LOCUS       gnlProkkaPKJAKLKL_27    1323 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PKJAKLKL_27.
ACCESSION   gnlProkkaPKJAKLKL_27
VERSION     gnlProkkaPKJAKLKL_27
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2025-04-28 19:41:33
            Original ID  :: gnl|Prokka|PKJAKLKL_27
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 1323
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..1323
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[2:1322]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3..1322
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..1323
                     /SMILES="NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..1323
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /tool="antismash"
     aSDomain        complement(3..179)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg27_1_PCP.1"
                     /evalue="1.80E-25"
                     /label="ctg27_1_PCP.1"
                     /locus_tag="ctg27_1"
                     /protein_end="440"
                     /protein_start="381"
                     /score="80.8"
                     /tool="antismash"
                     /translation="ETLLSDIWQEVLGLEQIGVSDNFFTLGGDSIKGIQMASRLNQHGY
                     KLEMKDLFQHPTIE"
     aSModule        complement(3..1271)
                     /complete
                     /domains="nrpspksdomains_ctg27_1_AMP-binding.1"
                     /domains="nrpspksdomains_ctg27_1_PCP.1"
                     /locus_tags="ctg27_1"
                     /monomer_pairings="Leu -> Leu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS             complement(3..1322)
                     /NRPS_PKS="Domain: AMP-binding (17-276). E-value: 2.8e-69.
                     Score: 225.5. Matches aSDomain:
                     nrpspksdomains_ctg27_1_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (381-440). E-value: 1.8e-25. Score:
                     80.8. Matches aSDomain: nrpspksdomains_ctg27_1_PCP.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     245.4; E-value: 1.6e-74)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg27_1"
                     /sec_met_domain="AMP-binding (E-value: 1.2e-87, bitscore:
                     280.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3e-17, bitscore:
                     49.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDGSRSILSLPLDENDEANPQTETTADHLAYMIYTSGTTGQPKG
                     VMVEHHALVNLCFWHHDAFAMTADDKSAKYAGFGFDASIWEMFPTWTIGAELHVIDEAI
                     RLDITRLNHYFQEHGVTITFLPTQLAEQFMELENSSLRMLLVGGDKLKRAVKQPYTIVN
                     NYGPTENTVVATSGVINPEEDSLSIGRAIANTRAYILGDGDQVQPEGIAGELCVAGRGL
                     ARGYLNREEETAKRFTADPFVPGERMYRTGDLVKWNTQCGIEYIGRIDQQVKVRGYRIE
                     LSEIEVRLAQLAGVHDAAVTAVEDKAGNTALCAYVAPQQTDIEALKAALKDTLPDYMVP
                     AFWVEMDELPVTANGKIDKKALPSPDIEAGSAAYKAPETEMETLLSDIWQEVLGLEQIG
                     VSDNFFTLGGDSIKGIQMASRLNQHGYKLEMKDLFQHPTIE"
     CDS_motif       complement(87..116)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg27_1_0005"
                     /evalue="5.50E-03"
                     /label="PCP_mC"
                     /locus_tag="ctg27_1"
                     /protein_end="412"
                     /protein_start="402"
                     /score="10.2"
                     /tool="antismash"
                     /translation="NFFTLGGDSI"
     CDS_motif       complement(465..530)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg27_1_0004"
                     /evalue="7.90E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg27_1"
                     /protein_end="286"
                     /protein_start="264"
                     /score="37.1"
                     /tool="antismash"
                     /translation="YIGRIDQQVKVRGYRIELSEIE"
     aSDomain        complement(495..1271)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg27_1_AMP-binding.1"
                     /evalue="2.80E-69"
                     /label="ctg27_1_AMP-binding.1"
                     /locus_tag="ctg27_1"
                     /protein_end="276"
                     /protein_start="17"
                     /score="225.5"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="EANPQTETTADHLAYMIYTSGTTGQPKGVMVEHHALVNLCFWHHD
                     AFAMTADDKSAKYAGFGFDASIWEMFPTWTIGAELHVIDEAIRLDITRLNHYFQEHGVT
                     ITFLPTQLAEQFMELENSSLRMLLVGGDKLKRAVKQPYTIVNNYGPTENTVVATSGVIN
                     PEEDSLSIGRAIANTRAYILGDGDQVQPEGIAGELCVAGRGLARGYLNREEETAKRFTA
                     DPFVPGERMYRTGDLVKWNTQCGIEYIGRIDQQVKVR"
     CDS_motif       complement(612..701)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg27_1_0003"
                     /evalue="6.80E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg27_1"
                     /protein_end="237"
                     /protein_start="207"
                     /score="50.3"
                     /tool="antismash"
                     /translation="PEGIAGELCVAGRGLARGYLNREEETAKRF"
     CDS_motif       complement(804..833)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg27_1_0002"
                     /evalue="2.10E-03"
                     /label="NRPS-A_a5"
                     /locus_tag="ctg27_1"
                     /protein_end="173"
                     /protein_start="163"
                     /score="10.6"
                     /tool="antismash"
                     /translation="NYGPTENTVV"
     CDS_motif       complement(1173..1232)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg27_1_0001"
                     /evalue="2.70E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg27_1"
                     /protein_end="50"
                     /protein_start="30"
                     /score="35.1"
                     /tool="antismash"
                     /translation="AYMIYTSGTTGQPKGVMVEH"
ORIGIN
        1 tcttcgatgg tcgggtgctg gaagagatcc ttcatctcca gcttgtagcc gtgctggttc
       61 aggcggctcg ccatttggat gcctttgatc gagtcgccgc cgagcgtgaa gaaattatcg
      121 cttacgccga tctgttcaag gccgagcact tcctgccaaa tgtcagaaag cagcgtctcc
      181 atttccgttt ccggcgcttt gtacgcggcg cttcccgctt caatgtccgg cgacggcagg
      241 gcttttttgt caatctttcc gtttgcggtg accggaagct cgtccatctc cacccagaac
      301 gccggcacca tgtagtcagg aagggtatct ttcaacgccg ctttgagcgc ttcgatgtcc
      361 gtctgctgag gcgcgacata ggcgcaaagc gcggtattgc ccgctttgtc ttccaccgct
      421 gtcacggctg cgtcatgaac tcccgcaagc tgggcgaggc ggacttcaat ttctgaaagc
      481 tcgatccggt agccgcgcac tttgacctgc tggtcgatgc ggccgatgta ttcgatgccg
      541 cactgcgtgt tccatttgac gaggtcgccg gtccggtaca tgcgctcgcc gggcacaaac
      601 ggatcggcgg taaaccgctt cgccgtttct tcttcacggt tcaggtatcc gcgcgcaagc
      661 ccgcggcctg ccacgcacaa ttcaccggca atgccttccg gctgcacctg atcgccgtcg
      721 ccgagaatat aagctctcgt attggcaatc gcccgtccga ttgaaagcga atcttcctcc
      781 ggattgatca cgccgcttgt tgcgacgacg gtgttttccg tcgggccgta gttgttgacg
      841 atcgtgtacg gctgtttcac cgcccgcttc agcttgtcgc ctccgacgag aagcatgcgg
      901 agagagctat tttccagctc cataaactgt tcggccagct gcgtcggcag gaaggtgatc
      961 gtcacgccgt gctcttggaa atagtgattt aagcgggtga tatccagccg gatcgcttcg
     1021 tcaatgacgt gaagctccgc gccgatggtc catgtcggga acatctccca gatggaggcg
     1081 tcaaaaccga agcccgcgta tttggcgctc ttatcgtccg ccgtcatggc gaatgcgtcg
     1141 tggtgccaga agcacagatt cacgagcgca tggtgttcga ccatgacacc cttcggctgt
     1201 ccggtcgttc ccgacgtgta aatcatatac gcgaggtgat ccgccgttgt ttccgtctgc
     1261 gggttcgctt catcattttc atcaagcggc aggctcagaa tcgagcggct gccgtcaagc
     1321 aga
//
