GCA_036208645.1 - 13 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaPIBGAFCF_2 (original name was: gnl|Prokka|PIBGAFCF_2)

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	terpene	562,798	583,541
Region 2.2	PKS-like	668,253	709,497	butirosin A/butirosin B	Saccharide	7%

gnlProkkaPIBGAFCF_5 (original name was: gnl|Prokka|PIBGAFCF_5)

Region	Type	From	To	Most similar known cluster		Similarity
Region 5.1	NRPS,transAT-PKS,betalactone	32,343	142,750	fengycin	NRP	93%

gnlProkkaPIBGAFCF_6 (original name was: gnl|Prokka|PIBGAFCF_6)

Region	Type	From	To	Most similar known cluster		Similarity
Region 6.1	NRPS	10,275	38,068	surfactin	NRP:Lipopeptide	47%

gnlProkkaPIBGAFCF_8 (original name was: gnl|Prokka|PIBGAFCF_8)

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	NRPS	1	15,263	surfactin	NRP:Lipopeptide	39%

gnlProkkaPIBGAFCF_11 (original name was: gnl|Prokka|PIBGAFCF_11)

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	transAT-PKS,NRPS,T3PKS	21,388	131,516	bacillaene	Polyketide+NRP	100%

gnlProkkaPIBGAFCF_12 (original name was: gnl|Prokka|PIBGAFCF_12)

Region	Type	From	To	Most similar known cluster		Similarity
Region 12.1	NRPS-like	1	1,186	thermoactinoamide A	NRP	100%

gnlProkkaPIBGAFCF_14 (original name was: gnl|Prokka|PIBGAFCF_14)

Region	Type	From	To	Most similar known cluster		Similarity
Region 14.1	T3PKS	305,081	346,181
Region 14.2	terpene	396,738	418,621

gnlProkkaPIBGAFCF_19 (original name was: gnl|Prokka|PIBGAFCF_19)

Region	Type	From	To	Most similar known cluster		Similarity
Region 19.1	other	1	25,166	bacilysin	Other	100%

gnlProkkaPIBGAFCF_26 (original name was: gnl|Prokka|PIBGAFCF_26)

Region	Type	From	To	Most similar known cluster		Similarity
Region 26.1	RiPP-like,NRP-metallophore,NRPS	14,273	66,048	bacillibactin	NRP	100%

gnlProkkaPIBGAFCF_27 (original name was: gnl|Prokka|PIBGAFCF_27)

Region	Type	From	To	Most similar known cluster		Similarity
Region 27.1	lanthipeptide-class-iii	48,228	64,646

gnlProkkaPIBGAFCF_35 (original name was: gnl|Prokka|PIBGAFCF_35)

Region	Type	From	To	Most similar known cluster		Similarity
Region 35.1	NRPS	1	9,790	surfactin	NRP:Lipopeptide	8%

Region	Type	From	To	Most similar known cluster	Similarity
Region 2.1	terpene	562,798	583,541
Region 2.2	PKS-like	668,253	709,497	butirosin A/butirosin B	Saccharide	7%
Region 5.1	NRPS,transAT-PKS,betalactone	32,343	142,750	fengycin	NRP	93%
Region 6.1	NRPS	10,275	38,068	surfactin	NRP:Lipopeptide	47%
Region 8.1	NRPS	1	15,263	surfactin	NRP:Lipopeptide	39%
Region 11.1	transAT-PKS,NRPS,T3PKS	21,388	131,516	bacillaene	Polyketide+NRP	100%
Region 12.1	NRPS-like	1	1,186	thermoactinoamide A	NRP	100%
Region 14.1	T3PKS	305,081	346,181
Region 14.2	terpene	396,738	418,621
Region 19.1	other	1	25,166	bacilysin	Other	100%
Region 26.1	RiPP-like,NRP-metallophore,NRPS	14,273	66,048	bacillibactin	NRP	100%
Region 27.1	lanthipeptide-class-iii	48,228	64,646
Region 35.1	NRPS	1	9,790	surfactin	NRP:Lipopeptide	8%

Compact view

gnlProkkaPIBGAFCF_2 - Region 1 - terpene

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Location: 562,798 - 583,541 nt. (total: 20,744 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaPIBGAFCF_2 - Region 2 - PKS-like

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Location: 668,253 - 709,497 nt. (total: 41,245 nt) Show pHMM detection rules used

PKS-like: (t2pks2 or ksIII)

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NRPS/PKS domains

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

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Similar known gene clusters from MIBiG 3.1

gnlProkkaPIBGAFCF_5 - Region 1 - NRPS,betalactone,transAT-PKS

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Location: 32,343 - 142,750 nt. (total: 110,408 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
betalactone: (HMGL-like and AMP-binding)

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core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 32342 - 142750:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Glu - D-Tyr) + (Ile) + (mal - Asn) + (D-Tyr - D-Asn - Gln - Pro) + (D-Asn - Ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 32342 - 109587:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - Asn) + (D-Tyr - D-Asn - Gln - Pro) + (D-Asn - Ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 91260 - 142750:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Glu - D-Tyr) + (Ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg5_65

Search NORINE for peptide: strict or relaxed

AMP-binding (268..663): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1778..2185): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg5_66

Search NORINE for peptide: strict or relaxed

AMP-binding (268..662): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DALSVGEVVK
Nearest Stachelhaus code(s):: Tyr DALSVGEVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1778..2173): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3287..3692): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: DAQDLGVVDK
Nearest Stachelhaus code(s):: Gln DAQDLGVVDK (100% 8Å match); Ile DAQDLGVVDK (74% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4334..4730): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code(s):: Pro DVQFIAHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg5_67

Search NORINE for peptide: strict or relaxed

CAL_domain (36..478): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 614.1
NH2: 18.8
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (2956..3351): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg5_69

PKS_AT (6..286): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 75.6
inactive: 33.5
Methoxymalonyl-CoA: 20.3
Propionyl-CoA: 13.0
Methylmalonyl-CoA: 11.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg5_87

AMP-binding (12..445): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: -GLHIAAPVK
Nearest Stachelhaus code(s):: Z-ahmohOrn FGLHIASTCK (56% 8Å match); Ile DGLFIGIPVK (32% 8Å match)
Stachelhaus code match:: 60% (weak)

ctg5_91

Search NORINE for peptide: strict or relaxed

AMP-binding (473..866): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFYGITFK
Nearest Stachelhaus code(s):: Ile DAFFYGITFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg5_92

Search NORINE for peptide: strict or relaxed

AMP-binding (10..239): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWHFGGVDK
Nearest Stachelhaus code(s):: Glu DAWHFGGVDK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (870..1262): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DGTITAEVAK
Nearest Stachelhaus code(s):: Tyr DGTITAEVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaPIBGAFCF_6 - Region 1 - NRPS

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Location: 10,275 - 38,068 nt. (total: 27,794 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS domains

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NRPS/PKS modules

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 10274 - 38068:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Glu - Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg6_35

Search NORINE for peptide: strict or relaxed

AMP-binding (318..720): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: DAKDLGVVDK
Nearest Stachelhaus code(s):: Glu DAKDLGVVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1364..1761): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFMMGMVFK
Nearest Stachelhaus code(s):: Leu DAFMMGMVFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaPIBGAFCF_8 - Region 1 - NRPS

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Location: 1 - 15,263 nt. (total: 15,263 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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NRPS/PKS domains

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 15263:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg8_2

Search NORINE for peptide: strict or relaxed

AMP-binding (469..867): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DGFFLGVVFK
Nearest Stachelhaus code(s):: Ile DGFFLGVVFK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaPIBGAFCF_11 - Region 1 - NRPS,T3PKS,transAT-PKS

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Location: 21,388 - 131,516 nt. (total: 110,129 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
NRPS: cds(Condensation and (AMP-binding or A-OX))
T3PKS: cds(Condensation and (AMP-binding or A-OX))

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 21387 - 131516:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - ccmal - ohmal - mal) + (ccmal - mal - ohmal) + (mal) + (Me-ccmal - ohmal) + (Ala - ccmal - ccmal - Me-ccmal) + (mal - mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg11_41

Search NORINE for peptide: strict or relaxed

AMP-binding (494..898): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSNMAIIYK
Nearest Stachelhaus code(s):: Ala DVSNMAIIYK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg11_45

Search NORINE for peptide: strict or relaxed

CAL_domain (74..476): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 22.2
shikimic_acid: 0.0
NH2: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (1145..1540): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code(s):: Gly DILQLGMIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg11_50

PKS_AT (4..282): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 99.9
inactive: 30.9
Methoxymalonyl-CoA: 28.6
Methylmalonyl-CoA: 15.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg11_51

PKS_AT (7..293): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 29.5
inactive: 17.6
Methoxymalonyl-CoA: 11.9
Benzoyl-CoA: 9.4
Isobutyryl-CoA: 8.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg11_52

PKS_AT (4..285): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 98.2
inactive: 43.3
Methoxymalonyl-CoA: 36.6
Methylmalonyl-CoA: 24.6
Propionyl-CoA: 24.5
2-Methylbutyryl-CoA: 5.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

gnlProkkaPIBGAFCF_12 - Region 1 - NRPS-like

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Location: 1 - 1,186 nt. (total: 1,186 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 1186:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg12_1

Search NORINE for peptide: strict or relaxed

AMP-binding (13..227): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaPIBGAFCF_14 - Region 1 - T3PKS

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Location: 305,081 - 346,181 nt. (total: 41,101 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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No matches found.

gnlProkkaPIBGAFCF_14 - Region 2 - terpene

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Location: 396,738 - 418,621 nt. (total: 21,884 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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core biosynthetic genes

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Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaPIBGAFCF_19 - Region 1 - other

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Location: 1 - 25,166 nt. (total: 25,166 nt) Show pHMM detection rules used

Region on contig edge.

other: (LmbU or Neocarzinostat or bcpB or frbD or mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)

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core biosynthetic genes

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		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

gnlProkkaPIBGAFCF_26 - Region 1 - NRP-metallophore,NRPS,RiPP-like

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Location: 14,273 - 66,048 nt. (total: 51,776 nt) Show pHMM detection rules used

Region on contig edge.

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))
NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 14272 - 66048:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 14320 - 66048:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - Thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg26_43

Search NORINE for peptide: strict or relaxed

AMP-binding (31..435): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVVNK
Nearest Stachelhaus code(s):: diOH-Bz PLPAQGVVNK (97% 8Å match); diOH-Bz or Sal PLPAQGVVNK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg26_45

Search NORINE for peptide: strict or relaxed

AMP-binding (456..855): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1513..1929): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaPIBGAFCF_27 - Region 1 - lanthipeptide-class-iii

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Location: 48,228 - 64,646 nt. (total: 16,419 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-iii: (micKC and not PKS_KS)

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

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No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg27_62

ctg27_59

Leader:

MRDVLELQQLSEGTEVEASKGKGG

allorf_56993_57124

Leader:

MRDVLELQQLNEGTEVEASKGKGG

Shared core:

LLTLITTVVGTSTISNNCG

Core with dehydrations:

LLDhbLIDhbDhbVVGDhbDhaDhbIDhaNNCysG

MIBiG 3.1 matches for ctg27_59

34.8% BGC0002111 (multiple genes): Lanthipeptide: andalusicin A or andalusicin B

9

VGTSTISNNC

18

V STISNNC

14

VWASTISNNC

23

30.4% BGC0002111 (EVG22_32245): Lanthipeptide: andalusicin A or andalusicin B

11

TSTISNNC

18

STISNNC

16

ASTISNNC

23

antiSMASH-DB 4.0 matches for ctg27_59

100.0% NC_014551.1and 21 others:

NC_014551.1 Lanthipeptides: BAMF_RS41665_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20950_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20940_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22090_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20775_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20765_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21840_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21835_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04865_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04860_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03890_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03880_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19010_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19005_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20385_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18880_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18875_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00405_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19500_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19860_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19855_lanthipeptide

Lanthipeptides: BAMF_RS41675_lanthipeptide

1

LLTLITTVVGTSTISNNCG

19

LLTLITTVVGTSTISNNCG

1

LLTLITTVVGTSTISNNCG

19

94.7% NC_014551.1and 5 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20945_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20770_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03885_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20380_lanthipeptideNZ_JALAUK010000033.1 Lanthipeptides: MOE38_RS18580_lanthipeptide

Lanthipeptides: BAMF_RS41670_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

LLTLITTVVGTSTISNNC

1

LLTLITTVVGTSTISNNC

18

94.7% NZ_CP026010.1and 9 others:

NZ_CP054584.1 Lanthipeptides: HT135_RS21845_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04870_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19000_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21930_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21925_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21920_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18885_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19510_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19505_lanthipeptide

Lanthipeptides: BS11774_RS22085_lanthipeptide

1

LLTLITTVVGTSTISNNCG

19

LLTLITT+VGTSTISNNCG

1

LLTLITTIVGTSTISNNCG

19

with 22 more sequence matches

89.5% NZ_CP026010.1and 8 others:

NZ_CP054584.1 Lanthipeptides: HT135_RS21825_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04850_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19020_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21910_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19790_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18865_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00410_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19490_lanthipeptide

Lanthipeptides: BS11774_RS22075_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

LLTLITT+VGTSTISNNC

1

LLTLITTIVGTSTISNNC

18

84.2% NC_014551.1and 5 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20930_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20755_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03870_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20395_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19840_lanthipeptide

Lanthipeptides: BAMF_RS41655_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

LLTLITT+VG+STISNNC

1

LLTLITTIVGSSTISNNC

18

56.5% NZ_LN868937.1: Lanthipeptides: BN2144_RS20095_lanthipeptide

1

LLTLITTVVGTSTISNNCG

19

T+ TT++GTSTISNNCG

4

WYTIFTTLIGTSTISNNCG

22

54.5% NC_014551.1and 13 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20935_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22080_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20760_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21830_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04855_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03875_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19015_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20390_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21915_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19785_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18870_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19495_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19845_lanthipeptide

Lanthipeptides: BAMF_RS41660_lanthipeptide

2

LTLITTVVGTSTISNNC

18

TL+TT++GTSTISN+C

4

WTLLTTLIGTSTISNSC

20

54.5% NZ_LN868937.1: Lanthipeptides: BN2144_RS20100_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

T+ TT++GTSTISNNC

4

WYTIFTTLIGTSTISNNC

21

45.5% NZ_LN868937.1: Lanthipeptides: BN2144_RS19915_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

T +T +V TSTISN+C

5

WFTSVTLLVTTSTISNHC

22

45.0% NZ_JAFFJY010000011.1: Lanthipeptides: JVX89_RS12320_lanthipeptide

7

TVVGTSTISNNC

18

T VG STISN C

9

TTVGLSTISNGC

20

36.8% NC_016109.1and 5 others:

NC_016109.1 Lanthipeptides: KSE_RS43700_lanthipeptideNZ_CP068437.1 Lanthipeptides: JK628_RS03400_lanthipeptideNZ_CP068437.1 Lanthipeptides: JK628_RS03395_lanthipeptideNZ_CP068437.1 Lanthipeptides: allorf_0778008_0778106_lanthipeptideNZ_CP068437.1 Lanthipeptides: JK628_RS03405_lanthipeptide

Lanthipeptides: KSE_RS43695_lanthipeptide

12

STISNNCG

19

STISN+CG

2

STISNHCG

9

36.8% NC_016109.1: Lanthipeptides: KSE_RS43705_lanthipeptide

12

STISNNCG

19

STISN CG

2

STISNYCG

9

36.8% NZ_BMVC01000018.1and 1 other:

NZ_BMVC01000018.1 Lanthipeptides: IE282_RS34445_lanthipeptide

Lanthipeptides: IE282_RS34450_lanthipeptide

12

STISNNCG

19

ST+SNNCG

3

STVSNNCG

10

36.8% NZ_CP014222.1: Lanthipeptides: VN23_RS10390_lanthipeptide

7

TVVGTSTISNNCG

19

V ST+SN+CG

2

QVADWSTVSNHCG

14

36.4% NZ_CP066711.1: Lanthipeptides: JGZ56_RS07825_lanthipeptide

2

LTLITTVVGTSTISNNC

18

L+ + + +G STISN+C

6

LSTLLSFLGGSTISNHC

22

34.8% NZ_CP035727.2and 5 others:

NZ_CP035727.2 Lanthipeptides: EVG22_RS16230_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16225_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16220_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16215_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16210_lanthipeptide

Lanthipeptides: EVG22_RS16235_lanthipeptide

9

VGTSTISNNC

18

V STISNNC

14

VWASTISNNC

23

34.6% NZ_CP003923.1and 4 others:

NZ_CP042163.1 Lanthipeptides: FPV17_RS21685_lanthipeptideNZ_CP117834.1 Lanthipeptides: PQ477_RS10640_lanthipeptideNZ_KV917380.1 Lanthipeptides: BK583_RS20905_lanthipeptideNZ_SNUX01000002.1 Lanthipeptides: E2L03_RS20340_lanthipeptide

Lanthipeptides: BLEG1_RS21170_lanthipeptide

2

LTLITTVVGTSTISNNC

18

+T TV+G ST SN+C

10

ITTTRTVIGWSTASNSC

26

33.3% NZ_SMBT01000005.1: Lanthipeptides: EV682_RS11315_lanthipeptide

7

TVVGTSTISNNCG

19

T ST+SN+CG

1

TPADWSTVSNHCG

13

31.6% NZ_BMVC01000018.1and 4 others:

NZ_FOHE01000012.1 Lanthipeptides: BMX40_RS20340_lanthipeptideNZ_JALMDK010000006.1 Lanthipeptides: MZC64_RS26320_lanthipeptideNZ_JANBBG010000004.1 Lanthipeptides: allorf_1567890_1567982_lanthipeptideNZ_JANBBG010000004.1 Lanthipeptides: allorf_1567737_1567829_lanthipeptide

Lanthipeptides: IE282_RS34455_lanthipeptide

12

STISNNC

18

ST+SNNC

3

STVSNNC

9

31.6% NZ_CP113837.1and 1 other:

NZ_CP113837.1 Lanthipeptides: OU787_RS28895_lanthipeptide

Lanthipeptides: OU787_RS28905_lanthipeptide

12

STISNNCG

19

ST+SN+CG

2

STVSNHCG

9

31.6% NZ_CP113837.1: Lanthipeptides: OU787_RS28900_lanthipeptide

12

STISNNCG

19

ST+SN CG

2

STVSNYCG

9

30.4% NZ_CP014806.1and 3 others:

NZ_CP014806.1 Lanthipeptides: ATY39_RS17975_lanthipeptideNZ_CP014806.1 Lanthipeptides: ATY39_RS17970_lanthipeptideNZ_CP014806.1 Lanthipeptides: ATY39_RS17965_lanthipeptide

Lanthipeptides: ATY39_RS17980_lanthipeptide

9

VGTSTISNNC

18

V STISN+C

14

VFASTISNHC

23

30.4% NZ_CP035727.2: Lanthipeptides: EVG22_RS16240_lanthipeptide

11

TSTISNNC

18

STISNNC

16

ASTISNNC

23

30.0% NZ_CP066711.1: Lanthipeptides: allorf_1652598_1652744_lanthipeptide

7

TVVGTSTISNNC

18

TV G STISN C

14

TVYGLSTISNKC

25

13.2% NZ_JAIQCM010000003.1: Lanthipeptides: K9V56_RS08295_lanthipeptide

11

TSTISNNCG

19

TSTIS CG

33

TSTISGKCG

41

ctg27_60

Leader:

MKDVLELQQLNEGTEVEASKGKGG

Core:

LLTLITTIVGSSTISNNC

Core with dehydrations:

LLDhbLIDhbDhbIVGDhaDhaDhbIDhaNNCys

MIBiG 3.1 matches for ctg27_60

34.8% BGC0002111 (multiple genes): Lanthipeptide: andalusicin A or andalusicin B

9

VGSSTISNNC

18

V +STISNNC

14

VWASTISNNC

23

30.4% BGC0002111 (EVG22_32245): Lanthipeptide: andalusicin A or andalusicin B

11

SSTISNNC

18

+STISNNC

16

ASTISNNC

23

antiSMASH-DB 4.0 matches for ctg27_60

100.0% NC_014551.1and 5 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20930_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20755_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03870_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20395_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19840_lanthipeptide

Lanthipeptides: BAMF_RS41655_lanthipeptide

1

LLTLITTIVGSSTISNNC

18

LLTLITTIVGSSTISNNC

1

LLTLITTIVGSSTISNNC

18

94.4% NZ_CP026010.1and 18 others:

NZ_CP026010.1 Lanthipeptides: BS11774_RS22085_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21825_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21845_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04850_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04870_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19020_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19000_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21910_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21930_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21925_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21920_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19790_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18865_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18885_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00410_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19490_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19510_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19505_lanthipeptide

Lanthipeptides: BS11774_RS22075_lanthipeptide

1

LLTLITTIVGSSTISNNC

18

LLTLITTIVG+STISNNC

1

LLTLITTIVGTSTISNNC

18

88.9% NC_014551.1and 27 others:

NC_014551.1 Lanthipeptides: BAMF_RS41675_lanthipeptideNC_014551.1 Lanthipeptides: BAMF_RS41665_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20945_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20950_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20940_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22090_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20770_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20775_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20765_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21840_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21835_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04865_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04860_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03885_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03890_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03880_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19010_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19005_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20380_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20385_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18880_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18875_lanthipeptideNZ_JALAUK010000033.1 Lanthipeptides: MOE38_RS18580_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00405_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19500_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19860_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19855_lanthipeptide

Lanthipeptides: BAMF_RS41670_lanthipeptide

1

LLTLITTIVGSSTISNNC

18

LLTLITT+VG+STISNNC

1

LLTLITTVVGTSTISNNC

18

with 12 more sequence matches

50.0% NC_014551.1and 13 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20935_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22080_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20760_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21830_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04855_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03875_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19015_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20390_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21915_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19785_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18870_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19495_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19845_lanthipeptide

Lanthipeptides: BAMF_RS41660_lanthipeptide

2

LTLITTIVGSSTISNNC

18

TL+TT++G+STISN+C

4

WTLLTTLIGTSTISNSC

20

50.0% NZ_LN868937.1and 1 other:

NZ_LN868937.1 Lanthipeptides: BN2144_RS20095_lanthipeptide

Lanthipeptides: BN2144_RS20100_lanthipeptide

1

LLTLITTIVGSSTISNNC

18

T+ TT++G+STISNNC

4

WYTIFTTLIGTSTISNNC

21

45.0% NZ_JAFFJY010000011.1: Lanthipeptides: JVX89_RS12320_lanthipeptide

7

TIVGSSTISNNC

18

T VG STISN C

9

TTVGLSTISNGC

20

40.9% NZ_LN868937.1: Lanthipeptides: BN2144_RS19915_lanthipeptide

1

LLTLITTIVGSSTISNNC

18

T +T +V +STISN+C

5

WFTSVTLLVTTSTISNHC

22

36.4% NZ_CP066711.1: Lanthipeptides: JGZ56_RS07825_lanthipeptide

2

LTLITTIVGSSTISNNC

18

L+ + + +G STISN+C

6

LSTLLSFLGGSTISNHC

22

34.8% NZ_CP035727.2and 5 others:

NZ_CP035727.2 Lanthipeptides: EVG22_RS16230_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16225_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16220_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16215_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16210_lanthipeptide

Lanthipeptides: EVG22_RS16235_lanthipeptide

9

VGSSTISNNC

18

V +STISNNC

14

VWASTISNNC

23

33.3% NZ_BMVC01000018.1and 6 others:

NZ_BMVC01000018.1 Lanthipeptides: IE282_RS34445_lanthipeptideNZ_BMVC01000018.1 Lanthipeptides: IE282_RS34455_lanthipeptideNZ_FOHE01000012.1 Lanthipeptides: BMX40_RS20340_lanthipeptideNZ_JALMDK010000006.1 Lanthipeptides: MZC64_RS26320_lanthipeptideNZ_JANBBG010000004.1 Lanthipeptides: allorf_1567890_1567982_lanthipeptideNZ_JANBBG010000004.1 Lanthipeptides: allorf_1567737_1567829_lanthipeptide

Lanthipeptides: IE282_RS34450_lanthipeptide

12

STISNNC

18

ST+SNNC

3

STVSNNC

9

30.8% NZ_CP003923.1and 4 others:

NZ_CP042163.1 Lanthipeptides: FPV17_RS21685_lanthipeptideNZ_CP117834.1 Lanthipeptides: PQ477_RS10640_lanthipeptideNZ_KV917380.1 Lanthipeptides: BK583_RS20905_lanthipeptideNZ_SNUX01000002.1 Lanthipeptides: E2L03_RS20340_lanthipeptide

Lanthipeptides: BLEG1_RS21170_lanthipeptide

2

LTLITTIVGSSTISNNC

18

+T T++G ST SN+C

10

ITTTRTVIGWSTASNSC

26

30.4% NZ_CP014806.1and 3 others:

NZ_CP014806.1 Lanthipeptides: ATY39_RS17975_lanthipeptideNZ_CP014806.1 Lanthipeptides: ATY39_RS17970_lanthipeptideNZ_CP014806.1 Lanthipeptides: ATY39_RS17965_lanthipeptide

Lanthipeptides: ATY39_RS17980_lanthipeptide

9

VGSSTISNNC

18

V +STISN+C

14

VFASTISNHC

23

30.4% NZ_CP035727.2: Lanthipeptides: EVG22_RS16240_lanthipeptide

11

SSTISNNC

18

+STISNNC

16

ASTISNNC

23

26.7% NZ_CM000752.1and 15 others:

NZ_CM000752.1 Sactipeptides: BTHUR0007_RS37430_sactipeptideNZ_JABGBR020000007.1 Sactipeptides: HOV72_RS15955_sactipeptideNZ_JABGBR020000007.1 Sactipeptides: HOV72_RS15950_sactipeptideNZ_JYPH01000039.1 Sactipeptides: TU52_RS33370_sactipeptideNZ_JYPH01000039.1 Sactipeptides: TU52_RS33365_sactipeptideNZ_NJPZ01000025.1 Sactipeptides: CE087_RS29070_sactipeptideNZ_NJPZ01000025.1 Sactipeptides: CE087_RS29065_sactipeptideNZ_NJPZ01000025.1 Sactipeptides: CE087_RS29060_sactipeptideNZ_NUBO01000020.1 Sactipeptides: CN575_RS31360_sactipeptideNZ_NUBO01000020.1 Sactipeptides: CN575_RS31355_sactipeptideNZ_NUBO01000020.1 Sactipeptides: CN575_RS31350_sactipeptideNZ_NUUQ01000016.1 Sactipeptides: COI65_RS30820_sactipeptideNZ_NUUQ01000016.1 Sactipeptides: COI65_RS30815_sactipeptideNZ_NUUQ01000016.1 Sactipeptides: COI65_RS30810_sactipeptideNZ_NUXK01000018.1 Sactipeptides: COJ27_RS31850_sactipeptide

Sactipeptides: BTHUR0007_RS37435_sactipeptide

1

LLTLITTIVGSSTIS

15

LL L+T G+ST S

16

LLNLVTAATGASTAS

30

26.7% NZ_CP066711.1: Lanthipeptides: allorf_1652598_1652744_lanthipeptide

7

TIVGSSTISNNC

18

T+ G STISN C

14

TVYGLSTISNKC

25

allorf_57273_57401

Leader:

MRDVLELQQLNEGTEVEASKGKGG

Core:

LLTLITTVVGTSTISNNC

Core with dehydrations:

LLDhbLIDhbDhbVVGDhbDhaDhbIDhaNNCys

MIBiG 3.1 matches for allorf_57273_57401

34.8% BGC0002111 (multiple genes): Lanthipeptide: andalusicin A or andalusicin B

9

VGTSTISNNC

18

V STISNNC

14

VWASTISNNC

23

30.4% BGC0002111 (EVG22_32245): Lanthipeptide: andalusicin A or andalusicin B

11

TSTISNNC

18

STISNNC

16

ASTISNNC

23

antiSMASH-DB 4.0 matches for allorf_57273_57401

100.0% NC_014551.1and 27 others:

NC_014551.1 Lanthipeptides: BAMF_RS41675_lanthipeptideNC_014551.1 Lanthipeptides: BAMF_RS41665_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20945_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20950_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20940_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22090_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20770_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20775_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20765_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21840_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21835_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04865_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04860_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03885_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03890_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03880_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19010_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19005_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20380_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20385_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18880_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18875_lanthipeptideNZ_JALAUK010000033.1 Lanthipeptides: MOE38_RS18580_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00405_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19500_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19860_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19855_lanthipeptide

Lanthipeptides: BAMF_RS41670_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

LLTLITTVVGTSTISNNC

1

LLTLITTVVGTSTISNNC

18

89.5% NZ_CP026010.1and 18 others:

NZ_CP026010.1 Lanthipeptides: BS11774_RS22075_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21845_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21825_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04870_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04850_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19000_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19020_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21930_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21925_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21920_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21910_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19790_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18885_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18865_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00410_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19510_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19505_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19490_lanthipeptide

Lanthipeptides: BS11774_RS22085_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

LLTLITT+VGTSTISNNC

1

LLTLITTIVGTSTISNNC

18

88.9% NC_014551.1and 5 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20930_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20755_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03870_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20395_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19840_lanthipeptide

Lanthipeptides: BAMF_RS41655_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

LLTLITT+VG+STISNNC

1

LLTLITTIVGSSTISNNC

18

with 13 more sequence matches

54.5% NC_014551.1and 13 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20935_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22080_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20760_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21830_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04855_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03875_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19015_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20390_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21915_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19785_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18870_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19495_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19845_lanthipeptide

Lanthipeptides: BAMF_RS41660_lanthipeptide

2

LTLITTVVGTSTISNNC

18

TL+TT++GTSTISN+C

4

WTLLTTLIGTSTISNSC

20

52.2% NZ_LN868937.1and 1 other:

NZ_LN868937.1 Lanthipeptides: BN2144_RS20100_lanthipeptide

Lanthipeptides: BN2144_RS20095_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

T+ TT++GTSTISNNC

4

WYTIFTTLIGTSTISNNC

21

45.5% NZ_LN868937.1: Lanthipeptides: BN2144_RS19915_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

T +T +V TSTISN+C

5

WFTSVTLLVTTSTISNHC

22

45.0% NZ_JAFFJY010000011.1: Lanthipeptides: JVX89_RS12320_lanthipeptide

7

TVVGTSTISNNC

18

T VG STISN C

9

TTVGLSTISNGC

20

36.4% NZ_CP066711.1: Lanthipeptides: JGZ56_RS07825_lanthipeptide

2

LTLITTVVGTSTISNNC

18

L+ + + +G STISN+C

6

LSTLLSFLGGSTISNHC

22

34.8% NZ_CP035727.2and 5 others:

NZ_CP035727.2 Lanthipeptides: EVG22_RS16230_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16225_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16220_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16215_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16210_lanthipeptide

Lanthipeptides: EVG22_RS16235_lanthipeptide

9

VGTSTISNNC

18

V STISNNC

14

VWASTISNNC

23

34.6% NZ_CP003923.1and 4 others:

NZ_CP042163.1 Lanthipeptides: FPV17_RS21685_lanthipeptideNZ_CP117834.1 Lanthipeptides: PQ477_RS10640_lanthipeptideNZ_KV917380.1 Lanthipeptides: BK583_RS20905_lanthipeptideNZ_SNUX01000002.1 Lanthipeptides: E2L03_RS20340_lanthipeptide

Lanthipeptides: BLEG1_RS21170_lanthipeptide

2

LTLITTVVGTSTISNNC

18

+T TV+G ST SN+C

10

ITTTRTVIGWSTASNSC

26

33.3% NZ_BMVC01000018.1and 6 others:

NZ_BMVC01000018.1 Lanthipeptides: IE282_RS34445_lanthipeptideNZ_BMVC01000018.1 Lanthipeptides: IE282_RS34455_lanthipeptideNZ_FOHE01000012.1 Lanthipeptides: BMX40_RS20340_lanthipeptideNZ_JALMDK010000006.1 Lanthipeptides: MZC64_RS26320_lanthipeptideNZ_JANBBG010000004.1 Lanthipeptides: allorf_1567890_1567982_lanthipeptideNZ_JANBBG010000004.1 Lanthipeptides: allorf_1567737_1567829_lanthipeptide

Lanthipeptides: IE282_RS34450_lanthipeptide

12

STISNNC

18

ST+SNNC

3

STVSNNC

9

33.3% NZ_CP068437.1and 1 other:

NZ_CP068437.1 Lanthipeptides: JK628_RS03395_lanthipeptide

Lanthipeptides: JK628_RS03400_lanthipeptide

12

STISNNC

18

STISN+C

5

STISNHC

11

31.6% NZ_CP014222.1: Lanthipeptides: VN23_RS10390_lanthipeptide

7

TVVGTSTISNNC

18

V ST+SN+C

2

QVADWSTVSNHC

13

30.4% NZ_CP014806.1and 3 others:

NZ_CP014806.1 Lanthipeptides: ATY39_RS17975_lanthipeptideNZ_CP014806.1 Lanthipeptides: ATY39_RS17970_lanthipeptideNZ_CP014806.1 Lanthipeptides: ATY39_RS17965_lanthipeptide

Lanthipeptides: ATY39_RS17980_lanthipeptide

9

VGTSTISNNC

18

V STISN+C

14

VFASTISNHC

23

30.4% NZ_CP035727.2: Lanthipeptides: EVG22_RS16240_lanthipeptide

11

TSTISNNC

18

STISNNC

16

ASTISNNC

23

30.0% NZ_CP066711.1: Lanthipeptides: allorf_1652598_1652744_lanthipeptide

7

TVVGTSTISNNC

18

TV G STISN C

14

TVYGLSTISNKC

25

allorf_57691_57822

Leader:

MDEQMNEVLELQQLSKGSEVE

Core:

TAGWTLLTTLIGTSTISNSCNK

Core with dehydrations:

DhbAGWDhbLLDhbDhbLIGDhbDhaDhbIDhaNDhaCysNK

MIBiG 3.1 matches for allorf_57691_57822

31.8% BGC0000563 (AEA03263.1): Lanthipeptide: venezuelin

7

LTTLIGTSTISNSC

20

L TL+ T I SC

8

LLTLLNTVCIGISC

21

26.1% BGC0002111 (EVG22_32245): Lanthipeptide: andalusicin A or andalusicin B

13

TSTISNSC

20

STISN+C

16

ASTISNNC

23

26.1% BGC0002111 (multiple genes): Lanthipeptide: andalusicin A or andalusicin B

14

STISNSC

20

STISN+C

17

STISNNC

23

antiSMASH-DB 4.0 matches for allorf_57691_57822

100.0% NC_014551.1and 13 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20935_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22080_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20760_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21830_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04855_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03875_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19015_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20390_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21915_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19785_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18870_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19495_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19845_lanthipeptide

Lanthipeptides: BAMF_RS41660_lanthipeptide

1

TAGWTLLTTLIGTSTISNSCNK

22

TAGWTLLTTLIGTSTISNSCNK

1

TAGWTLLTTLIGTSTISNSCNK

22

63.6% NZ_LN868937.1: Lanthipeptides: BN2144_RS19915_lanthipeptide

1

TAGW--TLLTTLIGTSTISNSC

20

TAGW T +T L+ TSTISN C

1

TAGWWFTSVTLLVTTSTISNHC

22

60.9% NZ_LN868937.1: Lanthipeptides: BN2144_RS20095_lanthipeptide

4

WTLLTTLIGTSTISNSCNK

22

+T+ TTLIGTSTISN+C K

5

YTIFTTLIGTSTISNNCGK

23

with 10 more sequence matches

59.1% NZ_LN868937.1: Lanthipeptides: BN2144_RS20100_lanthipeptide

4

WTLLTTLIGTSTISNSC

20

+T+ TTLIGTSTISN+C

5

YTIFTTLIGTSTISNNC

21

54.5% NC_014551.1and 27 others:

NC_014551.1 Lanthipeptides: BAMF_RS41665_lanthipeptideNC_014551.1 Lanthipeptides: BAMF_RS41670_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20950_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20940_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20945_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22090_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20775_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20765_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20770_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21840_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21835_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04865_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04860_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03890_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03880_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03885_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19010_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19005_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20385_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20380_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18880_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18875_lanthipeptideNZ_JALAUK010000033.1 Lanthipeptides: MOE38_RS18580_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00405_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19500_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19860_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19855_lanthipeptide

Lanthipeptides: BAMF_RS41675_lanthipeptide

4

WTLLTTLIGTSTISNSC

20

TL+TT++GTSTISN+C

2

LTLITTVVGTSTISNNC

18

54.5% NZ_CP026010.1and 18 others:

NZ_CP026010.1 Lanthipeptides: BS11774_RS22085_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21825_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21845_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04850_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04870_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19020_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19000_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21910_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21930_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21925_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21920_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19790_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18865_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18885_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00410_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19490_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19510_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19505_lanthipeptide

Lanthipeptides: BS11774_RS22075_lanthipeptide

4

WTLLTTLIGTSTISNSC

20

TL+TT++GTSTISN+C

2

LTLITTIVGTSTISNNC

18

50.0% NC_014551.1and 5 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20930_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20755_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03870_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20395_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19840_lanthipeptide

Lanthipeptides: BAMF_RS41655_lanthipeptide

4

WTLLTTLIGTSTISNSC

20

TL+TT++G+STISN+C

2

LTLITTIVGSSTISNNC

18

50.0% NZ_JAFFJY010000011.1: Lanthipeptides: JVX89_RS12320_lanthipeptide

3

GWTLLT-TLIGTSTISNSC

20

G+T T T +G STISN C

2

GYTPTTVTTVGLSTISNGC

20

40.9% NZ_CP066711.1: Lanthipeptides: JGZ56_RS07825_lanthipeptide

3

GWTLLTTLIGTSTISNSC

20

G + L + +G STISN C

5

GLSTLLSFLGGSTISNHC

22

40.9% NZ_FOHE01000012.1and 1 other:

NZ_FOHE01000012.1 Lanthipeptides: BMX40_RS20540_lanthipeptide

Lanthipeptides: BMX40_RS20545_lanthipeptide

3

GWTLLTTLIGTSTISNSC

20

GWT T+ ST+SN C

2

GWTWTVTIF-LSTVSNHC

18

34.6% NZ_CP003923.1and 4 others:

NZ_CP042163.1 Lanthipeptides: FPV17_RS21685_lanthipeptideNZ_CP117834.1 Lanthipeptides: PQ477_RS10640_lanthipeptideNZ_KV917380.1 Lanthipeptides: BK583_RS20905_lanthipeptideNZ_SNUX01000002.1 Lanthipeptides: E2L03_RS20340_lanthipeptide

Lanthipeptides: BLEG1_RS21170_lanthipeptide

9

TLIGTSTISNSC

20

T+IG ST SNSC

15

TVIGWSTASNSC

26

33.3% NZ_CP066711.1: Lanthipeptides: allorf_1652598_1652744_lanthipeptide

9

TLIGTSTISNSCNK

22

T+ G STISN CNK

14

TVYGLSTISNKCNK

27

17.2% NZ_FOHE01000012.1: Lanthipeptides: BMX40_RS20340_lanthipeptide

14

STISNSCNK

22

ST+SN+C++

19

STVSNNCDR

27

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg27_59 - Class III

Cleavage pHMM score: 0.00

RODEO score: 0

Monoisotopic mass: 1780.0 Da

Molecular weight: 1781.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1799.1 Da N = 1; 1817.1 Da N = 2; 1835.1 Da N = 3; 1853.1 Da N = 4; 1871.2 Da N = 5; 1889.2 Da N = 6

ctg27_60 - Class III

Cleavage pHMM score: 0.00

RODEO score: 0

Monoisotopic mass: 1723.0 Da

Molecular weight: 1724.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1742.0 Da N = 1; 1760.0 Da N = 2; 1778.1 Da N = 3; 1796.1 Da N = 4; 1814.1 Da N = 5; 1832.1 Da N = 6

allorf_56993_57124 - Class III

Cleavage pHMM score: 0.00

RODEO score: 0

Monoisotopic mass: 1780.0 Da

Molecular weight: 1781.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1799.1 Da N = 1; 1817.1 Da N = 2; 1835.1 Da N = 3; 1853.1 Da N = 4; 1871.2 Da N = 5; 1889.2 Da N = 6

allorf_57273_57401 - Class III

Cleavage pHMM score: 0.00

RODEO score: 0

Monoisotopic mass: 1723.0 Da

Molecular weight: 1724.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1742.0 Da N = 1; 1760.0 Da N = 2; 1778.1 Da N = 3; 1796.1 Da N = 4; 1814.1 Da N = 5; 1832.1 Da N = 6

allorf_57691_57822 - Class III

Cleavage pHMM score: -1.30

RODEO score: 0

Monoisotopic mass: 2119.2 Da

Molecular weight: 2120.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2138.4 Da N = 1; 2156.4 Da N = 2; 2174.4 Da N = 3; 2192.5 Da N = 4; 2210.5 Da N = 5; 2228.5 Da N = 6; 2246.5 Da N = 7; 2264.5 Da N = 8

gnlProkkaPIBGAFCF_35 - Region 1 - NRPS

Download region GenBank file

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Location: 1 - 9,790 nt. (total: 9,790 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 9790:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val - Asp)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg35_2

Search NORINE for peptide: strict or relaxed

AMP-binding (470..864): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code(s):: Val DAFWIGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1502..1904): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHIGK
Nearest Stachelhaus code(s):: Asp DLTKVGHIGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)