LOCUS       c00188_gnlPro..         8025 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BLDDJDFG_188.
ACCESSION   c00188_gnlPro..
VERSION     c00188_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 20:41:37
            Original ID  :: gnl|Prokka|BLDDJDFG_188
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8025
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..282
                     /locus_tag="ctg188_1"
                     /transl_table=11
                     /translation="IRFMGLSPLGFWQVQLLTSLMLFVSNALPNVAGMGSIETAFLLVF
                     GSFLKRGEVMSVLMLYRIASYYVVFAASAVGFFIAQRHLAQMEPPKEG"
     protocluster    1..8025
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1324:4316]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1325..4316
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..8025
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..8025
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             287..745
                     /locus_tag="ctg188_2"
                     /transl_table=11
                     /translation="MKSFHIPRTALFFILFALAAFAVLYHPIDLKALVRASSQGTLRTE
                     EVAHFGSTNDMDYHLLSMDSEAFQQTAELLSTVSCRKKLNQRYSAYTHDTFPVQSVTVS
                     FYDDAENQKFLLTVYSDGVCILNSAFVSVKYPGGGAAQLYEQLAELGQ"
     CDS             1325..3577
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg188_3"
                     /sec_met_domain="Gly_radical (E-value: 6.3e-42, bitscore:
                     131.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.3e-267, bitscore:
                     877.6, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIQFEQWEGFEGRIWKEEVNVRDFIQKNYTPYNGDESFLAGPTEA
                     TDKLWGALQQLQKAERAKGGVLDMETEVVSSLTSYGPGYIDENLKDLEQIVGLQTDKPL
                     KRAFMPYGGIKMAEQACTTYGYQPSAELHKIFTDYTRTHNQAVFDAYTPEMKKARHTHI
                     ITGLPDTYGRGRIVGDYRRVALYGIDALIQFKQEDLANCGDGTMTDDVIRLREEIARQI
                     SALKGMKKMAEAYGYDISQPAKDAKEACQWLYFGYLAAIKTQNGAAMSVGRISTFLDIY
                     IQRDLDKGILTESQAQELIDHMVMKFRMVKFARIPSYNQLFSGDPVWATLEVGGIGMDG
                     RSMVTKNCYRFLHTLENMGPAPEPNLTVLYSSALPEAFKKYAAKVSVNTSSVQYENDDV
                     MKPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKSHEQCGPNYAP
                     ITSEYLTYDEVLPKYVQMLDWLAGLYVNVLNLIQYMHDKYYYEEAEMALIDTDVRRTFA
                     TGIAGFSHVIDSLSAIKYAKVKVVRDESGLATGFETEGDFPKYGNDDDRADEIGVWLLK
                     TFLEMIKKRHTYRNSEATTSILTITSNVVYGKYTGALPDGRAAFTPFAPGATPSYGAEQ
                     NGLLASLNSVAKLPYHWALDGISNTQTINPEALGHSEDERVENLVQVLDGYFDQGAHHL
                     NVNVFGKEKLLDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVLARTCHGVL"
     CDS             3564..4316
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 62.2;
                     E-value: 2.1e-19)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg188_4"
                     /sec_met_domain="Radical_SAM (E-value: 5.2e-30, bitscore:
                     93.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASCKTLGYVHSLESFGSVDGPGVRFVVFLQGCALRCKYCHNPET
                     WAEGGEAWTAEALFQRVYRYRNYWGKKGGITVSGGEPLRQLDFLTEFFTLARAKSVHTA
                     LDTAGQPFRPDDPAYLAAFDRLMANTNLVILDLKEIDPERHRQLTGKDNANILAMARHI
                     SDLGIPLWIRHVLVPGLTDDEEGLRKTADFIRSLKTVQRVEVLPYHTLGLFKWQKLGIP
                     YPLPDAVPPTAEQVKRAEELLEVSRYPG"
     CDS             4889..6286
                     /locus_tag="ctg188_5"
                     /transl_table=11
                     /translation="MQIFNTLTRQKEEFVPQVPGEYRIYVCGPTVYNYIHIGNARPLIV
                     FDTLRRYLEYRGNKVIYVSNITDIDDKLIKKGQEEGTSMKEVAQRFEAEYLKDAAGLNC
                     EKPTVQPRATEHIPQILDIVKDLIDSGHAYVAKNGDVYFRVKSDPGYGKLSHLNLDDLE
                     SGNRELRSRMEDDLKEDPADFAVWKAAKPGEPAWESPYGMGRPGWHIECSAMSRTHLGK
                     TIDLHCGGQDLIFPHHENEIAQSECANGCEFARYWMHNGFINVDNQKMSKSLHNFFTVR
                     DVANLYGYEPIRYFMLTAGYRMPLNYTVDLIESCKNSLERLYTCRENLDFALSKTDFGT
                     DETLKEKAAEARTKFCTAMDDDLNTPDALAAVFDLVKEINTLSAASSKAALETAAAAFD
                     EITGVLGLLYNRKKDEVPAEVTALVEKRAAAKKAKDWATADAIRAQLTELGWAVKDTAQ
                     GPQLSKL"
     CDS             complement(6443..7660)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035:Iron-containing alcohol dehydrogenase (Score:
                     333.4; E-value: 4.9e-102)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg188_6"
                     /sec_met_domain="Fe-ADH (E-value: 4.8e-103, bitscore:
                     333.4, seeds: 219, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARFTLPRDLYHGKGALEALKSFTGKKAMICVGGGSMKRFGFLDR
                     AVEYLKEAGMEVELFEGIEPDPSVETVMRGAEAMLKFEPDWIIAMGGGSPIDAAKAMWI
                     KYEYPEITFEEMCKVFGIPPLRRKAHFCAISSTSGTATEVTAFSIITDYQKGIKYPIAD
                     FEITPDVAIVDPDLAETMPKKLVAHTGMDAMTHAVEAYVSTAHCDYTDPLAIFAIRMIQ
                     GDLVDSYNGDMSKRDSMHNAQCLAGMAFSNALLGIVHSMAHKTGAAFADYGAHIIHGAA
                     NAMYLPKVIAFNAKDPEAKKRYGVIADEMKLGGANDDEKVGLLIDYLRGMNDALNIPHC
                     IQHYGPDSYPTEQGFVPENVFLERLPEIAKNAIADACTGSNPRQPSQEEMEKLLKCCYY
                     DTEVDF"
ORIGIN
        1 atccggttca tggggctttc cccgctgggc ttctggcagg tgcagctgct caccagcctg
       61 atgctcttcg tttccaacgc tctgcccaac gtagccggga tgggctcgat cgaaacggcc
      121 tttttgctgg tgttcggcag ctttctgaaa cggggcgagg tgatgagcgt gctgatgctc
      181 taccgcatcg ccagctatta tgtggtattt gccgccagcg ccgtgggctt tttcatcgcc
      241 cagcggcatc tggcccaaat ggaaccacca aaggaggggt gagcagatga aaagcttcca
      301 cattccccgc accgctttat tttttatcct gtttgcactg gcagcttttg cggtgctata
      361 ccatcccatc gacctgaaag cgctggtgcg cgccagctcg cagggcacgc tccgcaccga
      421 ggaagtggcg cattttggca gcacaaacga catggactat cacctcctct ccatggacag
      481 cgaagcgttc caacaaacgg cagaattgct ttccaccgtc tcctgccgca aaaagctgaa
      541 ccagcgctat tctgcctaca cccacgatac ctttccggtg cagtcggtca ccgtcagctt
      601 ttatgacgat gccgagaacc agaagtttct gctgacggtg tattctgacg gcgtgtgcat
      661 cctgaacagc gcctttgtca gcgtaaaata ccccggcggc ggcgcagcac agctgtatga
      721 acaactggcg gagctggggc agtaaaaaca aaaatcgggg cactcaggtg gtcaagctga
      781 gtgtcccgtt tttgttttct ttgcgtcggc tgtggtacca aacacagccg gtgccgcgcc
      841 ggaagcagca gagcaaaccg tgctgccgct tcccgtagaa aagagaaagg aatggaggaa
      901 tggagcggag cgcttgcaga cgctcctttt ttctggtgca acgcggttag aattagctaa
      961 ccgccgctgc gagaacagga taccacagtc agttagtctt gtcaaccctt aatttacaaa
     1021 ttttttcgca aagttgctgc gccgccgggg tttcggcgtt ttttctacat tctgcccaaa
     1081 gaatagcctt tccgctggaa gaaaacccac ttgttttcta ttgcttttta aaacggttct
     1141 tgctattata ataacgcaag attttgtggg caggttttta tttttgtcta caccttgtag
     1201 taagggctgc cgcagacggc agcacaagac cgtttcgccc ccagaaaacc gcccagacag
     1261 agcggttttt caaaggctca ttagttagaa aacgctaact gcgtaatgga gcaaggaggt
     1321 tccaatgatc cagtttgaac agtgggaagg ctttgaaggc cgcatctgga aggaagaggt
     1381 caatgtgcgt gacttcatcc agaaaaacta caccccctat aacggcgacg aaagctttct
     1441 ggcaggcccc accgaggcta ccgataagct ttggggtgct ttgcagcagc tgcagaaggc
     1501 cgagcgcgcc aagggcggcg tgctggatat ggagaccgag gtggtcagca gcctgacctc
     1561 ctacggcccc ggctatattg acgaaaacct gaaggatctg gagcagatcg ttggcttaca
     1621 gaccgacaag cccctcaagc gcgcctttat gccctacggc ggcatcaaga tggcagagca
     1681 ggcctgcacc acctacggct accagccctc tgccgagctg cacaagatct ttaccgacta
     1741 cacccgcacc cacaatcagg ctgtgttcga tgcctacacc ccggagatga agaaggcacg
     1801 ccacacccac atcatcaccg gtctgccgga cacctacggc cgcggccgca tcgtgggcga
     1861 ctaccgccgc gttgccctgt atggcatcga tgccctgatc cagttcaagc aggaggatct
     1921 tgccaactgc ggcgacggca ccatgaccga cgatgtcatc cgtctgcgcg aggagatcgc
     1981 ccgccagatc agcgccctga agggcatgaa gaagatggct gaggcctacg gctacgacat
     2041 ctcccagccc gcaaaggacg ccaaggaggc ctgccagtgg ctgtacttcg gctatctggc
     2101 cgccatcaag acccagaacg gcgccgccat gagcgtgggc cgcatctcca ccttccttga
     2161 catttatatc cagcgcgatc tggacaaggg catcctgacc gaaagtcagg cacaggagct
     2221 catcgaccac atggtcatga agttccgcat ggtcaagttt gcccgcatcc ccagctacaa
     2281 ccagctgttc tccggtgacc ccgtgtgggc gacgctggag gtgggcggca tcggcatgga
     2341 cggccgcagc atggtcacca agaactgcta ccgcttcctg cacacactgg aaaacatggg
     2401 tcccgcaccg gagcccaacc tgaccgtgct gtactcctct gccctgcctg aggcctttaa
     2461 gaagtacgcc gccaaggtgt ccgtcaacac cagcagcgtc cagtacgaga acgacgatgt
     2521 gatgaagccc gtctggggcg acgattactc catctgctgc tgcgtgtctg ccacccaaac
     2581 cggcaaggag atgcagttct tcggcgcacg cgccaacctt gccaagtgcc tgctgtacgc
     2641 catcaacggc ggcgtggatg agaagagcca cgagcagtgt ggccccaact atgcccccat
     2701 caccagcgag tacctgacct atgacgaggt gctgcccaag tatgtgcaga tgctggactg
     2761 gctggcaggg ctgtacgtca acgtgctgaa cctcatccag tatatgcacg acaagtacta
     2821 ctacgaggaa gccgagatgg cactcatcga caccgatgtg cgccgcacct ttgccaccgg
     2881 cattgcaggc ttctcccacg tcatcgactc cctgagcgcc atcaagtacg ccaaggtgaa
     2941 ggtggtgcgc gacgagagcg gccttgccac cggctttgag accgagggcg acttccccaa
     3001 gtacggcaac gatgacgacc gcgccgacga gatcggcgtg tggctgctca agaccttcct
     3061 tgagatgatc aagaagcgcc acacctaccg caactccgag gctaccacct ccatcctgac
     3121 catcacctcc aacgtggtgt acggcaagta caccggcgct ctgccggatg gccgcgccgc
     3181 cttcaccccg tttgcccccg gcgctacccc cagctacggt gcagagcaga acggtctgct
     3241 ggcctcgctg aactccgtgg caaagctgcc ctaccactgg gcgctggacg gcatctccaa
     3301 cacccagacc atcaaccccg aggcactggg tcacagcgag gacgagcgcg tggagaatct
     3361 ggtgcaggta ctggatggct acttcgatca gggcgcacac cacctgaacg tgaatgtctt
     3421 tggcaaggag aagctgctgg acgccatgga gcaccctgag aaggaagagt acgccaactt
     3481 caccatccgc gtttccggct acgctgtcaa gttcatcgac ctgacccgcg agcagcagct
     3541 ggacgtgctg gcacgcacct gccatggcgt cctgtaagac ccttgggtac gtccactcgc
     3601 tggaatcctt cggctcggtg gacggccccg gcgtgcgctt tgtggtgttt ttgcaggggt
     3661 gtgccctgcg gtgcaagtac tgccacaacc ccgaaacatg ggcagagggc ggcgaggcgt
     3721 ggacggcaga agcgctgttc cagcgggtat accgctaccg caactactgg ggcaaaaagg
     3781 gcggcatcac ggtcagcggc ggcgagccgc tgcggcagct ggactttctg accgagttct
     3841 tcacgctggc gcgtgccaag agcgtgcaca cggcgctgga caccgccggg cagcccttcc
     3901 gcccggacga cccggcctat ctggccgcgt tcgaccgcct gatggctaac accaatctgg
     3961 tgatccttga cctcaaggag atcgacccgg agcgccaccg gcagctcacc ggcaaggaca
     4021 acgccaacat ccttgccatg gcgcggcaca tctccgacct cggcatcccg ctgtggatcc
     4081 ggcacgtcct cgtgcccggg ctgaccgatg acgaggaagg gctgcgcaaa acagcagact
     4141 ttatccgcag cctgaaaacg gtgcagcggg tggaggtgct gccctaccac accttggggc
     4201 tgttcaagtg gcagaagctg ggcatcccct atcccctgcc ggacgcagta ccgcccacgg
     4261 cagagcaggt aaagcgggca gaagaactgc tggaagtgag ccgctatccc gggtaaagca
     4321 ccaccgtttt gattgcgctc cgcttcgctc tgcgcaattt cagcggaaag aatatctatg
     4381 attttggccc ccggagcgtc tgcggacgct ccgggggccc ttttcacggg taaaaatggg
     4441 atatcggaac gcccgcaggc gttccgatat cccgaaattt aaaataattc ttccgctgag
     4501 gatgcccaga gcaacgcggc gggcattctg aatcgttctt tgttccctcc ttgacttccc
     4561 tgcaccgcac cgctataata aaggggtaaa aggataaatg cgtttatccg ggcagagtag
     4621 cgccgcacac cgtctgcgca gagagaacag gccactggct ggaagcctgt ttgcagaccg
     4681 ctgcgcaaag tgcgcccgga cagcacgcgg cgggaacccc gtggggaagt atcgccgtgc
     4741 ggtacgccgc cgttaccggc tttgagcgac aaacagaagt ggaaccgcga ttttgatttt
     4801 aaaaccgcct tcggagttgt gccttgtgca ccgctccggg ggcgttttgt tttgcggctt
     4861 ctgtttggaa caaaaaggag aattgaccat gcagatcttc aacaccctga cccgacagaa
     4921 ggaagaattt gtgccgcagg tgcccggcga gtaccgcatc tacgtctgcg gccctaccgt
     4981 gtacaactat atccacatcg gcaacgcacg tccgctgatc gtgttcgaca ctctgcgccg
     5041 ctatctggaa taccggggca acaaggtgat ctacgtttcc aacatcaccg atatcgacga
     5101 taagctcatc aagaaggggc aggaagaggg cacctccatg aaggaggtgg cacagcgctt
     5161 tgaggccgag tacctgaagg atgccgccgg tctgaactgc gaaaagccca ccgtgcagcc
     5221 ccgcgctacc gagcatatcc cccagatttt ggatatcgtc aaagatctga tcgactctgg
     5281 ccacgcctat gttgccaaga acggcgatgt gtacttccgg gtgaagagcg accccggcta
     5341 cggcaagctg agccacctga atctggacga tctggagagc ggcaaccgcg agctgcgcag
     5401 ccgcatggag gacgatctga aggaggatcc cgccgacttt gccgtctgga aggctgcaaa
     5461 gcccggtgag cccgcatggg aaagccccta cggcatgggc cgcccgggct ggcacatcga
     5521 gtgcagcgcc atgagccgca cccatctggg caagaccatc gacctgcact gcggcggtca
     5581 ggatctgatc ttcccccacc acgaaaacga gatcgcccag agcgagtgcg ccaacggctg
     5641 tgagtttgcc cgctactgga tgcacaacgg tttcatcaac gtggacaatc agaagatgtc
     5701 caagagcctg cacaacttct ttaccgtgcg ggatgtggca aacctctacg gctacgagcc
     5761 catccgctac ttcatgctga ccgccggcta ccgcatgccc ctgaactaca ccgtagacct
     5821 catcgagagc tgcaagaaca gtctggagcg cctgtacacc tgccgcgaga atctggactt
     5881 tgccctgagc aagaccgact tcggcaccga cgagaccctg aaggaaaagg ccgccgaggc
     5941 acgcaccaag ttctgcaccg ccatggacga cgacctgaac accccggatg cgcttgctgc
     6001 cgtgtttgat ctggtcaagg agatcaacac cctgtccgcc gcttccagca aggctgcact
     6061 ggagaccgct gccgccgcct ttgacgagat caccggtgtg ctgggtctgc tgtacaaccg
     6121 caagaaggat gaggtgcccg ccgaggtgac cgcgctggtt gagaagcgcg ctgccgccaa
     6181 gaaggccaag gactgggcca ccgccgatgc catccgcgca cagctgaccg aactgggctg
     6241 ggctgtcaag gacaccgcac agggtcccca gctgagcaag ctgtaacgcc ttagtagtat
     6301 gaaaggggtt cggaaacgcc gtaggcgttt ccgaacccct tttttcacgg gagaatgcag
     6361 gcgaacgcgg caaaagcccc gcagggcggt atgctctgca gggcttttgg cgctagggga
     6421 ggtgtaataa agaaaggacg gatcagaaat ccacctcagt gtcgtagtag cagcacttga
     6481 gcagcttctc catctcctcc tgactgggct ggcgggggtt ggagccggtg caggcgtcgg
     6541 cgatggcgtt cttggcgatc tcgggcagac gctccaggaa cacattctcc ggcacaaagc
     6601 cctgctcagt ggggtaggag tccgggccgt agtgctggat gcagtggggg atgttcagcg
     6661 cgtcgttcat gccgcgcaga tagtcgataa gcaagccgac cttttcgtca tcgtttgcgc
     6721 cgcccagctt catctcgtct gcgatgacgc cgtagcgctt tttggcctcg gggtccttgg
     6781 cgttgaaggc gatgacctta ggcaggtaca tggcgttggc cgcgccgtgg atgatgtgtg
     6841 cgccgtagtc tgcaaaggca gcgccggtct tgtgtgccat ggagtgcacg atgcccaaca
     6901 gtgcgttgga gaaagccata ccggcaaggc actgggcgtt gtgcatggag tcgcgcttgg
     6961 acatatcgcc attgtagctg tccaccagat cgccctggat catgcggatg gcgaagatgg
     7021 ccagcgggtc ggtgtagtcg cagtgcgcgg tggaaacata agcctccaca gcgtgagtca
     7081 tggcgtccat accggtgtgg gcgaccagct tcttgggcat ggtctcagcc agatccgggt
     7141 ccacgatggc cacgtccggg gtgatctcaa agtctgcgat ggggtacttg atgcccttct
     7201 ggtagtcggt gatgatggag aaagcggtca cctcggtggc agtgccggag gtggaggaaa
     7261 tggcgcagaa gtgtgccttg cggcgcagcg gcgggatgcc gaacaccttg cacatctcct
     7321 caaaggtgat ctcggggtac tcgtacttga tccacatggc cttggctgca tcgatgggag
     7381 agccgccgcc catggcaatg atccagtcgg gctcaaactt cagcatcgcc tcggcaccgc
     7441 gcatgacggt ctccacggaa ggatcgggtt cgatgccctc gaacagctcc acttccatgc
     7501 cggcttcctt caggtactcc acggcacgat ccaggaagcc gaaacgcttc atggagccgc
     7561 cgccgacaca gatcattgcc ttttttccgg tgaaggactt cagcgcttcc aaagcgccct
     7621 tgccatggta cagatcacgg ggcagagtaa aacgagccat aatacaaaac ctccaaaaat
     7681 agtacggcac acgcagcgct gtaccgcagt tcattattat gttaattata taacaatttg
     7741 gaggcagctt caattgacgt accgggagaa aaagtcgttc tattttgggc ggtttccaca
     7801 agcagtagga tgcaacaagc cccctgccct gaaaaaagcg caaaaaaaca ccccgaagtc
     7861 ctaagacttc gaggtgcttg cctttggagc tgctatccag attcgaactg gagacctcat
     7921 ccttaccaag gatgcgctct accaactgag ctatagcagc aaatggcgac ccggatcggg
     7981 ctcgaaccga cgacccccag cgtgacaggc tggtgctcta accaa
//
