LOCUS       c00027_gnlPro..         4567 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KFKHEOKF_27.
ACCESSION   c00027_gnlPro..
VERSION     c00027_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 21:12:19
            Original ID  :: gnl|Prokka|KFKHEOKF_27
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4567
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4567
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[585:3485]"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      586..3485
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..4567
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4567
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             complement(2..142)
                     /locus_tag="ctg27_1"
                     /transl_table=11
                     /translation="MALVLILISLIAILANNSHTVVGVKNYYKISSENSDYIHFSLDDF
                     GN"
     CDS             complement(96..299)
                     /locus_tag="ctg27_2"
                     /transl_table=11
                     /translation="MFSDNLLYLRNIKGLSQEQVAEAVGISRQSYSKWEQGDTFPNIDK
                     CDKLAKFYGVGFDIDIFDCYIS"
     CDS             586..1857
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg27_3"
                     /sec_met_domain="HGD-D (E-value: 2.8e-48, bitscore: 153.7,
                     seeds: 370, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLIELFGNHIYKTAPTNPLSARKKLCFAFSTAGLMCKYFPTKK
                     LLPSRQFMQWAAADSAITPLKTGKNSAIVSLYLPCEILHSMGISTLFPEALACYLAAAR
                     SEQIFIDTAEQNFVSQTMCSYHKAFIGMVETGVLPKPDFIINTTLACDANHLSFRRAAE
                     HYNVPQYMIDVPYNYSSGAVDYVAKQIRDMVTFIEKQGYTFDEAKLTETIEKSKQTLKN
                     FNDILALRANRSLSDEMTSQMLSVFATHVMLGTDNALKYSNDLKTELSAVPDGKKGVRL
                     LWVHTLPYWQDALRDLINFTDRCEIVACDMVMDAMYCDLEETDPYRFMADRLVRNTVNG
                     NGTNRINATLELAKKLNANGIVWYCHWGCKQTAGLSNIAKSTFESNGFPTLILDGDGCN
                     WNNVNDGQTVTRTEAFLELLENRK"
     CDS             1854..3485
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg27_4"
                     /sec_met_domain="HGD-D (E-value: 4.3e-18, bitscore: 54.5,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 6.8e-60, bitscore:
                     191.2, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIGYNCKYTPVEILACFDDYRLINNEAEHFDYSSQAMHSNVCSHI
                     KAICEEIHRGDYDKLVFMNCCDSTRRLFDVAESKIKYKTILDLPIDNGACAVKRLKNDL
                     LFLIKDLEAQTGKAFDAKEFLHNFSDKPIAFPNSKFILVLGANANGSLMNVIREAFQIP
                     VINMTCSNNRCVSNENMSENDSLDELMEKYARALMAQTPCMRMSDTTSRKQLLNNENLA
                     GIVYHTIKFCDYYCFEYTKLADSKTPILKIETDYTTQQLGQLSTRLEAFCETLNTENNS
                     KIKMNKNGKYYAGIDSGSTSTELVIFDENKNIVKAVMVRTGANAKAGAQKALDEAQISL
                     DEIKTITATGYGRKNIDFANDDVTEITCHARGARFLYPEARYIIDIGGQDSKFITLDEN
                     GNVASFVMNDKCAAGTGRFLENMAKALEISMEQMANDGLKYSEDISISNMCTVFAESEV
                     ISLISENKKIADIIHGLNKSVAIKTKALASKALGADGVMMTGGVANNKGVVTELEKQLN
                     TKIFISDKPEFCGAIGAGLFAVDSDR"
     CDS             complement(4014..4565)
                     /locus_tag="ctg27_5"
                     /transl_table=11
                     /translation="ALVLILISLIAILANNSHTVVGVKNYYKISSENSDYIHFSLDDFG
                     NYKNLYFQSNRIHSLMSDSYGSMFIAEYDKKSFNDQLLRLESFEYQTDVILDSDEYIIP
                     QTSFSIGDWNFKVLKPQSQTQEFPKNIDFVCINISECKIGYMTFYDEDIDYLCKSGNEN
                     EYMKHFVKKYFKYNFNSKVK"
ORIGIN
        1 aattcccaaa atcatctaaa gaaaaatgaa tataatccga attttcactt gatattttgt
       61 aatagttttt aacaccaaca actgtgtgtg aattattagc taatatagca atcaaagata
      121 tcaatatcaa aaccaacgcc ataaaacttt gccagcttat cgcatttatc aatgttcgga
      181 aaagtatctc cttgttccca tttggaatac gattgacgag aaattccaac tgcttcggca
      241 acttgttcct gcgataagcc tttaatgttt cttaaatata ataaattatc cgaaaacata
      301 aatacgcccc cttaactata aattattata gcattgaggg agcgtacttt ccaccatata
      361 aacttgtcag ataagtaatc cttttgttaa gtttaactgc atcggtcggc aatttatatt
      421 atctcaaacc attctgtgtg gcaagaaaat aattcattac gctgtttata cttcttctgt
      481 ttttatcgcc tttgcgcttg ccatcaatga agagaaaacg ctttgcgttt cgtatgttga
      541 tgtgctataa tcaaaattaa ataacacaag gaatactata acccaatgaa caatttgata
      601 gaattatttg gcaatcacat atataaaacc gcaccgacaa atcctttgtc agccagaaaa
      661 aagctgtgct ttgcatttag cacggcaggc cttatgtgca aatatttccc aacaaaaaag
      721 cttttgccgt cacgccaatt tatgcagtgg gcggctgccg attctgcaat tacaccgctg
      781 aaaacgggca aaaattctgc aatcgtaagc ctgtatctac cgtgtgaaat tttgcattcc
      841 atgggcatca gcacactgtt cccggaggct cttgcttgct atttggctgc cgcaaggtcg
      901 gagcagatat tcatcgacac ggcggagcaa aattttgttt cgcaaactat gtgctcttat
      961 cacaaggctt tcatcggaat ggtggaaaca ggcgttttgc ccaagccgga ttttatcatc
     1021 aacacaacac ttgcctgtga cgcaaatcac ttgtcgttca ggcgagcggc ggaacactac
     1081 aatgtgcctc aatatatgat tgatgtgccg tacaattatt caagcggtgc ggtggattat
     1141 gttgcaaagc aaatcagaga tatggtgacc tttattgaaa aacagggcta tacttttgat
     1201 gaggcaaagt tgactgaaac gattgaaaaa tcaaagcaga cgctaaagaa tttcaatgat
     1261 atattggcac ttcgtgcaaa tcgttcgctg tcggacgaaa tgacttctca aatgctttcg
     1321 gtttttgcaa cgcatgttat gctcggcacg gacaacgcat tgaaatacag taatgatttg
     1381 aaaaccgaac tttctgctgt tcccgatggc aaaaagggcg ttaggcttct ttgggtgcac
     1441 accttgcctt attggcagga cgctttgcgt gatttgatta acttcaccga ccgttgcgaa
     1501 attgtcgctt gcgatatggt tatggatgct atgtattgtg accttgaaga aaccgacccg
     1561 tacagattta tggcagaccg acttgtcaga aataccgtca acggaaacgg tacgaacaga
     1621 ataaacgcaa cgcttgaact tgcaaaaaag ctcaatgcaa acggcattgt ttggtactgc
     1681 cattggggtt gcaagcaaac ggcagggctt tcaaacattg caaaatccac ctttgagtca
     1741 aacggctttc caacgctcat tcttgacggt gacggctgta attggaacaa tgtcaacgac
     1801 ggtcaaaccg tgacgagaac agaagcgttt ttagaactgt tggagaatag aaaatgattg
     1861 gatataactg caaatacaca ccggtggaga ttttggcatg ctttgacgat tatcgtttaa
     1921 tcaacaacga agcggagcat tttgactatt cttcacaggc tatgcactca aatgtttgct
     1981 cacacatcaa ggcgatttgc gaggaaatac atcggggcga ctatgacaag cttgtattta
     2041 tgaactgctg tgacagcaca agacgattgt ttgatgtggc agaaagcaaa attaaataca
     2101 aaaccattct tgacctgccg attgataacg gcgcttgtgc cgtaaaacga ttaaagaatg
     2161 atttactttt tctcattaaa gaccttgaag cacagacggg caaagcgttt gacgcaaaag
     2221 aatttttgca caatttcagc gacaagccga ttgcgtttcc gaacagcaaa ttcattttag
     2281 tgcttggtgc aaatgcaaac ggtagcctga tgaatgtaat cagagaagcg tttcaaattc
     2341 ctgtaatcaa tatgacttgc tcaaataatc gctgtgtttc aaacgaaaat atgagcgaaa
     2401 atgacagtct tgacgagctg atggaaaaat acgcaagagc gttgatggcg caaacgcctt
     2461 gcatgcgaat gagcgacacg acatcaagaa aacaactgct aaacaacgaa aatttggcag
     2521 gtatcgtata ccacacaatc aagttttgcg attattattg ctttgaatat acaaagcttg
     2581 cagacagcaa gacaccgatt ttgaaaatcg aaacggacta cactacacag caattaggtc
     2641 agctttcaac aaggctggag gctttttgcg aaaccttgaa cacggagaat aattcaaaga
     2701 taaaaatgaa caaaaacgga aaatattacg caggaattga cagcggttca acctcaacgg
     2761 agcttgtcat ttttgacgaa aataagaata ttgtcaaggc tgttatggtc agaacaggtg
     2821 caaacgctaa ggcaggggcg caaaaggcac ttgacgaggc acaaatcagt cttgacgaaa
     2881 taaaaaccat aaccgcaaca ggctatggca gaaaaaatat tgattttgca aacgatgatg
     2941 taacggaaat cacttgccac gcaagagggg caaggttttt gtatcctgaa gcacgatata
     3001 ttattgacat cggcggacag gattcaaagt ttatcaccct tgatgaaaac ggcaatgttg
     3061 catcatttgt tatgaacgac aaatgcgcag caggcacggg cagattcctt gaaaatatgg
     3121 caaaggctct cgaaatcagt atggagcaaa tggcgaatga cggtttaaaa tacagcgagg
     3181 atatttcaat ttcaaatatg tgcacggtgt ttgccgaaag cgaagttata tcgctgattt
     3241 ccgaaaataa aaagatagcc gatattattc acggtttgaa caaaagcgtt gcgataaaga
     3301 cgaaggctct tgcaagcaag gcactcggtg cagacggtgt aatgatgaca ggcggtgttg
     3361 caaacaacaa gggcgttgta accgaacttg aaaagcagtt gaacacaaag attttcatat
     3421 ccgacaagcc tgaattttgc ggtgcaatcg gtgcgggatt gtttgccgtt gacagcgaca
     3481 gataaaccta atttcgcaga gaagcaaatg aacaagaact ttcagaaaaa acggcttacg
     3541 actatgtaaa gcagacgaaa aaaagaatct tgtttttttg aatgagaagt acgcccggca
     3601 cggacaaacg gtgctgttcg gcgactcaat cacggaaata ttcaatagct ataagctgtt
     3661 ttatgccttt tcgcaaacaa cggggcaggc agtttacaac cgtggaatca gcggtgacac
     3721 aagtgacaga ttgttggaaa ggctcgaatg taacgccttg aacataagcc cgaaaaatct
     3781 tgtgatttta atcggaacaa acgacatcgg cataggactt ccgcccgagt acaccttgaa
     3841 aaatattcaa gaaattttgc aaagaacgca gaagctctgc ccgaataccg ccttaaaaag
     3901 cagtattcgg gcataaaaat aggctgaaag taaaacttcc agccttgtag cagattatat
     3961 caacatgtca aaggtgaact gaattatccg taaattattt tttcaaaaag aaatcacttg
     4021 actttactat taaaattgta tttaaaatat tttttcacaa aatgtttcat atactcattt
     4081 tcgttgccgg atttacacaa ataatcaata tcctcgtcat aaaaggtcat ataaccaatt
     4141 ttacattcgg aaatgttaat gcaaacaaaa tctatatttt taggaaattc ctgtgtttgt
     4201 gactgtggtt ttaacacttt aaaattccaa tcaccaatgc taaaacttgt ttgcggtata
     4261 atatattcat cagaatcaag aataacatca gtttgatatt cgaaagattc gagtcgtaaa
     4321 agttgatcat taaagctttt tttgtcatat tcagcaataa acatactacc gtatgaatca
     4381 gacattaaag aatgtatcct gttcgactga aaatagagat ttttataatt cccaaaatca
     4441 tctaaagaaa aatgaatata atccgaattt tcacttgata ttttgtaata gtttttaaca
     4501 ccaacaactg tgtgtgaatt attagctaat atagcaatca aagatatcaa tatcaaaacc
     4561 aacgcca
//
