taatgtgtca taatcgatga cagaaatcac attattgttg ggatgtaaga
    11641 caaacacagc gcttttatct ggcgtaattt caatacctgc cggattgtac ggaagagcaa
    11701 tcgttgtcac aacagtatgg gtgtttgtat caatcacaga aactgattga tcatttggat
    11761 tcgctgtaaa cgcgtagacg ggttccggct ccggtacaag cacaatcgtc cccgtcaccg
    11821 ataaaggact gccgtaaaac ggcggaacaa caacaaggtt ctcaatcgtg atgttactga
    11881 tatccgggca atcatccggt tcatcggaat aatagacgac ttgatcgatt tctgcattgt
    11941 gaatgacgga aacagcccca tccccttgtc catattgatt tcgcacaggt gagacactga
    12001 cgagcagcgt aacggttccc tgcagacgca ctgcaaattg aacaccgacc tcatcaggct
    12061 ctggccccgt ttgaattaag caacggtcac tgacacaaga aaggctgtct gcattccacg
    12121 tcacgtttgc aacagcttca gccggatcaa cgacaaatcc ttcaggtaat tcaatcggcg
    12181 cttgaaacat ggcttctcgg cttgcttctc cctcacaaaa acataagcac tcttcttgtt
    12241 ctgctcctgc tttttcttta aaggaaagct tgttttttct cattccttca cttccttttc
    12301 agacatacac tgctcacgca gatataacag aatatggaag agtaacgtgt ttgcttgtac
    12361 aaattactga gggtaaaagc ttctttttga aaatatatct ccaaacatgc ggtgtaacga
    12421 aagaaaaaat tgcggttttt ttgcgcttct cacaagaatg ttgatgataa aatatgacat
    12481 atatagaatt atgaagggag ggatttgcaa acgtgacagg tgtcatatct tcttcttcca
    12541 tcggagaaaa gattaacgaa tggtatatgt acatacgccg attcagcata cccgatgcag
    12601 aatatttgcg acgagaaatc aagcaagagc tggatcaaat ggaagaagat caagaccttc
    12661 atttgtacta ttcactgatg gagtttcggc acaacctgat gcttgagtac cttgaaccgt
    12721 tagaaaaaat gaggattgag gaacagccga gactgtctga tctgctgctt gagattgata
    12781 aaaaacaggc tcgtttaaca ggtctgcttg agtactattt taatttcttc agaggcatgt
    12841 atgagctgga tcagcgggaa tatctgtcgg ctattaaatt tttcaaaaag gccgaaagca
    12901 agctgatatt cgttaaggat cggatagaga aagctgagtt tttctttaag atgtctgaat
    12961 cttattacta tatgaaacaa acgtattttt caatggacta tgcacggcaa gcatatgaaa
    13021 tatacaaaga aaatgaagct tataatataa gattgctgca gtgtcattct ttatttgcca
    13081 ccaatttttt agatttaaaa cagtatgagg atgccgtctc acattttcaa aaagcttatt
    13141 ctatggcaga agctgaaaag cagccccaat taatgggaag aactttgtac aatatcgggc
    13201 tttgtaaaaa cagccaaagc caatatgagg atgccataac atatttcaaa agagcaatag
    13261 ctgtttttga agaatcaaat attcttcctt ccttacctca agcgtatttt ttaattacac
    13321 agatccatta taaattagga aaaatggata aagctcatga atatcatagt aagggaatgg
    13381 cttattcaca aaaggccgga gatgtaatat atttatcaga gtttgaattt ttgaaatctt
    13441 tatacttatc aggcccggat gaagaagcaa ttcaaggatt ttttgatttt ctcgaaagta
    13501 aaatgttgta tgctgatctt gaagatttca ctattgatgt ggcaaaatat tatcatgaac
    13561 gtaaaaattt tcaaaaagct tctgcttatt ttttgaaggt ggaacaagta aggcaactta
    13621 ttcaaggagg agtgagtttg tatgaaattg aagtctaaac tattactctc ttgtctggct
    13681 ctaagcactg tgttcgtggc aacaactatt gcaaatgcac ctacacacca aattgaagtt
    13741 gcacaacgag gaatgattta accgccgtcc atcggcggtt tttttgtccc ctctttaccc
    13801 agtctcccaa tccattctat gattttttca ataatcttaa aaatagtgga aatgcagcat
    13861 gtctctaaaa agtgtgagaa cccactttac tcaatggaat cgagaaaatg atgtgacgga
    13921 gttcgaaacg tcgagtgcga cagttgaaca ggcagctgag acaatcggcg taagcctgtc
    13981 tagaatcgcc aagtccctgt ccttcagagg ggaaggagat caggtgattc tgattgtggc
    14041 agccggcgat gccaagatcg acaacaaaaa gtccaggcaa acatttggct ttaaagcaag
    14101 aatgctctct cctaatgagg tactggagca gacaggccat gaaattggag gagtttgccc
    14161 aattggatag actcatgatc ctgag
//
