GCA_036208815.1 - 14 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaPKJAKLKL_2 (original name was: gnl|Prokka|PKJAKLKL_2)

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	terpene	88,753	109,496
Region 2.2	PKS-like	194,203	228,030	butirosin A/butirosin B	Saccharide	7%

gnlProkkaPKJAKLKL_3 (original name was: gnl|Prokka|PKJAKLKL_3)

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	other	193,396	234,814	bacilysin	Other	100%

gnlProkkaPKJAKLKL_4 (original name was: gnl|Prokka|PKJAKLKL_4)

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	NRPS	10,292	37,952	surfactin	NRP:Lipopeptide	47%

gnlProkkaPKJAKLKL_5 (original name was: gnl|Prokka|PKJAKLKL_5)

Region	Type	From	To	Most similar known cluster		Similarity
Region 5.1	NRPS,transAT-PKS,betalactone	32,429	142,836	fengycin	NRP	93%

gnlProkkaPKJAKLKL_7 (original name was: gnl|Prokka|PKJAKLKL_7)

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	RiPP-like,NRP-metallophore,NRPS	14,569	66,344	bacillibactin	NRP	100%

gnlProkkaPKJAKLKL_8 (original name was: gnl|Prokka|PKJAKLKL_8)

Region	Type	From	To
Region 8.1	terpene	16,762	38,645
Region 8.2	T3PKS	93,868	134,968
Region 8.3	NRPS	481,626	527,153

gnlProkkaPKJAKLKL_9 (original name was: gnl|Prokka|PKJAKLKL_9)

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	lanthipeptide-class-iii	222,721	245,318

gnlProkkaPKJAKLKL_18 (original name was: gnl|Prokka|PKJAKLKL_18)

Region	Type	From	To	Most similar known cluster		Similarity
Region 18.1	transAT-PKS,T3PKS,NRPS	19,673	129,781	bacillaene	Polyketide+NRP	100%

gnlProkkaPKJAKLKL_19 (original name was: gnl|Prokka|PKJAKLKL_19)

Region	Type	From	To	Most similar known cluster		Similarity
Region 19.1	NRPS	1,835	27,187	surfactin	NRP:Lipopeptide	39%

gnlProkkaPKJAKLKL_27 (original name was: gnl|Prokka|PKJAKLKL_27)

Region	Type	From	To	Most similar known cluster		Similarity
Region 27.1	NRPS-like	1	1,323	thermoactinoamide A	NRP	100%

gnlProkkaPKJAKLKL_30 (original name was: gnl|Prokka|PKJAKLKL_30)

Region	Type	From	To	Most similar known cluster		Similarity
Region 30.1	NRPS	1	9,578	surfactin	NRP:Lipopeptide	8%

Region	Type	From	To	Most similar known cluster	Similarity
Region 2.1	terpene	88,753	109,496
Region 2.2	PKS-like	194,203	228,030	butirosin A/butirosin B	Saccharide	7%
Region 3.1	other	193,396	234,814	bacilysin	Other	100%
Region 4.1	NRPS	10,292	37,952	surfactin	NRP:Lipopeptide	47%
Region 5.1	NRPS,transAT-PKS,betalactone	32,429	142,836	fengycin	NRP	93%
Region 7.1	RiPP-like,NRP-metallophore,NRPS	14,569	66,344	bacillibactin	NRP	100%
Region 8.1	terpene	16,762	38,645
Region 8.2	T3PKS	93,868	134,968
Region 8.3	NRPS	481,626	527,153
Region 9.1	lanthipeptide-class-iii	222,721	245,318
Region 18.1	transAT-PKS,T3PKS,NRPS	19,673	129,781	bacillaene	Polyketide+NRP	100%
Region 19.1	NRPS	1,835	27,187	surfactin	NRP:Lipopeptide	39%
Region 27.1	NRPS-like	1	1,323	thermoactinoamide A	NRP	100%
Region 30.1	NRPS	1	9,578	surfactin	NRP:Lipopeptide	8%

Compact view

gnlProkkaPKJAKLKL_2 - Region 1 - terpene

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Location: 88,753 - 109,496 nt. (total: 20,744 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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No matches found.

gnlProkkaPKJAKLKL_2 - Region 2 - PKS-like

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Location: 194,203 - 228,030 nt. (total: 33,828 nt) Show pHMM detection rules used

Region on contig edge.

PKS-like: (t2pks2 or ksIII)

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gnlProkkaPKJAKLKL_3 - Region 1 - other

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Location: 193,396 - 234,814 nt. (total: 41,419 nt) Show pHMM detection rules used

other: (LmbU or Neocarzinostat or bcpB or frbD or mitE or vlmB or prnB or CaiA or bacilysin or orf2_PTase)

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gnlProkkaPKJAKLKL_4 - Region 1 - NRPS

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Location: 10,292 - 37,952 nt. (total: 27,661 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS modules

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		NT	AA		NT	AA

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 10291 - 37952:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Glu - Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg4_33

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AMP-binding (318..720): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: DAKDLGVVDK
Nearest Stachelhaus code(s):: Glu DAKDLGVVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1364..1761): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFMMGMVFK
Nearest Stachelhaus code(s):: Leu DAFMMGMVFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaPKJAKLKL_5 - Region 1 - NRPS,betalactone,transAT-PKS

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Location: 32,429 - 142,836 nt. (total: 110,408 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
betalactone: (HMGL-like and AMP-binding)

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 32428 - 142836:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Glu - D-Tyr) + (Ile) + (mal - Asn) + (D-Tyr - D-Asn - Gln - Pro) + (D-Asn - Ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 32428 - 109674:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - Asn) + (D-Tyr - D-Asn - Gln - Pro) + (D-Asn - Ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 91347 - 142836:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Glu - D-Tyr) + (Ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg5_64

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AMP-binding (268..663): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1778..2185): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg5_65

Search NORINE for peptide: strict or relaxed

AMP-binding (268..662): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DALSVGEVVK
Nearest Stachelhaus code(s):: Tyr DALSVGEVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1778..2173): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3287..3692): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: DAQDLGVVDK
Nearest Stachelhaus code(s):: Gln DAQDLGVVDK (100% 8Å match); Ile DAQDLGVVDK (74% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4334..4730): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code(s):: Pro DVQFIAHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg5_66

Search NORINE for peptide: strict or relaxed

CAL_domain (36..478): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 614.1
NH2: 18.8
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (2956..3351): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg5_67

PKS_AT (6..291): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 75.6
inactive: 33.5
Methoxymalonyl-CoA: 20.3
Propionyl-CoA: 13.0
Methylmalonyl-CoA: 11.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg5_84

AMP-binding (12..445): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: -GLHIAAPVK
Nearest Stachelhaus code(s):: Z-ahmohOrn FGLHIASTCK (56% 8Å match); Ile DGLFIGIPVK (32% 8Å match)
Stachelhaus code match:: 60% (weak)

ctg5_88

Search NORINE for peptide: strict or relaxed

AMP-binding (473..866): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFYGITFK
Nearest Stachelhaus code(s):: Ile DAFFYGITFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg5_89

Search NORINE for peptide: strict or relaxed

AMP-binding (10..239): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWHFGGVDK
Nearest Stachelhaus code(s):: Glu DAWHFGGVDK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (870..1262): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DGTITAEVAK
Nearest Stachelhaus code(s):: Tyr DGTITAEVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaPKJAKLKL_7 - Region 1 - NRP-metallophore,NRPS,RiPP-like

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Location: 14,569 - 66,344 nt. (total: 51,776 nt) Show pHMM detection rules used

Region on contig edge.

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))
NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 14568 - 66344:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 14616 - 66344:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - Thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg7_42

Search NORINE for peptide: strict or relaxed

AMP-binding (31..435): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVVNK
Nearest Stachelhaus code(s):: diOH-Bz PLPAQGVVNK (97% 8Å match); diOH-Bz or Sal PLPAQGVVNK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg7_44

Search NORINE for peptide: strict or relaxed

AMP-binding (456..855): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1513..1929): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaPKJAKLKL_8 - Region 1 - terpene

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Location: 16,762 - 38,645 nt. (total: 21,884 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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gnlProkkaPKJAKLKL_8 - Region 2 - T3PKS

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Location: 93,868 - 134,968 nt. (total: 41,101 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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NRPS/PKS substrates

NRPS/PKS substrate predictions

gnlProkkaPKJAKLKL_8 - Region 3 - NRPS

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Location: 481,626 - 527,153 nt. (total: 45,528 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

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Detailed domain annotation

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Similar known gene clusters from MIBiG 3.1

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 3, location 481625 - 527153:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (Cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg8_594

Search NORINE for peptide: strict or relaxed

AMP-binding (337..735): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DMFAVACNWK
Nearest Stachelhaus code(s):: Ph-Ser or Phe DAFTVAAIWK (47% 8Å match)
Stachelhaus code match:: 60% (weak)

ctg8_595

AMP-binding (215..605): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GMVLLAVVVK
Nearest Stachelhaus code(s):: Boz GMVLRAVVVK (65% 8Å match)
Stachelhaus code match:: 90% (moderate)

gnlProkkaPKJAKLKL_9 - Region 1 - lanthipeptide-class-iii

Download region GenBank file

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Location: 222,721 - 245,318 nt. (total: 22,598 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg9_228

ctg9_230

Leader:

MKDVLELQQLNEGTEVEASKGKGG

Core:

LLTLITTIVGSSTISNNC

Core with dehydrations:

LLDhbLIDhbDhbIVGDhaDhaDhbIDhaNNCys

MIBiG 3.1 matches for ctg9_230

34.8% BGC0002111 (multiple genes): Lanthipeptide: andalusicin A or andalusicin B

9

VGSSTISNNC

18

V +STISNNC

14

VWASTISNNC

23

30.4% BGC0002111 (EVG22_32245): Lanthipeptide: andalusicin A or andalusicin B

11

SSTISNNC

18

+STISNNC

16

ASTISNNC

23

antiSMASH-DB 4.0 matches for ctg9_230

100.0% NC_014551.1and 5 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20930_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20755_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03870_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20395_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19840_lanthipeptide

Lanthipeptides: BAMF_RS41655_lanthipeptide

1

LLTLITTIVGSSTISNNC

18

LLTLITTIVGSSTISNNC

1

LLTLITTIVGSSTISNNC

18

94.4% NZ_CP026010.1and 18 others:

NZ_CP026010.1 Lanthipeptides: BS11774_RS22085_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21825_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21845_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04850_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04870_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19020_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19000_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21910_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21930_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21925_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21920_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19790_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18865_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18885_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00410_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19490_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19510_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19505_lanthipeptide

Lanthipeptides: BS11774_RS22075_lanthipeptide

1

LLTLITTIVGSSTISNNC

18

LLTLITTIVG+STISNNC

1

LLTLITTIVGTSTISNNC

18

88.9% NC_014551.1and 27 others:

NC_014551.1 Lanthipeptides: BAMF_RS41675_lanthipeptideNC_014551.1 Lanthipeptides: BAMF_RS41665_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20945_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20950_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20940_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22090_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20770_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20775_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20765_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21840_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21835_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04865_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04860_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03885_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03890_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03880_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19010_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19005_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20380_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20385_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18880_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18875_lanthipeptideNZ_JALAUK010000033.1 Lanthipeptides: MOE38_RS18580_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00405_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19500_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19860_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19855_lanthipeptide

Lanthipeptides: BAMF_RS41670_lanthipeptide

1

LLTLITTIVGSSTISNNC

18

LLTLITT+VG+STISNNC

1

LLTLITTVVGTSTISNNC

18

with 12 more sequence matches

50.0% NC_014551.1and 13 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20935_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22080_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20760_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21830_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04855_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03875_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19015_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20390_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21915_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19785_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18870_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19495_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19845_lanthipeptide

Lanthipeptides: BAMF_RS41660_lanthipeptide

2

LTLITTIVGSSTISNNC

18

TL+TT++G+STISN+C

4

WTLLTTLIGTSTISNSC

20

50.0% NZ_LN868937.1and 1 other:

NZ_LN868937.1 Lanthipeptides: BN2144_RS20095_lanthipeptide

Lanthipeptides: BN2144_RS20100_lanthipeptide

1

LLTLITTIVGSSTISNNC

18

T+ TT++G+STISNNC

4

WYTIFTTLIGTSTISNNC

21

45.0% NZ_JAFFJY010000011.1: Lanthipeptides: JVX89_RS12320_lanthipeptide

7

TIVGSSTISNNC

18

T VG STISN C

9

TTVGLSTISNGC

20

40.9% NZ_LN868937.1: Lanthipeptides: BN2144_RS19915_lanthipeptide

1

LLTLITTIVGSSTISNNC

18

T +T +V +STISN+C

5

WFTSVTLLVTTSTISNHC

22

36.4% NZ_CP066711.1: Lanthipeptides: JGZ56_RS07825_lanthipeptide

2

LTLITTIVGSSTISNNC

18

L+ + + +G STISN+C

6

LSTLLSFLGGSTISNHC

22

34.8% NZ_CP035727.2and 5 others:

NZ_CP035727.2 Lanthipeptides: EVG22_RS16230_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16225_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16220_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16215_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16210_lanthipeptide

Lanthipeptides: EVG22_RS16235_lanthipeptide

9

VGSSTISNNC

18

V +STISNNC

14

VWASTISNNC

23

33.3% NZ_BMVC01000018.1and 6 others:

NZ_BMVC01000018.1 Lanthipeptides: IE282_RS34445_lanthipeptideNZ_BMVC01000018.1 Lanthipeptides: IE282_RS34455_lanthipeptideNZ_FOHE01000012.1 Lanthipeptides: BMX40_RS20340_lanthipeptideNZ_JALMDK010000006.1 Lanthipeptides: MZC64_RS26320_lanthipeptideNZ_JANBBG010000004.1 Lanthipeptides: allorf_1567890_1567982_lanthipeptideNZ_JANBBG010000004.1 Lanthipeptides: allorf_1567737_1567829_lanthipeptide

Lanthipeptides: IE282_RS34450_lanthipeptide

12

STISNNC

18

ST+SNNC

3

STVSNNC

9

30.8% NZ_CP003923.1and 4 others:

NZ_CP042163.1 Lanthipeptides: FPV17_RS21685_lanthipeptideNZ_CP117834.1 Lanthipeptides: PQ477_RS10640_lanthipeptideNZ_KV917380.1 Lanthipeptides: BK583_RS20905_lanthipeptideNZ_SNUX01000002.1 Lanthipeptides: E2L03_RS20340_lanthipeptide

Lanthipeptides: BLEG1_RS21170_lanthipeptide

2

LTLITTIVGSSTISNNC

18

+T T++G ST SN+C

10

ITTTRTVIGWSTASNSC

26

30.4% NZ_CP014806.1and 3 others:

NZ_CP014806.1 Lanthipeptides: ATY39_RS17975_lanthipeptideNZ_CP014806.1 Lanthipeptides: ATY39_RS17970_lanthipeptideNZ_CP014806.1 Lanthipeptides: ATY39_RS17965_lanthipeptide

Lanthipeptides: ATY39_RS17980_lanthipeptide

9

VGSSTISNNC

18

V +STISN+C

14

VFASTISNHC

23

30.4% NZ_CP035727.2: Lanthipeptides: EVG22_RS16240_lanthipeptide

11

SSTISNNC

18

+STISNNC

16

ASTISNNC

23

26.7% NZ_CM000752.1and 15 others:

NZ_CM000752.1 Sactipeptides: BTHUR0007_RS37430_sactipeptideNZ_JABGBR020000007.1 Sactipeptides: HOV72_RS15955_sactipeptideNZ_JABGBR020000007.1 Sactipeptides: HOV72_RS15950_sactipeptideNZ_JYPH01000039.1 Sactipeptides: TU52_RS33370_sactipeptideNZ_JYPH01000039.1 Sactipeptides: TU52_RS33365_sactipeptideNZ_NJPZ01000025.1 Sactipeptides: CE087_RS29070_sactipeptideNZ_NJPZ01000025.1 Sactipeptides: CE087_RS29065_sactipeptideNZ_NJPZ01000025.1 Sactipeptides: CE087_RS29060_sactipeptideNZ_NUBO01000020.1 Sactipeptides: CN575_RS31360_sactipeptideNZ_NUBO01000020.1 Sactipeptides: CN575_RS31355_sactipeptideNZ_NUBO01000020.1 Sactipeptides: CN575_RS31350_sactipeptideNZ_NUUQ01000016.1 Sactipeptides: COI65_RS30820_sactipeptideNZ_NUUQ01000016.1 Sactipeptides: COI65_RS30815_sactipeptideNZ_NUUQ01000016.1 Sactipeptides: COI65_RS30810_sactipeptideNZ_NUXK01000018.1 Sactipeptides: COJ27_RS31850_sactipeptide

Sactipeptides: BTHUR0007_RS37435_sactipeptide

1

LLTLITTIVGSSTIS

15

LL L+T G+ST S

16

LLNLVTAATGASTAS

30

26.7% NZ_CP066711.1: Lanthipeptides: allorf_1652598_1652744_lanthipeptide

7

TIVGSSTISNNC

18

T+ G STISN C

14

TVYGLSTISNKC

25

ctg9_231

Leader:

MRDVLELQQLSEGTEVEASKGKGG

ctg9_233

Leader:

MRDVLELQQLNEGTEVEASKGKGG

Shared core:

LLTLITTVVGTSTISNNCG

Core with dehydrations:

LLDhbLIDhbDhbVVGDhbDhaDhbIDhaNNCysG

MIBiG 3.1 matches for ctg9_231

34.8% BGC0002111 (multiple genes): Lanthipeptide: andalusicin A or andalusicin B

9

VGTSTISNNC

18

V STISNNC

14

VWASTISNNC

23

30.4% BGC0002111 (EVG22_32245): Lanthipeptide: andalusicin A or andalusicin B

11

TSTISNNC

18

STISNNC

16

ASTISNNC

23

antiSMASH-DB 4.0 matches for ctg9_231

100.0% NC_014551.1and 21 others:

NC_014551.1 Lanthipeptides: BAMF_RS41665_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20950_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20940_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22090_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20775_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20765_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21840_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21835_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04865_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04860_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03890_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03880_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19010_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19005_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20385_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18880_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18875_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00405_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19500_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19860_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19855_lanthipeptide

Lanthipeptides: BAMF_RS41675_lanthipeptide

1

LLTLITTVVGTSTISNNCG

19

LLTLITTVVGTSTISNNCG

1

LLTLITTVVGTSTISNNCG

19

94.7% NC_014551.1and 5 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20945_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20770_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03885_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20380_lanthipeptideNZ_JALAUK010000033.1 Lanthipeptides: MOE38_RS18580_lanthipeptide

Lanthipeptides: BAMF_RS41670_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

LLTLITTVVGTSTISNNC

1

LLTLITTVVGTSTISNNC

18

94.7% NZ_CP026010.1and 9 others:

NZ_CP054584.1 Lanthipeptides: HT135_RS21845_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04870_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19000_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21930_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21925_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21920_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18885_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19510_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19505_lanthipeptide

Lanthipeptides: BS11774_RS22085_lanthipeptide

1

LLTLITTVVGTSTISNNCG

19

LLTLITT+VGTSTISNNCG

1

LLTLITTIVGTSTISNNCG

19

with 22 more sequence matches

89.5% NZ_CP026010.1and 8 others:

NZ_CP054584.1 Lanthipeptides: HT135_RS21825_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04850_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19020_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21910_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19790_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18865_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00410_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19490_lanthipeptide

Lanthipeptides: BS11774_RS22075_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

LLTLITT+VGTSTISNNC

1

LLTLITTIVGTSTISNNC

18

84.2% NC_014551.1and 5 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20930_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20755_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03870_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20395_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19840_lanthipeptide

Lanthipeptides: BAMF_RS41655_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

LLTLITT+VG+STISNNC

1

LLTLITTIVGSSTISNNC

18

56.5% NZ_LN868937.1: Lanthipeptides: BN2144_RS20095_lanthipeptide

1

LLTLITTVVGTSTISNNCG

19

T+ TT++GTSTISNNCG

4

WYTIFTTLIGTSTISNNCG

22

54.5% NC_014551.1and 13 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20935_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22080_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20760_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21830_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04855_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03875_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19015_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20390_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21915_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19785_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18870_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19495_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19845_lanthipeptide

Lanthipeptides: BAMF_RS41660_lanthipeptide

2

LTLITTVVGTSTISNNC

18

TL+TT++GTSTISN+C

4

WTLLTTLIGTSTISNSC

20

54.5% NZ_LN868937.1: Lanthipeptides: BN2144_RS20100_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

T+ TT++GTSTISNNC

4

WYTIFTTLIGTSTISNNC

21

45.5% NZ_LN868937.1: Lanthipeptides: BN2144_RS19915_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

T +T +V TSTISN+C

5

WFTSVTLLVTTSTISNHC

22

45.0% NZ_JAFFJY010000011.1: Lanthipeptides: JVX89_RS12320_lanthipeptide

7

TVVGTSTISNNC

18

T VG STISN C

9

TTVGLSTISNGC

20

36.8% NC_016109.1and 5 others:

NC_016109.1 Lanthipeptides: KSE_RS43700_lanthipeptideNZ_CP068437.1 Lanthipeptides: JK628_RS03400_lanthipeptideNZ_CP068437.1 Lanthipeptides: JK628_RS03395_lanthipeptideNZ_CP068437.1 Lanthipeptides: allorf_0778008_0778106_lanthipeptideNZ_CP068437.1 Lanthipeptides: JK628_RS03405_lanthipeptide

Lanthipeptides: KSE_RS43695_lanthipeptide

12

STISNNCG

19

STISN+CG

2

STISNHCG

9

36.8% NC_016109.1: Lanthipeptides: KSE_RS43705_lanthipeptide

12

STISNNCG

19

STISN CG

2

STISNYCG

9

36.8% NZ_BMVC01000018.1and 1 other:

NZ_BMVC01000018.1 Lanthipeptides: IE282_RS34445_lanthipeptide

Lanthipeptides: IE282_RS34450_lanthipeptide

12

STISNNCG

19

ST+SNNCG

3

STVSNNCG

10

36.8% NZ_CP014222.1: Lanthipeptides: VN23_RS10390_lanthipeptide

7

TVVGTSTISNNCG

19

V ST+SN+CG

2

QVADWSTVSNHCG

14

36.4% NZ_CP066711.1: Lanthipeptides: JGZ56_RS07825_lanthipeptide

2

LTLITTVVGTSTISNNC

18

L+ + + +G STISN+C

6

LSTLLSFLGGSTISNHC

22

34.8% NZ_CP035727.2and 5 others:

NZ_CP035727.2 Lanthipeptides: EVG22_RS16230_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16225_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16220_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16215_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16210_lanthipeptide

Lanthipeptides: EVG22_RS16235_lanthipeptide

9

VGTSTISNNC

18

V STISNNC

14

VWASTISNNC

23

34.6% NZ_CP003923.1and 4 others:

NZ_CP042163.1 Lanthipeptides: FPV17_RS21685_lanthipeptideNZ_CP117834.1 Lanthipeptides: PQ477_RS10640_lanthipeptideNZ_KV917380.1 Lanthipeptides: BK583_RS20905_lanthipeptideNZ_SNUX01000002.1 Lanthipeptides: E2L03_RS20340_lanthipeptide

Lanthipeptides: BLEG1_RS21170_lanthipeptide

2

LTLITTVVGTSTISNNC

18

+T TV+G ST SN+C

10

ITTTRTVIGWSTASNSC

26

33.3% NZ_SMBT01000005.1: Lanthipeptides: EV682_RS11315_lanthipeptide

7

TVVGTSTISNNCG

19

T ST+SN+CG

1

TPADWSTVSNHCG

13

31.6% NZ_BMVC01000018.1and 4 others:

NZ_FOHE01000012.1 Lanthipeptides: BMX40_RS20340_lanthipeptideNZ_JALMDK010000006.1 Lanthipeptides: MZC64_RS26320_lanthipeptideNZ_JANBBG010000004.1 Lanthipeptides: allorf_1567890_1567982_lanthipeptideNZ_JANBBG010000004.1 Lanthipeptides: allorf_1567737_1567829_lanthipeptide

Lanthipeptides: IE282_RS34455_lanthipeptide

12

STISNNC

18

ST+SNNC

3

STVSNNC

9

31.6% NZ_CP113837.1and 1 other:

NZ_CP113837.1 Lanthipeptides: OU787_RS28895_lanthipeptide

Lanthipeptides: OU787_RS28905_lanthipeptide

12

STISNNCG

19

ST+SN+CG

2

STVSNHCG

9

31.6% NZ_CP113837.1: Lanthipeptides: OU787_RS28900_lanthipeptide

12

STISNNCG

19

ST+SN CG

2

STVSNYCG

9

30.4% NZ_CP014806.1and 3 others:

NZ_CP014806.1 Lanthipeptides: ATY39_RS17975_lanthipeptideNZ_CP014806.1 Lanthipeptides: ATY39_RS17970_lanthipeptideNZ_CP014806.1 Lanthipeptides: ATY39_RS17965_lanthipeptide

Lanthipeptides: ATY39_RS17980_lanthipeptide

9

VGTSTISNNC

18

V STISN+C

14

VFASTISNHC

23

30.4% NZ_CP035727.2: Lanthipeptides: EVG22_RS16240_lanthipeptide

11

TSTISNNC

18

STISNNC

16

ASTISNNC

23

30.0% NZ_CP066711.1: Lanthipeptides: allorf_1652598_1652744_lanthipeptide

7

TVVGTSTISNNC

18

TV G STISN C

14

TVYGLSTISNKC

25

13.2% NZ_JAIQCM010000003.1: Lanthipeptides: K9V56_RS08295_lanthipeptide

11

TSTISNNCG

19

TSTIS CG

33

TSTISGKCG

41

ctg9_232

Leader:

MRDVLELQQLNEGTEVEASKGKGG

Core:

LLTLITTVVGTSTISNNC

Core with dehydrations:

LLDhbLIDhbDhbVVGDhbDhaDhbIDhaNNCys

MIBiG 3.1 matches for ctg9_232

34.8% BGC0002111 (multiple genes): Lanthipeptide: andalusicin A or andalusicin B

9

VGTSTISNNC

18

V STISNNC

14

VWASTISNNC

23

30.4% BGC0002111 (EVG22_32245): Lanthipeptide: andalusicin A or andalusicin B

11

TSTISNNC

18

STISNNC

16

ASTISNNC

23

antiSMASH-DB 4.0 matches for ctg9_232

100.0% NC_014551.1and 27 others:

NC_014551.1 Lanthipeptides: BAMF_RS41675_lanthipeptideNC_014551.1 Lanthipeptides: BAMF_RS41665_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20945_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20950_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20940_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22090_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20770_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20775_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20765_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21840_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21835_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04865_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04860_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03885_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03890_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03880_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19010_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19005_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20380_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20385_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18880_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18875_lanthipeptideNZ_JALAUK010000033.1 Lanthipeptides: MOE38_RS18580_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00405_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19500_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19860_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19855_lanthipeptide

Lanthipeptides: BAMF_RS41670_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

LLTLITTVVGTSTISNNC

1

LLTLITTVVGTSTISNNC

18

89.5% NZ_CP026010.1and 18 others:

NZ_CP026010.1 Lanthipeptides: BS11774_RS22075_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21845_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21825_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04870_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04850_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19000_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19020_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21930_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21925_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21920_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21910_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19790_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18885_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18865_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00410_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19510_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19505_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19490_lanthipeptide

Lanthipeptides: BS11774_RS22085_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

LLTLITT+VGTSTISNNC

1

LLTLITTIVGTSTISNNC

18

88.9% NC_014551.1and 5 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20930_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20755_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03870_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20395_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19840_lanthipeptide

Lanthipeptides: BAMF_RS41655_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

LLTLITT+VG+STISNNC

1

LLTLITTIVGSSTISNNC

18

with 13 more sequence matches

54.5% NC_014551.1and 13 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20935_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22080_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20760_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21830_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04855_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03875_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19015_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20390_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21915_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19785_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18870_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19495_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19845_lanthipeptide

Lanthipeptides: BAMF_RS41660_lanthipeptide

2

LTLITTVVGTSTISNNC

18

TL+TT++GTSTISN+C

4

WTLLTTLIGTSTISNSC

20

52.2% NZ_LN868937.1and 1 other:

NZ_LN868937.1 Lanthipeptides: BN2144_RS20100_lanthipeptide

Lanthipeptides: BN2144_RS20095_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

T+ TT++GTSTISNNC

4

WYTIFTTLIGTSTISNNC

21

45.5% NZ_LN868937.1: Lanthipeptides: BN2144_RS19915_lanthipeptide

1

LLTLITTVVGTSTISNNC

18

T +T +V TSTISN+C

5

WFTSVTLLVTTSTISNHC

22

45.0% NZ_JAFFJY010000011.1: Lanthipeptides: JVX89_RS12320_lanthipeptide

7

TVVGTSTISNNC

18

T VG STISN C

9

TTVGLSTISNGC

20

36.4% NZ_CP066711.1: Lanthipeptides: JGZ56_RS07825_lanthipeptide

2

LTLITTVVGTSTISNNC

18

L+ + + +G STISN+C

6

LSTLLSFLGGSTISNHC

22

34.8% NZ_CP035727.2and 5 others:

NZ_CP035727.2 Lanthipeptides: EVG22_RS16230_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16225_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16220_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16215_lanthipeptideNZ_CP035727.2 Lanthipeptides: EVG22_RS16210_lanthipeptide

Lanthipeptides: EVG22_RS16235_lanthipeptide

9

VGTSTISNNC

18

V STISNNC

14

VWASTISNNC

23

34.6% NZ_CP003923.1and 4 others:

NZ_CP042163.1 Lanthipeptides: FPV17_RS21685_lanthipeptideNZ_CP117834.1 Lanthipeptides: PQ477_RS10640_lanthipeptideNZ_KV917380.1 Lanthipeptides: BK583_RS20905_lanthipeptideNZ_SNUX01000002.1 Lanthipeptides: E2L03_RS20340_lanthipeptide

Lanthipeptides: BLEG1_RS21170_lanthipeptide

2

LTLITTVVGTSTISNNC

18

+T TV+G ST SN+C

10

ITTTRTVIGWSTASNSC

26

33.3% NZ_BMVC01000018.1and 6 others:

NZ_BMVC01000018.1 Lanthipeptides: IE282_RS34445_lanthipeptideNZ_BMVC01000018.1 Lanthipeptides: IE282_RS34455_lanthipeptideNZ_FOHE01000012.1 Lanthipeptides: BMX40_RS20340_lanthipeptideNZ_JALMDK010000006.1 Lanthipeptides: MZC64_RS26320_lanthipeptideNZ_JANBBG010000004.1 Lanthipeptides: allorf_1567890_1567982_lanthipeptideNZ_JANBBG010000004.1 Lanthipeptides: allorf_1567737_1567829_lanthipeptide

Lanthipeptides: IE282_RS34450_lanthipeptide

12

STISNNC

18

ST+SNNC

3

STVSNNC

9

33.3% NZ_CP068437.1and 1 other:

NZ_CP068437.1 Lanthipeptides: JK628_RS03395_lanthipeptide

Lanthipeptides: JK628_RS03400_lanthipeptide

12

STISNNC

18

STISN+C

5

STISNHC

11

31.6% NZ_CP014222.1: Lanthipeptides: VN23_RS10390_lanthipeptide

7

TVVGTSTISNNC

18

V ST+SN+C

2

QVADWSTVSNHC

13

30.4% NZ_CP014806.1and 3 others:

NZ_CP014806.1 Lanthipeptides: ATY39_RS17975_lanthipeptideNZ_CP014806.1 Lanthipeptides: ATY39_RS17970_lanthipeptideNZ_CP014806.1 Lanthipeptides: ATY39_RS17965_lanthipeptide

Lanthipeptides: ATY39_RS17980_lanthipeptide

9

VGTSTISNNC

18

V STISN+C

14

VFASTISNHC

23

30.4% NZ_CP035727.2: Lanthipeptides: EVG22_RS16240_lanthipeptide

11

TSTISNNC

18

STISNNC

16

ASTISNNC

23

30.0% NZ_CP066711.1: Lanthipeptides: allorf_1652598_1652744_lanthipeptide

7

TVVGTSTISNNC

18

TV G STISN C

14

TVYGLSTISNKC

25

allorf_235724_235855

Leader:

MDEQMNEVLELQQLSKGSEVE

Core:

TAGWTLLTTLIGTSTISNSCNK

Core with dehydrations:

DhbAGWDhbLLDhbDhbLIGDhbDhaDhbIDhaNDhaCysNK

MIBiG 3.1 matches for allorf_235724_235855

31.8% BGC0000563 (AEA03263.1): Lanthipeptide: venezuelin

7

LTTLIGTSTISNSC

20

L TL+ T I SC

8

LLTLLNTVCIGISC

21

26.1% BGC0002111 (EVG22_32245): Lanthipeptide: andalusicin A or andalusicin B

13

TSTISNSC

20

STISN+C

16

ASTISNNC

23

26.1% BGC0002111 (multiple genes): Lanthipeptide: andalusicin A or andalusicin B

14

STISNSC

20

STISN+C

17

STISNNC

23

antiSMASH-DB 4.0 matches for allorf_235724_235855

100.0% NC_014551.1and 13 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20935_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22080_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20760_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21830_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04855_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03875_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19015_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20390_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21915_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19785_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18870_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19495_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19845_lanthipeptide

Lanthipeptides: BAMF_RS41660_lanthipeptide

1

TAGWTLLTTLIGTSTISNSCNK

22

TAGWTLLTTLIGTSTISNSCNK

1

TAGWTLLTTLIGTSTISNSCNK

22

63.6% NZ_LN868937.1: Lanthipeptides: BN2144_RS19915_lanthipeptide

1

TAGW--TLLTTLIGTSTISNSC

20

TAGW T +T L+ TSTISN C

1

TAGWWFTSVTLLVTTSTISNHC

22

60.9% NZ_LN868937.1: Lanthipeptides: BN2144_RS20095_lanthipeptide

4

WTLLTTLIGTSTISNSCNK

22

+T+ TTLIGTSTISN+C K

5

YTIFTTLIGTSTISNNCGK

23

with 10 more sequence matches

59.1% NZ_LN868937.1: Lanthipeptides: BN2144_RS20100_lanthipeptide

4

WTLLTTLIGTSTISNSC

20

+T+ TTLIGTSTISN+C

5

YTIFTTLIGTSTISNNC

21

54.5% NC_014551.1and 27 others:

NC_014551.1 Lanthipeptides: BAMF_RS41665_lanthipeptideNC_014551.1 Lanthipeptides: BAMF_RS41670_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20950_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20940_lanthipeptideNC_017188.1 Lanthipeptides: BAMTA208_RS20945_lanthipeptideNZ_CP026010.1 Lanthipeptides: BS11774_RS22090_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20775_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20765_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20770_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21840_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21835_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04865_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04860_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03890_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03880_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03885_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19010_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19005_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20385_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20380_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18880_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18875_lanthipeptideNZ_JALAUK010000033.1 Lanthipeptides: MOE38_RS18580_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00405_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19500_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19860_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19855_lanthipeptide

Lanthipeptides: BAMF_RS41675_lanthipeptide

4

WTLLTTLIGTSTISNSC

20

TL+TT++GTSTISN+C

2

LTLITTVVGTSTISNNC

18

54.5% NZ_CP026010.1and 18 others:

NZ_CP026010.1 Lanthipeptides: BS11774_RS22085_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21825_lanthipeptideNZ_CP054584.1 Lanthipeptides: HT135_RS21845_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04850_lanthipeptideNZ_CP061284.1 Lanthipeptides: ICJ61_RS04870_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19020_lanthipeptideNZ_CP065858.1 Lanthipeptides: I7X10_RS19000_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21910_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21930_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21925_lanthipeptideNZ_JAHVOC010000011.1 Lanthipeptides: KXD37_RS21920_lanthipeptideNZ_JALAPV010000012.1 Lanthipeptides: MOC91_RS19790_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18865_lanthipeptideNZ_JALAQA010000009.1 Lanthipeptides: MOD07_RS18885_lanthipeptideNZ_JALAVZ010000001.1 Lanthipeptides: MOF00_RS00410_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19490_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19510_lanthipeptideNZ_JANDGG010000010.1 Lanthipeptides: NKU38_RS19505_lanthipeptide

Lanthipeptides: BS11774_RS22075_lanthipeptide

4

WTLLTTLIGTSTISNSC

20

TL+TT++GTSTISN+C

2

LTLITTIVGTSTISNNC

18

50.0% NC_014551.1and 5 others:

NC_017188.1 Lanthipeptides: BAMTA208_RS20930_lanthipeptideNZ_CP032146.1 Lanthipeptides: D2M30_RS20755_lanthipeptideNZ_CP062984.1 Lanthipeptides: IL989_RS03870_lanthipeptideNZ_DF836084.1 Lanthipeptides: CMW1_RS20395_lanthipeptideNZ_KQ236689.1 Lanthipeptides: AD710_RS19840_lanthipeptide

Lanthipeptides: BAMF_RS41655_lanthipeptide

4

WTLLTTLIGTSTISNSC

20

TL+TT++G+STISN+C

2

LTLITTIVGSSTISNNC

18

50.0% NZ_JAFFJY010000011.1: Lanthipeptides: JVX89_RS12320_lanthipeptide

3

GWTLLT-TLIGTSTISNSC

20

G+T T T +G STISN C

2

GYTPTTVTTVGLSTISNGC

20

40.9% NZ_CP066711.1: Lanthipeptides: JGZ56_RS07825_lanthipeptide

3

GWTLLTTLIGTSTISNSC

20

G + L + +G STISN C

5

GLSTLLSFLGGSTISNHC

22

40.9% NZ_FOHE01000012.1and 1 other:

NZ_FOHE01000012.1 Lanthipeptides: BMX40_RS20540_lanthipeptide

Lanthipeptides: BMX40_RS20545_lanthipeptide

3

GWTLLTTLIGTSTISNSC

20

GWT T+ ST+SN C

2

GWTWTVTIF-LSTVSNHC

18

34.6% NZ_CP003923.1and 4 others:

NZ_CP042163.1 Lanthipeptides: FPV17_RS21685_lanthipeptideNZ_CP117834.1 Lanthipeptides: PQ477_RS10640_lanthipeptideNZ_KV917380.1 Lanthipeptides: BK583_RS20905_lanthipeptideNZ_SNUX01000002.1 Lanthipeptides: E2L03_RS20340_lanthipeptide

Lanthipeptides: BLEG1_RS21170_lanthipeptide

9

TLIGTSTISNSC

20

T+IG ST SNSC

15

TVIGWSTASNSC

26

33.3% NZ_CP066711.1: Lanthipeptides: allorf_1652598_1652744_lanthipeptide

9

TLIGTSTISNSCNK

22

T+ G STISN CNK

14

TVYGLSTISNKCNK

27

17.2% NZ_FOHE01000012.1: Lanthipeptides: BMX40_RS20340_lanthipeptide

14

STISNSCNK

22

ST+SN+C++

19

STVSNNCDR

27

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg9_230 - Class III

Cleavage pHMM score: 0.00

RODEO score: 0

Monoisotopic mass: 1723.0 Da

Molecular weight: 1724.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1742.0 Da N = 1; 1760.0 Da N = 2; 1778.1 Da N = 3; 1796.1 Da N = 4; 1814.1 Da N = 5; 1832.1 Da N = 6

ctg9_231 - Class III

Cleavage pHMM score: 0.00

RODEO score: 0

Monoisotopic mass: 1780.0 Da

Molecular weight: 1781.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1799.1 Da N = 1; 1817.1 Da N = 2; 1835.1 Da N = 3; 1853.1 Da N = 4; 1871.2 Da N = 5; 1889.2 Da N = 6

ctg9_232 - Class III

Cleavage pHMM score: 0.00

RODEO score: 0

Monoisotopic mass: 1723.0 Da

Molecular weight: 1724.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1742.0 Da N = 1; 1760.0 Da N = 2; 1778.1 Da N = 3; 1796.1 Da N = 4; 1814.1 Da N = 5; 1832.1 Da N = 6

ctg9_233 - Class III

Cleavage pHMM score: 0.00

RODEO score: 0

Monoisotopic mass: 1780.0 Da

Molecular weight: 1781.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1799.1 Da N = 1; 1817.1 Da N = 2; 1835.1 Da N = 3; 1853.1 Da N = 4; 1871.2 Da N = 5; 1889.2 Da N = 6

allorf_235724_235855 - Class III

Cleavage pHMM score: -1.30

RODEO score: 0

Monoisotopic mass: 2119.2 Da

Molecular weight: 2120.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2138.4 Da N = 1; 2156.4 Da N = 2; 2174.4 Da N = 3; 2192.5 Da N = 4; 2210.5 Da N = 5; 2228.5 Da N = 6; 2246.5 Da N = 7; 2264.5 Da N = 8

gnlProkkaPKJAKLKL_18 - Region 1 - NRPS,T3PKS,transAT-PKS

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Location: 19,673 - 129,781 nt. (total: 110,109 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
NRPS: cds(Condensation and (AMP-binding or A-OX))

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
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NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 19672 - 129781:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - ccmal - ohmal - mal) + (ccmal - mal - ohmal - mal) + (Me-ccmal - ohmal) + (Ala - ccmal - ccmal - Me-ccmal) + (mal - mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg18_34

PKS_AT (4..285): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 98.2
inactive: 43.3
Methoxymalonyl-CoA: 36.6
Methylmalonyl-CoA: 24.6
Propionyl-CoA: 24.5
2-Methylbutyryl-CoA: 5.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ctg18_35

PKS_AT (7..293): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 29.5
inactive: 17.6
Methoxymalonyl-CoA: 11.9
Benzoyl-CoA: 9.4
Isobutyryl-CoA: 8.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg18_36

PKS_AT (4..282): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 99.9
inactive: 30.9
Methoxymalonyl-CoA: 28.6
Methylmalonyl-CoA: 15.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg18_41

Search NORINE for peptide: strict or relaxed

CAL_domain (74..476): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 22.2
shikimic_acid: 0.0
NH2: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (1145..1540): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code(s):: Gly DILQLGMIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg18_44

Search NORINE for peptide: strict or relaxed

AMP-binding (494..898): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSNMAIIYK
Nearest Stachelhaus code(s):: Ala DVSNMAIIYK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaPKJAKLKL_19 - Region 1 - NRPS

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Location: 1,835 - 27,187 nt. (total: 25,353 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 1834 - 27187:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg19_26

Search NORINE for peptide: strict or relaxed

AMP-binding (469..867): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DGFFLGVVFK
Nearest Stachelhaus code(s):: Ile DGFFLGVVFK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaPKJAKLKL_27 - Region 1 - NRPS-like

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Location: 1 - 1,323 nt. (total: 1,323 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 1323:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg27_1

Search NORINE for peptide: strict or relaxed

AMP-binding (17..276): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaPKJAKLKL_30 - Region 1 - NRPS

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Location: 1 - 9,578 nt. (total: 9,578 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 9578:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val - Asp)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg30_2

Search NORINE for peptide: strict or relaxed

AMP-binding (470..864): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code(s):: Val DAFWIGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1502..1904): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHIGK
Nearest Stachelhaus code(s):: Asp DLTKVGHIGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)